Miniature transcription factors for the decoding of molecular basis for specific methylated DNA biorecognition
Supervisors:
Shiao Chow, University of Strathclyde
Brian Smith, School of Molecular Biosciences, University of Glasgow
Nicholas C. O. Tomkinson, University of Strathclyde
Summary:
This is an exciting interdisciplinary PhD studentship to develop synthetic tools to decode methylated DNA biorecognition and interactions.
DNA methylation underpins the maintenance of cellular identity and processes. Traditionally believed to be ‘silenced’, it has been discovered that DNA methylation could create new binding sites for transcription factors (TF) and trigger transcriptional activation. This project will study how methyl-sensitive TFs target and interact with specific methylated DNA site to understand their biorecognition basis in gene regulation.
Combining synthetic chemistry and NMR spectroscopy, this project will develop peptidomimetics inspired by methyl-sensitive transcription factors and profile their interactions with methylated DNA using advanced NMR spectroscopy. You will join the multidisciplinary research team led by Dr Shiao Chow (biomimetic development), Dr Brian Smith (NMR structural biology) and Prof Nick Tomkinson (chemical biology). You will be trained to become an expert in chemistry and structural biology with skills in computational modelling, synthetic chemistry, assay development and advanced NMR spectroscopy.
Due to the modular nature of the project, you will have the opportunity to explore and develop your own personalised areas of interests. As a member of the NORTHWESTBIO-DTP, you will receive specialised expertise, upskilling and leadership development through engagement with strategic partners.