Regulation of Macromolecular Condensate Dynamics Through G3BP1‑Mediated Nucleation and Staufen1‑Mediated Dissolution
Supervisors:
Dr Laura Spagnolo, University of Glasgow
Dr Massimo Vassalli, University of Glasgow
Dr Nick Robinson, Lancaster University
CASE INDUSTRY PARTNER: ConnectomX
Project Summary
Cells respond to environmental challenges by forming stress granules, membrane-less RNA-protein assemblies that help regulate gene expression during stress. Despite their importance in cellular adaptation and disease, the mechanisms controlling stress granule formation, organisation and dissolution remain poorly understood. This project will investigate how osmotic and mechanical stresses influence stress granule dynamics, focusing on the key regulatory proteins G3BP1 and STAUFEN1 (STAU1). The student will combine volume electron microscopy (vEM), Brillouin microscopy and single-molecule force spectroscopy to link molecular interactions with condensate architecture and cellular mechanical properties.
The project brings together expertise from the University of Glasgow, Lancaster University and industrial partner ConnectomX, providing a unique multidisciplinary training environment. The student will gain experience in advanced cell biology, RNA biology, electron microscopy, mechanobiology, optical tweezers, quantitative image analysis and data interpretation. Training will include the use of the BBSRC ALERT-funded Lumicks C-Trap at Lancaster University and a minimum three-month placement at ConnectomX, where the student will develop skills in vEM workflows, AI-assisted image analysis and research within a commercial setting.
Graduates will be equipped for careers in academia, biotechnology, pharmaceutical research, scientific instrumentation, imaging facilities, data science, and technology development, with a strong foundation in both discovery research and innovation.