Dr Laura Spagnolo

  • Reader (Molecular Biosciences)

Biography

Laura obtained her MSc in Pharmacy at the University of Trieste, Italy. She performed her doctoral studies at the University of Padova, Italy, and EMBL Heidelberg, Germany, under the supervision of Angelo Fontana and Luis Serrano. After obtaining her PhD in Pharmaceutical Sciences, Laura moved back to Trieste for a short postdoc at ELETTRA, the Italian synchrotron. Fascinated by the study of protein machines working on nucleic acids, she undertook postdoctoral work at the Institute of Cancer Research, with Laurence Pearl, contributing fundamental information on the structural organisation and macromolecular plasticity of DNA-PK synapses within Non-Homologous End-Joining complexes.
Laura established her own research group in 2009 at the University of Edinburgh, supported by an appointment at the Centre for Science at Extreme Conditions (www.csec.ed.ac.uk). At Edinburgh, the Spagnolo group continued work on DNA damage and repair, as well as starting new projects on gene editing machines. In 2016, Laura moved to Glasgow, where she is making good use of the excellent local electron microscopy resources to consolidate her studies.

Research interests

The overarching theme of the research conducted in my laboratory is centered on mechanisms of genome editing and engineering. We study macromolecular complexes involved in CRISPR-Cas interference, RNA metabolism and DNA damage repair with an integrative structural biology approach.

Publications

List by: Type | Date

Jump to: 2023 | 2022 | 2021 | 2020 | 2017 | 2015
Number of items: 7.

2023

Cannone, G., Kompaniiets, D., Graham, S., White, M. F. and Spagnolo, L. (2023) Structure of the Saccharolobus solfataricus type III-D CRISPR effector. Current Research in Structural Biology, 5, 100098. (doi: 10.1016/j.crstbi.2023.100098) (PMID:36843655) (PMCID:PMC9945777)

2022

Hogrel, G., Guild, A., Graham, S., Rickman, H., Grüschow, S., Bertrand, Q., Spagnolo, L. and White, M. F. (2022) Cyclic nucleotide-induced helical structure activates a TIR immune effector. Nature, 608(7924), pp. 808-812. (doi: 10.1038/s41586-022-05070-9) (PMID:35948638)

2021

van Beek, L., McClay, É., Patel, S., Schimpl, M., Spagnolo, L. and Maia de Oliveira, T. (2021) PARP power: a structural perspective on PARP1, PARP2, and PARP3 in DNA damage repair and nucleosome remodelling. International Journal of Molecular Sciences, 22(10), 5112. (doi: 10.3390/ijms22105112) (PMID:34066057) (PMCID:PMC8150716)

2020

Rennie, M. L. , Lemonidis, K. , Arkinson, C., Chaugule, V. K. , Clarke, M. , Streetley, J. , Spagnolo, L. and Walden, H. (2020) Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA. EMBO Reports, 21(7), e50133. (doi: 10.15252/embr.202050133) (PMID:32510829) (PMCID:PMC7332966)

Visentin, S., Cannone, G., Doutch, J., Harris, G., Gleghorn, M. L., Clifton, L., Smith, B. O. and Spagnolo, L. (2020) A multi-pronged approach to understanding the form and function of hStaufen protein. RNA, 26(3), pp. 265-277. (doi: 10.1261/rna.072595.119)

2017

Cannone, G., Visentin, S., Palud, A., Henneke, G. and Spagnolo, L. (2017) Structure of an octameric form of the minichromosome maintenance protein from the archaeon Pyrococcus abyssi. Scientific Reports, 7, 42019. (doi: 10.1038/srep42019) (PMID:28176822) (PMCID:PMC5296750)

2015

Cannone, G., Xu, Y., Beattie, T. R., Bell, S. D. and Spagnolo, L. (2015) The architecture of an Okazaki fragment-processing holoenzyme from the archaeon Sulfolobus solfataricus. Biochemical Journal, 465(2), pp. 239-245. (doi: 10.1042/BJ20141120) (PMID:25299633)

This list was generated on Sun Jun 4 05:49:01 2023 BST.
Jump to: Articles
Number of items: 7.

Articles

Cannone, G., Kompaniiets, D., Graham, S., White, M. F. and Spagnolo, L. (2023) Structure of the Saccharolobus solfataricus type III-D CRISPR effector. Current Research in Structural Biology, 5, 100098. (doi: 10.1016/j.crstbi.2023.100098) (PMID:36843655) (PMCID:PMC9945777)

Hogrel, G., Guild, A., Graham, S., Rickman, H., Grüschow, S., Bertrand, Q., Spagnolo, L. and White, M. F. (2022) Cyclic nucleotide-induced helical structure activates a TIR immune effector. Nature, 608(7924), pp. 808-812. (doi: 10.1038/s41586-022-05070-9) (PMID:35948638)

van Beek, L., McClay, É., Patel, S., Schimpl, M., Spagnolo, L. and Maia de Oliveira, T. (2021) PARP power: a structural perspective on PARP1, PARP2, and PARP3 in DNA damage repair and nucleosome remodelling. International Journal of Molecular Sciences, 22(10), 5112. (doi: 10.3390/ijms22105112) (PMID:34066057) (PMCID:PMC8150716)

Rennie, M. L. , Lemonidis, K. , Arkinson, C., Chaugule, V. K. , Clarke, M. , Streetley, J. , Spagnolo, L. and Walden, H. (2020) Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA. EMBO Reports, 21(7), e50133. (doi: 10.15252/embr.202050133) (PMID:32510829) (PMCID:PMC7332966)

Visentin, S., Cannone, G., Doutch, J., Harris, G., Gleghorn, M. L., Clifton, L., Smith, B. O. and Spagnolo, L. (2020) A multi-pronged approach to understanding the form and function of hStaufen protein. RNA, 26(3), pp. 265-277. (doi: 10.1261/rna.072595.119)

Cannone, G., Visentin, S., Palud, A., Henneke, G. and Spagnolo, L. (2017) Structure of an octameric form of the minichromosome maintenance protein from the archaeon Pyrococcus abyssi. Scientific Reports, 7, 42019. (doi: 10.1038/srep42019) (PMID:28176822) (PMCID:PMC5296750)

Cannone, G., Xu, Y., Beattie, T. R., Bell, S. D. and Spagnolo, L. (2015) The architecture of an Okazaki fragment-processing holoenzyme from the archaeon Sulfolobus solfataricus. Biochemical Journal, 465(2), pp. 239-245. (doi: 10.1042/BJ20141120) (PMID:25299633)

This list was generated on Sun Jun 4 05:49:01 2023 BST.

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Modernising the Electron Microscopy capabilities at the University of Glasgow
    Medical Research Council
    2022 - 2023
     
  • The directionality mechanism of DNA site-specific recombination by serine integrases
    Biotechnology and Biological Sciences Research Council
    2022 - 2025
     
  • CryoEM - SULSA
    Scottish Universities Life Sciences Alliance
    2018 - 2022
     
  • SMIC-SULSA
    Scottish Funding Council
    2018 - 2021
     
  • The Scottish Macromolecular Imaging Centre (SMIC)
    Medical Research Council
    2017 - 2022
     
  • Elucidating the molecular architecture of the Archaeal CMR complex, a key player in the unicellular immune response
    Biotechnology and Biological Sciences Research Council
    2016 - 2017
     
  • Determining the macromolecular structure and cellular function of an alternative MCM complex. University of St Andrews Reference: YMR025
    Medical Research Council
    2016 - 2017
     

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