Dynamic Multiomic Profiling of Single Sensory Neurons to Uncover Mechanisms of Plasticity
Supervisors:
Binoy Paulose Nadappuram, Department of Pure and Applied Chemistry, University of Strathclyde
Gregory Weir, School of Psychology and Neuroscience, University of Glasgow
Karen Faulds, Department of Pure and Applied Chemistry, University of Strathclyde
Summary:
This PhD project will investigate how sensory neurons adapt to external challenges such as excitation, inflammation, and injury, with a focus on their long axons and terminals where much of this adaptation occurs. Using cutting-edge nanotweezer technology, the student will perform high-resolution, repeated sampling of living neurons to profile RNA, lipid, and protein changes in sensory axons using RNA-Seq and Mass Spectrometry based methods. Experiments will be carried out in human iPSC-derived nociceptors and in fluorescently labelled sensory neurons within tissue preparations. By combining these two biological models, the project will provide unprecedented insight into the molecular mechanisms of sensory neuron plasticity and reveal how maladaptive changes may contribute to chronic pain.
The successful candidate will receive comprehensive, interdisciplinary training spanning molecular neuroscience, stem cell biology, advanced bioanalytical techniques, and multiomic data integration. They will gain hands-on expertise in emerging nanoscale technologies, as well as experience in experimental design, data analysis, and systems-level interpretation. Alongside technical skills, the student will develop transferable competencies in project management, teamwork, scientific communication, and engagement with ethical considerations in human-cell research.