Analytical Ultracentrifugation
Analytical ultracentrifugation (AUC) is a non-destructive method for probing the near-native solution conformation, size and assembly of macromolecules.
It can be used to determine if a sample is heterogenous, the molecular weight if it is pure enough for X-ray crystallography, NMR, SAXS or SANS.
If the sample binds to a ligand, the stoichiometry and Kd of the binding.
We have 2 Beckman Coulter AUCs: an XL-A that has absorbance optics (for chromophores that absorb between 180 and 800 nm) and an XL-I that additionally has Rayleigh interference optics that extend the dynamic range of concentrations observable and permit acquisition of data for systems that lack a chromophore.
Samples can be any of the following:
Proteins, Nucleic acids, Carbohydrates, Polymers, Colloids and complexes of any of the above
Sample requirements: normally we run a series of concentrations, to probe for concentration dependence.
Typical multiplexing: 3 or 7 sample holders/run
Up to 28 samples (e.g. concentrations) per run
Sample concentration
Absorbance optics: Aλ≈ 0.1-1.0 in 12 mm pathlength cell
λ = 180-800 nm
Interference optics: typically 0.05-30 mg/ml
Contact Dr. Mads Gabrielsen