Postgraduate research 

Biochemistry & Biotechnology PhD/iPhD/MSc (Research)

Biochemistry

Our research aims to answer fundamental questions about how cells and organisms work at the molecular and biochemical level. We study the structures and properties of DNA, RNA and protein molecules, and how these molecules interact within cells to form complex functional networks. We are also working towards applications of our knowledge to address important real-world problems.

Research projects

Self-funded PhD opportunities

Mechanisms and applications of DNA site-specific recombinases

Outline & aim

Site-specific recombinases are enzymes that promote rearrangements of DNA molecules, by cutting and rejoining DNA strands at precise places within short target sequences (sites). For example, a specific piece of DNA can be cut out of a larger molecule, or its orientation can be reversed. Our research group aims to understand in detail how recombinases catalyse these reactions, and how they are controlled. To do this we use high-resolution structural data and advanced techniques for laboratory analysis. Site-specific recombinases have tremendous potential as tools for manipulating DNA in the fields of biotechnology, synthetic biology and gene therapy. We are investigating how to engineer “designer recombinases” that are suitable for these purposes, and how to use them for novel applications.

The aim of the research project will be to advance our understanding in one of the areas outlined above. For example, the project might be an investigation of the mechanism of DNA strand cutting and rejoining, using novel “single-molecule” methodologies, or to develop novel designer recombinases suitable for targeting specific genes in a living organism for deletion or modification.

Techniques

  • analysis of high-resolution structures
  • protein expression, purification and biochemistry
  • methods for manipulation of DNA in E. coli
  • cloning, sequencing, sequence analysis
  • synthetic biology
  • novel methods for gene assembly
  • advanced methods for analysis of protein-DNA complexes, including single-molecule methods

References

  • Olorunniji, F.J. Rosser, S.J. and Stark, W.M. (2016) Site-specific recombinases: molecular machines for the Genetic Revolution. Biochem. J. 473, 673-684.
  • Olorunniji, F.J. et al. (2017). Control of serine integrase recombination directionality by fusion with the directionality factor . Nucleic Acids Res. 45, 8635-8645.
  • Proudfoot, C., McPherson, A.L., Kolb, A.F. and Stark, W.M. (2011) Zinc Finger recombinases with adaptable DNA sequence specificity. PLoS ONE 6, e19537.

Contact

Marshall.Stark@glasgow.ac.uk

Protein folding and secretion in mammalian cells

Outline & aim

The ability of cells to correctly fold and assemble proteins is the final stage in protein synthesis. Protein folding requires a subset of proteins able to either catalyse folding reactions or act as molecular chaperones preventing non-productive protein aggregation. The inability of cells to carry out the folding process results in some of the most catastrophic mammalian diseases such as cystic fibrosis, Alzheimer's and CJD.

Techniques

We aim to understand how cells fold and assemble proteins we are studying this process in mammalian cells using a combination of cell biological and biochemical techniques.

References

  • Tavender, T.J., Springate, J.S., and Bulleid, N.J. (2010) Recycling of peroxiredoxin IV provides a novel pathway for disulphide formation in the endoplasmic reticulum. The EMBO J., 29, 4185-4197.
  • Braakman I. and Bulleid N.J. (2011) Protein folding and modification in the mammalian endoplasmic reticulum. Annual Reviews in Biochemistry, 80: 71–99.
  • Oka O.B., Pringle, M.A., Schopp, I.M., Braakman, I., Bulleid, N.J. (2013) ERdj5 Is the ER Reductase that Catalyzes the Removal of Non-Native Disulfides and Correct Folding of the LDL Receptor. Mol Cell., 50(6):793-804.

Contact

Neil.Bulleid@glasgow.ac.uk

Overview

Our biochemists and molecular biologists study the “molecules of life”, the essential molecular components of all living organisms. We aim to understand how these molecules perform their functions, using a variety of modern molecular and biochemical approaches including structural analysis at the atomic level by X-ray crystallography, NMR spectrometry, and other biophysical methods. The knowledge gained by this research gives us opportunity to invent and develop novel ways of altering biological processes to our advantage, with applications in molecular medicine, biotechnology, synthetic biology, as well as industry.

PhD programmes in biochemistry and biotechnology will carry out a cutting-edge research project in an area that aligns with the expertise of one or more of our principal investigators in the fields of biochemistry and biotechnology. The subject of the project may be fundamental “blue skies” science or may be targeted at an important application. Projects may also be related to basic science and integrate with our existing research themes, while other projects are more focused on translational aspects of our research.

Some of our current research areas are:

  • cell signalling mechanisms in mammals, plants and insects
  • mitochondrial biogenesis and mitochondrial proteins
  • mechanisms of DNA sequence rearrangements
  • DNA sequences in human disease
  • genetic circuits and switches for synthetic biology
  • plant molecular biology
  • photosynthesis, plant photobiology, circadian factors in plants
  • structural determination by NMR and X-ray crystallography
  • structural bioinformatics, molecular modelling
  • drug receptors, molecular pharmacology
  • nuclear genomic architecture
  • mechanisms of intracellular trafficking
  • protein folding, targeting and modification
  • protein-protein and protein-DNA interactions
  • cell-surface interactions

Our PhD programme provides excellent training in cutting edge technologies that will be applicable to career prospects in both academia and industry. Many of our graduates become postdoctoral research associates while others go on to take up positions within industry, either locally or overseas. We have strong academic connections with many international collaborators in universities and research institutes.

Funds are available through the College of Medical, Veterinary and Life Sciences to allow visits to international laboratories where part of your project can be carried out. This provides an excellent opportunity for networking and increasing your scientific knowledge and skill set.

Study options

PhD

  • Duration: 3/4 years full-time; 5 years part-time

Individual research projects are tailored around the expertise of principal investigators.

MSc (Research)

  • Duration: 1 year full-time; 2 years part-time

Integrated PhD programmes (5 years)

  • Year 1 - completion of taught masters level modules
  • Years 2 to 5 -  research degree
  • International and UK/EU students can follow this route
  • International students with MSc and PhD scholarships/funding do not have to apply for 2 Visas or exit and re-enter the country between programmes. Students can move from MSc directly to a 1+3+1 PhD format (1 year MSc (taught only component), 3 years lab, 1 year thesis pending).
  • Before entering a research PhD, the completion of a taught MSc will give you an introduction to academic topics and research methods, training in laboratory skills and the critical evaluation of research data.
  • Our research-led teaching culture will allow you to sharpen your research ideas and discuss these with potential PhD supervisors during year 1. Upon successful completion of the taught component and dependent on your GPA, alongside students on our masters programmes, you will progress to your research degree in year 2 to do 3 years research / lab work and complete an examinable piece of independent research during the last year of the programme.
  • Please be aware that after having studied the taught component, your grades must meet our requirements in order to gain entry on to a PhD research programme. If not, you will receive the Masters degree only.
  • Also see Want to study an integrated PhD?: a step-by-step guide

Entry requirements

Awarded or expected First-class or high Upper Second-class BSc degree.

English Language requirements for applicants whose first language is not English.

Fees and funding

Fees

2020/21

  • £4,407 UK/EU
  • £21,920 outside EU

Prices are based on the annual fee for full-time study. Fees for part-time study are half the full-time fee.

Additional fees for all students:

  • Re-submission by a research student £525
  • Submission for a higher degree by published work £1,315
  • Submission of thesis after deadline lapsed £340
  • Submission by staff in receipt of staff scholarship £765

Depending on the nature of the research project, some students will be expected to pay a bench fee (also known as research support costs) to cover additional costs. The exact amount will be provided in the offer letter.

Alumni discount

A 10% discount is available to University of Glasgow alumni. This includes graduates and those who have completed a Junior Year Abroad, Exchange programme or International Summer School at the University of Glasgow. The discount is applied at registration for students who are not in receipt of another discount or scholarship funded by the University. No additional application is required.

Funding for EU students

The UK government has confirmed that EU nationals will remain eligible to apply for Research Council PhD studentships at UK institutions for 2019/20 to help cover costs for the duration of their study. The Scottish Government has confirmed that fees for EU students commencing their studies in 2019/20 and 2020/21 will be at the same level as those for UK students.

2019/20 fees

  • £4,327 UK/EU
  • £21,020 outside EU

Prices are based on the annual fee for full-time study. Fees for part-time study are half the full-time fee.

Additional fees for all students:

  • Re-submission by a research student £500
  • Submission for a higher degree by published work £1,250
  • Submission of thesis after deadline lapsed £320
  • Submission by staff in receipt of staff scholarship £730

Depending on the nature of the research project, some students will be expected to pay a bench fee (also known as research support costs) to cover additional costs. The exact amount will be provided in the offer letter.

Funding

Support

The College of Medical, Veterinary and Life Sciences Graduate School provides a vibrant, supportive and stimulating environment for all our postgraduate students. We aim to provide excellent support for our postgraduates through dedicated postgraduate convenors, highly trained supervisors and pastoral support for each student.
 
Our overarching aim is to provide a research training environment that includes:

  • provision of excellent facilities and cutting edge techniques
  • training in essential research and generic skills
  • excellence in supervision and mentoring
  • interactive discussion groups and seminars
  • an atmosphere that fosters critical cultural policy and research analysis
  • synergy between research groups and areas
  • extensive multidisciplinary and collaborative research
  • extensive external collaborations both within and beyond the UK 
  • a robust generic skills programme including opportunities in social and commercial training

How to apply

Identify potential supervisors

All Postgraduate Research Students are allocated a supervisor who will act as the main source of academic support and research mentoring. You may want to identify a potential supervisor and contact them to discuss your research proposal before you apply. Please note, even if you have spoken to an academic staff member about your proposal you still need to submit an online application form.

You can find relevant academic staff members with our staff research interests search.


Gather your documents

Before applying please make sure you gather the following supporting documentation:

  1. Final or current degree transcripts including grades (and an official translation, if needed) – scanned copy in colour of the original document
  2. Degree certificates (and an official translation, if needed): scanned copy in colour of the original document
  3. Two references on headed paper (academic and/or professional).
  4. Research proposal, CV, samples of written work as per requirements for each subject area.

Submitting References

To complete your application we will need two references (one must be academic the other can be academic or professional).

There are two options for you to submit references as part of your application.  You can upload a document as part of your application or you can enter in your referee’s contact details and we will contact them to request a reference.

Option 1 – Uploading as part of the application form

Your references should be on official headed paper. These should also be signed by the referee. You can then upload these via theOnline Application form with the rest your documents to complete the application process.

Please be aware that documents must not exceed 5MB in size and therefore you may have to upload your documents separately. The online system allow you to upload supporting documents only in PDF format. For a free PDF writer go to www.pdfforge.org.

Option 2 - Entering contact details as part of the application form

If you enter your referees contact details including email on the application form we will email them requesting they submit a reference once you have submitted the application form.  When the referee responds and sends a reference you will be sent an email to confirm the university has received this.

After submitting your application form

Use our Applicant Self Service uploading documents function to submit a new reference. We can also accept confidential references direct to rio-researchadmissions@glasgow.ac.uk, from the referee’s university or business email account.  


Apply now

I've applied. What next?

If you have any other trouble accessing Applicant Self-Service, please see Application Troubleshooting/FAQs. 


Contact us

International Students