BDFACS Canto II
New to the Facility as of September 2021 is Clyde, the BDFACS Canto II flow cytometer.
This machine has three lasers: Blue (488mn), Red (640nm) and Violet (405nm). It can detect 10 parameters simultaneously (8 colours plus forward scatter and side scatter).
Digital pulse sampling allows the measurement of height, area and width from all parameters simultaneously.
Data is acquired through BD FACSDiVaTM digital software.
Typical operation at 20,000 events/ second which requires a sample concentration of 2 x 107 cells/mL at an instrument flow rate of 60 μL/min.
The beauty of this machine is that filter and mirror changes can easily be made to customise the optical array for non standard experiments. Additionally we are able to switch off selected lasers as required for specialist applications.
For access to this machine please contact the Flow Core Facility Manager.
