Dr Alison Michie

  • Reader (Paul O'Gorman Leukaemia Research Centre)

Research interests

The Molecular Lymphopoiesis group was established in 2005 and focuses on the molecular events that induce haemopoietic stem cells (HSCs) to commit and differentiate towards the B and T lymphocyte lineages. During the course of our studies defining the role of specific signalling molecules that control the early stages of both B and T cell development, we established that PKC plays an essential role in mediating differentiation and proliferation signals downstream of the pre-TCR complex in vivo. An extension of these studies to investigate the role of PKC during B cell development revealed that inhibition of PKC signalling acts as an oncogenic trigger for developing B lymphocytes, resulting in the spontaneous generation of a population of cells that phenotypically resemble human B cell chronic lymphocytic leukaemia (CLL). Therefore our future studies aim to define the mechanisms that control normal lymphoid and leukaemic cell development using the experimental systems we have established.

Research Interests

We aim to identify molecular events that initiate the development and maintenance of CLL, which in turn will assist in the identification of novel therapeutic targets.  Validating of the importance of these novel targets in the development of CLL, in addition to testing of novel targeted compounds, will be carried out in patient-derived CLL samples. We have established an extensive cell bank, representative of the prognostic CLL patient subgroups to facilitate these studies. Moreover, we have developed in vitro culture conditions that mimic the pro-survival and pro-proliferative microenvironments found within lymphoid organs of patients where CLL cells persist.  These systems will provide critical information regarding the likely efficacy of novel therapeutic agents to induce CLL cell death in vivo.

We work with several academic collaborators including Professor Margaret Harnett (Glasgow), Professor Ewan Cameron (Glasgow), Dr. Mike Leach (Glasgow), Dr. David Vetrie (Glasgow), Dr. Paul Shiels (Glasgow), Dr. Helen Wheadon (Glasgow), Professor Martin Dyer (Leicester), Professor Ted Hupp (Edinburgh) and Professor Laurent Meijer (Roscoff).  We currently have industrial collaborations with GlaxoSmithKline.

Individual Projects:

• Define the molecular events that are triggered upon inhibition of PKC signalling;
• Determine whether subversion of PKC can initiate CLL in human CD34+ HSCs or mature B cells;
• Develop controllable and reproducible CLL models that can be used for testing potential novel drug combinations in future translational studies;
• Identify the molecular events that contribute towards CLL chemo-resistance in the lymphoid organ microenvironment;
• Investigate the signalling mechanisms that regulate B cell lineage commitment;
• Elucidate the negative regulatory mechanisms that govern early T cell development prior to pre-TCR complex expression.

 


Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Elucidating the mechanisms that regulate FOXO activity in chronic lymphocytic leukaemia a novel target for therapeutic exploitation?
    Bloodwise
    2019 - 2021
     
  • Elucidating the role of individual mTor complexes in the development and progression of chronic lymphocytic leukaemia
    Bloodwise
    2016 - 2019
     
  • Indentification of a gene signature within chronic lymphocytic leukaemia that establishes chemoresistance
    Tenovus Scotland
    2015 - 2016
     
  • Elucidating the role of mTor kinase signalling in chronic lymphocytic leukaemia
    The Kay Kendall Leukaemia Fund
    2014 - 2018
     
  • Regulation of PKC-beta gene expression in in CLL cells.
    Bloodwise
    2013 - 2016
     
  • Do CML stem cells actively evade the host immune response?
    Tenovus Scotland
    2013 - 2014
     
  • MICA: Determining the therapeutic potential of targeting mTORC-1/2 in chronic lymphocytic leukaemia - a pre-clinical study
    Medical Research Council
    2013 - 2016
     
  • Proof of principle: sLDL can deliver drugs to proliferating CLL cells within the bone marrow niche in vivo to reduce tumour burden
    Cancer Research UK
    2012 - 2013
     
  • Do CML stem cells actively evade the host immune response?
    Leuka
    2012 - 2013
     
  • Investigation of synthetic low-density lipoprotein (sLDL) as a novel drug delivery system to overcome chemoresistance of proliferating CLL cells.
    British Society for Haematology
    2011 - 2012
     
  • Defining the cellular and molecular characteristics of a quiescent, chemoresistant population of chronic lymphocytic leukaemia cells
    Tenovus Scotland
    2011 - 2012
     
  • Establishing a platform to investigate the potential of novel compounds for therapeutic development in chronic lymphocytic leukaemia
    Cancer Research UK
    2010 - 2012
     
  • Targeting proliferating CLL cells with a novel drug delivery system
    Cancer Research UK
    2010 - 2012
     
  • Development of a flow cytometry service within the Paul O'Gorman Leukaemia Research Centre
    The Kay Kendall Leukaemia Fund
    2010 - 2013
     
  • Fluorescence Activated Cell Sorting (FACS)
    The Howat Foundation Limited
    2010 - 2020
     
  • Investigating the potential of novel cyclin dependent kinase inhibitors as a novel therapeutic agents in the treatment of chronic lymphocytic leukaemia
    Scottish Executive Health Department
    2009 - 2010
     
  • Unique ErkMAPkinase checkpoint signatures drive differential responses during the immature-mature B cell transition?
    Medical Research Council
    2009 - 2012
     
  • Developing a novel therapeutic approach in chronic lymphocytic leukaemia
    Medical Research Council
    2008 - 2010
     
  • Developing a novel therapeutic approach in chronic lymphocytic leukaemia
    Tenovus Scotland
    2008 - 2009
     
  • Defining the cellular origin of chronic lymphocytic leukaemia
    Leukaemia & Lymphoma Research
    2007 - 2008
     
  • Defining the cellular origin of chronic lymphocytic leukaemia
    Medical Research Council
    2007 - 2010
     
  • Generating a human model system to study B cell-chronic lymphocytic lekaemia
    The Royal Society
    2006 - 2007
     
  • Investigation of signalling mechanisms that govern the primary checkpoint of T cell development in vivo
    Medical Research Council
    2001 - 2005
     

Publications

List by: Type | Date

Jump to: 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2007 | 2006 | 2005 | 2004 | 2002 | 2001 | 2000 | 1999 | 1998 | 1997 | 1996 | 1995 | 1993
Number of items: 53.

2018

Malik, N., Sansom, O. J. and Michie, A. M. (2018) The role of mTOR-mediated signals during haemopoiesis and lineage commitment. Biochemical Society Transactions, 46(5), pp. 1313-1324. (doi:10.1042/BST20180141) (PMID:30154096) (PMCID:PMC6195642)

Baquero, P. et al. (2018) Targeting quiescent leukemic stem cells using second generation autophagy inhibitors. Leukemia, (doi:10.1038/s41375-018-0252-4) (PMID:30185934) (Early Online Publication)

Holroyd, A. K. and Michie, A. M. (2018) The role of mTOR-mediated signaling in the regulation of cellular migration. Immunology Letters, 196, pp. 74-79. (doi:10.1016/j.imlet.2018.01.015) (PMID:29408410)

Michie, A.M. , Paunovic, V. and Harnett, M.M. (2018) Cellular homeostasis: Cell growth and cancer. In: Baynes, J. and Dominiczak, M. (eds.) Medical Biochemistry, 5th Edition. Elsevier: New York, NY. ISBN 9780702072994

2017

Kuntz, E. M., Baquero, P., Michie, A. M. , Dunn, K., Tardito, S., Holyoake, T. L., Helgason, G. V. and Gottlieb, E. (2017) Targeting mitochondrial oxidative phosphorylation eradicates therapy-resistant chronic myeloid leukemia stem cells. Nature Medicine, 23(10), pp. 1234-1240. (doi:10.1038/nm.4399) (PMID:28920959)

Cousins, A. F., Olivares, O., Michie, A. M. , Gottlieb, E. and Halsey, C. (2017) Association of CNS Involvement in Childhood Acute Lymphoblastic Leukaemia with Cholesterol Biosynthesis Upregulation. Spring Meeting for Clinician Scientists in Training 2017, London, UK, 23 Feb 2017. S35. (doi:10.1016/S0140-6736(17)30431-2)

Tarafdar, A. et al. (2017) CML cells actively evade host immune surveillance through cytokine-mediated downregulation of MHC-II expression. Blood, 129(2), pp. 199-208. (doi:10.1182/blood-2016-09-742049) (PMID:27793879) (PMCID:PMC5305055)

2016

Sinclair, A. et al. (2016) CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells. Blood, 128(3), pp. 371-383. (doi:10.1182/blood-2015-08-661785) (PMID:27222476) (PMCID:PMC4991087)

Middleton, O., Wheadon, H. and Michie, A. (2016) Classical complement pathway. In: Ratcliffe, M.J.H. (ed.) Encyclopedia of Immunobiology, 1st Edition. Elsevier: New York, NY. ISBN 9780123742797

Michie, A.M. , Tarafdar, A., Gallipoli, P., Pellicano, F., Hopcroft, L. , Korfi, K., Cassels, J., Jorgensen, H.J., Vetrie, D. and Holyoake, T.L. (2016) CML Cells Actively Evade Host Immune Surveillance Through Cytokine-Mediated Downregulation of MHC-I I Expression. 36th World Congress of the International Society of Hematology, Glasgow, Scotland, 18-21 Apr 2016. (doi:10.1111/bjh.14019)

Selman, C. , Sinclair, A., Pedroni, S. M.A., Irvine, E. E., Michie, A. M. and Withers, D. J. (2016) Evidence that hematopoietic stem cell function is preserved during aging in long-lived S6K1 mutant mice. Oncotarget, 7, 21. (doi:10.18632/oncotarget.8729) (PMID:27083004)

2015

Nakagawa, R. et al. (2015) Generation of a poor prognostic chronic lymphocytic leukemia-like disease model: PKC subversion induces up-regulation of PKC II expression in B lymphocytes. Haematologica, 100(4), pp. 499-510. (doi:10.3324/haematol.2014.112276) (PMID:25616575) (PMCID:PMC4380723)

O'Connor, C., Campos, J., Osinski, J. M., Gronostajski, R. M., Michie, A. and Keeshan, K. (2015) Nfix expression critically modulates early B lymphopoiesis and myelopoiesis. PLoS ONE, 10(3), e0120102. (doi:10.1371/journal.pone.0120102) (PMID:25780920) (PMCID:PMC4363787)

Middleton, O., Cosimo, E., Dobbin, E., McCaig, A.M., Clarke, C., Brant, A.M., Leach, M.T., Michie, A.M. and Wheadon, H. (2015) Complement deficiencies limit CD20 monoclonal antibody treatment efficacy in CLL. Leukemia, 29(1), pp. 107-114. (doi:10.1038/leu.2014.146) (PMID:24787488)

2014

Tarafdar, A. and Michie, A. M. (2014) Protein kinase C in cellular transformation: a valid target for therapy? Biochemical Society Transactions, 42(6), pp. 1556-1562. (doi:10.1042/BST20140255) (PMID:25399570)

O'Connor, C., Campos, J., Murphy, B., Hannon, M., Gronostajski, R., Michie, A. and Keeshan, K. (2014) NFIX influences stem and progenitor lineage fate. Experimental Hematology, 42(8), S54. (doi:10.1016/j.exphem.2014.07.205)

Michie, A.M. , Paunovic, V. and Harnett, M.M. (2014) Cellular homeostasis: cell growth and cancer. In: Baynes, J. and Dominiczak, M. (eds.) Medical Biochemistry, 4th Edition. Elsevier: New York, NY. ISBN 9781455745807

2013

Zhou, Y., Kurukuti, S., Saffrey, P., Vukovic, M., Michie, A.M. , Strogantsev, R., West, A.G. and Vetrie, D. (2013) Chromatin looping defines expression of TAL1, its flanking genes, and regulation in T-ALL. Blood, 122(26), pp. 4199-4209. (doi:10.1182/blood-2013-02-483875)

Keeshan, K. , O'Connor, C., Campos, J., Murphy, B., Hannon, M. and Michie, A. (2013) NFIX expression critically modulates early B lymphopoiesis and myelopoiesis. Experimental Hematology, 41(8), S68. (doi:10.1016/j.exphem.2013.05.267)

Cosimo, E. et al. (2013) Inhibition of NF-κB-mediated signaling by the cyclin-dependent kinase inhibitor CR8 overcomes pro-survival stimuli to induce apoptosis in chronic lymphocytic leukemia cells. Clinical Cancer Research, 19(9), pp. 2393-2405. (doi:10.1158/1078-0432.CCR-12-2170)

Schachtner, H. et al. (2013) Megakaryocytes assemble podosomes that degrade matrix and protrude through basement membrane. Blood, 121(13), pp. 2542-2552. (doi:10.1182/blood-2012-07-443457)

2012

Paunovic, V., Carter, N. A., Thalhamer, T., Blair, D., Gordon, B., Lacey, E., Michie, A. M. and Harnett, M. M. (2012) Immune complex-mediated co-ligation of the BCR with FcγRIIB results in homeostatic apoptosis of B cells involving Fas signalling that is defective in the MRL/Lpr model of systemic lupus erythematosus. Journal of Autoimmunity, 39(4), pp. 332-346. (doi:10.1016/j.jaut.2012.04.006)

Nakagawa, R., Vukovic, M., Cosimo, E. and Michie, A. M. (2012) Modulation of PKC-α promotes lineage reprogramming of committed B lymphocytes. European Journal of Immunology, 42(4), pp. 1005-1015. (doi:10.1002/eji.201141442)

McCaig, A. M., Cosimo, E., Leach, M. T. and Michie, A. M. (2012) Dasatinib inhibits CXCR4 signaling in chronic lymphocytic leukaemia cells and impairs migration towards CXCL12. PLoS ONE, 7(11), e48929. (doi:10.1371/journal.pone.0048929) (PMID:23133664) (PMCID:PMC3487834)

2011

McCaig, A. M., Cosimo, E., Leach, M. T. and Michie, A. M. (2011) Dasatinib inhibits B cell receptor signalling in chronic lymphocytic leukaemia but novel combination approaches are required to overcome additional pro-survival microenvironmental signals. British Journal of Haematology, 153(2), pp. 199-211. (doi:10.1111/j.1365-2141.2010.08507.x)

2010

Zhou, P., Hatziieremia, S., Elliott, M. A., Scobie, L., Crossan, C., Michie, A. M. , Holyoake, T. L., Halbert, G. W. and Jorgensen, H. G. (2010) Uptake of synthetic low density lipoprotein by leukemic stem cells — a potential stem cell targeted drug delivery strategy. Journal of Controlled Release, 148(3), pp. 380-387. (doi:10.1016/j.jconrel.2010.09.016)

Michie, A. M. , McCaig, A. M., Nakagawa, R. and Vukovic, M. (2010) Death-associated protein kinase (DAPK) and signal transduction: regulation in cancer. FEBS Journal, 277(1), pp. 74-80. (doi:10.1111/j.1742-4658.2009.07414.x)

2009

Copland, M. , Michie, A. M. and Holyoake, T. L. (2009) Stem cells in Leukemia and other hematological malignancies. In: Majumder, S. (ed.) Stem Cells and Cancer. Springer New York: New York, NY, pp. 111-136. ISBN 9780387896106 (doi:10.1007/978-0-387-89611-3_5)

2007

Michie, A. M. , Chan, A. C., Ciofani, M., Carleton, M., Lefebvre, J. M., He, Y., Allman, D. M., Wiest, D. L., Zuniga-Pflucker, J. C. and Izon, D. J. (2007) Constitutive Notch signalling promotes CD4(-)CD8(-) thymocyte differentiation in the absence of the pre-TCR complex, by mimicking pre-TCR signals. International Immunology, 19(12), pp. 1421-1430. (doi:10.1093/intimm/dxm113)

Michie, A.M. , Nakagawa, R. and McCaig, A.M. (2007) Murine models for chronic lymphocytic leukaemia. Biochemical Society Transactions, 35(5), pp. 1009-1012. (doi:10.1042/BST0351009)

2006

Michie, A.M. and Nakagawa, R. (2006) Elucidating the role of protein kinase C in chronic lymphocytic leukaemia. Hematological Oncology, 24(3), pp. 134-138. (doi:10.1002/hon.789)

Chaussepied, M., Moreau, M., Langsley, G., Michie, A.M. , Harnett, M. and Harnett, W. (2006) Notch is constitutively active in Theileria-transformed B cells and can be further stimulated by the filarial nematode-secreted product, ES-62. Microbes and Infection, 8(4), pp. 1189-1191. (doi:10.1016/j.micinf.2005.11.012)

Nakagawa, R., Soh, J.W. and Michie, A.M. (2006) Subversion of protein kinase C alpha signaling in hematopoietic progenitor cells results in the generation of a B-cell chronic lymphocytic leukemia-like population in vivo. Cancer Research, 66(1), pp. 527-534. (doi:10.1158/0008-5472.CAN-05-0841)

Nakagawa, R., Mason, S.M. and Michie, A.M. (2006) Determining the role of specific signaling molecules during lymphocyte development in vivo: instant transgenesis. Nature Protocols, 1(3), pp. 1185-1193. (doi:10.1038/nprot.2006.178)

2005

Michie, A.M. and Nakagawa, R. (2005) The link between PKC alpha regulation and cellular transformation. Immunology Letters, 96(2), pp. 155-162. (doi:10.1016/j.imlet.2004.08.013)

2004

Grady, G.C., Mason, S.M., Stephen, J., Zuniga-Pflucker, J.C. and Michie, A.M. (2004) Cyclic adenosine 5 '-monophosphate response element binding protein plays a central proliferation and differentiation role in mediating downstream of the pre-TCR complex in developing thymocytes. Journal of Immunology, 173(3), pp. 1802-1810. (doi:10.4049/jimmunol.173.3.1802) (PMID:15265911)

Ciofani, M., Schmitt, T.M., Ciofani, A., Michie, A.M. , Cuburu, N., Aublin, A., Maryanski, J.L. and Zuniga-Pflucker, J.C. (2004) Obligatory role for cooperative signaling by pre-TCR and notch during thymocyte differentiation. Journal of Immunology, 172(9), pp. 5230-5239.

2002

Michie, A.M. and Zuniga-Pflucker, J.C. (2002) Regulation of thymocyte differentiation: pre-TCR signals and beta-selection. Seminars in Immunology, 14(5), pp. 311-323. (doi:10.1016/S1044-5323(02)00064-7)

2001

Mariathasan, S., Zakarian, A., Bouchard, D., Michie, A. M. , Zúñiga-Pflücker, J. C. and Ohashi, P. S. (2001) Duration and strength of extracellular signal-regulated kinase signals are altered during positive versus negative thymocyte selection. Journal of Immunology, 167(9), pp. 4966-4973.

Michie, A.M. , Soh, J.-W., Hawley, R.G., Weinstein, I.B. and Zuniga-Pflucker, J.C. (2001) Allelic exclusion and differentiation by protein kinase C-mediated signals in immature thymocytes. Proceedings of the National Academy of Sciences of the United States of America, 98(2), pp. 609-614. (doi:10.1073/pnas.98.2.609)

2000

Kruisbeek, A. M., Haks, M. C., Carleton, M., Wiest, D. L., Michie, A. M. and Zúñiga-Pflücker, J. C. (2000) Branching out to gain control: how the pre-TCR is linked to multiple functions. Immunology Today, 21(12), pp. 637-644. (doi:10.1016/S0167-5699(00)01744-8)

Michie, A. M. , Carlyle, J. R., Schmitt, T. M., Ljutic, B., Cho, S. K., Fong, Q. and Zúñiga-Pflücker, J. C. (2000) Clonal characterization of a bipotent T cell and Natural Killer cell progenitor in the mouse fetal thymus. Journal of Immunology, 164(4), pp. 1730-1733.

Michie, A.M. and Zúñiga-Pflücker, J.C. (2000) In vivo detection of intracellular signalling pathways in developing thymocytes. Developmental Immunology, 8(1), pp. 31-45.

1999

Michie, A. M. , Trop, S., Wiest, D. L. and Zuniga-Pflucker, J. C. (1999) Extracellular Signal-Regulated Kinase (Erk) Activation by the Pre-T Cell Receptor in Developing Thymocytes in Vivo. Journal of Experimental Medicine, 190(11), pp. 1647-1656. (doi:10.1084/jem.190.11.1647)

Michie, A. M. and Zúñiga-Pflücker, J. C. (1999) Transfection and transcription of genes in developing thymocytes. In: Kearse, K.P. (ed.) T Cell Protocols: Development and Activation. Series: Methods in Molecular Biology, 134. Humana Press, pp. 55-62. ISBN 9780896038103 (doi:10.1385/1-59259-682-7:55)

1998

Michie, A. M. , Rena, G., Harnett, M. M. and Houslay, M. D. (1998) Upregulation of cAMP-specific PDE-4 activity following ligation of the TCR complex on thymocytes is blocked by selective inhibitors of protein kinase C and tyrosyl kinases. Cell Biochemistry and Biophysics, 28(2-3), pp. 161-185. (doi:10.1007/BF02737810)

Michie, A. M. , Carlyle, J. R. and Zúñiga-Pflücker, J. C. (1998) Early intrathymic precursor cells acquire a CD4low phenotype. Journal of Immunology, 160(4), pp. 1735-1741.

Carlyle, J. R., Michie, A. M. , Cho, S. K. and Zúñiga-Pflücker, J. C. (1998) Natural killer cell development and function precede ab T cell differentiation in mouse fetal thymic ontogeny. Journal of Immunology, 160(2), pp. 744-753. (PMID:9580246)

1997

Carlyle, J.R., Michie, A.M. , Furlonger, C., Nakano, T., Lenardo, M.J., Paige, C.J. and Zuniga-Pflucker, J.C. (1997) Identification of a novel developmental stage marking lineage commitment of progenitor thymocytes. Journal of Experimental Medicine, 186(2), pp. 173-182. (doi:10.1084/jem.186.2.173)

Michie, A.M. , Harnett, M.M. and Houslay, M.D. (1997) Cyclic nucleotide signalling throughout T cell maturation. In: Harnett, M.M. and Rigley, K.P. (eds.) Lymphocyte Signalling: Mechanism, Subversion and Manipulation. John Wiley & Sons Ltd.: Chichester, p. 247. ISBN 9780471959038

1996

Michie, A. M. , Lobban, M., Müller, T., Harnett, M. M. and Houslay, M. D. (1996) Rapid regulation of PDE-2 and PDE-4 cyclic AMP phosphodiesterase activity following ligation of the T cell antigen receptor on thymocytes: analysis using the selective inhibitors erythro-9-(2-hydroxy-3-nonyl)-adenine (EHNA) and rolipram. Cellular Signalling, 8(2), pp. 97-110. (doi:10.1016/0898-6568(95)02032-2)

1995

Houslay, M.D. et al. (1995) Alternative splicing of the type-IVA cyclic AMP phosphodiesterase gene provides isoform variants with distinct N-terminal domains fused to a common, soluble catalytic unit: ‘designer’ changes in Vmax, stability and membrane association. Biochemical Society Transactions, 23, pp. 393-398.

1993

Batty, I.H., Michie, A. , Fennel, M. and Downes, C.P. (1993) The characteristics, capacity and receptor regulation of inositol uptake in 1321N1 astrocytoma cells. Biochemical Journal, 294(1), pp. 49-55.

This list was generated on Sat Dec 15 19:30:22 2018 GMT.
Number of items: 53.

Articles

Malik, N., Sansom, O. J. and Michie, A. M. (2018) The role of mTOR-mediated signals during haemopoiesis and lineage commitment. Biochemical Society Transactions, 46(5), pp. 1313-1324. (doi:10.1042/BST20180141) (PMID:30154096) (PMCID:PMC6195642)

Baquero, P. et al. (2018) Targeting quiescent leukemic stem cells using second generation autophagy inhibitors. Leukemia, (doi:10.1038/s41375-018-0252-4) (PMID:30185934) (Early Online Publication)

Holroyd, A. K. and Michie, A. M. (2018) The role of mTOR-mediated signaling in the regulation of cellular migration. Immunology Letters, 196, pp. 74-79. (doi:10.1016/j.imlet.2018.01.015) (PMID:29408410)

Kuntz, E. M., Baquero, P., Michie, A. M. , Dunn, K., Tardito, S., Holyoake, T. L., Helgason, G. V. and Gottlieb, E. (2017) Targeting mitochondrial oxidative phosphorylation eradicates therapy-resistant chronic myeloid leukemia stem cells. Nature Medicine, 23(10), pp. 1234-1240. (doi:10.1038/nm.4399) (PMID:28920959)

Tarafdar, A. et al. (2017) CML cells actively evade host immune surveillance through cytokine-mediated downregulation of MHC-II expression. Blood, 129(2), pp. 199-208. (doi:10.1182/blood-2016-09-742049) (PMID:27793879) (PMCID:PMC5305055)

Sinclair, A. et al. (2016) CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells. Blood, 128(3), pp. 371-383. (doi:10.1182/blood-2015-08-661785) (PMID:27222476) (PMCID:PMC4991087)

Selman, C. , Sinclair, A., Pedroni, S. M.A., Irvine, E. E., Michie, A. M. and Withers, D. J. (2016) Evidence that hematopoietic stem cell function is preserved during aging in long-lived S6K1 mutant mice. Oncotarget, 7, 21. (doi:10.18632/oncotarget.8729) (PMID:27083004)

Nakagawa, R. et al. (2015) Generation of a poor prognostic chronic lymphocytic leukemia-like disease model: PKC subversion induces up-regulation of PKC II expression in B lymphocytes. Haematologica, 100(4), pp. 499-510. (doi:10.3324/haematol.2014.112276) (PMID:25616575) (PMCID:PMC4380723)

O'Connor, C., Campos, J., Osinski, J. M., Gronostajski, R. M., Michie, A. and Keeshan, K. (2015) Nfix expression critically modulates early B lymphopoiesis and myelopoiesis. PLoS ONE, 10(3), e0120102. (doi:10.1371/journal.pone.0120102) (PMID:25780920) (PMCID:PMC4363787)

Middleton, O., Cosimo, E., Dobbin, E., McCaig, A.M., Clarke, C., Brant, A.M., Leach, M.T., Michie, A.M. and Wheadon, H. (2015) Complement deficiencies limit CD20 monoclonal antibody treatment efficacy in CLL. Leukemia, 29(1), pp. 107-114. (doi:10.1038/leu.2014.146) (PMID:24787488)

Tarafdar, A. and Michie, A. M. (2014) Protein kinase C in cellular transformation: a valid target for therapy? Biochemical Society Transactions, 42(6), pp. 1556-1562. (doi:10.1042/BST20140255) (PMID:25399570)

O'Connor, C., Campos, J., Murphy, B., Hannon, M., Gronostajski, R., Michie, A. and Keeshan, K. (2014) NFIX influences stem and progenitor lineage fate. Experimental Hematology, 42(8), S54. (doi:10.1016/j.exphem.2014.07.205)

Zhou, Y., Kurukuti, S., Saffrey, P., Vukovic, M., Michie, A.M. , Strogantsev, R., West, A.G. and Vetrie, D. (2013) Chromatin looping defines expression of TAL1, its flanking genes, and regulation in T-ALL. Blood, 122(26), pp. 4199-4209. (doi:10.1182/blood-2013-02-483875)

Keeshan, K. , O'Connor, C., Campos, J., Murphy, B., Hannon, M. and Michie, A. (2013) NFIX expression critically modulates early B lymphopoiesis and myelopoiesis. Experimental Hematology, 41(8), S68. (doi:10.1016/j.exphem.2013.05.267)

Cosimo, E. et al. (2013) Inhibition of NF-κB-mediated signaling by the cyclin-dependent kinase inhibitor CR8 overcomes pro-survival stimuli to induce apoptosis in chronic lymphocytic leukemia cells. Clinical Cancer Research, 19(9), pp. 2393-2405. (doi:10.1158/1078-0432.CCR-12-2170)

Schachtner, H. et al. (2013) Megakaryocytes assemble podosomes that degrade matrix and protrude through basement membrane. Blood, 121(13), pp. 2542-2552. (doi:10.1182/blood-2012-07-443457)

Paunovic, V., Carter, N. A., Thalhamer, T., Blair, D., Gordon, B., Lacey, E., Michie, A. M. and Harnett, M. M. (2012) Immune complex-mediated co-ligation of the BCR with FcγRIIB results in homeostatic apoptosis of B cells involving Fas signalling that is defective in the MRL/Lpr model of systemic lupus erythematosus. Journal of Autoimmunity, 39(4), pp. 332-346. (doi:10.1016/j.jaut.2012.04.006)

Nakagawa, R., Vukovic, M., Cosimo, E. and Michie, A. M. (2012) Modulation of PKC-α promotes lineage reprogramming of committed B lymphocytes. European Journal of Immunology, 42(4), pp. 1005-1015. (doi:10.1002/eji.201141442)

McCaig, A. M., Cosimo, E., Leach, M. T. and Michie, A. M. (2012) Dasatinib inhibits CXCR4 signaling in chronic lymphocytic leukaemia cells and impairs migration towards CXCL12. PLoS ONE, 7(11), e48929. (doi:10.1371/journal.pone.0048929) (PMID:23133664) (PMCID:PMC3487834)

McCaig, A. M., Cosimo, E., Leach, M. T. and Michie, A. M. (2011) Dasatinib inhibits B cell receptor signalling in chronic lymphocytic leukaemia but novel combination approaches are required to overcome additional pro-survival microenvironmental signals. British Journal of Haematology, 153(2), pp. 199-211. (doi:10.1111/j.1365-2141.2010.08507.x)

Zhou, P., Hatziieremia, S., Elliott, M. A., Scobie, L., Crossan, C., Michie, A. M. , Holyoake, T. L., Halbert, G. W. and Jorgensen, H. G. (2010) Uptake of synthetic low density lipoprotein by leukemic stem cells — a potential stem cell targeted drug delivery strategy. Journal of Controlled Release, 148(3), pp. 380-387. (doi:10.1016/j.jconrel.2010.09.016)

Michie, A. M. , McCaig, A. M., Nakagawa, R. and Vukovic, M. (2010) Death-associated protein kinase (DAPK) and signal transduction: regulation in cancer. FEBS Journal, 277(1), pp. 74-80. (doi:10.1111/j.1742-4658.2009.07414.x)

Michie, A. M. , Chan, A. C., Ciofani, M., Carleton, M., Lefebvre, J. M., He, Y., Allman, D. M., Wiest, D. L., Zuniga-Pflucker, J. C. and Izon, D. J. (2007) Constitutive Notch signalling promotes CD4(-)CD8(-) thymocyte differentiation in the absence of the pre-TCR complex, by mimicking pre-TCR signals. International Immunology, 19(12), pp. 1421-1430. (doi:10.1093/intimm/dxm113)

Michie, A.M. , Nakagawa, R. and McCaig, A.M. (2007) Murine models for chronic lymphocytic leukaemia. Biochemical Society Transactions, 35(5), pp. 1009-1012. (doi:10.1042/BST0351009)

Michie, A.M. and Nakagawa, R. (2006) Elucidating the role of protein kinase C in chronic lymphocytic leukaemia. Hematological Oncology, 24(3), pp. 134-138. (doi:10.1002/hon.789)

Chaussepied, M., Moreau, M., Langsley, G., Michie, A.M. , Harnett, M. and Harnett, W. (2006) Notch is constitutively active in Theileria-transformed B cells and can be further stimulated by the filarial nematode-secreted product, ES-62. Microbes and Infection, 8(4), pp. 1189-1191. (doi:10.1016/j.micinf.2005.11.012)

Nakagawa, R., Soh, J.W. and Michie, A.M. (2006) Subversion of protein kinase C alpha signaling in hematopoietic progenitor cells results in the generation of a B-cell chronic lymphocytic leukemia-like population in vivo. Cancer Research, 66(1), pp. 527-534. (doi:10.1158/0008-5472.CAN-05-0841)

Nakagawa, R., Mason, S.M. and Michie, A.M. (2006) Determining the role of specific signaling molecules during lymphocyte development in vivo: instant transgenesis. Nature Protocols, 1(3), pp. 1185-1193. (doi:10.1038/nprot.2006.178)

Michie, A.M. and Nakagawa, R. (2005) The link between PKC alpha regulation and cellular transformation. Immunology Letters, 96(2), pp. 155-162. (doi:10.1016/j.imlet.2004.08.013)

Grady, G.C., Mason, S.M., Stephen, J., Zuniga-Pflucker, J.C. and Michie, A.M. (2004) Cyclic adenosine 5 '-monophosphate response element binding protein plays a central proliferation and differentiation role in mediating downstream of the pre-TCR complex in developing thymocytes. Journal of Immunology, 173(3), pp. 1802-1810. (doi:10.4049/jimmunol.173.3.1802) (PMID:15265911)

Ciofani, M., Schmitt, T.M., Ciofani, A., Michie, A.M. , Cuburu, N., Aublin, A., Maryanski, J.L. and Zuniga-Pflucker, J.C. (2004) Obligatory role for cooperative signaling by pre-TCR and notch during thymocyte differentiation. Journal of Immunology, 172(9), pp. 5230-5239.

Michie, A.M. and Zuniga-Pflucker, J.C. (2002) Regulation of thymocyte differentiation: pre-TCR signals and beta-selection. Seminars in Immunology, 14(5), pp. 311-323. (doi:10.1016/S1044-5323(02)00064-7)

Mariathasan, S., Zakarian, A., Bouchard, D., Michie, A. M. , Zúñiga-Pflücker, J. C. and Ohashi, P. S. (2001) Duration and strength of extracellular signal-regulated kinase signals are altered during positive versus negative thymocyte selection. Journal of Immunology, 167(9), pp. 4966-4973.

Michie, A.M. , Soh, J.-W., Hawley, R.G., Weinstein, I.B. and Zuniga-Pflucker, J.C. (2001) Allelic exclusion and differentiation by protein kinase C-mediated signals in immature thymocytes. Proceedings of the National Academy of Sciences of the United States of America, 98(2), pp. 609-614. (doi:10.1073/pnas.98.2.609)

Kruisbeek, A. M., Haks, M. C., Carleton, M., Wiest, D. L., Michie, A. M. and Zúñiga-Pflücker, J. C. (2000) Branching out to gain control: how the pre-TCR is linked to multiple functions. Immunology Today, 21(12), pp. 637-644. (doi:10.1016/S0167-5699(00)01744-8)

Michie, A. M. , Carlyle, J. R., Schmitt, T. M., Ljutic, B., Cho, S. K., Fong, Q. and Zúñiga-Pflücker, J. C. (2000) Clonal characterization of a bipotent T cell and Natural Killer cell progenitor in the mouse fetal thymus. Journal of Immunology, 164(4), pp. 1730-1733.

Michie, A.M. and Zúñiga-Pflücker, J.C. (2000) In vivo detection of intracellular signalling pathways in developing thymocytes. Developmental Immunology, 8(1), pp. 31-45.

Michie, A. M. , Trop, S., Wiest, D. L. and Zuniga-Pflucker, J. C. (1999) Extracellular Signal-Regulated Kinase (Erk) Activation by the Pre-T Cell Receptor in Developing Thymocytes in Vivo. Journal of Experimental Medicine, 190(11), pp. 1647-1656. (doi:10.1084/jem.190.11.1647)

Michie, A. M. , Rena, G., Harnett, M. M. and Houslay, M. D. (1998) Upregulation of cAMP-specific PDE-4 activity following ligation of the TCR complex on thymocytes is blocked by selective inhibitors of protein kinase C and tyrosyl kinases. Cell Biochemistry and Biophysics, 28(2-3), pp. 161-185. (doi:10.1007/BF02737810)

Michie, A. M. , Carlyle, J. R. and Zúñiga-Pflücker, J. C. (1998) Early intrathymic precursor cells acquire a CD4low phenotype. Journal of Immunology, 160(4), pp. 1735-1741.

Carlyle, J. R., Michie, A. M. , Cho, S. K. and Zúñiga-Pflücker, J. C. (1998) Natural killer cell development and function precede ab T cell differentiation in mouse fetal thymic ontogeny. Journal of Immunology, 160(2), pp. 744-753. (PMID:9580246)

Carlyle, J.R., Michie, A.M. , Furlonger, C., Nakano, T., Lenardo, M.J., Paige, C.J. and Zuniga-Pflucker, J.C. (1997) Identification of a novel developmental stage marking lineage commitment of progenitor thymocytes. Journal of Experimental Medicine, 186(2), pp. 173-182. (doi:10.1084/jem.186.2.173)

Michie, A. M. , Lobban, M., Müller, T., Harnett, M. M. and Houslay, M. D. (1996) Rapid regulation of PDE-2 and PDE-4 cyclic AMP phosphodiesterase activity following ligation of the T cell antigen receptor on thymocytes: analysis using the selective inhibitors erythro-9-(2-hydroxy-3-nonyl)-adenine (EHNA) and rolipram. Cellular Signalling, 8(2), pp. 97-110. (doi:10.1016/0898-6568(95)02032-2)

Houslay, M.D. et al. (1995) Alternative splicing of the type-IVA cyclic AMP phosphodiesterase gene provides isoform variants with distinct N-terminal domains fused to a common, soluble catalytic unit: ‘designer’ changes in Vmax, stability and membrane association. Biochemical Society Transactions, 23, pp. 393-398.

Batty, I.H., Michie, A. , Fennel, M. and Downes, C.P. (1993) The characteristics, capacity and receptor regulation of inositol uptake in 1321N1 astrocytoma cells. Biochemical Journal, 294(1), pp. 49-55.

Book Sections

Michie, A.M. , Paunovic, V. and Harnett, M.M. (2018) Cellular homeostasis: Cell growth and cancer. In: Baynes, J. and Dominiczak, M. (eds.) Medical Biochemistry, 5th Edition. Elsevier: New York, NY. ISBN 9780702072994

Middleton, O., Wheadon, H. and Michie, A. (2016) Classical complement pathway. In: Ratcliffe, M.J.H. (ed.) Encyclopedia of Immunobiology, 1st Edition. Elsevier: New York, NY. ISBN 9780123742797

Michie, A.M. , Paunovic, V. and Harnett, M.M. (2014) Cellular homeostasis: cell growth and cancer. In: Baynes, J. and Dominiczak, M. (eds.) Medical Biochemistry, 4th Edition. Elsevier: New York, NY. ISBN 9781455745807

Copland, M. , Michie, A. M. and Holyoake, T. L. (2009) Stem cells in Leukemia and other hematological malignancies. In: Majumder, S. (ed.) Stem Cells and Cancer. Springer New York: New York, NY, pp. 111-136. ISBN 9780387896106 (doi:10.1007/978-0-387-89611-3_5)

Michie, A. M. and Zúñiga-Pflücker, J. C. (1999) Transfection and transcription of genes in developing thymocytes. In: Kearse, K.P. (ed.) T Cell Protocols: Development and Activation. Series: Methods in Molecular Biology, 134. Humana Press, pp. 55-62. ISBN 9780896038103 (doi:10.1385/1-59259-682-7:55)

Michie, A.M. , Harnett, M.M. and Houslay, M.D. (1997) Cyclic nucleotide signalling throughout T cell maturation. In: Harnett, M.M. and Rigley, K.P. (eds.) Lymphocyte Signalling: Mechanism, Subversion and Manipulation. John Wiley & Sons Ltd.: Chichester, p. 247. ISBN 9780471959038

Conference or Workshop Item

Cousins, A. F., Olivares, O., Michie, A. M. , Gottlieb, E. and Halsey, C. (2017) Association of CNS Involvement in Childhood Acute Lymphoblastic Leukaemia with Cholesterol Biosynthesis Upregulation. Spring Meeting for Clinician Scientists in Training 2017, London, UK, 23 Feb 2017. S35. (doi:10.1016/S0140-6736(17)30431-2)

Michie, A.M. , Tarafdar, A., Gallipoli, P., Pellicano, F., Hopcroft, L. , Korfi, K., Cassels, J., Jorgensen, H.J., Vetrie, D. and Holyoake, T.L. (2016) CML Cells Actively Evade Host Immune Surveillance Through Cytokine-Mediated Downregulation of MHC-I I Expression. 36th World Congress of the International Society of Hematology, Glasgow, Scotland, 18-21 Apr 2016. (doi:10.1111/bjh.14019)

This list was generated on Sat Dec 15 19:30:22 2018 GMT.