Dr David I Hughes

  • Senior Lecturer (Centre for Neuroscience)

telephone: 01413304577
email: David.I.Hughes@glasgow.ac.uk

Spinal Cord Group, Room 122, Sir James Black Building, School of Psychology and Neuroscience, University of Glasgow, Glasgow, G12 8QQ

Import to contacts

Further details about the Hughes Group can be found at davehugheslab.org

ORCID iDhttps://orcid.org/0000-0003-1260-3362

Research interests

My research aims to improve our understanding of the complex neurocircuitry in the spinal dorsal horn, an area of critical importance in our ability to perceive the sensations of touch, pain, and itch. In my group, we use a combination of anatomical and electrophysiological approaches in both human and rodent tissue to identify individual cell populations and determine how their activity influences our ability to perceive different sensations.

The main focus of my work has been in collaboration with Dr Brett Graham (University of Newcastle, Australia). We have identified populations of spinal neurons that influence how we perceive the sensations of touch and pain, and established that these cells also play key roles in the development of pathological pain states (tactile allodynia and mechanical hyperalgesia). These circuit mapping studies help us gain insights into how our nervous system processes sensory information and are aimed at identifying novel therapeutic targets for the development more effective treatments to manage chronic pain states.

Key words:
Spinal Cord; Circuit mapping; Anatomy; Electrophysiology; Transgenic mice; AAVs;
Chemogenetics; Optogenetics; Behaviour; Confocal microscopy; Electron microscopy; Chronic pain; Presynaptic inhibition; Postsynaptic inhibition

The Hughes Laboratory has been funded by:
Biotechnology and Biological Sciences Research Council (BBSRC)
Wellcome Trust
National Centre for the Replacement, Refinement and
Reduction of Animals in Research (NC3Rs)
National Health and Medical Research Council (NHMRC; Australia).

Publications

List by: Type | Date

Jump to: 2023 | 2022 | 2021 | 2020 | 2019 | 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2007 | 2005 | 2004 | 2003 | 2002 | 2001 | 2000
Number of items: 50.

2023

Davis, O. C. et al. (2023) Calretinin-expressing islet cells are a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord. Scientific Reports, 13, 11561. (doi: 10.1038/s41598-023-38605-9) (PMID:37464016) (PMCID:PMC10354228)

Boyle, K. A. et al. (2023) Neuropeptide Y-expressing dorsal horn inhibitory interneurons gate spinal pain and itch signalling. eLife, (doi: 10.7554/eLife.86633.1) (Early Online Publication)

2022

Gradwell, M. A., Smith, K. M., Dayas, C. V., Smith, D. W., Hughes, D. I. , Callister, R. J. and Graham, B. A. (2022) Altered intrinsic properties and inhibitory connectivity in aged parvalbumin-expressing dorsal horn neurons. Frontiers in Neural Circuits, 16, 834173. (doi: 10.3389/fncir.2022.834173) (PMID:35874431) (PMCID:PMC9305305)

Hughes, D. I. and Todd, A. J. (2022) Visualizing synaptic connectivity using confocal and electron microscopy: neuroanatomical approaches to define spinal circuits. In: Contemporary Approaches to the Study of Pain. Series: Neuromethods, 178. Humana: New York, pp. 181-201. ISBN 9781071620380 (doi: 10.1007/978-1-0716-2039-7_11)

Barki, N. et al. (2022) Chemogenetics defines the roles of short chain fatty acid receptors within the gut-brain axis. eLife, 11, e73777. (doi: 10.7554/eLife.73777) (PMID:35229717) (PMCID:PMC8887895)

Gradwell, M. A. et al. (2022) Diversity of inhibitory and excitatory parvalbumin interneuron circuits in the dorsal horn. Pain, 163(3), e432-e452. (doi: 10.1097/j.pain.0000000000002422) (PMID:34326298) (PMCID:PMC8832545)

2021

Roesl, C. et al. (2021) Confocal endomicroscopy of neuromuscular junctions stained with physiologically inert protein fragments of tetanus toxin. Biomolecules, 11(10), 1499. (doi: 10.3390/biom11101499) (PMCID:PMC8534034)

Browne, T. J., Smith, K. M., Gradwell, M. A., Iredale, J. A., Dayas, C. V., Callister, R. J., Hughes, D. I. and Graham, B. A. (2021) Spinoparabrachial projection neurons form distinct classes in the mouse dorsal horn. Pain, 162(7), pp. 1977-1994. (doi: 10.1097/j.pain.0000000000002194) (PMID:33779126) (PMCID:PMC8208100)

2020

Browne, T. J., Hughes, D. I. , Dayas, C. V., Callister, R. J. and Graham, B. A. (2020) Projection neuron axon collaterals in the dorsal horn: placing a new player in spinal cord pain processing. Frontiers in Physiology, 11, 560802. (doi: 10.3389/fphys.2020.560802) (PMID:33408637)

Graham, B. A. and Hughes, D. I. (2020) Defining populations of dorsal horn interneurons. Pain, 161(11), pp. 2434-2436. (doi: 10.1097/j.pain.0000000000002067) (PMID:33065697)

Hughes, D. I. and Todd, A. (2020) Central nervous system targets: inhibitory interneurons in the spinal cord. Neurotherapeutics, 17(3), pp. 874-885. (doi: 10.1007/s13311-020-00936-0) (PMID:33029722)

Madden, J.F., Davis, O.C., Boyle, K.A., Iredale, J.A., Browne, T.J., Callister, R.J., Smith, D.W., Jobling, P., Hughes, D.I. and Graham, B.A. (2020) Functional and molecular analysis of proprioceptive sensory neuron excitability in mice. Frontiers in Molecular Neuroscience, 13, 36. (doi: 10.3389/fnmol.2020.00036) (PMID:32477061) (PMCID:PMC7232575)

Browne, T. J., Gradwell, M. A., Iredale, J. A., Madden, J. F., Hughes, D. I. , Callister, R. J., Dayas, C. V. and Graham, B. A. (2020) Transgenic cross-referencing of inhibitory and excitatory interneuron populations to dissect neuronal heterogeneity in the dorsal horn. Frontiers in Molecular Neuroscience, 13, 32. (doi: 10.3389/fnmol.2020.00032) (PMID:32362812) (PMCID:PMC7180513)

2019

Smith, K. M. et al. (2019) Calretinin positive neurons form an excitatory amplifier network in the spinal cord dorsal horn. eLife, 8, e49190. (doi: 10.7554/eLife.49190) (PMID:31713514) (PMCID:PMC6908433)

Graham, B. A. and Hughes, D. I. (2019) Rewards, perils and pitfalls of untangling spinal pain circuits. Current Opinion in Physiology, 11, pp. 35-41. (doi: 10.1016/j.cophys.2019.04.015)

Boyle, K. A. et al. (2019) Defining a spinal microcircuit that gates myelinated afferent input: implications for tactile allodynia. Cell Reports, 28(2), 526-540.e6. (doi: 10.1016/j.celrep.2019.06.040) (PMID:31291586) (PMCID:PMC6635381)

Gutierrez-Mecinas, M. , Davis, O., Polgár, E., Shahzad, M., Navarro-Batista, K., Furuta, T., Watanabe, M., Hughes, D. I. and Todd, A. J. (2019) Expression of calretinin among different neurochemical classes of interneuron in the superficial dorsal horn of the mouse spinal cord. Neuroscience, 398, pp. 171-181. (doi: 10.1016/j.neuroscience.2018.12.009) (PMID:30553791) (PMCID:PMC6347472)

2018

Bautzova, T. et al. (2018) 5-oxoETE triggers nociception in constipation-predominant irritable bowel syndrome through MAS-related G protein–coupled receptor D. Science Signaling, 11(561), eaal2171. (doi: 10.1126/scisignal.aal2171) (PMID:30563864) (PMCID:PMC6411128)

2017

Gradwell, M.A., Boyle, K.A., Callister, R.J., Hughes, D.I. and Graham, B.A. (2017) Heteromeric α/β glycine receptors regulate excitability in parvalbumin-expressing dorsal horn neurons through phasic and tonic glycinergic inhibition. Journal of Physiology, 595(23), pp. 7185-7202. (doi: 10.1113/JP274926) (PMID:28905384) (PMCID:PMC5709328)

Tae, H.-S. et al. (2017) Gabapentin modulates HCN4 channel voltage-dependence. Frontiers in Pharmacology, 8, 554. (doi: 10.3389/fphar.2017.00554) (PMID:28871229) (PMCID:PMC5566583)

Flynn, J. R., Conn, V., Boyle, K., Hughes, D. I. , Watanabe, M., Velasquez, T., Goulding, M. D., Callister, R. J. and Graham, B. A. (2017) Anatomical and molecular properties of long descending propriospinal neurons in mice. Frontiers in Neuroanatomy, 11, 5. (doi: 10.3389/fnana.2017.00005) (PMID:28220062) (PMCID:PMC5292581)

Abraira, V. E. et al. (2017) The cellular and synaptic architecture of the mechanosensory dorsal horn. Cell, 168(1-2), 295-310.e19. (doi: 10.1016/j.cell.2016.12.010) (PMID:28041852) (PMCID:PMC5236062)

2016

Smith, K.M., Boyle, K.A., Mustapa, M., Jobling, P., Callister, R.J., Hughes, D.I. and Graham, B.A. (2016) Distinct forms of synaptic inhibition and neuromodulation regulate calretinin positive neuron excitability in the spinal cord dorsal horn. Neuroscience, 326, pp. 10-21. (doi: 10.1016/j.neuroscience.2016.03.058) (PMID:27045594) (PMCID:PMC4919388)

Iwagaki, N., Ganley, R. P., Dickie, A., Polgár, E., Hughes, D. I. , Del Rio, P., Revina, Y., Watanabe, M., Todd, A. J. and Riddell, J. S. (2016) A combined electrophysiological and morphological study of NPY-expressing inhibitory interneurons in the spinal dorsal horn of the mouse. Pain, 157(3), pp. 598-612. (doi: 10.1097/j.pain.0000000000000407) (PMID:26882346) (PMCID:PMC4751741)

2015

Tadros, M. A., Lim, R., Hughes, D. I. , Brichta, A. M. and Callister, R. J. (2015) Electrical maturation of spinal neurons in the human fetus: comparison of ventral and dorsal horn. Journal of Neurophysiology, 114(5), pp. 2661-2671. (doi: 10.1152/jn.00682.2015) (PMID:26334015)

Smith, K.M., Boyle, K.A., Madden, J.F., Dickinson, S.A., Jobling, P., Callister, R.J., Hughes, D.I. and Graham, B.A. (2015) Functional heterogeneity of calretinin-expressing neurons in the mouse superficial dorsal horn: implications for spinal pain processing. Journal of Physiology, 593(19), pp. 4319-4339. (doi: 10.1113/JP270855) (PMID:26136181) (PMCID:PMC4594251)

2014

Smith, K. M., Madden, J. F., Callister, R. J., Hughes, D. I. and Graham, B. A. (2014) The search for novel analgesics: re-examining spinal cord circuits with new tools. Frontiers in Pharmacology, 5(22), pp. 1-6. (doi: 10.3389/fphar.2014.00022) (PMID:24616699) (PMCID:PMC3934318)

Harris, B. M., Hughes, D. I. , Bolton, P. S., Tadross, M. A., Callister, R. J. and Graham, B. A. (2014) Contrasting alterations to synaptic and intrinsic properties in upper-cervical superficial dorsal horn neurons following acute neck muscle inflammation. Molecular Pain, 10(25), (doi: 10.1186/1744-8069-10-25) (PMID:24725960) (PMCID:PMC4032164)

2013

Hughes, D.I. , Boyle, K., Kinnon, C.M., Bilsland, C., Quayle, J., Callister, R.J. and Graham, B.A. (2013) HCN4 subunit expression in fast-spiking interneurons of the rat spinal cord and hippocampus. Neuroscience, 237, pp. 7-18. (doi: 10.1016/j.neuroscience.2013.01.028) (PMID:23357121) (PMCID:PMC3620460)

Polgár, E., Durrieux, C., Hughes, D.I. and Todd, A.J. (2013) A quantitative study of inhibitory interneurons in laminae I-III of the mouse spinal dorsal horn. PLoS ONE, 8(10), e78309. (doi: 10.1371/journal.pone.0078309) (PMID:24205193) (PMCID:PMC3808353)

2012

Hughes, D.I. , Sikander, S., Kinnon, C.M., Boyle, K.A., Watanabe, M., Callister, R.J. and Graham, B.A. (2012) Morphological, neurochemical and electrophysiological features of parvalbumin-expressing cells: a likely source of axo-axonic inputs in the mouse spinal dorsal horn. Journal of Physiology, 590(16), pp. 3927-3951. (doi: 10.1113/jphysiol.2012.235655)

2011

Shehab, S.A.S. and Hughes, D.I. (2011) Simultaneous identification of unmyelinated and myelinated primary somatic afferents by co-injection of isolectin B4 and Cholera toxin subunit B into the sciatic nerve of the rat. Journal of Neuroscience Methods, 198(2), pp. 213-221. (doi: 10.1016/j.jneumeth.2011.04.002)

2010

Yasaka, T., Tiong, S.Y.X., Hughes, D.I. , Riddell, J.S. and Todd, A.J. (2010) Populations of inhibitory and excitatory interneurons in lamina II of the adult rat spinal dorsal horn revealed by a combined electrophysiological and anatomical approach. Pain, 151(2), pp. 475-488. (doi: 10.1016/j.pain.2010.08.008)

2009

Yasaka, T., Hughes, D.I. , Polgár, E., Nagy, G.G., Watanabe, M., Riddell, J.S. and Todd, A.J. (2009) Evidence against AMPA receptor-lacking glutamatergic synapses in the superficial dorsal horn of the rat spinal cord. Journal of Neuroscience, 29(42), pp. 13401-13409. (doi: 10.1523/JNEUROSCI.2628-09.2009)

2007

Hughes, D.I. , Scott, D.T., Riddell, J.S. and Todd, A.J. (2007) Up-regulation of substance P in low-threshold myelinated afferents is not required for tactile allodynia in the chronic constriction injury and spinal nerve ligation models. Journal of Neuroscience, 27(8), pp. 2035-2044. (doi: 10.1523/JNEUROSCI.5401-06.2007)

Hughes, D.I. , Scott, D.T., Riddell, J.S. and Todd, A.J. (2007) Upregulation of substance P in low-threshold myelinated afferents is not required for tactile allodynia in the chronic constriction injury and spinal nerve ligation models. Journal of Neuroscience, 27(8), pp. 2035-2044. (doi: 10.1523/JNEUROSCI.5401-06.2007)

2005

Shehab, S., McGonigle, D., Hughes, D.I. , Todd, A.J. and Redgrave, P. (2005) Anatomical evidence for an anticonvulsant relay in the rat ventromedial medulla. European Journal of Neuroscience, 22(6), pp. 1431-1444. (doi: 10.1111/j.1460-9568.2005.04326.x)

Polgar, E., Hughes, D.I. , Arham, A.Z. and Todd, A.J. (2005) Loss of neurons from laminas I-III of the spinal dorsal horn is not required for development of tactile allodynia in the spared nerve injury model of neuropathic pain. Journal of Neuroscience, 25(28), pp. 6658-6666. (doi: 10.1523/JNEUROSCI.1490-05.2005)

Hughes, D.I. et al. (2005) P boutons in lamina IX of the rodent spinal cord express high levels of glutamic acid decarboxylase-65 and originate from cells in deep medial dorsal horn. Proceedings of the National Academy of Sciences of the United States of America, 102(25), pp. 9038-9043. (doi: 10.1073/pnas.0503646102)

2004

Hughes, D.I. , Polgár, E., Shehab, S.A.S. and Todd, A.J. (2004) Peripheral axotomy induces depletion of the vesicular glutamate transporter VGLUT1 in central terminals of myelinated afferent fibres in the rat spinal cord. Brain Research, 1017(1-2), pp. 69-76. (doi: 10.1016/j.brainres.2004.05.054)

2003

Hughes, D.I. , Scott, D.T., Todd, A.J. and Riddell, J.S. (2003) Lack of evidence for sprouting of Aβ afferents into the superficial laminas of the spinal cord dorsal horn after nerve section. Journal of Neuroscience, 23(29), pp. 9491-9499.

Polgár, E., Hughes, D.I. , Riddell, J.S. , Maxwell, D.J. , Puskár, Z. and Todd, A.J. (2003) Selective loss of spinal GABAergic or glycinergic neurons is not necessary for development of thermal hyperalgesia in the chronic constriction injury model of neuropathic pain. Pain, 104(1), pp. 229-239. (doi: 10.1016/S0304-3959(03)00011-3)

Todd, A.J. , Hughes, D.I. , Polgár, E., Nagy, G.G., Mackie, M., Ottersen, O.P. and Maxwell, D.J. (2003) The expression of vesicular glutamate transporters VGLUT1 and VGLUT2 in neurochemically defined axonal populations in the rat spinal cord with emphasis on the dorsal horn. European Journal of Neuroscience, 17(1), pp. 13-27. (doi: 10.1046/j.1460-9568.2003.02406.x)

Mackie, M., Hughes, D.I. , Maxwell, D.J. , Tillakaratne, N.J.K. and Todd, A.J. (2003) Distribution and colocalisation of glutamate decarboxylase isoforms in the rat spinal cord. Neuroscience Letters, 119, pp. 461-472. (doi: 10.1016/S0306-4522(03)00174-X)

Pawelzik, H., Hughes, D.I. and Thomson, A.M. (2003) Modulation of inhibitory autapses and synapses on rat CA1 interneurones by GABAa receptor ligands. Journal of Physiology, 546(3), pp. 701-716. (doi: 10.1113/jphysiol.2002.035121)

2002

Watson, A.H.D., Hughes, D.I. and Bazzaz, A.A. (2002) Synaptic relationships between hair follicle afferents and neurones expressing GABA and glycine-like immunoreactivity in the spinal cord of the rat. Journal of Comparative Neurology, 452(4), pp. 367-380. (doi: 10.1002/cne.10410)

Pawelzik, H., Hughes, D.I. and Thomson, A.M. (2002) Physiological and morphological diversity of immunocytochemically defined parvalbumin- and cholecystokinin-positive interneurones in CA1 of the adult rat hippocampus. Journal of Comparative Neurology, 443(4), pp. 346-367. (doi: 10.1002/cne.10118)

2001

Zagon, A. and Hughes, D.I. (2001) Gating of vagal inputs by sciatic afferents in nonspinally projecting neurons in the rat rostral ventrolateral medulla oblongata. Journal of Neuroscience Methods, 13(4), pp. 781-792. (doi: 10.1046/j.0953-816x.2000.01445.x)

2000

Hughes, D.I. , Bannister, A.P., Pawelzik, H. and Thomson, A.M. (2000) Double immunofluorescence, peroxidase labelling and ultrastructural analysis of interneurones following prolonged electrophysiological recordings in vitro. Journal of Neuroscience Methods, 101(2), pp. 107-116. (doi: 10.1016/S0165-0270(00)00254-5)

Thomson, A.M., Bannister, A.P., Hughes, D.I. and Pawelzik, H. (2000) Differential sensitivity to Zolpidem of IPSPs activated by morphologically identified CA1 interneurons in slices of rat hippocampus. European Journal of Neuroscience, 12(2), pp. 425-436. (doi: 10.1046/j.1460-9568.2000.00915.x)

This list was generated on Fri Sep 29 15:02:07 2023 BST.
Jump to: Articles | Book Sections
Number of items: 50.

Articles

Davis, O. C. et al. (2023) Calretinin-expressing islet cells are a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord. Scientific Reports, 13, 11561. (doi: 10.1038/s41598-023-38605-9) (PMID:37464016) (PMCID:PMC10354228)

Boyle, K. A. et al. (2023) Neuropeptide Y-expressing dorsal horn inhibitory interneurons gate spinal pain and itch signalling. eLife, (doi: 10.7554/eLife.86633.1) (Early Online Publication)

Gradwell, M. A., Smith, K. M., Dayas, C. V., Smith, D. W., Hughes, D. I. , Callister, R. J. and Graham, B. A. (2022) Altered intrinsic properties and inhibitory connectivity in aged parvalbumin-expressing dorsal horn neurons. Frontiers in Neural Circuits, 16, 834173. (doi: 10.3389/fncir.2022.834173) (PMID:35874431) (PMCID:PMC9305305)

Barki, N. et al. (2022) Chemogenetics defines the roles of short chain fatty acid receptors within the gut-brain axis. eLife, 11, e73777. (doi: 10.7554/eLife.73777) (PMID:35229717) (PMCID:PMC8887895)

Gradwell, M. A. et al. (2022) Diversity of inhibitory and excitatory parvalbumin interneuron circuits in the dorsal horn. Pain, 163(3), e432-e452. (doi: 10.1097/j.pain.0000000000002422) (PMID:34326298) (PMCID:PMC8832545)

Roesl, C. et al. (2021) Confocal endomicroscopy of neuromuscular junctions stained with physiologically inert protein fragments of tetanus toxin. Biomolecules, 11(10), 1499. (doi: 10.3390/biom11101499) (PMCID:PMC8534034)

Browne, T. J., Smith, K. M., Gradwell, M. A., Iredale, J. A., Dayas, C. V., Callister, R. J., Hughes, D. I. and Graham, B. A. (2021) Spinoparabrachial projection neurons form distinct classes in the mouse dorsal horn. Pain, 162(7), pp. 1977-1994. (doi: 10.1097/j.pain.0000000000002194) (PMID:33779126) (PMCID:PMC8208100)

Browne, T. J., Hughes, D. I. , Dayas, C. V., Callister, R. J. and Graham, B. A. (2020) Projection neuron axon collaterals in the dorsal horn: placing a new player in spinal cord pain processing. Frontiers in Physiology, 11, 560802. (doi: 10.3389/fphys.2020.560802) (PMID:33408637)

Graham, B. A. and Hughes, D. I. (2020) Defining populations of dorsal horn interneurons. Pain, 161(11), pp. 2434-2436. (doi: 10.1097/j.pain.0000000000002067) (PMID:33065697)

Hughes, D. I. and Todd, A. (2020) Central nervous system targets: inhibitory interneurons in the spinal cord. Neurotherapeutics, 17(3), pp. 874-885. (doi: 10.1007/s13311-020-00936-0) (PMID:33029722)

Madden, J.F., Davis, O.C., Boyle, K.A., Iredale, J.A., Browne, T.J., Callister, R.J., Smith, D.W., Jobling, P., Hughes, D.I. and Graham, B.A. (2020) Functional and molecular analysis of proprioceptive sensory neuron excitability in mice. Frontiers in Molecular Neuroscience, 13, 36. (doi: 10.3389/fnmol.2020.00036) (PMID:32477061) (PMCID:PMC7232575)

Browne, T. J., Gradwell, M. A., Iredale, J. A., Madden, J. F., Hughes, D. I. , Callister, R. J., Dayas, C. V. and Graham, B. A. (2020) Transgenic cross-referencing of inhibitory and excitatory interneuron populations to dissect neuronal heterogeneity in the dorsal horn. Frontiers in Molecular Neuroscience, 13, 32. (doi: 10.3389/fnmol.2020.00032) (PMID:32362812) (PMCID:PMC7180513)

Smith, K. M. et al. (2019) Calretinin positive neurons form an excitatory amplifier network in the spinal cord dorsal horn. eLife, 8, e49190. (doi: 10.7554/eLife.49190) (PMID:31713514) (PMCID:PMC6908433)

Graham, B. A. and Hughes, D. I. (2019) Rewards, perils and pitfalls of untangling spinal pain circuits. Current Opinion in Physiology, 11, pp. 35-41. (doi: 10.1016/j.cophys.2019.04.015)

Boyle, K. A. et al. (2019) Defining a spinal microcircuit that gates myelinated afferent input: implications for tactile allodynia. Cell Reports, 28(2), 526-540.e6. (doi: 10.1016/j.celrep.2019.06.040) (PMID:31291586) (PMCID:PMC6635381)

Gutierrez-Mecinas, M. , Davis, O., Polgár, E., Shahzad, M., Navarro-Batista, K., Furuta, T., Watanabe, M., Hughes, D. I. and Todd, A. J. (2019) Expression of calretinin among different neurochemical classes of interneuron in the superficial dorsal horn of the mouse spinal cord. Neuroscience, 398, pp. 171-181. (doi: 10.1016/j.neuroscience.2018.12.009) (PMID:30553791) (PMCID:PMC6347472)

Bautzova, T. et al. (2018) 5-oxoETE triggers nociception in constipation-predominant irritable bowel syndrome through MAS-related G protein–coupled receptor D. Science Signaling, 11(561), eaal2171. (doi: 10.1126/scisignal.aal2171) (PMID:30563864) (PMCID:PMC6411128)

Gradwell, M.A., Boyle, K.A., Callister, R.J., Hughes, D.I. and Graham, B.A. (2017) Heteromeric α/β glycine receptors regulate excitability in parvalbumin-expressing dorsal horn neurons through phasic and tonic glycinergic inhibition. Journal of Physiology, 595(23), pp. 7185-7202. (doi: 10.1113/JP274926) (PMID:28905384) (PMCID:PMC5709328)

Tae, H.-S. et al. (2017) Gabapentin modulates HCN4 channel voltage-dependence. Frontiers in Pharmacology, 8, 554. (doi: 10.3389/fphar.2017.00554) (PMID:28871229) (PMCID:PMC5566583)

Flynn, J. R., Conn, V., Boyle, K., Hughes, D. I. , Watanabe, M., Velasquez, T., Goulding, M. D., Callister, R. J. and Graham, B. A. (2017) Anatomical and molecular properties of long descending propriospinal neurons in mice. Frontiers in Neuroanatomy, 11, 5. (doi: 10.3389/fnana.2017.00005) (PMID:28220062) (PMCID:PMC5292581)

Abraira, V. E. et al. (2017) The cellular and synaptic architecture of the mechanosensory dorsal horn. Cell, 168(1-2), 295-310.e19. (doi: 10.1016/j.cell.2016.12.010) (PMID:28041852) (PMCID:PMC5236062)

Smith, K.M., Boyle, K.A., Mustapa, M., Jobling, P., Callister, R.J., Hughes, D.I. and Graham, B.A. (2016) Distinct forms of synaptic inhibition and neuromodulation regulate calretinin positive neuron excitability in the spinal cord dorsal horn. Neuroscience, 326, pp. 10-21. (doi: 10.1016/j.neuroscience.2016.03.058) (PMID:27045594) (PMCID:PMC4919388)

Iwagaki, N., Ganley, R. P., Dickie, A., Polgár, E., Hughes, D. I. , Del Rio, P., Revina, Y., Watanabe, M., Todd, A. J. and Riddell, J. S. (2016) A combined electrophysiological and morphological study of NPY-expressing inhibitory interneurons in the spinal dorsal horn of the mouse. Pain, 157(3), pp. 598-612. (doi: 10.1097/j.pain.0000000000000407) (PMID:26882346) (PMCID:PMC4751741)

Tadros, M. A., Lim, R., Hughes, D. I. , Brichta, A. M. and Callister, R. J. (2015) Electrical maturation of spinal neurons in the human fetus: comparison of ventral and dorsal horn. Journal of Neurophysiology, 114(5), pp. 2661-2671. (doi: 10.1152/jn.00682.2015) (PMID:26334015)

Smith, K.M., Boyle, K.A., Madden, J.F., Dickinson, S.A., Jobling, P., Callister, R.J., Hughes, D.I. and Graham, B.A. (2015) Functional heterogeneity of calretinin-expressing neurons in the mouse superficial dorsal horn: implications for spinal pain processing. Journal of Physiology, 593(19), pp. 4319-4339. (doi: 10.1113/JP270855) (PMID:26136181) (PMCID:PMC4594251)

Smith, K. M., Madden, J. F., Callister, R. J., Hughes, D. I. and Graham, B. A. (2014) The search for novel analgesics: re-examining spinal cord circuits with new tools. Frontiers in Pharmacology, 5(22), pp. 1-6. (doi: 10.3389/fphar.2014.00022) (PMID:24616699) (PMCID:PMC3934318)

Harris, B. M., Hughes, D. I. , Bolton, P. S., Tadross, M. A., Callister, R. J. and Graham, B. A. (2014) Contrasting alterations to synaptic and intrinsic properties in upper-cervical superficial dorsal horn neurons following acute neck muscle inflammation. Molecular Pain, 10(25), (doi: 10.1186/1744-8069-10-25) (PMID:24725960) (PMCID:PMC4032164)

Hughes, D.I. , Boyle, K., Kinnon, C.M., Bilsland, C., Quayle, J., Callister, R.J. and Graham, B.A. (2013) HCN4 subunit expression in fast-spiking interneurons of the rat spinal cord and hippocampus. Neuroscience, 237, pp. 7-18. (doi: 10.1016/j.neuroscience.2013.01.028) (PMID:23357121) (PMCID:PMC3620460)

Polgár, E., Durrieux, C., Hughes, D.I. and Todd, A.J. (2013) A quantitative study of inhibitory interneurons in laminae I-III of the mouse spinal dorsal horn. PLoS ONE, 8(10), e78309. (doi: 10.1371/journal.pone.0078309) (PMID:24205193) (PMCID:PMC3808353)

Hughes, D.I. , Sikander, S., Kinnon, C.M., Boyle, K.A., Watanabe, M., Callister, R.J. and Graham, B.A. (2012) Morphological, neurochemical and electrophysiological features of parvalbumin-expressing cells: a likely source of axo-axonic inputs in the mouse spinal dorsal horn. Journal of Physiology, 590(16), pp. 3927-3951. (doi: 10.1113/jphysiol.2012.235655)

Shehab, S.A.S. and Hughes, D.I. (2011) Simultaneous identification of unmyelinated and myelinated primary somatic afferents by co-injection of isolectin B4 and Cholera toxin subunit B into the sciatic nerve of the rat. Journal of Neuroscience Methods, 198(2), pp. 213-221. (doi: 10.1016/j.jneumeth.2011.04.002)

Yasaka, T., Tiong, S.Y.X., Hughes, D.I. , Riddell, J.S. and Todd, A.J. (2010) Populations of inhibitory and excitatory interneurons in lamina II of the adult rat spinal dorsal horn revealed by a combined electrophysiological and anatomical approach. Pain, 151(2), pp. 475-488. (doi: 10.1016/j.pain.2010.08.008)

Yasaka, T., Hughes, D.I. , Polgár, E., Nagy, G.G., Watanabe, M., Riddell, J.S. and Todd, A.J. (2009) Evidence against AMPA receptor-lacking glutamatergic synapses in the superficial dorsal horn of the rat spinal cord. Journal of Neuroscience, 29(42), pp. 13401-13409. (doi: 10.1523/JNEUROSCI.2628-09.2009)

Hughes, D.I. , Scott, D.T., Riddell, J.S. and Todd, A.J. (2007) Up-regulation of substance P in low-threshold myelinated afferents is not required for tactile allodynia in the chronic constriction injury and spinal nerve ligation models. Journal of Neuroscience, 27(8), pp. 2035-2044. (doi: 10.1523/JNEUROSCI.5401-06.2007)

Hughes, D.I. , Scott, D.T., Riddell, J.S. and Todd, A.J. (2007) Upregulation of substance P in low-threshold myelinated afferents is not required for tactile allodynia in the chronic constriction injury and spinal nerve ligation models. Journal of Neuroscience, 27(8), pp. 2035-2044. (doi: 10.1523/JNEUROSCI.5401-06.2007)

Shehab, S., McGonigle, D., Hughes, D.I. , Todd, A.J. and Redgrave, P. (2005) Anatomical evidence for an anticonvulsant relay in the rat ventromedial medulla. European Journal of Neuroscience, 22(6), pp. 1431-1444. (doi: 10.1111/j.1460-9568.2005.04326.x)

Polgar, E., Hughes, D.I. , Arham, A.Z. and Todd, A.J. (2005) Loss of neurons from laminas I-III of the spinal dorsal horn is not required for development of tactile allodynia in the spared nerve injury model of neuropathic pain. Journal of Neuroscience, 25(28), pp. 6658-6666. (doi: 10.1523/JNEUROSCI.1490-05.2005)

Hughes, D.I. et al. (2005) P boutons in lamina IX of the rodent spinal cord express high levels of glutamic acid decarboxylase-65 and originate from cells in deep medial dorsal horn. Proceedings of the National Academy of Sciences of the United States of America, 102(25), pp. 9038-9043. (doi: 10.1073/pnas.0503646102)

Hughes, D.I. , Polgár, E., Shehab, S.A.S. and Todd, A.J. (2004) Peripheral axotomy induces depletion of the vesicular glutamate transporter VGLUT1 in central terminals of myelinated afferent fibres in the rat spinal cord. Brain Research, 1017(1-2), pp. 69-76. (doi: 10.1016/j.brainres.2004.05.054)

Hughes, D.I. , Scott, D.T., Todd, A.J. and Riddell, J.S. (2003) Lack of evidence for sprouting of Aβ afferents into the superficial laminas of the spinal cord dorsal horn after nerve section. Journal of Neuroscience, 23(29), pp. 9491-9499.

Polgár, E., Hughes, D.I. , Riddell, J.S. , Maxwell, D.J. , Puskár, Z. and Todd, A.J. (2003) Selective loss of spinal GABAergic or glycinergic neurons is not necessary for development of thermal hyperalgesia in the chronic constriction injury model of neuropathic pain. Pain, 104(1), pp. 229-239. (doi: 10.1016/S0304-3959(03)00011-3)

Todd, A.J. , Hughes, D.I. , Polgár, E., Nagy, G.G., Mackie, M., Ottersen, O.P. and Maxwell, D.J. (2003) The expression of vesicular glutamate transporters VGLUT1 and VGLUT2 in neurochemically defined axonal populations in the rat spinal cord with emphasis on the dorsal horn. European Journal of Neuroscience, 17(1), pp. 13-27. (doi: 10.1046/j.1460-9568.2003.02406.x)

Mackie, M., Hughes, D.I. , Maxwell, D.J. , Tillakaratne, N.J.K. and Todd, A.J. (2003) Distribution and colocalisation of glutamate decarboxylase isoforms in the rat spinal cord. Neuroscience Letters, 119, pp. 461-472. (doi: 10.1016/S0306-4522(03)00174-X)

Pawelzik, H., Hughes, D.I. and Thomson, A.M. (2003) Modulation of inhibitory autapses and synapses on rat CA1 interneurones by GABAa receptor ligands. Journal of Physiology, 546(3), pp. 701-716. (doi: 10.1113/jphysiol.2002.035121)

Watson, A.H.D., Hughes, D.I. and Bazzaz, A.A. (2002) Synaptic relationships between hair follicle afferents and neurones expressing GABA and glycine-like immunoreactivity in the spinal cord of the rat. Journal of Comparative Neurology, 452(4), pp. 367-380. (doi: 10.1002/cne.10410)

Pawelzik, H., Hughes, D.I. and Thomson, A.M. (2002) Physiological and morphological diversity of immunocytochemically defined parvalbumin- and cholecystokinin-positive interneurones in CA1 of the adult rat hippocampus. Journal of Comparative Neurology, 443(4), pp. 346-367. (doi: 10.1002/cne.10118)

Zagon, A. and Hughes, D.I. (2001) Gating of vagal inputs by sciatic afferents in nonspinally projecting neurons in the rat rostral ventrolateral medulla oblongata. Journal of Neuroscience Methods, 13(4), pp. 781-792. (doi: 10.1046/j.0953-816x.2000.01445.x)

Hughes, D.I. , Bannister, A.P., Pawelzik, H. and Thomson, A.M. (2000) Double immunofluorescence, peroxidase labelling and ultrastructural analysis of interneurones following prolonged electrophysiological recordings in vitro. Journal of Neuroscience Methods, 101(2), pp. 107-116. (doi: 10.1016/S0165-0270(00)00254-5)

Thomson, A.M., Bannister, A.P., Hughes, D.I. and Pawelzik, H. (2000) Differential sensitivity to Zolpidem of IPSPs activated by morphologically identified CA1 interneurons in slices of rat hippocampus. European Journal of Neuroscience, 12(2), pp. 425-436. (doi: 10.1046/j.1460-9568.2000.00915.x)

Book Sections

Hughes, D. I. and Todd, A. J. (2022) Visualizing synaptic connectivity using confocal and electron microscopy: neuroanatomical approaches to define spinal circuits. In: Contemporary Approaches to the Study of Pain. Series: Neuromethods, 178. Humana: New York, pp. 181-201. ISBN 9781071620380 (doi: 10.1007/978-1-0716-2039-7_11)

This list was generated on Fri Sep 29 15:02:07 2023 BST.

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Spinal modulation of non-peptidergic C-nociceptor input by inhibitory calretinin neurons
    Biotechnology and Biological Sciences Research Council
    2023 - 2025
     
  • ENDOSCOP: Establishing Neuronal Drivers and the Spinal Circuitry of Osteoarthritis Pain
    Wellcome Trust
    2023 - 2031
     
  • Projection neuron axon collaterals in the dorsal horn: the missing link in spinal pain processing?
    National Health and Medical Research Council
    2020 - 2023
     
  • Excitatory interneurons: a sensory amplifier for pathological pain
    National Health and Medical Research Council
    2018 - 2020
     
  • Determining the role of calretinin-RorB spinal interneurons in modulating mechanical pain
    Biotechnology and Biological Sciences Research Council
    2017 - 2020
     
  • Spinal processing of sensory signals from the gut
    National Health and Medical Research Council
    2014 - 2016
     
  • CRACK IT Challenge 9: DRGNET: Enabling access to primary human dorsal root ganglion neurones for drug target identification and pharmacological experimentation
    National Centre for the Replacement of Animals Research
    2014 - 2018
     
  • CRACK IT Challenge 9: DRGNET: Enabling access to primary human dorsal root ganglion neurones for drug target identification and pharmacological experimentation
    National Centre for the Replacement of Animals Research
    2013 - 2014
     
  • The role of presynaptic inhibition in neuropathic pain
    National Health and Medical Research Council
    2013 - 2015
     
  • Development of peripheral sensory pathways in humans
    National Health and Medical Research Council
    2012 - 2016
     
  • Modulating cutaneous afferent input: Identifying a source of presynaptic (axo-axonic) inputs in the mouse spinal dorsal horn
    Biotechnology and Biological Sciences Research Council
    2012 - 2015
     

Supervision

Supervised Postgraduate Students

  • Wafa Abdulsalam Alsulaiman (Postgraduate)
  • Fares Aboushnaf (Postgraduate)

Teaching

Teaching Administration

Degree Programme Coordination

Anatomy BSc Honours (Final Year): Coordinator 2010-2016, Deputy Coordinator 2009-2010, 2016 - present
Anatomy Junior Honours (Year 3): Coordinator 2009-2012, Deputy Coordinator 2012-2016
Anatomy Examinations Officer 2011-2016

Degree Course Coordination

Course leader: “Advanced Neuroanatomy” for BSc honours students; 2011 - present
Course leader: “Anatomy Advanced Studies” for BSc honours students in Anatomy; 2011 - 2016
Course leader: “Skills for the Modern Professional” for intercalating medical students; 2011 – 2016

 

Teaching: Delivery and Assessment

Undergraduate teaching

Year 3: Life Science courses in Anatomy, Neuroscience, Physiology and Pharmacology.
Years 4 and 5: Human Biology courses in “Autonomic Nervous System”, “Developmental Neuroscience”, “Advanced Neuroanatomy”, and “Bio-imaging in Life Science”.

Professional courses

Year 1:  Medical students (MBChB1):      Limbs and Back course
Year 1:  Dental students (BDS1):              Biological and Medical Sciences
Year 2:  Medical students (MBChB2):      Head, Neck and Neurological Systems

Postgraduate teaching

MSc Brain Sciences
MSc Biomedical Sciences
MSc BioImaging
MSc in vitro and Analytical Approaches in Neuroscience
Summer School: in vivo skills in Neuroscience

 

Student Mentoring and Supervision

Advisor of Studies, School of Life Sciences: 2012 – present
Research mentor to postdoctoral researchers: 2012 - present

I supervise at least four undergraduate (BSc honours) project students each academic year, and up to three postgraduate (Masters) students from the Brain Sciences or Biomedical Sciences courses. I also supervise summer projects.

 

PhD Students

Marami Binti Mustapa
Andrew Bell

Additional information

Invited International Presentations

  • 2016: Yokohama, Japan - IASP Workshop Organiser: Inhibitory Interneurons in the Spinal Dorsal Horn: Recent Advances
  • 2016: Yokohama, Japan - Invited Speaker; IASP’s 16th World Congress on Pain
  • 2016: Taormina, Sicily, Italy - Invited Speaker; Pain Mechanisms and Therapeutics Conference
  • 2015: Queen Mary University of London - Invited Speaker; Neurogastroenterology Group, Queen Mary University of London
  • 2015: Janelia Research Campus, USA - Invited Speaker; 'Mammalian Circuits Underlying Somatosensation', Howard Hughes Medical Institute, Janelia Research Campus, USA.
  • 2013: Galway, Ireland - Invited Speaker; Centre for Pain Research, School of Medicine, NUI Galway
  • 2012: Milan, Italy - IASP Workshop Co-organiser: Dissecting interneuron diversity on the spinal dorsal horn using transgenic mice
  • 2012: Melbourne, Australia - Invited Speaker; Department of Anatomy and Neuroscience, University of Melbourne
  • 2012: Sydney, Australia - Invited Speaker; Sensory Neuroscience Symposium, University of Western Sydney
  • 2012: Milan, Italy - Invited Speaker; IASP's 14th World Congress of Pain
  • 2010: Newcastle, New South Wales, Australia - Invited Speaker; School of Biomedical Sciences and Pharmacy, University of Newcastle
  • 2010: Glasgow, Scotland - Invited Speaker; Scottish Neuroscience Meeting, University of Strathclyde

Professional Learned Society

  • 2017 - present: Frontiers in Neural Circuits - Review Editor
  • 2015 - present: Frontiers in Neuropharmacology - Review Editor
  • 2015 - present: Anatomical Society of Great Britain and Ireland - Fellow
  • 2015 - present: Athena SWAN Institute SAT member
  • 2015 - present: Neuropharmacology - Review Editor
  • 2014 - present: STEMM Ambassador
  • 2008 - present: Anatomical Society of Great Britain and Ireland - Member
  • 2008 - present: International Association for the Study of Pain - Member
  • 1998 - present: Society for Neuroscience - Member

Dr David I Hughes