ID7000 Spectral Analyser
We have moved into the realm of spectral cytometry!
Our newest analyser, the Sony ID7000 is a 5-laser instrument (355nm, 405nm, 488nm, 561nm, 660nm) with 147 detectors. It is different to our conventional cytometers as it doesn’t use physical filter sets (so we don’t have a traditional configuration document) but rather it takes all of the light emitted and unmixes the spectral signatures of individual fluorochromes.
Whilst this is a different way of working the instrument has intuitive software for experiment setup, spectral unmixing and autofluorescence extraction.
The analysis software is free to all users of the system but runs on a PC and requires 16GB of RAM.
Acquired data must be unmixed at the instrument, but thereafter can be analysed off line.
As we look in more detail at our cells, the number of parameters needed increases, and with the proper advice and experimental design, panels of over 40 colours are now possible.
The instrument has an autosampler and walk away analysis is truly possible once settings have been optimised
Please contact flow staff to have your name added to the list of users on the waiting list to be trained.
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