BD LSR FORTESSA ANALYSER
Temporarily reloated to B219 of the GBRC
We have a SORP BD LSR Fortessa flow cytometer. This machine has five lasers: Blue (488mn), Red (640nm), Violet (405nm), Yellow Green (561nm) and UltraViolet (305nm). It can detect 20 parameters simultaneously (18 colours plus forward scatter and side scatter).
Digital pulse sampling allows the measurement of Height, Area and Width from all parameters simultaneously.
Data is acquired through BD FACSDiVaTM digital software.
Typical operation at 20,000 events/ second which requires a sample concentration of 2 x 107 cells/mL at an instrument flow rate of 60 μL/min.
The LSR Fortessa also has a High Throughput Option for analysis. This provides rapid, fully automated sample acquisition from microtiter plates. The HTS option supports a wide range of research applications and is compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates. In high-throughput mode, the HTS can process a 96-well plate in fewer than 15 minutes with less than 1 per cent carryover. Standard throughput mode can be selected to acquire larger sample volumes or where longer acquisition times are required.
The beauty of this machine is that filter and mirror changes can easily be made to customise the optical array for non standard experiments.
For access to this machine please contact the Flow Core Facility Manager.
Applications: Analysis of cells, parasites, bacteria, calcium flux, phospho flow, DNA analysis/cell cycle, apoptosis and multi colour immunophenotyping, Fluorescent proteins, cell proliferation etc.
Follow the link for the base configuration for this machine: LSRFortessa Base Configuration
