Dr Lorraine Work

  • Reader (Cardiovascular & Metabolic Health)

telephone: 01413305869/4962
email: Lorraine.Work@glasgow.ac.uk
pronouns: She/her/hers

C443, SCMH, BHF GCRC, Glasgow G12 8TA

Import to contacts

ORCID iDhttps://orcid.org/0000-0002-6462-4109

Biography

Lorraine Work graduated from the University of Strathclyde with a 1st class BSc (Hons) in Immunology & Pharmacology in 1995 and went on to study for a PhD investigating anti-proliferative agents for therapy in in vitro and in vivo models of vascular injury, graduating in 1999. Following a brief period spent at the University of Washington, Seattle, Dr Lorraine Work joined the Division of Cardiovascular and Medical Science, University of Glasgow in 2000 as a post-doctoral researcher in Professor A. Baker’s research group. In so doing she moved from pharmacological interventions to gene-based approaches - specifically, identifying novel targets which could be used to direct therapy to the vasculature (smooth muscle cells and organ specific endothelial beds). Lorraine was awarded a BHF Intermediate Research Fellowship in 2006 to study a combination of pharmacological and gene-based therapies in stroke. In 2007, Dr Work was appointed lecturer through the BBSRC Capacity Building awards in Integrative Mammalian Biology and so central to her research is the translation of knowledge gained from single molecules back to the whole organism, including in vivo studies. In 2015 Lorraine was promoted to Senior Lecturer and then to Reader in 2019.

Lorraine is Athena Swan Champion for SCMH and represents the school on the College EDI committee. She served on the Athena Swan self-assessment team for several years before becoming chair in 2021. Her support for early career researchers and women in science was recognised by a research culture award (2021)

Dr Lorraine Work - Good Practice in Research Culture

Lorraine also dedicates much of her time to public engagement and outreach activities to communicate the ongoing research interests of her group to the general public and to promote science in schools. Through a collaboration with Dr Lizzie Burns and Professor Stuart Allan (University of Manchester) a series of “build-a-brain” workshops were held in schools across Glasgow. This, alongside a “Brain Health Awareness Day” led to the team being awarded an ICCA Incredible Impacts Award.

ICCA Incredible Impacts Award - 2022 Winners

Working alongside Professors Jason Gill and Godfrey Smith, a new “Have a Heart” workshop has been developed in SCMH and will be delivered to schools as part of an ongoing program of science promotion. In addition, Lorraine has spoken at Pint of Science (2022) and often takes part in tours of supporters of charities which fund her work, such as the British Heart Foundation. Her commitment to engagement activities was recognised in Lorraine being “Highly Commended” researcher in the MVLS Engagement Awards (2022) 

Research interests

Research Theme: Vascular Diseases and Comorbidities

Lorraine’s research interest lies in targeting multiple pathways through both combining gene and drug intervention strategies or using microRNAs in an attempt to reduce infarct volume and improve neurological recovery following experimental stroke. She collaborates closely with clinical colleagues to ensure a good bench to bedside to bench iterative research program.

Increasingly, Dr Work is interested in exploiting endogenous signalling particles, extracellular vesicles as a means of delivery. These small particles carry cargo around the body in health and disease and determining their contents serve both as a potential biomarker but also as a novel therapeutic. Other interests include determining the effect of stabilisation of mitochondrial function on outcome following stroke and how endogenous mitochondrial turnover processes are altered with ischaemic injury and targeting mitochondria therapeutically. Oedema is a contributory pathological mechanism early after ischaemic stroke and collaborative studies are determining if targeting oedema while promoting neuronal plasticity will be more beneficial that targeting each pathway alone. She also has ongoing projects determining the peripheral effects of cerebral ischaemic injury. Her experimental stroke studies consider the impact of intervention on measures of cognition given the key link between stroke and dementia.

Although stroke is the 3rd leading cause of death and the leading cause of long-term disability worldwide there is, currently, only two clinically approved interventions both of which promote reperfusion. In determining the relative contribution of the underlying signalling pathways to infarct size and consequent neurological recovery the Work group hope to identify novel therapeutic approaches. Furthermore, many target microRNAs may be involved in promoting endogenous repair allowing delayed delivery thus promoting clinical translation. The research group use in vitro methods to determine efficacy prior to in vivo application in a model of transient cerebral ischaemia.

Research groups

Publications

List by: Type | Date

Jump to: 2024 | 2023 | 2022 | 2021 | 2020 | 2019 | 2018 | 2016 | 2015 | 2014 | 2013 | 2012 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001 | 1998
Number of items: 47.

2024

Alghanimy, A., Work, L. M. and Holmes, W. M. (2024) The glymphatic system and multiple sclerosis: An evolving connection. Multiple Sclerosis and Related Disorders, 83, 105456. (doi: 10.1016/j.msard.2024.105456)

McFall, A. , Graham, D. , Nicklin, S. A. and Work, L. M. (2024) Unscheduled changes in pre-clinical stroke model housing contributes to variance in physiological and behavioural data outcomes- a post-hoc analysis. Brain and Neuroscience Advances, (Accepted for Publication)

2023

Freeman-Jones, E., Miller, W. H. , Work, L. M. and Fullerton, J. L. (2023) Polypathologies and animal models of traumatic brain injury. Brain Sciences, 13(12), 1709. (doi: 10.3390/brainsci13121709) (PMID:38137157) (PMCID:PMC10741988)

Lyon, M., Fullerton, J. L. , Kennedy, S. and Work, L. M. (2023) Hypertension & dementia: pathophysiology & potential utility of antihypertensives in reducing disease burden. Pharmacology and Therapeutics, (doi: 10.1016/j.pharmthera.2023.108575) (PMID:38052309) (In Press)

Sri, S. et al. (2023) A multi-disciplinary commentary on preclinical research to investigate vascular contributions to dementia. Cerebral Circulation - Cognition and Behavior, 5, 100189. (doi: 10.1016/j.cccb.2023.100189) (PMID:37941765) (PMCID:PMC10628644)

2022

Fullerton, J. L. , Thomas, J. M., Gonzalez-Trueba, L., Trivett, C., Van Kralingen, J. C., Allan, S. M., Quinn, T. J. and Work, L. M. (2022) Systematic review: association between circulating microRNA expression & stroke. Journal of Cerebral Blood Flow and Metabolism, 42(6), pp. 935-951. (doi: 10.1177/0271678X221085090) (PMID:35240874) (PMCID:PMC9121526)

Fullerton, J. L. , Cosgrove, C. C., Rooney, R. A. and Work, L. (2022) Extracellular vesicles and their microRNA cargo in ischaemic stroke. Journal of Physiology, (doi: 10.1113/JP282050) (PMID:35421904) (Early Online Publication)

2021

Alshuhri, M. S. , Gallagher, L., Work, L. and Holmes, W. (2021) Direct imaging of glymphatic flow using H217O MRI. JCI Insight, 6(10), e141159. (doi: 10.1172/jci.insight.141159) (PMID:33857020)

2020

McFall, A. , Nicklin, S. A. and Work, L. M. (2020) The counter regulatory axis of the renin angiotensin system in the brain and ischaemic stroke: insight from preclinical stroke studies and therapeutic potential. Cellular Signalling, 76, 109809. (doi: 10.1016/j.cellsig.2020.109809) (PMID:33059037) (PMCID:PMC7550360)

Pashova, A., Work, L.M. and Nicklin, S.A. (2020) The role of extracellular vesicles in neointima formation post vascular injury. Cellular Signalling, 76, 109783. (doi: 10.1016/j.cellsig.2020.109783) (PMID:32956789)

McFall, A. et al. (2020) UK consensus on pre-clinical vascular cognitive impairment functional outcomes assessment: questionnaire and workshop proceedings. Journal of Cerebral Blood Flow and Metabolism, 40(7), pp. 1402-1414. (doi: 10.1177/0271678X20910552) (PMID:32151228) (PMCID:PMC7307003)

2019

Deuchar, G. A., Van Kralingen, J. C., Work, L. M. , Santosh, C., Muir, K. W. , McCabe, C. and Macrae, I. M. (2019) Preclinical validation of the therapeutic potential of Glasgow Oxygen Level Dependent (GOLD) technology: A theranostic for acute stroke. Translational Stroke Research, 10(5), pp. 583-595. (doi: 10.1007/s12975-018-0679-y) (PMID:30506268)

van Kralingen, J., McFall, A. , Ord, E. N.J. , Coyle, T. F., Bissett, M., McClure, J. D. , McCabe, C. , Macrae, I. M., Dawson, J. and Work, L. M. (2019) Altered extracellular vesicle microrna expression in ischemic stroke and small vessel disease. Translational Stroke Research, 10(5), pp. 495-508. (doi: 10.1007/s12975-018-0682-3) (PMID:30617992) (PMCID:PMC6733813)

Arroja, M.M.C., Reid, E. , Roy, L.A., Vallatos, A.V. , Holmes, W.M. , Nicklin, S.A. , Work, L.M. and McCabe, C. (2019) Assessing the effects of Ang-(1-7) therapy following transient middle cerebral artery occlusion. Scientific Reports, 9, 3154. (doi: 10.1038/s41598-019-39102-8) (PMID:30816157) (PMCID:PMC6395816)

McFall, A. , Dawson, J. and Work, L. M. (2019) Stroke. In: Touyz, R. M. and Delles, C. (eds.) Textbook of Vascular Medicine. Springer: Cham, pp. 461-472. ISBN 9783030164805 (doi: 10.1007/978-3-030-16481-2_43)

2018

Hietamies, T. M., Ostrowski, C., Pei, Z., Feng, L., McCabe, C., Work, L. M. and Quinn, T. J. (2018) Variability of functional outcome measures used in animal models of stroke and vascular cognitive impairment – a review of contemporary studies. Journal of Cerebral Blood Flow and Metabolism, 38(11), pp. 1872-1884. (doi: 10.1177/0271678X18799858) (PMID:30203705)

Horsburgh, K. et al. (2018) Small vessels, dementia and chronic diseases – molecular mechanisms and pathophysiology. Clinical Science, 132(8), pp. 851-868. (doi: 10.1042/CS20171620) (PMID:29712883)

2016

Chouchani, E. T., Pell, V. R., James, A. M., Work, L. M. , Saeb-Parsy, K., Frezza, C., Krieg, T. and Murphy, M. P. (2016) A unifying mechanism for mitochondrial superoxide production during ischemia-reperfusion injury. Cell Metabolism, 23(2), pp. 254-263. (doi: 10.1016/j.cmet.2015.12.009) (PMID:26777689)

2015

Akehurst, C. et al. (2015) Differential expression of microRNA-206 and its target genes in pre-eclampsia. Journal of Hypertension, 33(10), pp. 2068-2074. (doi: 10.1097/HJH.0000000000000656) (PMID:26213997) (PMCID:PMC4570688)

2014

Chouchani, E. T. et al. (2014) Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS. Nature, 515(7527), pp. 431-435. (doi: 10.1038/nature13909) (PMID:25383517) (PMCID:PMC4255242)

Shirley, R., Ord, E. N.J. and Work, L. M. (2014) Oxidative stress and the use of antioxidants in stroke. Antioxidants, 3(3), pp. 472-501. (doi: 10.3390/antiox3030472)

2013

Ord, E. N.J. , Shirley, R., McClure, J. D. , McCabe, C. , Kremer, E. J., Macrae, I. M. and Work, L. M. (2013) Combined antiapoptotic and antioxidant approach to acute neuroprotection for stroke in hypertensive rats. Journal of Cerebral Blood Flow and Metabolism, 33(8), pp. 1215-1224. (doi: 10.1038/jcbfm.2013.70) (PMID:23632970) (PMCID:PMC3734772)

2012

Ord, E. N.J. , Shirley, R., van Kralingen, J. C., Graves, A., McClure, J. D. , Wilkinson, M., McCabe, C. , Macrae, I. M. and Work, L. M. (2012) Positive impact of pre-stroke surgery on survival following transient focal ischemia in hypertensive rats. Journal of Neuroscience Methods, 211(2), pp. 305-308. (doi: 10.1016/j.jneumeth.2012.09.001)

Flores, M., Work, L.M. , Douglas, K., Denby, L., Dominiczak, A.F. , Graham, D. and Nicklin, S.A. (2012) Angiotensin-(1-9) attenuates cardiac fibrosis in the stroke-prone spontaneously hypertensive rat via the angiotensin type 2 receptor. Hypertension, 59(2), pp. 300-307. (doi: 10.1161/HYPERTENSIONAHA.111.177485)

2010

Greig, J.A., Shirley, R., Graham, D. , Denby, L., Dominiczak, A.F. , Work, L.M. and Baker, A. (2010) Vascular-targeting anti-oxidant therapy in a model of hypertension and stroke. Journal of Cardiovascular Pharmacology, 56(6), pp. 642-650. (doi: 10.1097/FJC.0b013e3181f8f19f)

2009

Nicol, C. G., Denby, L., Lopez-Franco, O., Masson, R., Halliday, C. A., Nicklin, S. A., Kritz, A., Work, L. M. and Baker, A. H. (2009) Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature. FEBS Letters, 583(12), pp. 2100-2107. (doi: 10.1016/j.febslet.2009.05.037)

Work, L.M. , Dominiczak, A.F. and McBride, M.W. (2009) The genetic basis of stroke: animal models. In: 3rd Report on Stroke: Genetic research in prevention and in cure. Il Pensiero Scientifico Editore, pp. 219-40.

2008

Graham, T., McIntosh, J., Work, L. , Nathwani, A. and Baker, A. (2008) Performance of AAV8 vectors expressing human factor IX from a hepatic-selective promoter following intravenous injection into rats. Genetic Vaccines and Therapy, 6(1), p. 9. (doi: 10.1186/1479-0556-6-9)

White, K. et al. (2008) Engineering adeno-associated virus 2 vectors for targeted gene delivery to atherosclerotic lesions. Gene Therapy, 15(6), pp. 443-451. (doi: 10.1038/sj.gt.3303077)

2007

Kritz, A. B., Nicol, C. G., Dishart, K. L., Nelson, R., Holbeck, S., Von Seggern, D. J., Work, L. M., Mcvey, J. H., Nicklin, S. A. and Baker, A. H. (2007) Adenovirus 5 fibers mutated at the putative HSPG-binding site show restricted retargeting with targeting peptides in the HI loop. Molecular Therapy, 15, pp. 741-749. (doi: 10.1038/sj.mt.6300094)

Baker, A.H. et al. (2007) Brain protection using autologous bone marrow cell, metalloproteinase inhibitors, and metabolic treatment in cerebral ischemia. Proceedings of the National Academy of Sciences of the United States of America, 104, pp. 3597-3602. (doi: 10.1073/pnas.0611112104)

Denby, L., Work, L.M., Von Seggern, D.J., Wu, E., Mcvey, J.H., Nicklin, S.A. and Baker, A.H. (2007) Development of renal-targeted vectors through combined in vivo phage display and capsid engineering of adenoviral fibers from serotype 19p. Molecular Therapy, 15(9), pp. 1647-1654. (doi: 10.1038/sj.mt.6300214)

2006

Perabo, L. et al. (2006) Heparan sulfate proteoglycan binding properties of adeno-associated virus retargeting mutants and consequences for their in vivo tropism. Journal of Virology, 80(14), pp. 7265-7269. (doi: 10.1128/JVI.00076-06)

Work, L.M. et al. (2006) Vascular bed-targeted in vivo gene delivery using tropism-modified adeno-associated viruses. Molecular Therapy, 13(4), pp. 683-693. (doi: 10.1016/j.ymthe.2005.11.013)

2005

Baker, A., Kritz, A., Work, L., Nicklin, S. and Nicklin, A. (2005) Cell-selective viral gene delivery vectors for the vasculature. Experimental Physiology, 90(1), pp. 27-31. (doi: 10.1113/expphysiol.2004.028126)

2004

Work, L.M. , Reynolds, P. N. and Baker, A. H. (2004) Improved gene delivery to human saphenous vein cells and tissue using a peptide-modified adenoviral vector. Genetic Vaccines and Therapy, 2(1), p. 14. (doi: 10.1186/1479-0556-2-14)

Denby, L., Work, L.M., Graham, D. , Hsu, C., Von Seggern, D.J., Nicklin, S.A. and Baker, A.H. (2004) Adenoviral serotype 5 vectors pseudotyped with fibers from subgroup D show modified tropism in vitro and in vivo. Human Gene Therapy, 15(11), pp. 1054-1064.

Work, L.M., Nicol, C.G., Denby, L. and Baker, A.H. (2004) In vivo biopanning: a methodological approach to identifying novel targeting ligands for delivery of biological agents to the vasculature. In: Fennell, J.P. and Baker, A.H. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular medicine (108). Humana Press: Totowa, NJ, USA, pp. 395-413. ISBN 9781588293237 (doi: 10.1385/1-59259-850-1:395)

Work, L., Nicklin, S., Brain, N., Dishart, K., Von Seggern, D., Hallek, M., Buning, H. and Baker, A. (2004) Development of efficient viral vectors selective for vascular smooth muscle cells. Molecular Therapy, 9(2), pp. 198-208. (doi: 10.1016/j.ymthe.2003.11.006)

Work, L., Ritchie, N., Nicklin, S., Reynolds, P. and Baker, A. (2004) Dual targeting of gene delivery by genetic modification of adenovirus serotype 5 fibers and cell-selective transcriptional control. Gene Therapy, 11, pp. 1296-1300. (doi: 10.1038/sj.gt.3302292)

2003

Dishart, K.L., Work, L.M. , Denby, L. and Baker, A.H. (2003) Gene therapy for cardiovascular disease. Journal of Biomedicine and Biotechnology, 2003(2), pp. 138-148. (doi: 10.1155/S1110724303209086)

Work, L., Nicklin, S. and Baker, A. (2003) Targeting Gene Therapy Vectors to the Vascular Endothelium. Current Atherosclerosis Reports, 5(3), pp. 163-170.

2002

Wainwright, C.L., Miller, A.M., Work, L.M. and Del Soldato, P. (2002) NCX4016 (NO-aspirin) reduces infarct size and suppresses arrhythmias following myocardial ischaemia/reperfusion in pigs. British Journal of Pharmacology, 135(8), pp. 1882-1888. (doi: 10.1038/sj.bjp.0704646)

Work, L. M. , Nicklin, S. A. , White, S. J. and Baker, A. H. (2002) Use of phage display to identify novel peptides for targeted gene therapy. In: Phillips, M. I. (ed.) Gene Therapy Methods. Series: Methods in enzymology (346). Academic Press: San Diego, California ; London, pp. 157-176. ISBN 9780121822477 (doi: 10.1016/S0076-6879(02)46055-7)

2001

Work, L.M. , McPhaden, A.R., Pyne, N.J., Pyne, S., Wadsworth, R.M. and Wainwright, C.L. (2001) Short-term local delivery of an inhibitor of ras farnesyltransferase prevents neointima formation in vivo after porcine coronary balloon angioplasty. Circulation, 104(13), pp. 1538-1543. (doi: 10.1161/​hc3801.095661)

Nicklin, S., Von Seggern, D., Work, L., Pek, D., Dominiczak, A., Nemerow, G. and Baker, A. (2001) Ablating adenovirus type 5 fiber-CAR binding and HI loop insertion of the SIGYPLP peptide generate an endothelial cell-selective adenovirus. Molecular Therapy, 4(6), pp. 534-542. (doi: 10.1006/mthe.2001.0489)

1998

Kennedy, S. , Work, L. , Ferris, P., Miller, A.M., McManus, B., Wadsworth, R.M. and Wainwright, C.L. (1998) Role of nitric oxide and free radicals in the contractile response to non-preactivated leukocytes. European Journal of Pharmacology, 345(3), pp. 269-277. (doi: 10.1016/S0014-2999(98)00007-7)

This list was generated on Mon Feb 26 14:50:28 2024 GMT.
Number of items: 47.

Articles

Alghanimy, A., Work, L. M. and Holmes, W. M. (2024) The glymphatic system and multiple sclerosis: An evolving connection. Multiple Sclerosis and Related Disorders, 83, 105456. (doi: 10.1016/j.msard.2024.105456)

McFall, A. , Graham, D. , Nicklin, S. A. and Work, L. M. (2024) Unscheduled changes in pre-clinical stroke model housing contributes to variance in physiological and behavioural data outcomes- a post-hoc analysis. Brain and Neuroscience Advances, (Accepted for Publication)

Freeman-Jones, E., Miller, W. H. , Work, L. M. and Fullerton, J. L. (2023) Polypathologies and animal models of traumatic brain injury. Brain Sciences, 13(12), 1709. (doi: 10.3390/brainsci13121709) (PMID:38137157) (PMCID:PMC10741988)

Lyon, M., Fullerton, J. L. , Kennedy, S. and Work, L. M. (2023) Hypertension & dementia: pathophysiology & potential utility of antihypertensives in reducing disease burden. Pharmacology and Therapeutics, (doi: 10.1016/j.pharmthera.2023.108575) (PMID:38052309) (In Press)

Sri, S. et al. (2023) A multi-disciplinary commentary on preclinical research to investigate vascular contributions to dementia. Cerebral Circulation - Cognition and Behavior, 5, 100189. (doi: 10.1016/j.cccb.2023.100189) (PMID:37941765) (PMCID:PMC10628644)

Fullerton, J. L. , Thomas, J. M., Gonzalez-Trueba, L., Trivett, C., Van Kralingen, J. C., Allan, S. M., Quinn, T. J. and Work, L. M. (2022) Systematic review: association between circulating microRNA expression & stroke. Journal of Cerebral Blood Flow and Metabolism, 42(6), pp. 935-951. (doi: 10.1177/0271678X221085090) (PMID:35240874) (PMCID:PMC9121526)

Fullerton, J. L. , Cosgrove, C. C., Rooney, R. A. and Work, L. (2022) Extracellular vesicles and their microRNA cargo in ischaemic stroke. Journal of Physiology, (doi: 10.1113/JP282050) (PMID:35421904) (Early Online Publication)

Alshuhri, M. S. , Gallagher, L., Work, L. and Holmes, W. (2021) Direct imaging of glymphatic flow using H217O MRI. JCI Insight, 6(10), e141159. (doi: 10.1172/jci.insight.141159) (PMID:33857020)

McFall, A. , Nicklin, S. A. and Work, L. M. (2020) The counter regulatory axis of the renin angiotensin system in the brain and ischaemic stroke: insight from preclinical stroke studies and therapeutic potential. Cellular Signalling, 76, 109809. (doi: 10.1016/j.cellsig.2020.109809) (PMID:33059037) (PMCID:PMC7550360)

Pashova, A., Work, L.M. and Nicklin, S.A. (2020) The role of extracellular vesicles in neointima formation post vascular injury. Cellular Signalling, 76, 109783. (doi: 10.1016/j.cellsig.2020.109783) (PMID:32956789)

McFall, A. et al. (2020) UK consensus on pre-clinical vascular cognitive impairment functional outcomes assessment: questionnaire and workshop proceedings. Journal of Cerebral Blood Flow and Metabolism, 40(7), pp. 1402-1414. (doi: 10.1177/0271678X20910552) (PMID:32151228) (PMCID:PMC7307003)

Deuchar, G. A., Van Kralingen, J. C., Work, L. M. , Santosh, C., Muir, K. W. , McCabe, C. and Macrae, I. M. (2019) Preclinical validation of the therapeutic potential of Glasgow Oxygen Level Dependent (GOLD) technology: A theranostic for acute stroke. Translational Stroke Research, 10(5), pp. 583-595. (doi: 10.1007/s12975-018-0679-y) (PMID:30506268)

van Kralingen, J., McFall, A. , Ord, E. N.J. , Coyle, T. F., Bissett, M., McClure, J. D. , McCabe, C. , Macrae, I. M., Dawson, J. and Work, L. M. (2019) Altered extracellular vesicle microrna expression in ischemic stroke and small vessel disease. Translational Stroke Research, 10(5), pp. 495-508. (doi: 10.1007/s12975-018-0682-3) (PMID:30617992) (PMCID:PMC6733813)

Arroja, M.M.C., Reid, E. , Roy, L.A., Vallatos, A.V. , Holmes, W.M. , Nicklin, S.A. , Work, L.M. and McCabe, C. (2019) Assessing the effects of Ang-(1-7) therapy following transient middle cerebral artery occlusion. Scientific Reports, 9, 3154. (doi: 10.1038/s41598-019-39102-8) (PMID:30816157) (PMCID:PMC6395816)

Hietamies, T. M., Ostrowski, C., Pei, Z., Feng, L., McCabe, C., Work, L. M. and Quinn, T. J. (2018) Variability of functional outcome measures used in animal models of stroke and vascular cognitive impairment – a review of contemporary studies. Journal of Cerebral Blood Flow and Metabolism, 38(11), pp. 1872-1884. (doi: 10.1177/0271678X18799858) (PMID:30203705)

Horsburgh, K. et al. (2018) Small vessels, dementia and chronic diseases – molecular mechanisms and pathophysiology. Clinical Science, 132(8), pp. 851-868. (doi: 10.1042/CS20171620) (PMID:29712883)

Chouchani, E. T., Pell, V. R., James, A. M., Work, L. M. , Saeb-Parsy, K., Frezza, C., Krieg, T. and Murphy, M. P. (2016) A unifying mechanism for mitochondrial superoxide production during ischemia-reperfusion injury. Cell Metabolism, 23(2), pp. 254-263. (doi: 10.1016/j.cmet.2015.12.009) (PMID:26777689)

Akehurst, C. et al. (2015) Differential expression of microRNA-206 and its target genes in pre-eclampsia. Journal of Hypertension, 33(10), pp. 2068-2074. (doi: 10.1097/HJH.0000000000000656) (PMID:26213997) (PMCID:PMC4570688)

Chouchani, E. T. et al. (2014) Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS. Nature, 515(7527), pp. 431-435. (doi: 10.1038/nature13909) (PMID:25383517) (PMCID:PMC4255242)

Shirley, R., Ord, E. N.J. and Work, L. M. (2014) Oxidative stress and the use of antioxidants in stroke. Antioxidants, 3(3), pp. 472-501. (doi: 10.3390/antiox3030472)

Ord, E. N.J. , Shirley, R., McClure, J. D. , McCabe, C. , Kremer, E. J., Macrae, I. M. and Work, L. M. (2013) Combined antiapoptotic and antioxidant approach to acute neuroprotection for stroke in hypertensive rats. Journal of Cerebral Blood Flow and Metabolism, 33(8), pp. 1215-1224. (doi: 10.1038/jcbfm.2013.70) (PMID:23632970) (PMCID:PMC3734772)

Ord, E. N.J. , Shirley, R., van Kralingen, J. C., Graves, A., McClure, J. D. , Wilkinson, M., McCabe, C. , Macrae, I. M. and Work, L. M. (2012) Positive impact of pre-stroke surgery on survival following transient focal ischemia in hypertensive rats. Journal of Neuroscience Methods, 211(2), pp. 305-308. (doi: 10.1016/j.jneumeth.2012.09.001)

Flores, M., Work, L.M. , Douglas, K., Denby, L., Dominiczak, A.F. , Graham, D. and Nicklin, S.A. (2012) Angiotensin-(1-9) attenuates cardiac fibrosis in the stroke-prone spontaneously hypertensive rat via the angiotensin type 2 receptor. Hypertension, 59(2), pp. 300-307. (doi: 10.1161/HYPERTENSIONAHA.111.177485)

Greig, J.A., Shirley, R., Graham, D. , Denby, L., Dominiczak, A.F. , Work, L.M. and Baker, A. (2010) Vascular-targeting anti-oxidant therapy in a model of hypertension and stroke. Journal of Cardiovascular Pharmacology, 56(6), pp. 642-650. (doi: 10.1097/FJC.0b013e3181f8f19f)

Nicol, C. G., Denby, L., Lopez-Franco, O., Masson, R., Halliday, C. A., Nicklin, S. A., Kritz, A., Work, L. M. and Baker, A. H. (2009) Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature. FEBS Letters, 583(12), pp. 2100-2107. (doi: 10.1016/j.febslet.2009.05.037)

Graham, T., McIntosh, J., Work, L. , Nathwani, A. and Baker, A. (2008) Performance of AAV8 vectors expressing human factor IX from a hepatic-selective promoter following intravenous injection into rats. Genetic Vaccines and Therapy, 6(1), p. 9. (doi: 10.1186/1479-0556-6-9)

White, K. et al. (2008) Engineering adeno-associated virus 2 vectors for targeted gene delivery to atherosclerotic lesions. Gene Therapy, 15(6), pp. 443-451. (doi: 10.1038/sj.gt.3303077)

Kritz, A. B., Nicol, C. G., Dishart, K. L., Nelson, R., Holbeck, S., Von Seggern, D. J., Work, L. M., Mcvey, J. H., Nicklin, S. A. and Baker, A. H. (2007) Adenovirus 5 fibers mutated at the putative HSPG-binding site show restricted retargeting with targeting peptides in the HI loop. Molecular Therapy, 15, pp. 741-749. (doi: 10.1038/sj.mt.6300094)

Baker, A.H. et al. (2007) Brain protection using autologous bone marrow cell, metalloproteinase inhibitors, and metabolic treatment in cerebral ischemia. Proceedings of the National Academy of Sciences of the United States of America, 104, pp. 3597-3602. (doi: 10.1073/pnas.0611112104)

Denby, L., Work, L.M., Von Seggern, D.J., Wu, E., Mcvey, J.H., Nicklin, S.A. and Baker, A.H. (2007) Development of renal-targeted vectors through combined in vivo phage display and capsid engineering of adenoviral fibers from serotype 19p. Molecular Therapy, 15(9), pp. 1647-1654. (doi: 10.1038/sj.mt.6300214)

Perabo, L. et al. (2006) Heparan sulfate proteoglycan binding properties of adeno-associated virus retargeting mutants and consequences for their in vivo tropism. Journal of Virology, 80(14), pp. 7265-7269. (doi: 10.1128/JVI.00076-06)

Work, L.M. et al. (2006) Vascular bed-targeted in vivo gene delivery using tropism-modified adeno-associated viruses. Molecular Therapy, 13(4), pp. 683-693. (doi: 10.1016/j.ymthe.2005.11.013)

Baker, A., Kritz, A., Work, L., Nicklin, S. and Nicklin, A. (2005) Cell-selective viral gene delivery vectors for the vasculature. Experimental Physiology, 90(1), pp. 27-31. (doi: 10.1113/expphysiol.2004.028126)

Work, L.M. , Reynolds, P. N. and Baker, A. H. (2004) Improved gene delivery to human saphenous vein cells and tissue using a peptide-modified adenoviral vector. Genetic Vaccines and Therapy, 2(1), p. 14. (doi: 10.1186/1479-0556-2-14)

Denby, L., Work, L.M., Graham, D. , Hsu, C., Von Seggern, D.J., Nicklin, S.A. and Baker, A.H. (2004) Adenoviral serotype 5 vectors pseudotyped with fibers from subgroup D show modified tropism in vitro and in vivo. Human Gene Therapy, 15(11), pp. 1054-1064.

Work, L., Nicklin, S., Brain, N., Dishart, K., Von Seggern, D., Hallek, M., Buning, H. and Baker, A. (2004) Development of efficient viral vectors selective for vascular smooth muscle cells. Molecular Therapy, 9(2), pp. 198-208. (doi: 10.1016/j.ymthe.2003.11.006)

Work, L., Ritchie, N., Nicklin, S., Reynolds, P. and Baker, A. (2004) Dual targeting of gene delivery by genetic modification of adenovirus serotype 5 fibers and cell-selective transcriptional control. Gene Therapy, 11, pp. 1296-1300. (doi: 10.1038/sj.gt.3302292)

Dishart, K.L., Work, L.M. , Denby, L. and Baker, A.H. (2003) Gene therapy for cardiovascular disease. Journal of Biomedicine and Biotechnology, 2003(2), pp. 138-148. (doi: 10.1155/S1110724303209086)

Work, L., Nicklin, S. and Baker, A. (2003) Targeting Gene Therapy Vectors to the Vascular Endothelium. Current Atherosclerosis Reports, 5(3), pp. 163-170.

Wainwright, C.L., Miller, A.M., Work, L.M. and Del Soldato, P. (2002) NCX4016 (NO-aspirin) reduces infarct size and suppresses arrhythmias following myocardial ischaemia/reperfusion in pigs. British Journal of Pharmacology, 135(8), pp. 1882-1888. (doi: 10.1038/sj.bjp.0704646)

Work, L.M. , McPhaden, A.R., Pyne, N.J., Pyne, S., Wadsworth, R.M. and Wainwright, C.L. (2001) Short-term local delivery of an inhibitor of ras farnesyltransferase prevents neointima formation in vivo after porcine coronary balloon angioplasty. Circulation, 104(13), pp. 1538-1543. (doi: 10.1161/​hc3801.095661)

Nicklin, S., Von Seggern, D., Work, L., Pek, D., Dominiczak, A., Nemerow, G. and Baker, A. (2001) Ablating adenovirus type 5 fiber-CAR binding and HI loop insertion of the SIGYPLP peptide generate an endothelial cell-selective adenovirus. Molecular Therapy, 4(6), pp. 534-542. (doi: 10.1006/mthe.2001.0489)

Kennedy, S. , Work, L. , Ferris, P., Miller, A.M., McManus, B., Wadsworth, R.M. and Wainwright, C.L. (1998) Role of nitric oxide and free radicals in the contractile response to non-preactivated leukocytes. European Journal of Pharmacology, 345(3), pp. 269-277. (doi: 10.1016/S0014-2999(98)00007-7)

Book Sections

McFall, A. , Dawson, J. and Work, L. M. (2019) Stroke. In: Touyz, R. M. and Delles, C. (eds.) Textbook of Vascular Medicine. Springer: Cham, pp. 461-472. ISBN 9783030164805 (doi: 10.1007/978-3-030-16481-2_43)

Work, L.M. , Dominiczak, A.F. and McBride, M.W. (2009) The genetic basis of stroke: animal models. In: 3rd Report on Stroke: Genetic research in prevention and in cure. Il Pensiero Scientifico Editore, pp. 219-40.

Work, L.M., Nicol, C.G., Denby, L. and Baker, A.H. (2004) In vivo biopanning: a methodological approach to identifying novel targeting ligands for delivery of biological agents to the vasculature. In: Fennell, J.P. and Baker, A.H. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular medicine (108). Humana Press: Totowa, NJ, USA, pp. 395-413. ISBN 9781588293237 (doi: 10.1385/1-59259-850-1:395)

Work, L. M. , Nicklin, S. A. , White, S. J. and Baker, A. H. (2002) Use of phage display to identify novel peptides for targeted gene therapy. In: Phillips, M. I. (ed.) Gene Therapy Methods. Series: Methods in enzymology (346). Academic Press: San Diego, California ; London, pp. 157-176. ISBN 9780121822477 (doi: 10.1016/S0076-6879(02)46055-7)

This list was generated on Mon Feb 26 14:50:28 2024 GMT.

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Combined targeting of oedema and neuronal plasticity as a therapeutic for ischaemic stroke
    Office of the Chief Scientific Adviser
    2023 - 2025
     
  • Cross disciplinary capacity building in ultra-high frequency preclinical cardiovascular ultrasound
    British Heart Foundation
    2022 - 2025
     
  • Cardiac-targeted exosome-mediated delivery of angiotensin-(1-7) to treat cardiac disease
    British Heart Foundation
    2021 - 2024
     
  • Extracellular vesicle mediated microRNA delivery as a therapeutic for ischaemic stroke
    Chief Scientist Office
    2018 - 2021
     
  • Improving consistency and application of outcome measures in preclinical models of vascular cognitive impairment
    Alzheimer`s Research UK
    2018 - 2018
     
  • Pharmacological investigations on neuroinflammatory and excitotoxic mechanisms in animal model of stroke
    British Council
    2018 - 2018
     
  • Refinement in pre-clinical stroke research: investigation of morbidity and mortality and development of improved post-stroke care in rodents
    American College of Laboratory
    2015 - 2016
     
  • Angiotensin-(1-7): a novel treatment for acute and long term recovery from stroke?
    The Henry Smith Charity
    2014 - 2016
     
  • Modulation of the kynurenic pathway following stroke (ISSF STS)
    Wellcome Trust
    2013 - 2014
     
  • Modulation of miRNA as a therapeutic strategy for stroke
    Medical Research Council
    2012 - 2016
     
  • Supporting the ischaemic brain with oxygen-carrying perfluorocarbons.
    Chief Scientist Office
    2012 - 2015
     
  • A combined approach targeting oxidative stress and apoptosis in stroke
    British Heart Foundation
    2008 - 2012
     
  • A combined approach to target reactive oxygen species and neuronal death in stroke
    British Heart Foundation
    2007 - 2010
     

Supervision

  • Blaikie, Zachariel
    Determining the role of NADPH oxidase 5 in stroke, cerebral small vessel disease, and vascular dementia

Teaching

Lorraine successfully completed her PG Cert in Academic Practice becoming a Fellow of the Higher Education Academy in 2009. Currently, she lectures for the MSc Cardiovascular Sciences, MRes/MSc in Biomedical Sciences and to levels 3 and 4 for the Pharmacology, Neuroscience and Human Biology degrees. Dr Work leads the Clinical Neuroscience specialisation of the BMed Sci intercalated degree for medical students. Lorraine is an advisor of studies for undergraduate and postgraduate students. She has previously facilitated PBL sessions for undergraduate medical students (years 1 and/or year 2) as well as being a tutor for L3 Pharmacology for many years.

A significant portion of Lorraine’s teaching time is spent on project supervision and assessment for wet laboratory-based projects for L4 students (Pharmacology, Neuroscience), intercalating BMed Sci students and MRes/MSc students (IMB/Systems Biology and Clinical Pharmacology). Further in assessment of the research project write up/students laboratory performance, the oral "viva" style exam and assessing the oral presentations.

Dr Work is co-director of the flagship BHF 4 year PhD program (since 2021). In the MRes year, this includes feedback meetings to discuss project selection and progress; assessment of journal club presentations, review articles and the grant proposal; assessment of project report write up, oral presentations (start and end of each rotation project).

Professional activities & recognition

Prizes, awards & distinctions

  • 2021: Research Culture Award (University of Glasgow)
  • 2022: Highly Commended MVLS Engaged Researcher of the Year (University of Glasgow)
  • 2022: Incredible Impact Award (ICCA/BestCities)

Research fellowships

  • 2007 - 2008: Intermediate Fellowship, British Heart Foundation

Editorial boards

  • 2018 - Present: Cardiovascular Research

Professional & learned societies

  • 2020 - Present: Board Member, UK Society of Extracellular Vesicles
  • 2019 - 2023: Board Member, ARUK Scotland Network
  • 2019 - 2022: Co-chair Local Organising Committee, BRAIN & BRAIN PET 2022, International Society for Cerebral Blood Flow & Metabolism

Supplementary

  • SCMH/MVLS Responsibilities: Athena Swan Champion; MVLS EDI committee member