Dr Caroline Muellenbroich

  • Marie Curie Fellow (Physics & Astronomy)

Biography

In September 2019, I started my Marie Curie Fellowship "Optoheart" which develops novel optical technology in collaboration with Prof. Godfrey Smith from the Institute of Cardiovascular & Medical Sciences at Glasgow University. In 2021 I will start my permanent lectureship position at the School of Physics and Astronomy.

I studied physics at the University of Heidelberg, Germany and obtained my PhD from the Institute of Photonics, University of Strathclyde, Glasgow in 2012 with a thesis on the application of adaptive optics in advanced microscopy techniques. I then joined the Biophotonics group at the European Laboratory for Nonlinear Spectroscopy (LENS) in Florence, Italy as a post doc in 2013 to implement confocal light-sheet microscopy for structural whole mouse brain imaging and then later functional calcium imaging in Zebrafish. From 2016 on, I was a researcher with the Italian National Institute of Optics, part of the Italian National Research Council. I joined the University of Glasgow in 2018 as a fixed-term lecturer.


Research interests

Current Research

My main research interest is the development of novel imaging platforms, more specifically, practical and affordable microscopes capable of answering relevant biological questions for boosting transdisciplinary research at the interface between optical engineering and the life sciences.

You can find my publications on google scholar

Past Research

I devoted a large part of my research activities to the development of cutting-edge light-sheet microscopes for the neurosciences. After a first bespoke system for the acquisition of entire clarified mouse brains, I developed a light-sheet microscope dedicated to the functional imaging of Zebrafish larva at cellular resolution. Exploiting these results, so far limited to single plane acquisitions, I then led the development of a more sophisticated system that will eventually allow fast volumetric imaging of the entire encephalon while exposing the larva to a virtual reality. Other technological developments included a novel multi-spot microscope for semi-cleared tissues, the invention of an new image-based autofocusing system (patent pending) and the application of patterned optogenetic stimulation by means of a DMD coupled to a high-powered LED light engine.

Contact

Dr. Caroline Muellenbroich
Tel: +44 (0) 141 330 2395
email: caroline.muellenbroich@glasgow.ac.uk
twitter: @cmuell15

Office 233a
Kelvin Building, G12 8QQ
School of Physics and Astronomy
University of Glasgow

researcherID: G-8217-2017


Publications

List by: Type | Date

Jump to: 2020 | 2019 | 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2011 | 2008
Number of items: 28.

2020

de Vito, G. et al. (2020) Two-photon high-speed light-sheet volumetric imaging of brain activity during sleep in zebrafish larvae. In: SPIE BiOS, 2020, San Francisco, California, United States, 1-6 Feb 2020, p. 1122604. ISBN 9781510632158 (doi:10.1117/12.2542285)

2019

Di Giovanna, A. P., Credi, C., Franceschini, A., Müllenbroich, M. C. , Silvestri, L. and Pavone, F. (2019) Tailored sample mounting for light-sheet fluorescence microscopy of clarified specimens by polydimethylsiloxane casting. Frontiers in Neuroanatomy, 13, 35. (doi: 10.3389/fnana.2019.00035)

2018

Müllenbroich, M. C. , Silvestri, L., Di Giovanna, A. P., Mazzamuto, G., Costantini, I., Sacconi, L. and Pavone, F. S. (2018) High-fidelity imaging in brain-wide structural studies using light-sheet microscopy. eNeuro, 5(6), e0124-18.2018. (doi: 10.1523/ENEURO.0124-18.2018)

Muellenbroich, M. C. , Turrini, L., Silvestri, L., Alterini, T., Gheisari, A., Vanzi, F., Sacconi, L. and Pavone, F. S. (2018) Bessel beam illumination reduces random and systematic errors in quantitative functional studies using light-sheet microscopy. Frontiers in Cellular Neuroscience, 12, 315. (doi: 10.3389/fncel.2018.00315) (PMID:30294262) (PMCID:PMC6158350)

Scardigli, M. et al. (2018) Real-time optical manipulation of cardiac conduction in intact hearts. Journal of Physiology, 596(17), pp. 3841-3858. (doi: 10.1113/jp276283) (PMID:29989169)

Di Giovanna, A. P., Tibo, A., Silvestri, L., Müllenbroich, M. C. , Costantini, I., Allegra Mascaro, A. L., Sacconi, L., Frasconi, P. and Pavone, F. S. (2018) Whole-brain vasculature reconstruction at the single capillary level. Scientific Reports, 8, 12573. (doi: 10.1038/s41598-018-30533-3) (PMID:30135559) (PMCID:PMC6105658)

Pavone, F. S., Sacconi, L., Silvestri, L., Alfieri, D., Di Giovanna, A. P., Müllenbroich, M. C. and Adam, M.-P. (2018) Confocal multispot microscope for fast and deep imaging in semicleared tissues. Journal of Biomedical Optics, 23(2), 020503. (doi: 10.1117/1.JBO.23.2.020503) (PMID:29460510)

2017

Turrini, L. et al. (2017) Optical mapping of neuronal activity during seizures in zebrafish. Scientific Reports, 7, 3025. (doi: 10.1038/s41598-017-03087-z) (PMID:28596596) (PMCID:PMC5465210)

Luo, Q. et al. (2017) High-fidelity Functional and Structural Whole-brain Imaging with Bessel-beam Light-sheet Microscopy (Conference presentation). SPIE BiOS 2017, San Francisco, CA., April 2017. 100510F. (doi:10.1117/12.2251471)

Beaurepaire, E. et al. (2017) Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy. European Conference on Biomedical Optics 2017, Munich, Germany, 25–29 June 2017. 104140N. ISBN 9781510612860 (doi:10.1117/12.2285044)

Müllenbroich, M. C. , Silvestri, L., Turrini, L., Di Giovanna, A. P., Alterini, T., Gheisari, A., Sacconi, L., vanzi, f. and Pavone, F. S. (2017) High-Fidelity Imaging with Bessel-Beam Light-Sheet Microscopy for Whole-Brain Structural and Functional Studies. Optics and the Brain 2017, San Diego, CA., 2-5 April 2017. BrW4B.2. ISBN 9781943580255 (doi:10.1364/BRAIN.2017.BrW4B.2)

Silvestri, L., Müllenbroich, M. C. , Costantini, I., Di Giovanna, A. P., Sacconi, L. and Pavone, F. S. (2017) Fast, Image-based Autofocus System for High-resolution Optical Microscopy of Whole Mouse Brains. Bio-Optics Design and Application 2017, San Diego, CA., 2-5 April 2017. JTu4A.8. ISBN 9781943580255 (doi:10.1364/BODA.2017.JTu4A.8)

2016

Turrini, L., Alterini, T., Müllenbroich, C. , Gheisari, A., Sacconi, L., Silvestri, L., Vanzi, F. and Pavone, F.S. (2016) Functional Imaging of Zebrafish Neuronal Activity by Bessel Beam Light-Sheet Microscopy. In: 18th Italian National Conference on Photonic Technologies (Fotonica 2016), Rome, Italy, 6-8 June 2016, 66 (3 .)-66 (3 .). ISBN 9781785612688 (doi:10.1049/cp.2016.0926)

Silvestri, L., Rudinskiy, N., Paciscopi, M., Müllenbroich, M. C. , Costantini, I., Sacconi, L., Frasconi, P., Hyman, B. T. and Pavone, F. S. (2016) Mapping Whole-Brain Activity with Cellular Resolution by Light-Sheet Microscopy and High-Throughput Image Analysis (Conference Presentation). Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation 2016, San Francisco, CA. p. 969012. (doi:10.1117/12.2208603)

Di Giovanna, A. P., Silvestri, L., Müllenbroich, C. , Mascaro, A. L. A., Sacconi, L. and Pavone, F. S. (2016) High-Resolution Reconstruction of Whole Mouse Brain Vasculature with Light-Sheet Microscopy. In: Optics and the Brain. Biomedical Optics 2016, Fort Lauderdale, FL., 25-28 Apr 2016, BTu4D.4. ISBN 9781943580101 (doi:10.1364/brain.2016.btu4d.4)

2015

Müllenbroich, M. C. , Silvestri, L., Onofri, L., Costantini, I., van’t Hoff, M., Sacconi, L., Iannello, G. and Pavone, F. S. (2015) Comprehensive optical and data management infrastructure for high-throughput light-sheet microscopy of whole mouse brains. Neurophotonics, 2(4), 041404. (doi: 10.1117/1.NPh.2.4.041404) (PMID:26158018) (PMCID:PMC4484248)

Costantini, I., Giovanna, A. P. D., Mascaro, A. L. A., Silvestri, L., Müllenbroich, M. C. , Sacconi, L. and Pavone, F. S. (2015) A Versatile New Technique to Clear Mouse and Human Brain. In: European Conference on Biomedical Optics 2015, Munich, Germany, 21–25 June 2015, 95360X. ISBN 9781628417012 (doi:10.1364/ecbo.2015.95360x)

Costantini, I. et al. (2015) A versatile clearing agent for multi-modal brain imaging. Scientific Reports, 5, 9808. (doi: 10.1038/srep09808) (PMID:25950610) (PMCID:PMC4423470)

Costantini, I., Allegra Mascaro, A. L., Di Giovanna, A. P., Silvestri, L., Müllenbroich, M. C. , Sacconi, L. and Pavone, F. S. (2015) A Multi Modal Clearing Method for Brain Imaging. Optics and the Brain 2015, Vancouver, Canada, 12-15 Apr 2015. BrM3B.2. ISBN 9781557529541 (doi:10.1364/boda.2015.brm3b.2)

Silvestri, L., Rudinskiy, N., Paciscopi, M., Müllenbroich, M. C. , Costantini, I., Sacconi, L., Frasconi, P., hyman, B. T. and Pavone, F. S. (2015) Brain-Wide Charting of Neuronal Activation Maps with Cellular Resolution. Optics and the Brain 2015, Vancouver, Canada, 12-15 Apr 2015. BrM3B.6. ISBN 9781557529541 (doi:10.1364/BRAIN.2015.BrM3B.6)

Costantini, I., Di Giovanna, A. P., Allegra Mascaro, A. L., Silvestri, L., Müllenbroich, M. C. , Sacconi, L. and Pavone, F. S. (2015) A new versatile clearing method for brain imaging. In: Hirschberg, H., Madsen, S.J., Jansen, E.D., Luo, Q., Mohanty, S.K. and Thakor, N.V. (eds.) Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II. Series: Proceedings of SPIE (9305). SPIE, p. 930514. ISBN 9781628413953 (doi:10.1117/12.2076908)

Costantini, I., Di Giovanna, A. P., Allegra Mascaro, A. L., Silvestri, L., Müllenbroich, M. C. , Sacconi, L. and Pavone, F. S. (2015) A versatile new technique to clear mouse and human brain. In: Beaurepaire, E., So, P.T.C., Pavone, F. and Hillman, E.M. (eds.) Advanced Microscopy Techniques IV; and Neurophotonics II. Series: Proceedings of SPIE (9536). SPIE. ISBN 9781628417012 (doi:10.1117/12.2184289)

2014

Müllenbroich, M. C. , McGhee, E. J., Wright, A. J., Anderson, K. I. and Mathieson, K. (2014) Strategies to overcome photobleaching in algorithm-based adaptive optics for nonlinearin-vivoimaging. Journal of Biomedical Optics, 19(1), 016021. (doi: 10.1117/1.JBO.19.1.016021)

2013

Gramatikov, B., Irsch, K., Müllenbroich, M. , Frindt, N., Qu, Y., Gutmark, R., Wu, Y.-K. and Guyton, D. (2013) A device for continuous monitoring of true central fixation based on foveal birefringence. Annals of Biomedical Engineering, 41(9), pp. 1968-1978. (doi: 10.1007/s10439-013-0818-2) (PMID:23645511)

Müllenbroich, M.C. , McAlinden, N. and Wright, A.J. (2013) Adaptive optics in an optical trapping system for enhanced lateral trap stiffness at depth. Journal of Optics, 15(7), 075305. (doi: 10.1088/2040-8978/15/7/075305)

Müllenbroich, M. C. , McGhee, E. J., Wright, A. J., Anderson, K. I. and Mathieson, K. (2013) Adaptive nonlinear microscopy for whole tissue imaging. In: Ammasi, P., Konig, K. and So, P.T.C. (eds.) Multiphoton Microscopy in the Biomedical Sciences XIII. Series: SPIE proceedings (8588). SPIE. ISBN 9780819493576 (doi:10.1117/12.2006375)

2011

Muellenbroich, C. , McAlinden, N. and Wright, A. J. (2011) Aberration Correction in an Optical Trapping System Using a Deformable Membrane Mirror. In: Frontiers in Optics 2011/Laser Science XXVII, San Jose, California, 16-20 Oct 2011, FTuS5. ISBN 9781557529176 (doi:10.1364/FIO.2011.FTuS5)

2008

Ramey, N. A., Ying, H. S., Irsch, K., Müllenbroich, M. C. , Vaswani, R. and Guyton, D. L. (2008) A novel haploscopic viewing apparatus with a three-axis eye tracker. Journal of American Association for Pediatric Ophthalmology and Strabismus, 12(5), pp. 498-503. (doi: 10.1016/j.jaapos.2008.01.019) (PMID:18440260)

This list was generated on Tue Aug 11 13:55:58 2020 BST.