Mrs June Southall
- Technical Specialist (Molecular Biosciences)
telephone:
01413306448
email:
June.Southall@glasgow.ac.uk
B4-13 Joseph Black Building, Institute of MC&SB, University Avenue, Glasgow G12 8QQ
Biography
Key Skills
Core Funded
- Molecular biology: Cloning, Gene knockout, Gene expression
- Protein Science: Bacterial expression system, Mammalian expression systems, Native and recombinant protein expression, Protein purification, Protein crystallisation
- Biophysical characterisation: Circular Dichroism, Isothermal Titration Calorimetry, Size exclusion chromatography- Multi Angle Light Scattering, Microscale thermophoresis
Research interests
Studying protein structure and characterisation of biomolecules and their interactions.
Publications
2019
Taffet, E. J., Lee, B. G., Toa, Z. S. D., Pace, N., Rumbles, G., Southall, J. , Cogdell, R. J. and Scholes, G. D. (2019) Carotenoid nuclear reorganization and interplay of bright and dark excited states. Journal of Physical Chemistry B, 123(41), pp. 8628-8643. (doi: 10.1021/acs.jpcb.9b04027) (PMID:31553605)
2018
Southall, J. , Henry, S. L., Gardiner, A. T., Roszak, A. W., Mullen, W. , Carey, A.-M., Kelly, S. M. , de Percin Northumberland, C. O. and Cogdell, R. J. (2018) Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes. Photosynthesis Research, 135(1-3), pp. 9-21. (doi: 10.1007/s11120-017-0386-7) (PMID:28567613)
2017
Kuznetsova, V., Southall, J. , Cogdell, R. J. , Fuciman, M. and Polívka, T. (2017) Spectroscopic properties of the S1 state of linear carotenoids after excess energy excitation. Chemical Physics Letters, 683, pp. 448-453. (doi: 10.1016/j.cplett.2017.03.009)
2016
Ferretti, M., Hendrikx, R., Romero, E., Southall, J. , Cogdell, R. J. , Novoderezhkin, V. I., Scholes, G. D. and van Grondelle, R. (2016) Dark states in the light-harvesting complex 2 revealed by two-dimensional electronic spectroscopy. Scientific Reports, 6(1), 20834. (doi: 10.1038/srep20834) (PMID:26857477) (PMCID:PMC4746630)
Miki, T., Buckup, T., Krause, M. S., Southall, J. , Cogdell, R. J. and Motzkus, M. (2016) Vibronic coupling in the excited-states of carotenoids. Physical Chemistry Chemical Physics, 18(16), pp. 11443-11453. (doi: 10.1039/C5CP07542D) (PMID:27055720)
2015
Beyer, S. R., Müller, L., Southall, J. , Cogdell, R. J. , Ullmann, G. M. and Köhler, J. (2015) The open, the closed, and the empty: time-resolved fluorescence spectroscopy and computational analysis of RC-LH1 complexes from Rhodopseudomonas palustris. Journal of Physical Chemistry B, 119(4), pp. 1362-1373. (doi: 10.1021/jp510822k) (PMID:25526393)
2013
Kunz, R., Timpmann, K., Southall, J. , Cogdell, R.J. , Freiberg, A. and Köhler, J. (2013) Fluctuations in the electron-phonon coupling of a single chromoprotein. Angewandte Chemie (International Edition), 52(33), pp. 8726-8730. (doi: 10.1002/anie.201303231)
Böhm, P.S., Southall, J. , Cogdell, R.J. and Köhler, J. (2013) Single-molecule spectroscopy on RC-LH1 complexes of rhodopseudomonas acidophilaStrain 10050. Journal of Physical Chemistry B, 117(11), pp. 3120-3126. (doi: 10.1021/jp4005218)
Böhm, P.S., Kunz, R., Southall, J. , Cogdell, R.J. and Köhler, J. (2013) Does the reconstitution of RC-LH1 complexes from Rhodopseudomonas acidophilaStrain 10050 into a Phospholipid Bilayer yield the optimum environment for Optical spectroscopy? Journal of Physical Chemistry B, 117(48), pp. 15004-15013. (doi: 10.1021/jp409980k)
Kunz, R., Timpmann, K., Southall, J. , Cogdell, R.J. , Köhler, J., and Freiberg, A. (2013) Fluorescence-excitation and emission spectra from LH2 antenna complexes of rhodopseudomonas acidophila as a function of the sample preparation conditions. Journal of Physical Chemistry B, 117(40), pp. 12020-12029. (doi: 10.1021/jp4073697)
Marek, M.S., Buckup, T., Southall, J. , Cogdell, R.J. and Motzkus, M. (2013) Highlighting short-lived excited electronic states with pump-degenerate-four-wave-mixing. Journal of Chemical Physics, 139(7), 074202. (doi: 10.1063/1.4818164)
2012
Kunz, R., Timpmann, K., Southall, J. , Cogdell, R.J. , Freiberg, A. and Köhler, J. (2012) Exciton self trapping in photosynthetic pigment–protein complexes studied by single-molecule spectroscopy. Journal of Physical Chemistry B, 116(36), pp. 11017-11023. (doi: 10.1021/jp3040456)
2011
Pflock, T.J., Oellerich, S., Southall, J. , Cogdell, R.J. , Ullmann, G.M. and Köhler, J. (2011) The electronically excited states of LH2 complexes from Rhodopseudomonas acidophila strain 10050 studied by time-resolved spectroscopy and dynamic Monte Carlo simulations. I. Isolated, non-interacting LH2 complexes. Journal of Physical Chemistry B, 115(28), pp. 8813-8820. (doi: 10.1021/jp202353c)
Pflock, T.J., Oellerich, S., Krapf, L., Southall, J. , Cogdell, R.J. , Ullmann, G.m. and Köhler, J. (2011) The Electronically Excited States of LH2 Complexes from Rhodopseudomonas acidophila Strain 10050 Studied by Time-Resolved Spectroscopy and Dynamic Monte Carlo Simulations. II. Homo-Arrays Of LH2 Complexes Reconstituted Into Phospholipid Model Membranes. Journal of Physical Chemistry B, 115(28), pp. 8821-8831. (doi: 10.1021/jp2023583)
Unterkofler, S., Pflock, T., Southall, J. , Cogdell, R.J. and Köhler, J. (2011) Fluorescence blinking of the RC-LH1 complex from Rhodopseudomonas palustris. ChemPhysChem, 12(3), pp. 711-716. (doi: 10.1002/cphc.201000588)
Articles
Taffet, E. J., Lee, B. G., Toa, Z. S. D., Pace, N., Rumbles, G., Southall, J. , Cogdell, R. J. and Scholes, G. D. (2019) Carotenoid nuclear reorganization and interplay of bright and dark excited states. Journal of Physical Chemistry B, 123(41), pp. 8628-8643. (doi: 10.1021/acs.jpcb.9b04027) (PMID:31553605)
Southall, J. , Henry, S. L., Gardiner, A. T., Roszak, A. W., Mullen, W. , Carey, A.-M., Kelly, S. M. , de Percin Northumberland, C. O. and Cogdell, R. J. (2018) Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes. Photosynthesis Research, 135(1-3), pp. 9-21. (doi: 10.1007/s11120-017-0386-7) (PMID:28567613)
Kuznetsova, V., Southall, J. , Cogdell, R. J. , Fuciman, M. and Polívka, T. (2017) Spectroscopic properties of the S1 state of linear carotenoids after excess energy excitation. Chemical Physics Letters, 683, pp. 448-453. (doi: 10.1016/j.cplett.2017.03.009)
Ferretti, M., Hendrikx, R., Romero, E., Southall, J. , Cogdell, R. J. , Novoderezhkin, V. I., Scholes, G. D. and van Grondelle, R. (2016) Dark states in the light-harvesting complex 2 revealed by two-dimensional electronic spectroscopy. Scientific Reports, 6(1), 20834. (doi: 10.1038/srep20834) (PMID:26857477) (PMCID:PMC4746630)
Miki, T., Buckup, T., Krause, M. S., Southall, J. , Cogdell, R. J. and Motzkus, M. (2016) Vibronic coupling in the excited-states of carotenoids. Physical Chemistry Chemical Physics, 18(16), pp. 11443-11453. (doi: 10.1039/C5CP07542D) (PMID:27055720)
Beyer, S. R., Müller, L., Southall, J. , Cogdell, R. J. , Ullmann, G. M. and Köhler, J. (2015) The open, the closed, and the empty: time-resolved fluorescence spectroscopy and computational analysis of RC-LH1 complexes from Rhodopseudomonas palustris. Journal of Physical Chemistry B, 119(4), pp. 1362-1373. (doi: 10.1021/jp510822k) (PMID:25526393)
Kunz, R., Timpmann, K., Southall, J. , Cogdell, R.J. , Freiberg, A. and Köhler, J. (2013) Fluctuations in the electron-phonon coupling of a single chromoprotein. Angewandte Chemie (International Edition), 52(33), pp. 8726-8730. (doi: 10.1002/anie.201303231)
Böhm, P.S., Southall, J. , Cogdell, R.J. and Köhler, J. (2013) Single-molecule spectroscopy on RC-LH1 complexes of rhodopseudomonas acidophilaStrain 10050. Journal of Physical Chemistry B, 117(11), pp. 3120-3126. (doi: 10.1021/jp4005218)
Böhm, P.S., Kunz, R., Southall, J. , Cogdell, R.J. and Köhler, J. (2013) Does the reconstitution of RC-LH1 complexes from Rhodopseudomonas acidophilaStrain 10050 into a Phospholipid Bilayer yield the optimum environment for Optical spectroscopy? Journal of Physical Chemistry B, 117(48), pp. 15004-15013. (doi: 10.1021/jp409980k)
Kunz, R., Timpmann, K., Southall, J. , Cogdell, R.J. , Köhler, J., and Freiberg, A. (2013) Fluorescence-excitation and emission spectra from LH2 antenna complexes of rhodopseudomonas acidophila as a function of the sample preparation conditions. Journal of Physical Chemistry B, 117(40), pp. 12020-12029. (doi: 10.1021/jp4073697)
Marek, M.S., Buckup, T., Southall, J. , Cogdell, R.J. and Motzkus, M. (2013) Highlighting short-lived excited electronic states with pump-degenerate-four-wave-mixing. Journal of Chemical Physics, 139(7), 074202. (doi: 10.1063/1.4818164)
Kunz, R., Timpmann, K., Southall, J. , Cogdell, R.J. , Freiberg, A. and Köhler, J. (2012) Exciton self trapping in photosynthetic pigment–protein complexes studied by single-molecule spectroscopy. Journal of Physical Chemistry B, 116(36), pp. 11017-11023. (doi: 10.1021/jp3040456)
Pflock, T.J., Oellerich, S., Southall, J. , Cogdell, R.J. , Ullmann, G.M. and Köhler, J. (2011) The electronically excited states of LH2 complexes from Rhodopseudomonas acidophila strain 10050 studied by time-resolved spectroscopy and dynamic Monte Carlo simulations. I. Isolated, non-interacting LH2 complexes. Journal of Physical Chemistry B, 115(28), pp. 8813-8820. (doi: 10.1021/jp202353c)
Pflock, T.J., Oellerich, S., Krapf, L., Southall, J. , Cogdell, R.J. , Ullmann, G.m. and Köhler, J. (2011) The Electronically Excited States of LH2 Complexes from Rhodopseudomonas acidophila Strain 10050 Studied by Time-Resolved Spectroscopy and Dynamic Monte Carlo Simulations. II. Homo-Arrays Of LH2 Complexes Reconstituted Into Phospholipid Model Membranes. Journal of Physical Chemistry B, 115(28), pp. 8821-8831. (doi: 10.1021/jp2023583)
Unterkofler, S., Pflock, T., Southall, J. , Cogdell, R.J. and Köhler, J. (2011) Fluorescence blinking of the RC-LH1 complex from Rhodopseudomonas palustris. ChemPhysChem, 12(3), pp. 711-716. (doi: 10.1002/cphc.201000588)