Mrs June Southall
- Technical Specialist (Molecular Biosciences)
email:
June.Southall@glasgow.ac.uk
B4-13 Joseph Black Building, Institute of MC&SB, University Avenue, Glasgow G12 8QQ
Biography
Key Skills
Core Funded
- Molecular biology: Cloning, Gene knockout, Gene expression
- Protein Science: Bacterial expression system, Mammalian expression systems, Native and recombinant protein expression, Protein purification, Protein crystallisation
- Biophysical characterisation: Circular Dichroism, Isothermal Titration Calorimetry, Size exclusion chromatography- Multi Angle Light Scattering, Microscale thermophoresis
Research interests
Studying protein structure and characterisation of biomolecules and their interactions.
Publications
2022
Qian, P. et al. (2022) Cryo-EM structures of light-harvesting 2 complexes from Rhodopseudomonas palustris reveal the molecular origin of absorption tuning. Proceedings of the National Academy of Sciences of the United States of America, 117(43), e221010911. (doi: 10.1073/pnas.2210109119) (PMID:36251992) (PMCID:PMC9618040)
2019
Taffet, Elliot J., Lee, Benjamin G., Toa, Zi S. D., Pace, Natalie, Rumbles, Garry, Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Scholes, Gregory D.
(2019)
Carotenoid nuclear reorganization and interplay of bright and dark excited states.
Journal of Physical Chemistry B, 123(41),
pp. 8628-8643.
(doi: 10.1021/acs.jpcb.9b04027)
(PMID:31553605)
2018
Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Henry, Sarah L., Gardiner, Alastair T., Roszak, Aleksander W., Mullen, William
ORCID: https://orcid.org/0000-0002-5685-1563, Carey, Anne-Marie, Kelly, Sharon M.
ORCID: https://orcid.org/0000-0002-3516-1387, de Percin Northumberland, Claire Ortmann and Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607
(2018)
Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes.
Photosynthesis Research, 135(1-3),
pp. 9-21.
(doi: 10.1007/s11120-017-0386-7)
(PMID:28567613)
2017
Kuznetsova, Valentyna, Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607, Fuciman, Marcel and Polívka, Tomáš
(2017)
Spectroscopic properties of the S1 state of linear carotenoids after excess energy excitation.
Chemical Physics Letters, 683,
pp. 448-453.
(doi: 10.1016/j.cplett.2017.03.009)
2016
Ferretti, Marco, Hendrikx, Ruud, Romero, Elisabet, Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607, Novoderezhkin, Vladimir I., Scholes, Gregory D. and van Grondelle, Rienk
(2016)
Dark states in the light-harvesting complex 2 revealed by two-dimensional electronic spectroscopy.
Scientific Reports, 6,
20834.
(doi: 10.1038/srep20834)
(PMID:26857477)
(PMCID:PMC4746630)
Miki, Takeshi, Buckup, Tiago, Krause, Marie S., Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Motzkus, Marcus
(2016)
Vibronic coupling in the excited-states of carotenoids.
Physical Chemistry Chemical Physics, 18(16),
pp. 11443-11453.
(doi: 10.1039/C5CP07542D)
(PMID:27055720)
2015
Beyer, Sebastian R., Müller, Lars, Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607, Ullmann, G. Matthias and Köhler, Jürgen
(2015)
The open, the closed, and the empty: time-resolved fluorescence spectroscopy and computational analysis of RC-LH1 complexes from Rhodopseudomonas palustris.
Journal of Physical Chemistry B, 119(4),
pp. 1362-1373.
(doi: 10.1021/jp510822k)
(PMID:25526393)
2013
Kunz, R., Timpmann, K., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Freiberg, A. and Köhler, J.
(2013)
Fluctuations in the electron-phonon coupling of a single chromoprotein.
Angewandte Chemie (International Edition), 52(33),
pp. 8726-8730.
(doi: 10.1002/anie.201303231)
Böhm, P.S., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Köhler, J.
(2013)
Single-molecule spectroscopy on RC-LH1 complexes of rhodopseudomonas acidophilaStrain 10050.
Journal of Physical Chemistry B, 117(11),
pp. 3120-3126.
(doi: 10.1021/jp4005218)
Böhm, P.S., Kunz, R., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Köhler, J.
(2013)
Does the reconstitution of RC-LH1 complexes from Rhodopseudomonas acidophilaStrain 10050 into a Phospholipid Bilayer yield the optimum environment for Optical spectroscopy?
Journal of Physical Chemistry B, 117(48),
pp. 15004-15013.
(doi: 10.1021/jp409980k)
Kunz, R., Timpmann, K., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Köhler, J, and Freiberg, A.
(2013)
Fluorescence-excitation and emission spectra from LH2 antenna complexes of rhodopseudomonas acidophila as a function of the sample preparation conditions.
Journal of Physical Chemistry B, 117(40),
pp. 12020-12029.
(doi: 10.1021/jp4073697)
Marek, M.S., Buckup, T., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Motzkus, M.
(2013)
Highlighting short-lived excited electronic states with pump-degenerate-four-wave-mixing.
Journal of Chemical Physics, 139(7),
074202.
(doi: 10.1063/1.4818164)
2012
Kunz, R., Timpmann, K., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Freiberg, A. and Köhler, J.
(2012)
Exciton self trapping in photosynthetic pigment–protein complexes studied by single-molecule spectroscopy.
Journal of Physical Chemistry B, 116(36),
pp. 11017-11023.
(doi: 10.1021/jp3040456)
2011
Pflock, T.J., Oellerich, S., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Ullmann, G.M. and Köhler, J.
(2011)
The electronically excited states of LH2 complexes from Rhodopseudomonas acidophila strain 10050 studied by time-resolved spectroscopy and dynamic Monte Carlo simulations. I. Isolated, non-interacting LH2 complexes.
Journal of Physical Chemistry B, 115(28),
pp. 8813-8820.
(doi: 10.1021/jp202353c)
Pflock, T.J., Oellerich, S., Krapf, L., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Ullmann, G.m. and Köhler, J.
(2011)
The Electronically Excited States of LH2 Complexes from Rhodopseudomonas acidophila Strain 10050 Studied by Time-Resolved Spectroscopy and Dynamic Monte Carlo Simulations. II. Homo-Arrays Of LH2 Complexes Reconstituted Into Phospholipid Model Membranes.
Journal of Physical Chemistry B, 115(28),
pp. 8821-8831.
(doi: 10.1021/jp2023583)
Unterkofler, S., Pflock, T., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Köhler, J.
(2011)
Fluorescence blinking of the RC-LH1 complex from Rhodopseudomonas palustris.
ChemPhysChem, 12(3),
pp. 711-716.
(doi: 10.1002/cphc.201000588)
Articles
Qian, P. et al. (2022) Cryo-EM structures of light-harvesting 2 complexes from Rhodopseudomonas palustris reveal the molecular origin of absorption tuning. Proceedings of the National Academy of Sciences of the United States of America, 117(43), e221010911. (doi: 10.1073/pnas.2210109119) (PMID:36251992) (PMCID:PMC9618040)
Taffet, Elliot J., Lee, Benjamin G., Toa, Zi S. D., Pace, Natalie, Rumbles, Garry, Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Scholes, Gregory D.
(2019)
Carotenoid nuclear reorganization and interplay of bright and dark excited states.
Journal of Physical Chemistry B, 123(41),
pp. 8628-8643.
(doi: 10.1021/acs.jpcb.9b04027)
(PMID:31553605)
Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Henry, Sarah L., Gardiner, Alastair T., Roszak, Aleksander W., Mullen, William
ORCID: https://orcid.org/0000-0002-5685-1563, Carey, Anne-Marie, Kelly, Sharon M.
ORCID: https://orcid.org/0000-0002-3516-1387, de Percin Northumberland, Claire Ortmann and Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607
(2018)
Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes.
Photosynthesis Research, 135(1-3),
pp. 9-21.
(doi: 10.1007/s11120-017-0386-7)
(PMID:28567613)
Kuznetsova, Valentyna, Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607, Fuciman, Marcel and Polívka, Tomáš
(2017)
Spectroscopic properties of the S1 state of linear carotenoids after excess energy excitation.
Chemical Physics Letters, 683,
pp. 448-453.
(doi: 10.1016/j.cplett.2017.03.009)
Ferretti, Marco, Hendrikx, Ruud, Romero, Elisabet, Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607, Novoderezhkin, Vladimir I., Scholes, Gregory D. and van Grondelle, Rienk
(2016)
Dark states in the light-harvesting complex 2 revealed by two-dimensional electronic spectroscopy.
Scientific Reports, 6,
20834.
(doi: 10.1038/srep20834)
(PMID:26857477)
(PMCID:PMC4746630)
Miki, Takeshi, Buckup, Tiago, Krause, Marie S., Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Motzkus, Marcus
(2016)
Vibronic coupling in the excited-states of carotenoids.
Physical Chemistry Chemical Physics, 18(16),
pp. 11443-11453.
(doi: 10.1039/C5CP07542D)
(PMID:27055720)
Beyer, Sebastian R., Müller, Lars, Southall, June ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, Richard J.
ORCID: https://orcid.org/0000-0003-2119-6607, Ullmann, G. Matthias and Köhler, Jürgen
(2015)
The open, the closed, and the empty: time-resolved fluorescence spectroscopy and computational analysis of RC-LH1 complexes from Rhodopseudomonas palustris.
Journal of Physical Chemistry B, 119(4),
pp. 1362-1373.
(doi: 10.1021/jp510822k)
(PMID:25526393)
Kunz, R., Timpmann, K., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Freiberg, A. and Köhler, J.
(2013)
Fluctuations in the electron-phonon coupling of a single chromoprotein.
Angewandte Chemie (International Edition), 52(33),
pp. 8726-8730.
(doi: 10.1002/anie.201303231)
Böhm, P.S., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Köhler, J.
(2013)
Single-molecule spectroscopy on RC-LH1 complexes of rhodopseudomonas acidophilaStrain 10050.
Journal of Physical Chemistry B, 117(11),
pp. 3120-3126.
(doi: 10.1021/jp4005218)
Böhm, P.S., Kunz, R., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Köhler, J.
(2013)
Does the reconstitution of RC-LH1 complexes from Rhodopseudomonas acidophilaStrain 10050 into a Phospholipid Bilayer yield the optimum environment for Optical spectroscopy?
Journal of Physical Chemistry B, 117(48),
pp. 15004-15013.
(doi: 10.1021/jp409980k)
Kunz, R., Timpmann, K., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Köhler, J, and Freiberg, A.
(2013)
Fluorescence-excitation and emission spectra from LH2 antenna complexes of rhodopseudomonas acidophila as a function of the sample preparation conditions.
Journal of Physical Chemistry B, 117(40),
pp. 12020-12029.
(doi: 10.1021/jp4073697)
Marek, M.S., Buckup, T., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Motzkus, M.
(2013)
Highlighting short-lived excited electronic states with pump-degenerate-four-wave-mixing.
Journal of Chemical Physics, 139(7),
074202.
(doi: 10.1063/1.4818164)
Kunz, R., Timpmann, K., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Freiberg, A. and Köhler, J.
(2012)
Exciton self trapping in photosynthetic pigment–protein complexes studied by single-molecule spectroscopy.
Journal of Physical Chemistry B, 116(36),
pp. 11017-11023.
(doi: 10.1021/jp3040456)
Pflock, T.J., Oellerich, S., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Ullmann, G.M. and Köhler, J.
(2011)
The electronically excited states of LH2 complexes from Rhodopseudomonas acidophila strain 10050 studied by time-resolved spectroscopy and dynamic Monte Carlo simulations. I. Isolated, non-interacting LH2 complexes.
Journal of Physical Chemistry B, 115(28),
pp. 8813-8820.
(doi: 10.1021/jp202353c)
Pflock, T.J., Oellerich, S., Krapf, L., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607, Ullmann, G.m. and Köhler, J.
(2011)
The Electronically Excited States of LH2 Complexes from Rhodopseudomonas acidophila Strain 10050 Studied by Time-Resolved Spectroscopy and Dynamic Monte Carlo Simulations. II. Homo-Arrays Of LH2 Complexes Reconstituted Into Phospholipid Model Membranes.
Journal of Physical Chemistry B, 115(28),
pp. 8821-8831.
(doi: 10.1021/jp2023583)
Unterkofler, S., Pflock, T., Southall, J. ORCID: https://orcid.org/0000-0002-5496-1531, Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Köhler, J.
(2011)
Fluorescence blinking of the RC-LH1 complex from Rhodopseudomonas palustris.
ChemPhysChem, 12(3),
pp. 711-716.
(doi: 10.1002/cphc.201000588)