Dr Stephen Carter

  • Research Fellow (Virology)

Research interests

Our work focusses on better understanding the intricate structural interactions between viruses and host complexes during virus infection and the immune response during restriction. To do this we visualize these interactions in a frozen-hydrated state at macromolecular resolution using cryo-electron tomography (cryo-ET). To target specific events or rare processes we use cryogenic correlated light and electron microscopy (cryo-CLEM) in combination with cryo-ET. To gain access to the entire cell, we use this approach with cryogenic focused ion beam (cryo-FIB) milling.

Publications

List by: Type | Date

Jump to: 2022 | 2021 | 2020 | 2019 | 2018 | 2016 | 2012 | 2010
Number of items: 14.

2022

Haney, J., Vijayakrishnan, S. , Streetley, J. , Dee, K., Goldfarb, D. M., Clarke, M. , Mullin, M., Carter, S. D. , Bhella, D. and Murcia, P. R. (2022) Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles. Nature Microbiology, 7(11), pp. 1879-1890. (doi: 10.1038/s41564-022-01242-5) (PMID:36280786)

Peck, A., Carter, S. D. , Mai, H., Chen, S., Burt, A. and Jensen, G. J. (2022) Montage electron tomography of vitrified specimens. Journal of Structural Biology, 214(2), 107860. (doi: 10.1016/j.jsb.2022.107860) (PMID:35487464)

2021

Tran, N.-H., Carter, S. D. , De Mazière, A., Ashkenazi, A., Klumperman, J., Walter, P. and Jensen, G. J. (2021) The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes. Science, 374(6563), pp. 52-57. (doi: 10.1126/science.abh2474) (PMID:34591618)

2020

Zhang, X., Carter, S. D. , Singla, J., White, K. L., Butler, P. C., Stevens, R. C. and Jensen, G. J. (2020) Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography. Science Advances, 6(50), eabc8258. (doi: 10.1126/sciadv.abc8258) (PMID:33298442) (PMCID:PMC7725471)

Kaplan, M., Nicolas, W. J., Xhao, W., Carter, S. D. , Metskas, L. A., Chreifi, G., Ghosal, D. and Jensen, G. J. (2020) In situ imaging and structure determination of biomolecular complexes using electron cryo-tomography. In: Gonen, T. and Nannenga, B. L. (eds.) CryoEM: Methods and Protocols. Series: Methods in molecular biology (2215). Humana: New York, NY, pp. 83-111. ISBN 9781071609651 (doi: 10.1007/978-1-0716-0966-8_4)

Carter, S. D. , Mamede, J. I., Hope, T. J. and Jensen, G. J. (2020) Correlated cryogenic fluorescence microscopy and electron cryo-tomography shows that exogenous TRIM5α can form hexagonal lattices or autophagy aggregates in vivo. Proceedings of the National Academy of Sciences of the United States of America, 117(47), pp. 29702-29711. (doi: 10.1073/pnas.1920323117) (PMID:33154161) (PMCID:PMC7703684)

Carter, S. D. et al. (2020) Ribosome-associated vesicles: a dynamic subcompartment of the endoplasmic reticulum in secretory cells. Science Advances, 6(14), eaay9572. (doi: 10.1126/sciadv.aay9572) (PMID:32270040) (PMCID:PMC7112762)

2019

Azubel, M., Carter, S. D. , Weiszmann, J., Zhang, J., Jensen, G. J., Li, Y. and Kornberh, R. D. (2019) FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles. eLife, 8, e43146. (doi: 10.7554/eLife.43146) (PMID:30688648) (PMCID:PMC6349402)

2018

Siegmund, S. E. et al. (2018) Three-dimensional analysis of mitochondrial crista ultrastructure in a patient with Leigh syndrome by in situ cryoelectron tomography. iScience, 6, pp. 83-91. (doi: 10.1016/j.isci.2018.07.014) (PMID:30240627) (PMCID:PMC6137323)

Carter, S. D. , Mageswaran, S. K., Farino, Z. J., Mamede, J. I., Oikonomou, C. M., Hope, T. J., Freyberg, Z. and Jensen, G. J. (2018) Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells. Journal of Structural Biology, 201(1), pp. 15-26. (doi: 10.1016/j.jsb.2017.10.009) (PMID:29078993) (PMCID:PMC5771259)

2016

Li, Y.-L. et al. (2016) Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. eLife, 5, e16269. (doi: 10.7554/elife.16269) (PMID:27253068) (PMCID:PMC4936896)

2012

Carter, S. D. , Surtees, R., Walter, C. T., Ariza, A., Bergeron, É., Nichol, S. T., Hiscox, J. A., Edwards, T. A. and Barr, J. N. (2012) Structure, function, and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein. Journal of Virology, 86(20), pp. 10914-10923. (doi: 10.1128/JVI.01555-12) (PMID:22875964) (PMCID:PMC3457148)

Carter, S.D. , Barr, J.N. and Edwards, T.A. (2012) Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein. Acta Crystallographica. Section F: Structural Biology Communications, 68(5), pp. 569-573. (doi: 10.1107/s1744309112009736) (PMID:22691790) (PMCID:PMC3374515)

2010

Carter, S. D. et al. (2010) Direct visualization of the small hydrophobic protein of human respiratory syncytial virus reveals the structural basis for membrane permeability. FEBS Letters, 584(13), pp. 2786-2790. (doi: 10.1016/j.febslet.2010.05.006) (PMID:20471980) (PMCID:PMC2896471)

This list was generated on Tue Apr 23 20:54:51 2024 BST.
Jump to: Articles | Book Sections
Number of items: 14.

Articles

Haney, J., Vijayakrishnan, S. , Streetley, J. , Dee, K., Goldfarb, D. M., Clarke, M. , Mullin, M., Carter, S. D. , Bhella, D. and Murcia, P. R. (2022) Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles. Nature Microbiology, 7(11), pp. 1879-1890. (doi: 10.1038/s41564-022-01242-5) (PMID:36280786)

Peck, A., Carter, S. D. , Mai, H., Chen, S., Burt, A. and Jensen, G. J. (2022) Montage electron tomography of vitrified specimens. Journal of Structural Biology, 214(2), 107860. (doi: 10.1016/j.jsb.2022.107860) (PMID:35487464)

Tran, N.-H., Carter, S. D. , De Mazière, A., Ashkenazi, A., Klumperman, J., Walter, P. and Jensen, G. J. (2021) The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes. Science, 374(6563), pp. 52-57. (doi: 10.1126/science.abh2474) (PMID:34591618)

Zhang, X., Carter, S. D. , Singla, J., White, K. L., Butler, P. C., Stevens, R. C. and Jensen, G. J. (2020) Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography. Science Advances, 6(50), eabc8258. (doi: 10.1126/sciadv.abc8258) (PMID:33298442) (PMCID:PMC7725471)

Carter, S. D. , Mamede, J. I., Hope, T. J. and Jensen, G. J. (2020) Correlated cryogenic fluorescence microscopy and electron cryo-tomography shows that exogenous TRIM5α can form hexagonal lattices or autophagy aggregates in vivo. Proceedings of the National Academy of Sciences of the United States of America, 117(47), pp. 29702-29711. (doi: 10.1073/pnas.1920323117) (PMID:33154161) (PMCID:PMC7703684)

Carter, S. D. et al. (2020) Ribosome-associated vesicles: a dynamic subcompartment of the endoplasmic reticulum in secretory cells. Science Advances, 6(14), eaay9572. (doi: 10.1126/sciadv.aay9572) (PMID:32270040) (PMCID:PMC7112762)

Azubel, M., Carter, S. D. , Weiszmann, J., Zhang, J., Jensen, G. J., Li, Y. and Kornberh, R. D. (2019) FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles. eLife, 8, e43146. (doi: 10.7554/eLife.43146) (PMID:30688648) (PMCID:PMC6349402)

Siegmund, S. E. et al. (2018) Three-dimensional analysis of mitochondrial crista ultrastructure in a patient with Leigh syndrome by in situ cryoelectron tomography. iScience, 6, pp. 83-91. (doi: 10.1016/j.isci.2018.07.014) (PMID:30240627) (PMCID:PMC6137323)

Carter, S. D. , Mageswaran, S. K., Farino, Z. J., Mamede, J. I., Oikonomou, C. M., Hope, T. J., Freyberg, Z. and Jensen, G. J. (2018) Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells. Journal of Structural Biology, 201(1), pp. 15-26. (doi: 10.1016/j.jsb.2017.10.009) (PMID:29078993) (PMCID:PMC5771259)

Li, Y.-L. et al. (2016) Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. eLife, 5, e16269. (doi: 10.7554/elife.16269) (PMID:27253068) (PMCID:PMC4936896)

Carter, S. D. , Surtees, R., Walter, C. T., Ariza, A., Bergeron, É., Nichol, S. T., Hiscox, J. A., Edwards, T. A. and Barr, J. N. (2012) Structure, function, and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein. Journal of Virology, 86(20), pp. 10914-10923. (doi: 10.1128/JVI.01555-12) (PMID:22875964) (PMCID:PMC3457148)

Carter, S.D. , Barr, J.N. and Edwards, T.A. (2012) Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein. Acta Crystallographica. Section F: Structural Biology Communications, 68(5), pp. 569-573. (doi: 10.1107/s1744309112009736) (PMID:22691790) (PMCID:PMC3374515)

Carter, S. D. et al. (2010) Direct visualization of the small hydrophobic protein of human respiratory syncytial virus reveals the structural basis for membrane permeability. FEBS Letters, 584(13), pp. 2786-2790. (doi: 10.1016/j.febslet.2010.05.006) (PMID:20471980) (PMCID:PMC2896471)

Book Sections

Kaplan, M., Nicolas, W. J., Xhao, W., Carter, S. D. , Metskas, L. A., Chreifi, G., Ghosal, D. and Jensen, G. J. (2020) In situ imaging and structure determination of biomolecular complexes using electron cryo-tomography. In: Gonen, T. and Nannenga, B. L. (eds.) CryoEM: Methods and Protocols. Series: Methods in molecular biology (2215). Humana: New York, NY, pp. 83-111. ISBN 9781071609651 (doi: 10.1007/978-1-0716-0966-8_4)

This list was generated on Tue Apr 23 20:54:51 2024 BST.

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Using electron cryo-tomography to visualize Bunyavirus infection inside cells
    The Royal Society
    2023 - 2024
     
  • Accelerating throughput at Scotland's national Cryo-EM centre - a next generation direct electron detector for SCMI.
    Medical Research Council
    2022 - 2023