Dr Stephen Carter

Research Fellow (Virology)

Biography

Stephen conducted his postdoctoral research at the California Institute of Technology working with Dr Grant Jensen, a pioneer in the development and application of cryo-electron tomogaphy (cryo-ET). During this time, Stephen developed cryogenic correlated light and electron microscopy (cryo-CLEM) and cryo-ET methods to target and characterise novel specialised compartments of the ER, such as ribosome-associated vesicles (RAVs) and IRE1 oligomers inside ER tubules. Currently, Stephen is pursuing biological projects that require the latest developments in cryo-ET, such as FIB milling and sub-tomogram averaging, and is fostering a deeply collaborative approach to science.

Research interests

The Carter lab is developing tools that will advance cryo-ET including: 1) novel labelling approaches for cryo-CLEM, 2) new cryo-CLEM approaches for visualising organelles inside mammalian cells, 3) and new ways to interpret visual proteomic data. We work closely with collegues in the Scottish Centre for Macromolecular Imaging (SCMI). SCMI is well equipped for cryo-ET sample preparation and data-collection: currently, the facility houses a JEOL CRYO-ARM 300 cryo-EM configured for high-resolution cryo-ET and cryo-CLEM workflows.

Publications

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Number of items: 15.

2024

Martin-Solana, E. et al. (2024) Ribosome-Associated Vesicles promote activity-dependent local translation. bioRxiv, (doi: 10.1101/2024.06.07.598007)

2022

Haney, Joanne, Vijayakrishnan, Swetha ORCID: https://orcid.org/0000-0002-1895-1272, Streetley, James ORCID: https://orcid.org/0000-0001-7808-605X, Dee, Kieran, Goldfarb, Daniel Max, Clarke, Mairi ORCID: https://orcid.org/0000-0002-9658-4308, Mullin, Margaret, Carter, Stephen D. ORCID: https://orcid.org/0000-0002-4237-4276, Bhella, David ORCID: https://orcid.org/0000-0003-2096-8310 and Murcia, Pablo R. ORCID: https://orcid.org/0000-0002-4352-394X (2022) Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles. Nature Microbiology, 7(11), pp. 1879-1890. (doi: 10.1038/s41564-022-01242-5) (PMID:36280786)

Peck, Ariana, Carter, Stephen D. ORCID: https://orcid.org/0000-0002-4237-4276, Mai, Huanghao, Chen, Songye, Burt, Alister and Jensen, Grant J. (2022) Montage electron tomography of vitrified specimens. Journal of Structural Biology, 214(2), 107860. (doi: 10.1016/j.jsb.2022.107860) (PMID:35487464)

2021

Tran, Ngoc-Han, Carter, Stephen D. ORCID: https://orcid.org/0000-0002-4237-4276, De Mazière, Ann, Ashkenazi, Avi, Klumperman, Judith, Walter, Peter and Jensen, Grant J. (2021) The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes. Science, 374(6563), pp. 52-57. (doi: 10.1126/science.abh2474) (PMID:34591618)

2020

Zhang, Xianjun, Carter, Stephen D. ORCID: https://orcid.org/0000-0002-4237-4276, Singla, Jitin, White, Kate L., Butler, Peter C., Stevens, Raymond C. and Jensen, Grant J. (2020) Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography. Science Advances, 6(50), eabc8258. (doi: 10.1126/sciadv.abc8258) (PMID:33298442) (PMCID:PMC7725471)

Kaplan, Mohammed, Nicolas, William J., Xhao, Wei, Carter, Stephen D. ORCID: https://orcid.org/0000-0002-4237-4276, Metskas, Lauren Ann, Chreifi, Georges, Ghosal, Debnath and Jensen, Grant J. (2020) In situ imaging and structure determination of biomolecular complexes using electron cryo-tomography. In: Gonen, Tamir and Nannenga, Brent L. (eds.) CryoEM: Methods and Protocols. Series: Methods in molecular biology (2215). Humana: New York, NY, pp. 83-111. ISBN 9781071609651 (doi: 10.1007/978-1-0716-0966-8_4)

Carter, Stephen D. ORCID: https://orcid.org/0000-0002-4237-4276, Mamede, João I., Hope, Thomas J. and Jensen, Grant J. (2020) Correlated cryogenic fluorescence microscopy and electron cryo-tomography shows that exogenous TRIM5α can form hexagonal lattices or autophagy aggregates in vivo. Proceedings of the National Academy of Sciences of the United States of America, 117(47), pp. 29702-29711. (doi: 10.1073/pnas.1920323117) (PMID:33154161) (PMCID:PMC7703684)

Carter, S. D. et al. (2020) Ribosome-associated vesicles: a dynamic subcompartment of the endoplasmic reticulum in secretory cells. Science Advances, 6(14), eaay9572. (doi: 10.1126/sciadv.aay9572) (PMID:32270040) (PMCID:PMC7112762)

2019

Azubel, Maia, Carter, Stephen D. ORCID: https://orcid.org/0000-0002-4237-4276, Weiszmann, Jennifer, Zhang, Jun, Jensen, Grant J., Li, Yang and Kornberh, Roger D. (2019) FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles. eLife, 8, e43146. (doi: 10.7554/eLife.43146) (PMID:30688648) (PMCID:PMC6349402)

2018

Siegmund, S. E. et al. (2018) Three-dimensional analysis of mitochondrial crista ultrastructure in a patient with Leigh syndrome by in situ cryoelectron tomography. iScience, 6, pp. 83-91. (doi: 10.1016/j.isci.2018.07.014) (PMID:30240627) (PMCID:PMC6137323)

Carter, Stephen D. ORCID: https://orcid.org/0000-0002-4237-4276, Mageswaran, Shrawan K., Farino, Zachary J., Mamede, João I., Oikonomou, Catherine M., Hope, Thomas J., Freyberg, Zachary and Jensen, Grant J. (2018) Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells. Journal of Structural Biology, 201(1), pp. 15-26. (doi: 10.1016/j.jsb.2017.10.009) (PMID:29078993) (PMCID:PMC5771259)

2016

Li, Y.-L. et al. (2016) Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. eLife, 5, e16269. (doi: 10.7554/elife.16269) (PMID:27253068) (PMCID:PMC4936896)

2012

Carter, Stephen D. ORCID: https://orcid.org/0000-0002-4237-4276, Surtees, Rebecca, Walter, Cheryl T., Ariza, Antonio, Bergeron, Éric, Nichol, Stuart T., Hiscox, Julian A., Edwards, Thomas A. and Barr, John N. (2012) Structure, function, and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein. Journal of Virology, 86(20), pp. 10914-10923. (doi: 10.1128/JVI.01555-12) (PMID:22875964) (PMCID:PMC3457148)

Carter, S.D. ORCID: https://orcid.org/0000-0002-4237-4276, Barr, J.N. and Edwards, T.A. (2012) Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein. Acta Crystallographica. Section F: Structural Biology Communications, 68(5), pp. 569-573. (doi: 10.1107/s1744309112009736) (PMID:22691790) (PMCID:PMC3374515)

2010

Carter, S. D. et al. (2010) Direct visualization of the small hydrophobic protein of human respiratory syncytial virus reveals the structural basis for membrane permeability. FEBS Letters, 584(13), pp. 2786-2790. (doi: 10.1016/j.febslet.2010.05.006) (PMID:20471980) (PMCID:PMC2896471)

This list was generated on Thu Oct 30 22:46:32 2025 GMT.

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