Dr Melanie McDonald
- Research Associate (Virology)
telephone:
0141 330 5069
email:
Melanie.McDonald@glasgow.ac.uk
Publications
2025
Reuter, M. et al. (2025) The PAZ domain of Aedes aegypti Dicer 2 is critical for accurate and high-fidelity size determination of virus-derived small interfering RNAs. RNA, 31(5), pp. 679-691. (doi: 10.1261/rna.080149.124) (PMID:39947927) (PMCID:PMC12001973)
Dummunee, Krittika, Parry, Rhys H., Redecke, Lars, Varjak, Margus ORCID: https://orcid.org/0000-0003-2608-5148, Brennan, Benjamin
ORCID: https://orcid.org/0000-0003-4707-726X, Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458 and McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291
(2025)
The catalytic tetrad of Aedes aegypti Argonaute 2 is critical for the antiviral activity of the exogenous siRNA pathway.
Journal of Biological Chemistry, 301(4),
108332.
(doi: 10.1016/j.jbc.2025.108332)
(PMID:39984048)
(PMCID:PMC11968273)
2021
Almire, Floriane, Terhzaz, Selim ORCID: https://orcid.org/0000-0002-6192-6558, Terry, Sandra, McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Gestuveo, Rommel J., Szemiel, Agnieszka M., Varjak, Margus
ORCID: https://orcid.org/0000-0003-2608-5148, McDonald, Alma, Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458 and Pondeville, Emilie
(2021)
Sugar feeding protects against arboviral infection by enhancing gut immunity in the mosquito vector Aedes aegypti.
PLoS Pathogens, 17(9),
e1009870.
(doi: 10.1371/journal.ppat.1009870)
(PMID:34473801)
(PMCID:PMC8412342)
McFarlane, Melanie ORCID: https://orcid.org/0000-0002-8392-2291, Laureti, M., Levée, T., Terry, S., Kohl, A.
ORCID: https://orcid.org/0000-0002-1523-9458 and Pondeville, E.
(2021)
Improved transient silencing of gene expression in the mosquito female Aedes aegypti.
Insect Molecular Biology, 30(3),
pp. 355-365.
(doi: 10.1111/imb.12700)
(PMID:33715239)
2020
McFarlane, M. et al. (2020) The Aedes aegypti Domino orthologue p400 regulates antiviral exogenous small interfering RNA pathway activity and ago-2 expression. mSphere, 5(2), e00081-20. (doi: 10.1128/mSphere.00081-20) (PMID:32269152) (PMCID:PMC7142294)
2017
Dietrich, Isabelle, Shi, Xiaohong ORCID: https://orcid.org/0000-0002-5289-7222, McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Watson, Mick, Blomström, Anne-Lie, Skelton, Jessica K., Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458, Elliott, Richard M. and Schnettler, Esther
(2017)
The antiviral RNAi response in vector and non-vector cells against orthobunya viruses.
PLoS Neglected Tropical Diseases, 11(1),
e0005272.
(doi: 10.1371/journal.pntd.0005272)
(PMID:28060823)
(PMCID:PMC5245901)
2016
Schnettler, Esther, Sreenu, Vattipally B. ORCID: https://orcid.org/0000-0001-6673-4697, Mottram, Timothy and McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291
(2016)
Wolbachia restricts insect specific flavivirus infection in Aedes aegypti cells.
Journal of General Virology, 97(11),
pp. 3024-3029.
(doi: 10.1099/jgv.0.000617)
(PMID:27692043)
(PMCID:PMC5120408)
Wijayanti, Siwi P. M., Porphyre, Thibaud, Chase-Topping, Margo, Rainey, Stephanie M. ORCID: https://orcid.org/0000-0001-9504-5495, McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Schnettler, Esther, Biek, Roman
ORCID: https://orcid.org/0000-0003-3471-5357 and Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458
(2016)
The importance of socio-economic versus environmental risk factors for reported dengue cases in Java, Indonesia.
PLoS Neglected Tropical Diseases, 10(9),
e0004964.
(doi: 10.1371/journal.pntd.0004964)
(PMID:27603137)
(PMCID:PMC5014450)
Rainey, S. M. et al. (2016) Wolbachia blocks viral genome replication early in infection without a transcriptional response by the endosymbiont or host small RNA pathways. PLoS Pathogens, 12(4), e1005536. (doi: 10.1371/journal.ppat.1005536) (PMID:27089431) (PMCID:PMC4835223)
Wijayanti, Siwi P. M., Sunaryo, Sunaryo, Suprihatin, Suprihatin, McFarlane, Melanie ORCID: https://orcid.org/0000-0002-8392-2291, Rainey, Stephanie M.
ORCID: https://orcid.org/0000-0001-9504-5495, Dietrich, Isabelle, Schnettler, Esther, Biek, Roman
ORCID: https://orcid.org/0000-0003-3471-5357 and Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458
(2016)
Dengue in Java, Indonesia: relevance of mosquito indices as risk predictors.
PLoS Neglected Tropical Diseases, 10(3),
e0004500.
(doi: 10.1371/journal.pntd.0004500)
(PMID:26967524)
(PMCID:PMC4788303)
2015
McFarlane, Melanie ORCID: https://orcid.org/0000-0002-8392-2291, MacDonald, Alasdair I., Stevenson, Andrew and Graham, Sheila V.
ORCID: https://orcid.org/0000-0002-7140-8279
(2015)
Human papillomavirus type 16 oncoprotein expression is controlled by the cellular splicing factor SRSF2 (SC35).
Journal of Virology, 89(10),
pp. 5276-5287.
(doi: 10.1128/JVI.03434-14)
(PMID:25717103)
Blomström, A.-L. et al. (2015) Transcriptome analysis reveals the host response to Schmallenberg virus in bovine cells and antagonistic effects of the NSs protein. BMC Genomics, 16, 324. (doi: 10.1186/s12864-015-1538-9) (PMID:25896169) (PMCID:PMC4404599)
Carissimo, G. et al. (2015) Antiviral immunity of Anopheles gambiaeis highly compartmentalized, with distinct roles for RNA interference and gut microbiota. Proceedings of the National Academy of Sciences of the United States of America, 112(2), E176-E185. (doi: 10.1073/pnas.1412984112) (PMID:25548172) (PMCID:PMC4299212)
Kean, Joy, Rainey, Stephanie ORCID: https://orcid.org/0000-0001-9504-5495, McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Donald, Claire
ORCID: https://orcid.org/0000-0002-4370-0707, Schnettler, Esther, Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458 and Pondeville, Emilie
(2015)
Fighting arbovirus transmission: natural and engineered control of vector competence in aedes mosquitoes.
Insects, 6(1),
pp. 236-278.
(doi: 10.3390/insects6010236)
(PMID:26463078)
(PMCID:PMC4553541)
2014
McFarlane, M. et al. (2014) Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication. PLoS Neglected Tropical Diseases, 8(7), e2994. (doi: 10.1371/journal.pntd.0002994) (PMID:25058001) (PMCID:PMC4109886)
Schnettler, E. et al. (2014) Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses. Nucleic Acids Research, 42(14), pp. 9436-9446. (doi: 10.1093/nar/gku657)
2013
Schnettler, Esther, Donald, Claire L. ORCID: https://orcid.org/0000-0002-4370-0707, Human, Stacey, Watson, Mick, Siu, Ricky W. C., McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Fazakerley, John K., Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458 and Fragkoudis, Rennos
(2013)
Knockdown of piRNA pathway proteins results in enhanced Semliki forest virus production in mosquito cells.
Journal of General Virology, 94(Pt. 7),
pp. 1680-1689.
(doi: 10.1099/vir.0.053850-0)
(PMID:23559478)
(PMCID:PMC3709635)
Schnettler, Esther, Ratinier, Maxime, Watson, Mick, Shaw, Andrew E., McFarlane, Melanie, Varela, Mariana, Elliott, Richard M., Palmarini, Massimo ORCID: https://orcid.org/0000-0001-7007-4070 and Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458
(2013)
RNA interference targets arbovirus replication in Culicoides cells.
Journal of Virology, 87(5),
pp. 2441-2454.
(doi: 10.1128/JVI.02848-12)
(PMID:23269795)
(PMCID:PMC3571378)
Varela, M. et al. (2013) Schmallenberg virus pathogenesis, tropism and interaction with the innate immune system of the host. PLoS Pathogens, 9(1), e1003133. (doi: 10.1371/journal.ppat.1003133) (PMID:23326235) (PMCID:PMC3542112)
2010
McFarlane, Melanie ORCID: https://orcid.org/0000-0002-8392-2291 and Graham, S.V.
ORCID: https://orcid.org/0000-0002-7140-8279
(2010)
Human papillomavirus regulation of SR proteins.
Biochemical Society Transactions, 38(4),
pp. 1116-1121.
(doi: 10.1042/BST0381116)
2009
Mole, S., McFarlane, M. ORCID: https://orcid.org/0000-0002-8392-2291, Chuen-Im, T., Milligan, S.G., Millan, D. and Graham, S.V.
(2009)
RNA splicing factors regulated by HPV16 during cervical tumour progression.
Journal of Pathology, 219(3),
pp. 383-391.
(doi: 10.1002/path.2608)
(PMID:19718710)
(PMCID:PMC2779514)
Articles
Reuter, M. et al. (2025) The PAZ domain of Aedes aegypti Dicer 2 is critical for accurate and high-fidelity size determination of virus-derived small interfering RNAs. RNA, 31(5), pp. 679-691. (doi: 10.1261/rna.080149.124) (PMID:39947927) (PMCID:PMC12001973)
Dummunee, Krittika, Parry, Rhys H., Redecke, Lars, Varjak, Margus ORCID: https://orcid.org/0000-0003-2608-5148, Brennan, Benjamin
ORCID: https://orcid.org/0000-0003-4707-726X, Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458 and McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291
(2025)
The catalytic tetrad of Aedes aegypti Argonaute 2 is critical for the antiviral activity of the exogenous siRNA pathway.
Journal of Biological Chemistry, 301(4),
108332.
(doi: 10.1016/j.jbc.2025.108332)
(PMID:39984048)
(PMCID:PMC11968273)
Almire, Floriane, Terhzaz, Selim ORCID: https://orcid.org/0000-0002-6192-6558, Terry, Sandra, McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Gestuveo, Rommel J., Szemiel, Agnieszka M., Varjak, Margus
ORCID: https://orcid.org/0000-0003-2608-5148, McDonald, Alma, Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458 and Pondeville, Emilie
(2021)
Sugar feeding protects against arboviral infection by enhancing gut immunity in the mosquito vector Aedes aegypti.
PLoS Pathogens, 17(9),
e1009870.
(doi: 10.1371/journal.ppat.1009870)
(PMID:34473801)
(PMCID:PMC8412342)
McFarlane, Melanie ORCID: https://orcid.org/0000-0002-8392-2291, Laureti, M., Levée, T., Terry, S., Kohl, A.
ORCID: https://orcid.org/0000-0002-1523-9458 and Pondeville, E.
(2021)
Improved transient silencing of gene expression in the mosquito female Aedes aegypti.
Insect Molecular Biology, 30(3),
pp. 355-365.
(doi: 10.1111/imb.12700)
(PMID:33715239)
McFarlane, M. et al. (2020) The Aedes aegypti Domino orthologue p400 regulates antiviral exogenous small interfering RNA pathway activity and ago-2 expression. mSphere, 5(2), e00081-20. (doi: 10.1128/mSphere.00081-20) (PMID:32269152) (PMCID:PMC7142294)
Dietrich, Isabelle, Shi, Xiaohong ORCID: https://orcid.org/0000-0002-5289-7222, McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Watson, Mick, Blomström, Anne-Lie, Skelton, Jessica K., Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458, Elliott, Richard M. and Schnettler, Esther
(2017)
The antiviral RNAi response in vector and non-vector cells against orthobunya viruses.
PLoS Neglected Tropical Diseases, 11(1),
e0005272.
(doi: 10.1371/journal.pntd.0005272)
(PMID:28060823)
(PMCID:PMC5245901)
Schnettler, Esther, Sreenu, Vattipally B. ORCID: https://orcid.org/0000-0001-6673-4697, Mottram, Timothy and McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291
(2016)
Wolbachia restricts insect specific flavivirus infection in Aedes aegypti cells.
Journal of General Virology, 97(11),
pp. 3024-3029.
(doi: 10.1099/jgv.0.000617)
(PMID:27692043)
(PMCID:PMC5120408)
Wijayanti, Siwi P. M., Porphyre, Thibaud, Chase-Topping, Margo, Rainey, Stephanie M. ORCID: https://orcid.org/0000-0001-9504-5495, McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Schnettler, Esther, Biek, Roman
ORCID: https://orcid.org/0000-0003-3471-5357 and Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458
(2016)
The importance of socio-economic versus environmental risk factors for reported dengue cases in Java, Indonesia.
PLoS Neglected Tropical Diseases, 10(9),
e0004964.
(doi: 10.1371/journal.pntd.0004964)
(PMID:27603137)
(PMCID:PMC5014450)
Rainey, S. M. et al. (2016) Wolbachia blocks viral genome replication early in infection without a transcriptional response by the endosymbiont or host small RNA pathways. PLoS Pathogens, 12(4), e1005536. (doi: 10.1371/journal.ppat.1005536) (PMID:27089431) (PMCID:PMC4835223)
Wijayanti, Siwi P. M., Sunaryo, Sunaryo, Suprihatin, Suprihatin, McFarlane, Melanie ORCID: https://orcid.org/0000-0002-8392-2291, Rainey, Stephanie M.
ORCID: https://orcid.org/0000-0001-9504-5495, Dietrich, Isabelle, Schnettler, Esther, Biek, Roman
ORCID: https://orcid.org/0000-0003-3471-5357 and Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458
(2016)
Dengue in Java, Indonesia: relevance of mosquito indices as risk predictors.
PLoS Neglected Tropical Diseases, 10(3),
e0004500.
(doi: 10.1371/journal.pntd.0004500)
(PMID:26967524)
(PMCID:PMC4788303)
McFarlane, Melanie ORCID: https://orcid.org/0000-0002-8392-2291, MacDonald, Alasdair I., Stevenson, Andrew and Graham, Sheila V.
ORCID: https://orcid.org/0000-0002-7140-8279
(2015)
Human papillomavirus type 16 oncoprotein expression is controlled by the cellular splicing factor SRSF2 (SC35).
Journal of Virology, 89(10),
pp. 5276-5287.
(doi: 10.1128/JVI.03434-14)
(PMID:25717103)
Blomström, A.-L. et al. (2015) Transcriptome analysis reveals the host response to Schmallenberg virus in bovine cells and antagonistic effects of the NSs protein. BMC Genomics, 16, 324. (doi: 10.1186/s12864-015-1538-9) (PMID:25896169) (PMCID:PMC4404599)
Carissimo, G. et al. (2015) Antiviral immunity of Anopheles gambiaeis highly compartmentalized, with distinct roles for RNA interference and gut microbiota. Proceedings of the National Academy of Sciences of the United States of America, 112(2), E176-E185. (doi: 10.1073/pnas.1412984112) (PMID:25548172) (PMCID:PMC4299212)
Kean, Joy, Rainey, Stephanie ORCID: https://orcid.org/0000-0001-9504-5495, McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Donald, Claire
ORCID: https://orcid.org/0000-0002-4370-0707, Schnettler, Esther, Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458 and Pondeville, Emilie
(2015)
Fighting arbovirus transmission: natural and engineered control of vector competence in aedes mosquitoes.
Insects, 6(1),
pp. 236-278.
(doi: 10.3390/insects6010236)
(PMID:26463078)
(PMCID:PMC4553541)
McFarlane, M. et al. (2014) Characterization of Aedes aegypti innate-immune pathways that limit Chikungunya virus replication. PLoS Neglected Tropical Diseases, 8(7), e2994. (doi: 10.1371/journal.pntd.0002994) (PMID:25058001) (PMCID:PMC4109886)
Schnettler, E. et al. (2014) Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses. Nucleic Acids Research, 42(14), pp. 9436-9446. (doi: 10.1093/nar/gku657)
Schnettler, Esther, Donald, Claire L. ORCID: https://orcid.org/0000-0002-4370-0707, Human, Stacey, Watson, Mick, Siu, Ricky W. C., McFarlane, Melanie
ORCID: https://orcid.org/0000-0002-8392-2291, Fazakerley, John K., Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458 and Fragkoudis, Rennos
(2013)
Knockdown of piRNA pathway proteins results in enhanced Semliki forest virus production in mosquito cells.
Journal of General Virology, 94(Pt. 7),
pp. 1680-1689.
(doi: 10.1099/vir.0.053850-0)
(PMID:23559478)
(PMCID:PMC3709635)
Schnettler, Esther, Ratinier, Maxime, Watson, Mick, Shaw, Andrew E., McFarlane, Melanie, Varela, Mariana, Elliott, Richard M., Palmarini, Massimo ORCID: https://orcid.org/0000-0001-7007-4070 and Kohl, Alain
ORCID: https://orcid.org/0000-0002-1523-9458
(2013)
RNA interference targets arbovirus replication in Culicoides cells.
Journal of Virology, 87(5),
pp. 2441-2454.
(doi: 10.1128/JVI.02848-12)
(PMID:23269795)
(PMCID:PMC3571378)
Varela, M. et al. (2013) Schmallenberg virus pathogenesis, tropism and interaction with the innate immune system of the host. PLoS Pathogens, 9(1), e1003133. (doi: 10.1371/journal.ppat.1003133) (PMID:23326235) (PMCID:PMC3542112)
McFarlane, Melanie ORCID: https://orcid.org/0000-0002-8392-2291 and Graham, S.V.
ORCID: https://orcid.org/0000-0002-7140-8279
(2010)
Human papillomavirus regulation of SR proteins.
Biochemical Society Transactions, 38(4),
pp. 1116-1121.
(doi: 10.1042/BST0381116)
Mole, S., McFarlane, M. ORCID: https://orcid.org/0000-0002-8392-2291, Chuen-Im, T., Milligan, S.G., Millan, D. and Graham, S.V.
(2009)
RNA splicing factors regulated by HPV16 during cervical tumour progression.
Journal of Pathology, 219(3),
pp. 383-391.
(doi: 10.1002/path.2608)
(PMID:19718710)
(PMCID:PMC2779514)
Research datasets
2025
Dummunee, K., Parry, R. H., Redecke, L., Varjak, M., Brennan, B. , Kohl, A. and McFarlane, M. (2025) The slicer activity of Aedes aegypti Argonaute 2 is critical for the antiviral activity of the antiviral exogenous siRNA pathway. [Data Collection]
2021
McFarlane, M. , Laureti, M., Levée, T., Terry, S., Kohl, A. and Pondeville, E. (2021) Improved transient silencing of gene expression in the mosquito female Aedes aegypti. [Data Collection]
2020
Mcdonald, M. , Pondeville, E. and Kohl, A. (2020) The Aedes aegypti Domino ortholog, p400 regulates antiviral exogenous siRNA pathway activity through control of ago-2 levels. [Data Collection]