Ms Diane Vaughan

  • Flow Cytometry Core Facility Manager (Infection & Immunity Professional Services)

telephone: 01413302626
email: Diane.Vaughan@glasgow.ac.uk

The iii- Flow Core Facility, B4/44, Glasgow Biomedical Research Centre, 120 University Place, Glasgow, G12 8TA

ORCID iDhttps://orcid.org/0000-0002-4530-2278

Biography

I am a highly experienced research technological professional, with a background in medical microbiology and 23 years of experience in Immunology. The last 12 years have been spent establishing and building the reputation of the Flow Cytometry Core Facility in the School of Infection & Immunity which I manage.

My specialist knowledge of flow cytometry and its application to immune monitoring and biomarker discovery in translational research has been critical in serving a large research community with diverse scientific needs. I have also had direct responsibility for managing junior staff, training and supervising junior scientists. In addition to this my teaching commitments include lectures for both undergraduate and postgraduate courses at Glasgow.

Whilst I have authorship on relatively few papers I have made a significant contribution to the Cytometry sections of 55 research papers since 2019

Key Skills

Flow Cytometry and Cell sorting

Full Spectrum Cytometry

High Dimensional Data Analysis

Luminex Technology

CBA & Legendplex Assay

Method optimisation and sample preparation 

Panel design and optimisation

Experimental planning & post acquisition data analysis

Biosafety and lab management

Practical & Theoretical Cytometry Training

Imaging Cytometry

 

 

Publications

List by: Type | Date

Jump to: 2019 | 2018 | 2017 | 2016 | 2014 | 2012 | 2001
Number of items: 12.

2019

Elmesmari, A. et al. (2019) Do Functional Interactions Between Macrophages and Synovial Fibroblasts Drive Synovial Pathology or Resolution of Inflammation? European League Against Rheumatism (EULAR) 2019 Congress, Madrid, Spain, 12-15 Jun 2019.

2018

Elmesmari, A., Alivernini, S., Tolusso, B., Bui, L., Vaughan, D. , Federico, F., Gremese, E., McInnes, I. B. and Kurowska-Stolarska, M. (2018) Stolarska Synovial Tissue CD1c+ Dendritic Cells in Rheumatoid Arthritis Express High Levels of the Epigenetic Regulator of Inflammation, Microrna-155 and Inflammatory Cytokines. EULAR 2018: Annual European Congress of Rheumatology, Amsterdam, Netherlands, 13-16 June 2018. (Unpublished)

Elmesmari, A., Alivernini, S., Tolusso, B., Vaughan, D. , Petricca, L., Gremese, E., Ferraccioli, G., McInnes, I. B. and Kurowska-Stolarska, M. (2018) Synovial Tissue of RA Patients in Remission Contains a Unique Population of Regulatory Macrophages. Microphage Biology in the Single-Cell Era, Ghent, Belgium, 2018. (Unpublished)

2017

Elmesmari, A., Alivernini, S., Tolusso, B., Vaughan, D. , Petricca, L., Gremese, E., Ferraccioli, G., McInnes, I. B. and Kurowska-Stolarska, M. (2017) Synovial Tissue of RA Patients in Remission Contains a Unique Population of Regulatory Macrophages. EULAR 2017: Annual European Congress of Rheumatology, Madrid, Spain, 14-17 June 2017. (Unpublished)

Elmesmari, A., Alivernini, S., Vaughan, D. , Ferraccioli, G., McInnes, I. B. and Kurowska-Stolarska, M. (2017) Characterisation of Synovium Tissues Macropahges. Rheumatology 2017, Birmingham, UK, 25-27 April 2017. (Unpublished)

Elmesmari, A., Alivernini, S., Tolusso, B., Vaughan, D. , Petricca, L., Gremese, E., Ferraccioli, G., McInnes, I. B. and Kurowska-Stolarska, M. (2017) Synovial Tissue of RA Patients in Remission Contains a Unique Population of Regulatory Macrophages. 37th European Workshop for Rheumatology Research (EWRR 2017), Athens, Greece, 2-4 March 2017. (Unpublished)

2016

Elmesmari, A., Fraser, A. R., Wood, C., Gilchrist, D., Vaughan, D. , Stewart, L., McSharry, C., McInnes, I. B. and Kurowska-Stolarska, M. (2016) MicroRNA-155 regulates monocyte chemokine and chemokine receptor expression in Rheumatoid Arthritis. Rheumatology, 55(11), pp. 2056-2065. (doi: 10.1093/rheumatology/kew272) (PMID:27411480) (PMCID:PMC5088623)

2014

Komai-Koma, M., Li, D., Wang, E., Vaughan, D. and Xu, D. (2014) Anti-Toll-like receptor 2 and 4 antibodies suppress inflammatory response in mice. Immunology, 143(3), pp. 354-362. (doi: 10.1111/imm.12312)

Elmesmari, A., Gilchrist, D. S., Fraser, A. R., Vaughan, D. , Graham, G. J. , Brewer, J. , Kurowska-Stolarska, M. and McInnes, I. B. (2014) MicroRNA-155 Regulates Chemokines and Chemokine Receptors in Rheumatoid Arthritis Monocytes. Libya Higher Education Forum, London, UK, 5-6 June 2014. (Unpublished)

2012

Tiengwe, C. et al. (2012) Genome-wide analysis reveals extensive functional interaction between DNA replication initiation and transcription in the genome of trypanosoma brucei. Cell Reports, 2(1), pp. 185-197. (doi: 10.1016/j.celrep.2012.06.007)

Elmesmari, A., Gilchrist, D. S., Fraser, A. D., Vaughan, D. , McQueenie, R., Graham, G. , Brewer, J. , McInnes, I. B. and Kurowska-Stolarska, M. (2012) Microrna-155 regulates chemokines and chemokine receptors in Rheumatoid Arthritis monocyte. In: American College of Rheumatology & Association of Rheumatology Health Professionals, Annual Scientific Meeting, Washington DC, USA, 9-14 Nov 2012, S384. (doi: 10.1002/art.37735)

2001

Trif, M., Guillen, C., Vaughan, D. M. , Telfer, J. M., Brewer, J. M. , Roseanu, A. and Brock, J. H. (2001) Liposomes as possible carriers for lactoferrin in the local treatment of inflammatory diseases. Experimental Biology and Medicine, 226(6), pp. 559-564. (doi: 10.1177/153537020122600608) (PMID:11395926)

This list was generated on Mon May 29 15:31:46 2023 BST.
Jump to: Articles | Conference or Workshop Item | Conference Proceedings
Number of items: 12.

Articles

Elmesmari, A., Fraser, A. R., Wood, C., Gilchrist, D., Vaughan, D. , Stewart, L., McSharry, C., McInnes, I. B. and Kurowska-Stolarska, M. (2016) MicroRNA-155 regulates monocyte chemokine and chemokine receptor expression in Rheumatoid Arthritis. Rheumatology, 55(11), pp. 2056-2065. (doi: 10.1093/rheumatology/kew272) (PMID:27411480) (PMCID:PMC5088623)

Komai-Koma, M., Li, D., Wang, E., Vaughan, D. and Xu, D. (2014) Anti-Toll-like receptor 2 and 4 antibodies suppress inflammatory response in mice. Immunology, 143(3), pp. 354-362. (doi: 10.1111/imm.12312)

Tiengwe, C. et al. (2012) Genome-wide analysis reveals extensive functional interaction between DNA replication initiation and transcription in the genome of trypanosoma brucei. Cell Reports, 2(1), pp. 185-197. (doi: 10.1016/j.celrep.2012.06.007)

Trif, M., Guillen, C., Vaughan, D. M. , Telfer, J. M., Brewer, J. M. , Roseanu, A. and Brock, J. H. (2001) Liposomes as possible carriers for lactoferrin in the local treatment of inflammatory diseases. Experimental Biology and Medicine, 226(6), pp. 559-564. (doi: 10.1177/153537020122600608) (PMID:11395926)

Conference or Workshop Item

Elmesmari, A. et al. (2019) Do Functional Interactions Between Macrophages and Synovial Fibroblasts Drive Synovial Pathology or Resolution of Inflammation? European League Against Rheumatism (EULAR) 2019 Congress, Madrid, Spain, 12-15 Jun 2019.

Elmesmari, A., Alivernini, S., Tolusso, B., Bui, L., Vaughan, D. , Federico, F., Gremese, E., McInnes, I. B. and Kurowska-Stolarska, M. (2018) Stolarska Synovial Tissue CD1c+ Dendritic Cells in Rheumatoid Arthritis Express High Levels of the Epigenetic Regulator of Inflammation, Microrna-155 and Inflammatory Cytokines. EULAR 2018: Annual European Congress of Rheumatology, Amsterdam, Netherlands, 13-16 June 2018. (Unpublished)

Elmesmari, A., Alivernini, S., Tolusso, B., Vaughan, D. , Petricca, L., Gremese, E., Ferraccioli, G., McInnes, I. B. and Kurowska-Stolarska, M. (2018) Synovial Tissue of RA Patients in Remission Contains a Unique Population of Regulatory Macrophages. Microphage Biology in the Single-Cell Era, Ghent, Belgium, 2018. (Unpublished)

Elmesmari, A., Alivernini, S., Tolusso, B., Vaughan, D. , Petricca, L., Gremese, E., Ferraccioli, G., McInnes, I. B. and Kurowska-Stolarska, M. (2017) Synovial Tissue of RA Patients in Remission Contains a Unique Population of Regulatory Macrophages. EULAR 2017: Annual European Congress of Rheumatology, Madrid, Spain, 14-17 June 2017. (Unpublished)

Elmesmari, A., Alivernini, S., Vaughan, D. , Ferraccioli, G., McInnes, I. B. and Kurowska-Stolarska, M. (2017) Characterisation of Synovium Tissues Macropahges. Rheumatology 2017, Birmingham, UK, 25-27 April 2017. (Unpublished)

Elmesmari, A., Alivernini, S., Tolusso, B., Vaughan, D. , Petricca, L., Gremese, E., Ferraccioli, G., McInnes, I. B. and Kurowska-Stolarska, M. (2017) Synovial Tissue of RA Patients in Remission Contains a Unique Population of Regulatory Macrophages. 37th European Workshop for Rheumatology Research (EWRR 2017), Athens, Greece, 2-4 March 2017. (Unpublished)

Elmesmari, A., Gilchrist, D. S., Fraser, A. R., Vaughan, D. , Graham, G. J. , Brewer, J. , Kurowska-Stolarska, M. and McInnes, I. B. (2014) MicroRNA-155 Regulates Chemokines and Chemokine Receptors in Rheumatoid Arthritis Monocytes. Libya Higher Education Forum, London, UK, 5-6 June 2014. (Unpublished)

Conference Proceedings

Elmesmari, A., Gilchrist, D. S., Fraser, A. D., Vaughan, D. , McQueenie, R., Graham, G. , Brewer, J. , McInnes, I. B. and Kurowska-Stolarska, M. (2012) Microrna-155 regulates chemokines and chemokine receptors in Rheumatoid Arthritis monocyte. In: American College of Rheumatology & Association of Rheumatology Health Professionals, Annual Scientific Meeting, Washington DC, USA, 9-14 Nov 2012, S384. (doi: 10.1002/art.37735)

This list was generated on Tue May 30 15:33:42 2023 BST.

Teaching

Sii - Animal Models of Disease (Masters): Flow Cytometry, Imaging & Cytomics

Sii - Immunology 3A & 3B (Undergraduate): Cytometry Theory

The Scottish Society for Cytomics: The Fundamentals of Flow Cytometry Course (in conjunction with fellow Scottish Cytometrists)

Professional activities & recognition

Professional & learned societies

  • 2014 - present: Member of steering commitee, SSC (Scottish Society for Cytomics)
  • 2010 - present: Member, FCUK (Flow Cytometry UK)