Dr Jesko Koehnke

  • Reader (School of Chemistry)

Research interests

Research Interests

Microbial natural products are an invaluable source of novel bioactive molecules. The Koehnke group focusses on elucidating biosynthetic steps from selected natural product pathways through a combination of biochemistry, biophysics, and structural biology. We then apply these data to generate (semisynthetic) natural product derivatives with improved properties or to derivatize (semi)synthetic molecules for drug discovery.

In addition, we collaborate with diverse groups, mainly as structural biologists, to understand biological problems, such as antibiotic resistance development or the infection process and to aid in drug design and optimization. 

Research Topics

Natural product biosynthesis and derivatization

Enzymatic reaction mechanisms

Host-pathogen interactions

Natural product discovery

 

Publication Highlights

  1. Sikandar A, Franz L, Melse O, Antes I, Köhnke J (2019): Thiazoline-Specific Amidohydrolase PurAH Is the Gatekeeper of Bottromycin Biosynthesis. J Am Chem Soc. doi 10.1021/jacs.8b12231
  2. Sikandar A, Cirnski K, Testolin G, Volz C, Brönstrup M, Kalinina OV, Müller R, Köhnke J (2018): Adaptation of a Bacterial Multidrug Resistance System Revealed by the Structure and Function of AlbA. J Am Chem Soc. 140(48), pp. 16641-49.
  3. Franz L, Adam S, Santos-Aberturas J, Truman AW, Köhnke J (2017): Macroamidine formation in bottromycins is catalyzed by a divergent YcaO enzyme. J Am Chem Soc. 139(50), pp. 18158-61.
  4. Fu C, Sikandar A, Donner J, Zaburannyi N, Herrmann J, Reck M, Wagner-Döbler I+, Köhnke J+, Müller R+ (2017): The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD. Nat Comm, 8(1): 1529
  5. Köhnke J, Mann G, Bent AF, Ludewig H, Shirran S, Botting C, Lebl T, Houssen WE, Jaspars M, Naismith JH (2015): Structural analysis of leader-peptide binding enables leader-free cyanobactin processing. Nat Chem Biol., 11(8), pp. 558-63.

Publications

List by: Type | Date

Jump to: 2020 | 2019 | 2018 | 2017 | 2015
Number of items: 12.

2020

Adam, S., Franz, L., Milhim, M., Bernhardt, R., Kalinina, O. V. and Koehnke, J. (2020) Characterization of the stereoselective P450 enzyme BotCYP enables the in vitro biosynthesis of the Bottromycin Core Scaffold. Journal of the American Chemical Society, 142(49), pp. 20560-20565. (doi: 10.1021/jacs.0c10361)

Axer, A., Jumde, R. P., Adam, S., Faust, A., Schäfers, M., Fobker, M., Koehnke, J. , Hirsch, A. K. H. and Gilmour, R. (2020) Enhancing glycan stability via site-selective fluorination: modulating substrate orientation by molecular design. Chemical Science, (doi: 10.1039/D0SC04297H) (Early Online Publication)

Sikandar, A., Franz, L., Adam, S., Santos-Aberturas, J., Horbal, L., Luzhetskyy, A., Truman, A. W., Kalinina, O. V. and Koehnke, J. (2020) The bottromycin epimerase BotH defines a group of atypical α/β-hydrolase-fold enzymes. Nature Chemical Biology, 16(9), pp. 1013-1018. (doi: 10.1038/s41589-020-0569-y) (PMID:32601484)

Keller, L. et al. (2020) Tutuilamides A–C: vinyl-chloride-containing cyclodepsipeptides from marine cyanobacteria with potent elastase inhibitory properties. ACS Chemical Biology, 15(3), pp. 751-757. (doi: 10.1021/acschembio.9b00992) (PMID:31935054)

2019

Sikandar, A., Franz, L., Melse, O., Antes, I. and Koehnke, J. (2019) Thiazoline-specific amidohydrolase PurAH is the gatekeeper of bottromycin biosynthesis. Journal of the American Chemical Society, 141(25), pp. 9748-9752. (doi: 10.1021/jacs.8b12231) (PMID:31192589)

2018

Sikandar, A., Cirnski, K., Testolin, G., Volz, C., Brönstrup, M., Kalinina, O. V., Müller, R. and Koehnke, J. (2018) Adaptation of a bacterial multidrug resistance system revealed by the structure and function of AlbA. Journal of the American Chemical Society, 140(48), pp. 16641-16649. (doi: 10.1021/jacs.8b08895) (PMID:30422653)

Kany, A. M., Sikandar, A., Yahiaoui, S., Haupenthal, J., Walter, I., Empting, M., Köhnke, J. and Hartmann, R. W. (2018) Tackling pseudomonas aeruginosa virulence by a hydroxamic acid-absed LasB inhibitor. ACS Chemical Biology, 13(9), pp. 2449-2455. (doi: 10.1021/acschembio.8b00257) (PMID:30088919)

2017

Franz, L., Adam, S., Santos-Aberturas, J., Truman, A. W. and Koehnke, J. (2017) Macroamidine formation in bottromycins is catalyzed by a divergent YcaO enzyme. Journal of the American Chemical Society, 139(50), pp. 18158-18161. (doi: 10.1021/jacs.7b09898) (PMID:29206037)

Kjaerulff, L., Sikandar, A., Zaburannyi, N., Adam, S., Herrmann, J., Koehnke, J. and Müller, R. (2017) Thioholgamides: thioamide-containing cytotoxic RiPP natural products. ACS Chemical Biology, 12(11), pp. 2837-2841. (doi: 10.1021/acschembio.7b00676) (PMID:28981254)

Fu, C., Sikandar, A., Donner, J., Zaburannyi, N., Herrmann, J., Reck, M., Wagner-Döbler, I., Koehnke, J. and Müller, R. (2017) The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD. Nature Communications, 8(1), 1529. (doi: 10.1038/s41467-017-01671-5) (PMID:29142318) (PMCID:29142318)

Viehrig, K., Surup, F., Volz, C., Herrmann, J., Abou Fayad, A., Adam, S., Köhnke, J. , Trauner, D. and Müller, R. (2017) Structure and biosynthesis of crocagins: polycyclic posttranslationally modified ribosomal peptides from chondromyces crocatus. Angewandte Chemie (International Edition), 56(26), pp. 7407-7410. (doi: 10.1002/anie.201612640) (PMID:28544148)

2015

Koehnke, J. , Mann, G., Bent, A. F., Ludewig, H., Shirran, S., Botting, C., Lebl, T., Houssen, W. E., Jaspars, M. and Naismith, J. H. (2015) Structural analysis of leader peptide binding enables leader-free cyanobactin processing. Nature Chemical Biology, 11(8), pp. 558-563. (doi: 10.1038/nchembio.1841) (PMID:26098679) (PMCID:PMC4512242)

This list was generated on Tue Jan 19 23:04:47 2021 GMT.
Jump to: Articles
Number of items: 12.

Articles

Adam, S., Franz, L., Milhim, M., Bernhardt, R., Kalinina, O. V. and Koehnke, J. (2020) Characterization of the stereoselective P450 enzyme BotCYP enables the in vitro biosynthesis of the Bottromycin Core Scaffold. Journal of the American Chemical Society, 142(49), pp. 20560-20565. (doi: 10.1021/jacs.0c10361)

Axer, A., Jumde, R. P., Adam, S., Faust, A., Schäfers, M., Fobker, M., Koehnke, J. , Hirsch, A. K. H. and Gilmour, R. (2020) Enhancing glycan stability via site-selective fluorination: modulating substrate orientation by molecular design. Chemical Science, (doi: 10.1039/D0SC04297H) (Early Online Publication)

Sikandar, A., Franz, L., Adam, S., Santos-Aberturas, J., Horbal, L., Luzhetskyy, A., Truman, A. W., Kalinina, O. V. and Koehnke, J. (2020) The bottromycin epimerase BotH defines a group of atypical α/β-hydrolase-fold enzymes. Nature Chemical Biology, 16(9), pp. 1013-1018. (doi: 10.1038/s41589-020-0569-y) (PMID:32601484)

Keller, L. et al. (2020) Tutuilamides A–C: vinyl-chloride-containing cyclodepsipeptides from marine cyanobacteria with potent elastase inhibitory properties. ACS Chemical Biology, 15(3), pp. 751-757. (doi: 10.1021/acschembio.9b00992) (PMID:31935054)

Sikandar, A., Franz, L., Melse, O., Antes, I. and Koehnke, J. (2019) Thiazoline-specific amidohydrolase PurAH is the gatekeeper of bottromycin biosynthesis. Journal of the American Chemical Society, 141(25), pp. 9748-9752. (doi: 10.1021/jacs.8b12231) (PMID:31192589)

Sikandar, A., Cirnski, K., Testolin, G., Volz, C., Brönstrup, M., Kalinina, O. V., Müller, R. and Koehnke, J. (2018) Adaptation of a bacterial multidrug resistance system revealed by the structure and function of AlbA. Journal of the American Chemical Society, 140(48), pp. 16641-16649. (doi: 10.1021/jacs.8b08895) (PMID:30422653)

Kany, A. M., Sikandar, A., Yahiaoui, S., Haupenthal, J., Walter, I., Empting, M., Köhnke, J. and Hartmann, R. W. (2018) Tackling pseudomonas aeruginosa virulence by a hydroxamic acid-absed LasB inhibitor. ACS Chemical Biology, 13(9), pp. 2449-2455. (doi: 10.1021/acschembio.8b00257) (PMID:30088919)

Franz, L., Adam, S., Santos-Aberturas, J., Truman, A. W. and Koehnke, J. (2017) Macroamidine formation in bottromycins is catalyzed by a divergent YcaO enzyme. Journal of the American Chemical Society, 139(50), pp. 18158-18161. (doi: 10.1021/jacs.7b09898) (PMID:29206037)

Kjaerulff, L., Sikandar, A., Zaburannyi, N., Adam, S., Herrmann, J., Koehnke, J. and Müller, R. (2017) Thioholgamides: thioamide-containing cytotoxic RiPP natural products. ACS Chemical Biology, 12(11), pp. 2837-2841. (doi: 10.1021/acschembio.7b00676) (PMID:28981254)

Fu, C., Sikandar, A., Donner, J., Zaburannyi, N., Herrmann, J., Reck, M., Wagner-Döbler, I., Koehnke, J. and Müller, R. (2017) The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD. Nature Communications, 8(1), 1529. (doi: 10.1038/s41467-017-01671-5) (PMID:29142318) (PMCID:29142318)

Viehrig, K., Surup, F., Volz, C., Herrmann, J., Abou Fayad, A., Adam, S., Köhnke, J. , Trauner, D. and Müller, R. (2017) Structure and biosynthesis of crocagins: polycyclic posttranslationally modified ribosomal peptides from chondromyces crocatus. Angewandte Chemie (International Edition), 56(26), pp. 7407-7410. (doi: 10.1002/anie.201612640) (PMID:28544148)

Koehnke, J. , Mann, G., Bent, A. F., Ludewig, H., Shirran, S., Botting, C., Lebl, T., Houssen, W. E., Jaspars, M. and Naismith, J. H. (2015) Structural analysis of leader peptide binding enables leader-free cyanobactin processing. Nature Chemical Biology, 11(8), pp. 558-563. (doi: 10.1038/nchembio.1841) (PMID:26098679) (PMCID:PMC4512242)

This list was generated on Tue Jan 19 23:04:47 2021 GMT.