Dr Lilach Sheiner

  • Senior Lecturer and Royal Society of Edinburgh Personal Research Fellow (Parasitology)

telephone: +44 (0) 141 330 4797
email: Lilach.Sheiner@glasgow.ac.uk

Research interests

Dr Sheiner is an expert in eukaryotic parasite cell biology and have been researching apicomplexan parasites (causing e.g. Toxoplasmosis and malaria) for over 15 years. 

Dr Sheiner's research team aims to understand basic mechanisms that underpin parasite survival and virulence, with focus on the role of their unique plastid (apicoplast) and mitochondrion. The team is mainly using T. gondii as a model organism, and recently commenced work with the human malaria parasite, Plasmodium falciparum

Lilach and her team developed some of the most widely used tools for Toxoplasma cell biology research1 and made substantial contribution to the understanding of plastids2,3,4 and mitochondria5,6 biology

Apicomplexan parasites are single-cell eukaryotes that evolved from free-living algae into global killers. Having acquired a mitochondrion, then a plastid, and then turning parasitic, these cells display a remarkable collection of globally conserved alongside parasite unique cellular structures and functions.

 

 

apicomplexan evolution

Our team asks how the plastid and mitochondrion are maintained (organelle protein import, sorting and folding, organelle transport) how do they communicate with each other and with other organelles (what is the role of membrane contact sites in parasite metabolism and homeostasis) and do these organelle’s functions contribute to parasite virulence and survival (what is their contribution to cellular redox regulation).

Selected publications

  1. Jacot D, Meissner M, Sheiner L, Soldati-Favre D and Striepen B. (2014) Genetic Manipulation of T. gondii. Toxoplasma Gondii (Second Edition). Chapter 17; Pages 577–611. (Editors:Kim k, and Weiss L).
  2. Sheiner L**, Demerly JL, Poulsen N, Beatty W, Lucas O, Behnke MS, White MW, and Striepen B.  A Systematic Screen to Discover and Analyze Apicoplast Proteins Identifies a Conserved and Essential Protein Import Factor. PLoS Pathogens. 2011 Dec;7(12):e1002392.
  3. Sheiner L**, Fellows JD, Ovciarikova J, Brooks CF, Agrawal S, Holmes ZC, Bietz I, Flinner N, Heiny S, Mirus O, Przyborski JM, Striepen B. Toxoplasma gondii Toc75 functions in import of stromal but not peripheral apicoplast proteins. Traffic. 2015 Sep 18. doi: 10.1111/tra.12333.
  4. Biddau M*, Bouchut A*, Major J*, Saveria T*, Tottey J*, Oka O, van-Lith M, Jennings KE, Ovciarikova J, DeRocher A, Striepen B, Waller RF, Parsons M and Sheiner L^ Two essential Thioredoxins mediate apicoplast biogenesis, protein import, and gene expression in Toxoplasma gondii. PLoS ppathogens. 2018 Feb 22;14(2):e1006836
  5. Jana Ovciarikova, Leandro Lemgruber, Krista L. Stilge, William J. Sulliva & Lilach Sheiner** Mitochondrial behaviour throughout the lytic cycle of Toxoplasma gondii Scientific Reports 2017, Feb 16;7:42746. doi: 10.1038/srep42746
  6. Sheiner L**, Vaidya AB, and McFadden GI. The metabolic roles of the endosymbiotic organelles of Toxoplasma and Plasmodium spp. Curr Opin Microbiol. 2013 Aug;16(4):452-8. doi: 10.1016/j.mib.2013.07.003

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Transgenic T. gondii as a platform for MeCP2 protein delivery to the CNS
    International Rett Syndrome Foundation
    2018 - 2020
     
  • P2D award
    Medical Research Council
    2018 - 2018
     
  • Dissection of the unusual mitochondrial tRNA import translocon of Toxoplasma gondii
    Biotechnology and Biological Sciences Research Council
    2016 - 2018
     
  • Dance with me? Mechanisms controlling mitochondrion - cell-cycle synchrony in Toxoplasma gondii
    The Royal Society of Edinburgh
    2015 - 2020
     
  • Identification and analysis of essential proteins involved in Toxoplasma gondii mitochondrial tRNA import (ISSF Fellowship)
    Wellcome Trust
    2015 - 2015
     
  • Identification and analysis of essential proteins involved in Toxoplasma gondii mitochondrial biogenesis (ISSF Catalyst)
    Wellcome Trust
    2014 - 2014
     

Additional information

Invited International Presentations

  • 2018: Rehovot, Israel - Weizmann Institute of Science. Frontiers in Parasitology
  • 2017: Caxambu, Brazil - Brazilian Protozoology Society Annual meeting. Invited Speaker and Panel Member for Round Table about Genome Editing in Parasitic Protozoa.
  • 2017: Glasgow, Scotland - University of Strathclyde. Invited seminar speaker.
  • 2017: Beer-Sheva, Israel - Ben Gurion University. Invited seminar speaker.
  • 2016: Montpellier, France - Université de Montpellier. Invited international speaker
  • 2016: Canterbury, UK - University of Kent, School of Biosciences seminar series. Invited seminar speaker.
  • 2016: Tuebingen, Germany - Max Planck Institute for Developmental Biology and Friedrich Miescher Laboratory International PhD Program annual retreat. Invited Keynote speaker.
  • 2016: Cambridge, UK - Cambridge Parasitology Club seminar series. Invited seminar speaker.
  • 2015: London, UK - The Francis Crick Institute (NIMR). Invited seminar speaker.
  • 2015: Edinburgh,UK - The University of Edinburgh, Institute of Immunology and Infection Seminar series. Invited seminar speaker.
  • 2015: Rio de Janeiro, Brazil - Fiocruz, Instituto Oswaldo Cruiz. Invited seminar speaker.
  • 2015: Curitiba, Brazil. - Fiocruz Institututo Carlos Chagas. Invited seminar.
  • 2015: Birmingham, UK - SGM Annual Conference 2015, “Mitochondria and related organelles in microbial eukaryotes” session. Invited speaker.
  • 2015: Ceske Budejovice, Czech Republic. - University of South Bohemia, Institute of parasitology. Invited seminar speaker.
  • 2013: Pennsylvania, USA - University of Pennsylvania, School of Veterinary Medicine. Invited lecture.
  • 2012: Jerusalem, Israel - Hebrew University of Jerusalem, Faculty of Medicine. Invited lecture.
  • 2012: Glasgow, UK - Glasgow Biomedical Research Centre, University of Glasgow. Invited lecture.
  • 2011: Tel Aviv, Israel - Tel-Aviv University, Faculty of Medicine. Invited lecture.

Media

  • 2018 - 2018: Independent - https://www.independent.co.uk/life-style/health-and-families/health-news/cat-parasite-treatment-malaria-toxoplasma-gondii-birth-defects-miscarriage-a8228731.html
  • 2018 - 2018: The Scottish Farmer - http://www.thescottishfarmer.co.uk/news/16043972.Toxoplasmosis_disease_breakthrough/
  • 2018 - 2018: The Conversation - https://theconversation.com/finding-the-achilles-heel-of-the-cat-parasite-could-mean-more-effective-treatment-for-toxoplasmosis-and-malaria-92232

Prizes, Awards and Distinctions

  • 2016: co-Founder and co-Director, Middle East Biology of Parasitism http://www.middle-eastbop.com/
  • 2015: Royal Society of Edinburgh Personal Research Fellowship
  • 2013: Athens, Georgia, USA - NIH pathways to independence

Research Fellowship

  • 2013 - 2013: Glasgow, Scotland - Lord Kelvin Adam Smith (LKAS) Leadership Fellowship
  • 2010 - 2010: Athens, Georgia, USA - Swiss National Fund "recherche debutant" fellowship
  • 2009 - 2009: Athens, Georgia, USA - Swiss National Fund "recherche debutant" fellowship
  • 2008 - 2008: Geneva, Switzerland - MBL Pioneer Scholarship Fund and the Herbert W. Rand Fellowship and Scholarship Fund
  • 2003 - 2003: Bern, Switzerland - EMBO Short Fellowship 2003

Publications

List by: Type | Date

Jump to: 2018 | 2017 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2008 | 2007
Number of items: 22.

2018

Biddau, M. et al. (2018) Two essential Thioredoxins mediate apicoplast biogenesis, protein import, and gene expression in Toxoplasma gondii. PLoS Pathogens, 14(2), e1006836. (doi:10.1371/journal.ppat.1006836) (PMID:29470517) (PMCID:PMC5823475)

2017

Ovciarikova, J., Lemgruber, L., Stilger, K. L., Sullivan, W. J. and Sheiner, L. (2017) Mitochondrial behaviour throughout the lytic cycle of Toxoplasma gondii. Scientific Reports, 7, 42746. (doi:10.1038/srep42746) (PMID:28202940) (PMCID:PMC5311943)

2015

Sheiner, L. et al. (2015) Toxoplasma gondii Toc75 functions in import of stromal but not peripheral apicoplast proteins. Traffic, 16(12), pp. 1254-1269. (doi:10.1111/tra.12333) (PMID:26381927)

Williams, M. J., Alonso, H., Enciso, M., Egarter, S., Sheiner, L., Meissner, M., Striepen, B., Smith, B. J. and Tonkin, C. J. (2015) Two essential light chains regulate the MyoA lever arm to promote Toxoplasma gliding motility. mBio, 6(5), e00845-15. (doi:10.1128/mBio.00845-15) (PMID:26374117) (PMCID:PMC4600101)

2014

Daher, W., Morlon-Guyot, J., Sheiner, L., Lentini, G., Berry, L., Tawk, L., Dubremetz, J.-F., Wengelnik, K., Striepen, B. and Lebrun, M. (2014) Lipid kinases are essential for apicoplast homeostasis inToxoplasma gondii. Cellular Microbiology, 17(4), pp. 559-578. (doi:10.1111/cmi.12383)

Jacot, D., Meissner, M., Sheiner, L., Soldati-Favre, D. and Striepen, B. (2014) Genetic manipulation of toxoplasma gondii. In: Weiss, L.M. and Kim, K. (eds.) Toxoplasma Gondii. Elsevier Academic Press: Burlington, VT, pp. 577-611. ISBN 9780123965363 (doi:10.1016/B978-0-12-396481-6.00017-9)

Sheiner, L. and Striepen, B. (2014) The apicoplast: a parasite’s symbiont. In: Theg, S. M. and Wollman, F.-A. (eds.) Plastid Biology. Series: Advances in plant biology (5). Springer New York: New York, pp. 209-238. ISBN 9781493911356 (doi:10.1007/978-1-4939-1136-3_8)

2013

Sheiner, L., Vaidya, A.B. and McFadden, G.I. (2013) The metabolic roles of the endosymbiotic organelles of Toxoplasma and Plasmodium spp. Current Opinion in Microbiology, 16(4), pp. 452-458. (doi:10.1016/j.mib.2013.07.003)

Sheiner, L. and Striepen, B. (2013) Protein sorting in complex plastids. Biochimica et Biophysica Acta: Molecular Cell Research, 1833(2), pp. 352-359. (doi:10.1016/j.bbamcr.2012.05.030)

2012

Francia, M.E., Jordan, C.N., Patel, J.D., Sheiner, L., Demerly, J.L., Fellows, J.D., de Leon, J.C., Morrissette, N.S., Dubremetz, J.-F. and Striepen, B. (2012) Cell division in apicomplexan parasites is organized by a homolog of the striated rootlet fiber of algal flagella. PLoS Biology, 10(12), e1001444. (doi:10.1371/journal.pbio.1001444) (PMID:23239939)

MacRae, J.I., Sheiner, L., Nahid, A., Tonkin, C., Striepen, B. and McConville, M.J. (2012) Mitochondrial metabolism of glucose and glutamine is required for intracellular growth of Toxoplasma gondii. Cell Host and Microbe, 12(5), pp. 682-692. (doi:10.1016/j.chom.2012.09.013)

Sampels, V., Hartmann, A., Dietrich, I., Coppens, I., Sheiner, L., Striepen, B., Herrmann, A., Lucius, R. and Gupta, N. (2012) Mutagenesis of a novel choline kinase demonstrates plasticity of phosphatidylcholine biogenesis and gene expression in Toxoplasma gondii. Journal of Biological Chemistry, 287(20), pp. 16289-16299. (doi:10.1074/jbc.M112.347138)

2011

Sheiner, L., Demerly, J.L., Poulsen, N., Beatty, W.L., Lucas, O., Behnke, M.S., White, M.W. and Striepen, B. (2011) A systematic screen to discover and analyze apicoplast proteins identifies a conserved and essential protein import factor. PLoS Pathogens, 7(12), e1002392. (doi:10.1371/journal.ppat.1002392)

2010

Agrawal, S., Nair, S., Sheiner, L. and Striepen, B. (2010) The Apicoplast: An ancient algal endosymbiont of Apicomplexa. In: de Souza, W. (ed.) Structures and Organelles in Pathogenic Protists. Series: Microbiology monographs (17). Springer. ISBN 9783642128639

Lorestani, A., Sheiner, L., Yang, K., Robertson, S.D., Sahoo, N., Brooks, C.F., Ferguson, D.J.P., Striepen, B. and Gubbels, M.-J. (2010) A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis. PLoS ONE, 5(8), e12302. (doi:10.1371/journal.pone.0012302)

Pino, P., Aeby, E., Foth, B.J., Sheiner, L., Soldati, T., Schneider, A. and Soldati-Favre, D. (2010) Mitochondrial translation in absence of local tRNA aminoacylation and methionyl tRNAMet formylation in Apicomplexa. Molecular Microbiology, 76(3), pp. 706-718. (doi:10.1111/j.1365-2958.2010.07128.x)

Sheiner, L., Santos, J.M., Klages, N., Parussini, F., Jemmely, N., Friedrich, N., Ward, G.E. and Soldati-Favre, D. (2010) Toxoplasma gondii transmembrane microneme proteins and their modular design. Molecular Microbiology, 77(4), pp. 912-929. (doi:10.1111/j.1365-2958.2010.07255.x)

2008

Sheiner, L., Dowse, T.J. and Soldati-Favre, D. (2008) Identification of trafficking determinants for polytopic rhomboid proteases in toxoplasma gondii. Traffic, 9(5), pp. 665-677. (doi:10.1111/j.1600-0854.2008.00736.x)

Goldshmidt, H., Sheiner, L., Butikofer, P., Roditi, I., Uliel, S., Gunzel, M., Engstler, M. and Michaeli, S. (2008) Role of protein translocation pathways across the endoplasmic reticulum in Trypanosoma brucei. Journal of Biological Chemistry, 283(46), pp. 32085-32098. (doi:10.1074/jbc.M801499200)

Sheiner, L. and Soldati-Favre, D. (2008) Protein trafficking inside Toxoplasma gondii. Traffic, 9(5), pp. 636-646. (doi:10.1111/j.1600-0854.2008.00713.x)

2007

Lustig, Y., Sheiner, L., Vagima, Y., Goldshmidt, H., Das, A., Bellofatto, V. and Michaeli, S. (2007) Spliced-leader RNA silencing: a novel stress-induced mechanism in Trypanosoma brucei. EMBO Reports, 8(4), pp. 408-413. (doi:10.1038/sj.embor.7400930)

Pino, P., Foth, B.J., Kwok, L.-Y., Sheiner, L., Schepers, R., Soldati, T. and Soldati-Favre, D. (2007) Dual targeting of antioxidant and metabolic enzymes to the mitochondrion and the apicoplast of Toxoplasma gondii. PLoS Pathogens, 3(8), e115. (doi:10.1371/journal.ppat.0030115)

This list was generated on Sat Jul 21 14:15:39 2018 BST.
Number of items: 22.

Articles

Biddau, M. et al. (2018) Two essential Thioredoxins mediate apicoplast biogenesis, protein import, and gene expression in Toxoplasma gondii. PLoS Pathogens, 14(2), e1006836. (doi:10.1371/journal.ppat.1006836) (PMID:29470517) (PMCID:PMC5823475)

Ovciarikova, J., Lemgruber, L., Stilger, K. L., Sullivan, W. J. and Sheiner, L. (2017) Mitochondrial behaviour throughout the lytic cycle of Toxoplasma gondii. Scientific Reports, 7, 42746. (doi:10.1038/srep42746) (PMID:28202940) (PMCID:PMC5311943)

Sheiner, L. et al. (2015) Toxoplasma gondii Toc75 functions in import of stromal but not peripheral apicoplast proteins. Traffic, 16(12), pp. 1254-1269. (doi:10.1111/tra.12333) (PMID:26381927)

Williams, M. J., Alonso, H., Enciso, M., Egarter, S., Sheiner, L., Meissner, M., Striepen, B., Smith, B. J. and Tonkin, C. J. (2015) Two essential light chains regulate the MyoA lever arm to promote Toxoplasma gliding motility. mBio, 6(5), e00845-15. (doi:10.1128/mBio.00845-15) (PMID:26374117) (PMCID:PMC4600101)

Daher, W., Morlon-Guyot, J., Sheiner, L., Lentini, G., Berry, L., Tawk, L., Dubremetz, J.-F., Wengelnik, K., Striepen, B. and Lebrun, M. (2014) Lipid kinases are essential for apicoplast homeostasis inToxoplasma gondii. Cellular Microbiology, 17(4), pp. 559-578. (doi:10.1111/cmi.12383)

Sheiner, L., Vaidya, A.B. and McFadden, G.I. (2013) The metabolic roles of the endosymbiotic organelles of Toxoplasma and Plasmodium spp. Current Opinion in Microbiology, 16(4), pp. 452-458. (doi:10.1016/j.mib.2013.07.003)

Sheiner, L. and Striepen, B. (2013) Protein sorting in complex plastids. Biochimica et Biophysica Acta: Molecular Cell Research, 1833(2), pp. 352-359. (doi:10.1016/j.bbamcr.2012.05.030)

Francia, M.E., Jordan, C.N., Patel, J.D., Sheiner, L., Demerly, J.L., Fellows, J.D., de Leon, J.C., Morrissette, N.S., Dubremetz, J.-F. and Striepen, B. (2012) Cell division in apicomplexan parasites is organized by a homolog of the striated rootlet fiber of algal flagella. PLoS Biology, 10(12), e1001444. (doi:10.1371/journal.pbio.1001444) (PMID:23239939)

MacRae, J.I., Sheiner, L., Nahid, A., Tonkin, C., Striepen, B. and McConville, M.J. (2012) Mitochondrial metabolism of glucose and glutamine is required for intracellular growth of Toxoplasma gondii. Cell Host and Microbe, 12(5), pp. 682-692. (doi:10.1016/j.chom.2012.09.013)

Sampels, V., Hartmann, A., Dietrich, I., Coppens, I., Sheiner, L., Striepen, B., Herrmann, A., Lucius, R. and Gupta, N. (2012) Mutagenesis of a novel choline kinase demonstrates plasticity of phosphatidylcholine biogenesis and gene expression in Toxoplasma gondii. Journal of Biological Chemistry, 287(20), pp. 16289-16299. (doi:10.1074/jbc.M112.347138)

Sheiner, L., Demerly, J.L., Poulsen, N., Beatty, W.L., Lucas, O., Behnke, M.S., White, M.W. and Striepen, B. (2011) A systematic screen to discover and analyze apicoplast proteins identifies a conserved and essential protein import factor. PLoS Pathogens, 7(12), e1002392. (doi:10.1371/journal.ppat.1002392)

Lorestani, A., Sheiner, L., Yang, K., Robertson, S.D., Sahoo, N., Brooks, C.F., Ferguson, D.J.P., Striepen, B. and Gubbels, M.-J. (2010) A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis. PLoS ONE, 5(8), e12302. (doi:10.1371/journal.pone.0012302)

Pino, P., Aeby, E., Foth, B.J., Sheiner, L., Soldati, T., Schneider, A. and Soldati-Favre, D. (2010) Mitochondrial translation in absence of local tRNA aminoacylation and methionyl tRNAMet formylation in Apicomplexa. Molecular Microbiology, 76(3), pp. 706-718. (doi:10.1111/j.1365-2958.2010.07128.x)

Sheiner, L., Santos, J.M., Klages, N., Parussini, F., Jemmely, N., Friedrich, N., Ward, G.E. and Soldati-Favre, D. (2010) Toxoplasma gondii transmembrane microneme proteins and their modular design. Molecular Microbiology, 77(4), pp. 912-929. (doi:10.1111/j.1365-2958.2010.07255.x)

Sheiner, L., Dowse, T.J. and Soldati-Favre, D. (2008) Identification of trafficking determinants for polytopic rhomboid proteases in toxoplasma gondii. Traffic, 9(5), pp. 665-677. (doi:10.1111/j.1600-0854.2008.00736.x)

Goldshmidt, H., Sheiner, L., Butikofer, P., Roditi, I., Uliel, S., Gunzel, M., Engstler, M. and Michaeli, S. (2008) Role of protein translocation pathways across the endoplasmic reticulum in Trypanosoma brucei. Journal of Biological Chemistry, 283(46), pp. 32085-32098. (doi:10.1074/jbc.M801499200)

Sheiner, L. and Soldati-Favre, D. (2008) Protein trafficking inside Toxoplasma gondii. Traffic, 9(5), pp. 636-646. (doi:10.1111/j.1600-0854.2008.00713.x)

Lustig, Y., Sheiner, L., Vagima, Y., Goldshmidt, H., Das, A., Bellofatto, V. and Michaeli, S. (2007) Spliced-leader RNA silencing: a novel stress-induced mechanism in Trypanosoma brucei. EMBO Reports, 8(4), pp. 408-413. (doi:10.1038/sj.embor.7400930)

Pino, P., Foth, B.J., Kwok, L.-Y., Sheiner, L., Schepers, R., Soldati, T. and Soldati-Favre, D. (2007) Dual targeting of antioxidant and metabolic enzymes to the mitochondrion and the apicoplast of Toxoplasma gondii. PLoS Pathogens, 3(8), e115. (doi:10.1371/journal.ppat.0030115)

Book Sections

Jacot, D., Meissner, M., Sheiner, L., Soldati-Favre, D. and Striepen, B. (2014) Genetic manipulation of toxoplasma gondii. In: Weiss, L.M. and Kim, K. (eds.) Toxoplasma Gondii. Elsevier Academic Press: Burlington, VT, pp. 577-611. ISBN 9780123965363 (doi:10.1016/B978-0-12-396481-6.00017-9)

Sheiner, L. and Striepen, B. (2014) The apicoplast: a parasite’s symbiont. In: Theg, S. M. and Wollman, F.-A. (eds.) Plastid Biology. Series: Advances in plant biology (5). Springer New York: New York, pp. 209-238. ISBN 9781493911356 (doi:10.1007/978-1-4939-1136-3_8)

Agrawal, S., Nair, S., Sheiner, L. and Striepen, B. (2010) The Apicoplast: An ancient algal endosymbiont of Apicomplexa. In: de Souza, W. (ed.) Structures and Organelles in Pathogenic Protists. Series: Microbiology monographs (17). Springer. ISBN 9783642128639

This list was generated on Sat Jul 21 14:15:39 2018 BST.