First publication from SCMI

Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA

Dr Martin Rennie from Prof Helen Walden's group at the Institute of Molecular, Cell and Systems Biology together with others including the SCMI, have demonstrated a function for the ubiquitination of two subunits (FANCI and FANCD2) of the ID2 complex in the Fanconi Anaemia pathway, a pathway which is dedicated to DNA repair.

They show that ubiquitination of FANCD2 increases affinity of the complex for double-stranded DNA, whereas ubiquitination of FANCI protects FANCD2 from attack by deubiquitinases.

In the paper, they used cryo-EM to solve the structure of two complexes; ID2Ub and IUbD2Ub‐dsDNA. Grids for both complexes were prepared and screened using the SCMI's F2-Cryo electron microscope. The published structure of ID2Ub was then solved using the SCMI's JEOL CRYO ARM 300, with the IUbD2Ub‐dsDNA being solved at eBIC.

The full paper in EMBO Reports can be accessed here.


First published: 11 June 2020

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