Mrs Julia Dunlop

  • Research Technician (Institute of Molecular Cell & Systems Biology Administration)

Biography

Key Skills 

Core Funded

I am currently lab manager for Prof Kostas Tokatlidis. The lab management responsibilities I have are for;

  • Health and Safety, generation and review of COSHH forms.
  • Adherence to regulations for working with radioisotopes.
  • GM risk assessment.
  • Involvement in training of undergraduate and post-graduate students.
  • Ordering, keeping track of expenditure and budgeting.
  • Sourcing, purchase and maintenance of lab equipment.
  • Keeping the lab clean, tidy, usable and safe.

My IT skills are competency in Office, Word, Excel for communication and data management and I have recent experience of data analysis in Prism.

I have 30 years experience of laboratory bench work but currently I am proficient in:

  • Cell culture; maintenance of cell lines and setting up and performing cell based assays.
  • Molecular biology techniques including PCR, working with RNA and DNA, gel electrophoresis, sub-cloning and site-directed mutagenesis.
  • Protein purification, SDS-PAGE and Western blotting.
  • Preparation of Mitochondria from yeast cell cultures and subsequent mitochondrial import assays.
  • 35S GTPyS assays and BRET assays for screening of pharmacological compounds.

 


Research interests

I am currently working in the lab of Prof Kostas Tokatlidis focusing on Mitochondrial Biogenesis in Health and Disease. Over my career I have worked on vaccine development for bacterial disease in farmed Atlantic Salmon, Herpes Simplex Virus, Human Papilloma Virus, Cancer Research, Inflammatory Pathway Biochemistry, and Molecular Pharmacology.


Publications

List by: Type | Date

Jump to: 2017 | 2016 | 2014 | 2013 | 2012
Number of items: 9.

2017

Kaspersen, M. H., Jenkins, L. , Dunlop, J. , Milligan, G. and Ulven, T. (2017) Succinct synthesis of saturated hydroxy fatty acids and in vitro evaluation of all hydroxylauric acids on FFA1, FFA4 and GPR84. MedChemComm, 8(6), pp. 1360-1365. (doi: 10.1039/c7md00130d)

Watterson, K. R. et al. (2017) Probe-dependent negative allosteric modulators of the long-chain free fatty acid receptor FFA4. Molecular Pharmacology, 91(6), pp. 630-641. (doi: 10.1124/mol.116.107821) (PMID:28385906) (PMCID:PMC5438128)

Chen, S.C., Brooks, R., Houskeeper, J., Bremner, S.K., Dunlop, J. , Viollet, B., Logan, P.J., Salt, I.P. , Ahmed, S.F. and Yarwood, S.J. (2017) Corrigendum to "Metformin suppresses adipogenesis through both AMP-activated protein kinase (AMPK)-dependent and AMPK-independent mechanisms" [Mol. Cell. Endocrinol. 440 15 January 2017 57-68]. Molecular and Cellular Endocrinology, 443, p. 176. (doi: 10.1016/j.mce.2017.01.049) (PMID:28183460)

Chen, S. C., Brooks, R., Houskeeper, J., Bremner, S. K., Dunlop, J. , Viollet, B., Logan, P. J., Salt, I. P. , Ahmed, S. F. and Yarwood, S. J. (2017) Metformin suppresses adipogenesis through both AMP-activated protein kinase (AMPK)-dependent and AMPK-independent mechanisms. Molecular and Cellular Endocrinology, 440, pp. 57-68. (doi: 10.1016/j.mce.2016.11.011) (PMID:27856330) (PMCID:PMC5228588)

2016

Azevedo, C. M. G. et al. (2016) Non-acidic free fatty acid receptor 4 agonists with antidiabetic activity. Journal of Medicinal Chemistry, 59(19), pp. 8868-8878. (doi: 10.1021/acs.jmedchem.6b00685)

2014

Wiejak, J., Dunlop, J. and Yarwood, S. J. (2014) The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs. FEBS Letters, 588(9), pp. 1556-1561. (doi: 10.1016/j.febslet.2014.02.038)

2013

Wiejak, J., Dunlop, J. , Mackay, S. P. and Yarwood, S. J. (2013) Flavanoids induce expression of the suppressor of cytokine signalling 3 (SOCS3) gene and suppress IL6- activated signal transducer and activator of transcription 3 (STAT3) activation in vascular endothelial cells. Biochemical Journal, 454(2), pp. 283-293. (doi: 10.1042/BJ20130481)

2012

Wiejak, J., Dunlop, J. , Stoyle, C., Lappin, G., McIlroy, A., Pediani, J. D. , Gao, S. and Yarwood, S. J. (2012) The protein kinase C inhibitor, Ro-31-7459, is a potent activator of ERK and JNK MAP kinases in HUVECs and yet inhibits cyclic AMP-stimulated SOCS-3 gene induction through inactivation of the transcription factor c-Jun. Cellular Signalling, 24(8), pp. 1690-1699. (doi: 10.1016/j.cellsig.2012.04.016)

Wiejak, J., Dunlop, J. , Gao, S., Borland, G. and Yarwood, S. J. (2012) Extracellular signal-regulated kinase mitogen-activated protein kinase-dependent SOCS-3 gene induction requires c-Jun, signal transducer and activator of transcription 3, and specificity protein 3 transcription factors. Molecular Pharmacology, 81(5), pp. 657-668. (doi: 10.1124/mol.111.076976)

This list was generated on Wed Jul 8 14:38:25 2020 BST.
Jump to: Articles
Number of items: 9.

Articles

Kaspersen, M. H., Jenkins, L. , Dunlop, J. , Milligan, G. and Ulven, T. (2017) Succinct synthesis of saturated hydroxy fatty acids and in vitro evaluation of all hydroxylauric acids on FFA1, FFA4 and GPR84. MedChemComm, 8(6), pp. 1360-1365. (doi: 10.1039/c7md00130d)

Watterson, K. R. et al. (2017) Probe-dependent negative allosteric modulators of the long-chain free fatty acid receptor FFA4. Molecular Pharmacology, 91(6), pp. 630-641. (doi: 10.1124/mol.116.107821) (PMID:28385906) (PMCID:PMC5438128)

Chen, S.C., Brooks, R., Houskeeper, J., Bremner, S.K., Dunlop, J. , Viollet, B., Logan, P.J., Salt, I.P. , Ahmed, S.F. and Yarwood, S.J. (2017) Corrigendum to "Metformin suppresses adipogenesis through both AMP-activated protein kinase (AMPK)-dependent and AMPK-independent mechanisms" [Mol. Cell. Endocrinol. 440 15 January 2017 57-68]. Molecular and Cellular Endocrinology, 443, p. 176. (doi: 10.1016/j.mce.2017.01.049) (PMID:28183460)

Chen, S. C., Brooks, R., Houskeeper, J., Bremner, S. K., Dunlop, J. , Viollet, B., Logan, P. J., Salt, I. P. , Ahmed, S. F. and Yarwood, S. J. (2017) Metformin suppresses adipogenesis through both AMP-activated protein kinase (AMPK)-dependent and AMPK-independent mechanisms. Molecular and Cellular Endocrinology, 440, pp. 57-68. (doi: 10.1016/j.mce.2016.11.011) (PMID:27856330) (PMCID:PMC5228588)

Azevedo, C. M. G. et al. (2016) Non-acidic free fatty acid receptor 4 agonists with antidiabetic activity. Journal of Medicinal Chemistry, 59(19), pp. 8868-8878. (doi: 10.1021/acs.jmedchem.6b00685)

Wiejak, J., Dunlop, J. and Yarwood, S. J. (2014) The role of c-Jun in controlling the EPAC1-dependent induction of the SOCS3 gene in HUVECs. FEBS Letters, 588(9), pp. 1556-1561. (doi: 10.1016/j.febslet.2014.02.038)

Wiejak, J., Dunlop, J. , Mackay, S. P. and Yarwood, S. J. (2013) Flavanoids induce expression of the suppressor of cytokine signalling 3 (SOCS3) gene and suppress IL6- activated signal transducer and activator of transcription 3 (STAT3) activation in vascular endothelial cells. Biochemical Journal, 454(2), pp. 283-293. (doi: 10.1042/BJ20130481)

Wiejak, J., Dunlop, J. , Stoyle, C., Lappin, G., McIlroy, A., Pediani, J. D. , Gao, S. and Yarwood, S. J. (2012) The protein kinase C inhibitor, Ro-31-7459, is a potent activator of ERK and JNK MAP kinases in HUVECs and yet inhibits cyclic AMP-stimulated SOCS-3 gene induction through inactivation of the transcription factor c-Jun. Cellular Signalling, 24(8), pp. 1690-1699. (doi: 10.1016/j.cellsig.2012.04.016)

Wiejak, J., Dunlop, J. , Gao, S., Borland, G. and Yarwood, S. J. (2012) Extracellular signal-regulated kinase mitogen-activated protein kinase-dependent SOCS-3 gene induction requires c-Jun, signal transducer and activator of transcription 3, and specificity protein 3 transcription factors. Molecular Pharmacology, 81(5), pp. 657-668. (doi: 10.1124/mol.111.076976)

This list was generated on Wed Jul 8 14:38:25 2020 BST.