Dr Karen McLuskey
- Research Associate (Molecular Biosciences)
Publications
2024
Myburgh, Elmarie, Geoghegan, Vincent, Alves-Ferreira, Eliza V.C., Nievas, Y. Romina, Grewal, Jaspreet S., Brown, Elaine, McLuskey, Karen and Mottram, Jeremy C. (2024) TORC1 is an essential regulator of nutrient-controlled proliferation and differentiation in Leishmania. EMBO Reports, 25(3), pp. 1075-1105. (doi: 10.1038/s44319-024-00084-y) (PMID:38396206) (PMCID:PMC10933368)
2021
McLuskey, Karen, Wandy, Joe ORCID: https://orcid.org/0000-0002-3068-4664, Vincent, Isabel
ORCID: https://orcid.org/0000-0003-2663-4389, Van Der Hooft, Justin J.J.
ORCID: https://orcid.org/0000-0002-9340-5511, Rogers, Simon
ORCID: https://orcid.org/0000-0003-3578-4477, Burgess, Karl and Daly, Ronan
ORCID: https://orcid.org/0000-0002-1275-6820
(2021)
Ranking metabolite sets by their activity levels.
Metabolites, 11(2),
103.
(doi: 10.3390/metabo11020103)
(PMID:33670102)
(PMCID:PMC7916825)
2016
Grewal, J. S. et al. (2016) PNT1 is a C11 cysteine peptidase essential for replication of the trypanosome kinetoplast. Journal of Biological Chemistry, 291, pp. 9492-9500. (doi: 10.1074/jbc.M116.714972) (PMID:26940875) (PMCID:PMC4850289)
McLuskey, Karen, Grewal, Jaspreet S., Das, Debanu, Godzik, Adam, Lesley, Scott A., Deacon, Ashley M., Coombs, Graham H., Elsliger, Marc-André, Wilson, Ian A. and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2016)
Crystal structure and activity studies of the C11 cysteine peptidase from Parabacteroides merdae in the human gut microbiome.
Journal of Biological Chemistry, 291,
pp. 9482-9491.
(doi: 10.1074/jbc.M115.706143)
(PMID:26940874)
(PMCID:PMC4850288)
2015
McLuskey, Karen and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2015)
Comparative structural analysis of the caspase family with other clan CD cysteine peptidases.
Biochemical Journal, 466(2),
pp. 219-232.
(doi: 10.1042/BJ20141324)
(PMID:25697094)
(PMCID:PMC4357240)
2014
McLuskey, Karen, Moss, Catherine X. and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2014)
Purification, characterization, and crystallization of trypanosoma metacaspases.
Methods in Molecular Biology, 1133,
pp. 203-221.
(doi: 10.1007/978-1-4939-0357-3_13)
2013
Machado, M.K.M, Marcondes, M.F., Juliano, M.A., McLuskey, K., Mottram, J.C. ORCID: https://orcid.org/0000-0001-5574-3766, Moss, C.X., Juliano, L. and Oliveira, V.
(2013)
Substrate specificity and the effect of calcium on Trypanosomabrucei metacaspase 2.
FEBS Journal, 280(11),
pp. 2608-2621.
(doi: 10.1111/febs.12248)
Gabrielsen, M. ORCID: https://orcid.org/0000-0002-9848-2276, Roe, A.J.
ORCID: https://orcid.org/0000-0003-3698-6134 and McLuskey, K.
(2013)
High-throughput methods for the detection of protein overexpression using fluorescence markers.
Methods in Cell Biology, 113,
pp. 189-208.
(doi: 10.1016/B978-0-12-407239-8.00010-0)
2012
McLuskey, Karen, Rudolf, Jana, Proto, William R., Isaacs, Neil W., Coombs, Graham H., Moss, Catherine X. and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2012)
Crystal structure of a Trypanosoma brucei metacaspase.
Proceedings of the National Academy of Sciences of the United States of America, 109(19),
pp. 7469-7474.
(doi: 10.1073/pnas.1200885109)
2011
Gabrielsen, M. ORCID: https://orcid.org/0000-0002-9848-2276, Kroner, F., Black, I., Isaacs, N.W., Roe, A.J.
ORCID: https://orcid.org/0000-0003-3698-6134 and McLuskey, K.
(2011)
High-throughput identification of purification conditions leads to preliminary crystallization conditions for three inner membrane proteins.
Molecular Membrane Biology, 28(7-8),
pp. 445-453.
(doi: 10.3109/09687688.2011.628954)
(PMID:22034843)
Munday, Jane C. ORCID: https://orcid.org/0000-0001-7792-2749, McLuskey, Karen, Brown, Elaine, Coombs, Graham H. and Mottram, Jeremy C.
ORCID: https://orcid.org/0000-0001-5574-3766
(2011)
Oligopeptidase B deficient mutants of Leishmania major.
Molecular and Biochemical Parasitology, 175(1),
pp. 49-57.
(doi: 10.1016/j.molbiopara.2010.09.003)
2010
McLuskey, Karen, Paterson, Neil G., Bland, Nicholas D., Isaacs, Neil W. and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2010)
Crystal structure of leishmania major oligopeptidase B gives insight into the enzymatic properties of a trypanosomatid virulence factor.
Journal of Biological Chemistry, 285(50),
pp. 39249-39259.
(doi: 10.1074/jbc.M110.156679)
McLuskey, K., Roszak, A. W., Zhu, Y. S. and Isaacs, N. W. (2010) Crystal structures of all-alpha type membrane proteins. European Biophysics Journal with Biophysics Letters, 39(5), pp. 723-755. (doi: 10.1007/s00249-009-0546-6)
2008
McLuskey, K., Gabrielsen, M. ORCID: https://orcid.org/0000-0002-9848-2276, Kroner, Frank, Black, I., Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Isaacs, N.W.
(2008)
A protocol for high throughput methods for the expression and purification of inner membrane proteins.
Molecular Membrane Biology, 25(8),
pp. 599-608.
(doi: 10.1080/09687680802510289)
Articles
Myburgh, Elmarie, Geoghegan, Vincent, Alves-Ferreira, Eliza V.C., Nievas, Y. Romina, Grewal, Jaspreet S., Brown, Elaine, McLuskey, Karen and Mottram, Jeremy C. (2024) TORC1 is an essential regulator of nutrient-controlled proliferation and differentiation in Leishmania. EMBO Reports, 25(3), pp. 1075-1105. (doi: 10.1038/s44319-024-00084-y) (PMID:38396206) (PMCID:PMC10933368)
McLuskey, Karen, Wandy, Joe ORCID: https://orcid.org/0000-0002-3068-4664, Vincent, Isabel
ORCID: https://orcid.org/0000-0003-2663-4389, Van Der Hooft, Justin J.J.
ORCID: https://orcid.org/0000-0002-9340-5511, Rogers, Simon
ORCID: https://orcid.org/0000-0003-3578-4477, Burgess, Karl and Daly, Ronan
ORCID: https://orcid.org/0000-0002-1275-6820
(2021)
Ranking metabolite sets by their activity levels.
Metabolites, 11(2),
103.
(doi: 10.3390/metabo11020103)
(PMID:33670102)
(PMCID:PMC7916825)
Grewal, J. S. et al. (2016) PNT1 is a C11 cysteine peptidase essential for replication of the trypanosome kinetoplast. Journal of Biological Chemistry, 291, pp. 9492-9500. (doi: 10.1074/jbc.M116.714972) (PMID:26940875) (PMCID:PMC4850289)
McLuskey, Karen, Grewal, Jaspreet S., Das, Debanu, Godzik, Adam, Lesley, Scott A., Deacon, Ashley M., Coombs, Graham H., Elsliger, Marc-André, Wilson, Ian A. and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2016)
Crystal structure and activity studies of the C11 cysteine peptidase from Parabacteroides merdae in the human gut microbiome.
Journal of Biological Chemistry, 291,
pp. 9482-9491.
(doi: 10.1074/jbc.M115.706143)
(PMID:26940874)
(PMCID:PMC4850288)
McLuskey, Karen and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2015)
Comparative structural analysis of the caspase family with other clan CD cysteine peptidases.
Biochemical Journal, 466(2),
pp. 219-232.
(doi: 10.1042/BJ20141324)
(PMID:25697094)
(PMCID:PMC4357240)
McLuskey, Karen, Moss, Catherine X. and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2014)
Purification, characterization, and crystallization of trypanosoma metacaspases.
Methods in Molecular Biology, 1133,
pp. 203-221.
(doi: 10.1007/978-1-4939-0357-3_13)
Machado, M.K.M, Marcondes, M.F., Juliano, M.A., McLuskey, K., Mottram, J.C. ORCID: https://orcid.org/0000-0001-5574-3766, Moss, C.X., Juliano, L. and Oliveira, V.
(2013)
Substrate specificity and the effect of calcium on Trypanosomabrucei metacaspase 2.
FEBS Journal, 280(11),
pp. 2608-2621.
(doi: 10.1111/febs.12248)
Gabrielsen, M. ORCID: https://orcid.org/0000-0002-9848-2276, Roe, A.J.
ORCID: https://orcid.org/0000-0003-3698-6134 and McLuskey, K.
(2013)
High-throughput methods for the detection of protein overexpression using fluorescence markers.
Methods in Cell Biology, 113,
pp. 189-208.
(doi: 10.1016/B978-0-12-407239-8.00010-0)
McLuskey, Karen, Rudolf, Jana, Proto, William R., Isaacs, Neil W., Coombs, Graham H., Moss, Catherine X. and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2012)
Crystal structure of a Trypanosoma brucei metacaspase.
Proceedings of the National Academy of Sciences of the United States of America, 109(19),
pp. 7469-7474.
(doi: 10.1073/pnas.1200885109)
Gabrielsen, M. ORCID: https://orcid.org/0000-0002-9848-2276, Kroner, F., Black, I., Isaacs, N.W., Roe, A.J.
ORCID: https://orcid.org/0000-0003-3698-6134 and McLuskey, K.
(2011)
High-throughput identification of purification conditions leads to preliminary crystallization conditions for three inner membrane proteins.
Molecular Membrane Biology, 28(7-8),
pp. 445-453.
(doi: 10.3109/09687688.2011.628954)
(PMID:22034843)
Munday, Jane C. ORCID: https://orcid.org/0000-0001-7792-2749, McLuskey, Karen, Brown, Elaine, Coombs, Graham H. and Mottram, Jeremy C.
ORCID: https://orcid.org/0000-0001-5574-3766
(2011)
Oligopeptidase B deficient mutants of Leishmania major.
Molecular and Biochemical Parasitology, 175(1),
pp. 49-57.
(doi: 10.1016/j.molbiopara.2010.09.003)
McLuskey, Karen, Paterson, Neil G., Bland, Nicholas D., Isaacs, Neil W. and Mottram, Jeremy C. ORCID: https://orcid.org/0000-0001-5574-3766
(2010)
Crystal structure of leishmania major oligopeptidase B gives insight into the enzymatic properties of a trypanosomatid virulence factor.
Journal of Biological Chemistry, 285(50),
pp. 39249-39259.
(doi: 10.1074/jbc.M110.156679)
McLuskey, K., Roszak, A. W., Zhu, Y. S. and Isaacs, N. W. (2010) Crystal structures of all-alpha type membrane proteins. European Biophysics Journal with Biophysics Letters, 39(5), pp. 723-755. (doi: 10.1007/s00249-009-0546-6)
McLuskey, K., Gabrielsen, M. ORCID: https://orcid.org/0000-0002-9848-2276, Kroner, Frank, Black, I., Cogdell, R.J.
ORCID: https://orcid.org/0000-0003-2119-6607 and Isaacs, N.W.
(2008)
A protocol for high throughput methods for the expression and purification of inner membrane proteins.
Molecular Membrane Biology, 25(8),
pp. 599-608.
(doi: 10.1080/09687680802510289)
Grants
Grants and Awards listed are those received whilst working with the University of Glasgow.
- Structure and activity of trypanosomatid metacaspases
Wellcome Trust
2010 - 2014