Dr John Pediani

  • Research Technologist (Molecular Biosciences)

telephone: 0141 330 8494
email: John.Pediani@glasgow.ac.uk

Translational Pharmacology Group, Advanced Research Centre, Level 4/Office 490/Lab 441, 11 Chapel Lane, University of Glasgow, G11 6EW

Import to contacts

ORCID iDhttps://orcid.org/0000-0001-6615-537X

Biography

Key Skills 

Core Funded

  • 2 & 3D multichannel time-lapse fluorescence imaging including colocalisation correlation analysis, surface area volume and fluorescent intensity measurements using Zeiss 980 confocal equipped with the super-resolution Airyscan II detector. 
  • Point spread function and channel chromatic shift aberration analysis to quantify the degree of blur within a microscope system. 
  • Ratiometric intramolecular and intermolecular FRET imaging and analysis using widefield, confocal or single molecule microscopy techniques.
  • Time resolved FRET and BRET plate reader acquisition and analysis. 
  • Confocal acceptor photobleaching and lambda spectral FRET imaging and analysis.
  • FRAP microscopy to determine the diffusion rate of fluorescently tagged proteins.
  • FIF, SpIDA analysis of confocal images to determine the spatial brightness dependent homo-oligomeric state of receptor proteins.
  • Fura 2, Fura 8 AM and redox potential ratiometric imaging. Fluo 8 AM calcium imaging.
  • Super-resolution analysis software (LAMA, ThunderSTORM) to identify single molecule coordinates to generate a localisation x,y list as an input file to generate a sub-diffraction spatial super-resolution image.
  • Single molecule tracking software (PIF, Utrack) to identify single molecule receptor protein association and dissociation. Spot intensity trajectory distribution analysis using GMiMPro & OriginPro software to quantify the homo-oligomeric size of receptor proteins. Step event bleaching analysis of spot trajectories to quantify the sub unit size of proteins using PIF software).
  • Software thresholding tools, (manual and automatic), to quantify information contained in fluorescence images.
  • Deconvolution software techniques (Auotoquant X) to remove out of focus light to resolve structures more clearly.
  • Fluorescence Correlation Spectoscopy (FCS) advice. How to quantify diffusion coefficient and molecular brightness values.
  • Fluorecence Life Time imaging advice how to quantify donor fluophore decay half life values for FRET efficiency measurement. 
  • Light-sheet microscopy advice on imaging set ups and required data storage to image organs or whole animals.

Research interests

I have been interested in fluorescence microscopy since 1989 when I first started to use the ratiometric calcium indicator FURA 2 AM to monitor changes in the intracellular free calcium concentration of cells upon ligand receptor activation. My main interest now is super-resolution microscopy techniques that can be used to quantify the homo- and hetero-oligomerisation state of fluorescing receptor proteins in living cells before and after receptor protein stimulation using drugs which have different potency and efficacy. The weblink below summarises nicely the novel technology that I am particularly interested in which offers a 10x and 100x improvement in resolution and 100x faster image acquisition compared to other super-resolution and confocal techniques which use camera based detectors:

 https://abberior.rocks/superresolution-confocal-systems/minflux/

Publications

List by: Type | Date

Jump to: 2022 | 2021 | 2019 | 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2007 | 2006 | 2003 | 2002 | 2000 | 1999 | 1998 | 1991
Number of items: 39.

2022

Marsango, S. et al. (2022) The M1 muscarinic receptor is present in situ as a ligand-regulated mixture of monomers and oligomeric complexes. Proceedings of the National Academy of Sciences of the United States of America, 119(24), e220110311. (doi: 10.1073/pnas.2201103119) (PMID:35671422) (PMCID:PMC9214538)

2021

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Marsango, Sara, Jolly, Richard, Stoneman, Michael R., Biener, Gabriel, Handel, Tracy M., Raicu, Valerica and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2021) Chemokine receptor CXCR4 oligomerization is disrupted selectively by the antagonist ligand IT1t. Journal of Biological Chemistry, 296, 100139. (doi: 10.1074/jbc.RA120.016612) (PMID:33268380)

2019

Stoneman, Michael R., Biener, Gabriel, Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Badu, Dammar, Eis, Annie, Popa, Ionel, Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 and Raicu, Valerică (2019) A general method to quantify ligand-driven oligomerization from fluorescence-based images. Nature Methods, 16(6), pp. 493-496. (doi: 10.1038/s41592-019-0408-9) (PMID:31110281)

2018

Liu, Y. et al. (2018) Visualization of the activation of the histamine H3 receptor (H3R) using novel fluorescence resonance energy transfer biosensors and their potential application to the study of H3R pharmacology. FEBS Journal, 285(12), pp. 2319-2336. (doi: 10.1111/febs.14484) (PMID:29701013)

Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Ward, Richard J., Marsango, Sara and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2018) Spatial intensity distribution analysis: studies of G Protein-coupled receptor oligomerization. Trends in Pharmacological Sciences, 39(2), pp. 175-186. (doi: 10.1016/j.tips.2017.09.001) (PMID:29032835) (PMCID:PMC5783713)

2017

Ward, Richard J., Marsango, Sara, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2017) The use of spatial intensity distribution analysis to examine G protein-coupled receptor oligomerization. In: Herrick-Davis, Katharine, Milligan, Graeme and Di Giovanni, Giuseppe (eds.) G-Protein-Coupled Receptor Dimers. Series: Receptors (33). Humana Press: Cham, pp. 15-38. ISBN 9783319601724 (doi: 10.1007/978-3-319-60174-8_2)

Shaw, Andy, Jeromson, Stewart, Watterson, Kenneth R. ORCID logoORCID: https://orcid.org/0000-0003-3819-4026, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Gallagher, Iain, Whalley, Tim, Dreczkowski, Gillian, Brooks, Naomi, Galloway, Stuart and Hamilton, D. Lee (2017) Multiple AMPK activators inhibit L-Carnitine uptake in C2C12 skeletal muscle myotubes. American Journal of Physiology: Cell Physiology, 312(6), C689-C696. (doi: 10.1152/ajpcell.00026.2016) (PMID:28298333)

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Harikumar, Kaleeckal G., Miller, Laurence J. and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2017) Spatial intensity distribution analysis quantifies the extent and regulation of homodimerization of the secretin receptor. Biochemical Journal, 474(11), pp. 1879-1895. (doi: 10.1042/BCJ20170184) (PMID:28424368) (PMCID:PMC5442643)

Marsango, Sara, Caltabiano, Gianluigi, Jiménez-Rosés, Mireia, Millan, Mark J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Ward, Richard J. and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2017) A molecular basis for selective antagonist destabilization of dopamine D3 receptor quaternary organization. Scientific Reports, 7, 2134. (doi: 10.1038/s41598-017-02249-3) (PMID:28522847) (PMCID:PMC5437050)

2016

Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Ward, Richard J., Godin, Antoine G., Marsango, Sara and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2016) Dynamic regulation of quaternary organization of the M1 muscarinic receptor by subtype-selective antagonist drugs. Journal of Biological Chemistry, 291, pp. 13132-13146. (doi: 10.1074/jbc.M115.712562) (PMID:27080256) (PMCID:PMC4933229)

2015

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Godin, Antoine G. and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2015) Regulation of oligomeric organization of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor observed by spatial intensity distribution analysis. Journal of Biological Chemistry, 290(20), pp. 12844-12857. (doi: 10.1074/jbc.M115.644724) (PMID:25825490) (PMCID:PMC4432300)

2014

Zakrys, Linas, Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Godin, Antoine G., Graham, Gerard J. ORCID logoORCID: https://orcid.org/0000-0002-7801-204X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2014) Roundabout 1 exists predominantly as a basal dimeric complex and this is unaffected by binding of the ligand Slit2. Biochemical Journal, 461(1), pp. 61-73. (doi: 10.1042/BJ20140190)

2013

Horobin, R.W. ORCID logoORCID: https://orcid.org/0000-0002-6612-6664, Rashid-Doubell, F., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2013) Predicting small molecule fluorescent probe localization in living cells using QSAR modeling. 1. Overview and models for probes of structure, properties and function in single cells. Biotechnic and Histochemistry, 88(8), pp. 440-460. (doi: 10.3109/10520295.2013.780634)

Hudson, B.D. ORCID logoORCID: https://orcid.org/0000-0001-7059-0091, Shimpukade, B., Mackenzie, A.E., Butcher, A.J., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Christiansen, E., Heathcote, H., Tobin, A.B., Ulven, T. and Milligan, G. ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2013) The pharmacology of TUG-891, a potent and selective agonist of the free fatty acid receptor 4 (FFA4/GPR120), demonstrates both potential opportunity and possible challenges to therapeutic agonism. Molecular Pharmacology, 84(5), pp. 710-725. (doi: 10.1124/mol.113.087783)

Segura, V., Pérez-Aso, M., Montó, F., Carceller, E., Noguera, M.A., Pediani, J. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Milligan, G. ORCID logoORCID: https://orcid.org/0000-0002-6946-3519, McGrath, I.C. ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 and D’Ocon, P. (2013) Differences in the signaling pathways of α1A- and α1B-adrenoceptors are related to different endosomal targeting. PLoS ONE, 8(5), e64996. (doi: 10.1371/journal.pone.0064996) (PMID:23717684) (PMCID:PMC3663791)

2012

Wiejak, Jolanta, Dunlop, Julia ORCID logoORCID: https://orcid.org/0000-0003-3180-6763, Stoyle, Chloe, Lappin, Gillian, McIlroy, Anna, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Gao, Shan and Yarwood, Stephen J. (2012) The protein kinase C inhibitor, Ro-31-7459, is a potent activator of ERK and JNK MAP kinases in HUVECs and yet inhibits cyclic AMP-stimulated SOCS-3 gene induction through inactivation of the transcription factor c-Jun. Cellular Signalling, 24(8), pp. 1690-1699. (doi: 10.1016/j.cellsig.2012.04.016)

Cunningham, Margaret R., McIntosh, Kathryn A., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Robben, Joris, Cooke, Alexandra E., Nilsson, Mary, Gould, Gwyn W. ORCID logoORCID: https://orcid.org/0000-0001-6571-2875, Mundell, Stuart, Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 and Plevin, Robin (2012) Novel role for proteinase-activated receptor 2 (PAR2) in membrane trafficking of proteinase-activated receptor 4 (PAR4). Journal of Biological Chemistry, 287(20), pp. 16656-16669. (doi: 10.1074/jbc.M111.315911)

Xu, Tian-Rui, Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2012) Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation. Journal of Biological Chemistry, 287, pp. 14937-14949. (doi: 10.1074/jbc.M111.334300)

Fewou, Simon N., Rupp, Angie, Nickolay, Lauren E., Carrick, Kathryn, Greenshields, Kay N., Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Plomp, Jaap J. and Willison, Hugh J. ORCID logoORCID: https://orcid.org/0000-0002-5997-1683 (2012) Anti-ganglioside antibody internalization attenuates motor nerve terminal injury in a mouse model of acute motor axonal neuropathy. Journal of Clinical Investigation, 122(3), pp. 1037-1051. (doi: 10.1172/JCI59110) (PMID:22307327) (PMCID:PMC3287221)

2011

Alvarez-Curto, Elisa ORCID logoORCID: https://orcid.org/0000-0003-4004-4545, Prihandoko, Rudi, Tautermann, Christofer S., Zwier, Jurriaan M., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Lohse, Martin J., Hoffmann, Carsten, Tobin, Andrew B. and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2011) Developing chemical genetic approaches to explore G protein-coupled receptor function: validation of the use of a Receptor Activated Solely by Synthetic Ligand (RASSL). Molecular Pharmacology, 80(6), pp. 1033-1046. (doi: 10.1124/mol.111.074674) (PMID:21880827) (PMCID:PMC3228535)

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2011) Heteromultimerization of cannabinoid CB1 receptor and orexin OX1 receptor generates a unique complex in which both protomers are regulated by orexin A. Journal of Biological Chemistry, 286(43), pp. 37414-37428. (doi: 10.1074/jbc.M111.287649)

Xu, Tian‑Rui, Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2011) The orexin OX1 receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands. Biochemical Journal, 439(1), pp. 171-183. (doi: 10.1042/BJ20110230)

Van Lith, Marcel, Tiwari, Shweta, Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 and Bulleid, Neil J. ORCID logoORCID: https://orcid.org/0000-0002-9839-5279 (2011) Real-time monitoring of redox changes in the mammalian endoplasmic reticulum. Journal of Cell Science, 124(14), pp. 2349-2356. (doi: 10.1242/jcs.085530)

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2011) Ligand-induced internalisation of the orexin OX1 and cannabinoid CB1 receptors assessed via N-terminal SNAP and CLIP-tagging. British Journal of Pharmacology, 162(6), pp. 1439-1452. (doi: 10.1111/j.1476-5381.2010.01156.x)

2010

Alvarez-Curto, Elisa ORCID logoORCID: https://orcid.org/0000-0003-4004-4545, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2010) Applications of fluorescence and bioluminescence resonance energy transfer to drug discovery at G protein coupled receptors. Analytical and Bioanalytical Chemistry, 398(1), pp. 167-180. (doi: 10.1007/s00216-010-3823-4) (PMID:20517598)

Alvarez-Curto, Elisa, Ward, Richard J., Pediani, John D. and Milligan, Graeme (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry, 285(30), pp. 23318-23330. (doi: 10.1074/jbc.M110.122184) (PMID:20489201) (PMCID:PMC2906324)

2009

Greenshields, K.N. et al. (2009) The neuropathic potential of anti-GM1 autoantibodies is regulated by the local glycolipid environment in mice. Journal of Clinical Investigation, 119(3), pp. 595-610. (doi: 10.1172/JCI37338)

2007

Lopez-Gimenez, Juan F., Canals Buj, Meritxell, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2007) The 1b-adrenoceptor exists as a higher-order oligomer: effective oligomerization is required for receptor maturation, surface delivery, and function. Molecular Pharmacology, 71(4), pp. 1015-1029. (doi: 10.1124/mol.106.033035)

McGrath, I., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Methven, L., MacMillan, J. and Daly, C.J. ORCID logoORCID: https://orcid.org/0000-0002-0410-7548 (2007) Visualisation of Receptors in Native Tissue. Life Sciences 2007, 2007.

Sartania, Nana ORCID logoORCID: https://orcid.org/0000-0002-3196-2312, Appelbe, Shirley, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme (2007) Agonist occupancy of a single monomeric element is sufficient to cause internalization of the dimeric beta(2)-adrenoceptor. Cellular Signalling, 19, pp. 1928-1938. (doi: 10.1016/j.cellsig.2007.05.002)

2006

Ellis, J., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Canals Buj, M., Milasta, S. and Milligan, G. ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2006) Orexin-1 receptor-cannabinoid CB1 receptor heterodimerization results in both ligand-dependent and -independent coordinated alterations of receptor localization and function. Journal of Biological Chemistry, 281(25), pp. 38812-38824. (doi: 10.1074/jbc.M602494200)

2003

Colston, J., Horobin, R.W. ORCID logoORCID: https://orcid.org/0000-0002-6612-6664, Rashid-Doubell, F., Pediani, J. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Johal, K.K. (2003) Why fluorescent probes for endoplasmic reticulum are selective: an experimental and QSAR-modelling study. Biotechnic and Histochemistry, 78(6), pp. 323-332. (doi: 10.1080/10520290310001646659) (PMID:15473580)

2002

Daly, Craig ORCID logoORCID: https://orcid.org/0000-0002-0410-7548, Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, J.F. and McGrath, John ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (2002) Visualising agonist and antagonist interactions: the value of fluorescent ligands. Pharmacologist, 44(2), A73.

2000

Pediani, John Daniel ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, Janet Fraser, Heeley, Robert Paul, Daly, Craig James ORCID logoORCID: https://orcid.org/0000-0002-0410-7548 and McGrath, John Christie ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (2000) Single-cell recombinant pharmacology: bovine α1a-adrenoceptors in rat-1 fibroblasts release intracellular Ca2+, display subtype-characteristic agonism and antagonism, and exhibit an antagonist-reversible inverse concentration-response phase. Journal of Pharmacology and Experimental Therapeutics, 293(3), pp. 887-895.

Mackenzie, J.F., Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Daly, Craig ORCID logoORCID: https://orcid.org/0000-0002-0410-7548, Deighan, C. and McGrath, John ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (2000) Fluorescent ligand binding to alpha1-adrenoceptors in murine hepatocytes. In: 10th Porto Meeting on Adrenergic Mechanisms, Porto, Portugal, 25-27 Sept 2000, P2.

1999

McGrath, J.C. ORCID logoORCID: https://orcid.org/0000-0002-5276-0381, Nagadeh, M.A., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, J.F. and Daly, C.J. ORCID logoORCID: https://orcid.org/0000-0002-0410-7548 (1999) Importance of agonists in α-adrenoceptor classification and localisation of α1-adrenoceptors in human prostate. European Urology, 36(S1), pp. 80-88. (doi: 10.1159/000052326)

1998

Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, J.F., Daly, C.J. and McGrath, J.C. ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (1998) Functional pharmacological properties of the cloned bovine a 1a-adrenoceptor (a 1a-AR) stably expressed in rat-1 fibroblasts. Naunyn-Schmiedeberg's Archives of Pharmacology, 358(Supp.2), p. 646.

Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, J.F., Daly, Craig ORCID logoORCID: https://orcid.org/0000-0002-0410-7548 and McGrath, John ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (1998) Comparison of functional and binding pharmacological properties of the cloned bovine a 1a-adrenoceptor (a 1a-AR) stably expressed in rat-1 fibroblasts. Naunyn-Schmiedeberg's Archives of Pharmacology, 358(S2), P6.47.

1991

Wilson, S.M., Pediani, J. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Cockburn, F., Bovell, D.L., Jenkinson, D.M., Paton, J.Y. ORCID logoORCID: https://orcid.org/0000-0002-9896-7855, Coutts, J., Davidson, R., Lambert, J. and Morris, G. (1991) The cholinergic regulation of potassium (86Rb ) permeability in sweat glands isolated from patients with cystic fibrosis. Experimental Physiology, 76(4), pp. 573-578.

This list was generated on Sun Jun 15 06:53:51 2025 BST.
Jump to: Articles | Book Sections | Conference or Workshop Item | Conference Proceedings
Number of items: 39.

Articles

Marsango, S. et al. (2022) The M1 muscarinic receptor is present in situ as a ligand-regulated mixture of monomers and oligomeric complexes. Proceedings of the National Academy of Sciences of the United States of America, 119(24), e220110311. (doi: 10.1073/pnas.2201103119) (PMID:35671422) (PMCID:PMC9214538)

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Marsango, Sara, Jolly, Richard, Stoneman, Michael R., Biener, Gabriel, Handel, Tracy M., Raicu, Valerica and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2021) Chemokine receptor CXCR4 oligomerization is disrupted selectively by the antagonist ligand IT1t. Journal of Biological Chemistry, 296, 100139. (doi: 10.1074/jbc.RA120.016612) (PMID:33268380)

Stoneman, Michael R., Biener, Gabriel, Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Badu, Dammar, Eis, Annie, Popa, Ionel, Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 and Raicu, Valerică (2019) A general method to quantify ligand-driven oligomerization from fluorescence-based images. Nature Methods, 16(6), pp. 493-496. (doi: 10.1038/s41592-019-0408-9) (PMID:31110281)

Liu, Y. et al. (2018) Visualization of the activation of the histamine H3 receptor (H3R) using novel fluorescence resonance energy transfer biosensors and their potential application to the study of H3R pharmacology. FEBS Journal, 285(12), pp. 2319-2336. (doi: 10.1111/febs.14484) (PMID:29701013)

Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Ward, Richard J., Marsango, Sara and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2018) Spatial intensity distribution analysis: studies of G Protein-coupled receptor oligomerization. Trends in Pharmacological Sciences, 39(2), pp. 175-186. (doi: 10.1016/j.tips.2017.09.001) (PMID:29032835) (PMCID:PMC5783713)

Shaw, Andy, Jeromson, Stewart, Watterson, Kenneth R. ORCID logoORCID: https://orcid.org/0000-0003-3819-4026, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Gallagher, Iain, Whalley, Tim, Dreczkowski, Gillian, Brooks, Naomi, Galloway, Stuart and Hamilton, D. Lee (2017) Multiple AMPK activators inhibit L-Carnitine uptake in C2C12 skeletal muscle myotubes. American Journal of Physiology: Cell Physiology, 312(6), C689-C696. (doi: 10.1152/ajpcell.00026.2016) (PMID:28298333)

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Harikumar, Kaleeckal G., Miller, Laurence J. and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2017) Spatial intensity distribution analysis quantifies the extent and regulation of homodimerization of the secretin receptor. Biochemical Journal, 474(11), pp. 1879-1895. (doi: 10.1042/BCJ20170184) (PMID:28424368) (PMCID:PMC5442643)

Marsango, Sara, Caltabiano, Gianluigi, Jiménez-Rosés, Mireia, Millan, Mark J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Ward, Richard J. and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2017) A molecular basis for selective antagonist destabilization of dopamine D3 receptor quaternary organization. Scientific Reports, 7, 2134. (doi: 10.1038/s41598-017-02249-3) (PMID:28522847) (PMCID:PMC5437050)

Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Ward, Richard J., Godin, Antoine G., Marsango, Sara and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2016) Dynamic regulation of quaternary organization of the M1 muscarinic receptor by subtype-selective antagonist drugs. Journal of Biological Chemistry, 291, pp. 13132-13146. (doi: 10.1074/jbc.M115.712562) (PMID:27080256) (PMCID:PMC4933229)

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Godin, Antoine G. and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2015) Regulation of oligomeric organization of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor observed by spatial intensity distribution analysis. Journal of Biological Chemistry, 290(20), pp. 12844-12857. (doi: 10.1074/jbc.M115.644724) (PMID:25825490) (PMCID:PMC4432300)

Zakrys, Linas, Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Godin, Antoine G., Graham, Gerard J. ORCID logoORCID: https://orcid.org/0000-0002-7801-204X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2014) Roundabout 1 exists predominantly as a basal dimeric complex and this is unaffected by binding of the ligand Slit2. Biochemical Journal, 461(1), pp. 61-73. (doi: 10.1042/BJ20140190)

Horobin, R.W. ORCID logoORCID: https://orcid.org/0000-0002-6612-6664, Rashid-Doubell, F., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2013) Predicting small molecule fluorescent probe localization in living cells using QSAR modeling. 1. Overview and models for probes of structure, properties and function in single cells. Biotechnic and Histochemistry, 88(8), pp. 440-460. (doi: 10.3109/10520295.2013.780634)

Hudson, B.D. ORCID logoORCID: https://orcid.org/0000-0001-7059-0091, Shimpukade, B., Mackenzie, A.E., Butcher, A.J., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Christiansen, E., Heathcote, H., Tobin, A.B., Ulven, T. and Milligan, G. ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2013) The pharmacology of TUG-891, a potent and selective agonist of the free fatty acid receptor 4 (FFA4/GPR120), demonstrates both potential opportunity and possible challenges to therapeutic agonism. Molecular Pharmacology, 84(5), pp. 710-725. (doi: 10.1124/mol.113.087783)

Segura, V., Pérez-Aso, M., Montó, F., Carceller, E., Noguera, M.A., Pediani, J. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Milligan, G. ORCID logoORCID: https://orcid.org/0000-0002-6946-3519, McGrath, I.C. ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 and D’Ocon, P. (2013) Differences in the signaling pathways of α1A- and α1B-adrenoceptors are related to different endosomal targeting. PLoS ONE, 8(5), e64996. (doi: 10.1371/journal.pone.0064996) (PMID:23717684) (PMCID:PMC3663791)

Wiejak, Jolanta, Dunlop, Julia ORCID logoORCID: https://orcid.org/0000-0003-3180-6763, Stoyle, Chloe, Lappin, Gillian, McIlroy, Anna, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Gao, Shan and Yarwood, Stephen J. (2012) The protein kinase C inhibitor, Ro-31-7459, is a potent activator of ERK and JNK MAP kinases in HUVECs and yet inhibits cyclic AMP-stimulated SOCS-3 gene induction through inactivation of the transcription factor c-Jun. Cellular Signalling, 24(8), pp. 1690-1699. (doi: 10.1016/j.cellsig.2012.04.016)

Cunningham, Margaret R., McIntosh, Kathryn A., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Robben, Joris, Cooke, Alexandra E., Nilsson, Mary, Gould, Gwyn W. ORCID logoORCID: https://orcid.org/0000-0001-6571-2875, Mundell, Stuart, Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 and Plevin, Robin (2012) Novel role for proteinase-activated receptor 2 (PAR2) in membrane trafficking of proteinase-activated receptor 4 (PAR4). Journal of Biological Chemistry, 287(20), pp. 16656-16669. (doi: 10.1074/jbc.M111.315911)

Xu, Tian-Rui, Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2012) Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation. Journal of Biological Chemistry, 287, pp. 14937-14949. (doi: 10.1074/jbc.M111.334300)

Fewou, Simon N., Rupp, Angie, Nickolay, Lauren E., Carrick, Kathryn, Greenshields, Kay N., Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Plomp, Jaap J. and Willison, Hugh J. ORCID logoORCID: https://orcid.org/0000-0002-5997-1683 (2012) Anti-ganglioside antibody internalization attenuates motor nerve terminal injury in a mouse model of acute motor axonal neuropathy. Journal of Clinical Investigation, 122(3), pp. 1037-1051. (doi: 10.1172/JCI59110) (PMID:22307327) (PMCID:PMC3287221)

Alvarez-Curto, Elisa ORCID logoORCID: https://orcid.org/0000-0003-4004-4545, Prihandoko, Rudi, Tautermann, Christofer S., Zwier, Jurriaan M., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Lohse, Martin J., Hoffmann, Carsten, Tobin, Andrew B. and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2011) Developing chemical genetic approaches to explore G protein-coupled receptor function: validation of the use of a Receptor Activated Solely by Synthetic Ligand (RASSL). Molecular Pharmacology, 80(6), pp. 1033-1046. (doi: 10.1124/mol.111.074674) (PMID:21880827) (PMCID:PMC3228535)

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2011) Heteromultimerization of cannabinoid CB1 receptor and orexin OX1 receptor generates a unique complex in which both protomers are regulated by orexin A. Journal of Biological Chemistry, 286(43), pp. 37414-37428. (doi: 10.1074/jbc.M111.287649)

Xu, Tian‑Rui, Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2011) The orexin OX1 receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands. Biochemical Journal, 439(1), pp. 171-183. (doi: 10.1042/BJ20110230)

Van Lith, Marcel, Tiwari, Shweta, Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 and Bulleid, Neil J. ORCID logoORCID: https://orcid.org/0000-0002-9839-5279 (2011) Real-time monitoring of redox changes in the mammalian endoplasmic reticulum. Journal of Cell Science, 124(14), pp. 2349-2356. (doi: 10.1242/jcs.085530)

Ward, Richard J., Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2011) Ligand-induced internalisation of the orexin OX1 and cannabinoid CB1 receptors assessed via N-terminal SNAP and CLIP-tagging. British Journal of Pharmacology, 162(6), pp. 1439-1452. (doi: 10.1111/j.1476-5381.2010.01156.x)

Alvarez-Curto, Elisa ORCID logoORCID: https://orcid.org/0000-0003-4004-4545, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2010) Applications of fluorescence and bioluminescence resonance energy transfer to drug discovery at G protein coupled receptors. Analytical and Bioanalytical Chemistry, 398(1), pp. 167-180. (doi: 10.1007/s00216-010-3823-4) (PMID:20517598)

Alvarez-Curto, Elisa, Ward, Richard J., Pediani, John D. and Milligan, Graeme (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry, 285(30), pp. 23318-23330. (doi: 10.1074/jbc.M110.122184) (PMID:20489201) (PMCID:PMC2906324)

Greenshields, K.N. et al. (2009) The neuropathic potential of anti-GM1 autoantibodies is regulated by the local glycolipid environment in mice. Journal of Clinical Investigation, 119(3), pp. 595-610. (doi: 10.1172/JCI37338)

Lopez-Gimenez, Juan F., Canals Buj, Meritxell, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2007) The 1b-adrenoceptor exists as a higher-order oligomer: effective oligomerization is required for receptor maturation, surface delivery, and function. Molecular Pharmacology, 71(4), pp. 1015-1029. (doi: 10.1124/mol.106.033035)

Sartania, Nana ORCID logoORCID: https://orcid.org/0000-0002-3196-2312, Appelbe, Shirley, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme (2007) Agonist occupancy of a single monomeric element is sufficient to cause internalization of the dimeric beta(2)-adrenoceptor. Cellular Signalling, 19, pp. 1928-1938. (doi: 10.1016/j.cellsig.2007.05.002)

Ellis, J., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Canals Buj, M., Milasta, S. and Milligan, G. ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2006) Orexin-1 receptor-cannabinoid CB1 receptor heterodimerization results in both ligand-dependent and -independent coordinated alterations of receptor localization and function. Journal of Biological Chemistry, 281(25), pp. 38812-38824. (doi: 10.1074/jbc.M602494200)

Colston, J., Horobin, R.W. ORCID logoORCID: https://orcid.org/0000-0002-6612-6664, Rashid-Doubell, F., Pediani, J. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Johal, K.K. (2003) Why fluorescent probes for endoplasmic reticulum are selective: an experimental and QSAR-modelling study. Biotechnic and Histochemistry, 78(6), pp. 323-332. (doi: 10.1080/10520290310001646659) (PMID:15473580)

Daly, Craig ORCID logoORCID: https://orcid.org/0000-0002-0410-7548, Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, J.F. and McGrath, John ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (2002) Visualising agonist and antagonist interactions: the value of fluorescent ligands. Pharmacologist, 44(2), A73.

Pediani, John Daniel ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, Janet Fraser, Heeley, Robert Paul, Daly, Craig James ORCID logoORCID: https://orcid.org/0000-0002-0410-7548 and McGrath, John Christie ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (2000) Single-cell recombinant pharmacology: bovine α1a-adrenoceptors in rat-1 fibroblasts release intracellular Ca2+, display subtype-characteristic agonism and antagonism, and exhibit an antagonist-reversible inverse concentration-response phase. Journal of Pharmacology and Experimental Therapeutics, 293(3), pp. 887-895.

McGrath, J.C. ORCID logoORCID: https://orcid.org/0000-0002-5276-0381, Nagadeh, M.A., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, J.F. and Daly, C.J. ORCID logoORCID: https://orcid.org/0000-0002-0410-7548 (1999) Importance of agonists in α-adrenoceptor classification and localisation of α1-adrenoceptors in human prostate. European Urology, 36(S1), pp. 80-88. (doi: 10.1159/000052326)

Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, J.F., Daly, C.J. and McGrath, J.C. ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (1998) Functional pharmacological properties of the cloned bovine a 1a-adrenoceptor (a 1a-AR) stably expressed in rat-1 fibroblasts. Naunyn-Schmiedeberg's Archives of Pharmacology, 358(Supp.2), p. 646.

Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Mackenzie, J.F., Daly, Craig ORCID logoORCID: https://orcid.org/0000-0002-0410-7548 and McGrath, John ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (1998) Comparison of functional and binding pharmacological properties of the cloned bovine a 1a-adrenoceptor (a 1a-AR) stably expressed in rat-1 fibroblasts. Naunyn-Schmiedeberg's Archives of Pharmacology, 358(S2), P6.47.

Wilson, S.M., Pediani, J. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Cockburn, F., Bovell, D.L., Jenkinson, D.M., Paton, J.Y. ORCID logoORCID: https://orcid.org/0000-0002-9896-7855, Coutts, J., Davidson, R., Lambert, J. and Morris, G. (1991) The cholinergic regulation of potassium (86Rb ) permeability in sweat glands isolated from patients with cystic fibrosis. Experimental Physiology, 76(4), pp. 573-578.

Book Sections

Ward, Richard J., Marsango, Sara, Pediani, John D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X and Milligan, Graeme ORCID logoORCID: https://orcid.org/0000-0002-6946-3519 (2017) The use of spatial intensity distribution analysis to examine G protein-coupled receptor oligomerization. In: Herrick-Davis, Katharine, Milligan, Graeme and Di Giovanni, Giuseppe (eds.) G-Protein-Coupled Receptor Dimers. Series: Receptors (33). Humana Press: Cham, pp. 15-38. ISBN 9783319601724 (doi: 10.1007/978-3-319-60174-8_2)

Conference or Workshop Item

McGrath, I., Pediani, J.D. ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Methven, L., MacMillan, J. and Daly, C.J. ORCID logoORCID: https://orcid.org/0000-0002-0410-7548 (2007) Visualisation of Receptors in Native Tissue. Life Sciences 2007, 2007.

Conference Proceedings

Mackenzie, J.F., Pediani, John ORCID logoORCID: https://orcid.org/0000-0001-6615-537X, Daly, Craig ORCID logoORCID: https://orcid.org/0000-0002-0410-7548, Deighan, C. and McGrath, John ORCID logoORCID: https://orcid.org/0000-0002-5276-0381 (2000) Fluorescent ligand binding to alpha1-adrenoceptors in murine hepatocytes. In: 10th Porto Meeting on Adrenergic Mechanisms, Porto, Portugal, 25-27 Sept 2000, P2.

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Research datasets

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Number of items: 1.

2022

Marsango, S., Jenkins, L. , Pediani, J. , Bradley, S. , Ward, R., Hesse, S. and Milligan, G. (2022) The M1 muscarinic receptor is present in situ as a ligand-regulated mixture of monomers and oligomeric complexes. [Data Collection]

This list was generated on Sun Jun 15 18:44:12 2025 BST.

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