Dr Elisa Alvarez-Curto

  • Lecturer in Pharmacology (Life Sciences Human Life Sciences)

email: Elisa.Alvarez-Curto@glasgow.ac.uk

R253 Level 2, Pharmacology Research Theme, Wolfson Link Building, Glasgow G12 8QQ

ORCID iDhttps://orcid.org/0000-0003-4004-4545

Biography

Dr Elisa Alvarez-Curto studied for her undergraduate degree in her homeland of Spain prior to a move Leiden in the The Netherlands to complete a PhD in Developmental Biology and Cell Signalling.

A year into the PhD, Elisa's supervisor relocated to the University of Dundee, and it was then that she came to the realisation that, although it might not be a requirement, it is important to be resilient and prepared to adapt to change, particularly on the earlier stages of your career.

She subsequently moved to the University of Glasgow in 2007 and worked as a Postdoctoral Researcher in Molecular Pharmacology until early 2020, during which time her research focused on understanding the molecular pharmacology of G protein-coupled receptors in health and disease.

On 8 April 2020, Elisa was seconded together with a handful of University of Glasgow volunteers to help set up the first high-throughput COVID-19 clinical testing lab in Scotland, which later became the Lighthouse Lab in Glasgow (LLiG). Within 20 days of the lab's inception, the team were processing live samples and reporting results to the public. This had a major impact on Elisa, who came to the realisation for the first time that her skills as a scientist could have a positive and direct impact in society.

After just over a year working at LLiG, she joined the School of Life Sciences as Lecturer in Pharmacology in September 2021 where she develop and teach different areas of the Molecular Pharmacology curriculum.

Research interests

Dr Alvarez-Curto's principal research interests lie in the study of the structure and function of G protein-coupled receptors (GPCRs) using in vitro molecular pharmacology and cell signalling methods as well as in vivo pharmacology approaches.

Parts of that research have focused in investigating how ligand induced changes to the tertiary and quaternary structure of GPCRs alter their pharmacology and how this may be used as potential therapeutic target. Dissecting the changes in the conformations of GPCRs at a molecular level and subsequent signalling outcomes have helped further understanding the biology of this class of receptors.

Over the course of her career, Elisa has developed a depth of knowledge on applying molecular and biophysical methods to the study of receptor pharmacology using biosensors and other molecular techniques (mutagenesis, CRISPR) and has applied cutting edge technologies such as label-free analysis of GPCR mediated cellular responses to drugs and compounds.

In recent years, she has engaged her research in the interactions between metabolism and immunity particularly focusing on the role of free-fatty acid receptors in metabolic conditions such as Type 2 diabetes and metabolic syndrome using both in vitro and in vivo models.

Publications

List by: Type | Date

Jump to: 2020 | 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2011 | 2010
Number of items: 20.

2020

Prihandoko, R. et al. (2020) Pathophysiological regulation of lung function by the free fatty acid receptor FFA4. Science Translational Medicine, 12(557), eaaw9009. (doi: 10.1126/scitranslmed.aaw9009) (PMID:32817367)

2018

Marsango, S., Ward, R. J., Alvarez-Curto, E. and Milligan, G. (2018) Muscarinic receptor oligomerization. Neuropharmacology, 136(Part C), pp. 401-410. (doi: 10.1016/j.neuropharm.2017.11.023) (PMID:29146505) (PMCID:PMC6078712)

2017

Viskaitis, P. et al. (2017) Modulation of SF1 neuron activity coordinately regulates both feeding behaviour and associated emotional states. Cell Reports, 21(12), pp. 3559-3572. (doi: 10.1016/j.celrep.2017.11.089) (PMID:29262334) (PMCID:PMC5746599)

Milligan, G. , Alvarez-Curto, E. , Hudson, B. D. , Prihandoko, R. and Tobin, A. B. (2017) FFA4/GPR120: pharmacology and therapeutic opportunities. Trends in Pharmacological Sciences, 38(9), pp. 809-821. (doi: 10.1016/j.tips.2017.06.006) (PMID:28734639) (PMCID:PMC5582618)

Lopez-Gimenez, J., Alvarez-Curto, E. and Milligan, G. (2017) M3 muscarinic acetylcholine receptor facilitates the endocytosis of mu opioid receptor mediated by morphine independently of the formation of heteromeric complexes. Cellular Signalling, 35, pp. 208-222. (doi: 10.1016/j.cellsig.2017.04.006) (PMID:28411124)

Watterson, K. R. et al. (2017) Probe-dependent negative allosteric modulators of the long-chain free fatty acid receptor FFA4. Molecular Pharmacology, 91(6), pp. 630-641. (doi: 10.1124/mol.116.107821) (PMID:28385906) (PMCID:PMC5438128)

2016

Alvarez-Curto, E. , Inoue, A., Jenkins, L. , Raihan, S. Z., Prihandoko, R., Tobin, A. B. and Milligan, G. (2016) Targeted elimination of G proteins and arrestins defines their specific contributions to both intensity and duration of G protein-coupled receptor signalling. Journal of Biological Chemistry, 291(53), pp. 27147-27159. (doi: 10.1074/jbc.M116.754887) (PMID:27852822) (PMCID:PMC5207144)

Alvarez-Curto, E. and Milligan, G. (2016) Metabolism meets immunity: the role of free fatty acid receptors in the immune system. Biochemical Pharmacology, 114, pp. 3-13. (doi: 10.1016/j.bcp.2016.03.017) (PMID:27002183)

Prihandoko, R., Alvarez-Curto, E. , Hudson, B. D. , Butcher, A. J., Ulven, T., Miller, A. M., Tobin, A. B. and Milligan, G. (2016) Distinct phosphorylation clusters determines the signalling outcome of the free fatty acid receptor FFA4/GPR120. Molecular Pharmacology, 89(5), pp. 505-520. (doi: 10.1124/mol.115.101949) (PMID:26873857)

2015

Milligan, G. , Alvarez-Curto, E. , Watterson, K.R., Ulven, T. and Hudson, B.D. (2015) Characterizing pharmacological ligands to study the long-chain fatty acid receptors GPR40/FFA1 and GPR120/FFA4. British Journal of Pharmacology, 172(13), pp. 3254-3265. (doi: 10.1111/bph.12879) (PMID:25131623)

Aslanoglou, D., Alvarez-Curto, E. , Marsango, S. and Milligan, G. (2015) Distinct agonist regulation of muscarinic acetylcholine M2-M3 heteromers and their corresponding homomers. Journal of Biological Chemistry, 290(23), pp. 14785-14796. (doi: 10.1074/jbc.M115.649079) (PMID:25918156) (PMCID:PMC4505543)

Liste, M. J., Caltabiano, G., Ward, R. J., Alvarez-Curto, E. , Marsango, S. and Milligan, G. (2015) The molecular basis of oligomeric organization of the human m3 muscarinic acetylcholine receptor. Molecular Pharmacology, 87(6), pp. 936-953. (doi: 10.1124/mol.114.096925) (PMID:25769304)

Alvarez-Curto, E. and Milligan, G. (2015) Defining the functional equivalence of wild-type and chemically engineered G protein-coupled receptors. In: Thiel, G. (ed.) Designer Receptors Exclusively Activated by Designer Drugs. Series: Neuromethods (108). Springer New York, pp. 1-28. ISBN 9781493929436 (doi:10.1007/978-1-4939-2944-3_1)

2014

Butcher, A. J., Hudson, B. D. , Shimpukade, B., Alvarez-Curto, E. , Prihandoko, R., Ulven, T., Milligan, G. and Tobin, A. B. (2014) Concomitant action of structural elements and receptor phosphorylation determine arrestin-3 interaction with the free fatty acid receptor FFA4. Journal of Biological Chemistry, 289, pp. 18451-18465. (doi: 10.1074/jbc.M114.568816) (PMID:24817122) (PMCID:PMC4140278)

2013

Patowary, S., Alvarez-Curto, E. , Xu, T.-R., Holz, J.D., Oliver, J.A., Milligan, G. and Raicu, V. (2013) The muscarinic M3acetylcholine receptor exists as two differently sized complexes at the plasma membrane. Biochemical Journal, 452(2), pp. 303-312. (doi: 10.1042/BJ20121902) (PMID:23521066)

2011

Alvarez-Curto, E. , Prihandoko, R., Tautermann, C. S., Zwier, J. M., Pediani, J. D. , Lohse, M. J., Hoffmann, C., Tobin, A. B. and Milligan, G. (2011) Developing chemical genetic approaches to explore G protein-coupled receptor function: validation of the use of a Receptor Activated Solely by Synthetic Ligand (RASSL). Molecular Pharmacology, 80(6), pp. 1033-1046. (doi: 10.1124/mol.111.074674) (PMID:21880827) (PMCID:PMC3228535)

Ward, R.J., Alvarez-Curto, E. and Milligan, G. (2011) Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression. Methods in Molecular Biology, 746, pp. 21-37. (doi: 10.1007/978-1-61779-126-0_2)

2010

Alvarez-Curto, E. , Pediani, J. D. and Milligan, G. (2010) Applications of fluorescence and bioluminescence resonance energy transfer to drug discovery at G protein coupled receptors. Analytical and Bioanalytical Chemistry, 398(1), pp. 167-180. (doi: 10.1007/s00216-010-3823-4) (PMID:20517598)

Alvarez-Curto, E., Ward, R. J., Pediani, J. D. and Milligan, G. (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry, 285(30), pp. 23318-23330. (doi: 10.1074/jbc.M110.122184) (PMID:20489201) (PMCID:PMC2906324)

Jenkins, L. , Alvarez-Curto, E. , Campbell, K., De Munnik, S., Canals Buj, M., Schlyer, S. and Milligan, G. (2010) Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Gα13 and β-arrestin-2. British Journal of Pharmacology, 162(3), pp. 733-748. (doi: 10.1111/j.1476-5381.2010.01082.x)

This list was generated on Thu Oct 21 14:52:02 2021 BST.
Number of items: 20.

Articles

Prihandoko, R. et al. (2020) Pathophysiological regulation of lung function by the free fatty acid receptor FFA4. Science Translational Medicine, 12(557), eaaw9009. (doi: 10.1126/scitranslmed.aaw9009) (PMID:32817367)

Marsango, S., Ward, R. J., Alvarez-Curto, E. and Milligan, G. (2018) Muscarinic receptor oligomerization. Neuropharmacology, 136(Part C), pp. 401-410. (doi: 10.1016/j.neuropharm.2017.11.023) (PMID:29146505) (PMCID:PMC6078712)

Viskaitis, P. et al. (2017) Modulation of SF1 neuron activity coordinately regulates both feeding behaviour and associated emotional states. Cell Reports, 21(12), pp. 3559-3572. (doi: 10.1016/j.celrep.2017.11.089) (PMID:29262334) (PMCID:PMC5746599)

Milligan, G. , Alvarez-Curto, E. , Hudson, B. D. , Prihandoko, R. and Tobin, A. B. (2017) FFA4/GPR120: pharmacology and therapeutic opportunities. Trends in Pharmacological Sciences, 38(9), pp. 809-821. (doi: 10.1016/j.tips.2017.06.006) (PMID:28734639) (PMCID:PMC5582618)

Lopez-Gimenez, J., Alvarez-Curto, E. and Milligan, G. (2017) M3 muscarinic acetylcholine receptor facilitates the endocytosis of mu opioid receptor mediated by morphine independently of the formation of heteromeric complexes. Cellular Signalling, 35, pp. 208-222. (doi: 10.1016/j.cellsig.2017.04.006) (PMID:28411124)

Watterson, K. R. et al. (2017) Probe-dependent negative allosteric modulators of the long-chain free fatty acid receptor FFA4. Molecular Pharmacology, 91(6), pp. 630-641. (doi: 10.1124/mol.116.107821) (PMID:28385906) (PMCID:PMC5438128)

Alvarez-Curto, E. , Inoue, A., Jenkins, L. , Raihan, S. Z., Prihandoko, R., Tobin, A. B. and Milligan, G. (2016) Targeted elimination of G proteins and arrestins defines their specific contributions to both intensity and duration of G protein-coupled receptor signalling. Journal of Biological Chemistry, 291(53), pp. 27147-27159. (doi: 10.1074/jbc.M116.754887) (PMID:27852822) (PMCID:PMC5207144)

Alvarez-Curto, E. and Milligan, G. (2016) Metabolism meets immunity: the role of free fatty acid receptors in the immune system. Biochemical Pharmacology, 114, pp. 3-13. (doi: 10.1016/j.bcp.2016.03.017) (PMID:27002183)

Prihandoko, R., Alvarez-Curto, E. , Hudson, B. D. , Butcher, A. J., Ulven, T., Miller, A. M., Tobin, A. B. and Milligan, G. (2016) Distinct phosphorylation clusters determines the signalling outcome of the free fatty acid receptor FFA4/GPR120. Molecular Pharmacology, 89(5), pp. 505-520. (doi: 10.1124/mol.115.101949) (PMID:26873857)

Milligan, G. , Alvarez-Curto, E. , Watterson, K.R., Ulven, T. and Hudson, B.D. (2015) Characterizing pharmacological ligands to study the long-chain fatty acid receptors GPR40/FFA1 and GPR120/FFA4. British Journal of Pharmacology, 172(13), pp. 3254-3265. (doi: 10.1111/bph.12879) (PMID:25131623)

Aslanoglou, D., Alvarez-Curto, E. , Marsango, S. and Milligan, G. (2015) Distinct agonist regulation of muscarinic acetylcholine M2-M3 heteromers and their corresponding homomers. Journal of Biological Chemistry, 290(23), pp. 14785-14796. (doi: 10.1074/jbc.M115.649079) (PMID:25918156) (PMCID:PMC4505543)

Liste, M. J., Caltabiano, G., Ward, R. J., Alvarez-Curto, E. , Marsango, S. and Milligan, G. (2015) The molecular basis of oligomeric organization of the human m3 muscarinic acetylcholine receptor. Molecular Pharmacology, 87(6), pp. 936-953. (doi: 10.1124/mol.114.096925) (PMID:25769304)

Butcher, A. J., Hudson, B. D. , Shimpukade, B., Alvarez-Curto, E. , Prihandoko, R., Ulven, T., Milligan, G. and Tobin, A. B. (2014) Concomitant action of structural elements and receptor phosphorylation determine arrestin-3 interaction with the free fatty acid receptor FFA4. Journal of Biological Chemistry, 289, pp. 18451-18465. (doi: 10.1074/jbc.M114.568816) (PMID:24817122) (PMCID:PMC4140278)

Patowary, S., Alvarez-Curto, E. , Xu, T.-R., Holz, J.D., Oliver, J.A., Milligan, G. and Raicu, V. (2013) The muscarinic M3acetylcholine receptor exists as two differently sized complexes at the plasma membrane. Biochemical Journal, 452(2), pp. 303-312. (doi: 10.1042/BJ20121902) (PMID:23521066)

Alvarez-Curto, E. , Prihandoko, R., Tautermann, C. S., Zwier, J. M., Pediani, J. D. , Lohse, M. J., Hoffmann, C., Tobin, A. B. and Milligan, G. (2011) Developing chemical genetic approaches to explore G protein-coupled receptor function: validation of the use of a Receptor Activated Solely by Synthetic Ligand (RASSL). Molecular Pharmacology, 80(6), pp. 1033-1046. (doi: 10.1124/mol.111.074674) (PMID:21880827) (PMCID:PMC3228535)

Ward, R.J., Alvarez-Curto, E. and Milligan, G. (2011) Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression. Methods in Molecular Biology, 746, pp. 21-37. (doi: 10.1007/978-1-61779-126-0_2)

Alvarez-Curto, E. , Pediani, J. D. and Milligan, G. (2010) Applications of fluorescence and bioluminescence resonance energy transfer to drug discovery at G protein coupled receptors. Analytical and Bioanalytical Chemistry, 398(1), pp. 167-180. (doi: 10.1007/s00216-010-3823-4) (PMID:20517598)

Alvarez-Curto, E., Ward, R. J., Pediani, J. D. and Milligan, G. (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry, 285(30), pp. 23318-23330. (doi: 10.1074/jbc.M110.122184) (PMID:20489201) (PMCID:PMC2906324)

Jenkins, L. , Alvarez-Curto, E. , Campbell, K., De Munnik, S., Canals Buj, M., Schlyer, S. and Milligan, G. (2010) Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Gα13 and β-arrestin-2. British Journal of Pharmacology, 162(3), pp. 733-748. (doi: 10.1111/j.1476-5381.2010.01082.x)

Book Sections

Alvarez-Curto, E. and Milligan, G. (2015) Defining the functional equivalence of wild-type and chemically engineered G protein-coupled receptors. In: Thiel, G. (ed.) Designer Receptors Exclusively Activated by Designer Drugs. Series: Neuromethods (108). Springer New York, pp. 1-28. ISBN 9781493929436 (doi:10.1007/978-1-4939-2944-3_1)

This list was generated on Thu Oct 21 14:52:02 2021 BST.