Mr Craig Lapsley
- Technician (Parasitology)
telephone:
6069
email:
Craig.Lapsley@glasgow.ac.uk
RB624 Level B6, Iii - Gbrc, University Place, Glasgow G12 8TA
Publications
2025
Damasceno, Jeziel D. ORCID: https://orcid.org/0000-0003-2077-3214, Briggs, Emma M., Krasilnikova, Marija
ORCID: https://orcid.org/0000-0002-9140-9746, Marques, Catarina A., Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2025)
R-loops acted on by RNase H1 influence DNA replication timing and genome stability in Leishmania.
Nature Communications, 16,
1470.
(doi: 10.1038/s41467-025-56785-y)
(PMID:39922816)
(PMCID:PMC11807225)
Krasilnikova, Marija ORCID: https://orcid.org/0000-0002-9140-9746, Marques, Catarina A.
ORCID: https://orcid.org/0000-0003-1324-5448, Briggs, Emma, Lapsley, Craig, Hamilton, Graham, Beraldi, Dario, Crouch, Kathryn
ORCID: https://orcid.org/0000-0001-9310-4762 and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2025)
Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei.
Nature Communications, 16,
751.
(doi: 10.1038/s41467-025-56087-3)
2024
Damasceno, Jeziel ORCID: https://orcid.org/0000-0003-2077-3214, LA. Silva, Gabriel, Marques, Catarina A.
ORCID: https://orcid.org/0000-0003-1324-5448, Krasilnikova, Marija
ORCID: https://orcid.org/0000-0002-9140-9746, Lapsley, Craig, Beraldi, Dario and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2024)
Nuclear DNA replication in Leishmania major relies on a single constitutive origin per chromosome supplemented by thousands of stochastic initiation events.
bioRxiv,
(doi: 10.1101/2024.11.14.623610)
Damasceno, Jeziel D. ORCID: https://orcid.org/0000-0003-2077-3214, Briggs, Emma M., Krasilnikova, Marija
ORCID: https://orcid.org/0000-0002-9140-9746, Marques, Catarina A.
ORCID: https://orcid.org/0000-0003-1324-5448, Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2024)
R-loops acted on by RNase H1 are a determinant of chromosome length-associated DNA replication timing and genome stability in Leishmania.
bioRxiv,
(doi: 10.1101/2024.04.29.591643)
2023
Girasol, M. J. et al. (2023) RAD51-mediated R-loop formation acts to repair transcription associated DNA breaks driving antigenic variation in Trypanosoma brucei. Proceedings of the National Academy of Sciences of the United States of America, 120(48), e230930612. (doi: 10.1073/pnas.2309306120) (PMID:37988471) (PMCID:PMC10691351)
2020
Damasceno, Jeziel Dener ORCID: https://orcid.org/0000-0003-2077-3214, Marques, Catarina A.
ORCID: https://orcid.org/0000-0003-1324-5448, Beraldi, Dario, Crouch, Kathryn
ORCID: https://orcid.org/0000-0001-9310-4762, Lapsley, Craig, Obonaga, Ricardo, Tosi, Luiz R.O. and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2020)
Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication.
eLife, 9,
e58030.
(doi: 10.7554/eLife.58030)
(PMID:32897188)
(PMCID:PMC7511235)
Damasceno, Jeziel D. ORCID: https://orcid.org/0000-0003-2077-3214, Reis-Cunha, João, Crouch, Kathryn
ORCID: https://orcid.org/0000-0001-9310-4762, Beraldi, Dario, Lapsley, Craig, Tosi, Luiz R.O., Bartholomeu, Daniella and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2020)
Conditional knockout of RAD51-related genes in Leishmania major reveals a critical role for homologous recombination during genome replication.
PLoS Genetics, 16(7),
e1008828.
(doi: 10.1371/journal.pgen.1008828)
(PMID:32609721)
(PMCID:PMC7360064)
Black, Jennifer Ann, Crouch, Kathryn ORCID: https://orcid.org/0000-0001-9310-4762, Lemgruber, Leandro
ORCID: https://orcid.org/0000-0003-2832-6806, Lapsley, Craig, Dickens, Nicholas
ORCID: https://orcid.org/0000-0003-0492-5855, Tosi, Luiz R.O., Mottram, Jeremy C.
ORCID: https://orcid.org/0000-0001-5574-3766 and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2020)
Trypanosoma brucei ATR links DNA damage signalling during antigenic variation with regulation of RNA Polymerase I-transcribed surface antigens.
Cell Reports, 30(3),
836-851.e5.
(doi: 10.1016/j.celrep.2019.12.049)
(PMID:31968257)
(PMCID:PMC6988115)
2019
Briggs, Emma, Crouch, Kathryn ORCID: https://orcid.org/0000-0001-9310-4762, Lemgruber Soares, Leandro
ORCID: https://orcid.org/0000-0003-2832-6806, Hamilton, Graham, Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2019)
Trypanosoma brucei ribonuclease H2A is an essential R-loop processing enzyme whose loss causes DNA damage during transcription initiation and antigenic variation.
Nucleic Acids Research, 47(17),
pp. 9180-9197.
(doi: 10.1093/nar/gkz644)
(PMID:31350892)
(PMCID:PMC6753483)
2018
Briggs, Emma, Hamilton, Graham, Crouch, Kathryn ORCID: https://orcid.org/0000-0001-9310-4762, Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2018)
Genome-wide mapping reveals conserved and diverged R-loop activities in the unusual genetic landscape of the African trypanosome genome.
Nucleic Acids Research, 46(22),
pp. 11789-11805.
(doi: 10.1093/nar/gky928)
(PMID:30304482)
(PMCID:PMC6294496)
Briggs, Emma, Crouch, Kathryn ORCID: https://orcid.org/0000-0001-9310-4762, Lemgruber, Leandro
ORCID: https://orcid.org/0000-0003-2832-6806, Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2018)
Ribonuclease H1-targeted R-loops in surface antigen gene expression sites can direct trypanosome immune evasion.
PLoS Genetics, 14(12),
e1007729.
(doi: 10.1371/journal.pgen.1007729)
(PMID:30543624)
(PMCID:PMC6292569)
2016
Devlin, Rebecca, Marques, Catarina A. ORCID: https://orcid.org/0000-0003-1324-5448, Paape, Daniel
ORCID: https://orcid.org/0000-0003-4355-8058, Prorocic, Marko, Zurita-Leal, Andrea C., Campbell, Samantha J., Lapsley, Craig, Dickens, Nicholas
ORCID: https://orcid.org/0000-0003-0492-5855 and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2016)
Mapping replication dynamics in Trypanosoma brucei reveals a link with telomere transcription and antigenic variation.
eLife, 5,
e12765.
(doi: 10.7554/eLife.12765)
(PMID:27228154)
(PMCID:PMC4946898)
2011
Dobson, Rachel, Stockdale, Christopher, Lapsley, Craig, Wilkes, Jonathan ORCID: https://orcid.org/0000-0002-0920-6967 and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2011)
Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation.
Molecular Microbiology, 81(2),
pp. 434-456.
(doi: 10.1111/j.1365-2958.2011.07703.x)
Articles
Damasceno, Jeziel D. ORCID: https://orcid.org/0000-0003-2077-3214, Briggs, Emma M., Krasilnikova, Marija
ORCID: https://orcid.org/0000-0002-9140-9746, Marques, Catarina A., Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2025)
R-loops acted on by RNase H1 influence DNA replication timing and genome stability in Leishmania.
Nature Communications, 16,
1470.
(doi: 10.1038/s41467-025-56785-y)
(PMID:39922816)
(PMCID:PMC11807225)
Krasilnikova, Marija ORCID: https://orcid.org/0000-0002-9140-9746, Marques, Catarina A.
ORCID: https://orcid.org/0000-0003-1324-5448, Briggs, Emma, Lapsley, Craig, Hamilton, Graham, Beraldi, Dario, Crouch, Kathryn
ORCID: https://orcid.org/0000-0001-9310-4762 and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2025)
Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei.
Nature Communications, 16,
751.
(doi: 10.1038/s41467-025-56087-3)
Damasceno, Jeziel ORCID: https://orcid.org/0000-0003-2077-3214, LA. Silva, Gabriel, Marques, Catarina A.
ORCID: https://orcid.org/0000-0003-1324-5448, Krasilnikova, Marija
ORCID: https://orcid.org/0000-0002-9140-9746, Lapsley, Craig, Beraldi, Dario and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2024)
Nuclear DNA replication in Leishmania major relies on a single constitutive origin per chromosome supplemented by thousands of stochastic initiation events.
bioRxiv,
(doi: 10.1101/2024.11.14.623610)
Damasceno, Jeziel D. ORCID: https://orcid.org/0000-0003-2077-3214, Briggs, Emma M., Krasilnikova, Marija
ORCID: https://orcid.org/0000-0002-9140-9746, Marques, Catarina A.
ORCID: https://orcid.org/0000-0003-1324-5448, Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2024)
R-loops acted on by RNase H1 are a determinant of chromosome length-associated DNA replication timing and genome stability in Leishmania.
bioRxiv,
(doi: 10.1101/2024.04.29.591643)
Girasol, M. J. et al. (2023) RAD51-mediated R-loop formation acts to repair transcription associated DNA breaks driving antigenic variation in Trypanosoma brucei. Proceedings of the National Academy of Sciences of the United States of America, 120(48), e230930612. (doi: 10.1073/pnas.2309306120) (PMID:37988471) (PMCID:PMC10691351)
Damasceno, Jeziel Dener ORCID: https://orcid.org/0000-0003-2077-3214, Marques, Catarina A.
ORCID: https://orcid.org/0000-0003-1324-5448, Beraldi, Dario, Crouch, Kathryn
ORCID: https://orcid.org/0000-0001-9310-4762, Lapsley, Craig, Obonaga, Ricardo, Tosi, Luiz R.O. and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2020)
Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication.
eLife, 9,
e58030.
(doi: 10.7554/eLife.58030)
(PMID:32897188)
(PMCID:PMC7511235)
Damasceno, Jeziel D. ORCID: https://orcid.org/0000-0003-2077-3214, Reis-Cunha, João, Crouch, Kathryn
ORCID: https://orcid.org/0000-0001-9310-4762, Beraldi, Dario, Lapsley, Craig, Tosi, Luiz R.O., Bartholomeu, Daniella and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2020)
Conditional knockout of RAD51-related genes in Leishmania major reveals a critical role for homologous recombination during genome replication.
PLoS Genetics, 16(7),
e1008828.
(doi: 10.1371/journal.pgen.1008828)
(PMID:32609721)
(PMCID:PMC7360064)
Black, Jennifer Ann, Crouch, Kathryn ORCID: https://orcid.org/0000-0001-9310-4762, Lemgruber, Leandro
ORCID: https://orcid.org/0000-0003-2832-6806, Lapsley, Craig, Dickens, Nicholas
ORCID: https://orcid.org/0000-0003-0492-5855, Tosi, Luiz R.O., Mottram, Jeremy C.
ORCID: https://orcid.org/0000-0001-5574-3766 and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2020)
Trypanosoma brucei ATR links DNA damage signalling during antigenic variation with regulation of RNA Polymerase I-transcribed surface antigens.
Cell Reports, 30(3),
836-851.e5.
(doi: 10.1016/j.celrep.2019.12.049)
(PMID:31968257)
(PMCID:PMC6988115)
Briggs, Emma, Crouch, Kathryn ORCID: https://orcid.org/0000-0001-9310-4762, Lemgruber Soares, Leandro
ORCID: https://orcid.org/0000-0003-2832-6806, Hamilton, Graham, Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2019)
Trypanosoma brucei ribonuclease H2A is an essential R-loop processing enzyme whose loss causes DNA damage during transcription initiation and antigenic variation.
Nucleic Acids Research, 47(17),
pp. 9180-9197.
(doi: 10.1093/nar/gkz644)
(PMID:31350892)
(PMCID:PMC6753483)
Briggs, Emma, Hamilton, Graham, Crouch, Kathryn ORCID: https://orcid.org/0000-0001-9310-4762, Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2018)
Genome-wide mapping reveals conserved and diverged R-loop activities in the unusual genetic landscape of the African trypanosome genome.
Nucleic Acids Research, 46(22),
pp. 11789-11805.
(doi: 10.1093/nar/gky928)
(PMID:30304482)
(PMCID:PMC6294496)
Briggs, Emma, Crouch, Kathryn ORCID: https://orcid.org/0000-0001-9310-4762, Lemgruber, Leandro
ORCID: https://orcid.org/0000-0003-2832-6806, Lapsley, Craig and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2018)
Ribonuclease H1-targeted R-loops in surface antigen gene expression sites can direct trypanosome immune evasion.
PLoS Genetics, 14(12),
e1007729.
(doi: 10.1371/journal.pgen.1007729)
(PMID:30543624)
(PMCID:PMC6292569)
Devlin, Rebecca, Marques, Catarina A. ORCID: https://orcid.org/0000-0003-1324-5448, Paape, Daniel
ORCID: https://orcid.org/0000-0003-4355-8058, Prorocic, Marko, Zurita-Leal, Andrea C., Campbell, Samantha J., Lapsley, Craig, Dickens, Nicholas
ORCID: https://orcid.org/0000-0003-0492-5855 and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2016)
Mapping replication dynamics in Trypanosoma brucei reveals a link with telomere transcription and antigenic variation.
eLife, 5,
e12765.
(doi: 10.7554/eLife.12765)
(PMID:27228154)
(PMCID:PMC4946898)
Dobson, Rachel, Stockdale, Christopher, Lapsley, Craig, Wilkes, Jonathan ORCID: https://orcid.org/0000-0002-0920-6967 and McCulloch, Richard
ORCID: https://orcid.org/0000-0001-5739-976X
(2011)
Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation.
Molecular Microbiology, 81(2),
pp. 434-456.
(doi: 10.1111/j.1365-2958.2011.07703.x)