Dr Richard McCulloch

  • Reader (Parasitology)
  • Associate (School of Life Sciences)

Research interests

Current Research: DNA recombination, replication, repair and antigenic variation in trypanosomatid parasites

DNA recombination, replication and repair are central to the functioning and propagation of all organisms, maintaining genome stability for growth and reproduction. Homologous recombination, one of a number of repair pathways in cells, functions in repair of DNA double strand breaks, a severe form of genome damage, and ensures completion of DNA replication. In African trypanosomes, homologous recombination also plays a pivotal role in antigenic variation, a survival strategy that relies on changes in surface antigens (Variant Surface Glycoproteins), allowing host immune response evasion and infection of partially immune hosts. This is a hugely elaborate system, involving expression of a single VSG gene from >1000 silent copies in the genome. We are exploring how homologous recombination and other DNA repair pathways operate in trypanosomes and influence antigenic variation. In addition, we are characterising the process of nuclear DNA replication, which not only appears unusual in this parasite but, remarkably, also influences antigenic variation. Ongoing projects are examining homologous recombination in response to induced DNA double strand breaks, in characterising the machinery and timing of nuclear replication, examining the role of excision repair pathways in mitochondrial and nuclear genome stability, elucidating the DNA damage response in T. brucei, and in the enzymatic machinery and initiation of VSG switching throughout T. brucei chronic infections.


Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Does genome replication in the protozoan parasite Leishmania rely on origin-indepenent initiation?
    Biotechnology and Biological Sciences Research Council
    2018 - 2021
     
  • Challenging trypanosome antigenic variation paradigms using natural systems
    Wellcome Trust
    2018 - 2022
     
  • RECREPEMLE
    European Commission
    2017 - 2019
     
  • How do common and diverged features of the replicative stress response shape the biology of TriTryp
    Biotechnology and Biological Sciences Research Council
    2016 - 2019
     
  • 14CONFAP Understanding diverged genome repair and replication functions in trypanosomatid parasites
    Biotechnology and Biological Sciences Research Council
    2015 - 2016
     
  • The Wellcome Centre for Molecular Parasitology ( Core Support )
    Wellcome Trust
    2014 - 2021
     
  • Kinase dependent control of DNA replication and repair as a drug target in Trypanosoma brucei.
    Biotechnology and Biological Sciences Research Council
    2013 - 2016
     
  • Chromosomal recombination and repair in African trypanosomes
    Wellcome Trust
    2009 - 2014
     
  • Characterisation of Orc1/Cdc6 and DNA replication initiation in Trypanosoma brucei.
    Wellcome Trust
    2008 - 2011
     
  • DNA recombination pathways and antigenic variation in trypanosoma brucei
    Medical Research Council
    2006 - 2008
     
  • DNA recombination in trypanosome antigenic variation
    The Royal Society
    1997 - 2006
     

Additional information

Invited International Presentations

  • 2013: Foz do Iguacu, Brazil - 11th International Conference on Environmental Mutagens
  • 2013: Woods Hole, Massachusetts, USA - Plenary lecture, V Kinetoplastid Molecular Cell Biology meeting
  • 2013: Sao Paulo, Brazil - V Fundamental Aspects of DNA repair and Mutagenesis symposium
  • 2012: Glasgow, Scotland, UK - British Society for Parasitology Spring meeting and Trypanosomiasis and Leishmaniasis symposium
  • 2011: Foz do Iguacu, Brazil - XXVI Annual Meeting of the Brazilian Society of Protozoology
  • 2011: Woods Hole, Massachusetts, USA - IV Kinetoplastid Molecular Cell Biology meeting
  • 2010: Annecy, France - Fondation Merieux sponsored conference on antigenic variation and immune evasion
  • 2009: Belo Horizonte, Brazil - IV Fundamental Aspects of DNA Repair and Mutagenesis symposium
  • 2008: Newcastle, England, UK - British Society of Parasitology Spring meeting and Trypanosomiasis and Leishmaniasis symposium
  • 2007: Belo Horizonte, Brazil - Universidade Federal de Minas Gerais
  • 2007: Rio de Janeiro, Brazil - VIII Brazilian Environmental Mutagenesis, Carcinogenesis and Teratogenesis Meeting
  • 2007: Caxambu, Brazil - XXII Annual Meeting of the Brazilian Society of Protozoology
  • 2006: Glasgow, Scotland, UK - ICOPA IX (11th International Conference of Parasitology)
  • 2006: Orlando, Florida, USA - Divisional Lecture, American Society for Microbilogy 106th General Meeting
  • 2005: Keystone, Colorado, USA - Keystone symposium on DNA replication and recombination
  • 2005: Woods Hole, Massachusetts, USA - Kinetoplastid Molecular and Cell Biology meeting
  • 2004: Cambridge, England, UK - Trypanosomatid genome sequence annotation meeting, Wellcome Sanger Centre
  • 2004: Ceske Budejovice, Czech Republic - British Society for Parasitology Trypanosomiasis and Leishmaniasis Seminar
  • 2003: Woods Hole, Massachusetts, USA - Molecular Parasitology meeting
  • 2002: Snowbird, Utah, USA - Keystone symposium on DNA replication and recombination
  • 2002: Woods Hole, Massachusetts, USA - Molecular Parasitology meeting

Research Fellowship

  • 1997 - 2006: Royal Society University Research Fellowship
  • 1994 - 1997: Wellcome Trust International Prize Travelling Fellowship

Publications

List by: Type | Date

Jump to: 2019 | 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001
Number of items: 53.

2019

Jayaraman, S. et al. (2019) Application of long read sequencing to determine expressed antigen diversity in Trypanosoma brucei infections. PLoS Neglected Tropical Diseases, (doi:10.1371/journal.pntd.0007262) (PMID:30943202) (Early Online Publication)

Vieira Da Rocha, J.P., Passos-Silva, D.G., Mendes, I.C., Rocha, E.A., Gomes, D.A., Machado, C.R. and McCulloch, R. (2019) The DNA damage response is developmentally regulated in the African trypanosome. DNA Repair, 73, pp. 78-90. (doi:10.1016/j.dnarep.2018.11.005) (PMID:30470509) (PMCID:PMC6329875)

2018

Briggs, E., Hamilton, G., Crouch, K. , Lapsley, C. and McCulloch, R. (2018) Genome-wide mapping reveals conserved and diverged R-loop activities in the unusual genetic landscape of the African trypanosome genome. Nucleic Acids Research, 46(22), pp. 11789-11805. (doi:10.1093/nar/gky928) (PMID:30304482) (PMCID:PMC6294496)

Briggs, E., Crouch, K. , Lemgruber, L., Lapsley, C. and McCulloch, R. (2018) Ribonuclease H1-targeted R-loops in surface antigen gene expression sites can direct trypanosome immune evasion. PLoS Genetics, 14(12), e1007729. (doi:10.1371/journal.pgen.1007729) (PMID:30543624) (PMCID:PMC6292569)

Santos da Silva, M., Hovel-Miner, G. A., Briggs, E. M., Elias, M. C. and McCulloch, R. (2018) Evaluation of mechanisms that may generate DNA lesions triggering antigenic variation in African trypanosomes. PLoS Pathogens, 14(11), e1007321. (doi:10.1371/journal.ppat.1007321) (PMID:30440029) (PMCID:PMC6237402)

Gomes Passos Silva, D. et al. (2018) The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions. PLoS Neglected Tropical Diseases, 12(11), e0006875. (doi:10.1371/journal.pntd.0006875) (PMID:30422982) (PMCID:PMC6258567)

Damasceno, J. D., Obonaga, R., Silva, G. L.A., Reis-Cunha, J. L., Duncan, S. M., Bartholomeu, D. C., Mottram, J. C. , McCulloch, R. and Tosi, L. R.O. (2018) Conditional genome engineering reveals canonical and divergent roles for the Hus1 component of the 9-1-1 complex in the maintenance of the plastic genome of Leishmania. Nucleic Acids Research, (doi:10.1093/nar/gky1017) (PMID:30380080) (Early Online Publication)

Marques, C. A. and McCulloch, R. (2018) Conservation and variation in strategies for DNA replication of kinetoplastid nuclear genomes. Current Genomics, 19(2), pp. 98-109. (doi:10.2174/1389202918666170815144627) (PMID:29491738) (PMCID:PMC5814967)

2017

McCulloch, R. , Cobbold, C. A., Figueiredo, L., Jackson, A., Morrison, L. J., Mugnier, M. R., Papavasiliou, N., Schnaufer, A. and Matthews, K. (2017) Emerging challenges in understanding trypanosome antigenic variation. Emerging Topics in Life Sciences, 1(6), pp. 585-592. (doi:10.1042/ETLS20170104)

da Silva, M. S., Pavani, R. S., Damasceno, J. D., Marques, C. A., McCulloch, R. , Tosi, L. R. O. and Elias, M. C. (2017) Nuclear DNA replication in trypanosomatids: there are no easy methods for solving difficult problems. Trends in Parasitology, 33(11), pp. 858-874. (doi:10.1016/j.pt.2017.08.002) (PMID:28844718) (PMCID:PMC5662062)

Stortz, J. A. et al. (2017) Genome-wide and protein kinase-focused RNAi screens reveal conserved and novel damage response pathways in Trypanosoma brucei. PLoS Pathogens, 13(7), e1006477. (doi:10.1371/journal.ppat.1006477) (PMID:28742144) (PMCID:PMC5542689)

Fernandez-Cortes, F., Serafim, T. D., Wilkes, J. M. , Jones, N. G., Ritchie, R., McCulloch, R. and Mottram, J. C. (2017) RNAi screening identifies Trypanosoma brucei stress response protein kinases required for survival in the mouse. Scientific Reports, 7, 6156. (doi:10.1038/s41598-017-06501-8) (PMID:28733613) (PMCID:PMC5522463)

Devlin, R., Marques, C. A. and McCulloch, R. (2017) Does DNA replication direct locus-specific recombination during host immune evasion by antigenic variation in the African trypanosome? Current Genetics, 63(3), pp. 441-449. (doi:10.1007/s00294-016-0662-7) (PMID:27822899) (PMCID:PMC5422504)

Cabrera, G., Silva, V. G., Mendes, I. C., Machado, C. R. and McCulloch, R. (2017) Genome maintenance in Trypanosomatids. In: Santos da Silva, M. and Cano, M. I. N. (eds.) Molecular and Cellular Biology of Pathogenic Trypanosomatids. Series: Frontiers in parasitology, 1. Bentham Science, pp. 195-260. ISBN 9781681084060 (doi:10.2174/9781681084053117010008)

2016

Biéler, S. et al. (2016) Evaluation of antigens for development of a serological test for human African trypanosomiasis. PLoS ONE, 11(12), e0168074. (doi:10.1371/journal.pone.0168074) (PMID:27936225) (PMCID:PMC5148118)

Marques, C. A., Tiengwe, C., Lemgruber Soares, L., Damasceno, J. D., Scott, A., Paape, D. , Marcello, L. and McCulloch, R. (2016) Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation. Nucleic Acids Research, 44(10), pp. 4763-4784. (doi:10.1093/nar/gkw147) (PMID:26951375) (PMCID:PMC4889932)

Damasceno, J. D., Obonaga, R., Santos, E. V., Scott, A., McCulloch, R. and Tosi, L. R.O. (2016) Functional compartmentalization of Rad9 and Hus1 reveals diverse assembly of the 9-1-1 complex components during the DNA damage response in Leishmania. Molecular Microbiology, 101(6), pp. 1054-1068. (doi:10.1111/mmi.13441) (PMID:27301589) (PMCID:PMC5453112)

McCulloch, R. and Navarro, M. (2016) The protozoan nucleus. Molecular and Biochemical Parasitology, 209(1-2), pp. 76-87. (doi:10.1016/j.molbiopara.2016.05.002) (PMID:27181562)

Devlin, R., Marques, C. A. , Paape, D. , Prorocic, M., Zurita-Leal, A. C., Campbell, S. J., Lapsley, C., Dickens, N. and McCulloch, R. (2016) Mapping replication dynamics in Trypanosoma brucei reveals a link with telomere transcription and antigenic variation. eLife, 5, e12765. (doi:10.7554/eLife.12765) (PMID:27228154) (PMCID:PMC4946898)

2015

Rodenko, B., Wanner, M., Alkhaldi, A., Ebiloma, G., Barnes, R., Kaiser, M., Brun, R., McCulloch, R. , Koomen, G.-J. and De Koning, H. P. (2015) Targeting the parasite's DNA with methyltriazenyl purine analogs Is a safe, selective, and efficacious antitrypanosomal strategy. Antimicrobial Agents and Chemotherapy, 59(11), pp. 6708-6716. (doi:10.1128/aac.00596-15) (PMID:26282430) (PMCID:PMC4604408)

McCulloch, R. and Field, M. C. (2015) Quantitative sequencing confirms VSG diversity as central to immune evasion by Trypanosoma brucei. Trends in Parasitology, 31(8), pp. 346-349. (doi:10.1016/j.pt.2015.05.001) (PMID:25999027)

Calderano, S.G., Drosopoulos, W.C., Quaresma, M. M., Marques, C.A., Kosiyatrakul, S., McCulloch, R. , Schildkraut, C.L. and Elias, M.C. (2015) Single molecule analysis of Trypanosoma brucei DNA replication dynamics. Nucleic Acids Research, 43(5), pp. 2655-2665. (doi:10.1093/nar/gku1389) (PMID:25690894) (PMCID:PMC4357695)

Grazielle-Silva, V., Zeb, T.F., Bolderson, J., Campos, P.C., Miranda, J.B., Alves, C.L., Machado, C.R., McCulloch, R. and Teixeira, S.M. R. (2015) Distinct phenotypes caused by mutation of MSH2 in trypanosome insect and mammalian life cycle forms are associated with parasite adaptation to oxidative stress. PLoS Neglected Tropical Diseases, 9(6), e0003870. (doi:10.1371/journal.pntd.0003870) (PMID:26083967) (PMCID:PMC4470938)

Marques, C. A., Dickens, N. J. , Paape, D. , Campbell, S. J. and McCulloch, R. (2015) Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe. Genome Biology, 16, 230. (doi:10.1186/s13059-015-0788-9) (PMID:26481451) (PMCID:PMC4612428)

2014

Machado, C. R. et al. (2014) Nucleotide excision repair in Trypanosoma brucei: specialization of transcription-coupled repair due to multigenic transcription. Molecular Microbiology, 92(4), pp. 756-776. (doi:10.1111/mmi.12589)

Tiengwe, C., Marques, C. A. and McCulloch, R. (2014) Nuclear DNA replication initiation in kinetoplastid parasites: new insights into an ancient process. Trends in Parasitology, 30(1), pp. 27-36. (doi:10.1016/j.pt.2013.10.009)

2013

Trenaman, A., Hartley, C., Prorocic, M., Passos-Silva, D. G., van den Hoek, M., Nechyporuk-Zloy, V., Machado, C. R. and McCulloch, R. (2013) Trypanosoma brucei BRCA2 acts in a life cycle-specific genome stability process and dictates BRC repeat number-dependent RAD51 subnuclear dynamics. Nucleic Acids Research, 41(2), pp. 943-960. (doi:10.1093/nar/gks1192)

2012

Tiengwe, C. et al. (2012) Genome-wide analysis reveals extensive functional interaction between DNA replication initiation and transcription in the genome of trypanosoma brucei. Cell Reports, 2(1), pp. 185-197. (doi:10.1016/j.celrep.2012.06.007)

Tiengwe, C., Marcello, L., Farr, H., Gadelha, C., Burchmore, R. , Barry, J.D., Bell, S.D. and McCulloch, R. (2012) Identification of ORC1/CDC6-interacting factors in trypanosoma brucei reveals critical features of origin recognition complex architecture. PLoS ONE, 7(3), e32674. (doi:10.1371/journal.pone.0032674) (PMID:22412905) (PMCID:PMC3297607)

2011

Dobson, R., Stockdale, C., Lapsley, C., Wilkes, J. and McCulloch, R. (2011) Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation. Molecular Microbiology, 81(2), pp. 434-456. (doi:10.1111/j.1365-2958.2011.07703.x)

2010

Horn, D. and McCulloch, R. (2010) Molecular mechanisms underlying the control of antigenic variation in African trypanosomes. Current Opinion in Microbiology, 13(6), pp. 700-705. (doi:10.1016/j.mib.2010.08.009)

2009

Morrison, L. J., Marcello, L. and McCulloch, R. (2009) Antigenic variation in the African trypanosome: molecular mechanisms and phenotypic complexity. Cellular Microbiology, 11(12), pp. 1724-1734. (doi:10.1111/j.1462-5822.2009.01383.x)

McCulloch, R. and Horn, D. (2009) What has DNA sequencing revealed about the VSG expression sites of African trypanosomes? Trends in Parasitology, 25(8), pp. 359-363. (doi:10.1016/j.pt.2009.05.007)

2008

Stockdale, C., Swiderski, M. R., Barry, J. D. and McCulloch, R. (2008) Antigenic variation in Trypanosoma brucei: joining the DOTs. PLoS Biology, 6(7), e185. (doi:10.1371/journal.pbio.0060185)

Hartley, C. L. and McCulloch, R. (2008) Trypanosoma brucei BRCA2 acts in antigenic variation and has undergone a recent expansion in BRC repeat number that is important during homologous recombination. Molecular Microbiology, 68(5), pp. 1237-1251. (doi:10.1111/j.1365-2958.2008.06230.x)

Glover, L., McCulloch, R. and Horn, D. (2008) Sequence homology and microhomology dominate chromosomal double-strand break repair in African trypanosomes. Nucleic Acids Research, 36(8), pp. 2608-2618. (doi:10.1093/nar/gkn104)

Machado-Silva, A., Teixeira, S. M.R., Franco, G. R., Macedo, A. M., Pena, S. D.J., McCulloch, R. and Machado, C. R. (2008) Mismatch repair in Trypanosoma brucei: Heterologous expression of MSH2 from Trypanosoma cruzi provides new insights into the response to oxidative damage. Gene, 411(1-2), pp. 19-26. (doi:10.1016/j.gene.2007.12.021)

2007

Burton, P., McBride, D. J., Wilkes, J. M., Barry, J. D. and McCulloch, R. (2007) Ku heterodimer-independent end joining in Trypanosoma brucei cell extracts relies upon sequence microhomology. Eukaryotic Cell, 6(10), pp. 1773-1781. (doi:10.1128/EC.00212-07)

Barnes, R. L. and McCulloch, R. (2007) Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases. Nucleic Acids Research, 35(10), pp. 3478-3493. (doi:10.1093/nar/gkm249)

2006

Machado, C.R., Augusto-Pinto, L., McCulloch, R. and Teixeira, S.M.R. (2006) DNA metabolism and genetic diversity in Trypanosomes. Mutation Research-Reviews in Mutation Research, 612, pp. 40-57. (doi:10.1016/j.mrrev.2005.05.001)

Proudfoot, C. and McCulloch, R. (2006) Trypanosoma brucei DMC1 does not act in DNA recombination, repair or antigenic variation in bloodstream stage cells. Molecular and Biochemical Parasitology, 145, pp. 245-253. (doi:10.1016/j.molbiopara.2005.10.007)

2005

El-Sayed, N.M. et al. (2005) The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science, 309(5733), pp. 409-415. (doi:10.1126/science.1112631)

Proudfoot, C. and McCulloch, R. (2005) Distinct roles for two RAD51-related genes in Trypanosoma brucei antigenic variation. Nucleic Acids Research, 33, pp. 6906-6919. (doi:10.1093/nar/gki)

2004

Bell, J., Harvey, T., Sims, A. and McCulloch, R. (2004) Characterization of components of the mismatch repair machinery in Trypanosoma brucei. Molecular Microbiology, 51, pp. 159-173. (doi:10.1046/j.1365-2958.2003.03804.x)

McCulloch, R. (2004) Antigenic variation in African trypanosomes: monitoring progress. Trends in Parasitology, 20, pp. 117-121.

McCulloch, R., Vassella, E., Burton, P., Boshart, M. and Barry, J.D. (2004) Transformation of monomorphic and pleomorphic Trypanosoma brucei. Methods in Molecular Biology, 262(1), pp. 53-86. (doi:10.1385/1592597610)

2003

Barry, J., Ginger, M., Burton, P. and McCulloch, R. (2003) Why are parasite contingency genes often associated with telomeres? International Journal for Parasitology, 33, pp. 29-45. (doi:10.1016/S0020-7519(02)00247-3)

Bell, J. and McCulloch, R. (2003) Mismatch repair regulates homologous recombination, but has little influence on antigenic variation, in Trypanosoma brucei. Journal of Biological Chemistry, 278, pp. 45182-45188. (doi:10.1074/jbc.M308123200)

2002

Conway, C., McCulloch, R., Ginger, M., Robinson, N., Browitt, A. and Barry, J. (2002) Ku is important for telomere maintenance, but not for differential expression of telomeric VSG genes, in African trypanosomes. Journal of Biological Chemistry, 277, pp. 21269-21277. (doi:10.1074/jbc.M200550200)

Conway, C., Proudfoot, C., Burton, P., Barry, J. and McCulloch, R. (2002) Two pathways of homologous recombination in Trypanosoma brucei. Molecular Microbiology, 45, pp. 1687-1700.

Robinson, N., McCulloch, R., Conway, C., Browitt, A. and Barry, J. (2002) Inactivation of Mre11 does not affect VSG gene duplication mediated by homologous recombination in Trypanosoma brucei. Journal of Biological Chemistry, 277, pp. 26185-26193. (doi:10.1074/jbc.M203205200)

2001

Barry, J. and McCulloch, R. (2001) Antigenic variation in trypanosomes: Enhanced phenotypic variation in a eukaryotic parasite. Advances in Parasitology, 49, pp. 1-70.

Vanhamme, L., Pays, E., McCulloch, R. and Barry, J. (2001) An update on antigenic variation in African trypanosomes. Trends in Parasitology, 17, pp. 338-343.

This list was generated on Sat May 25 13:25:50 2019 BST.
Number of items: 53.

Articles

Jayaraman, S. et al. (2019) Application of long read sequencing to determine expressed antigen diversity in Trypanosoma brucei infections. PLoS Neglected Tropical Diseases, (doi:10.1371/journal.pntd.0007262) (PMID:30943202) (Early Online Publication)

Vieira Da Rocha, J.P., Passos-Silva, D.G., Mendes, I.C., Rocha, E.A., Gomes, D.A., Machado, C.R. and McCulloch, R. (2019) The DNA damage response is developmentally regulated in the African trypanosome. DNA Repair, 73, pp. 78-90. (doi:10.1016/j.dnarep.2018.11.005) (PMID:30470509) (PMCID:PMC6329875)

Briggs, E., Hamilton, G., Crouch, K. , Lapsley, C. and McCulloch, R. (2018) Genome-wide mapping reveals conserved and diverged R-loop activities in the unusual genetic landscape of the African trypanosome genome. Nucleic Acids Research, 46(22), pp. 11789-11805. (doi:10.1093/nar/gky928) (PMID:30304482) (PMCID:PMC6294496)

Briggs, E., Crouch, K. , Lemgruber, L., Lapsley, C. and McCulloch, R. (2018) Ribonuclease H1-targeted R-loops in surface antigen gene expression sites can direct trypanosome immune evasion. PLoS Genetics, 14(12), e1007729. (doi:10.1371/journal.pgen.1007729) (PMID:30543624) (PMCID:PMC6292569)

Santos da Silva, M., Hovel-Miner, G. A., Briggs, E. M., Elias, M. C. and McCulloch, R. (2018) Evaluation of mechanisms that may generate DNA lesions triggering antigenic variation in African trypanosomes. PLoS Pathogens, 14(11), e1007321. (doi:10.1371/journal.ppat.1007321) (PMID:30440029) (PMCID:PMC6237402)

Gomes Passos Silva, D. et al. (2018) The in vivo and in vitro roles of Trypanosoma cruzi Rad51 in the repair of DNA double strand breaks and oxidative lesions. PLoS Neglected Tropical Diseases, 12(11), e0006875. (doi:10.1371/journal.pntd.0006875) (PMID:30422982) (PMCID:PMC6258567)

Damasceno, J. D., Obonaga, R., Silva, G. L.A., Reis-Cunha, J. L., Duncan, S. M., Bartholomeu, D. C., Mottram, J. C. , McCulloch, R. and Tosi, L. R.O. (2018) Conditional genome engineering reveals canonical and divergent roles for the Hus1 component of the 9-1-1 complex in the maintenance of the plastic genome of Leishmania. Nucleic Acids Research, (doi:10.1093/nar/gky1017) (PMID:30380080) (Early Online Publication)

Marques, C. A. and McCulloch, R. (2018) Conservation and variation in strategies for DNA replication of kinetoplastid nuclear genomes. Current Genomics, 19(2), pp. 98-109. (doi:10.2174/1389202918666170815144627) (PMID:29491738) (PMCID:PMC5814967)

McCulloch, R. , Cobbold, C. A., Figueiredo, L., Jackson, A., Morrison, L. J., Mugnier, M. R., Papavasiliou, N., Schnaufer, A. and Matthews, K. (2017) Emerging challenges in understanding trypanosome antigenic variation. Emerging Topics in Life Sciences, 1(6), pp. 585-592. (doi:10.1042/ETLS20170104)

da Silva, M. S., Pavani, R. S., Damasceno, J. D., Marques, C. A., McCulloch, R. , Tosi, L. R. O. and Elias, M. C. (2017) Nuclear DNA replication in trypanosomatids: there are no easy methods for solving difficult problems. Trends in Parasitology, 33(11), pp. 858-874. (doi:10.1016/j.pt.2017.08.002) (PMID:28844718) (PMCID:PMC5662062)

Stortz, J. A. et al. (2017) Genome-wide and protein kinase-focused RNAi screens reveal conserved and novel damage response pathways in Trypanosoma brucei. PLoS Pathogens, 13(7), e1006477. (doi:10.1371/journal.ppat.1006477) (PMID:28742144) (PMCID:PMC5542689)

Fernandez-Cortes, F., Serafim, T. D., Wilkes, J. M. , Jones, N. G., Ritchie, R., McCulloch, R. and Mottram, J. C. (2017) RNAi screening identifies Trypanosoma brucei stress response protein kinases required for survival in the mouse. Scientific Reports, 7, 6156. (doi:10.1038/s41598-017-06501-8) (PMID:28733613) (PMCID:PMC5522463)

Devlin, R., Marques, C. A. and McCulloch, R. (2017) Does DNA replication direct locus-specific recombination during host immune evasion by antigenic variation in the African trypanosome? Current Genetics, 63(3), pp. 441-449. (doi:10.1007/s00294-016-0662-7) (PMID:27822899) (PMCID:PMC5422504)

Biéler, S. et al. (2016) Evaluation of antigens for development of a serological test for human African trypanosomiasis. PLoS ONE, 11(12), e0168074. (doi:10.1371/journal.pone.0168074) (PMID:27936225) (PMCID:PMC5148118)

Marques, C. A., Tiengwe, C., Lemgruber Soares, L., Damasceno, J. D., Scott, A., Paape, D. , Marcello, L. and McCulloch, R. (2016) Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation. Nucleic Acids Research, 44(10), pp. 4763-4784. (doi:10.1093/nar/gkw147) (PMID:26951375) (PMCID:PMC4889932)

Damasceno, J. D., Obonaga, R., Santos, E. V., Scott, A., McCulloch, R. and Tosi, L. R.O. (2016) Functional compartmentalization of Rad9 and Hus1 reveals diverse assembly of the 9-1-1 complex components during the DNA damage response in Leishmania. Molecular Microbiology, 101(6), pp. 1054-1068. (doi:10.1111/mmi.13441) (PMID:27301589) (PMCID:PMC5453112)

McCulloch, R. and Navarro, M. (2016) The protozoan nucleus. Molecular and Biochemical Parasitology, 209(1-2), pp. 76-87. (doi:10.1016/j.molbiopara.2016.05.002) (PMID:27181562)

Devlin, R., Marques, C. A. , Paape, D. , Prorocic, M., Zurita-Leal, A. C., Campbell, S. J., Lapsley, C., Dickens, N. and McCulloch, R. (2016) Mapping replication dynamics in Trypanosoma brucei reveals a link with telomere transcription and antigenic variation. eLife, 5, e12765. (doi:10.7554/eLife.12765) (PMID:27228154) (PMCID:PMC4946898)

Rodenko, B., Wanner, M., Alkhaldi, A., Ebiloma, G., Barnes, R., Kaiser, M., Brun, R., McCulloch, R. , Koomen, G.-J. and De Koning, H. P. (2015) Targeting the parasite's DNA with methyltriazenyl purine analogs Is a safe, selective, and efficacious antitrypanosomal strategy. Antimicrobial Agents and Chemotherapy, 59(11), pp. 6708-6716. (doi:10.1128/aac.00596-15) (PMID:26282430) (PMCID:PMC4604408)

McCulloch, R. and Field, M. C. (2015) Quantitative sequencing confirms VSG diversity as central to immune evasion by Trypanosoma brucei. Trends in Parasitology, 31(8), pp. 346-349. (doi:10.1016/j.pt.2015.05.001) (PMID:25999027)

Calderano, S.G., Drosopoulos, W.C., Quaresma, M. M., Marques, C.A., Kosiyatrakul, S., McCulloch, R. , Schildkraut, C.L. and Elias, M.C. (2015) Single molecule analysis of Trypanosoma brucei DNA replication dynamics. Nucleic Acids Research, 43(5), pp. 2655-2665. (doi:10.1093/nar/gku1389) (PMID:25690894) (PMCID:PMC4357695)

Grazielle-Silva, V., Zeb, T.F., Bolderson, J., Campos, P.C., Miranda, J.B., Alves, C.L., Machado, C.R., McCulloch, R. and Teixeira, S.M. R. (2015) Distinct phenotypes caused by mutation of MSH2 in trypanosome insect and mammalian life cycle forms are associated with parasite adaptation to oxidative stress. PLoS Neglected Tropical Diseases, 9(6), e0003870. (doi:10.1371/journal.pntd.0003870) (PMID:26083967) (PMCID:PMC4470938)

Marques, C. A., Dickens, N. J. , Paape, D. , Campbell, S. J. and McCulloch, R. (2015) Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe. Genome Biology, 16, 230. (doi:10.1186/s13059-015-0788-9) (PMID:26481451) (PMCID:PMC4612428)

Machado, C. R. et al. (2014) Nucleotide excision repair in Trypanosoma brucei: specialization of transcription-coupled repair due to multigenic transcription. Molecular Microbiology, 92(4), pp. 756-776. (doi:10.1111/mmi.12589)

Tiengwe, C., Marques, C. A. and McCulloch, R. (2014) Nuclear DNA replication initiation in kinetoplastid parasites: new insights into an ancient process. Trends in Parasitology, 30(1), pp. 27-36. (doi:10.1016/j.pt.2013.10.009)

Trenaman, A., Hartley, C., Prorocic, M., Passos-Silva, D. G., van den Hoek, M., Nechyporuk-Zloy, V., Machado, C. R. and McCulloch, R. (2013) Trypanosoma brucei BRCA2 acts in a life cycle-specific genome stability process and dictates BRC repeat number-dependent RAD51 subnuclear dynamics. Nucleic Acids Research, 41(2), pp. 943-960. (doi:10.1093/nar/gks1192)

Tiengwe, C. et al. (2012) Genome-wide analysis reveals extensive functional interaction between DNA replication initiation and transcription in the genome of trypanosoma brucei. Cell Reports, 2(1), pp. 185-197. (doi:10.1016/j.celrep.2012.06.007)

Tiengwe, C., Marcello, L., Farr, H., Gadelha, C., Burchmore, R. , Barry, J.D., Bell, S.D. and McCulloch, R. (2012) Identification of ORC1/CDC6-interacting factors in trypanosoma brucei reveals critical features of origin recognition complex architecture. PLoS ONE, 7(3), e32674. (doi:10.1371/journal.pone.0032674) (PMID:22412905) (PMCID:PMC3297607)

Dobson, R., Stockdale, C., Lapsley, C., Wilkes, J. and McCulloch, R. (2011) Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation. Molecular Microbiology, 81(2), pp. 434-456. (doi:10.1111/j.1365-2958.2011.07703.x)

Horn, D. and McCulloch, R. (2010) Molecular mechanisms underlying the control of antigenic variation in African trypanosomes. Current Opinion in Microbiology, 13(6), pp. 700-705. (doi:10.1016/j.mib.2010.08.009)

Morrison, L. J., Marcello, L. and McCulloch, R. (2009) Antigenic variation in the African trypanosome: molecular mechanisms and phenotypic complexity. Cellular Microbiology, 11(12), pp. 1724-1734. (doi:10.1111/j.1462-5822.2009.01383.x)

McCulloch, R. and Horn, D. (2009) What has DNA sequencing revealed about the VSG expression sites of African trypanosomes? Trends in Parasitology, 25(8), pp. 359-363. (doi:10.1016/j.pt.2009.05.007)

Stockdale, C., Swiderski, M. R., Barry, J. D. and McCulloch, R. (2008) Antigenic variation in Trypanosoma brucei: joining the DOTs. PLoS Biology, 6(7), e185. (doi:10.1371/journal.pbio.0060185)

Hartley, C. L. and McCulloch, R. (2008) Trypanosoma brucei BRCA2 acts in antigenic variation and has undergone a recent expansion in BRC repeat number that is important during homologous recombination. Molecular Microbiology, 68(5), pp. 1237-1251. (doi:10.1111/j.1365-2958.2008.06230.x)

Glover, L., McCulloch, R. and Horn, D. (2008) Sequence homology and microhomology dominate chromosomal double-strand break repair in African trypanosomes. Nucleic Acids Research, 36(8), pp. 2608-2618. (doi:10.1093/nar/gkn104)

Machado-Silva, A., Teixeira, S. M.R., Franco, G. R., Macedo, A. M., Pena, S. D.J., McCulloch, R. and Machado, C. R. (2008) Mismatch repair in Trypanosoma brucei: Heterologous expression of MSH2 from Trypanosoma cruzi provides new insights into the response to oxidative damage. Gene, 411(1-2), pp. 19-26. (doi:10.1016/j.gene.2007.12.021)

Burton, P., McBride, D. J., Wilkes, J. M., Barry, J. D. and McCulloch, R. (2007) Ku heterodimer-independent end joining in Trypanosoma brucei cell extracts relies upon sequence microhomology. Eukaryotic Cell, 6(10), pp. 1773-1781. (doi:10.1128/EC.00212-07)

Barnes, R. L. and McCulloch, R. (2007) Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases. Nucleic Acids Research, 35(10), pp. 3478-3493. (doi:10.1093/nar/gkm249)

Machado, C.R., Augusto-Pinto, L., McCulloch, R. and Teixeira, S.M.R. (2006) DNA metabolism and genetic diversity in Trypanosomes. Mutation Research-Reviews in Mutation Research, 612, pp. 40-57. (doi:10.1016/j.mrrev.2005.05.001)

Proudfoot, C. and McCulloch, R. (2006) Trypanosoma brucei DMC1 does not act in DNA recombination, repair or antigenic variation in bloodstream stage cells. Molecular and Biochemical Parasitology, 145, pp. 245-253. (doi:10.1016/j.molbiopara.2005.10.007)

El-Sayed, N.M. et al. (2005) The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science, 309(5733), pp. 409-415. (doi:10.1126/science.1112631)

Proudfoot, C. and McCulloch, R. (2005) Distinct roles for two RAD51-related genes in Trypanosoma brucei antigenic variation. Nucleic Acids Research, 33, pp. 6906-6919. (doi:10.1093/nar/gki)

Bell, J., Harvey, T., Sims, A. and McCulloch, R. (2004) Characterization of components of the mismatch repair machinery in Trypanosoma brucei. Molecular Microbiology, 51, pp. 159-173. (doi:10.1046/j.1365-2958.2003.03804.x)

McCulloch, R. (2004) Antigenic variation in African trypanosomes: monitoring progress. Trends in Parasitology, 20, pp. 117-121.

McCulloch, R., Vassella, E., Burton, P., Boshart, M. and Barry, J.D. (2004) Transformation of monomorphic and pleomorphic Trypanosoma brucei. Methods in Molecular Biology, 262(1), pp. 53-86. (doi:10.1385/1592597610)

Barry, J., Ginger, M., Burton, P. and McCulloch, R. (2003) Why are parasite contingency genes often associated with telomeres? International Journal for Parasitology, 33, pp. 29-45. (doi:10.1016/S0020-7519(02)00247-3)

Bell, J. and McCulloch, R. (2003) Mismatch repair regulates homologous recombination, but has little influence on antigenic variation, in Trypanosoma brucei. Journal of Biological Chemistry, 278, pp. 45182-45188. (doi:10.1074/jbc.M308123200)

Conway, C., McCulloch, R., Ginger, M., Robinson, N., Browitt, A. and Barry, J. (2002) Ku is important for telomere maintenance, but not for differential expression of telomeric VSG genes, in African trypanosomes. Journal of Biological Chemistry, 277, pp. 21269-21277. (doi:10.1074/jbc.M200550200)

Conway, C., Proudfoot, C., Burton, P., Barry, J. and McCulloch, R. (2002) Two pathways of homologous recombination in Trypanosoma brucei. Molecular Microbiology, 45, pp. 1687-1700.

Robinson, N., McCulloch, R., Conway, C., Browitt, A. and Barry, J. (2002) Inactivation of Mre11 does not affect VSG gene duplication mediated by homologous recombination in Trypanosoma brucei. Journal of Biological Chemistry, 277, pp. 26185-26193. (doi:10.1074/jbc.M203205200)

Barry, J. and McCulloch, R. (2001) Antigenic variation in trypanosomes: Enhanced phenotypic variation in a eukaryotic parasite. Advances in Parasitology, 49, pp. 1-70.

Vanhamme, L., Pays, E., McCulloch, R. and Barry, J. (2001) An update on antigenic variation in African trypanosomes. Trends in Parasitology, 17, pp. 338-343.

Book Sections

Cabrera, G., Silva, V. G., Mendes, I. C., Machado, C. R. and McCulloch, R. (2017) Genome maintenance in Trypanosomatids. In: Santos da Silva, M. and Cano, M. I. N. (eds.) Molecular and Cellular Biology of Pathogenic Trypanosomatids. Series: Frontiers in parasitology, 1. Bentham Science, pp. 195-260. ISBN 9781681084060 (doi:10.2174/9781681084053117010008)

This list was generated on Sat May 25 13:25:50 2019 BST.