Equipment

JEOL CRYO ARM 300

JEOL CRYO ARM 300 cryo-electron microscope in a lab at the SCMIOur JEOL CRYO ARM 300 is the SCMI's flagship, fully automated cryo-electron microscope. The microscope is used for collecting data for high-resolution single-particle experiments or high-throughput tomography. Users set up their session together with an SCMI staff member to ensure they get the most out of their microscope time. Once set-up, the microscope runs for 48 or 72 hours to automatically collect the data.

  • TEM operating at 300kV
  • 12 sample CRYOSPECPORTER autoloader
  • cold FEG electron source
  • hole-free phase plates
  • in-column omega energy filter
  • Direct Electron DE-64 8k x 8k direct detector
  • SerialEM software for automated data collection

JEOL JEM F200

JEOL F2 cryo electron microscope in a labOur JEOL JEM F200, better known as the 'F2-Cryo' is mostly used to screen samples prior to data collection, although it is a very capable cryo-microscope in its own right.

  • TEM operating at 200kV
  • Gatan 626 side-entry cryo-holder
  • Simple Origin Model 215 dual grid side-entry cryo-holder
  • cold FEG electron source
  • hole-free phase plates
  • Direct Electron DE-20 5120 x 3840 pixel direct detector
  • SerialEM software for automated data collection

JEOL 1200 EX II

JEOL 1200 electron microscope in a labOur JEOL 1200 EX II is used mostly for preliminary negative stain work, or for looking at resin sections. It's easy to learn, with most users able to operate it autonomously after a few sessions.

  • TEM operating at 80kV
  • room temperature side-entry holder for negative stain or resin sections
  • equipped with Gatan Orius CCD camera for collecting images

Sample Preparation Lab

  • SCMI sample preparation equipment on benches in labThermo Fisher Vitrobot Mark IV (2-sided blotting) for cryo-grid preparation of samples in vitrified ice.
  • Leica EM GP2 for single-sided blotting of cryo-grids, under Safety Level 2 conditions.
  • Emitech K100X Glow discharger for optimal preparation of cryo-grids
  • Quorum Q150TE carbon coater