CRYO ARM plus Apollo detector yields 1.85Å reconstruction of tobacco mosaic virus
Published: 13 February 2023
A recent trial of the new Apollo detector on our CRYO ARM 300 microscope yielded a 1.85Å reconstruction of tobacco mosaic virus.
We recently had a demonstration of the new Direct Electron Apollo camera and installed it on our JEOL CRYO ARM 300 microscope. As part of the testing, we collected multiple datasets of common cryo-EM test specimens such as betagalactosidase, apoferritin and tobacco mosaic virus (TMV). These test specimens are commonly used to benchmark microscopes as they are known to lead to high resolution maps and are easy to store and prepare.
While collecting and processing TMV datasets at different magnifcations, it became clear that we'd collected a very high quality dataset that would process to a high resolution reconstruction. Prof David Bhella spent time fully processing the dataset and eventually calculated a 1.85Å map.
Given the interest in seeing results from both the JEOL CRYO ARM 300 and the Direct Electron Apollo, and ensuring there are datasets from different cameras and microscopes available for methods development, the map and underlying micrographs have been deposited in the Electron Microscope Data Bank (EMDB) and Electron Microscopy Public Image Archive (EMPIAR). These are linked to in the links section on the right.
David also shared a video on Twitter, showing a snippet of the map together with an atomic model from Carsten Sachse's lab.
A snippet of density - with coordinates previously determined by @SachseGroup (PDB 6SAE) docked. pic.twitter.com/xnJzeHDnx5
— David Bhella (@Davidbhella) February 8, 2023
First published: 13 February 2023
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