Genome Editing BIOL5376
- Academic Session: 2022-23
- School: School of Molecular Biosciences
- Credits: 10
- Level: Level 5 (SCQF level 11)
- Typically Offered: Semester 2
- Available to Visiting Students: No
- Available to Erasmus Students: No
- Taught Wholly by Distance Learning: Yes
This course aims to introduce students to the range of techniques available for genome editing, including CRISPR, TALENs and ZFNs.
The course will include online practical activities, in which students will follow an experiment to transfect cells with a CRISPR system and select and characterise edited cells. The students will learn about mammalian tissue culture, transfection, genomic DNA purification, PCR and fluorescence microscopy.
The course will also include lectures and journal clubs, focusing on recent developments in research, therapeutics and diagnostics using genome editing and related technologies.
Online only, 3 hours per week lectures/tutorials, plus a series of online practical classes.
70%: short answer questions relating to a genome editing experiment. 1500 words
30%: a graphical abstract showing a plan for a genome editing experiment, informed by the literature.
This course aims to introduce students to a range of different genome editing technologies. It will develop their knowledge of practical techniques so they can plan experiments, interpret data and troubleshoot problems. It will also develop their ability to understand and critically evaluate the details of different technologies and put them into the context of the scientific literature.
Intended Learning Outcomes of Course
By the end of this course students will be able to:
■ Apply their knowledge of relevant molecular and cellular biology techniques to plan a genome editing experiment
■ Critically evaluate different genome editing strategies
■ Critically discuss the literature relating to genome editing
Minimum Requirement for Award of Credits
Students must submit at least 75% by weight of the components (including examinations) of the course's summative assessment.