Recombinant Protein Expression BIOL5305
- Academic Session: 2022-23
- School: School of Molecular Biosciences
- Credits: 10
- Level: Level 5 (SCQF level 11)
- Typically Offered: Semester 1
- Available to Visiting Students: No
- Available to Erasmus Students: No
The course aims to provide students with a critical understanding of the various ways in which large scale protein expression can be carried out in recombinant systems for Biotechnological applications.
Lectures, tutorials and a lab class.
5,000 word lab report
The course aims to provide students with a critical understanding of the various ways in which large scale protein expression can be carried out for Biotechnological applications. The course will explore various recombinant systems, the use of coupled proteins and tags, expression of difficult proteins and different methods of affinity purification.
Intended Learning Outcomes of Course
By the end of this course students will be able to:
■ Critically analyse the current methodologies used to express and purify proteins of biotechnological significance in recombinant systems.
■ Critically evaluate the use of different recombinant systems and purification mechanisms.
■ Describe, critique and perform techniques that are commonly used to express proteins in E.coli.
■ Critically analyse results from experimental data and write an accurate laboratory report using these results.
Minimum Requirement for Award of Credits
Students must submit at least 75% by weight of the components (including examinations) of the course's summative assessment.