School of Infection & Immunity

If you wish to carry out protein expression in E. coli, the facility provides a screening service to help you identify successful expression conditions. A series of plasmids and bacterial strains are available for this purpose.

The plasmid of choice will be used to carry out a preliminary test in a small culture volume (10 ml) using standard conditions (Rosetta 2 pLysS/BL21 pLysS at 18°C, in autoinduction medium).

If soluble protein is detected, further optimization will be carried out. In case of a negative outcome alternative strategies can be explored (e.g. use of a different tag, refolding, co-expression with a protein partner, etc.). Results of expression tests will be further confirmed by western blot upon request.

The screening service is currently available only for proteins fused to the His tag (alone, or in combination with another tag). Cloning is not included in the service.

For further details, please contact Susan Kitson.

Charges will be discussed for individual projects.

A P.O. number (or budget code) will be required upon agreement of protocol before work begins.