Dr Rucha Karnik

  • Royal Society University Research Fellow (Institute of Molecular Cell and Systems Biology)

telephone: 01413301945
email: Rucha.Karnik@glasgow.ac.uk

Biography

I obtained my Bachelor’s degree in Biochemistry (St Xavier’s College, Ahmedabad) and Masters in Biotechnology from the Gujarat University in India, earned with a 1st rank in the Masters programme. I then spent over six years in the pharma-biotech industry where I worked on research, marketing and management aspects of various projects including development of recombinant protein-based diagnostic kits, human therapeutics, biosimilars and the agro-biotech business. Drawn to fundamental research in human disease, I left the industry and moved to the UK to pursue my PhD at the University of Leeds, in the Institute of Membrane and Systems Biology. I was awarded fellowships from the prestigious Overseas Research Students Awards Scheme (ORSAS) sponsored by the British government, the Tetley & Lupton Scholarship and the International Research Scholarship from the University of Leeds for my PhD. At Leeds, I investigated membrane traffic of human K+ channels and its implications for health and disease. For my postdoc, I joined Mike Blatt’s group at the University of Glasgow (2011) where I started working with plants. I have since pursued closely related themes of membrane transport, regulation of membrane traffic and cellular homeostasis, developing a fundamental interest in molecular mechanisms of vesicle traffic and its coordination with transport, which are critical for plant physiology.  As a Royal Society University Research Fellow, the University of Glasgow continues to be my home. To achieve food security in the face of global environment change, it is essential that we improve agricultural crops for efficient growth, nutrient uptake, CO2 and water utilisation and responses to biotic stresses. Therefore, research directed to understanding, predicting, and manipulating the molecular mechanisms of plant growth and responses to the environment is at the forefront of scientific interest. My research focus centres around understainding the role of hormone regulated membrane traffic in plant growth and homeostasis.

Research interests

• Hormone modulate membrane traffic
• Functional regulation of Ion transporters

Technical staff
Mr Craig Bruce

Royal Society Funded PhD Studentship available in the Karnik Lab

Application Deadline: 24th March 2017 or until filled
Start Date: 1st October 2017.

Proton Transport Modulators - Spatial Regulation and Effects on Plant Physiology
Outline and Aim:
One of the biggest challenges faced by the world is sustaining water and food availability to meet the demands of the rapidly increasing human population. Research to understand how plants grow and develop is therefore vital. The ‘acid growth’ hypothesis has long promoted the idea that plasma membrane H+ pumps (H+-ATPases) are regulated to promote acid (H+) transport and generate turgor pressure to drive cell expansion. Mechanisms underlying hormonal control of proton pump traffic and links to functional regulation of the proton pump are unknown. This studentship is funded by a Royal Society grant to identify mechanisms of hormone regulated membrane traffic of effectors that affect functional regulation of the proton transporters at a molecular, cellular and whole organism level.
Expertise:
The student will employ a multidisciplinary approach and gain expertise in cross-platform techniques and concepts in cell biology, molecular cloning, membrane transport and genetics as well as specialised methodologies adapted to study protein-protein interactions using yeast and proteomics. Skills training will include protein biochemistry, in vivo and in vitro protein-protein interaction assays, fluorescence microscopy and optobiology. Electrophysiology studies and training will be carried out in collaboration with Professor Mike Blatt, Regius Professor of Botany, Institute of Molecular, Cell and Systems Biology, at the University of Glasgow. Opportunities to train and learn from the diverse and vibrant research community at the IMCSB in the University of Glasgow will be available as relevant to the project.
Funding: This studentship is funded by a grant from the Royal Society for 4 years. Support includes an annual stipend at RCUK rates for 4 years (£14,296 per year), PhD registration fees at UK/EU student rate (£4121 per year).
Requirements: Applicants must have at least an upper second class BSc or the equivalent degree. Proficiency in basic molecular biology skills is required and knowledge of membrane biology, biochemistry and cell biology is preferred.
Interested candidates should contact Dr Rucha Karnik directly by email at rucha.karnik@glasgow.ac.uk with a CV giving and a brief statement of what they could bring to this project.
References:
1. Hager, A. et al., Planta (1991)185, 527
2. Hager, A. et al., J. of plant res. (2003) 116, 483
3. Spartz, A. et al., Plant Cell (2014) 26, 2129
4. R. Karnik et al., Trends Plant Sci (2017) 22, 81

For formal applications, please visit: http://www.gla.ac.uk/research/opportunities/howtoapplyforaresearchdegree/

 

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Proton Transport Modulators - Spatial Regulation and Effects on Plant Physiology
    Royal Society
    2017 - 2021
     
  • Hormone-Regulated Membrane Traffic and Plant Morphogenesis
    Royal Society
    2016 - 2021
     

Publications

List by: Type | Date

Jump to: 2017 | 2015 | 2014 | 2013 | 2012
Number of items: 9.

2017

Karnik, R., Waghmare, S., Zhang, B. , Larson, E., Lefoulon, C., Gonzalez, W. and Blatt, M. R. (2017) Commandeering channel voltage sensors for secretion, cell turgor, and volume control. Trends in Plant Science, 22(1), pp. 81-95. (doi:10.1016/j.tplants.2016.10.006) (PMID:27818003) (PMCID:PMC5224186)

Zhang, B. , Karnik, R., Waghmare, S., Donald, N. A. and Blatt, M. R. (2017) VAMP721 conformations unmask an extended motif for K+ channel binding and gating control. Plant Physiology, 173(1), pp. 536-551. (doi:10.1104/pp.16.01549) (PMID:27821719)

2015

Grefen, C., Karnik, R., Larson, E., Lefoulon, C., Wang, Y., Waghmare, S., Zhang, B. , Hills, A. and Blatt, M. R. (2015) A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion. Nature Plants, 1, 15108. (doi:10.1038/nplants.2015.108)

Zhang, B. , Karnik, R., Wang, Y., Wallmeroth, N., Blatt, M. R. and Grefen, C. (2015) The arabidopsis R-SNARE VAMP721 interacts with KAT1 and KC1 K+ channels to moderate K+ current at the plasma membrane. Plant Cell, 27(6), pp. 1697-1717. (doi:10.1105/tpc.15.00305) (PMID:26002867) (PMCID:PMC4498211)

Karnik, R., Zhang, B. , Waghmare, S., Aderhold, C., Grefen, C. and Blatt, M. R. (2015) Binding of SEC11 indicates its role in SNARE recycling after vesicle fusion and identifies two pathways for vesicular traffic to the plasma membrane. Plant Cell, 27(3), pp. 675-694. (doi:10.1105/tpc.114.134429) (PMID:25747882)

2014

Lefoulon, C., Karnik, R., Honsbein, A., Gutla, P. V., Grefen, C., Riedelsberger, J., Poblete, T., Dreyer, I., Gonzalez, W. and Blatt, M. R. (2014) Voltage-sensor transitions of the inward-rectifying K+ channel KAT1 indicate a latching mechanism biased by hydration within the voltage sensor. Plant Physiology, 166(2), pp. 960-975. (doi:10.1104/pp.114.244319) (PMID:25185120)

2013

Karnik, R., Grefen, C., Bayne, R., Honsbein, A., Kohler, T., Kioumourtzoglou, D., Williams, M., Bryant, N.J. and Blatt, M.R. (2013) Arabidopsis Sec1/Munc18 protein SEC11 is a competitive and dynamic modulator of SNARE binding and SYP121-dependent vesicle traffic. Plant Cell, 25(4), pp. 1368-1382. (doi:10.1105/tpc.112.108506)

Karnik, A., Karnik, R. and Grefen, C. (2013) SDM-Assist software to design site-directed mutagenesis primers introducing “silent” restriction sites. BMC Bioinformatics, 14, 105. (doi:10.1186/1471-2105-14-105) (PMID:23522286) (PMCID:PMC3644487)

2012

Wang, Y., Papanatsiou, M., Eisenach, C., Karnik, R., Williams, M., Hills, A., Lew, V.L. and Blatt, M.R. (2012) Systems dynamic modeling of a guard cell Cl- channel mutant uncovers an emergent homeostatic network regulating stomatal transpiration. Plant Physiology, 160(4), pp. 1956-1967. (doi:10.1104/pp.112.207704)

This list was generated on Thu Aug 17 19:56:41 2017 BST.
Jump to: Articles
Number of items: 9.

Articles

Karnik, R., Waghmare, S., Zhang, B. , Larson, E., Lefoulon, C., Gonzalez, W. and Blatt, M. R. (2017) Commandeering channel voltage sensors for secretion, cell turgor, and volume control. Trends in Plant Science, 22(1), pp. 81-95. (doi:10.1016/j.tplants.2016.10.006) (PMID:27818003) (PMCID:PMC5224186)

Zhang, B. , Karnik, R., Waghmare, S., Donald, N. A. and Blatt, M. R. (2017) VAMP721 conformations unmask an extended motif for K+ channel binding and gating control. Plant Physiology, 173(1), pp. 536-551. (doi:10.1104/pp.16.01549) (PMID:27821719)

Grefen, C., Karnik, R., Larson, E., Lefoulon, C., Wang, Y., Waghmare, S., Zhang, B. , Hills, A. and Blatt, M. R. (2015) A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion. Nature Plants, 1, 15108. (doi:10.1038/nplants.2015.108)

Zhang, B. , Karnik, R., Wang, Y., Wallmeroth, N., Blatt, M. R. and Grefen, C. (2015) The arabidopsis R-SNARE VAMP721 interacts with KAT1 and KC1 K+ channels to moderate K+ current at the plasma membrane. Plant Cell, 27(6), pp. 1697-1717. (doi:10.1105/tpc.15.00305) (PMID:26002867) (PMCID:PMC4498211)

Karnik, R., Zhang, B. , Waghmare, S., Aderhold, C., Grefen, C. and Blatt, M. R. (2015) Binding of SEC11 indicates its role in SNARE recycling after vesicle fusion and identifies two pathways for vesicular traffic to the plasma membrane. Plant Cell, 27(3), pp. 675-694. (doi:10.1105/tpc.114.134429) (PMID:25747882)

Lefoulon, C., Karnik, R., Honsbein, A., Gutla, P. V., Grefen, C., Riedelsberger, J., Poblete, T., Dreyer, I., Gonzalez, W. and Blatt, M. R. (2014) Voltage-sensor transitions of the inward-rectifying K+ channel KAT1 indicate a latching mechanism biased by hydration within the voltage sensor. Plant Physiology, 166(2), pp. 960-975. (doi:10.1104/pp.114.244319) (PMID:25185120)

Karnik, R., Grefen, C., Bayne, R., Honsbein, A., Kohler, T., Kioumourtzoglou, D., Williams, M., Bryant, N.J. and Blatt, M.R. (2013) Arabidopsis Sec1/Munc18 protein SEC11 is a competitive and dynamic modulator of SNARE binding and SYP121-dependent vesicle traffic. Plant Cell, 25(4), pp. 1368-1382. (doi:10.1105/tpc.112.108506)

Karnik, A., Karnik, R. and Grefen, C. (2013) SDM-Assist software to design site-directed mutagenesis primers introducing “silent” restriction sites. BMC Bioinformatics, 14, 105. (doi:10.1186/1471-2105-14-105) (PMID:23522286) (PMCID:PMC3644487)

Wang, Y., Papanatsiou, M., Eisenach, C., Karnik, R., Williams, M., Hills, A., Lew, V.L. and Blatt, M.R. (2012) Systems dynamic modeling of a guard cell Cl- channel mutant uncovers an emergent homeostatic network regulating stomatal transpiration. Plant Physiology, 160(4), pp. 1956-1967. (doi:10.1104/pp.112.207704)

This list was generated on Thu Aug 17 19:56:41 2017 BST.