Professor Harry De Koning

  • Professor of Parasite Biochemistry and Pharmacology (Parasitology)
  • Associate - Life Sciences (School of Life Sciences)

telephone: 01413303753
email: Harry.De-Koning@glasgow.ac.uk

Research interests

The two main aspects of my research are the investigation of the mechanisms of drug resistance in protozoan parasites, mainly African trypanosomes, and the identification of new lead compounds and therapeutic strategies against various protozoan pathogens including Trypanosoma brucei, Trypanosoma congolense, Leishmania species and Trichomonas vaginalis.

The two most important classes of drugs against African trypanosomiasis in humans (also called sleeping sickness) and animals are commonly known as aromatic diamidines and melaminophenyl arsenicals. Both classes of drugs are old, dating from the 1930s and their long usage has inevitably led to highly significant levels of resistance in the parasites. We have investigated the mechanisms by which the trypanosomes become resistant to the drugs and found this is mostly linked to the loss of specific transport proteins; in their absence the drugs do not enter the parasite and are therefore ineffective. We have identified and characterised these transporters and ongoing work is identifying the genes encoding them so that genetic research can be performed on parasite populations. We further work on the identification of the Trypanosoma brucei resistance mechanism for isometamidium, an important phenanthridine drug against animal trypanosomiasis in sub-Saharan Africa.

We have developed much needed screening tools for drug efficacy to some parasites, including Leishmania species, Trypanosoma species and Trichomonas vaginalis. These enable the screening of compound collections for activity against these pathogens. We are working together with academic collaborators and pharmaceutical companies to discover new lead compounds for pre-clinical drug development. Several series of compounds have been tested and promising leads have been identified, especially against Trypanosoma species.

Finally, our emphasis on transporters of protozoan parasites is not limited to drug transporters per se. I have worked on nutrient transporters, particularly purine and pyrimidine transporters since 1994 and contributed much to our knowledge how protozoa take up these vital nutrients from their hosts. Having recently characterised pyrimidine transport activities in several protozoa, current projects include the identification of pyrimidine transporter genes in Trypanosoma and Leishmania species, and the characterisation of nucleoside and nucleobase transporters in Trichomonas vaginalis.

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • A new drug discovery pipeline for animal African trypanosomiasis
    Biotechnology and Biological Sciences Research Council
    2016 - 2019
     
  • A new drug discovery pipeline for animal African trypanosomiasis
    GALVmed
    2016 - 2019
     
  • Drug resistance and cross-resistance in livestock trypanosomes.
    Bill and Melinda Gates Foundation
    2016 - 2016
     
  • Fellowship programme in Infection and Immunity
    Royal Embassy of Saudi Arabia
    2015 - 2016
     
  • Drug resistance and cross-resistance in livestock trypanosomes.
    GALVmed
    2014 - 2016
     
  • PDE4NTD: Phosphodieasease inhibitors for the treatment of Neglected Parasitic Diseases.
    European Commission
    2014 - 2018
     
  • Diamidine and oxaborole transporters and resistance in livestock tryponosomes (Gap Period)
    Gates Foundation
    2014 - 2014
     
  • Modulation of trypanosomal cAMP signaling for sleeping sickness therapeutic therapy
    Tres Cantos Open Lab Foundation
    2014 - 2015
     
  • Modulation of trypanosomal cAMP signaling for sleeping sickness therapeutic therapy
    Tres Cantos Open Lab Foundation
    2014 - 2015
     
  • Diamidine transporters and resistance in livestock trypanosomes.
    GALVmed
    2013 - 2014
     
  • Diamidine transporters and resistance in livestock trypanosomes
    Department for International Development
    2012 - 2013
     
  • Uptake of novel diamidine drugs by Trypanosoma brucei
    Royal Society
    2009 - 2011
     
  • Drug resistance in African trypanosomes
    MRC
    2008 - 2011
     
  • Fellowship programme in Infection and Immunity
    Royal Embassy of Saudi Arabia
    2008 - 2014
     
  • Assessment of the probability of developing resistance to novel trypanocides under consideration for late stage HAT
    The University of North Carolina at Chapel Hill
    2006 - 2008
     
  • Combatting drug resistance in human African trypanosomiasis
    UBS-Optimus
    2004 - 2007
     

Additional information

Grant Advisory Board

  • 2009 - 2012: NANOTRYP - Scientific Advisory Board
  • 2008 - 2009: Cyprus Research Promotion Foundation - Panel member for 'Strategic Infrastructure Projects'
  • 2008 - ongoing: European Science Foundation - Member, International Evaluation Committee of the Cyprus Research Promotion Foundation (Grant Review panel)
  • 2007 - ongoing: World Organisation for Animal Health - Member, OiE ad hoc group on Non-Tsetse Transmitted Animal Trypanosomoses (NTTAT)
  • 2005 - 2008: Pharmalinks - Member of Management Committee
  • 2004 - ongoing: CURE IT - Co-chair of the CURE IT collaborative consortium on anti-trypanosomal chemotherapy with the University of Strathclyde as part of the Synergy initiative
  • 2003 - ongoing: Medecins-sans-Frontier - Member of Neglected Diseases Group Advisory Board to the Drugs for Neglected Diseases Initiative

Invited International Presentations

  • 2014: Umea, Sweden - University of Umea, Sweden. Drug resistance mechanisms in a protozoan parasite, Trypanosoma brucei. 28 January 2014
  • 2014: Malaga, Spain - International Conference on Global Challenges for New Drug Discovery Against Tropical Parasitic Diseases. February 17-20, 2014. Aquaglyceroporins determine sensitivity to pentamidine and melarsoprol in Trypanosoma brucei.
  • 2014: Al-Baha, Saudi Arabia - 1st Scientific Research Conference, Al-Baha University March 18th, 2014. Scientific research - From idea to publication.
  • 2014: Al-Baha, Saudi Arabia - 1st Scientific Research Conference, Al-Baha University March 17th, 2014. Ethics and considerations for a successful research programme.
  • 2014: Cambridge, England, UK - Annual British Society for Parasitology Spring Conference. April 6th-9th, 2014. Aquaporin 2 is the main determinant for pentamidine and melaminophenyl arsenical resistance in Trypanosoma brucei spp
  • 2014: 7th Saudi Students Scientific Conference. Feb 1st - 2nd, 2014. Invited speaker and session Chair. Research into parasitic diseases of Saudi Arabia and the Middle East by Saudi students at the University of Glasgow.
  • 2014: Madrid, Spain - Towards New Therapeutics for Diseases of the Developing World”. May 11th-13th 2014, Tres Cantos. The importance of transporters in drug action and resistance: examples form African trypanosomes.
  • 2013: Madrid, Spain - 15th International Symposium on Purine and Pyrimidine Metabolism in Man. June 9-13, 2013. Evaluation of pyrimidine metabolism as a drug target in kinetoplastid parasites. Invited lecture and session Chair
  • 2013: Stockholm, Sweden - Karolinska Institute. Pyrimidine transporters and metabolism in Trypanosoma brucei - opportunities and bottlenecks. 20 Feb 2012.
  • 2013: Glasgow, Scotland, UK - University of Glasgow Parasitology Networking Day. Aquaporins are the principal determinant for pentamidine and melarsoprol sensitivity in Trypanosoma brucei. 20 August 2013
  • 2013: St Andrews, Scotland - St Andrews University. Pyrimidine transporters and metabolism in Trypanosoma brucei - where are the drug targets? 3 April, 2013.
  • 2013: Amsterdam, The Netherlands - 45th Microsymposium on Glycosomes and Parasite/Trypanosomatid Metabolism. October 10th-11th, 2013. The role of T. brucei Aquaporins in drug sensitivity and resistance.
  • 2012: Antwerp, Belgium - Institute for Tropical Medicine. Mechanisms of diamidine and phenanthridine resistance in Trypanosoma brucei. January 26th, 2012.
  • 2012: Munich, Germany - Ludwig-Maximilians-Universitat Munchen, Department of Genetics. Markers and determinants of drug resistance in a model protozoan pathogen, Trypanosoma brucei. January 24th, 2012.
  • 2012: Ceske Budejovice, Czech Republic - Czech Institute of Parasitology. Evaluation of pyrimidine biosynthesis and salvage as drug targets in African trypanosomes. 18 October 2012
  • 2012: Barcelona, Spain - University of Barcelona. Pyrimidine transporters and metabolism in Trypanosoma brucei - where are the drug targets? Dec 21st 2012
  • 2012: Antwerp, Belgium - International Symposium ‘African trypanosomes’, September 14, 2012. Institute Tropical Medicine. Drug design: the past, the present and future. Invited lecture
  • 2012: Edinburgh, Scotland - ‘Diamidines drug resistance in Animal Trypanosomosis’ meeting by GALVmed.24 October 2012. The role of TcoAT1 in diminazene resistance of Trypanosoma congolense. Invited oral presentation
  • 2012: Cambridge, England, UK - Sanger Institute. Pyrimidine metabolism in kinetoplastids: a validated drug target. January 23rd, 2012.
  • 2012: London, England, UK - London School of Hygiene and Tropical Medicine. Assessment of pyrimidine biosynthesis and salvage pathways as drug targets in Trypanosoma brucei: pipeline or pipe dream? 1 November 2012.
  • 2011: Riviera Mayo, Mexico - Global Challenges for New Drug Discovery Against Tropical Parasitic Disease, December 11-15, 2011
  • 2011: Tbilisi, Georgia - National Centers for Disease Control. Drug resistance mechanisms in African trypanosomes. November 28th, 2011
  • 2011: Hull, England - Northern UK Kinetoplastid Forum (NUKK5) May 6th, 2011. cAMP phosphodiesterases of Trypanosoma brucei: validation as targets for chemotherapy. Invited keynote lecture
  • 2011: Amsterdam, The Netherlands - Netherlands Cancer Institute. The role of transporters in chemotherapy. October 26th, 2011
  • 2010: Bournemouth, UK - British Blood Transfusion Society 28th Annual Conference, Red Cell Special Interest Group. Invited oral presentation: 'Purine and pyrimidine transport in healthy and Plasmodium falciparum-infected human erythrocytes'
  • 2010: Madrid, Spain - Invited Seminar at Institute for Medicinal Chemistry, 'Choline-derived analogues as new antiprotozoal lead compounds'
  • 2010: Hull, UK - Northern UK Kinetoplastid Forum (NUKK5). Keynote lecture: 'cAMP phosphodiesterases of Trypanosoma brucei: validation as targets for chemotherapy'
  • 2010: Dundee, Scotland - Epithelial Biology 2010. Keynote address: 'Nutrient transport in protozoan parasites'
  • 2010: Waterville Valley, NH, USA - Gordon Research Conference on Cyclic Nucleotide Phosphodiesterases. Invited oral presentation: 'Phosphodiesterase inhibitors reveal a critical role for cAMP in the cell division cycle of Trypanosoma brucei'
  • 2010: Tarragona, Spain - Purines 2010: Adenine nucleosides and nucleotides in Biomedicine. Invited oral presentation: 'Molecular modelling of substrate recognition at an unusual aminopurine transporter'
  • 2010: Barcelona, Spain - International Workshop on Nucleoside and nucleobase transporter proteins in biomedicine. Invited Lecture: 'Transport in Plasmodium-infected erythrocytes
  • 2010: Granada, Spain - Invited Seminar at Instituto de Parasitologia y Biomedicina "Lopez-Neyra". Drug targeting and transport mechanisms in pathogenic protozoa'
  • 2009: Madrid, Spain - Institute for Medicinal Chemistry - Invited seminar: Modelling of substrate interactions with membrane proteins of unknown structure
  • 2009: Cambridge, UK - University of Cambridge, Department of Pathology. cAMP signalling in Trypanosoma brucei: a target for novel chemotherapy
  • 2009: Brussels, Belgium - Free University - Trypanosoma brucei: Transporters and chemotherapy.
  • 2009: Basel, Switzerland - Swiss tropical Institute, Basel - Drug resistance mechanisms and new therapeutic strategies in Human African Trypanosomiasis
  • 2009: St Andrews, Scotland - Annual Conference of the Consortium for Parasitic Drug Design. Invited oral presentation 'Transporters in drug uptake and resistance'
  • 2009: Amsterdam, The Netherlands - 'Neglected Diseases Seminar' for Top Institute Pharma. 'Drug resistance and drug development in parasitic protozoa: the role of transporters'
  • 2009: Bonn, Germany - University of Bonn - Strategies for the study of nucleobase and nucleoside transporters in protozoa
  • 2008: Bagamoyo, Tanzania - Annual meeting for the Consortium for Parasitic Drug Development. - "Diminazene uptake by the High Affinity Pentamidine Transporter"
  • 2008: Bagamoyo, Tanzania - Annual meeting for the Consortium for Parasitic Drug Development. - "New lead compounds against African trypanosomes."
  • 2008: Lucca, Italy - Gordon Research Conference on Cyclic Nucleotide Phosphodiesterases - "The mechanism of action of PDE inhibitors in Trypanosoma brucei: rapid elevation of cAMP levels leads to cell death through highly specific actions on cell "
  • 2007: Atlanta, USA - Diamidine transporters in Trypanosoma brucei. Annual meeting for the Consortium for Parasitic Drug Development [Gates Foundation / WHO].
  • 2007: Modena, Italy - Curcumin analogues as a new class of antiprotozoals; activities and investigation of mechanism of action. COST B22 Working group1/3 Expert Group Meeting: Medicinal Chemistry in Parasitology: new avenues in drug discovery
  • 2007: Ceske Budejovice, Czech Republic - The role of transporters in antiparasitic drug design. Research Seminar at the Parasitology Institute.
  • 2007: University of Glasgow, Glasgow, UK - Co-organiser and chairman/moderator at PharmaLinks 'Chemistry and Biology into Medicine'
  • 2007: Dundee, Scotland - SAR of determinants of diamidine transport and resistance in African trypanosomes. COST B22 Annual Congress: Drug development for parasitic diseases.
  • 2006: University of Montpellier, France - Presentation: Strategies for chemotherapy of parasitic diseases.
  • 2006: Glasgow, Scotland - Member of organising committee of ICOPA IX (11th International Conference of Parasitology)
  • 2006: Dakar, Senegal - Symposium Organiser - The Incidence, Causes and Mechanisms of Drug Resistance in African Trypanosomiasis. Kinetoplastid Diseases
  • 2006: Glasgow, Scotland - Co-organiser Pharmalinks Chemistry-Biology Day, Strathclyde University and Glasgow University
  • 2006: Basel, Switzerland - The use of transporter mutants in antiprotozoal drug screening. COST B22 Working group 3/4 Expert Group Meeting: Drug development for parasitic diseases.
  • 2005: Switzerland - Invited plenary lecture: The ENT family of transporters in Trypanosoma brucei - its role in drug action and resistance'. 21st Swiss trypanosomatid meeting.
  • 2005: Boxmeer, The Netherlands - Intervet International, 'Trypanocides: Transporters, resistance mechanisms and some new strategies.'
  • 2004: Glasgow, Scotland - Co-organiser of the combined CURE-IT and DNDi [Drugs-for-Neglected-Diseases-initiative] meeting 'Sleeping sickness and the brain', University of Glasgow Veterinary School
  • 2004: Antwerp, Belgium - COST B22 Meeting on drug development for parasitic diseases.
  • 2004: Invited oral presentation: 'Delivering the magic bullet: the role of transporters in rational drug design.' World Conference on Dosing of Antiinfectives - Dosing the Magic Bullets
  • 2004: Academy of Sciences of the Czech Republic, Prague - 'The ENT family of purine transporters in protozoa: Cloning, characterisation and identification of substrate-transporter interactions responsible for very high activity.'
  • 2004: Athens, Greece - Series of lectures on Biochemistry, molecular biology and genomics of pathogenic protozoa for postgraduate students at the National University of Athens
  • 2004: Ceske Budejovice, Czech Republic - Transport and resistance of arsenicals and diamidines in Trypanosoma brucei. British Society for Parasitology Trypanosomiasis and Leishmaniasis Seminar
  • 2004: Antwerp, Belgium - Invited plenary lecture: 'Diamidine transporters and resistance in protozoa.' COST B22 meeting on "Drug development for Parasitic Diseases"
  • 2002: University of Athens, Greece - The significance of transporters in chemotherapy
  • 2001: Chania, Greece - Protozoan nucleobase transporters: substrate recognition motifs reveal selective uptake of purine antimetabolites, invited plenary lecture at the 19th Small meeting on Yeast transporters and energetics
  • 2001: Finlay Institute, Havana, Cuba - Protozoan nucleobase transporters: substrate recognition motifs reveal selective uptake of purine antimetabolites

Professional Learned Society

  • 2005 - ongoing: Netherlands Society for Parasitology - Member
  • 1997 - ongoing: British Society for Parasitology - Member
  • 1995 - ongoing: Biochemical Society - Member

Research Fellowship

  • 1999 - 2003: Wellcome Trust Research Fellowship

Publications

List by: Type | Date

Jump to: 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001 | 2000 | 1999 | 1998 | 1997 | 1994 | 1993 | 1992 | 1991 | 1988
Number of items: 108.

2016

Vodnala, M., Ranjbarian, F., Pavlova, A., de Koning, H. P., and Hofer, A. (2016) Trypanosoma brucei methylthioadenosine phosphorylase protects the parasite from the antitrypanosomal effect of deoxyadenosine. Journal of Biological Chemistry, 291(22), pp. 11717-11726. (doi:10.1074/jbc.M116.715615) (PMID:27036940)

Dike, V. T., Vihiior, B., Bosha, J. A., Yin, T. M., Ebiloma, G. U., de Koning, H. P., Igoli, J. O., and Gray, A. I. (2016) Antitrypanosomal activity of a novel taccalonolide from the tubers of Tacca leontopetaloides. Phytochemical Analysis, 27(3-4), pp. 217-221. (doi:10.1002/pca.2619) (PMID:27313159)

Imamura, H. et al. (2016) Evolutionary genomics of epidemic visceral leishmaniasis in the Indian subcontinent. eLife, 5, e12613. (doi:10.7554/eLife.12613) (PMID:27003289) (PMCID:PMC4811772)

Graf, F. E. et al. (2016) Comparative genomics of drug resistance in Trypanosoma brucei rhodesiense. Cellular and Molecular Life Sciences, (doi:10.1007/s00018-016-2173-6) (PMID:26973180) (Early Online Publication)

Omar, R. M.K., Igoli, J., Gray, A. I., Ebiloma, G. U., Clements, C., Fearnley, J., Edrada Ebel, R. A., Zhang, T., De Koning, H., and Watson, D. (2016) Chemical characterisation of Nigerian red propolis and its biological activity against Trypanosoma Brucei. Phytochemical Analysis, 27(2), pp. 107-115. (doi:10.1002/pca.2605) (PMID:26662866)

Babuadze, G. et al. (2016) Seroepidemiology and molecular diversity of Leishmania donovani complex in Georgia. Parasites and Vectors, 9, 279. (doi:10.1186/s13071-016-1558-6) (PMID:27177688) (PMCID:PMC4866401)

Siheri, W. et al. (2016) Chemical and antimicrobial profiling of propolis from different regions within Libya. PLoS ONE, 11(5), e0155355. (doi:10.1371/journal.pone.0155355) (PMID:27195790) (PMCID:PMC4873177)

2015

Alkhaldi, A. A.M., Martinek, J., Panicucci, B., Dardonville, C., Zikova, A., and De Koning, H. (2015) Trypanocidal action of bisphosphonium salts through a mitochondrial target in bloodstream form Trypanosoma brucei. International Journal for Parasitology: Drugs and Drug Resistance, 6(1), pp. 23-34. (doi:10.1016/j.ijpddr.2015.12.002)

Rodenko, B., Wanner, M., Alkhaldi, A., Ebiloma, G., Barnes, R., Kaiser, M., Brun, R., McCulloch, R., Koomen, G.-J., and De Koning, H. P. (2015) Targeting the parasite's DNA with methyltriazenyl purine analogs Is a safe, selective, and efficacious antitrypanosomal strategy. Antimicrobial Agents and Chemotherapy, 59(11), pp. 6708-6716. (doi:10.1128/aac.00596-15) (PMID:26282430) (PMCID:PMC4604408)

Ríos Martínez, C. H., Nué Martínez, J. J., Ebiloma, G. U., De Koning, H. P., Alkorta, I., and Dardonville, C. (2015) Lowering the pKa of a bisimidazoline lead with halogen atoms results in improved activity and selectivity against Trypanosoma brucei in vitro. European Journal of Medicinal Chemistry, 101, pp. 806-817. (doi:10.1016/j.ejmech.2015.07.013) (PMID:26231081)

Graf, F. E., Baker, N., Munday, J. C., De Koning, H. P., Horn, D., and Mäser, P. (2015) Chimerization at the AQP2–AQP3 locus is the genetic basis of melarsoprol–pentamidine cross-resistance in clinical Trypanosoma brucei gambiense isolates. International Journal for Parasitology: Drugs and Drug Resistance, 5(2), pp. 65-68. (doi:10.1016/j.ijpddr.2015.04.002) (PMID:26042196) (PMCID:PMC4443405)

Munday, J. C. et al. (2015) Functional analysis of drug resistance-associated mutations in the Trypanosoma brucei Adenosine Transporter 1 (TbAT1) and the proposal of a structural model for the protein. Molecular Microbiology, 96(4), pp. 887-900. (doi:10.1111/mmi.12979) (PMID:25708978)

Alkhaldi, A.A.M., Creek, D.J., Ibrahim, H., Kim, D.-H., Quashie, N.B., Burgess, K.E., Changtam, C., Barrett, M.P., Suksamrarn, A., and De Koning, H.P. (2015) Potent Trypanocidal curcumin analogs bearing a monoenone linker motif act on Trypanosoma brucei by forming an adduct with Trypanothione. Molecular Pharmacology, 87(3), pp. 451-464. (doi:10.1124/mol.114.096016)

Ríos Martínez, C. H., Miller, F., Ganeshamoorthy, K., Glacial, F., Kaiser, M., De Koning, H., Eze, A. A., Lagartera, L., Herraiz, T., and Dardonville, C. (2015) A new nonpolar N-hydroxy imidazoline lead compound with improved activity in a murine model of late-stage Trypanosoma brucei brucei infection is not cross-resistant with diamidines. Antimicrobial Agents and Chemotherapy, 59(2), pp. 890-904. (doi:10.1128/AAC.03958-14)

Shameer, S. et al. (2015) TrypanoCyc: a community-led biochemical pathways database for Trypanosoma brucei. Nucleic Acids Research, 43(D1), D637-D644. (doi:10.1093/nar/gku944)

Dardonville, C., Alkhaldi, A. A.M., and De Koning, H. P. (2015) SAR studies of diphenyl cationic trypanocides: superior activity of phosphonium over ammonium salts. ACS Medicinal Chemistry Letters, 6(2), pp. 151-155. (doi:10.1021/ml500408d)

Munday, J. C., Settimo, L., and De Koning, H. P. (2015) Transport proteins determine drug sensitivity and resistance in a protozoan parasite, Trypanosoma brucei. Frontiers in Pharmacology, 6, 32. (doi:10.3389/fphar.2015.00032) (PMID:24235095) (PMCID:PMC3922157)

Natto, M. J., Eze, A. A., and De Koning, H. P. (2015) Protocols for the routine screening of drug sensitivity in the human parasite Trichomonas vaginalis. Methods in Molecular Biology, 1263, pp. 103-110. (doi:10.1007/978-1-4939-2269-7_8)

Tagoe, D. N. A., Kalejaiye, T. D., and De Koning, H. P. (2015) The ever unfolding story of cAMP signaling in trypanosomatids: vive la difference! Frontiers in Pharmacology, 6, 185. (doi:10.3389/fphar.2015.00185) (PMID:26441645) (PMCID:PMC4561360)

Unciti-Broceta, J. D., Arias, J. L., Maceira, J., Soriano, M., Ortiz-González, M., Hernández-Quero, J., Muñóz-Torres, M., De Koning, H. P., Magez, S., and Garcia-Salcedo, J. A. (2015) Specific cell targeting therapy bypasses drug resistance mechanisms in African trypanosomiasis. PLoS Pathogens, 11(6), e1004942. (doi:10.1371/journal.ppat.1004942) (PMID:26110623) (PMCID:PMC4482409)

2014

Acosta-Reyes, F. J., Dardonville, C., De Koning, H. P., Natto, M., Subirana, J. A., and Campos, J. L. (2014) In and out of the minor groove: interaction of an AT-rich DNA with the drug CD27. Acta Crystallographica. Section D: Biological Crystallography, 70(6), pp. 1614-1621. (doi:10.1107/S139900471400697X)

Lüscher, A., Lamprea-Burgunder, E., Graf, F. E., De Koning, H., and Mäser, P. (2014) Trypanosoma brucei adenine-phosphoribosyltransferases mediate adenine salvage and aminopurinol susceptibility but not adenine toxicity. International Journal for Parasitology: Drugs and Drug Resistance, 4(1), pp. 55-63. (doi:10.1016/j.ijpddr.2013.12.001)

Munday, J. C. et al. (2014) Trypanosoma brucei aquaglyceroporin 2 is a high-affinity transporter for pentamidine and melaminophenyl arsenic drugs and the main genetic determinant of resistance to these drugs. Journal of Antimicrobial Chemotherapy, 69(3), pp. 651-663. (doi:10.1093/jac/dkt442) (PMID:24235095) (PMCID:PMC3922157)

Babuadze, G. et al. (2014) Epidemiology of Visceral Leishmaniasis in Georgia. PLoS Neglected Tropical Diseases, 8(3), e2725. (doi:10.1371/journal.pntd.0002725)

Garcia-Salcedo, J. A., Munday, J. C., Unciti-Broceta, J. D., and De Koning, H. P. (2014) Progress towards new treatments for human African trypanosomiasis. In: Magez, S. and Radwanska, M. (eds.) Trypanosomes and Trypanosomiasis. Springer, pp. 217-238. ISBN 9783709115558 (doi:10.1007/978-3-7091-1556-5_9)

Zimmermann, S. C., O'Neill, E., Ebiloma, G. U., Wallace, L. J.M., De Koning, H. P., and Seley-Radtke, K. L. (2014) Design and synthesis of a series of truncated neplanocin fleximers. Molecules, 19(12), pp. 21200-21214. (doi:10.3390/molecules191221200)

2013

Munday, J.C., Rojas López, K.E., Eze, A.A., Delespaux, V., Van Den Abbeele, J., Rowan, T., Barrett, M.P., Morrison, L.J., and De Koning, H.P. (2013) Functional expression of TcoAT1 reveals it to be a P1-type nucleoside transporter with no capacity for diminazene uptake. International Journal for Parasitology: Drugs and Drug Resistance, 3, pp. 69-76. (doi:10.1016/j.ijpddr.2013.01.004)

Vodnala, S.K. et al. (2013) Structure–activity relationships of synthetic cordycepin analogues as experimental therapeutics for African trypanosomiasis. Journal of Medicinal Chemistry, 56(24), pp. 9861-9873. (doi:10.1021/jm401530a)

Graf, F. E., Wenzler, T., Ludin, P., Kaiser, M., Brun, R., Pyana, P. P., Buscher, P., De Koning, H. P., Horn, D., and Maser, P. (2013) Aquaporin 2 mutations in Trypanosoma brucei gambiense field isolates correlate with decreased susceptibility to pentamidine and melarsoprol. PLoS Neglected Tropical Diseases, 7(10), e2475. (doi:10.1371/journal.pntd.0002475)

Gould, M.K., Bachmaier, S., Ali, J.A.M., Alsford, S., Tagoe, D.N.A., Munday, J.C., Schnaufer, A.C., Horn, D., Boshart, M., and De Koning, H. (2013) Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNA interference library screening for resistance to the phosphodiesterase inhibitor CpdA. Antimicrobial Agents and Chemotherapy, 57(10), pp. 4882-4893. (doi:10.1128/AAC.00508-13)

Baker, N., De Koning, H.P., Mäser, P., and Horn, D. (2013) Drug resistance in African trypanosomiasis: the melarsoprol and pentamidine story. Trends in Parasitology, 29(3), pp. 110-118. (doi:10.1016/j.pt.2012.12.005)

Ali, J.A.M., Creek, D.J., Burgess, K., Allison, H.C., Field, M.C., Maser, P., and De Koning, H.P. (2013) Pyrimidine salvage in trypanosoma brucei bloodstream forms and the trypanocidal action of halogenated pyrimidines. Molecular Pharmacology, 83(2), pp. 439-453. (doi:10.1124/mol.112.082321) (PMID:23188714)

Ali, J. A.., Tagoe, D. N.A., Munday, J. C., Donachie, A., Morrison, L. J., and De Koning, H. P. (2013) Pyrimidine biosynthesis is not an essential function for trypanosoma brucei bloodstream forms. PLoS ONE, 8(3), e58034. (doi:10.1371/journal.pone.0058034) (PMID:23505454) (PMCID:PMC3591441)

Delespaux, V., and De Koning, H. (2013) Transporters in anti-parasitic drug development and resistance. In: Jäger, T., Koch, O. and Flohé, L. (eds.) Trypanosomatid Diseases: Molecular Routes to Drug Discovery. Series: Drug discovery in infectious diseases (4). John Wiley and Sons Ltd.: Weinheim, pp. 335-349. ISBN 9783527332557 (doi:10.1002/9783527670383.ch18)

Rodenko, B., and De Koning, H. (2013) Rational selection of anti-microbial drug targets: unique or conserved? In: Jäger, T., Koch, O. and Flohé, L. (eds.) Trypanosomatid Diseases: Molecular Routes to Drug Discovery. Series: Drug discovery in infectious diseases (4). Wiley-Blackwell: Weinheim, pp. 279-296. ISBN 9783527332557 (doi:10.1002/9783527670383.ch15)

2012

Baker, N., Glover, L., Munday, J. C., Aguinaga Andres, D., Barrett, M. P., De Koning, H. P., and Horn, D. (2012) Aquaglyceroporin 2 controls susceptibility to melarsoprol and pentamidine in African trypanosomes. Proceedings of the National Academy of Sciences of the United States of America, 109(27), pp. 10996-11001. (doi:10.1073/pnas.1202885109)

De Koning, H.P., Gould, M.K., Sterk, G.J., Tenor, H., Kunz, S., Luginbuehl, E., and Seebeck, T. (2012) Pharmacological validation of Trypanosoma brucei phosphodiesterases as novel drug targets. Journal of Infectious Diseases, 206(2), pp. 229-237. (doi:10.1093/infdis/jir857)

Natto, M.J., Savioli, F., Quashie, N.B., Dardonville, C., Rodenko, B., and De Koning, H.P. (2012) Validation of novel fluorescence assays for the routine screening of drug susceptibilities of Trichomonas vaginalis. Journal of Antimicrobial Chemotherapy, 67(4), pp. 933-943. (doi:10.1093/jac/dkr572)

Taladriz, A. et al. (2012) Synthesis and structure–activity analysis of new phosphonium salts with potent activity against African Trypanosomes. Journal of Medicinal Chemistry, 55(6), pp. 2606-2622. (doi:10.1021/jm2014259)

2011

Teka, I. A. et al. (2011) The diamidine diminazene aceturate is a substrate for the high-affinity pentamidine transporter: implications for the development of high resistance levels in trypanosomes. Molecular Pharmacology, 80(1), pp. 110-116. (doi:10.1124/mol.111.071555)

Ibrahim, H.M.S., Al-Salabi, M.I., El Sabbagh, N., Quashie, N.B., Alkhaldi, A.A.M., Escale, R., Smith, T.K., Vial, H.J., and De Koning, H.P. (2011) Symmetrical choline-derived dications display strong anti-kinetoplastid activity. Journal of Antimicrobial Chemotherapy, 66(1), pp. 111-125. (doi:10.1093/jac/dkq401)

Gould, M.K., and De Koning, H.P. (2011) Cyclic-nucleotide signalling in protozoa. FEMS Microbiology Reviews, 31(3), pp. 515-541. (doi:10.1111/j.1574-6976.2010.00262.x)

Rodenko, B., Al-Salabi, M.I., Teka, I.A., Ho, W., El-Sabbagh, N., Ali, J.A.M., Ibrahim, H.M.S., Wanner, M.J., Koomen, G.-J., and De Koning, H.P. (2011) Synthesis of marine-derived 3-alkylpyridinium alkaloids with potent antiprotozoal activity. ACS Medicinal Chemistry Letters, 2(12), pp. 901-906. (doi:10.1021/ml200160k)

Shakur, Y., De Koning, H.P., Ke, H., Kambayashi, J., and Seebeck, T. (2011) Therapeutic potential of phosphodiesterase inhibitors in parasitic diseases. Handbook of Experimental Pharmacology, 204, pp. 487-510. (doi:10.1007/978-3-642-17969-3_20)

Ward, C.P., Wong, P.E., Burchmore, R.J.S., De Koning, H.P., and Barrett, M.P. (2011) Trypanocidal furamidine analogues: influence of pyridine nitrogens on trypanocidal activity, transport kinetics, and resistance patterns. Antimicrobial Agents and Chemotherapy, 55(5), pp. 2352-2361. (doi:10.1128/AAC.01551-10)

2010

Paine, M.F. et al. (2010) Diamidines for human African trypanosomiasis. Current Opinion in Investigational Drugs, 11(8), pp. 876-883.

Berg, M. et al. (2010) Evaluation of nucleoside hydrolase inhibitors for treatment of African trypanosomiasis. Antimicrobial Agents and Chemotherapy, 54(5), pp. 1900-1908. (doi:10.1128/AAC.01787-09)

Changtam, C., de Koning, H.P., Ibrahim, H., Sajid, M.S., Gould, M.K., and Suksamrarn, A. (2010) Curcuminoid analogs with potent activity against Trypanosoma and Leishmania species. European Journal of Medicinal Chemistry, 45(3), pp. 941-956. (doi:10.1016/j.ejmech.2009.11.035)

Stewart, M.L. et al. (2010) Multiple genetic mechanisms lead to loss of functional TbAT1 expression in drug-resistant trypanosomes. Eukaryotic Cell, 9(2), pp. 336-343.

Altink, R., van Arkel, B., van der Baan, J.L., Balt, S., De Bolster, M.W.G., van Delft, R.J., Klumpp, G.W., De Koning, H., and van den Winkel, Y. (2010) A new model for coenzyme B12 based on a Costa-type ligand. Recueil des Travaux Chimiques des Pays-Bas, 113(6), pp. 329-333. (doi:10.1002/recl.19941130604)

Quashie, N.B., Ranford-Cartwright, L.C., and De Koning, H.P. (2010) Uptake of purines in Plasmodium falciparum-infected human erythrocytes is mostly mediated by the human Equilibrative Nucleoside Transporter and the human Facilitative Nucleobase Transporter. Malaria Journal, 9(36), (doi:10.1186/1475-2875-9-36)

Ward, C. P., Burgess, K. E.V., Burchmore, R.J.S., Barrett, M.P., and De Koning, H.P. (2010) A fluorescence-based assay for the uptake of CPD0801 (DB829) by African trypanosomes. Molecular and Biochemical Parasitology, 174(2), pp. 145-149. (doi:10.1016/j.molbiopara.2010.07.002)

2009

Kazibwe, A.J.N., Nerima, B., De Koning, H.P., Mäser, P., Barrett, M.P., and Matovu, E. (2009) Genotypic status of the TbAT1/P2 adenosine transporter of Trypanosoma brucei gambiense isolates from northwestern Uganda following melarsoprol withdrawal. PLoS Neglected Tropical Diseases, 3(9), e523. (doi:10.1371/journal.pntd.0000523)

Sanderson, L., Dogruel, M., Rodgers, J., De Koning, H.P., and Thomas, S.A. (2009) Pentamidine Movement across the Murine Blood-Brain and Blood-Cerebrospinal Fluid Barriers: Effect of Trypanosome Infection, Combination Therapy, P-Glycoprotein, and Multidrug Resistance-Associated Protein. Journal of Pharmacology and Experimental Therapeutics, 329(3), pp. 967-977. (doi:10.1124/jpet.108.149872)

Collar, C.J., Al Salabi, M.I., Stewart, M.L., Barrett, M.P., Wilson, W.D., and de Koning, H.P. (2009) Predictive computational models of substrate binding by a nucleoside transporter. Journal of Biological Chemistry, 284(49), pp. 34028-34035. (doi:10.1074/jbc.M109.049726)

2008

Gould, M.K., Vu, X.L., Seebeck, T., and De Koning, H.P. (2008) Propidium iodide-based methods for monitoring drug action in the kinetoplastidae: Comparison with the Alamar Blue assay. Analytical Biochemistry, 382(2), pp. 87-93. (doi:10.1016/j.ab.2008.07.036)

Quashie, N.B., Dorin-Semblat, D., Bray, P.G., Biagini, G.A., Doerig, C., Ranford-Cartwright, L.C., and De Koning, H.P. (2008) A comprehensive model of purine uptake by the malaria parasite Plasmodium falciparum: identification of four purine transport activities in intraerythrocytic parasites. Biochemical Journal, 411(2), pp. 287-295. (doi:10.1042/BJ20071460)

De Koning, H.P. (2008) Ever-increasing complexities of diamidine and arsenical crossresistance in African trypanosomes. Trends in Parasitology, 24(8), pp. 345-349. (doi:10.1016/j.pt.2008.04.006)

Papageorgiou, I., De Koning, H.P., Soteriadou, K., and Diallinas, G. (2008) Kinetic and mutational analysis of the Trypanosoma brucei NBT1 nucleobase transporter expressed in Saccharomyces cerevisiae reveals structural similarities between ENT and MFS transporters. International Journal for Parasitology, 38(6), pp. 641-653. (doi:10.1016/j.ijpara.2007.10.005)

2007

De Koning, H. P. (2007) Pyrimidine transporters of trypanosomes: a class apart? Trends in Parasitology, 23(5), p. 190. (doi:10.1016/j.pt.2007.03.004)

Bridges, D. J., Gould, M. K., Nerima, B., Maser, P., Burchmore, R. J. S., and de Koning, H. P. (2007) Loss of the high-affinity pentamidine transporter is responsible for high levels of cross-resistance between arsenical and diamidine drugs in African trypanosomes. Molecular Pharmacology, 71, pp. 1098-1108. (doi:10.1124/mol.106.031351)

Al-Salabi, M., Wallace, L., Luscher, A., Maser, P., Candlish, D., Rodenko, B., Gould, M., Jabeen, I., Ajith, S., and de Koning, H. (2007) Molecular interactions underlying the unusually high adenosine affinity of a novel Trypanosoma brucei nucleoside transporter. Molecular Pharmacology, 71, pp. 921-929. (doi:10.1124/mol.106.031559)

Delespaux, V., and de Koning, H. (2007) Drugs and drug resistance in African trypanosomiasis. Drug Resistance Updates, 10, pp. 30-50. (doi:10.1016/j.drup.2007.02.004)

Luscher, A., de Koning, H., and Maser, P. (2007) Chemotherapeutic strategies against Trypanosoma brucei: Drug targets vs. drug targeting. Current Pharmaceutical Design, 13, pp. 555-567.

2006

Anene, B.M., Ezeokonkwo, R.C., Mmesirionye, T.I., Tettey, J.N.A., Brock, J.M., Barrett, M.P., and De Koning, H.P. (2006) A diminazene-resistant strain of Trypanosoma brucei brucei isolated from a dog is cross-resistant to pentamidine in experimentally infected albino rats. Parasitology, 132(1), pp. 127-133. (doi:10.1017/S0031182005008760)

Gudin, S., Quashie, N., Candlish, D., Al-Salabi, M., Jarvis, S., Ranford-Cartwright, L., and de Koning, H. (2006) Trypanosoma brucei: A survey of pyrimidine transport activities. Experimental Parasitology, 114, pp. 118-125. (doi:10.1016/j.expara.2006.02.018)

Stoffel, S., Rodenko, B., Schweingruber, A., Maser, P., de Koning, H., and Schweingruber, M. (2006) Biosynthesis and uptake of thiamine (vitamin B-1) in bloodstream form Trypanosoma brucei brucei and interference of the vitamin with melarsen oxide activity. International Journal For Parasitology, 36, pp. 229-236. (doi:10.1016/j.ijpara.2005.10.003)

2005

De Koning, H. P., Bridges, D. J., and Burchmore, R. J. S. (2005) Purine and pyrimidine transport in pathogenic protozoa: From biology to therapy. FEMS Microbiology Reviews, 29(5), pp. 987-1020. (doi:10.1016/j.femsre.2005.03.004)

Geiser, F., Lüscher, A., De Koning, H.P., Seebeck, T., and Mäser, P. (2005) Molecular pharmacology of adenosine transport in trypanosoma brucei: P1/P2 revisited. Molecular Pharmacology, 68(3), pp. 589-595. (doi:10.1124/mol.104.010298)

Natto, M.J., Wallace, L.J.M., Candlish, D., Al-Salabi, M.I., Coutts, S.E., and de Koning, H.P. (2005) Trypanosoma brucei: expression of multiple purine transporters prevents the development of allopurinol resistance. Experimental Parasitology, 109(2), pp. 80-86. (doi:10.1016/j.exppara.2004.11.004)

Al-Salabi, M., and de Koning, H. (2005) Purine nucleobase transport in amastigotes of Leishmania mexicana: Involvement in allopurinol uptake. Antimicrobial Agents and Chemotherapy, 49, pp. 3682-3689. (doi:10.1128/AAC.49.9.3682-3689.2005)

Papageorgiou, I., Yakob, L., Al Salabi, M., Diallinas, G., Soteriadou, K., and De Koning, H. (2005) Identification of the first pyrimidine nucleobase transporter in Leishmania: similarities with the Trypanosoma brucei U1 transporter and antileishmanial activity of uracil analogues. Parasitology, 130, pp. 275-283. (doi:10.1017/S0031182004006626)

Stewart, M., Krishna, S., Burchmore, R., Brun, R., de Koning, H., Boykin, D., Tidwell, R., Hall, J., and Barrett, M. (2005) Detection of arsenical drug resistance in Trypanosoma brucei with a simple fluorescence test. Lancet, 366, pp. 486-487. (doi:10.1016/S0140-6736(05)66793-1)

2004

Biagini, G., Pasini, E., Hughes, R., De Koning, H., Vial, H., ONeill, P., Ward, S., and Bray, P. (2004) Characterization of the choline carrier of Plasmodium falciparum: a route for the selective delivery of novel antimalarial chugs. Blood, 104, pp. 3372-3377.

Wallace, L., Candlish, D., Hagos, A., Seley, K., and de Koning, H. (2004) Selective transport of a new class of purine antimetabolites by the protozoan parasite Trypanosoma brucei. Nucleosides Nucleotides and Nucleic Acids, 23, pp. 1441-1444. (doi:10.1081/NCN-200027660)

de Koning, H., Anderson, L., Stewart, M., Burchmore, R., Wallace, L., and Barrett, M. (2004) The trypanocide diminazene aceturate is accumulated predominantly through the TbAT1 purine transporter: additional insights on diamidine resistance in African trypanosomes. Antimicrobial Agents and Chemotherapy, 48, pp. 1515-1519. (doi:10.1128/AAC.48.5.1515-1519.2004)

2003

De Koning, H., Al-Salabi, M., Cohen, A., Coombs, G., and Wastling, J. (2003) Identification and characterisation of high affinity nucleoside and nucleobase transporters in Toxoplasma gondii. International Journal for Parasitology, 33(8), pp. 821-831. (doi:10.1016/S0020-7519(03)00091-2)

Al-Salabi, M., Wallace, L., and De Koning, H. (2003) A Leishmania major nucleobase transporter responsible for allopurinol uptake is a functional homolog of the Trypanosoma brucei H2 transporter. Molecular Pharmacology, 63, pp. 814-820.

Bray, P., Barrett, M., Ward, S., and de Koning, H. (2003) Pentamidine uptake and resistance in pathogenic protozoa: past, present and future. Trends in Parasitology, 19(5), pp. 232-239. (doi:10.1016/S1471-4922(03)00069-2)

Burchmore, R., Wallace, L., Candlish, D., Al-Salabi, M., Beal, P., Barrett, M., Baldwin, S., and de Koning, H. (2003) Cloning, heterologous expression, and in situ characterization of the first high affinity nucleobase transporter from a protozoan. Journal of Biological Chemistry, 278(26), pp. 23502-23507. (doi:10.1074/jbc.M301252200)

Matovu, E. et al. (2003) Mechanisms of arsenical and diamidine uptake and resistance in Trypanosoma brucei. Eukaryotic Cell, 2(5), pp. 1003-1008. (doi:10.1128/EC.2.5.1003-1008.2003)

2002

Wallace, L., Candlish, D., and De Koning, H. (2002) Different substrate recognition motifs of human and trypanosome nucleobase transporters - Selective uptake of purine antimetabolites. Journal of Biological Chemistry, 277, pp. 26149-26156. (doi:10.1074/jbc.M202835200)

2001

Stead, A.M.W., Bray, P.G., Edwards, I.G., De Koning, H.P., Elford, B.C., Stocks, P.A., and Ward, S. (2001) Diamidine compounds: Selective uptake and targeting in Plasmodium falciparum. Molecular Pharmacology, 59(5), pp. 1298-1306.

De Koning, H. (2001) Transporters in African trypanosomes: role in drug action and resistance. International Journal for Parasitology, 31(5-6), pp. 511-521. (doi:10.1016/S0020-7519(01)00167-9)

de Koning, H. (2001) Uptake of pentamidine in Trypanosoma brucei brucei is mediated by three distinct transporters: Implications for cross-resistance with arsenicals. Molecular Pharmacology, 59, pp. 586-592.

de Koning, H., and Jarvis, S. (2001) Uptake of pentamidine in Trypanosoma brucei brucei is mediated by the P2 adenosine transporter and at least one novel, unrelated transporter. Acta Tropica, 80, pp. 245-250.

2000

De Koning, H., and Diallinas, G. (2000) Nucleobase transporters: review. Molecular Membrane Biology, 17(2), pp. 75-94. (doi:10.1080/09687680050117101)

De Koning, H.P., MacLeod, A., Barrett, M.P., Cover, B., and Jarvis, S.M. (2000) Further evidence for a link between melarsoprol resistance and P2 transporter function in African trypanosomes. Molecular and Biochemical Parasitology, 106(1), pp. 181-185. (doi:10.1016/S0166-6851(99)00206-6)

De Koning, H.P., Watson, C.J., Sutcliffe, L., and Jarvis, S.M. (2000) Differential regulation of nucleoside and nucleobase transporters in Crithidia fasciculata and Trypanosoma brucei brucei. Molecular and Biochemical Parasitology, 106(1), pp. 93-107. (doi:10.1016/S0166-6851(99)00203-0)

1999

Carter, N.S., Barrett, M.P., and De Koning, H.P. (1999) A drug resistance determinant in trypanosoma brucei. Trends in Microbiology, 7(12), pp. 469-471. (doi:10.1016/S0966-842X(99)01643-1)

De Koning, H.P., and Jarvis, S.M. (1999) Adenosine transporters in bloodstream forms of trypanosoma brucei brucei: substrate recognition motifs and affinity for trypanocidal drugs. Molecular Pharmacology, 56(6), pp. 1162-1170.

1998

De Koning, H.P., and Jarvis, S.M. (1998) A highly selective, high-affinity transporter for uracil in trypanosoma brucei brucei: evidence for proton-dependent transport. Biochemistry and Cell Biology, 76(5), pp. 853-858. (doi:10.1139/o98-086)

Jenkd, B., Buzzi, M., Dotman, C., De Koning, H., Scheenen, W., Lisete, J., Leenders, H., Cruijsen, P., and Roubos, E. (1998) The significance of multiple inhibitory mechanisms converging on the melanotrope cell of Xenopus laevisa. Annals of the New York Academy of Sciences, 839, pp. 229-234. (doi:10.1111/j.1749-6632.1998.tb10765.x)

De Koning, H.P., Watson, C.J., and Jarvis, S.M. (1998) Characterization of a nucleoside/proton symporter in procyclic trypanosoma brucei brucei. Journal of Biological Chemistry, 273(16), pp. 9486-9494. (doi:10.1074/jbc.273.16.9486)

1997

De Koning, H.P., and Jarvis, S.M. (1997) Purine nucleobase transport in bloodstream forms of trypanosoma brucei brucei is mediated by two novel transporters. Molecular and Biochemical Parasitology, 89(2), pp. 245-258. (doi:10.1016/S0166-6851(97)00129-1)

De Koning, H.P., and Jarvis, S.M. (1997) Hypoxanthine iptake through a purine-selective nucleobase transporter in trypano soma brucei brucei procyclic cells is driven by protonmotive force. European Journal of Biochemistry, 247(3), pp. 1102-1110. (doi:10.1111/j.1432-1033.1997.01102.x)

De Koning, H. P., and Jarvis, S. M. (1997) Purine nucleobase transport in bloodstream forms of Trypanosoma brucei brucei. Biochemical Society Transactions, 25, 476S. (doi:10.1042/bst025476s)

1994

Scheenen, W. J.J.M., De Koning, H. P., Jenks, B. G., Vaudry, H., and Roubos, E. W. (1994) The secretion of a-MSH from Xenopus melanotropes involves calcium influx through m-conotoxin-sensitive voltage-operated calcium channels. Journal of Neuroendocrinology, 6(4), pp. 457-464. (doi:10.1111/j.1365-2826.1994.tb00607.x)

1993

De Koning, H.P., Jenks, B.G., and Roubos, E.W. (1993) Analysis of gamma-aminobutyric acidB receptor function in the in vitro and in vivo regulation of alpha-melanotropin-stimulating hormone secretion from melanotrope cells of Xenopus laevis. Endocrinology, 132(2), pp. 674-681. (doi:10.1210/endo.132.2.8381070)

Jenks, B.G., De Koning, H.P., Cruisjen, P.M.J.M., Mauger, C.M.A., Roubos, E.W., Tonon, M.C., Desrues, L., and Vaudry, H. (1993) Analysis of inositol phosphate metabolism in melanotrope cells of Xenopus laevis in relation to background adaptation. Annals of the New York Academy of Sciences, 680, pp. 188-198. (doi:10.1111/j.1749-6632.1993.tb19684.x)

Jenks, B. G., De Koning, H. P., Valentijn, K., and Roubos, E. W. (1993) Dual action of GABAA receptors on the secretory process of melanotrophs of Xenopus laevis. Neuroendocrinology, 58(1), pp. 80-85. (doi:10.1159/000126516)

Leenders, H.J., De Koning, H.P., Ponten, S.P., Jenks, B.G., and Roubos, E.W. (1993) Differential effects of coexisting dopamine, GABA and NPY on α-MSH secretion from melanotrope cells of Xenopus laevis. Life Sciences, 52(24), pp. 1969-1975. (doi:10.1016/0024-3205(93)90638-J)

1992

De Koning, H. P./, Jenks, B. G., Scheenen, W. J.J.M., Balm, P. H.M., and Roubos, E. W. (1992) Analysis of autofeedback mechanisms in the secretion of pro-opiomelanocortin-derived peptides by melanotrope cells of Xenopus laevis. General and Comparative Endocrinology, 87(3), pp. 394-401. (doi:10.1016/0016-6480(92)90046-M)

De Koning, H. P. (1992) Receptors and Signal Transduction in the Pars Intermedia of Xenopus Laevis. Krips Repro. ISBN 9789090054001

De Koning, H. P., Jenks, B. G., Huchede, B., and Roubos, E. W. (1992) Dynamics of cyclic-AMP efflux in relation to α-MSH secretion from melanotrope cells of Xenopus laevis. Life Sciences, 51(21), pp. 1667-1673. (doi:10.1016/0024-3205(92)90311-C)

1991

De Koning, H. P., Jenks, B. G., Scheenen, W. J.J.M., de Rijk, E. P.C.T., Cans, R. T.J.M., and Roubos, E. W. (1991) Indirect action of elevated potassium and neuropeptide y on αMSH secretion from the pars intermedia of Xenopus laevi: a biochemical and morphological study. Neuroendocrinology, 54(1), pp. 68-76. (doi:10.1159/000125853)

Jenks, B.G., van Zoest, I.D., De Koning, H.P., Leenders, H.J., and Roubos, E.W. (1991) The CRF-related peptide sauvagine stimulates and the GABAB receptor agonist baclofen inhibits cyclic-AMP production in melanotrope cells of Xenopus laevis. Life Sciences, 48(17), pp. 1633-1637. (doi:10.1016/0024-3205(91)90123-S)

1988

van Arkel, B., van der Baan, J.L., Balt, S., de Bolster, M. W.G., van Delft, R.J., Klumpp, G.W., De Koning, H., and van den Winkel, Y. (1988) A new model for coenzyme B12. Recueil des Travaux Chimiques des Pays-Bas, 107(1), pp. 23-24. (doi:10.1002/recl.19881070106)

This list was generated on Sun Jul 24 15:45:24 2016 BST.
Number of items: 108.

Articles

Vodnala, M., Ranjbarian, F., Pavlova, A., de Koning, H. P., and Hofer, A. (2016) Trypanosoma brucei methylthioadenosine phosphorylase protects the parasite from the antitrypanosomal effect of deoxyadenosine. Journal of Biological Chemistry, 291(22), pp. 11717-11726. (doi:10.1074/jbc.M116.715615) (PMID:27036940)

Dike, V. T., Vihiior, B., Bosha, J. A., Yin, T. M., Ebiloma, G. U., de Koning, H. P., Igoli, J. O., and Gray, A. I. (2016) Antitrypanosomal activity of a novel taccalonolide from the tubers of Tacca leontopetaloides. Phytochemical Analysis, 27(3-4), pp. 217-221. (doi:10.1002/pca.2619) (PMID:27313159)

Imamura, H. et al. (2016) Evolutionary genomics of epidemic visceral leishmaniasis in the Indian subcontinent. eLife, 5, e12613. (doi:10.7554/eLife.12613) (PMID:27003289) (PMCID:PMC4811772)

Graf, F. E. et al. (2016) Comparative genomics of drug resistance in Trypanosoma brucei rhodesiense. Cellular and Molecular Life Sciences, (doi:10.1007/s00018-016-2173-6) (PMID:26973180) (Early Online Publication)

Omar, R. M.K., Igoli, J., Gray, A. I., Ebiloma, G. U., Clements, C., Fearnley, J., Edrada Ebel, R. A., Zhang, T., De Koning, H., and Watson, D. (2016) Chemical characterisation of Nigerian red propolis and its biological activity against Trypanosoma Brucei. Phytochemical Analysis, 27(2), pp. 107-115. (doi:10.1002/pca.2605) (PMID:26662866)

Babuadze, G. et al. (2016) Seroepidemiology and molecular diversity of Leishmania donovani complex in Georgia. Parasites and Vectors, 9, 279. (doi:10.1186/s13071-016-1558-6) (PMID:27177688) (PMCID:PMC4866401)

Siheri, W. et al. (2016) Chemical and antimicrobial profiling of propolis from different regions within Libya. PLoS ONE, 11(5), e0155355. (doi:10.1371/journal.pone.0155355) (PMID:27195790) (PMCID:PMC4873177)

Alkhaldi, A. A.M., Martinek, J., Panicucci, B., Dardonville, C., Zikova, A., and De Koning, H. (2015) Trypanocidal action of bisphosphonium salts through a mitochondrial target in bloodstream form Trypanosoma brucei. International Journal for Parasitology: Drugs and Drug Resistance, 6(1), pp. 23-34. (doi:10.1016/j.ijpddr.2015.12.002)

Rodenko, B., Wanner, M., Alkhaldi, A., Ebiloma, G., Barnes, R., Kaiser, M., Brun, R., McCulloch, R., Koomen, G.-J., and De Koning, H. P. (2015) Targeting the parasite's DNA with methyltriazenyl purine analogs Is a safe, selective, and efficacious antitrypanosomal strategy. Antimicrobial Agents and Chemotherapy, 59(11), pp. 6708-6716. (doi:10.1128/aac.00596-15) (PMID:26282430) (PMCID:PMC4604408)

Ríos Martínez, C. H., Nué Martínez, J. J., Ebiloma, G. U., De Koning, H. P., Alkorta, I., and Dardonville, C. (2015) Lowering the pKa of a bisimidazoline lead with halogen atoms results in improved activity and selectivity against Trypanosoma brucei in vitro. European Journal of Medicinal Chemistry, 101, pp. 806-817. (doi:10.1016/j.ejmech.2015.07.013) (PMID:26231081)

Graf, F. E., Baker, N., Munday, J. C., De Koning, H. P., Horn, D., and Mäser, P. (2015) Chimerization at the AQP2–AQP3 locus is the genetic basis of melarsoprol–pentamidine cross-resistance in clinical Trypanosoma brucei gambiense isolates. International Journal for Parasitology: Drugs and Drug Resistance, 5(2), pp. 65-68. (doi:10.1016/j.ijpddr.2015.04.002) (PMID:26042196) (PMCID:PMC4443405)

Munday, J. C. et al. (2015) Functional analysis of drug resistance-associated mutations in the Trypanosoma brucei Adenosine Transporter 1 (TbAT1) and the proposal of a structural model for the protein. Molecular Microbiology, 96(4), pp. 887-900. (doi:10.1111/mmi.12979) (PMID:25708978)

Alkhaldi, A.A.M., Creek, D.J., Ibrahim, H., Kim, D.-H., Quashie, N.B., Burgess, K.E., Changtam, C., Barrett, M.P., Suksamrarn, A., and De Koning, H.P. (2015) Potent Trypanocidal curcumin analogs bearing a monoenone linker motif act on Trypanosoma brucei by forming an adduct with Trypanothione. Molecular Pharmacology, 87(3), pp. 451-464. (doi:10.1124/mol.114.096016)

Ríos Martínez, C. H., Miller, F., Ganeshamoorthy, K., Glacial, F., Kaiser, M., De Koning, H., Eze, A. A., Lagartera, L., Herraiz, T., and Dardonville, C. (2015) A new nonpolar N-hydroxy imidazoline lead compound with improved activity in a murine model of late-stage Trypanosoma brucei brucei infection is not cross-resistant with diamidines. Antimicrobial Agents and Chemotherapy, 59(2), pp. 890-904. (doi:10.1128/AAC.03958-14)

Shameer, S. et al. (2015) TrypanoCyc: a community-led biochemical pathways database for Trypanosoma brucei. Nucleic Acids Research, 43(D1), D637-D644. (doi:10.1093/nar/gku944)

Dardonville, C., Alkhaldi, A. A.M., and De Koning, H. P. (2015) SAR studies of diphenyl cationic trypanocides: superior activity of phosphonium over ammonium salts. ACS Medicinal Chemistry Letters, 6(2), pp. 151-155. (doi:10.1021/ml500408d)

Munday, J. C., Settimo, L., and De Koning, H. P. (2015) Transport proteins determine drug sensitivity and resistance in a protozoan parasite, Trypanosoma brucei. Frontiers in Pharmacology, 6, 32. (doi:10.3389/fphar.2015.00032) (PMID:24235095) (PMCID:PMC3922157)

Natto, M. J., Eze, A. A., and De Koning, H. P. (2015) Protocols for the routine screening of drug sensitivity in the human parasite Trichomonas vaginalis. Methods in Molecular Biology, 1263, pp. 103-110. (doi:10.1007/978-1-4939-2269-7_8)

Tagoe, D. N. A., Kalejaiye, T. D., and De Koning, H. P. (2015) The ever unfolding story of cAMP signaling in trypanosomatids: vive la difference! Frontiers in Pharmacology, 6, 185. (doi:10.3389/fphar.2015.00185) (PMID:26441645) (PMCID:PMC4561360)

Unciti-Broceta, J. D., Arias, J. L., Maceira, J., Soriano, M., Ortiz-González, M., Hernández-Quero, J., Muñóz-Torres, M., De Koning, H. P., Magez, S., and Garcia-Salcedo, J. A. (2015) Specific cell targeting therapy bypasses drug resistance mechanisms in African trypanosomiasis. PLoS Pathogens, 11(6), e1004942. (doi:10.1371/journal.ppat.1004942) (PMID:26110623) (PMCID:PMC4482409)

Acosta-Reyes, F. J., Dardonville, C., De Koning, H. P., Natto, M., Subirana, J. A., and Campos, J. L. (2014) In and out of the minor groove: interaction of an AT-rich DNA with the drug CD27. Acta Crystallographica. Section D: Biological Crystallography, 70(6), pp. 1614-1621. (doi:10.1107/S139900471400697X)

Lüscher, A., Lamprea-Burgunder, E., Graf, F. E., De Koning, H., and Mäser, P. (2014) Trypanosoma brucei adenine-phosphoribosyltransferases mediate adenine salvage and aminopurinol susceptibility but not adenine toxicity. International Journal for Parasitology: Drugs and Drug Resistance, 4(1), pp. 55-63. (doi:10.1016/j.ijpddr.2013.12.001)

Munday, J. C. et al. (2014) Trypanosoma brucei aquaglyceroporin 2 is a high-affinity transporter for pentamidine and melaminophenyl arsenic drugs and the main genetic determinant of resistance to these drugs. Journal of Antimicrobial Chemotherapy, 69(3), pp. 651-663. (doi:10.1093/jac/dkt442) (PMID:24235095) (PMCID:PMC3922157)

Babuadze, G. et al. (2014) Epidemiology of Visceral Leishmaniasis in Georgia. PLoS Neglected Tropical Diseases, 8(3), e2725. (doi:10.1371/journal.pntd.0002725)

Zimmermann, S. C., O'Neill, E., Ebiloma, G. U., Wallace, L. J.M., De Koning, H. P., and Seley-Radtke, K. L. (2014) Design and synthesis of a series of truncated neplanocin fleximers. Molecules, 19(12), pp. 21200-21214. (doi:10.3390/molecules191221200)

Munday, J.C., Rojas López, K.E., Eze, A.A., Delespaux, V., Van Den Abbeele, J., Rowan, T., Barrett, M.P., Morrison, L.J., and De Koning, H.P. (2013) Functional expression of TcoAT1 reveals it to be a P1-type nucleoside transporter with no capacity for diminazene uptake. International Journal for Parasitology: Drugs and Drug Resistance, 3, pp. 69-76. (doi:10.1016/j.ijpddr.2013.01.004)

Vodnala, S.K. et al. (2013) Structure–activity relationships of synthetic cordycepin analogues as experimental therapeutics for African trypanosomiasis. Journal of Medicinal Chemistry, 56(24), pp. 9861-9873. (doi:10.1021/jm401530a)

Graf, F. E., Wenzler, T., Ludin, P., Kaiser, M., Brun, R., Pyana, P. P., Buscher, P., De Koning, H. P., Horn, D., and Maser, P. (2013) Aquaporin 2 mutations in Trypanosoma brucei gambiense field isolates correlate with decreased susceptibility to pentamidine and melarsoprol. PLoS Neglected Tropical Diseases, 7(10), e2475. (doi:10.1371/journal.pntd.0002475)

Gould, M.K., Bachmaier, S., Ali, J.A.M., Alsford, S., Tagoe, D.N.A., Munday, J.C., Schnaufer, A.C., Horn, D., Boshart, M., and De Koning, H. (2013) Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNA interference library screening for resistance to the phosphodiesterase inhibitor CpdA. Antimicrobial Agents and Chemotherapy, 57(10), pp. 4882-4893. (doi:10.1128/AAC.00508-13)

Baker, N., De Koning, H.P., Mäser, P., and Horn, D. (2013) Drug resistance in African trypanosomiasis: the melarsoprol and pentamidine story. Trends in Parasitology, 29(3), pp. 110-118. (doi:10.1016/j.pt.2012.12.005)

Ali, J.A.M., Creek, D.J., Burgess, K., Allison, H.C., Field, M.C., Maser, P., and De Koning, H.P. (2013) Pyrimidine salvage in trypanosoma brucei bloodstream forms and the trypanocidal action of halogenated pyrimidines. Molecular Pharmacology, 83(2), pp. 439-453. (doi:10.1124/mol.112.082321) (PMID:23188714)

Ali, J. A.., Tagoe, D. N.A., Munday, J. C., Donachie, A., Morrison, L. J., and De Koning, H. P. (2013) Pyrimidine biosynthesis is not an essential function for trypanosoma brucei bloodstream forms. PLoS ONE, 8(3), e58034. (doi:10.1371/journal.pone.0058034) (PMID:23505454) (PMCID:PMC3591441)

Baker, N., Glover, L., Munday, J. C., Aguinaga Andres, D., Barrett, M. P., De Koning, H. P., and Horn, D. (2012) Aquaglyceroporin 2 controls susceptibility to melarsoprol and pentamidine in African trypanosomes. Proceedings of the National Academy of Sciences of the United States of America, 109(27), pp. 10996-11001. (doi:10.1073/pnas.1202885109)

De Koning, H.P., Gould, M.K., Sterk, G.J., Tenor, H., Kunz, S., Luginbuehl, E., and Seebeck, T. (2012) Pharmacological validation of Trypanosoma brucei phosphodiesterases as novel drug targets. Journal of Infectious Diseases, 206(2), pp. 229-237. (doi:10.1093/infdis/jir857)

Natto, M.J., Savioli, F., Quashie, N.B., Dardonville, C., Rodenko, B., and De Koning, H.P. (2012) Validation of novel fluorescence assays for the routine screening of drug susceptibilities of Trichomonas vaginalis. Journal of Antimicrobial Chemotherapy, 67(4), pp. 933-943. (doi:10.1093/jac/dkr572)

Taladriz, A. et al. (2012) Synthesis and structure–activity analysis of new phosphonium salts with potent activity against African Trypanosomes. Journal of Medicinal Chemistry, 55(6), pp. 2606-2622. (doi:10.1021/jm2014259)

Teka, I. A. et al. (2011) The diamidine diminazene aceturate is a substrate for the high-affinity pentamidine transporter: implications for the development of high resistance levels in trypanosomes. Molecular Pharmacology, 80(1), pp. 110-116. (doi:10.1124/mol.111.071555)

Ibrahim, H.M.S., Al-Salabi, M.I., El Sabbagh, N., Quashie, N.B., Alkhaldi, A.A.M., Escale, R., Smith, T.K., Vial, H.J., and De Koning, H.P. (2011) Symmetrical choline-derived dications display strong anti-kinetoplastid activity. Journal of Antimicrobial Chemotherapy, 66(1), pp. 111-125. (doi:10.1093/jac/dkq401)

Gould, M.K., and De Koning, H.P. (2011) Cyclic-nucleotide signalling in protozoa. FEMS Microbiology Reviews, 31(3), pp. 515-541. (doi:10.1111/j.1574-6976.2010.00262.x)

Rodenko, B., Al-Salabi, M.I., Teka, I.A., Ho, W., El-Sabbagh, N., Ali, J.A.M., Ibrahim, H.M.S., Wanner, M.J., Koomen, G.-J., and De Koning, H.P. (2011) Synthesis of marine-derived 3-alkylpyridinium alkaloids with potent antiprotozoal activity. ACS Medicinal Chemistry Letters, 2(12), pp. 901-906. (doi:10.1021/ml200160k)

Shakur, Y., De Koning, H.P., Ke, H., Kambayashi, J., and Seebeck, T. (2011) Therapeutic potential of phosphodiesterase inhibitors in parasitic diseases. Handbook of Experimental Pharmacology, 204, pp. 487-510. (doi:10.1007/978-3-642-17969-3_20)

Ward, C.P., Wong, P.E., Burchmore, R.J.S., De Koning, H.P., and Barrett, M.P. (2011) Trypanocidal furamidine analogues: influence of pyridine nitrogens on trypanocidal activity, transport kinetics, and resistance patterns. Antimicrobial Agents and Chemotherapy, 55(5), pp. 2352-2361. (doi:10.1128/AAC.01551-10)

Paine, M.F. et al. (2010) Diamidines for human African trypanosomiasis. Current Opinion in Investigational Drugs, 11(8), pp. 876-883.

Berg, M. et al. (2010) Evaluation of nucleoside hydrolase inhibitors for treatment of African trypanosomiasis. Antimicrobial Agents and Chemotherapy, 54(5), pp. 1900-1908. (doi:10.1128/AAC.01787-09)

Changtam, C., de Koning, H.P., Ibrahim, H., Sajid, M.S., Gould, M.K., and Suksamrarn, A. (2010) Curcuminoid analogs with potent activity against Trypanosoma and Leishmania species. European Journal of Medicinal Chemistry, 45(3), pp. 941-956. (doi:10.1016/j.ejmech.2009.11.035)

Stewart, M.L. et al. (2010) Multiple genetic mechanisms lead to loss of functional TbAT1 expression in drug-resistant trypanosomes. Eukaryotic Cell, 9(2), pp. 336-343.

Altink, R., van Arkel, B., van der Baan, J.L., Balt, S., De Bolster, M.W.G., van Delft, R.J., Klumpp, G.W., De Koning, H., and van den Winkel, Y. (2010) A new model for coenzyme B12 based on a Costa-type ligand. Recueil des Travaux Chimiques des Pays-Bas, 113(6), pp. 329-333. (doi:10.1002/recl.19941130604)

Quashie, N.B., Ranford-Cartwright, L.C., and De Koning, H.P. (2010) Uptake of purines in Plasmodium falciparum-infected human erythrocytes is mostly mediated by the human Equilibrative Nucleoside Transporter and the human Facilitative Nucleobase Transporter. Malaria Journal, 9(36), (doi:10.1186/1475-2875-9-36)

Ward, C. P., Burgess, K. E.V., Burchmore, R.J.S., Barrett, M.P., and De Koning, H.P. (2010) A fluorescence-based assay for the uptake of CPD0801 (DB829) by African trypanosomes. Molecular and Biochemical Parasitology, 174(2), pp. 145-149. (doi:10.1016/j.molbiopara.2010.07.002)

Kazibwe, A.J.N., Nerima, B., De Koning, H.P., Mäser, P., Barrett, M.P., and Matovu, E. (2009) Genotypic status of the TbAT1/P2 adenosine transporter of Trypanosoma brucei gambiense isolates from northwestern Uganda following melarsoprol withdrawal. PLoS Neglected Tropical Diseases, 3(9), e523. (doi:10.1371/journal.pntd.0000523)

Sanderson, L., Dogruel, M., Rodgers, J., De Koning, H.P., and Thomas, S.A. (2009) Pentamidine Movement across the Murine Blood-Brain and Blood-Cerebrospinal Fluid Barriers: Effect of Trypanosome Infection, Combination Therapy, P-Glycoprotein, and Multidrug Resistance-Associated Protein. Journal of Pharmacology and Experimental Therapeutics, 329(3), pp. 967-977. (doi:10.1124/jpet.108.149872)

Collar, C.J., Al Salabi, M.I., Stewart, M.L., Barrett, M.P., Wilson, W.D., and de Koning, H.P. (2009) Predictive computational models of substrate binding by a nucleoside transporter. Journal of Biological Chemistry, 284(49), pp. 34028-34035. (doi:10.1074/jbc.M109.049726)

Gould, M.K., Vu, X.L., Seebeck, T., and De Koning, H.P. (2008) Propidium iodide-based methods for monitoring drug action in the kinetoplastidae: Comparison with the Alamar Blue assay. Analytical Biochemistry, 382(2), pp. 87-93. (doi:10.1016/j.ab.2008.07.036)

Quashie, N.B., Dorin-Semblat, D., Bray, P.G., Biagini, G.A., Doerig, C., Ranford-Cartwright, L.C., and De Koning, H.P. (2008) A comprehensive model of purine uptake by the malaria parasite Plasmodium falciparum: identification of four purine transport activities in intraerythrocytic parasites. Biochemical Journal, 411(2), pp. 287-295. (doi:10.1042/BJ20071460)

De Koning, H.P. (2008) Ever-increasing complexities of diamidine and arsenical crossresistance in African trypanosomes. Trends in Parasitology, 24(8), pp. 345-349. (doi:10.1016/j.pt.2008.04.006)

Papageorgiou, I., De Koning, H.P., Soteriadou, K., and Diallinas, G. (2008) Kinetic and mutational analysis of the Trypanosoma brucei NBT1 nucleobase transporter expressed in Saccharomyces cerevisiae reveals structural similarities between ENT and MFS transporters. International Journal for Parasitology, 38(6), pp. 641-653. (doi:10.1016/j.ijpara.2007.10.005)

De Koning, H. P. (2007) Pyrimidine transporters of trypanosomes: a class apart? Trends in Parasitology, 23(5), p. 190. (doi:10.1016/j.pt.2007.03.004)

Bridges, D. J., Gould, M. K., Nerima, B., Maser, P., Burchmore, R. J. S., and de Koning, H. P. (2007) Loss of the high-affinity pentamidine transporter is responsible for high levels of cross-resistance between arsenical and diamidine drugs in African trypanosomes. Molecular Pharmacology, 71, pp. 1098-1108. (doi:10.1124/mol.106.031351)

Al-Salabi, M., Wallace, L., Luscher, A., Maser, P., Candlish, D., Rodenko, B., Gould, M., Jabeen, I., Ajith, S., and de Koning, H. (2007) Molecular interactions underlying the unusually high adenosine affinity of a novel Trypanosoma brucei nucleoside transporter. Molecular Pharmacology, 71, pp. 921-929. (doi:10.1124/mol.106.031559)

Delespaux, V., and de Koning, H. (2007) Drugs and drug resistance in African trypanosomiasis. Drug Resistance Updates, 10, pp. 30-50. (doi:10.1016/j.drup.2007.02.004)

Luscher, A., de Koning, H., and Maser, P. (2007) Chemotherapeutic strategies against Trypanosoma brucei: Drug targets vs. drug targeting. Current Pharmaceutical Design, 13, pp. 555-567.

Anene, B.M., Ezeokonkwo, R.C., Mmesirionye, T.I., Tettey, J.N.A., Brock, J.M., Barrett, M.P., and De Koning, H.P. (2006) A diminazene-resistant strain of Trypanosoma brucei brucei isolated from a dog is cross-resistant to pentamidine in experimentally infected albino rats. Parasitology, 132(1), pp. 127-133. (doi:10.1017/S0031182005008760)

Gudin, S., Quashie, N., Candlish, D., Al-Salabi, M., Jarvis, S., Ranford-Cartwright, L., and de Koning, H. (2006) Trypanosoma brucei: A survey of pyrimidine transport activities. Experimental Parasitology, 114, pp. 118-125. (doi:10.1016/j.expara.2006.02.018)

Stoffel, S., Rodenko, B., Schweingruber, A., Maser, P., de Koning, H., and Schweingruber, M. (2006) Biosynthesis and uptake of thiamine (vitamin B-1) in bloodstream form Trypanosoma brucei brucei and interference of the vitamin with melarsen oxide activity. International Journal For Parasitology, 36, pp. 229-236. (doi:10.1016/j.ijpara.2005.10.003)

De Koning, H. P., Bridges, D. J., and Burchmore, R. J. S. (2005) Purine and pyrimidine transport in pathogenic protozoa: From biology to therapy. FEMS Microbiology Reviews, 29(5), pp. 987-1020. (doi:10.1016/j.femsre.2005.03.004)

Geiser, F., Lüscher, A., De Koning, H.P., Seebeck, T., and Mäser, P. (2005) Molecular pharmacology of adenosine transport in trypanosoma brucei: P1/P2 revisited. Molecular Pharmacology, 68(3), pp. 589-595. (doi:10.1124/mol.104.010298)

Natto, M.J., Wallace, L.J.M., Candlish, D., Al-Salabi, M.I., Coutts, S.E., and de Koning, H.P. (2005) Trypanosoma brucei: expression of multiple purine transporters prevents the development of allopurinol resistance. Experimental Parasitology, 109(2), pp. 80-86. (doi:10.1016/j.exppara.2004.11.004)

Al-Salabi, M., and de Koning, H. (2005) Purine nucleobase transport in amastigotes of Leishmania mexicana: Involvement in allopurinol uptake. Antimicrobial Agents and Chemotherapy, 49, pp. 3682-3689. (doi:10.1128/AAC.49.9.3682-3689.2005)

Papageorgiou, I., Yakob, L., Al Salabi, M., Diallinas, G., Soteriadou, K., and De Koning, H. (2005) Identification of the first pyrimidine nucleobase transporter in Leishmania: similarities with the Trypanosoma brucei U1 transporter and antileishmanial activity of uracil analogues. Parasitology, 130, pp. 275-283. (doi:10.1017/S0031182004006626)

Stewart, M., Krishna, S., Burchmore, R., Brun, R., de Koning, H., Boykin, D., Tidwell, R., Hall, J., and Barrett, M. (2005) Detection of arsenical drug resistance in Trypanosoma brucei with a simple fluorescence test. Lancet, 366, pp. 486-487. (doi:10.1016/S0140-6736(05)66793-1)

Biagini, G., Pasini, E., Hughes, R., De Koning, H., Vial, H., ONeill, P., Ward, S., and Bray, P. (2004) Characterization of the choline carrier of Plasmodium falciparum: a route for the selective delivery of novel antimalarial chugs. Blood, 104, pp. 3372-3377.

Wallace, L., Candlish, D., Hagos, A., Seley, K., and de Koning, H. (2004) Selective transport of a new class of purine antimetabolites by the protozoan parasite Trypanosoma brucei. Nucleosides Nucleotides and Nucleic Acids, 23, pp. 1441-1444. (doi:10.1081/NCN-200027660)

de Koning, H., Anderson, L., Stewart, M., Burchmore, R., Wallace, L., and Barrett, M. (2004) The trypanocide diminazene aceturate is accumulated predominantly through the TbAT1 purine transporter: additional insights on diamidine resistance in African trypanosomes. Antimicrobial Agents and Chemotherapy, 48, pp. 1515-1519. (doi:10.1128/AAC.48.5.1515-1519.2004)

De Koning, H., Al-Salabi, M., Cohen, A., Coombs, G., and Wastling, J. (2003) Identification and characterisation of high affinity nucleoside and nucleobase transporters in Toxoplasma gondii. International Journal for Parasitology, 33(8), pp. 821-831. (doi:10.1016/S0020-7519(03)00091-2)

Al-Salabi, M., Wallace, L., and De Koning, H. (2003) A Leishmania major nucleobase transporter responsible for allopurinol uptake is a functional homolog of the Trypanosoma brucei H2 transporter. Molecular Pharmacology, 63, pp. 814-820.

Bray, P., Barrett, M., Ward, S., and de Koning, H. (2003) Pentamidine uptake and resistance in pathogenic protozoa: past, present and future. Trends in Parasitology, 19(5), pp. 232-239. (doi:10.1016/S1471-4922(03)00069-2)

Burchmore, R., Wallace, L., Candlish, D., Al-Salabi, M., Beal, P., Barrett, M., Baldwin, S., and de Koning, H. (2003) Cloning, heterologous expression, and in situ characterization of the first high affinity nucleobase transporter from a protozoan. Journal of Biological Chemistry, 278(26), pp. 23502-23507. (doi:10.1074/jbc.M301252200)

Matovu, E. et al. (2003) Mechanisms of arsenical and diamidine uptake and resistance in Trypanosoma brucei. Eukaryotic Cell, 2(5), pp. 1003-1008. (doi:10.1128/EC.2.5.1003-1008.2003)

Wallace, L., Candlish, D., and De Koning, H. (2002) Different substrate recognition motifs of human and trypanosome nucleobase transporters - Selective uptake of purine antimetabolites. Journal of Biological Chemistry, 277, pp. 26149-26156. (doi:10.1074/jbc.M202835200)

Stead, A.M.W., Bray, P.G., Edwards, I.G., De Koning, H.P., Elford, B.C., Stocks, P.A., and Ward, S. (2001) Diamidine compounds: Selective uptake and targeting in Plasmodium falciparum. Molecular Pharmacology, 59(5), pp. 1298-1306.

De Koning, H. (2001) Transporters in African trypanosomes: role in drug action and resistance. International Journal for Parasitology, 31(5-6), pp. 511-521. (doi:10.1016/S0020-7519(01)00167-9)

de Koning, H. (2001) Uptake of pentamidine in Trypanosoma brucei brucei is mediated by three distinct transporters: Implications for cross-resistance with arsenicals. Molecular Pharmacology, 59, pp. 586-592.

de Koning, H., and Jarvis, S. (2001) Uptake of pentamidine in Trypanosoma brucei brucei is mediated by the P2 adenosine transporter and at least one novel, unrelated transporter. Acta Tropica, 80, pp. 245-250.

De Koning, H., and Diallinas, G. (2000) Nucleobase transporters: review. Molecular Membrane Biology, 17(2), pp. 75-94. (doi:10.1080/09687680050117101)

De Koning, H.P., MacLeod, A., Barrett, M.P., Cover, B., and Jarvis, S.M. (2000) Further evidence for a link between melarsoprol resistance and P2 transporter function in African trypanosomes. Molecular and Biochemical Parasitology, 106(1), pp. 181-185. (doi:10.1016/S0166-6851(99)00206-6)

De Koning, H.P., Watson, C.J., Sutcliffe, L., and Jarvis, S.M. (2000) Differential regulation of nucleoside and nucleobase transporters in Crithidia fasciculata and Trypanosoma brucei brucei. Molecular and Biochemical Parasitology, 106(1), pp. 93-107. (doi:10.1016/S0166-6851(99)00203-0)

Carter, N.S., Barrett, M.P., and De Koning, H.P. (1999) A drug resistance determinant in trypanosoma brucei. Trends in Microbiology, 7(12), pp. 469-471. (doi:10.1016/S0966-842X(99)01643-1)

De Koning, H.P., and Jarvis, S.M. (1999) Adenosine transporters in bloodstream forms of trypanosoma brucei brucei: substrate recognition motifs and affinity for trypanocidal drugs. Molecular Pharmacology, 56(6), pp. 1162-1170.

De Koning, H.P., and Jarvis, S.M. (1998) A highly selective, high-affinity transporter for uracil in trypanosoma brucei brucei: evidence for proton-dependent transport. Biochemistry and Cell Biology, 76(5), pp. 853-858. (doi:10.1139/o98-086)

Jenkd, B., Buzzi, M., Dotman, C., De Koning, H., Scheenen, W., Lisete, J., Leenders, H., Cruijsen, P., and Roubos, E. (1998) The significance of multiple inhibitory mechanisms converging on the melanotrope cell of Xenopus laevisa. Annals of the New York Academy of Sciences, 839, pp. 229-234. (doi:10.1111/j.1749-6632.1998.tb10765.x)

De Koning, H.P., Watson, C.J., and Jarvis, S.M. (1998) Characterization of a nucleoside/proton symporter in procyclic trypanosoma brucei brucei. Journal of Biological Chemistry, 273(16), pp. 9486-9494. (doi:10.1074/jbc.273.16.9486)

De Koning, H.P., and Jarvis, S.M. (1997) Purine nucleobase transport in bloodstream forms of trypanosoma brucei brucei is mediated by two novel transporters. Molecular and Biochemical Parasitology, 89(2), pp. 245-258. (doi:10.1016/S0166-6851(97)00129-1)

De Koning, H.P., and Jarvis, S.M. (1997) Hypoxanthine iptake through a purine-selective nucleobase transporter in trypano soma brucei brucei procyclic cells is driven by protonmotive force. European Journal of Biochemistry, 247(3), pp. 1102-1110. (doi:10.1111/j.1432-1033.1997.01102.x)

De Koning, H. P., and Jarvis, S. M. (1997) Purine nucleobase transport in bloodstream forms of Trypanosoma brucei brucei. Biochemical Society Transactions, 25, 476S. (doi:10.1042/bst025476s)

Scheenen, W. J.J.M., De Koning, H. P., Jenks, B. G., Vaudry, H., and Roubos, E. W. (1994) The secretion of a-MSH from Xenopus melanotropes involves calcium influx through m-conotoxin-sensitive voltage-operated calcium channels. Journal of Neuroendocrinology, 6(4), pp. 457-464. (doi:10.1111/j.1365-2826.1994.tb00607.x)

De Koning, H.P., Jenks, B.G., and Roubos, E.W. (1993) Analysis of gamma-aminobutyric acidB receptor function in the in vitro and in vivo regulation of alpha-melanotropin-stimulating hormone secretion from melanotrope cells of Xenopus laevis. Endocrinology, 132(2), pp. 674-681. (doi:10.1210/endo.132.2.8381070)

Jenks, B.G., De Koning, H.P., Cruisjen, P.M.J.M., Mauger, C.M.A., Roubos, E.W., Tonon, M.C., Desrues, L., and Vaudry, H. (1993) Analysis of inositol phosphate metabolism in melanotrope cells of Xenopus laevis in relation to background adaptation. Annals of the New York Academy of Sciences, 680, pp. 188-198. (doi:10.1111/j.1749-6632.1993.tb19684.x)

Jenks, B. G., De Koning, H. P., Valentijn, K., and Roubos, E. W. (1993) Dual action of GABAA receptors on the secretory process of melanotrophs of Xenopus laevis. Neuroendocrinology, 58(1), pp. 80-85. (doi:10.1159/000126516)

Leenders, H.J., De Koning, H.P., Ponten, S.P., Jenks, B.G., and Roubos, E.W. (1993) Differential effects of coexisting dopamine, GABA and NPY on α-MSH secretion from melanotrope cells of Xenopus laevis. Life Sciences, 52(24), pp. 1969-1975. (doi:10.1016/0024-3205(93)90638-J)

De Koning, H. P./, Jenks, B. G., Scheenen, W. J.J.M., Balm, P. H.M., and Roubos, E. W. (1992) Analysis of autofeedback mechanisms in the secretion of pro-opiomelanocortin-derived peptides by melanotrope cells of Xenopus laevis. General and Comparative Endocrinology, 87(3), pp. 394-401. (doi:10.1016/0016-6480(92)90046-M)

De Koning, H. P., Jenks, B. G., Huchede, B., and Roubos, E. W. (1992) Dynamics of cyclic-AMP efflux in relation to α-MSH secretion from melanotrope cells of Xenopus laevis. Life Sciences, 51(21), pp. 1667-1673. (doi:10.1016/0024-3205(92)90311-C)

De Koning, H. P., Jenks, B. G., Scheenen, W. J.J.M., de Rijk, E. P.C.T., Cans, R. T.J.M., and Roubos, E. W. (1991) Indirect action of elevated potassium and neuropeptide y on αMSH secretion from the pars intermedia of Xenopus laevi: a biochemical and morphological study. Neuroendocrinology, 54(1), pp. 68-76. (doi:10.1159/000125853)

Jenks, B.G., van Zoest, I.D., De Koning, H.P., Leenders, H.J., and Roubos, E.W. (1991) The CRF-related peptide sauvagine stimulates and the GABAB receptor agonist baclofen inhibits cyclic-AMP production in melanotrope cells of Xenopus laevis. Life Sciences, 48(17), pp. 1633-1637. (doi:10.1016/0024-3205(91)90123-S)

van Arkel, B., van der Baan, J.L., Balt, S., de Bolster, M. W.G., van Delft, R.J., Klumpp, G.W., De Koning, H., and van den Winkel, Y. (1988) A new model for coenzyme B12. Recueil des Travaux Chimiques des Pays-Bas, 107(1), pp. 23-24. (doi:10.1002/recl.19881070106)

Books

De Koning, H. P. (1992) Receptors and Signal Transduction in the Pars Intermedia of Xenopus Laevis. Krips Repro. ISBN 9789090054001

Book Sections

Garcia-Salcedo, J. A., Munday, J. C., Unciti-Broceta, J. D., and De Koning, H. P. (2014) Progress towards new treatments for human African trypanosomiasis. In: Magez, S. and Radwanska, M. (eds.) Trypanosomes and Trypanosomiasis. Springer, pp. 217-238. ISBN 9783709115558 (doi:10.1007/978-3-7091-1556-5_9)

Delespaux, V., and De Koning, H. (2013) Transporters in anti-parasitic drug development and resistance. In: Jäger, T., Koch, O. and Flohé, L. (eds.) Trypanosomatid Diseases: Molecular Routes to Drug Discovery. Series: Drug discovery in infectious diseases (4). John Wiley and Sons Ltd.: Weinheim, pp. 335-349. ISBN 9783527332557 (doi:10.1002/9783527670383.ch18)

Rodenko, B., and De Koning, H. (2013) Rational selection of anti-microbial drug targets: unique or conserved? In: Jäger, T., Koch, O. and Flohé, L. (eds.) Trypanosomatid Diseases: Molecular Routes to Drug Discovery. Series: Drug discovery in infectious diseases (4). Wiley-Blackwell: Weinheim, pp. 279-296. ISBN 9783527332557 (doi:10.1002/9783527670383.ch15)

This list was generated on Sun Jul 24 15:45:24 2016 BST.