Dr David Bhella

  • Programme Leader (Centre for Virus Research)

telephone: 01413303685
email: David.Bhella@glasgow.ac.uk

Research interests

The structural components of viruses represent an intriguing area of study for the structural biologist. In their replication cycle, viruses must generate a protective shell (or capsid) to ferry their genome between hosts. These structural proteins may also be required to facilitate entry to and exit from the host cell, as well as packaging the genome and the necessary functional proteins. In many cases structural proteins are also involved in replication and integration of the viral genome.

The fact that many viruses generate large capsids from only one or two protein species makes them all the more remarkable. Viruses attain this level of economy by assembling their capsids in a highly symmetrical manner making these structures not only scientifically interesting but also beautiful.

My role within the CVR is to apply the techniques of electron-cryomicroscopy and image analysis to the study of viruses. We are equipped with a high-performance JEOL 2200 cryo-microscope suitable both for high-resolution analysis of symmetrical entities e.g. icosahedral and helical viruses, and for tomographic reconstruction of asymmetrical objects such as enveloped viruses and virus-infected cells. We also have facilities for correlative cryo-fluorescence imaging,  high-pressure freezing, automated freeze substitution and cryo-ultramicrotomy. The establishment of these capabilities within a dedicated virus research laboratory gives us an exciting opportunity to visualise the process of virus infection at cellular scale and macromolecular resolution.

We are currently engaged in research into caliciviruses, hepatitis B and C viruses, influenzaviruses and paramyxoviruses (Respiratory Syncytial Virus and Measles Virus).

 

MRC-University of Glasgow Centre for Virus Research

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • A structure analysis of the intact virion and replicative complexes of human respiratory syncytial virus
    Medical Research Council
    2014 - 2017
     

Publications

List by: Type | Date

Jump to: 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2004 | 2003 | 2002 | 2000 | 1999
Number of items: 30.

2014

Hacker, C., Howell, M., Bhella, D., and Lucocq, J. (2014) Strategies for maximizing ATP supply in the microsporidian Encephalitozoon cuniculi: direct binding of mitochondria to the parasitophorous vacuole and clustering of the mitochondrial porin VDAC. Cellular Microbiology, 16 (4). pp. 565-579. ISSN 1462-5814 (doi:10.1111/cmi.12240)

2013

Bakker, S., Duquerroy, S., Galloux, M., Loney, C., Conner, E., Eleouet, J.-F., Rey, F.A., and Bhella, D. (2013) The respiratory syncytial virus nucleoprotein–RNA complex forms a left-handed helical nucleocapsid. Journal of General Virology, 94 (8). pp. 1734-1738. ISSN 0022-1317 (doi:10.1099/vir.0.053025-0)

Rihn, S.J., Wilson, S.J., Loman, N.J., Alim, M., Bakker, S.E., Bhella, D., Gifford, R.J., Rixon, F.J., and Bieniasz, P.D. (2013) Extreme genetic fragility of the HIV-1 capsid. PLoS Pathogens, 9 (6). e1003461. ISSN 1553-7366 (doi:10.1371/journal.ppat.1003461)

Bakker, S. E., Duquerroy, S., Galloux, M., Loney, C., Conner, E., Eleouet, J.-F., Rey, F. A., and Bhella, D. (2013) The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. Journal of General Virology, 94 (Pt_8). pp. 1734-1738. ISSN 0022-1317 (doi:10.1099/vir.0.053025-0)

Vijayakrishnan, S., Loney, C., Jackson, D., Suphamungmee, W., Rixon, F.J., and Bhella, D. (2013) Cryotomography of budding influenza a virus reveals filaments with diverse morphologies that mostly do not bear a genome at their distal end. PLoS Pathogens, 9 (6). e1003413. ISSN 1553-7366 (doi:10.1371/journal.ppat.1003413)

2012

Baines, J.D., Schmid, M.F., Hecksel, C.W., Rochat, R.H., Bhella, D., Chiu, W., and Rixon, F.J. (2012) A tail-like assembly at the portal vertex in intact herpes simplex type-1 virions. PLoS Pathogens, 8 (10). e1002961. ISSN 1553-7366 (doi:10.1371/journal.ppat.1002961)

2011

Bhella, D., and Goodfellow, I.G. (2011) The cryo-electron microscopy structure of feline calicivirus bound to junctional adhesion molecule A at 9-angstrom resolution reveals receptor-induced flexibility and two distinct conformational changes in the capsid protein VP1. Journal of Virology, 85 (21). pp. 11381-11390. ISSN 0022-538X (doi:10.1128/JVI.05621-11)

Neuman, B.W., Kiss, G., Kunding, A.H., Bhella, D., Baksh, M.F., Connelly, S., Droese, B., Klaus, J.P., Makino, S., and Sawicki, S.G. (2011) A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174 (1). pp. 11-22. ISSN 1047-8477 (doi:10.1016/j.jsb.2010.11.021)

2010

Vijayakrishnan, S., Kelly, S.M., Gilbert, R.J.C., Callow, P., Bhella, D., Forsyth, T., Lindsay, J.G., and Byron, O. (2010) Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly. Journal of Molecular Biology, 399 (1). pp. 71-93. ISSN 0022-2836 (doi:10.1016/j.jmb.2010.03.043)

Parsons, J.B., Frank, S., Bhella, D., Liang, M., Prentice, M.B., Mulvihill, D.P., and Warren, M.J. (2010) Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Molecular Cell, 38 (2). pp. 305-315. ISSN 1097-2765 (doi:10.1016/j.molcel.2010.04.008)

Parsons, J.B., Frank, S., Bhella, D., Liang, M., Prentice, M.B., Mulvihill, D.P., and Warren, M. (2010) Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement. Molecular Cell, 38 (2). pp. 305-315. ISSN 1097-2765 (doi:10.1016/j.molcel.2010.04.008)

2009

Tawar, R.G. et al. (2009) Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus. Science, 326 (5957). pp. 1279-1283. ISSN 0036-8075 (doi:10.1126/science.1177634)

Loney, C., Mottet-Osman, G., Roux, L., and Bhella, D. (2009) Paramyxovirus ultrastructure and genome packaging: cryo-electron tomography of Sendai virus. Journal of Virology, 83 (16). pp. 8191-8197. ISSN 0022-538X (doi:10.1128/JVI.00693-09)

Greig, J.A. et al. (2009) Influence of Coagulation Factor X on In Vitro and In Vivo Gene Delivery by Adenovirus (Ad) 5, Ad35, and Chimeric Ad5/Ad35 Vectors. Molecular Therapy, 17 (10). pp. 1683-1691. ISSN 1525-0016 (doi:10.1038/mt.2009.152)

2008

Bhella, D., Gatherer, D., Chaudhry, Y., Pink, R., and Goodfellow, I.G. (2008) Structural insights into calicivirus attachment and uncoating. Journal of Virology, 82 (16). pp. 8051-8058. ISSN 0022-538X (doi:10.1128/JVI.00550-08)

Waddington, S. et al. (2008) Adenovirus serotype 5 hexon mediates liver gene transfer. Cell, 132 (3). pp. 397-409. ISSN 0092-8674 (doi:10.1016/j.cell.2008.01.016 )

2007

Maclellan, K., Loney, C., Yeo, R.P., and Bhella, D. (2007) The 24-angstrom structure of respiratory syncytial virus nucleocapsid protein-RNA decameric rings. Journal of Virology, 81 (17). pp. 9519-9524. ISSN 0022-538X (doi:10.1128/JVI.00526-07)

Tran, T. et al. (2007) The nine C-terminal amino acids of the respiratory syncytial virus protein P are necessary and sufficient for binding to ribonucleoprotein complexes in which six ribonucleotides are contacted per N protein protomer. Journal of general virology, 88 (1). pp. 196-206. ISSN 0022-1317 (doi:10.1099/vir.0.82282-0)

Bhella, D. (2007) Measles virus nucleocapsid structure, conformational flexibility and the rule of six. In: Longhi, S. (ed.) Measles Virus Nucleoprotein. Series: Intrinsically disordered proteins . Nova Publishers, pp. 37-50. ISBN 9781606925218

Cao, Z., Bhella, D., and Lindsay, J.G. (2007) Reconstitution of the mitochondrial PrxII antioxidant defence pathway: General properties and factors affecting PrxII activity and oligomeric state. Journal of Molecular Biology, 372 . pp. 1022-1033. (doi:10.1016/j.jmb.2007.07.018)

Davidson, A.J., and Bhella, D. (2007) Comparative genome and virion structure. In: Arvin, A., Campadelli-Fiume, G. and Mocarski, E. (eds.) Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge University Press. ISBN 9780521827140

2006

Pettigrew, D.M., Williams, D.T., Kerrigan, D., Evans, D.J., Lea, S.M., and Bhella, D. (2006) Structural and functional insights into the interaction of echoviruses and decay-accelerating factor. Journal of Biological Chemistry, 281 . pp. 5169-5177. ISSN 0021-9258 (doi:10.1074/jbc.M510362200)

2004

Bhella, D., Ralph, A., and Yeo, R.P. (2004) Conformational flexibility in recombinant measles virus nucleocapsids visualised by cryo-negative stain electron microscopy and real-space helical reconstruction. Journal of molecular biology, 340 (2). pp. 319-331. ISSN 0022-2836 (doi:10.1016/j.jmb.2004.05.015)

Bhella, D., Goodfellow, I.G., Roversi, P., Pettigrew, D., Chaudhry, Y., Evans, D.J., and Lea, S.M. (2004) The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55). Journal of Biological Chemistry, 279 . pp. 8325-8332. ISSN 0021-9258 (doi:10.1074/jbc.M311334200)

2003

Murphy, L.B., Loney, C., Murray, J., Bhella, D., Ashton, P., and Yeo, R.P. (2003) Investigations into the amino-terminal domain of the respiratory syncytial virus nucleocapsid protein reveal elements important for nucleocapsid formation and interaction with the phosphoprotein. Virology, 307 (1). pp. 143-53. ISSN 0042-6822 (doi:10.1016/S0042-6822(02)00063-6)

2002

Bhella, D., Ralph, A., Murphy, L., and Yeo, R.P. (2002) Significant differences in nucleocapsid morphology within the Paramyxoviridae. Journal of General Virology, 83 (8). pp. 1831-1839. ISSN 0022-1317

Clayton, R.F., Owsianka, A., Aitken, J., Graham, S., Bhella, D., and Patel, A.H. (2002) Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C virus-like particles. Journal of Virology, 76 (18). p. 9562. ISSN 0022-538X (doi:10.1128/jvi.76.18.9562.2002)

McCLelland, D.A., Aitken, J.D., Bhella, D., McNab, D., Mitchell, J., Kelly, S.M., Price, N.C., and Rixon, F.J. (2002) pH reduction as a trigger for dissociation of herpes simplex virus type 1 scaffolds. Journal of Virology, 76 . pp. 7407-7417. ISSN 0022-538X (doi:10.1128/VJI.76.15.7407-7417.2002)

2000

Bhella, D., Rixon, F.J., and Dargan, D.J. (2000) Cryomicroscopy of human cytomegalovirus virions reveals more densely packed genomic DNA than in herpes simplex virus type 1. Journal of Molecular Biology, 295 (2). pp. 155-161. ISSN 0022-2836 (doi:10.1006/jmbi.1999.3344)

1999

AL-Khayat, H.A., Bhella, D., Kenney, J.M., Roth, J.F., Kingsman, A.J., Martin-Rendon, E., and Saibil, H.R. (1999) Yeast Ty retrotransposons assemble into virus-like particles whose T-numbers depend on the C-terminal length of the capsid protein. Journal of molecular biology, 292 (1). pp. 65-73. ISSN 0022-2836 (doi:10.1006/jmbi.1999.3055)

This list was generated on Wed Dec 17 17:05:08 2014 GMT.
Number of items: 30.

Articles

Hacker, C., Howell, M., Bhella, D., and Lucocq, J. (2014) Strategies for maximizing ATP supply in the microsporidian Encephalitozoon cuniculi: direct binding of mitochondria to the parasitophorous vacuole and clustering of the mitochondrial porin VDAC. Cellular Microbiology, 16 (4). pp. 565-579. ISSN 1462-5814 (doi:10.1111/cmi.12240)

Bakker, S., Duquerroy, S., Galloux, M., Loney, C., Conner, E., Eleouet, J.-F., Rey, F.A., and Bhella, D. (2013) The respiratory syncytial virus nucleoprotein–RNA complex forms a left-handed helical nucleocapsid. Journal of General Virology, 94 (8). pp. 1734-1738. ISSN 0022-1317 (doi:10.1099/vir.0.053025-0)

Rihn, S.J., Wilson, S.J., Loman, N.J., Alim, M., Bakker, S.E., Bhella, D., Gifford, R.J., Rixon, F.J., and Bieniasz, P.D. (2013) Extreme genetic fragility of the HIV-1 capsid. PLoS Pathogens, 9 (6). e1003461. ISSN 1553-7366 (doi:10.1371/journal.ppat.1003461)

Bakker, S. E., Duquerroy, S., Galloux, M., Loney, C., Conner, E., Eleouet, J.-F., Rey, F. A., and Bhella, D. (2013) The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. Journal of General Virology, 94 (Pt_8). pp. 1734-1738. ISSN 0022-1317 (doi:10.1099/vir.0.053025-0)

Vijayakrishnan, S., Loney, C., Jackson, D., Suphamungmee, W., Rixon, F.J., and Bhella, D. (2013) Cryotomography of budding influenza a virus reveals filaments with diverse morphologies that mostly do not bear a genome at their distal end. PLoS Pathogens, 9 (6). e1003413. ISSN 1553-7366 (doi:10.1371/journal.ppat.1003413)

Baines, J.D., Schmid, M.F., Hecksel, C.W., Rochat, R.H., Bhella, D., Chiu, W., and Rixon, F.J. (2012) A tail-like assembly at the portal vertex in intact herpes simplex type-1 virions. PLoS Pathogens, 8 (10). e1002961. ISSN 1553-7366 (doi:10.1371/journal.ppat.1002961)

Bhella, D., and Goodfellow, I.G. (2011) The cryo-electron microscopy structure of feline calicivirus bound to junctional adhesion molecule A at 9-angstrom resolution reveals receptor-induced flexibility and two distinct conformational changes in the capsid protein VP1. Journal of Virology, 85 (21). pp. 11381-11390. ISSN 0022-538X (doi:10.1128/JVI.05621-11)

Neuman, B.W., Kiss, G., Kunding, A.H., Bhella, D., Baksh, M.F., Connelly, S., Droese, B., Klaus, J.P., Makino, S., and Sawicki, S.G. (2011) A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174 (1). pp. 11-22. ISSN 1047-8477 (doi:10.1016/j.jsb.2010.11.021)

Vijayakrishnan, S., Kelly, S.M., Gilbert, R.J.C., Callow, P., Bhella, D., Forsyth, T., Lindsay, J.G., and Byron, O. (2010) Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly. Journal of Molecular Biology, 399 (1). pp. 71-93. ISSN 0022-2836 (doi:10.1016/j.jmb.2010.03.043)

Parsons, J.B., Frank, S., Bhella, D., Liang, M., Prentice, M.B., Mulvihill, D.P., and Warren, M.J. (2010) Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Molecular Cell, 38 (2). pp. 305-315. ISSN 1097-2765 (doi:10.1016/j.molcel.2010.04.008)

Parsons, J.B., Frank, S., Bhella, D., Liang, M., Prentice, M.B., Mulvihill, D.P., and Warren, M. (2010) Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement. Molecular Cell, 38 (2). pp. 305-315. ISSN 1097-2765 (doi:10.1016/j.molcel.2010.04.008)

Tawar, R.G. et al. (2009) Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus. Science, 326 (5957). pp. 1279-1283. ISSN 0036-8075 (doi:10.1126/science.1177634)

Loney, C., Mottet-Osman, G., Roux, L., and Bhella, D. (2009) Paramyxovirus ultrastructure and genome packaging: cryo-electron tomography of Sendai virus. Journal of Virology, 83 (16). pp. 8191-8197. ISSN 0022-538X (doi:10.1128/JVI.00693-09)

Greig, J.A. et al. (2009) Influence of Coagulation Factor X on In Vitro and In Vivo Gene Delivery by Adenovirus (Ad) 5, Ad35, and Chimeric Ad5/Ad35 Vectors. Molecular Therapy, 17 (10). pp. 1683-1691. ISSN 1525-0016 (doi:10.1038/mt.2009.152)

Bhella, D., Gatherer, D., Chaudhry, Y., Pink, R., and Goodfellow, I.G. (2008) Structural insights into calicivirus attachment and uncoating. Journal of Virology, 82 (16). pp. 8051-8058. ISSN 0022-538X (doi:10.1128/JVI.00550-08)

Waddington, S. et al. (2008) Adenovirus serotype 5 hexon mediates liver gene transfer. Cell, 132 (3). pp. 397-409. ISSN 0092-8674 (doi:10.1016/j.cell.2008.01.016 )

Maclellan, K., Loney, C., Yeo, R.P., and Bhella, D. (2007) The 24-angstrom structure of respiratory syncytial virus nucleocapsid protein-RNA decameric rings. Journal of Virology, 81 (17). pp. 9519-9524. ISSN 0022-538X (doi:10.1128/JVI.00526-07)

Tran, T. et al. (2007) The nine C-terminal amino acids of the respiratory syncytial virus protein P are necessary and sufficient for binding to ribonucleoprotein complexes in which six ribonucleotides are contacted per N protein protomer. Journal of general virology, 88 (1). pp. 196-206. ISSN 0022-1317 (doi:10.1099/vir.0.82282-0)

Cao, Z., Bhella, D., and Lindsay, J.G. (2007) Reconstitution of the mitochondrial PrxII antioxidant defence pathway: General properties and factors affecting PrxII activity and oligomeric state. Journal of Molecular Biology, 372 . pp. 1022-1033. (doi:10.1016/j.jmb.2007.07.018)

Pettigrew, D.M., Williams, D.T., Kerrigan, D., Evans, D.J., Lea, S.M., and Bhella, D. (2006) Structural and functional insights into the interaction of echoviruses and decay-accelerating factor. Journal of Biological Chemistry, 281 . pp. 5169-5177. ISSN 0021-9258 (doi:10.1074/jbc.M510362200)

Bhella, D., Ralph, A., and Yeo, R.P. (2004) Conformational flexibility in recombinant measles virus nucleocapsids visualised by cryo-negative stain electron microscopy and real-space helical reconstruction. Journal of molecular biology, 340 (2). pp. 319-331. ISSN 0022-2836 (doi:10.1016/j.jmb.2004.05.015)

Bhella, D., Goodfellow, I.G., Roversi, P., Pettigrew, D., Chaudhry, Y., Evans, D.J., and Lea, S.M. (2004) The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55). Journal of Biological Chemistry, 279 . pp. 8325-8332. ISSN 0021-9258 (doi:10.1074/jbc.M311334200)

Murphy, L.B., Loney, C., Murray, J., Bhella, D., Ashton, P., and Yeo, R.P. (2003) Investigations into the amino-terminal domain of the respiratory syncytial virus nucleocapsid protein reveal elements important for nucleocapsid formation and interaction with the phosphoprotein. Virology, 307 (1). pp. 143-53. ISSN 0042-6822 (doi:10.1016/S0042-6822(02)00063-6)

Bhella, D., Ralph, A., Murphy, L., and Yeo, R.P. (2002) Significant differences in nucleocapsid morphology within the Paramyxoviridae. Journal of General Virology, 83 (8). pp. 1831-1839. ISSN 0022-1317

Clayton, R.F., Owsianka, A., Aitken, J., Graham, S., Bhella, D., and Patel, A.H. (2002) Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C virus-like particles. Journal of Virology, 76 (18). p. 9562. ISSN 0022-538X (doi:10.1128/jvi.76.18.9562.2002)

McCLelland, D.A., Aitken, J.D., Bhella, D., McNab, D., Mitchell, J., Kelly, S.M., Price, N.C., and Rixon, F.J. (2002) pH reduction as a trigger for dissociation of herpes simplex virus type 1 scaffolds. Journal of Virology, 76 . pp. 7407-7417. ISSN 0022-538X (doi:10.1128/VJI.76.15.7407-7417.2002)

Bhella, D., Rixon, F.J., and Dargan, D.J. (2000) Cryomicroscopy of human cytomegalovirus virions reveals more densely packed genomic DNA than in herpes simplex virus type 1. Journal of Molecular Biology, 295 (2). pp. 155-161. ISSN 0022-2836 (doi:10.1006/jmbi.1999.3344)

AL-Khayat, H.A., Bhella, D., Kenney, J.M., Roth, J.F., Kingsman, A.J., Martin-Rendon, E., and Saibil, H.R. (1999) Yeast Ty retrotransposons assemble into virus-like particles whose T-numbers depend on the C-terminal length of the capsid protein. Journal of molecular biology, 292 (1). pp. 65-73. ISSN 0022-2836 (doi:10.1006/jmbi.1999.3055)

Book Sections

Bhella, D. (2007) Measles virus nucleocapsid structure, conformational flexibility and the rule of six. In: Longhi, S. (ed.) Measles Virus Nucleoprotein. Series: Intrinsically disordered proteins . Nova Publishers, pp. 37-50. ISBN 9781606925218

Davidson, A.J., and Bhella, D. (2007) Comparative genome and virion structure. In: Arvin, A., Campadelli-Fiume, G. and Mocarski, E. (eds.) Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge University Press. ISBN 9780521827140

This list was generated on Wed Dec 17 17:05:08 2014 GMT.