Dr David Bhella

  • Programme Leader (Centre for Virus Research)

telephone: 01413303685
email: David.Bhella@glasgow.ac.uk

Research interests

CVR logo

The structural components of viruses represent an intriguing area of study for the structural biologist. In their replication cycle, viruses must generate a protective shell (or capsid) to ferry their genome between hosts. These structural proteins may also be required to facilitate entry to and exit from the host cell, as well as packaging the genome and the necessary functional proteins. In many cases structural proteins are also involved in replication and integration of the viral genome.

The fact that many viruses generate large capsids from only one or two protein species makes them all the more remarkable. Viruses attain this level of economy by assembling their capsids in a highly symmetrical manner making these structures not only scientifically interesting but also beautiful.

My role within the CVR is to apply the techniques of electron-cryomicroscopy and image analysis to the study of viruses. We are equipped with a high-performance JEOL 2200 cryo-microscope suitable both for high-resolution analysis of symmetrical entities e.g. icosahedral and helical viruses, and for tomographic reconstruction of asymmetrical objects such as enveloped viruses and virus-infected cells. We also have facilities for correlative cryo-fluorescence imaging,  high-pressure freezing, automated freeze substitution and cryo-ultramicrotomy. The establishment of these capabilities within a dedicated virus research laboratory gives us an exciting opportunity to visualise the process of virus infection at cellular scale and macromolecular resolution.

We are currently engaged in research into caliciviruses, hepatitis B and C viruses, influenzaviruses and paramyxoviruses (Respiratory Syncytial Virus and Measles Virus).

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • A structure analysis of the intact virion and replicative complexes of human respiratory syncytial virus
    Medical Research Council
    2014 - 2017
     

Publications

List by: Type | Date

Jump to: 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2004 | 2003 | 2002 | 2000 | 1999
Number of items: 33.

2015

Bhella, D. (2015) The role of cellular adhesion molecules in virus attachment and entry. Philosophical Transactions of the Royal Society B: Biological Sciences, 370(1663). (doi:10.1098/rstb.2014.0035)

Fan, W. H., Robertson, A. P.E., McElwee, M., Bhella, D., Rixon, F. J., and Lauder, R. (2015) The large tegument protein pUL36 is essential for formation of the capsid vertex-specific component at the capsid-tegument interface of herpes simplex virus 1. Journal of Virology, 89(3). pp. 1502-1511. (doi:10.1128/JVI.02887-14)

McGonigle, R., Yap, W. B., Ong, S. T., Gatherer, D., Bakker, S. E., Tan, W. S., and Bhella, D. (2015) An N-terminal extension to the hepatitis B virus core protein forms a poorly ordered trimeric spike in assembled virus-like particles. Journal of Structural Biology, 189(2). pp. 73-80. (doi:10.1016/j.jsb.2014.12.006)

2014

Hacker, C., Howell, M., Bhella, D., and Lucocq, J. (2014) Strategies for maximizing ATP supply in the microsporidian Encephalitozoon cuniculi: direct binding of mitochondria to the parasitophorous vacuole and clustering of the mitochondrial porin VDAC. Cellular Microbiology, 16(4). pp. 565-579. (doi:10.1111/cmi.12240)

2013

Bakker, S., Duquerroy, S., Galloux, M., Loney, C., Conner, E., Eleouet, J.-F., Rey, F.A., and Bhella, D. (2013) The respiratory syncytial virus nucleoprotein–RNA complex forms a left-handed helical nucleocapsid. Journal of General Virology, 94(8). pp. 1734-1738. (doi:10.1099/vir.0.053025-0)

Rihn, S.J., Wilson, S.J., Loman, N.J., Alim, M., Bakker, S.E., Bhella, D., Gifford, R.J., Rixon, F.J., and Bieniasz, P.D. (2013) Extreme genetic fragility of the HIV-1 capsid. PLoS Pathogens, 9(6). e1003461. (doi:10.1371/journal.ppat.1003461)

Bakker, S. E., Duquerroy, S., Galloux, M., Loney, C., Conner, E., Eleouet, J.-F., Rey, F. A., and Bhella, D. (2013) The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. Journal of General Virology, 94(Pt_8). pp. 1734-1738. (doi:10.1099/vir.0.053025-0)

Vijayakrishnan, S., Loney, C., Jackson, D., Suphamungmee, W., Rixon, F.J., and Bhella, D. (2013) Cryotomography of budding influenza a virus reveals filaments with diverse morphologies that mostly do not bear a genome at their distal end. PLoS Pathogens, 9(6). e1003413. (doi:10.1371/journal.ppat.1003413)

2012

Baines, J.D., Schmid, M.F., Hecksel, C.W., Rochat, R.H., Bhella, D., Chiu, W., and Rixon, F.J. (2012) A tail-like assembly at the portal vertex in intact herpes simplex type-1 virions. PLoS Pathogens, 8(10). e1002961. (doi:10.1371/journal.ppat.1002961)

2011

Bhella, D., and Goodfellow, I.G. (2011) The cryo-electron microscopy structure of feline calicivirus bound to junctional adhesion molecule A at 9-angstrom resolution reveals receptor-induced flexibility and two distinct conformational changes in the capsid protein VP1. Journal of Virology, 85(21). pp. 11381-11390. (doi:10.1128/JVI.05621-11)

Neuman, B.W., Kiss, G., Kunding, A.H., Bhella, D., Baksh, M.F., Connelly, S., Droese, B., Klaus, J.P., Makino, S., and Sawicki, S.G. (2011) A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174(1). pp. 11-22. (doi:10.1016/j.jsb.2010.11.021)

2010

Vijayakrishnan, S., Kelly, S.M., Gilbert, R.J.C., Callow, P., Bhella, D., Forsyth, T., Lindsay, J.G., and Byron, O. (2010) Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly. Journal of Molecular Biology, 399(1). pp. 71-93. (doi:10.1016/j.jmb.2010.03.043)

Parsons, J.B., Frank, S., Bhella, D., Liang, M., Prentice, M.B., Mulvihill, D.P., and Warren, M.J. (2010) Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Molecular Cell, 38(2). pp. 305-315. (doi:10.1016/j.molcel.2010.04.008)

Parsons, J.B., Frank, S., Bhella, D., Liang, M., Prentice, M.B., Mulvihill, D.P., and Warren, M. (2010) Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement. Molecular Cell, 38(2). pp. 305-315. (doi:10.1016/j.molcel.2010.04.008)

2009

Tawar, R.G. et al. (2009) Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus. Science, 326(5957). pp. 1279-1283. (doi:10.1126/science.1177634)

Loney, C., Mottet-Osman, G., Roux, L., and Bhella, D. (2009) Paramyxovirus ultrastructure and genome packaging: cryo-electron tomography of Sendai virus. Journal of Virology, 83(16). pp. 8191-8197. (doi:10.1128/JVI.00693-09)

Greig, J.A. et al. (2009) Influence of Coagulation Factor X on In Vitro and In Vivo Gene Delivery by Adenovirus (Ad) 5, Ad35, and Chimeric Ad5/Ad35 Vectors. Molecular Therapy, 17(10). pp. 1683-1691. (doi:10.1038/mt.2009.152)

2008

Bhella, D., Gatherer, D., Chaudhry, Y., Pink, R., and Goodfellow, I.G. (2008) Structural insights into calicivirus attachment and uncoating. Journal of Virology, 82(16). pp. 8051-8058. (doi:10.1128/JVI.00550-08)

Waddington, S. et al. (2008) Adenovirus serotype 5 hexon mediates liver gene transfer. Cell, 132(3). pp. 397-409. (doi:10.1016/j.cell.2008.01.016 )

2007

Maclellan, K., Loney, C., Yeo, R.P., and Bhella, D. (2007) The 24-angstrom structure of respiratory syncytial virus nucleocapsid protein-RNA decameric rings. Journal of Virology, 81(17). pp. 9519-9524. (doi:10.1128/JVI.00526-07)

Tran, T. et al. (2007) The nine C-terminal amino acids of the respiratory syncytial virus protein P are necessary and sufficient for binding to ribonucleoprotein complexes in which six ribonucleotides are contacted per N protein protomer. Journal of general virology, 88(1). pp. 196-206. (doi:10.1099/vir.0.82282-0)

Bhella, D. (2007) Measles virus nucleocapsid structure, conformational flexibility and the rule of six. In: Longhi, S. (ed.) Measles Virus Nucleoprotein. Series: Intrinsically disordered proteins. Nova Publishers, pp. 37-50. ISBN 9781606925218

Cao, Z., Bhella, D., and Lindsay, J.G. (2007) Reconstitution of the mitochondrial PrxII antioxidant defence pathway: General properties and factors affecting PrxII activity and oligomeric state. Journal of Molecular Biology, 372. pp. 1022-1033. (doi:10.1016/j.jmb.2007.07.018)

Davidson, A.J., and Bhella, D. (2007) Comparative genome and virion structure. In: Arvin, A., Campadelli-Fiume, G. and Mocarski, E. (eds.) Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge University Press. ISBN 9780521827140

2006

Pettigrew, D.M., Williams, D.T., Kerrigan, D., Evans, D.J., Lea, S.M., and Bhella, D. (2006) Structural and functional insights into the interaction of echoviruses and decay-accelerating factor. Journal of Biological Chemistry, 281. pp. 5169-5177. (doi:10.1074/jbc.M510362200)

2004

Bhella, D., Ralph, A., and Yeo, R.P. (2004) Conformational flexibility in recombinant measles virus nucleocapsids visualised by cryo-negative stain electron microscopy and real-space helical reconstruction. Journal of molecular biology, 340(2). pp. 319-331. (doi:10.1016/j.jmb.2004.05.015)

Bhella, D., Goodfellow, I.G., Roversi, P., Pettigrew, D., Chaudhry, Y., Evans, D.J., and Lea, S.M. (2004) The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55). Journal of Biological Chemistry, 279. pp. 8325-8332. (doi:10.1074/jbc.M311334200)

2003

Murphy, L.B., Loney, C., Murray, J., Bhella, D., Ashton, P., and Yeo, R.P. (2003) Investigations into the amino-terminal domain of the respiratory syncytial virus nucleocapsid protein reveal elements important for nucleocapsid formation and interaction with the phosphoprotein. Virology, 307(1). pp. 143-53. (doi:10.1016/S0042-6822(02)00063-6)

2002

Bhella, D., Ralph, A., Murphy, L., and Yeo, R.P. (2002) Significant differences in nucleocapsid morphology within the Paramyxoviridae. Journal of General Virology, 83(8). pp. 1831-1839.

Clayton, R.F., Owsianka, A., Aitken, J., Graham, S., Bhella, D., and Patel, A.H. (2002) Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C virus-like particles. Journal of Virology, 76(18). p. 9562. (doi:10.1128/jvi.76.18.9562.2002)

McCLelland, D.A., Aitken, J.D., Bhella, D., McNab, D., Mitchell, J., Kelly, S.M., Price, N.C., and Rixon, F.J. (2002) pH reduction as a trigger for dissociation of herpes simplex virus type 1 scaffolds. Journal of Virology, 76. pp. 7407-7417. (doi:10.1128/VJI.76.15.7407-7417.2002)

2000

Bhella, D., Rixon, F.J., and Dargan, D.J. (2000) Cryomicroscopy of human cytomegalovirus virions reveals more densely packed genomic DNA than in herpes simplex virus type 1. Journal of Molecular Biology, 295(2). pp. 155-161. (doi:10.1006/jmbi.1999.3344)

1999

AL-Khayat, H.A., Bhella, D., Kenney, J.M., Roth, J.F., Kingsman, A.J., Martin-Rendon, E., and Saibil, H.R. (1999) Yeast Ty retrotransposons assemble into virus-like particles whose T-numbers depend on the C-terminal length of the capsid protein. Journal of molecular biology, 292(1). pp. 65-73. (doi:10.1006/jmbi.1999.3055)

This list was generated on Fri Feb 27 13:07:37 2015 GMT.
Number of items: 33.

Articles

Bhella, D. (2015) The role of cellular adhesion molecules in virus attachment and entry. Philosophical Transactions of the Royal Society B: Biological Sciences, 370(1663). (doi:10.1098/rstb.2014.0035)

Fan, W. H., Robertson, A. P.E., McElwee, M., Bhella, D., Rixon, F. J., and Lauder, R. (2015) The large tegument protein pUL36 is essential for formation of the capsid vertex-specific component at the capsid-tegument interface of herpes simplex virus 1. Journal of Virology, 89(3). pp. 1502-1511. (doi:10.1128/JVI.02887-14)

McGonigle, R., Yap, W. B., Ong, S. T., Gatherer, D., Bakker, S. E., Tan, W. S., and Bhella, D. (2015) An N-terminal extension to the hepatitis B virus core protein forms a poorly ordered trimeric spike in assembled virus-like particles. Journal of Structural Biology, 189(2). pp. 73-80. (doi:10.1016/j.jsb.2014.12.006)

Hacker, C., Howell, M., Bhella, D., and Lucocq, J. (2014) Strategies for maximizing ATP supply in the microsporidian Encephalitozoon cuniculi: direct binding of mitochondria to the parasitophorous vacuole and clustering of the mitochondrial porin VDAC. Cellular Microbiology, 16(4). pp. 565-579. (doi:10.1111/cmi.12240)

Bakker, S., Duquerroy, S., Galloux, M., Loney, C., Conner, E., Eleouet, J.-F., Rey, F.A., and Bhella, D. (2013) The respiratory syncytial virus nucleoprotein–RNA complex forms a left-handed helical nucleocapsid. Journal of General Virology, 94(8). pp. 1734-1738. (doi:10.1099/vir.0.053025-0)

Rihn, S.J., Wilson, S.J., Loman, N.J., Alim, M., Bakker, S.E., Bhella, D., Gifford, R.J., Rixon, F.J., and Bieniasz, P.D. (2013) Extreme genetic fragility of the HIV-1 capsid. PLoS Pathogens, 9(6). e1003461. (doi:10.1371/journal.ppat.1003461)

Bakker, S. E., Duquerroy, S., Galloux, M., Loney, C., Conner, E., Eleouet, J.-F., Rey, F. A., and Bhella, D. (2013) The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. Journal of General Virology, 94(Pt_8). pp. 1734-1738. (doi:10.1099/vir.0.053025-0)

Vijayakrishnan, S., Loney, C., Jackson, D., Suphamungmee, W., Rixon, F.J., and Bhella, D. (2013) Cryotomography of budding influenza a virus reveals filaments with diverse morphologies that mostly do not bear a genome at their distal end. PLoS Pathogens, 9(6). e1003413. (doi:10.1371/journal.ppat.1003413)

Baines, J.D., Schmid, M.F., Hecksel, C.W., Rochat, R.H., Bhella, D., Chiu, W., and Rixon, F.J. (2012) A tail-like assembly at the portal vertex in intact herpes simplex type-1 virions. PLoS Pathogens, 8(10). e1002961. (doi:10.1371/journal.ppat.1002961)

Bhella, D., and Goodfellow, I.G. (2011) The cryo-electron microscopy structure of feline calicivirus bound to junctional adhesion molecule A at 9-angstrom resolution reveals receptor-induced flexibility and two distinct conformational changes in the capsid protein VP1. Journal of Virology, 85(21). pp. 11381-11390. (doi:10.1128/JVI.05621-11)

Neuman, B.W., Kiss, G., Kunding, A.H., Bhella, D., Baksh, M.F., Connelly, S., Droese, B., Klaus, J.P., Makino, S., and Sawicki, S.G. (2011) A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174(1). pp. 11-22. (doi:10.1016/j.jsb.2010.11.021)

Vijayakrishnan, S., Kelly, S.M., Gilbert, R.J.C., Callow, P., Bhella, D., Forsyth, T., Lindsay, J.G., and Byron, O. (2010) Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly. Journal of Molecular Biology, 399(1). pp. 71-93. (doi:10.1016/j.jmb.2010.03.043)

Parsons, J.B., Frank, S., Bhella, D., Liang, M., Prentice, M.B., Mulvihill, D.P., and Warren, M.J. (2010) Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Molecular Cell, 38(2). pp. 305-315. (doi:10.1016/j.molcel.2010.04.008)

Parsons, J.B., Frank, S., Bhella, D., Liang, M., Prentice, M.B., Mulvihill, D.P., and Warren, M. (2010) Synthesis of Empty Bacterial Microcompartments, Directed Organelle Protein Incorporation, and Evidence of Filament-Associated Organelle Movement. Molecular Cell, 38(2). pp. 305-315. (doi:10.1016/j.molcel.2010.04.008)

Tawar, R.G. et al. (2009) Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus. Science, 326(5957). pp. 1279-1283. (doi:10.1126/science.1177634)

Loney, C., Mottet-Osman, G., Roux, L., and Bhella, D. (2009) Paramyxovirus ultrastructure and genome packaging: cryo-electron tomography of Sendai virus. Journal of Virology, 83(16). pp. 8191-8197. (doi:10.1128/JVI.00693-09)

Greig, J.A. et al. (2009) Influence of Coagulation Factor X on In Vitro and In Vivo Gene Delivery by Adenovirus (Ad) 5, Ad35, and Chimeric Ad5/Ad35 Vectors. Molecular Therapy, 17(10). pp. 1683-1691. (doi:10.1038/mt.2009.152)

Bhella, D., Gatherer, D., Chaudhry, Y., Pink, R., and Goodfellow, I.G. (2008) Structural insights into calicivirus attachment and uncoating. Journal of Virology, 82(16). pp. 8051-8058. (doi:10.1128/JVI.00550-08)

Waddington, S. et al. (2008) Adenovirus serotype 5 hexon mediates liver gene transfer. Cell, 132(3). pp. 397-409. (doi:10.1016/j.cell.2008.01.016 )

Maclellan, K., Loney, C., Yeo, R.P., and Bhella, D. (2007) The 24-angstrom structure of respiratory syncytial virus nucleocapsid protein-RNA decameric rings. Journal of Virology, 81(17). pp. 9519-9524. (doi:10.1128/JVI.00526-07)

Tran, T. et al. (2007) The nine C-terminal amino acids of the respiratory syncytial virus protein P are necessary and sufficient for binding to ribonucleoprotein complexes in which six ribonucleotides are contacted per N protein protomer. Journal of general virology, 88(1). pp. 196-206. (doi:10.1099/vir.0.82282-0)

Cao, Z., Bhella, D., and Lindsay, J.G. (2007) Reconstitution of the mitochondrial PrxII antioxidant defence pathway: General properties and factors affecting PrxII activity and oligomeric state. Journal of Molecular Biology, 372. pp. 1022-1033. (doi:10.1016/j.jmb.2007.07.018)

Pettigrew, D.M., Williams, D.T., Kerrigan, D., Evans, D.J., Lea, S.M., and Bhella, D. (2006) Structural and functional insights into the interaction of echoviruses and decay-accelerating factor. Journal of Biological Chemistry, 281. pp. 5169-5177. (doi:10.1074/jbc.M510362200)

Bhella, D., Ralph, A., and Yeo, R.P. (2004) Conformational flexibility in recombinant measles virus nucleocapsids visualised by cryo-negative stain electron microscopy and real-space helical reconstruction. Journal of molecular biology, 340(2). pp. 319-331. (doi:10.1016/j.jmb.2004.05.015)

Bhella, D., Goodfellow, I.G., Roversi, P., Pettigrew, D., Chaudhry, Y., Evans, D.J., and Lea, S.M. (2004) The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55). Journal of Biological Chemistry, 279. pp. 8325-8332. (doi:10.1074/jbc.M311334200)

Murphy, L.B., Loney, C., Murray, J., Bhella, D., Ashton, P., and Yeo, R.P. (2003) Investigations into the amino-terminal domain of the respiratory syncytial virus nucleocapsid protein reveal elements important for nucleocapsid formation and interaction with the phosphoprotein. Virology, 307(1). pp. 143-53. (doi:10.1016/S0042-6822(02)00063-6)

Bhella, D., Ralph, A., Murphy, L., and Yeo, R.P. (2002) Significant differences in nucleocapsid morphology within the Paramyxoviridae. Journal of General Virology, 83(8). pp. 1831-1839.

Clayton, R.F., Owsianka, A., Aitken, J., Graham, S., Bhella, D., and Patel, A.H. (2002) Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C virus-like particles. Journal of Virology, 76(18). p. 9562. (doi:10.1128/jvi.76.18.9562.2002)

McCLelland, D.A., Aitken, J.D., Bhella, D., McNab, D., Mitchell, J., Kelly, S.M., Price, N.C., and Rixon, F.J. (2002) pH reduction as a trigger for dissociation of herpes simplex virus type 1 scaffolds. Journal of Virology, 76. pp. 7407-7417. (doi:10.1128/VJI.76.15.7407-7417.2002)

Bhella, D., Rixon, F.J., and Dargan, D.J. (2000) Cryomicroscopy of human cytomegalovirus virions reveals more densely packed genomic DNA than in herpes simplex virus type 1. Journal of Molecular Biology, 295(2). pp. 155-161. (doi:10.1006/jmbi.1999.3344)

AL-Khayat, H.A., Bhella, D., Kenney, J.M., Roth, J.F., Kingsman, A.J., Martin-Rendon, E., and Saibil, H.R. (1999) Yeast Ty retrotransposons assemble into virus-like particles whose T-numbers depend on the C-terminal length of the capsid protein. Journal of molecular biology, 292(1). pp. 65-73. (doi:10.1006/jmbi.1999.3055)

Book Sections

Bhella, D. (2007) Measles virus nucleocapsid structure, conformational flexibility and the rule of six. In: Longhi, S. (ed.) Measles Virus Nucleoprotein. Series: Intrinsically disordered proteins. Nova Publishers, pp. 37-50. ISBN 9781606925218

Davidson, A.J., and Bhella, D. (2007) Comparative genome and virion structure. In: Arvin, A., Campadelli-Fiume, G. and Mocarski, E. (eds.) Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge University Press. ISBN 9780521827140

This list was generated on Fri Feb 27 13:07:37 2015 GMT.