Professor Graeme Milligan

  • Gardiner Chair of Biochemistry (Institute of Molecular Cell and Systems Biology)
  • Dean of Research (MVLS College Senior Management)

telephone: 01413305557
email: graeme.milligan@glasgow.ac.uk

R253 Level 2
MVLS College Office
West Medical Building
Glasgow G12 8QQ


'G-protein coupled receptors, G-proteins and signal transduction'

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Number of items: 233.

2014

Hudson, B. D., Christiansen, E., Murdoch, H., Jenkins, L., Hansen, A. H., Madsen, O., Ulven, T., and Milligan, G. (2014) Complex pharmacology of novel allosteric free fatty acid 3 receptor ligands. Molecular Pharmacology, 86 (2). pp. 200-210. ISSN 0026-895X (doi:10.1124/mol.114.093294)

Hudson, B. D., Shimpukade, B., Milligan, G., and Ulven, T. (2014) The molecular basis of ligand interaction at free fatty acid receptor 4 (FFA4/GPR120). Journal of Biological Chemistry, 289 . pp. 20345-20358. ISSN 0021-9258 (doi:10.1074/jbc.M114.561449)

Zakrys, L., Ward, R. J., Pediani, J. D., Godin, A. G., Graham, G. J., and Milligan, G. (2014) Roundabout 1 exists predominantly as a basal dimeric complex and this is unaffected by binding of the ligand Slit2. Biochemical Journal, 461 (1). pp. 61-73. ISSN 0264-6021 (doi:10.1042/BJ20140190)

Butcher, A. J., Hudson, B. D., Shimpukade, B., Alvarez-Curto, E., Prihandoko, R., Ulven, T., Milligan, G., and Tobin, A. B. (2014) Concomitant action of structural elements and receptor phosphorylation determine arrestin-3 interaction with the free fatty acid receptor FFA4. Journal of Biological Chemistry, 289 . pp. 18451-18465. ISSN 0021-9258 (doi:10.1074/jbc.M114.568816)

Fjaere, E. et al. (2014) Indomethacin treatment prevents high fat diet-induced obesity and insulin resistance but not glucose intolerance in C57BL/6J mice. Journal of Biological Chemistry, 289 (23). pp. 16032-16045. ISSN 0021-9258 (doi:10.1074/jbc.M113.525220)

McKinney, C. A., Fattah, C., Loughrey, C. M., Milligan, G., and Nicklin, S. A. (2014) Angiotensin-(1-7) and angiotensin-(1-9): function in cardiac and vascular remodeling. Clinical Science, 126 . pp. 815-827. ISSN 0143-5221 (doi:10.1042/CS20130436)

Milligan, G., Ulven, T., Murdoch, H., and Hudson, B. D. (2014) G-protein-coupled receptors for free fatty acids: nutritional and therapeutic targets. British Journal of Nutrition, 111 (S1). S3-S7. ISSN 0007-1145 (doi:10.1017/S0007114513002249)

Ferre, S., Casado, V., Devi, L. A., Filizola, M., Jockers, R., Lohse, M. J., Milligan, G., Pin, J.-P., and Guitart, X. (2014) G protein-coupled receptor oligomerization revisited: functional and pharmacological perspectives. Pharmacological Reviews, 66 (2). pp. 413-434. ISSN 0031-6997 (doi:10.1124/pr.113.008052)

Ward, R.J., and Milligan, G. (2014) Structural and biophysical characterisation of G protein-coupled receptor ligand binding using resonance energy transfer and fluorescent labelling techniques. Biochimica et Biophysica Acta (BBA): Biomembranes, 1838 (1A). pp. 3-14. ISSN 0005-2736 (doi:10.1016/j.bbamem.2013.04.007)

Mackenzie, A.E. et al. (2014) The antiallergic mast cell stabilizers lodoxamide and bufrolin as the first high and equipotent agonists of human and rat gpr35. Molecular Pharmacology, 85 (1). pp. 91-104. ISSN 0026-895X (doi:10.1124/mol.113.089482)

Watterson, K. R., Hudson, B. D., Ulven, T., and Milligan, G. (2014) Treatment of type 2 diabetes by free fatty acid receptor agonists. Frontiers in Endocrinology . ISSN 1664-2392 (doi:10.3389/fendo.2014.00137) (Early Online Publication)

2013

Horobin, R.W., Rashid-Doubell, F., Pediani, J.D., and Milligan, G. (2013) Predicting small molecule fluorescent probe localization in living cells using QSAR modeling. 1. Overview and models for probes of structure, properties and function in single cells. Biotechnic and Histochemistry, 88 (8). pp. 440-460. ISSN 1052-0295 (doi:10.3109/10520295.2013.780634)

Hudson, B.D., Shimpukade, B., Mackenzie, A.E., Butcher, A.J., Pediani, J.D., Christiansen, E., Heathcote, H., Tobin, A.B., Ulven, T., and Milligan, G. (2013) The pharmacology of TUG-891, a potent and selective agonist of the free fatty acid receptor 4 (FFA4/GPR120), demonstrates both potential opportunity and possible challenges to therapeutic agonism. Molecular Pharmacology, 84 (5). pp. 710-725. ISSN 0026-895X (doi:10.1124/mol.113.087783)

Perez-Aso, M., Segura, V., Montó, F., Barettino, D., Noguera, M.A., Milligan, G., and D'Ocon, P. (2013) The three α1-adrenoceptor subtypes show different spatio-temporal mechanisms of internalization and ERK1/2 phosphorylation. Biochimica et Biophysica Acta: Molecular Cell Research, 1833 (10). pp. 2322-2333. ISSN 0167-4889 (doi:10.1016/j.bbamcr.2013.06.013)

Scott, E., Loya, K., Mountford, J., Milligan, G., and Baker, A. H. (2013) MicroRNA regulation of endothelial homeostasis and commitment—implications for vascular regeneration strategies using stem cell therapies. Free Radical Biology and Medicine, 64 . pp. 52-60. ISSN 0891-5849 (doi:10.1016/j.freeradbiomed.2013.04.037)

Hudson, B.D., Murdoch, H., and Milligan, G. (2013) The effects of species ortholog and SNP variation on receptors for free fatty acids. Molecular Endocrinology, 27 (8). pp. 1177-1187. ISSN 0888-8809 (doi:10.1210/me.2013-1085)

Milligan, G. (2013) The prevalence, maintenance, and relevance of G protein-coupled receptor oligomerization. Molecular Pharmacology, 84 (1). pp. 158-169. ISSN 0026-895X (doi:10.1124/mol.113.084780)

Hudson, B.D. et al. (2013) Defining the molecular basis for the first potent and selective orthosteric agonists of the FFA2 free fatty acid receptor. Journal of Biological Chemistry, 288 (24). pp. 17296-17312. ISSN 0021-9258 (doi:10.1074/jbc.M113.455337)

Christiansen, E. et al. (2013) Discovery of TUG-770: a highly potent free fatty acid receptor 1 (FFA1/GPR40) agonist for treatment of type 2 diabetes. ACS Medicinal Chemistry Letters, 4 (5). pp. 441-445. ISSN 1948-5875 (doi:10.1021/ml4000673)

Neetoo-Isseljee, Z., MacKenzie, A.E., Southern, C., Jerman, J., McIver, E. G., Harries, N., Taylor, D. L., and Milligan, G. (2013) High-throughput identification and characterization of novel, species-selective GPR35 agonists. Journal of Pharmacology and Experimental Therapeutics, 344 (3). pp. 568-578. ISSN 1521-0103 (doi:10.1124/jpet.112.201798)

Christiansen, E. et al. (2013) Discovery of a potent and selective free fatty acid receptor 1 agonist with low lipophilicity and high oral bioavailability. Journal of Medicinal Chemistry, 56 (3). pp. 982-992. ISSN 0022-2623 (doi:10.1021/jm301470a)

Clarke, C., Flores-Muñoz, M., McKinney, C. A., Milligan, G., and Nicklin, S. A. (2013) Regulation of cardiovascular remodeling by the counter-regulatory axis of the renin–angiotensin system. Future Cardiology, 9 (1). pp. 23-38. ISSN 1479-6678 (doi:10.2217/fca.12.75)

Howard, L., Mackenzie, R. M., Pchelintsev, N. A., McBryan, T., McClure, J. D., McBride, M. W., Kane, N. M., Adams, P. D., Milligan, G., and Baker, A. H. (2013) Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment. Stem Cell Research and Therapy, 4 (2). Art. 36. ISSN 1757-6512 (doi:10.1186/scrt192)

Hudson, B. D., Ulven, T., and Milligan, G. (2013) The therapeutic potential of allosteric ligands for free fatty acid sensitive GPCRs. Current Topics in Medicinal Chemistry, 13 (1). pp. 14-25. ISSN 1568-0266 (doi:10.2174/1568026611313010004)

Jenkins, L., Harries, N., Lappin, J.E., MacKenzie, A.E., Neetoo-Isseljee, Z., Southern, C., McIver, E.G., Nicklin, S.A., Taylor, D.L., and Milligan, G. (2013) Antagonists of GPR35 display high species ortholog selectivity and varying modes of action. Journal of Pharmacology and Experimental Therapeutics, 343 (3). pp. 683-695. ISSN 0022-3565 (doi:10.1124/jpet.112.198945)

Patowary, S., Alvarez-Curto, E., Xu, T.-R., Holz, J.D., Oliver, J.A., Milligan, G., and Raicu, V. (2013) The muscarinic M3acetylcholine receptor exists as two differently sized complexes at the plasma membrane. Biochemical Journal, 452 (2). pp. 303-312. ISSN 0264-6021 (doi:10.1042/BJ20121902)

Segura, V., Pérez-Aso, M., Montó, F., Carceller, E., Noguera, M.A., Pediani, J., Milligan, G., McGrath, I.C., and D’Ocon, P. (2013) Differences in the signaling pathways of α1A- and α1B-adrenoceptors are related to different endosomal targeting. PLoS ONE, 8 (5). e64996. ISSN 1932-6203 (doi:10.1371/journal.pone.0064996)

Ward, R.W., Xu, T.-X., and Milligan, G. (2013) GPCR oligomerization and receptor trafficking. Methods in Enzymology, 521 . pp. 69-90. ISSN 0076-6879 (doi:10.1016/B978-0-12-391862-8.00004-1)

2012

Moreno, J.L. et al. (2012) Identification of three residues essential for 5-hydroxytryptamine 2A-metabotropic glutamate 2 (5-HT2A{middle dot}mGlu2) receptor heteromerization and its psychoactive behavioral function. Journal of Biological Chemistry, 287 (53). pp. 44301-44319. ISSN 0021-9258 (doi:10.1074/jbc.M112.413161)

Hudson, B., Christiansen, E., Tikhonova, I.G., Grundmann, M., Kostenis, E., Adams, D.R., Ulven, T., and Milligan, G. (2012) Chemically engineering ligand selectivity at the free fatty acid receptor 2 based on pharmacological variation between species orthologs. FASEB Journal, 26 (12). pp. 4951-4965. ISSN 0892-6638 (doi:10.1096/fj.12-213314 )

Hudson, B.D., Tikhonova, I.G., Pandey, S.K., Ulven, T., and Milligan, G. (2012) Extracellular ionic locks determine variation in constitutive activity and ligand potency between species orthologs of the free fatty acid receptors FFA2 and FFA3. Journal of Biological Chemistry, 287 (49). pp. 41195-41209. ISSN 0021-9258 (doi:10.1074/jbc.M112.396259)

Christiansen, E. et al. (2012) Free fatty acid receptor 1 (FFA1/GPR40) agonists: mesylpropoxy appendage lowers lipophilicity and improves ADME properties. Journal of Medicinal Chemistry, 55 (14). pp. 6624-6628. ISSN 0022-2623 (doi:10.1021/jm3002026)

Cunningham, M. R., McIntosh, K. A., Pediani, J. D., Robben, J., Cooke, A. E., Nilsson, M., Gould, G. W., Mundell, S., Milligan, G., and Plevin, R. (2012) Novel role for proteinase-activated receptor 2 (PAR2) in membrane trafficking of proteinase-activated receptor 4 (PAR4). Journal of Biological Chemistry, 287 (20). pp. 16656-16669. ISSN 0021-9258 (doi:10.1074/jbc.M111.315911)

Shimpukade, B., Hudson, B.D., Hogaard, C.K., Milligan, G., and Ulven, T. (2012) Discovery of a potent and selective GPR120 agonist. Journal of Medicinal Chemistry, 55 (9). pp. 4511-4515. ISSN 0022-2623 (doi:10.1021/jm300215x)

Xu, T.-R., Ward, R. J., Pediani, J. D., and Milligan, G. (2012) Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation. Journal of Biological Chemistry, 287 . pp. 14937-14949. ISSN 0021-9258 (doi:10.1074/jbc.M111.334300)

Kane, N. M. et al. (2012) Role of MicroRNAs 99b, 181a, and 181b in the differentiation of human embryonic stem cells to vascular endothelial cells. Stem Cells, 30 (4). pp. 643-654. ISSN 1066-5099 (doi:10.1002/stem.1026)

White, K., Kane, N. M., Milligan, G., and Baker, A. H. (2012) The role of miRNA in stem cell pluripotency and commitment to the vascular endothelial lineage. Microcirculation, 19 (3). pp. 196-207. ISSN 1073-9688 (doi:10.1111/j.1549-8719.2012.00161.x)

Xu, T.-R., Lu, R.-F., Romano, D., Pitt, A., Houslay, M. D., Milligan, G., and Kolch, W. (2012) Eukaryotic translation initiation factor 3, subunit a, regulates the extracellular signal-regulated kinase pathway. Molecular and Cellular Biology, 32 (1). pp. 88-95. ISSN 0270-7306 (doi:10.1128/​MCB.05770-11)

Flores, M., Graham, D., Dominiczak, A.F., Milligan, G., Baker, A.H., and Nicklin, S.A. (2012) Angiotensin-(1-9) antagonises Angiotensin II-induced cardiac remodeling via the angiotensin type 2 receptor. Proceedings of the Physiological Society, 27 . C1. ISSN 1749-6187

Flores, M., Graham, D., Dominiczak, A. F., Milligan, G., Baker, A. H., and Nicklin, S. A. (2012) Abstract 231: angiotensin-(1-9) antagonises cardiac remodelling in a mouse model of Angiotensin Ii-induced hypertension. Hypertension, 60 . A231. ISSN 0194-911X

Georgoussi, Z., Georganta, E.M., and Milligan, G. (2012) The other side of opioid receptor signalling: regulation by protein-protein interaction. Current Drug Targets, 13 (1). pp. 80-102. ISSN 1389-4501 (doi:10.2174/138945012798868470)

Laarman, A.J. et al. (2012) Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO Journal, 31 (17). pp. 3607-3619. ISSN 0261-4189 (doi:10.1038/emboj.2012.212)

Pou, C., Mannoury la Cour, C., Stoddart, L.A., Millan, M.J., and Milligan, G. (2012) Functional homomers and heteromers of dopamine D2L and D3 receptors co-exist at the cell surface. Journal of Biological Chemistry, 287 (12). pp. 8864-8878. ISSN 0021-9258 (doi:10.1074/jbc.M111.326678)

Terhzaz, S., Cabrero, P., Robben, J.H., Radford, J.C., Hudson, B.D., Milligan, G., Dow, J.A.T., and Davies, S.A. (2012) Mechanism and function of drosophila capa GPCR: a desiccation stress-responsive receptor with functional homology to human neuromedinU receptor. PLoS ONE, 7 (1). e29897. ISSN 1932-6203 (doi:10.1371/journal.pone.0029897)

2011

Alvarez-Curto, E., Prihandoko, R., Tautermann, C. S. , Zwier, J. M., Pediani, J. D., Lohse, M. J., Hoffmann, C., Tobin, A. B., and Milligan, G. (2011) Developing chemical genetic approaches to explore G protein-coupled receptor function: validation of the use of a Receptor Activated Solely by Synthetic Ligand (RASSL). Molecular Pharmacology, 80 (6). pp. 1033-1046. ISSN 0026-895X (doi:10.1124/mol.111.074674)

Ward, R. J., Pediani, J. D., and Milligan, G. (2011) Heteromultimerization of cannabinoid CB1 receptor and orexin OX1 receptor generates a unique complex in which both protomers are regulated by orexin A. Journal of Biological Chemistry, 286 (43). pp. 37414-37428. ISSN 0021-9258 (doi:10.1074/jbc.M111.287649)

Howard, L., Kane, N., Milligan, G., and Baker, A. H. (2011) MicroRNAs regulating cell pluripotency and vascular differentiation. Vascular Pharmacology, 55 (4). pp. 69-78. ISSN 1537-1891 (doi:10.1016/j.vph.2011.08.002)

Xu, T.‑R. , Ward, R. J., Pediani, J. D., and Milligan, G. (2011) The orexin OX1 receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands. Biochemical Journal, 439 (1). pp. 171-183. ISSN 0264-6021 (doi:10.1042/BJ20110230)

Van Lith, M., Tiwari, S., Pediani, J., Milligan, G., and Bulleid, N. J. (2011) Real-time monitoring of redox changes in the mammalian endoplasmic reticulum. Journal of Cell Science, 124 (14). pp. 2349-2356. ISSN 0021-9533 (doi:10.1242/jcs.085530)

Smith, N.J. et al. (2011) Extracellular loop 2 of the free fatty acid receptor 2 mediates allosterism of a phenylacetamide ago-allosteric modulator. Molecular Pharmacology, 80 (1). pp. 163-173. ISSN 0026-895X (doi:10.1124/mol.110.070789)

Schmidt, J. et al. (2011) Selective orthosteric free fatty acid receptor 2 (FFA2) agonists: identification of the structural and chemical requirements for selective activation of FFA2 versus FFA3. Journal of Biological Chemistry, 286 (12). pp. 10628-10640. ISSN 0021-9258 (doi:10.1074/jbc.M110.210872)

Anthony, D.F., Sin, Y.Y., Vadrevu, S., Advant, N., Day, J.P., Byrne, A.M., Lynch, M.J., Milligan, G., Houslay, M.D., and Baillie, G.S. (2011) β Arrestin 1 inhibits the GAP function of ARHGAP21 so as to promote the activation of RhoA following angiotensin II type 1A receptor stimulation. Molecular and Cellular Biology, 31 (5). pp. 1066-1075. ISSN 0270-7306 (doi:10.1128/MCB.00883-10)

Ward, R. J., Pediani, J. D., and Milligan, G. (2011) Ligand-induced internalisation of the orexin OX1 and cannabinoid CB1 receptors assessed via N-terminal SNAP and CLIP-tagging. British Journal of Pharmacology, 162 (6). pp. 1439-1452. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2010.01156.x)

Ward, R. J., Pediani, J. D., and Milligan, G. (2011) Ligand-induced internalization of the orexin OX1 and cannabinoid CB1 receptors assessed via N-terminal SNAP and CLIP-tagging. British Journal of Pharmacology, 162 (6). pp. 1439-1452. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2010.01156.x)

Flores-Munoz, M., Smith, N. J., Haggerty, C., Milligan, G.,, and Nicklin, S. A. (2011) Angiotensin1-9 antagonises pro-hypertrophic signalling in cardiomyocytes via the angiotensin type 2 receptor. Journal of Physiology, 589 (4). pp. 939-951. ISSN 0022-3751 (doi:10.1113/jphysiol.2010.203075)

Smith, N.J., Bennett, K., and Milligan, G. (2011) When simple agonism is not enough: Emerging modalities of GPCR ligands. Molecular and Cellular Endocrinology, 331 (2). pp. 241-247. ISSN 0303-7207 (doi:10.1016/j.mce.2010.07.009)

Dimond, P. et al. (2011) G protein-coupled receptor modulation with pepducins: moving closer to the clinic. Annals of the New York Academy of Sciences, 1226 (1). pp. 34-49. ISSN 0077-8923 (doi:10.1111/j.1749-6632.2011.06039.x)

Hudson, B.D., Smith, N.J., and Milligan, G. (2011) Experimental challenges to targeting poorly characterized GPCRs: uncovering the therapeutic potential for free fatty acid receptors. In: Neubig, R.R. (ed.) Pharmacology of G Protein Coupled Receptors. Series: Advances in Pharmacology (62). Elsevier Academic Press, Amsterdam, The Netherlands, pp. 175-218. ISBN 9780123859525

Milligan, G. (2011) Orthologue selectivity and ligand bias: translating the pharmacology of GPR35. Trends in Pharmacological Sciences, 32 (5). pp. 317-325. ISSN 0165-6147 (doi:10.1016/j.tips.2011.02.002)

MX PA/A/2005/012546 (2011) Materials and Methods Relating to G-Protein Coupled Oligomers. .

US 10/555,709 (2011) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

US 12/119,401 (2011) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

CN 200480017258.7 (2011) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

TW 093114280 (2011) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

US 09/913, 762 (2011) Receptor Assay. .

Nejsum, L.N., Christensen, T.M., Robben, J.H., Milligan, G., Deen, P.M.T., Bichet, D.G., and Levin, K. (2011) Novel mutation in the AVPR2 gene in a Danish male with nephrogenic diabetes insipidus caused by ER retention and subsequent lysosomal degradation of the mutant receptor. NDT Plus, 4 (3). pp. 158-163. ISSN 1753-0784 (doi:10.1093/ndtplus/sfr010)

Saenz del Burgo, L., and Milligan, G. (2011) Ligand regulation of GPCR quaternary structure. In: Giraldo, J. and Pin, J.-P. (eds.) G Protein-Coupled Receptors: From Structure to Function G Protein-Coupled Receptors: From Structure to Function. Series: RSC drug discovery (8). RSC Publishing, Cambridge, UK, pp. 111-152. ISBN 9781849733441

Schröder, R. et al. (2011) Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells. Nature Protocols, 6 (11). pp. 1748-1760. ISSN 1754-2189 (doi:10.1038/nprot.2011.386)

Tarrasón, G. et al. (2011) The sphingosine-1-phosphate receptor-1 antagonist, W146, causes early and short-lasting peripheral blood lymphopenia in mice. International Immunopharmacology, 11 (11). pp. 1773-1779. ISSN 1567-5769 (doi:10.1016/j.intimp.2011.07.004)

Ward, R.J., Alvarez-Curto, E., and Milligan, G. (2011) Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression. Methods in Molecular Biology, 746 . pp. 21-37. ISSN 1064-3745 (doi:10.1007/978-1-61779-126-0_2)

2010

Burton, P. et al. (2010) Erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) blocks differentiation and maintains the expression of pluripotency markers in human embryonic stem cells. Biochemical Journal, 432 (3). pp. 575-584. ISSN 0264-6021 (doi:10.1042/BJ20100726)

Kane, N. M. et al. (2010) Lentivirus-mediated reprogramming of somatic cells in the absence of transgenic transcription factors. Molecular Therapy, 18 (12). pp. 2139-2145. ISSN 1525-0016 (doi:10.1038/mt.2010.231)

Smith, N. J., and Milligan, G. (2010) Allostery at G protein-coupled receptor Homo- and Heteromers: uncharted pharmacological landscapes. Pharmacological Reviews, 62 (4). pp. 701-725. ISSN 0031-6997 (doi:10.1124/pr.110.002667)

Lopez-Gimenez, J.F., and Milligan, G. (2010) Opioid regulation of Mu receptor internalisation: relevance to the development of tolerance and dependence. CNS and Neurological Disorders: Drug Targets, 9 (5). pp. 616-626. ISSN 1871-5273 (doi:10.2174/187152710793361522)

Alvarez-Curto, E., Pediani, J. D., and Milligan, G. (2010) Applications of fluorescence and bioluminescence resonance energy transfer to drug discovery at G protein coupled receptors. Analytical and Bioanalytical Chemistry, 398 (1). pp. 167-180. ISSN 1618-2642 (doi:10.1007/s00216-010-3823-4)

Schroder, R. et al. (2010) Deconvolution of complex G protein-coupled receptor signaling in live cells using dynamic mass redistribution measurements. Nature Biotechnology, 28 (9). pp. 943-949. ISSN 1087-0156 (doi:10.1038/nbt.1671)

Burton, P. et al. (2010) Identification and characterization of small-molecule ligands that maintain pluripotency of human embryonic stem cells. Biochemical Society Transactions, 38 (4). pp. 1058-1061. ISSN 0300-5127 (doi:10.1042/BST0381058)

Alvarez-Curto, E., Ward, R. J., Pediani, J. D., and Milligan, G. (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry, 285 (30). pp. 23318-23330. ISSN 0021-9258 (doi:10.1074/jbc.M110.122184)

Khelashvili, G. et al. (2010) GPCR-OKB: the G protein coupled receptor oligomer knowledge base. Bioinformatics, 26 (14). pp. 1804-1805. ISSN 1367-4803 (doi:10.1093/bioinformatics/btq264)

Kane, N. M., Meloni, M. , Spencer, H. L., Craig, M. A. , Strehl, R., Milligan, G., Houslay, M. D., Mountford, J. C., Emanueli, C., and Baker, A. H. (2010) Derivation of endothelial cells from human embryonic stem cells by directed differentiation: analysis of microRNA and angiogenesis in vitro and in vivo. Arteriosclerosis, Thrombosis and Vascular Biology, 30 (7). pp. 1389-1397. ISSN 1079-5642 (doi:10.1161/ATVBAHA.110.204800)

del Burgo, L. S., and Milligan, G. (2010) Heterodimerisation of G protein-coupled receptors: implications for drug design and ligand screening. Expert Opinion on Drug Discovery, 5 (5). pp. 461-474. ISSN 1746-0441 (doi:10.1517/17460441003720467)

PA/A/2005/012546 (2010) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

Milligan, G. (2010) The role of dimerisation in the cellular trafficking of G-protein-coupled receptors. Current Opinion in Pharmacology, 10 (1). pp. 23-29. ISSN 1471-4892 (doi:10.1016/j.coph.2009.09.010)

Andrews, P. et al. (2010) High content screening of feeder-free human embryonic stem cells to identify pro-survival small molecules. Biochemical Journal, 432 (Pt 1). pp. 21-33. ISSN 0264-6021 (doi:10.1042/BJ20101022)

Burton, P. et al. (2010) Identification and characterization of small-molecule ligands that maintain pluripotency of human embryonic stem cells. Biochemical Society Transactions, 38 (4). pp. 1058-1061. ISSN 0300-5127 (doi:10.1042/BST0381058)

Jenkins, L., Alvarez-Curto, E., Campbell, K., De Munnik, S., Canals Buj, M., Schlyer, S., and Milligan, G. (2010) Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Gα13 and β-arrestin-2. British Journal of Pharmacology, 162 (3). pp. 733-748. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2010.01082.x)

Jenkins, L., Brea, J., Smith, N.J., Hudson, B.D., Reilly, G., Bryant, N.J., Castro, M., Loza, M.I., and Milligan, G. (2010) Identification of novel species-selective agonists of the G-protein-coupled receptor GPR35 that promote recruitment of β-arrestin-2 and activate Gα13. Biochemical Journal, 432 (3). pp. 451-459. ISSN 0264-6021 (doi:10.1042/BJ20101287)

Stoddart, L.A., and Milligan, G. (2010) Constitutive activity of GPR40/FFA1 intrinsic or assay dependent? Methods in Enzymology, 484 . pp. 569-590. ISSN 0076-6879 (doi:10.1016/B978-0-12-381298-8.00028-9)

Xu, T. et al. (2010) Inferring signaling pathway topologies from multiple perturbation measurements of specific biochemical species. Science Signaling, 3 (113). ra20. ISSN 1945-0877 (doi:10.1126/scisignal.2000517)

2009

Robben, J.H., Fenton, R.A., Vargas, S.L., Schweer, H., Peti-Peterdi, J., Deen, P.M.T., and Milligan, G. (2009) Localization of the succinate receptor in the distal nephron and its signaling in polarized MDCK cells. Kidney International, 76 (12). pp. 1258-1267. ISSN 0085-2538 (doi:10.1038/ki.2009.360)

Milligan, G. (2009) G protein-coupled receptor hetero-dimerization: contribution to pharmacology and function. British Journal of Pharmacology, 158 (1). pp. 5-14. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2009.00169.x)

Milligan, G., and McGrath, J.C. (2009) GPCR theme editorial. British Journal of Pharmacology, 158 (1). pp. 1-4. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2009.00422.x)

Robben, J.H., Kortenoeven, M.L.A., Sze, M., Yae, C., Milligan, G., Oorschot, V.M., Klumperman, J., Knoers, N.V.A.M., and Deen, P.M.T. (2009) Intracellular activation of vasopressin V2 receptor mutants in nephrogenic diabetes insipidus by nonpeptide agonists. Proceedings of the National Academy of Sciences of the United States of America, 106 (29). pp. 12195-12200. ISSN 0027-8424 (doi:10.1073/pnas.0900130106)

Smith, N.J., Stoddart, L.A., Devine, N.M., Jenkins, L., and Milligan, G. (2009) The action and mode of binding of thiazolidinedione ligands at free fatty acid receptor 1. Journal of Biological Chemistry, 284 (26). pp. 17527-17539. ISSN 0021-9258 (doi:10.1074/jbc.M109.012849)

Idris, A.I., Sophocleous, A., Landao-Bassonga, E., Merkouris, M., Milligan, G., Baker, D., van't Hof, R.J., and Ralston, S.H. (2009) Cannabinoid receptor type 1 protects against age-related bone loss by regulating osteoblast and adipocyte differentiation of bone marrow stromal cells. Bone, 44 (Supple). S222. ISSN 8756-3282 (doi:10.1016/j.bone.2009.03.067)

Ward, R. J., Jenkins, L., and Milligan, G. (2009) Selectivity and functional consequences of interactions of family A G protein-coupled receptors with neurochondrin and periplakin. Journal of Neurochemistry, 109 (1). pp. 182-192. ISSN 0022-3042 (doi:10.1111/j.1471-4159.2009.05918.x)

Ferre, S. et al. (2009) Building a new conceptual framework for receptor heteromers. Nature Chemical Biology, 5 (3). pp. 131-134. ISSN 1552-4450 (doi:10.1038/nchembio0309-131)

Canals, M., Lopez-Gimenez, J. F. , and Milligan, G. (2009) Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective α1b-adrenoceptor mutant demonstrates membrane targeting of GPCR oligomers. Biochemical Journal, 417 (1). pp. 161-172. ISSN 0264-6021 (doi:10.1042/BJ20081227)

Appelbe, S., and Milligan, G. (2009) Hetero‐Oligomerization of Chemokine Receptors. In: Chemokines, Part B. Series: Methods in Enzymology. , 461 . Academic Press, pp. 207-225. ISBN 9780123749079

Bennett, K.A., Langmead, C.J., Wise, A., and Milligan, G. (2009) Growth hormone secretagogues and growth hormone releasing peptides act as orthosteric super-agonists but not allosteric regulators for activation of the G protein G{alpha}o1 by the ghrelin receptor. Molecular Pharmacology, 76 (4). pp. 802-811. ISSN 0026-895X (doi:10.1124/mol.109.056101)

Brea, J.H. et al. (2009) Evidence for distinct antagonist-revealed functional states of 5-HT2A receptor homodimers. Molecular Pharmacology, 75 (6). pp. 1380-1391. ISSN 0026-895X (doi:10.1124/mol.108.054395)

Idris, A. I., Sophocleous, A., Landa-Bassonga, E., Canals, M., Milligan, G., Baker, D., van't Hof, R. J., and Ralston, S. (2009) Cannabinoid receptor type 1 protects against age-related bone loss by regulating osteoblast and adipocyte differentiation of bone marrow stromal cells. Cell Metabolism, 10 (2). pp. 139-147. ISSN 1550-4131 (doi:10.1016/j.cmet.2009.07.006)

Milligan, G., Stoddart, L.A., and Smith, N.J. (2009) Agonism and allosterism: the pharmacology of the free fatty acid receptors FFA2 and FFA3. British Journal of Pharmacology, 158 (1). pp. 146-153. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2009.00421.x)

2008

Lopez-Gimenez, J. F., Milligan, G., and Vilaro, M. T. (2008) Morphine desensitization, internalization, and down-regulation of the μ opioid receptor is facilitated by serotonin 5-hydroxytryptamine2A receptor coactivation. Molecular Pharmacology, 74 (5). pp. 1278-1291. ISSN 0026-895X (doi:10.1124/mol.108.048272)

Lopez-Gimenez, J. F., Vilaro, M. T., and Milligan, G. (2008) Morphine desensitization, internalization and down-regulation of the mu opioid receptor is facilitated by serotonin 5-HT2A receptor co-activation. Molecular Pharmacology, 74 (5). pp. 1278-1291. ISSN 0026-895X (doi:10.1124/mol.108.048272)

10/555709 (2008) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

Xu, T.-R., Baillie, G. S., Bhari, N., Houslay, T. M., Pitt, A. M., Adams, D. R., Kolch, W., Houslay, M. D., and Milligan, G. (2008) Mutations of β-arrestin 2 that limit self-association also interfere with interactions with the β2-adrenoceptor and the ERK1/2 MAPKs: implications for β2-adrenoceptor signalling via the ERK1/2 MAPKs. Biochemical Journal, 413 (1). pp. 51-60. ISSN 0264-6021 (doi:10.1042/BJ20080685)

Lane, J.R., Henderson, D., Powney, B., Wise, A., Rees, S., Daniels, D., Plumpton, C., Kinghorn, I., and Milligan, G. (2008) Antibodies that identify only the active conformation of Gi family G protein (alpha) subunits. FASEB Journal, 22 (6). pp. 1924-1932. ISSN 0892-6638 (doi:10.1096/fj.07-100388)

Canals Buj, M., and Milligan, G. (2008) Constitutive activity of the cannabinoid CB1 receptor regulates the function of co-expressed Mu opioid receptors. Journal of Biological Chemistry, 283 (17). pp. 11424-11434. ISSN 0021-9258 (doi:10.1074/jbc.M710300200)

González-Maeso, J. et al. (2008) Identification of a serotonin/glutamate receptor complex implicated in psychosis. Nature, 452 (7183). pp. 93-97. ISSN 0028-0836 (doi:10.1038/nature06612)

Milligan, G. (2008) A day in the life of a G protein-coupled receptor: the contribution to function of G protein-coupled receptor dimerization. British Journal of Pharmacology, 153 (.). S216-S229. ISSN 0007-1188 (doi:10.1038/sj.bjp.0707490)

Hill, C., Brownlie, Z., Davey, J., Milligan, G., and Ladds, G. (2008) Isolation and characterization of a novel human RGS mutant displaying gain-of-function activity. Cellular Signalling, 20 (2). pp. 323-336. ISSN 0898-6568 (doi:10.1016/j.cellsig.2007.10.016)

Lane, J.R., Powney, B., Wise, A., Rees, S., and Milligan, G. (2008) G protein-coupling and ligand selectivity of the D2L and D3 dopamine receptors. Journal of Pharmacology and Experimental Therapeutics, 325 (1). pp. 319-330. ISSN 0022-3565 (doi:10.1124/jpet.107.134296)

Lane, J.R., Henderson, D., Powney, B., Wise, A., Rees, S., Daniels, D., Plumpton, C., Kinghorn, I., and Milligan, G. (2008) Antibodies that identify only the active conformation of Gi family G protein α subunits. FASEB Journal, 22 . pp. 1924-1932. ISSN 0892-6638 (doi:10.1096/fj.07-100388)

Lane, J.R., Powney, B., Wise, A., Rees, S., and Milligan, G. (2008) G protein-coupling and ligand selectivity of the D2L and D3 dopamine receptors. Journal of Pharmacology and Experimental Therapeutics, 325 (1). pp. 319-330. ISSN 0022-3565 (doi:10.1124/jpet.107.134296)

McCulloch, C., Morrow, V., Milasta, S., Comerford, I., Milligan, G., Graham, G., Isaacs, N., and Nibbs, R. (2008) Multiple roles for the C-terminal tail of the chemokine scavenger D6. Journal of Biological Chemistry, 283 (12). pp. 7972-7982. ISSN 0021-9258 (doi:10.1074/jbc.M710128200 )

Noubade, R., Saligrama, N., Spach, K., del Rio, R., Blankenhorn, E.P., Kantidakis, T., Milligan, G., Rincon, M., and Teuscher, C. (2008) Autoimmune disease-associated histamine receptor H1 alleles exhibit differential protein trafficking and cell surface expression. Journal of Immunology, 180 (11). pp. 7471-7479. ISSN 0022-1767

Parenty, G., Applebe, S., and Milligan, G. (2008) CXCR2 chemokine receptor antagonism enhances DOP opioid receptor function via allosteric regulation of the CXCR2-DOP receptor hetero-dimer. Biochemical Journal, 412 (Pt2). pp. 215-256. ISSN 0264-6021 (doi:10.1042/BJ20071689)

Snook, L. A., Milligan, G., Kieffer, B. L., and Massotte, D. (2008) Co-expression of mu and delta opioid receptors as receptor-G protein fusions enhances both mu and delta signalling via distinct mechanisms. Journal of Neurochemistry, 105 (3). pp. 865-873. ISSN 0022-3042 (doi:10.1111/j.1471-4159.2008.05215.x)

Stoddart, L. A., Smith, N. J., Jenkins, L., Brown, A. J., and Milligan, G. (2008) Conserved Polar Residues in Transmembrane Domains V, VI, and VII of Free Fatty Acid Receptor 2 and Free Fatty Acid Receptor 3 Are Required for the Binding and Function of Short Chain Fatty Acids. Journal of Biological Chemistry, 283 (47). pp. 32913-32924. ISSN 0021-9258 (doi:10.1074/jbc.M805601200)

Stoddart, L. A., Smith, N. J., and Milligan, G. (2008) International Union of Pharmacology. LXXI. Free Fatty Acid Receptors FFA1, -2, and -3: Pharmacology and Pathophysiological Functions. Pharmacological Reviews, 60 (4). pp. 405-417. ISSN 0031-6997 (doi:10.1124/pr.108.00802)

2007

Snook, L.A., Milligan, G., Kieffer, B.L., and Massotte, D. (2007) Co-expression of mu and delta opioid receptors as receptor-G protein fusions enhances both mu and delta signalling via distinct mechanisms. Journal of Neurochemistry, 105 (3). pp. 865-873. ISSN 0022-3042 (doi:10.1111/j.1471-4159.2008.05215.x)

Noubade, R., Milligan, G., Zachary, J.F., Blankenhorn, E.P., del Rio, R., Rincorn, M., and Teuscher, C. (2007) Histamine receptor H1 is required for TCR-mediated p38 MAPK activation and optimal IFN-γ production in mice. Journal of Clinical Investigation, 117 (11). pp. 3507-3518. ISSN 0021-9738

Baillie, G.S. et al. (2007) Mapping binding sites for the PDE4D5 cAMP-specific phosphodiesterase to the N- and C-domains of beta-arrestin using spot-immobilized peptide arrays. Biochemical Journal, 404 (1). pp. 71-80. ISSN 0264-6021 (doi:10.1042%2FBJ20070005)

Skrabanek, L. et al. (2007) Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base. BMC Bioinformatics, 8 (177). ISSN 1471-2105 (doi:10.1186/1471-2105-8-177)

Lopez-Gimenez, J. F., Canals Buj, M. , Pediani, J. D., and Milligan, G. (2007) The 1b-adrenoceptor exists as a higher-order oligomer: effective oligomerization is required for receptor maturation, surface delivery, and function. Molecular Pharmacology, 71 (4). pp. 1015-1029. ISSN 0026-895X (doi:10.1124/mol.106.033035)

Milligan, G. (2007) G protein-coupled receptor dimerisation: molecular basis and relevance to function. Biochimica et Biophysica Acta: Biomembranes, 1768 (4). pp. 825-835. ISSN 0005-2736 (doi:10.1016/j.bbamem.2006.09.021)

Bouvier, M., Heveker, N., Jockers, R., Marullo, S., and Milligan, G. (2007) BRET analysis of GPCR oligomerization: newer does not mean better. Nature Methods, 4 (1). pp. 3-4. ISSN 1548-7091 (doi:10.1038/nmeth0107-3)

Fredholm, B., Hokfelt, T., and Milligan, G. (2007) G-protein-coupled receptors: an update. Acta Physiologica, 190 . pp. 3-7. (doi:10.1111/j.1748-1716.2007.01689.x)

Jameson, E., Pei, J., Wade, S., Neubig, R., Milligan, G., and Kennedy, R. (2007) Capillary electrophoresis assay for G protein-coupled receptor-mediated GTPase activity. Analytical Chemistry, 79 . pp. 1158-1163. (doi:10.1021/ac061099g)

Lane, J., Powney, B., Wise, A., Rees, S., and Milligan, G. (2007) Protean agonism at the dopamine D-2 receptor: (S)-3-(3-hydroxyphenyl)-N-propylpiperidine is an agonist for activation of G(o1) but an antagonist/inverse agonist for G(i1), G(i2), and G(i3). Molecular Pharmacology, 71 . pp. 1349-1359. (doi:10.1124/mol.106.032722)

Milligan, G. (2007) Allosteric modulation of heterodimeric G-protein-coupled receptors. Trends in Pharmacological Sciences, 28 (12). pp. 615-620. ISSN 0165-6147 (doi:10.1016/j.tips.2007.11.001)

Milligan, G., Parenty, G., Stoddart, L., and Lane, J. (2007) Novel pharmacological applications of G-protein-coupled receptor-G protein fusions. Current Opinion in Pharmacology, 7 . pp. 521-526. (doi:10.1016/j.coph.2007.06.007)

Milligan, G., and Vanderbauwhede, W. (2007) Implementation of finite state machines on a reconfigurable device. In: Arslan, T. (ed.) Second NASA/ESA Conference on Adaptive Hardware and Systems (AHS 2007), 5-8 Aug. 2007, Edinburgh, UK. IEEE Computer Society, Los Alamitos, USA, pp. 386-396. ISBN 9780769528663

Noubade, R., Milligan, G., Zachary, J., Blankenhorn, E., del Rio, R., Rincon, M., and Teuscherl, C. (2007) Histamine receptor H-1 is-required for TCR-mediated p38 MAPK activation and optimal IFN-gamma production in mice. Journal of Clinical Investigation, 117 . pp. 3507-3518. (doi:10.1172/JC132792)

Orth, J., Lang, S., Preuss, I., Milligan, G., and Aktories, K. (2007) Action of Pasteurella multocida toxin on G alpha(q) is persistent and independent of interaction with G-protein-coupled receptors. Cellular Signalling, 19 . pp. 2174-2182. (doi:10.1016/j.cellsig.2007.06.016)

Pin, J. et al. (2007) International union of basic and clinical pharmacology. LXVII. Recommendations for the recognition and nomenclature of g protein-coupled receptor heteromultimers. Pharmacological Reviews, 59 . pp. 5-13. (doi:10.1124/pr.59.1.5)

Sartania, N., Appelbe, S., Pediani, J. D., and Milligan, G. (2007) Agonist occupancy of a single monomeric element is sufficient to cause internalization of the dimeric beta(2)-adrenoceptor. Cellular Signalling, 19 . pp. 1928-1938. ISSN 0898-6568 (doi:10.1016/j.cellsig.2007.05.002)

Stoddart, L., Brown, A., and Milligan, G. (2007) Uncovering the pharmacology of the G protein-coupled receptor GPR40: High apparent constitutive activity in guanosine 5'-O-(3-[S-35] thio) triphosphate binding studies reflects binding of an endogenous agonist. Molecular Pharmacology, 71 . pp. 994-1005. (doi:10.1124/mol.106.031534)

2006

Ellis, J., Pediani, J.D., Canals Buj, M., Milasta, S., and Milligan, G. (2006) Orexin-1 receptor-cannabinoid CB1 receptor heterodimerization results in both ligand-dependent and -independent coordinated alterations of receptor localization and function. Journal of Biological Chemistry, 281 (25). pp. 38812-38824. ISSN 0021-9258 (doi:10.1074/jbc.M602494200)

Comerford, I., Milasta, S., Morrow, V., Milligan, G., and Nibbs, R. (2006) The chemokine receptor CCX-CKR mediates effective scavenging of CCL19 in vitro. European Journal of Immunology, 36 (7). pp. 1904-1916. ISSN 0014-2980 (doi:10.1002/eji.200535716)

Batenburg, W. et al. (2006) Carvedilol-induced antagonism of angiotensin II: a matter of alpha(1)-adrenoceptor blockade. Journal of Hypertension, 24 . pp. 1355-1363.

Canals, M., Jenkins, L., Kellett, E., and Milligan, G. (2006) Up-regulation of the angiotensin II type 1 receptor by the MAS proto-oncogene is due to constitutive activation of G(q)/G(11) by MAS. Journal of Biological Chemistry, 281 . pp. 16757-16767. ISSN 0021-9258 (doi:10.1074/jbc.M601121200)

Ciruela, F. et al. (2006) Presynaptic control of striatal glutamatergic neurotransmission by adenosine A(1)-A(2A) receptor heteromers. Journal of Neuroscience, 26 . pp. 2080-2087. (doi:10.1523/JNEUROSCI.3574-05.2006)

Francke, F., Ward, R., Jenkins, L., Kellett, E., Richter, D., Milligan, G., and Bachner, D. (2006) Interaction of neurochondrin with the melanin-concentrating hormone receptor 1 interferes with G protein-coupled signal transduction but not agonist-mediated internalization. Journal of Biological Chemistry, 281 . pp. 32496-32507. ISSN 0021-9258 (doi:10.1074/jbc.M602889200)

Milasta, S., Pediani, J., Appelbe, S., Trim, S., Wyatt, M., Cox, P., Fidock, M., and Milligan, G. (2006) Interactions between the Mas-related receptors MrgD and MrgE alter signalling and trafficking of MrgD. Molecular Pharmacology, 69 . pp. 479-491. (doi:10.1124/mol.105.018788)

Milligan, G. (2006) G-protein-coupled receptor heterodimers: pharmacology, function and relevance to drug discovery. Drug Discovery Today, 11 . pp. 541-549. (doi:10.1016/j.drudis.2006.04.007)

Milligan, G., and Kostenis, E. (2006) Heterotrimeric G-proteins: a short history. British Journal of Pharmacology, 147 . S46-S55.

Milligan, G., Pediani, J., Canals, M., and Lopez-Gimenez, J. (2006) Oligomeric structure of the alpha(1b)-adrenoceptor: Comparisons with rhodopsin. Vision Research, 46 . pp. 4434-4441. ISSN 0042-6989 (doi:10.1016/j.visres.2006.08.007)

Milligan, G., Stoddart, L., and Brown, A. (2006) G protein-coupled receptors for free fatty acids. Cellular Signalling, 18 . pp. 1360-1365. (doi:10.1016/j.cellsig.2006.03.011)

Novotny, J., Durchankova, D., Ward, R., Carrillo, J., Svoboda, P., and Milligan, G. (2006) Functional interactions between the alpha(1b)-adrenoceptor and G alpha(11) are compromised by de-palmitoylation of the G protein but not of the receptor. Cellular Signalling, 18 . pp. 1244-1251. (doi:10.1016/j.cellsig.2005.10.004)

Snook, L., Milligan, G., Kieffer, B., and Massotte, D. (2006) mu-delta opioid receptor functional interaction: Insight using receptor-G protein fusions. Journal of Pharmacology and Experimental Therapeutics, 318 . pp. 683-690. (doi:10.1124/jpet.106.101220)

2005

Milligan, G., and Bouvier, M. (2005) Methods to monitor the quaternary structure of G protein-coupled receptors. FEBS Journal, 272 (12). pp. 2914-2925. ISSN 1742-464X (doi:10.1111/j.1742-4658.2005.04731.x)

Barclay, E., OReilly, M., and Milligan, G. (2005) Activation of an alpha(2A)-adrenoceptor-G alpha(o1), fusion protein dynamically regulates the palmitoylation status of the G protein but not of the receptor. Biochemical Journal, 385 . pp. 197-206. ISSN 0264-6021

Barnes, W., Reiter, E., Violin, J., Ren, X., Milligan, G., and Lefkowitz, R. (2005) beta-arrestin 1 and G(alpha q/11) coordinately activate RhoA and stress fiber formation following receptor stimulation. Journal of Biological Chemistry, 280 . pp. 8041-8050. ISSN 0021-9258 (doi:10.1074/jbc.M412924200)

Holdsworth, G., Slocombe, P., Hutchinson, G., and Milligan, G. (2005) Analysis of endogenous S1P and LPA receptor expression in CHO-K1 cells. Gene, 350 . pp. 59-63. (doi:10.1016/j.gene.2005.01.016)

Hunton, D., Barnes, W., Kim, J., Ren, X., Violin, J., Reiter, E., Milligan, G., Patel, D., and Lefkowitz, R. (2005) beta-arrestin 2-dependent angiotensin II type 1A receptor-mediated pathway of chemotaxis. Molecular Pharmacology, 67 . pp. 1229-1236. (doi:10.1124/mol.104.006270)

Kostenis, E., Martini, L., Ellis, J., Waldhoer, M., Heydorn, A., Rosenkilde, M., Norregaard, P., Jorgensen, R., Whistler, J., and Milligan, G. (2005) A highly conserved glycine within linker I and the extreme C terminus of G protein alpha subunits interact cooperatively in switching G protein-coupled receptor-to-effector specificity. Journal of Pharmacology and Experimental Therapeutics, 313 . pp. 78-87. (doi:10.1124/jpet.104.080424)

Kostenis, E. et al. (2005) (G)-protein-coupled receptor Mas is a physiological antagonist of the angiotensin II type 1 receptor. Circulation, 111 . pp. 1806-1813. (doi:10.1161/01.CIR.0000160867.23556.7D)

Kostenis, E., Waelbroeck, M., and Milligan, G. (2005) Techniques: Promiscuous G alpha proteins in basic research and drug discovery. Trends in Pharmacological Sciences, 26 . pp. 595-602. ISSN 0165-6147 (doi:10.1016/j.tips.2005.09.007)

Milasta, S., Evans, N., Ormiston, L., Wilson, S., Lefkowitz, R., and Milligan, G. (2005) The sustainability of interactions between the orexin-1 receptor and beta-arrestin-2 is defined by a single C-terminal cluster of hydroxy amino acids and modulates the kinetics of ERK MAPK regulation. Biochemical Journal, 387 . pp. 573-584. ISSN 0264-6021

Milligan, G. (2005) Opioid receptors and their interacting proteins. Neuromolecular Medicine, 7 . pp. 51-59. (doi:10.1385/NMM)

Milligan, G., Wilson, S., and Lopez-Gimenez, J. (2005) The specificity and molecular basis of alpha(1)-adrenoceptor and CXCR chemokine receptor dimerization. Journal of Molecular Neuroscience, 26 . pp. 161-167. (doi:10.1385/JMN/26)

Murdoch, H., Feng, G., Bachner, D., Ormiston, L., White, J., Richter, D., and Milligan, G. (2005) Periplakin interferes with g protein activation by the melanin-concentrating hormone receptor-1 by binding to the proximal segment of the receptor C-terminal tail. Journal of Biological Chemistry, 280 . pp. 8208-8220. ISSN 0021-9258 (doi:10.1074/jbc.M405215200)

Pascal, G., and Milligan, G. (2005) Functional complementation and the analysis of opioid receptor homodimerization. Molecular Pharmacology, 68 . pp. 905-915. (doi:10.1124/mol.105.013847)

Pediani, J., Colston, J., Caldwell, D., Milligan, G., Daly, C., and McGrath, J. (2005) beta-arrestin-dependent spontaneous alpha(1a)-adrenoceptor endocytosis causes intracellular transportation of alpha-blockers via recycling compartments. Molecular Pharmacology, 67 . pp. 992-1004. (doi:10.1124/mol.104.008417)

Rudajev, V., Novotny, J., Hejnova, L., Milligan, G., and Svoboda, P. (2005) Dominant portion of thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and G(q)alpha/G(11)alpha protein. Journal of Biochemistry, 138 . pp. 111-125. (doi:10.1093/jb/mvi114)

Ward, R., and Milligan, G. (2005) A key serine for the GTPase-activating protein function of regulator of G protein signaling proteins is not a general target for 14-3-3 interactions. Molecular Pharmacology, 68 . pp. 1821-1830. (doi:10.1124/moi.105.015073)

Wilson, S., Wilkinson, G., and Milligan, G. (2005) The CXCR1 and CXCR2 receptors form constitutive homo- and heterodimers selectively and with equal apparent affinities. Journal of Biological Chemistry, 280 . pp. 28663-28674. ISSN 0021-9258 (doi:10.1074/jbc.M413475200)

2004

093114280 (2004) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

Milligan, G., Feng, G.J., Ward, R.J., Sartania, N., Ramsay, D., McLean, A.J., and Carrillo, J.J. (2004) G protein-coupled receptor fusion proteins in drug discovery. Current Pharmaceutical Design, 10 (17). pp. 1989-2001. ISSN 1381-6128 (doi:10.2174/1381612043384295)

Bakker, R., Dees, G., Carrillo, J., Booth, R., Lopez-Gimenez, J., Milligan, G., Strange, P., and Leurs, R. (2004) Domain swapping in the human histamine H-1 receptor. Journal of Pharmacology and Experimental Therapeutics, 311 . pp. 131-138. (doi:10.1124/jpet.104.067041)

Carrillo, J., Lopez-Gimenez, J., and Milligan, G. (2004) Multiple interactions between transmembrane helices generate the oligomeric alpha(1b)-adrenoceptor. Molecular Pharmacology, 66 . pp. 1123-1137. (doi:10.1124/mol.104.001586)

Heydorn, A., Ward, R., Jorgensen, R., Rosenkilde, M., Frimurer, T., Milligan, G., and Kostenis, E. (2004) Identification of a novel site within G protein alpha subunits important for specificity of receptor-G protein interaction. Molecular Pharmacology, 66 . pp. 250-259.

Holdsworth, G., Osborne, D.A., Thi Pham, T., Fells, J.I., Hutchinson, G., Milligan, G., and Parrill, A.L. (2004) A single amino acid determines preference between phospholipids and reveals length restriction for activation ofthe S1P4 receptor. BMC Biochemistry, 5 (12). ISSN 1471-2091 (doi:10.1186/1471-2091-5-12)

Klaasse, E., de Ligt, R., Roerink, S., Lorenzen, A., Milligan, G., Leurs, R., and IJzerman, A. (2004) Allosteric modulation and constitutive activity of fusion proteins between the adenosine A(1) receptor and different (351)Cys-mutated G(i) alpha-subunits. European Journal of Pharmacology, 499 . pp. 91-98. (doi:10.1016/j.ejphar.2004.07.108)

Milligan, G. (2004) Applications of bioluminescence- and fluorescence resonance energy transfer to drug discovery at G protein-coupled receptors. European Journal of Pharmaceutical Sciences, 21 . pp. 397-405. (doi:10.1016/j.ejps.2003.11.010)

Milligan, G. (2004) G protein-coupled receptor dimerization: Function and ligand pharmacology. Molecular Pharmacology, 66 . pp. 1-7. (doi:10.1124/mol.104.000497)

Milligan, G. (2004) A single amino acid determines preference between phospholipids and reveals length restriction for activation ofthe S1P4 receptor. BMC Biochemistry, 5 (12). pp. 1-12. ISSN 1471-2091 (doi:10.1186/1471-2091-5-12)

Milligan, G., Lopez-Gimenez, J., Wilson, S., and Carrillo, J. (2004) Selectivity in the oligomerisation of G protein-coupled receptors. Seminars in Cell and Developmental Biology, 15 . pp. 263-268. (doi:10.1016/j.semcdb.2003.12.012)

Milligan, G., Murdoch, H., Kellett, E., White, J., and Feng, G. (2004) Interactions between G-protein-coupled receptors and periplakin: a selective means to regulate G-protein activation. Biochemical Society Transactions, 32 . pp. 878-880.

Milligan, G., Pascal, G., and Carrillo, J. (2004) Monitoring receptor dimerisation. Medicinal Chemistry Research, 13 . pp. 18-24.

Milligan, G., Pediani, J., Fidock, M., and Lopez-Gimenez, J. (2004) Dimerization of alpha(1)-adrenoceptors. Biochemical Society Transactions, 32 . pp. 847-850.

Moravcova, Z., Rudajev, V., Stohr, J., Novotny, J., Cerny, J., Parenti, M., Milligan, G., and Svoboda, P. (2004) Long-term agonist stimulation of IP prostanoid receptor depletes the cognate G(s)alpha protein in membrane domains but does not change the receptor level. Biochimica et Biophysica Acta: Molecular Cell Research, 1691 . pp. 51-65. ISSN 0167-4889 (doi:10.1016/j.bbamcr.2003.12.004)

Ramsay, D., Carr, I., Pediani, J., Lopez-Gimenez, J., Thurlow, R., Fidock, M., and Milligan, G. (2004) High-affinity interactions between human alpha(1A)-adrenoceptor C-terminal splice variants produce homo- and heterodimers but do not generate the alpha(1L)-adrenoceptor. Molecular Pharmacology, 66 . pp. 228-239.

Ward, R.J., and Milligan, G. (2004) Analysis of function of receptor-G-protein and receptor-RGS fusion proteins. Methods in Molecular Biology, 259 . pp. 225-247. ISSN 1064-3745

2003

60/472025 (2003) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

Adie, E., Francis, M., Davies, J., Smith, L., Marenghi, A., Hather, C., Hadingham, K., Michael, N., Milligan, G., and Gamel, S. (2003) CypHer 5: A generic approach for measuring the activation and trafficking of G protein-coupled receptors in live cells. Assay and Drug Development Technologies, 1 . pp. 251-259.

Bahia, D. S., Sartania, N., Ward, R. j., Cavalli, A., Jones, T. L.Z., Druey, K. M., and Milligan, G. (2003) Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity o. Journal of Neurochemistry, 85 (5). pp. 1289-1298. ISSN 0022-3042 (doi:10.1046/j.1471-4159.2003.01769.x)

Benians, A., Leaney, J., Milligan, G., and Tinker, A. (2003) The dynamics of formation and action of the ternary complex revealed in living cells using a G-protein-gated K+ channel as a biosensor. Journal of Biological Chemistry, 278 . pp. 10851-10858. ISSN 0021-9258 (doi:10.1074/jbc.M212299200)

Bertaso, F., Ward, R., Viard, P., Milligan, G., and Dolphin, A. (2003) Mechanism of action of G(q) to inhibit G beta gamma modulation of Ca(v)2.2 calcium channels: Probed by the use of receptor-G alpha tandems. Molecular Pharmacology, 63 . pp. 832-843.

Bourova, L., Kostrnova, A., Hejnova, L., Moravcova, Z., Moon, H., Novotny, J., Milligan, G., and Svoboda, P. (2003) delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains. Journal of Neurochemistry, 85 . pp. 34-49. (doi:10.1046/j.1471-4159.2003.01667.x)

Carrillo, J., Pediani, J., and Milligan, G. (2003) Dimers of class A G protein-coupled receptors function via agonist-mediated trans-activation of associated G proteins. Journal of Biological Chemistry, 278 . pp. 42578-42587. ISSN 0021-9258 (doi:10.1074/jbc.M306165200)

Derrien, A., Zheng, B., Osterhout, J., Ma, Y., Milligan, G., Farquhar, M., and Druey, K. (2003) Src-mediated RGS16 tyrosine phosphorylation promotes RGS16 stability. Journal of Biological Chemistry, 278 . pp. 16107-16116. ISSN 0021-9258 (doi:10.1074/jbc.M210371200)

Feng, G., Kellett, E., Scorer, C., Wilde, J., White, J., and Milligan, G. (2003) Selective interactions between helix VIII of the human mu-opioid receptors and the C terminus of periplakin disrupt G protein activation. Journal of Biological Chemistry, 278 . pp. 33400-33407. ISSN 0021-9258 (doi:10.1074/jbc.M305866200)

Hiol, A., Davey, P., Osterhout, J., Waheed, A., Fischer, E., Chen, C., Milligan, G., Druey, K., and Jones, T. (2003) Palmitoylation regulates regulators of G-protein signaling (RGS) 16 function - I. Mutation of amino-terminal cysteine residues on RGS16 prevents its targeting to lipid rafts and palmitoylation of an internal cysteine residue. Journal of Biological Chemistry, 278 . pp. 19301-19308. ISSN 0021-9258 (doi:10.1074/jbc.M210123200)

Milligan, G. (2003) Constitutive activity and inverse agonists of G protein-coupled receptors: a current perspective. Molecular Pharmacology, 64 . pp. 1271-1276.

Milligan, G. (2003) High-content assays for ligand regulation of G-protein-coupled receptors. Drug Discovery Today, 8 . pp. 579-585.

Milligan, G. (2003) Mechanism of action of Gq to inhibit Gbg modulation of Cav2.2 calcium channls probed by use of receptor-Ga tandems. Molecular Pharmacology, 63 (4). pp. 832-843. (doi:10.1124/mol.63.4.832)

Milligan, G. (2003) Principles: Extending the utility of [S-35]GTP gamma S binding assays. Trends in Pharmacological Sciences, 24 . pp. 87-90. ISSN 0165-6147

Milligan, G., Ramsay, D., Pascal, G., and Carrillo, J. (2003) GPCR dimerisation. Life Sciences, 74 . pp. 181-188. (doi:10.1016/j.lfs.2003.09.005)

Osterhout, J., Waheed, A., Hiol, A., Ward, R., Davey, P., Nini, L., Wang, J., Milligan, G., Jones, T., and Druey, K. (2003) Palmitoylation regulates regulator of G-protein signaling (RGS) 16 function - II. Palmitoylation of a cysteine residue in the RGS box is critical for RGS16 GTPase accelerating activity and regulation of G(i)-coupled signaling. Journal of Biological Chemistry, 278 . pp. 19309-19316. ISSN 0021-9258 (doi:10.1074/jbc.M210124200)

Zeng, F., McLean, A., Milligan, G., Lerner, M., Chalmers, D., and Behan, D. (2003) Ligand specific up-regulation of a Renilla reniformis luciferase-tagged, structurally unstable muscarinic M-3 chimeric G protein-coupled receptor. Molecular Pharmacology, 64 . pp. 1474-1484.

2002

Adamson, P., Wilbourn, B., Etienne-Manneville, S., Calder, V., Beraud, E., Milligan, G., Couraud, P., and Greenwood, J. (2002) Lymphocyte trafficking through the blood-brain barrier is dependent on endothelial cell heterotrimeric G-protein signaling. FASEB Journal, 16 .

Adie, E., Kalinka, S., Smith, L., Francis, M., Marenghi, A., Cooper, M., Briggs, M., Michael, N., Milligan, G., and Game, S. (2002) A pH-sensitive fluor, CypHer (TM) 5, used to monitor agonist-induced g protein-coupled receptor internalization in live cells. Biotechniques, 33 . pp. 1152-1157.

Carrillo, J., Stevens, P., and Milligan, G. (2002) Measurement of agonist-dependent and -independent signal initiation of alpha(1b)-adrenoceptor mutants by direct analysis of guanine nucleotide exchange on the G protein G alpha(11). Journal of Pharmacology and Experimental Therapeutics, 302 . pp. 1080-1088. (doi:10.1124/jpet.102.035501)

Feng, G., Cavalli, A., and Milligan, G. (2002) Engineering a V-2 vasopressin receptor agonist- and regulator of G-protein-signaling-sensitive G protein. Analytical Biochemistry, 300 . pp. 212-220. (doi:10.1006/abio.2001.5448)

Liu, S., Carrillo, J., Pediani, J., and Milligan, G. (2002) Effective information transfer from the alpha(1b)-adrenoceptor to G alpha(11) requires both beta/gamma interactions and an aromatic group four amino acids from the C terminus of the G protein. Journal of Biological Chemistry, 277 . pp. 25707-25714. ISSN 0021-9258 (doi:10.1074/jbc.M201015200)

Lyall, F., Lye, S., Teoh, T., Cousins, F., Milligan, G., and Robson, S. (2002) Expression of Gs alpha, connexin-43, connexin-26, and EP1, 3, and 4 receptors in myometrium of prelabor singleton versus multiple gestations and the effects of mechanical stretch and steroids on Gs alpha. Journal of the Society For Gynecologic Investigation, 9 . pp. 299-307.

Massotte, D., Brillet, K., Kieffer, B., and Milligan, G. (2002) Agonists activate G(i1)alpha or G(i2)alpha fused to the human mu opioid receptor differently. Journal of Neurochemistry, 81 . pp. 1372-1382.

McLean, A., Zeng, F., Behan, D., Chalmers, D., and Milligan, G. (2002) Generation and analysis of constitutively active and physically destabilized mutants of the human beta(1)-adrenoceptor. Molecular Pharmacology, 62 . pp. 747-755.

Milligan, G. (2002) Construction and analysis of function of G protein-coupled receptor-G protein fusion proteins. G Protein Pathways, Pt A, Receptors, 343 . pp. 260-273.

Milligan, G. (2002) Strategies to identify ligands for orphan G-protein-coupled receptors. Biochemical Society Transactions, 30 . pp. 789-793.

Milligan, G. (2002) The use of receptor G-protein fusion proteins for the study of ligand activity. Receptors and Channels, 8 . pp. 309-317. (doi:10.1080/10606820290005281)

Milligan, G., Stevens, P., Ramsay, D., and McLean, A. (2002) Ligand rescue of constitutively active mutant receptors. Neurosignals, 11 . pp. 29-33.

Ramsay, D., Kellett, E., McVey, M., Rees, S., and Milligan, G. (2002) Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less. Biochemical Journal, 365 . pp. 429-440. ISSN 0264-6021 (doi:10.1042/BJ20020251)

Ward, R., and Milligan, G. (2002) Reciprocal mutations of highly conserved residues in transmembrane helices 2 and 7 of the alpha(2A)-adrenoceptor restore agonist activation of G(i1 alpha). Cellular Signalling, 14 . pp. 139-144.

Welsby, P., Carr, I., Wilkinson, G., and Milligan, G. (2002) Regulation of the avidity of ternary complexes containing the human 5-HT1A receptor by mutation of a receptor contact site on the interacting G protein alpha subunit. British Journal of Pharmacology, 137 . pp. 345-352. (doi:10.1038/sj.bjp.0704880)

Welsby, P., Kellett, E., Wilkinson, G., and Milligan, G. (2002) Enhanced detection of receptor constitutive activity in the presence of regulators of G protein signaling: Applications to the detection and analysis of inverse agonists and low-efficacy partial agonists. Molecular Pharmacology, 61 . pp. 1211-1221.

2001

Milligan, G., and White, J. H. (2001) Protein-protein interactions at G-protein-coupled receptors. Trends in Pharmacological Sciences, 22 (10). pp. 513-518. ISSN 0165-6147 (doi:10.1016/S0165-6147(00)01801-0)

Evans, N., Groarke, D., Warrack, J., Greenwood, C., Dodgson, K., Milligan, G., and Wilson, S. (2001) Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. Journal of Neurochemistry, 77 . pp. 476-485.

Fernandez-Fernandez, J., Abogadie, F., Milligan, G., Delmas, P., and Brown, D. (2001) Multiple pertussis toxin-sensitive G-proteins can couple receptors to GIRK channels in rat sympathetic neurons when expressed heterologously, but only native G(i)-proteins do so in situ. European Journal of Neuroscience, 14 . pp. 283-292.

Groarke, D., Drmota, T., Bahia, D., Evans, N., Wilson, S., and Milligan, G. (2001) Analysis of the C-terminal tail of the rat thyrotropin-releasing hormone receptor-1 in interactions and cointernalization with beta-arrestin 1-green fluorescent protein. Molecular Pharmacology, 59 . pp. 375-385.

Hoffmann, M., Ward, R., Cavalli, A., Carr, I., and Milligan, G. (2001) Differential capacities of the RGS1, RGS16 and RGS-GAIP regulators of G protein signaling to enhance alpha(2A)-adrenoreceptor agonist-stimulated GTPase activity of G(o1)alpha. Journal of Neurochemistry, 78 . pp. 797-806.

McVey, M., Ramsay, D., Kellett, E., Rees, S., Wilson, S., Pope, A., and Milligan, G. (2001) Monitoring receptor oligomerization using time-resolved fluorescence resonance energy transfer and bioluminescence resonance energy transfer - The human delta-opioid receptor displays constitutive oligomerization at the cell surface, which is not regulate. Journal of Biological Chemistry, 276 . pp. 14092-14099. ISSN 0021-9258

Millan, M. et al. (2001) Antiparkinsonian agent piribedil displays antagonist properties at native, rat, and cloned, human alpha(2)-adrenoceptors: Cellular and functional characterization. Journal of Pharmacology and Experimental Therapeutics, 297 . pp. 876-887.

Milligan, G. (2001) Oligomerisation of G-protein-coupled receptors. Journal of Cell Science, 114 . pp. 1265-1271.

Milligan, G. (2001) The human d opioid receptor activates Gi1a more efficiently than Go1a. Journal of Neurochemistry, 76 (6). pp. 1805-1813. (doi:10.1046/j.1471-4159.2001.00196.x)

Milligan, G., Kellett, E., Dacquet, C., Dubreuil, V., Jacoby, E., Millan, M., Lavielle, G., and Spedding, M. (2001) S 14506: novel receptor coupling at 5-HT1A receptors. Neuropharmacology, 40 . pp. 334-344.

Moon, H., Bahia, D., Cavalli, A., Hoffmann, M., and Milligan, G. (2001) Control of the efficiency of agonist-induced information transfer and stability of the ternary complex containing the delta opioid receptor and the alpha subunit of G(i1) by mutation of a receptor/G protein contact interface. Neuropharmacology, 41 . pp. 321-330.

Moon, H., Cavalli, A., Bahia, D., Hoffmann, M., Massotte, D., and Milligan, G. (2001) The human delta opioid receptor activates G(i1)alpha more efficiently than G(o1)alpha. Journal of Neurochemistry, 76 . pp. 1805-1813.

Ramsay, D., Bevan, N., Rees, S., and Milligan, G. (2001) Detection of receptor ligands by monitoring selective stabilization of a Renilla luciferase-tagged, constitutively active mutant, G-protein-coupled receptor. British Journal of Pharmacology, 133 . pp. 315-323.

Stevens, P., Pediani, J., Carrillo, J., and Milligan, G. (2001) Coordinated agonist regulation of receptor and G protein palmitoylation and functional rescue of palmitoylation-deficient mutants of the G protein G(11)alpha following fusion to the alpha(1b)-adrenoreceptor - Palmitoylation of G(11 alpha) is not required. Journal of Biological Chemistry, 276 . pp. 35883-35890. ISSN 0021-9258

This list was generated on Wed Oct 1 14:11:39 2014 BST.
Number of items: 233.

Article

Hudson, B. D., Christiansen, E., Murdoch, H., Jenkins, L., Hansen, A. H., Madsen, O., Ulven, T., and Milligan, G. (2014) Complex pharmacology of novel allosteric free fatty acid 3 receptor ligands. Molecular Pharmacology, 86 (2). pp. 200-210. ISSN 0026-895X (doi:10.1124/mol.114.093294)

Hudson, B. D., Shimpukade, B., Milligan, G., and Ulven, T. (2014) The molecular basis of ligand interaction at free fatty acid receptor 4 (FFA4/GPR120). Journal of Biological Chemistry, 289 . pp. 20345-20358. ISSN 0021-9258 (doi:10.1074/jbc.M114.561449)

Zakrys, L., Ward, R. J., Pediani, J. D., Godin, A. G., Graham, G. J., and Milligan, G. (2014) Roundabout 1 exists predominantly as a basal dimeric complex and this is unaffected by binding of the ligand Slit2. Biochemical Journal, 461 (1). pp. 61-73. ISSN 0264-6021 (doi:10.1042/BJ20140190)

Butcher, A. J., Hudson, B. D., Shimpukade, B., Alvarez-Curto, E., Prihandoko, R., Ulven, T., Milligan, G., and Tobin, A. B. (2014) Concomitant action of structural elements and receptor phosphorylation determine arrestin-3 interaction with the free fatty acid receptor FFA4. Journal of Biological Chemistry, 289 . pp. 18451-18465. ISSN 0021-9258 (doi:10.1074/jbc.M114.568816)

Fjaere, E. et al. (2014) Indomethacin treatment prevents high fat diet-induced obesity and insulin resistance but not glucose intolerance in C57BL/6J mice. Journal of Biological Chemistry, 289 (23). pp. 16032-16045. ISSN 0021-9258 (doi:10.1074/jbc.M113.525220)

McKinney, C. A., Fattah, C., Loughrey, C. M., Milligan, G., and Nicklin, S. A. (2014) Angiotensin-(1-7) and angiotensin-(1-9): function in cardiac and vascular remodeling. Clinical Science, 126 . pp. 815-827. ISSN 0143-5221 (doi:10.1042/CS20130436)

Milligan, G., Ulven, T., Murdoch, H., and Hudson, B. D. (2014) G-protein-coupled receptors for free fatty acids: nutritional and therapeutic targets. British Journal of Nutrition, 111 (S1). S3-S7. ISSN 0007-1145 (doi:10.1017/S0007114513002249)

Ferre, S., Casado, V., Devi, L. A., Filizola, M., Jockers, R., Lohse, M. J., Milligan, G., Pin, J.-P., and Guitart, X. (2014) G protein-coupled receptor oligomerization revisited: functional and pharmacological perspectives. Pharmacological Reviews, 66 (2). pp. 413-434. ISSN 0031-6997 (doi:10.1124/pr.113.008052)

Ward, R.J., and Milligan, G. (2014) Structural and biophysical characterisation of G protein-coupled receptor ligand binding using resonance energy transfer and fluorescent labelling techniques. Biochimica et Biophysica Acta (BBA): Biomembranes, 1838 (1A). pp. 3-14. ISSN 0005-2736 (doi:10.1016/j.bbamem.2013.04.007)

Mackenzie, A.E. et al. (2014) The antiallergic mast cell stabilizers lodoxamide and bufrolin as the first high and equipotent agonists of human and rat gpr35. Molecular Pharmacology, 85 (1). pp. 91-104. ISSN 0026-895X (doi:10.1124/mol.113.089482)

Watterson, K. R., Hudson, B. D., Ulven, T., and Milligan, G. (2014) Treatment of type 2 diabetes by free fatty acid receptor agonists. Frontiers in Endocrinology . ISSN 1664-2392 (doi:10.3389/fendo.2014.00137) (Early Online Publication)

Horobin, R.W., Rashid-Doubell, F., Pediani, J.D., and Milligan, G. (2013) Predicting small molecule fluorescent probe localization in living cells using QSAR modeling. 1. Overview and models for probes of structure, properties and function in single cells. Biotechnic and Histochemistry, 88 (8). pp. 440-460. ISSN 1052-0295 (doi:10.3109/10520295.2013.780634)

Hudson, B.D., Shimpukade, B., Mackenzie, A.E., Butcher, A.J., Pediani, J.D., Christiansen, E., Heathcote, H., Tobin, A.B., Ulven, T., and Milligan, G. (2013) The pharmacology of TUG-891, a potent and selective agonist of the free fatty acid receptor 4 (FFA4/GPR120), demonstrates both potential opportunity and possible challenges to therapeutic agonism. Molecular Pharmacology, 84 (5). pp. 710-725. ISSN 0026-895X (doi:10.1124/mol.113.087783)

Perez-Aso, M., Segura, V., Montó, F., Barettino, D., Noguera, M.A., Milligan, G., and D'Ocon, P. (2013) The three α1-adrenoceptor subtypes show different spatio-temporal mechanisms of internalization and ERK1/2 phosphorylation. Biochimica et Biophysica Acta: Molecular Cell Research, 1833 (10). pp. 2322-2333. ISSN 0167-4889 (doi:10.1016/j.bbamcr.2013.06.013)

Scott, E., Loya, K., Mountford, J., Milligan, G., and Baker, A. H. (2013) MicroRNA regulation of endothelial homeostasis and commitment—implications for vascular regeneration strategies using stem cell therapies. Free Radical Biology and Medicine, 64 . pp. 52-60. ISSN 0891-5849 (doi:10.1016/j.freeradbiomed.2013.04.037)

Hudson, B.D., Murdoch, H., and Milligan, G. (2013) The effects of species ortholog and SNP variation on receptors for free fatty acids. Molecular Endocrinology, 27 (8). pp. 1177-1187. ISSN 0888-8809 (doi:10.1210/me.2013-1085)

Milligan, G. (2013) The prevalence, maintenance, and relevance of G protein-coupled receptor oligomerization. Molecular Pharmacology, 84 (1). pp. 158-169. ISSN 0026-895X (doi:10.1124/mol.113.084780)

Hudson, B.D. et al. (2013) Defining the molecular basis for the first potent and selective orthosteric agonists of the FFA2 free fatty acid receptor. Journal of Biological Chemistry, 288 (24). pp. 17296-17312. ISSN 0021-9258 (doi:10.1074/jbc.M113.455337)

Christiansen, E. et al. (2013) Discovery of TUG-770: a highly potent free fatty acid receptor 1 (FFA1/GPR40) agonist for treatment of type 2 diabetes. ACS Medicinal Chemistry Letters, 4 (5). pp. 441-445. ISSN 1948-5875 (doi:10.1021/ml4000673)

Neetoo-Isseljee, Z., MacKenzie, A.E., Southern, C., Jerman, J., McIver, E. G., Harries, N., Taylor, D. L., and Milligan, G. (2013) High-throughput identification and characterization of novel, species-selective GPR35 agonists. Journal of Pharmacology and Experimental Therapeutics, 344 (3). pp. 568-578. ISSN 1521-0103 (doi:10.1124/jpet.112.201798)

Christiansen, E. et al. (2013) Discovery of a potent and selective free fatty acid receptor 1 agonist with low lipophilicity and high oral bioavailability. Journal of Medicinal Chemistry, 56 (3). pp. 982-992. ISSN 0022-2623 (doi:10.1021/jm301470a)

Clarke, C., Flores-Muñoz, M., McKinney, C. A., Milligan, G., and Nicklin, S. A. (2013) Regulation of cardiovascular remodeling by the counter-regulatory axis of the renin–angiotensin system. Future Cardiology, 9 (1). pp. 23-38. ISSN 1479-6678 (doi:10.2217/fca.12.75)

Howard, L., Mackenzie, R. M., Pchelintsev, N. A., McBryan, T., McClure, J. D., McBride, M. W., Kane, N. M., Adams, P. D., Milligan, G., and Baker, A. H. (2013) Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment. Stem Cell Research and Therapy, 4 (2). Art. 36. ISSN 1757-6512 (doi:10.1186/scrt192)

Hudson, B. D., Ulven, T., and Milligan, G. (2013) The therapeutic potential of allosteric ligands for free fatty acid sensitive GPCRs. Current Topics in Medicinal Chemistry, 13 (1). pp. 14-25. ISSN 1568-0266 (doi:10.2174/1568026611313010004)

Jenkins, L., Harries, N., Lappin, J.E., MacKenzie, A.E., Neetoo-Isseljee, Z., Southern, C., McIver, E.G., Nicklin, S.A., Taylor, D.L., and Milligan, G. (2013) Antagonists of GPR35 display high species ortholog selectivity and varying modes of action. Journal of Pharmacology and Experimental Therapeutics, 343 (3). pp. 683-695. ISSN 0022-3565 (doi:10.1124/jpet.112.198945)

Patowary, S., Alvarez-Curto, E., Xu, T.-R., Holz, J.D., Oliver, J.A., Milligan, G., and Raicu, V. (2013) The muscarinic M3acetylcholine receptor exists as two differently sized complexes at the plasma membrane. Biochemical Journal, 452 (2). pp. 303-312. ISSN 0264-6021 (doi:10.1042/BJ20121902)

Segura, V., Pérez-Aso, M., Montó, F., Carceller, E., Noguera, M.A., Pediani, J., Milligan, G., McGrath, I.C., and D’Ocon, P. (2013) Differences in the signaling pathways of α1A- and α1B-adrenoceptors are related to different endosomal targeting. PLoS ONE, 8 (5). e64996. ISSN 1932-6203 (doi:10.1371/journal.pone.0064996)

Ward, R.W., Xu, T.-X., and Milligan, G. (2013) GPCR oligomerization and receptor trafficking. Methods in Enzymology, 521 . pp. 69-90. ISSN 0076-6879 (doi:10.1016/B978-0-12-391862-8.00004-1)

Moreno, J.L. et al. (2012) Identification of three residues essential for 5-hydroxytryptamine 2A-metabotropic glutamate 2 (5-HT2A{middle dot}mGlu2) receptor heteromerization and its psychoactive behavioral function. Journal of Biological Chemistry, 287 (53). pp. 44301-44319. ISSN 0021-9258 (doi:10.1074/jbc.M112.413161)

Hudson, B., Christiansen, E., Tikhonova, I.G., Grundmann, M., Kostenis, E., Adams, D.R., Ulven, T., and Milligan, G. (2012) Chemically engineering ligand selectivity at the free fatty acid receptor 2 based on pharmacological variation between species orthologs. FASEB Journal, 26 (12). pp. 4951-4965. ISSN 0892-6638 (doi:10.1096/fj.12-213314 )

Hudson, B.D., Tikhonova, I.G., Pandey, S.K., Ulven, T., and Milligan, G. (2012) Extracellular ionic locks determine variation in constitutive activity and ligand potency between species orthologs of the free fatty acid receptors FFA2 and FFA3. Journal of Biological Chemistry, 287 (49). pp. 41195-41209. ISSN 0021-9258 (doi:10.1074/jbc.M112.396259)

Christiansen, E. et al. (2012) Free fatty acid receptor 1 (FFA1/GPR40) agonists: mesylpropoxy appendage lowers lipophilicity and improves ADME properties. Journal of Medicinal Chemistry, 55 (14). pp. 6624-6628. ISSN 0022-2623 (doi:10.1021/jm3002026)

Cunningham, M. R., McIntosh, K. A., Pediani, J. D., Robben, J., Cooke, A. E., Nilsson, M., Gould, G. W., Mundell, S., Milligan, G., and Plevin, R. (2012) Novel role for proteinase-activated receptor 2 (PAR2) in membrane trafficking of proteinase-activated receptor 4 (PAR4). Journal of Biological Chemistry, 287 (20). pp. 16656-16669. ISSN 0021-9258 (doi:10.1074/jbc.M111.315911)

Shimpukade, B., Hudson, B.D., Hogaard, C.K., Milligan, G., and Ulven, T. (2012) Discovery of a potent and selective GPR120 agonist. Journal of Medicinal Chemistry, 55 (9). pp. 4511-4515. ISSN 0022-2623 (doi:10.1021/jm300215x)

Xu, T.-R., Ward, R. J., Pediani, J. D., and Milligan, G. (2012) Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation. Journal of Biological Chemistry, 287 . pp. 14937-14949. ISSN 0021-9258 (doi:10.1074/jbc.M111.334300)

Kane, N. M. et al. (2012) Role of MicroRNAs 99b, 181a, and 181b in the differentiation of human embryonic stem cells to vascular endothelial cells. Stem Cells, 30 (4). pp. 643-654. ISSN 1066-5099 (doi:10.1002/stem.1026)

White, K., Kane, N. M., Milligan, G., and Baker, A. H. (2012) The role of miRNA in stem cell pluripotency and commitment to the vascular endothelial lineage. Microcirculation, 19 (3). pp. 196-207. ISSN 1073-9688 (doi:10.1111/j.1549-8719.2012.00161.x)

Xu, T.-R., Lu, R.-F., Romano, D., Pitt, A., Houslay, M. D., Milligan, G., and Kolch, W. (2012) Eukaryotic translation initiation factor 3, subunit a, regulates the extracellular signal-regulated kinase pathway. Molecular and Cellular Biology, 32 (1). pp. 88-95. ISSN 0270-7306 (doi:10.1128/​MCB.05770-11)

Flores, M., Graham, D., Dominiczak, A.F., Milligan, G., Baker, A.H., and Nicklin, S.A. (2012) Angiotensin-(1-9) antagonises Angiotensin II-induced cardiac remodeling via the angiotensin type 2 receptor. Proceedings of the Physiological Society, 27 . C1. ISSN 1749-6187

Flores, M., Graham, D., Dominiczak, A. F., Milligan, G., Baker, A. H., and Nicklin, S. A. (2012) Abstract 231: angiotensin-(1-9) antagonises cardiac remodelling in a mouse model of Angiotensin Ii-induced hypertension. Hypertension, 60 . A231. ISSN 0194-911X

Georgoussi, Z., Georganta, E.M., and Milligan, G. (2012) The other side of opioid receptor signalling: regulation by protein-protein interaction. Current Drug Targets, 13 (1). pp. 80-102. ISSN 1389-4501 (doi:10.2174/138945012798868470)

Laarman, A.J. et al. (2012) Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO Journal, 31 (17). pp. 3607-3619. ISSN 0261-4189 (doi:10.1038/emboj.2012.212)

Pou, C., Mannoury la Cour, C., Stoddart, L.A., Millan, M.J., and Milligan, G. (2012) Functional homomers and heteromers of dopamine D2L and D3 receptors co-exist at the cell surface. Journal of Biological Chemistry, 287 (12). pp. 8864-8878. ISSN 0021-9258 (doi:10.1074/jbc.M111.326678)

Terhzaz, S., Cabrero, P., Robben, J.H., Radford, J.C., Hudson, B.D., Milligan, G., Dow, J.A.T., and Davies, S.A. (2012) Mechanism and function of drosophila capa GPCR: a desiccation stress-responsive receptor with functional homology to human neuromedinU receptor. PLoS ONE, 7 (1). e29897. ISSN 1932-6203 (doi:10.1371/journal.pone.0029897)

Alvarez-Curto, E., Prihandoko, R., Tautermann, C. S. , Zwier, J. M., Pediani, J. D., Lohse, M. J., Hoffmann, C., Tobin, A. B., and Milligan, G. (2011) Developing chemical genetic approaches to explore G protein-coupled receptor function: validation of the use of a Receptor Activated Solely by Synthetic Ligand (RASSL). Molecular Pharmacology, 80 (6). pp. 1033-1046. ISSN 0026-895X (doi:10.1124/mol.111.074674)

Ward, R. J., Pediani, J. D., and Milligan, G. (2011) Heteromultimerization of cannabinoid CB1 receptor and orexin OX1 receptor generates a unique complex in which both protomers are regulated by orexin A. Journal of Biological Chemistry, 286 (43). pp. 37414-37428. ISSN 0021-9258 (doi:10.1074/jbc.M111.287649)

Howard, L., Kane, N., Milligan, G., and Baker, A. H. (2011) MicroRNAs regulating cell pluripotency and vascular differentiation. Vascular Pharmacology, 55 (4). pp. 69-78. ISSN 1537-1891 (doi:10.1016/j.vph.2011.08.002)

Xu, T.‑R. , Ward, R. J., Pediani, J. D., and Milligan, G. (2011) The orexin OX1 receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands. Biochemical Journal, 439 (1). pp. 171-183. ISSN 0264-6021 (doi:10.1042/BJ20110230)

Van Lith, M., Tiwari, S., Pediani, J., Milligan, G., and Bulleid, N. J. (2011) Real-time monitoring of redox changes in the mammalian endoplasmic reticulum. Journal of Cell Science, 124 (14). pp. 2349-2356. ISSN 0021-9533 (doi:10.1242/jcs.085530)

Smith, N.J. et al. (2011) Extracellular loop 2 of the free fatty acid receptor 2 mediates allosterism of a phenylacetamide ago-allosteric modulator. Molecular Pharmacology, 80 (1). pp. 163-173. ISSN 0026-895X (doi:10.1124/mol.110.070789)

Schmidt, J. et al. (2011) Selective orthosteric free fatty acid receptor 2 (FFA2) agonists: identification of the structural and chemical requirements for selective activation of FFA2 versus FFA3. Journal of Biological Chemistry, 286 (12). pp. 10628-10640. ISSN 0021-9258 (doi:10.1074/jbc.M110.210872)

Anthony, D.F., Sin, Y.Y., Vadrevu, S., Advant, N., Day, J.P., Byrne, A.M., Lynch, M.J., Milligan, G., Houslay, M.D., and Baillie, G.S. (2011) β Arrestin 1 inhibits the GAP function of ARHGAP21 so as to promote the activation of RhoA following angiotensin II type 1A receptor stimulation. Molecular and Cellular Biology, 31 (5). pp. 1066-1075. ISSN 0270-7306 (doi:10.1128/MCB.00883-10)

Ward, R. J., Pediani, J. D., and Milligan, G. (2011) Ligand-induced internalisation of the orexin OX1 and cannabinoid CB1 receptors assessed via N-terminal SNAP and CLIP-tagging. British Journal of Pharmacology, 162 (6). pp. 1439-1452. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2010.01156.x)

Ward, R. J., Pediani, J. D., and Milligan, G. (2011) Ligand-induced internalization of the orexin OX1 and cannabinoid CB1 receptors assessed via N-terminal SNAP and CLIP-tagging. British Journal of Pharmacology, 162 (6). pp. 1439-1452. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2010.01156.x)

Flores-Munoz, M., Smith, N. J., Haggerty, C., Milligan, G.,, and Nicklin, S. A. (2011) Angiotensin1-9 antagonises pro-hypertrophic signalling in cardiomyocytes via the angiotensin type 2 receptor. Journal of Physiology, 589 (4). pp. 939-951. ISSN 0022-3751 (doi:10.1113/jphysiol.2010.203075)

Smith, N.J., Bennett, K., and Milligan, G. (2011) When simple agonism is not enough: Emerging modalities of GPCR ligands. Molecular and Cellular Endocrinology, 331 (2). pp. 241-247. ISSN 0303-7207 (doi:10.1016/j.mce.2010.07.009)

Dimond, P. et al. (2011) G protein-coupled receptor modulation with pepducins: moving closer to the clinic. Annals of the New York Academy of Sciences, 1226 (1). pp. 34-49. ISSN 0077-8923 (doi:10.1111/j.1749-6632.2011.06039.x)

Milligan, G. (2011) Orthologue selectivity and ligand bias: translating the pharmacology of GPR35. Trends in Pharmacological Sciences, 32 (5). pp. 317-325. ISSN 0165-6147 (doi:10.1016/j.tips.2011.02.002)

Nejsum, L.N., Christensen, T.M., Robben, J.H., Milligan, G., Deen, P.M.T., Bichet, D.G., and Levin, K. (2011) Novel mutation in the AVPR2 gene in a Danish male with nephrogenic diabetes insipidus caused by ER retention and subsequent lysosomal degradation of the mutant receptor. NDT Plus, 4 (3). pp. 158-163. ISSN 1753-0784 (doi:10.1093/ndtplus/sfr010)

Schröder, R. et al. (2011) Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells. Nature Protocols, 6 (11). pp. 1748-1760. ISSN 1754-2189 (doi:10.1038/nprot.2011.386)

Tarrasón, G. et al. (2011) The sphingosine-1-phosphate receptor-1 antagonist, W146, causes early and short-lasting peripheral blood lymphopenia in mice. International Immunopharmacology, 11 (11). pp. 1773-1779. ISSN 1567-5769 (doi:10.1016/j.intimp.2011.07.004)

Ward, R.J., Alvarez-Curto, E., and Milligan, G. (2011) Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression. Methods in Molecular Biology, 746 . pp. 21-37. ISSN 1064-3745 (doi:10.1007/978-1-61779-126-0_2)

Burton, P. et al. (2010) Erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) blocks differentiation and maintains the expression of pluripotency markers in human embryonic stem cells. Biochemical Journal, 432 (3). pp. 575-584. ISSN 0264-6021 (doi:10.1042/BJ20100726)

Kane, N. M. et al. (2010) Lentivirus-mediated reprogramming of somatic cells in the absence of transgenic transcription factors. Molecular Therapy, 18 (12). pp. 2139-2145. ISSN 1525-0016 (doi:10.1038/mt.2010.231)

Smith, N. J., and Milligan, G. (2010) Allostery at G protein-coupled receptor Homo- and Heteromers: uncharted pharmacological landscapes. Pharmacological Reviews, 62 (4). pp. 701-725. ISSN 0031-6997 (doi:10.1124/pr.110.002667)

Lopez-Gimenez, J.F., and Milligan, G. (2010) Opioid regulation of Mu receptor internalisation: relevance to the development of tolerance and dependence. CNS and Neurological Disorders: Drug Targets, 9 (5). pp. 616-626. ISSN 1871-5273 (doi:10.2174/187152710793361522)

Alvarez-Curto, E., Pediani, J. D., and Milligan, G. (2010) Applications of fluorescence and bioluminescence resonance energy transfer to drug discovery at G protein coupled receptors. Analytical and Bioanalytical Chemistry, 398 (1). pp. 167-180. ISSN 1618-2642 (doi:10.1007/s00216-010-3823-4)

Schroder, R. et al. (2010) Deconvolution of complex G protein-coupled receptor signaling in live cells using dynamic mass redistribution measurements. Nature Biotechnology, 28 (9). pp. 943-949. ISSN 1087-0156 (doi:10.1038/nbt.1671)

Burton, P. et al. (2010) Identification and characterization of small-molecule ligands that maintain pluripotency of human embryonic stem cells. Biochemical Society Transactions, 38 (4). pp. 1058-1061. ISSN 0300-5127 (doi:10.1042/BST0381058)

Alvarez-Curto, E., Ward, R. J., Pediani, J. D., and Milligan, G. (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry, 285 (30). pp. 23318-23330. ISSN 0021-9258 (doi:10.1074/jbc.M110.122184)

Khelashvili, G. et al. (2010) GPCR-OKB: the G protein coupled receptor oligomer knowledge base. Bioinformatics, 26 (14). pp. 1804-1805. ISSN 1367-4803 (doi:10.1093/bioinformatics/btq264)

Kane, N. M., Meloni, M. , Spencer, H. L., Craig, M. A. , Strehl, R., Milligan, G., Houslay, M. D., Mountford, J. C., Emanueli, C., and Baker, A. H. (2010) Derivation of endothelial cells from human embryonic stem cells by directed differentiation: analysis of microRNA and angiogenesis in vitro and in vivo. Arteriosclerosis, Thrombosis and Vascular Biology, 30 (7). pp. 1389-1397. ISSN 1079-5642 (doi:10.1161/ATVBAHA.110.204800)

del Burgo, L. S., and Milligan, G. (2010) Heterodimerisation of G protein-coupled receptors: implications for drug design and ligand screening. Expert Opinion on Drug Discovery, 5 (5). pp. 461-474. ISSN 1746-0441 (doi:10.1517/17460441003720467)

Milligan, G. (2010) The role of dimerisation in the cellular trafficking of G-protein-coupled receptors. Current Opinion in Pharmacology, 10 (1). pp. 23-29. ISSN 1471-4892 (doi:10.1016/j.coph.2009.09.010)

Andrews, P. et al. (2010) High content screening of feeder-free human embryonic stem cells to identify pro-survival small molecules. Biochemical Journal, 432 (Pt 1). pp. 21-33. ISSN 0264-6021 (doi:10.1042/BJ20101022)

Burton, P. et al. (2010) Identification and characterization of small-molecule ligands that maintain pluripotency of human embryonic stem cells. Biochemical Society Transactions, 38 (4). pp. 1058-1061. ISSN 0300-5127 (doi:10.1042/BST0381058)

Jenkins, L., Alvarez-Curto, E., Campbell, K., De Munnik, S., Canals Buj, M., Schlyer, S., and Milligan, G. (2010) Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Gα13 and β-arrestin-2. British Journal of Pharmacology, 162 (3). pp. 733-748. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2010.01082.x)

Jenkins, L., Brea, J., Smith, N.J., Hudson, B.D., Reilly, G., Bryant, N.J., Castro, M., Loza, M.I., and Milligan, G. (2010) Identification of novel species-selective agonists of the G-protein-coupled receptor GPR35 that promote recruitment of β-arrestin-2 and activate Gα13. Biochemical Journal, 432 (3). pp. 451-459. ISSN 0264-6021 (doi:10.1042/BJ20101287)

Stoddart, L.A., and Milligan, G. (2010) Constitutive activity of GPR40/FFA1 intrinsic or assay dependent? Methods in Enzymology, 484 . pp. 569-590. ISSN 0076-6879 (doi:10.1016/B978-0-12-381298-8.00028-9)

Xu, T. et al. (2010) Inferring signaling pathway topologies from multiple perturbation measurements of specific biochemical species. Science Signaling, 3 (113). ra20. ISSN 1945-0877 (doi:10.1126/scisignal.2000517)

Robben, J.H., Fenton, R.A., Vargas, S.L., Schweer, H., Peti-Peterdi, J., Deen, P.M.T., and Milligan, G. (2009) Localization of the succinate receptor in the distal nephron and its signaling in polarized MDCK cells. Kidney International, 76 (12). pp. 1258-1267. ISSN 0085-2538 (doi:10.1038/ki.2009.360)

Milligan, G. (2009) G protein-coupled receptor hetero-dimerization: contribution to pharmacology and function. British Journal of Pharmacology, 158 (1). pp. 5-14. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2009.00169.x)

Milligan, G., and McGrath, J.C. (2009) GPCR theme editorial. British Journal of Pharmacology, 158 (1). pp. 1-4. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2009.00422.x)

Robben, J.H., Kortenoeven, M.L.A., Sze, M., Yae, C., Milligan, G., Oorschot, V.M., Klumperman, J., Knoers, N.V.A.M., and Deen, P.M.T. (2009) Intracellular activation of vasopressin V2 receptor mutants in nephrogenic diabetes insipidus by nonpeptide agonists. Proceedings of the National Academy of Sciences of the United States of America, 106 (29). pp. 12195-12200. ISSN 0027-8424 (doi:10.1073/pnas.0900130106)

Smith, N.J., Stoddart, L.A., Devine, N.M., Jenkins, L., and Milligan, G. (2009) The action and mode of binding of thiazolidinedione ligands at free fatty acid receptor 1. Journal of Biological Chemistry, 284 (26). pp. 17527-17539. ISSN 0021-9258 (doi:10.1074/jbc.M109.012849)

Idris, A.I., Sophocleous, A., Landao-Bassonga, E., Merkouris, M., Milligan, G., Baker, D., van't Hof, R.J., and Ralston, S.H. (2009) Cannabinoid receptor type 1 protects against age-related bone loss by regulating osteoblast and adipocyte differentiation of bone marrow stromal cells. Bone, 44 (Supple). S222. ISSN 8756-3282 (doi:10.1016/j.bone.2009.03.067)

Ward, R. J., Jenkins, L., and Milligan, G. (2009) Selectivity and functional consequences of interactions of family A G protein-coupled receptors with neurochondrin and periplakin. Journal of Neurochemistry, 109 (1). pp. 182-192. ISSN 0022-3042 (doi:10.1111/j.1471-4159.2009.05918.x)

Ferre, S. et al. (2009) Building a new conceptual framework for receptor heteromers. Nature Chemical Biology, 5 (3). pp. 131-134. ISSN 1552-4450 (doi:10.1038/nchembio0309-131)

Canals, M., Lopez-Gimenez, J. F. , and Milligan, G. (2009) Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective α1b-adrenoceptor mutant demonstrates membrane targeting of GPCR oligomers. Biochemical Journal, 417 (1). pp. 161-172. ISSN 0264-6021 (doi:10.1042/BJ20081227)

Bennett, K.A., Langmead, C.J., Wise, A., and Milligan, G. (2009) Growth hormone secretagogues and growth hormone releasing peptides act as orthosteric super-agonists but not allosteric regulators for activation of the G protein G{alpha}o1 by the ghrelin receptor. Molecular Pharmacology, 76 (4). pp. 802-811. ISSN 0026-895X (doi:10.1124/mol.109.056101)

Brea, J.H. et al. (2009) Evidence for distinct antagonist-revealed functional states of 5-HT2A receptor homodimers. Molecular Pharmacology, 75 (6). pp. 1380-1391. ISSN 0026-895X (doi:10.1124/mol.108.054395)

Idris, A. I., Sophocleous, A., Landa-Bassonga, E., Canals, M., Milligan, G., Baker, D., van't Hof, R. J., and Ralston, S. (2009) Cannabinoid receptor type 1 protects against age-related bone loss by regulating osteoblast and adipocyte differentiation of bone marrow stromal cells. Cell Metabolism, 10 (2). pp. 139-147. ISSN 1550-4131 (doi:10.1016/j.cmet.2009.07.006)

Milligan, G., Stoddart, L.A., and Smith, N.J. (2009) Agonism and allosterism: the pharmacology of the free fatty acid receptors FFA2 and FFA3. British Journal of Pharmacology, 158 (1). pp. 146-153. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2009.00421.x)

Lopez-Gimenez, J. F., Milligan, G., and Vilaro, M. T. (2008) Morphine desensitization, internalization, and down-regulation of the μ opioid receptor is facilitated by serotonin 5-hydroxytryptamine2A receptor coactivation. Molecular Pharmacology, 74 (5). pp. 1278-1291. ISSN 0026-895X (doi:10.1124/mol.108.048272)

Lopez-Gimenez, J. F., Vilaro, M. T., and Milligan, G. (2008) Morphine desensitization, internalization and down-regulation of the mu opioid receptor is facilitated by serotonin 5-HT2A receptor co-activation. Molecular Pharmacology, 74 (5). pp. 1278-1291. ISSN 0026-895X (doi:10.1124/mol.108.048272)

Xu, T.-R., Baillie, G. S., Bhari, N., Houslay, T. M., Pitt, A. M., Adams, D. R., Kolch, W., Houslay, M. D., and Milligan, G. (2008) Mutations of β-arrestin 2 that limit self-association also interfere with interactions with the β2-adrenoceptor and the ERK1/2 MAPKs: implications for β2-adrenoceptor signalling via the ERK1/2 MAPKs. Biochemical Journal, 413 (1). pp. 51-60. ISSN 0264-6021 (doi:10.1042/BJ20080685)

Lane, J.R., Henderson, D., Powney, B., Wise, A., Rees, S., Daniels, D., Plumpton, C., Kinghorn, I., and Milligan, G. (2008) Antibodies that identify only the active conformation of Gi family G protein (alpha) subunits. FASEB Journal, 22 (6). pp. 1924-1932. ISSN 0892-6638 (doi:10.1096/fj.07-100388)

Canals Buj, M., and Milligan, G. (2008) Constitutive activity of the cannabinoid CB1 receptor regulates the function of co-expressed Mu opioid receptors. Journal of Biological Chemistry, 283 (17). pp. 11424-11434. ISSN 0021-9258 (doi:10.1074/jbc.M710300200)

González-Maeso, J. et al. (2008) Identification of a serotonin/glutamate receptor complex implicated in psychosis. Nature, 452 (7183). pp. 93-97. ISSN 0028-0836 (doi:10.1038/nature06612)

Milligan, G. (2008) A day in the life of a G protein-coupled receptor: the contribution to function of G protein-coupled receptor dimerization. British Journal of Pharmacology, 153 (.). S216-S229. ISSN 0007-1188 (doi:10.1038/sj.bjp.0707490)

Hill, C., Brownlie, Z., Davey, J., Milligan, G., and Ladds, G. (2008) Isolation and characterization of a novel human RGS mutant displaying gain-of-function activity. Cellular Signalling, 20 (2). pp. 323-336. ISSN 0898-6568 (doi:10.1016/j.cellsig.2007.10.016)

Lane, J.R., Powney, B., Wise, A., Rees, S., and Milligan, G. (2008) G protein-coupling and ligand selectivity of the D2L and D3 dopamine receptors. Journal of Pharmacology and Experimental Therapeutics, 325 (1). pp. 319-330. ISSN 0022-3565 (doi:10.1124/jpet.107.134296)

Lane, J.R., Henderson, D., Powney, B., Wise, A., Rees, S., Daniels, D., Plumpton, C., Kinghorn, I., and Milligan, G. (2008) Antibodies that identify only the active conformation of Gi family G protein α subunits. FASEB Journal, 22 . pp. 1924-1932. ISSN 0892-6638 (doi:10.1096/fj.07-100388)

Lane, J.R., Powney, B., Wise, A., Rees, S., and Milligan, G. (2008) G protein-coupling and ligand selectivity of the D2L and D3 dopamine receptors. Journal of Pharmacology and Experimental Therapeutics, 325 (1). pp. 319-330. ISSN 0022-3565 (doi:10.1124/jpet.107.134296)

McCulloch, C., Morrow, V., Milasta, S., Comerford, I., Milligan, G., Graham, G., Isaacs, N., and Nibbs, R. (2008) Multiple roles for the C-terminal tail of the chemokine scavenger D6. Journal of Biological Chemistry, 283 (12). pp. 7972-7982. ISSN 0021-9258 (doi:10.1074/jbc.M710128200 )

Noubade, R., Saligrama, N., Spach, K., del Rio, R., Blankenhorn, E.P., Kantidakis, T., Milligan, G., Rincon, M., and Teuscher, C. (2008) Autoimmune disease-associated histamine receptor H1 alleles exhibit differential protein trafficking and cell surface expression. Journal of Immunology, 180 (11). pp. 7471-7479. ISSN 0022-1767

Parenty, G., Applebe, S., and Milligan, G. (2008) CXCR2 chemokine receptor antagonism enhances DOP opioid receptor function via allosteric regulation of the CXCR2-DOP receptor hetero-dimer. Biochemical Journal, 412 (Pt2). pp. 215-256. ISSN 0264-6021 (doi:10.1042/BJ20071689)

Snook, L. A., Milligan, G., Kieffer, B. L., and Massotte, D. (2008) Co-expression of mu and delta opioid receptors as receptor-G protein fusions enhances both mu and delta signalling via distinct mechanisms. Journal of Neurochemistry, 105 (3). pp. 865-873. ISSN 0022-3042 (doi:10.1111/j.1471-4159.2008.05215.x)

Stoddart, L. A., Smith, N. J., Jenkins, L., Brown, A. J., and Milligan, G. (2008) Conserved Polar Residues in Transmembrane Domains V, VI, and VII of Free Fatty Acid Receptor 2 and Free Fatty Acid Receptor 3 Are Required for the Binding and Function of Short Chain Fatty Acids. Journal of Biological Chemistry, 283 (47). pp. 32913-32924. ISSN 0021-9258 (doi:10.1074/jbc.M805601200)

Stoddart, L. A., Smith, N. J., and Milligan, G. (2008) International Union of Pharmacology. LXXI. Free Fatty Acid Receptors FFA1, -2, and -3: Pharmacology and Pathophysiological Functions. Pharmacological Reviews, 60 (4). pp. 405-417. ISSN 0031-6997 (doi:10.1124/pr.108.00802)

Snook, L.A., Milligan, G., Kieffer, B.L., and Massotte, D. (2007) Co-expression of mu and delta opioid receptors as receptor-G protein fusions enhances both mu and delta signalling via distinct mechanisms. Journal of Neurochemistry, 105 (3). pp. 865-873. ISSN 0022-3042 (doi:10.1111/j.1471-4159.2008.05215.x)

Noubade, R., Milligan, G., Zachary, J.F., Blankenhorn, E.P., del Rio, R., Rincorn, M., and Teuscher, C. (2007) Histamine receptor H1 is required for TCR-mediated p38 MAPK activation and optimal IFN-γ production in mice. Journal of Clinical Investigation, 117 (11). pp. 3507-3518. ISSN 0021-9738

Baillie, G.S. et al. (2007) Mapping binding sites for the PDE4D5 cAMP-specific phosphodiesterase to the N- and C-domains of beta-arrestin using spot-immobilized peptide arrays. Biochemical Journal, 404 (1). pp. 71-80. ISSN 0264-6021 (doi:10.1042%2FBJ20070005)

Skrabanek, L. et al. (2007) Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base. BMC Bioinformatics, 8 (177). ISSN 1471-2105 (doi:10.1186/1471-2105-8-177)

Lopez-Gimenez, J. F., Canals Buj, M. , Pediani, J. D., and Milligan, G. (2007) The 1b-adrenoceptor exists as a higher-order oligomer: effective oligomerization is required for receptor maturation, surface delivery, and function. Molecular Pharmacology, 71 (4). pp. 1015-1029. ISSN 0026-895X (doi:10.1124/mol.106.033035)

Milligan, G. (2007) G protein-coupled receptor dimerisation: molecular basis and relevance to function. Biochimica et Biophysica Acta: Biomembranes, 1768 (4). pp. 825-835. ISSN 0005-2736 (doi:10.1016/j.bbamem.2006.09.021)

Bouvier, M., Heveker, N., Jockers, R., Marullo, S., and Milligan, G. (2007) BRET analysis of GPCR oligomerization: newer does not mean better. Nature Methods, 4 (1). pp. 3-4. ISSN 1548-7091 (doi:10.1038/nmeth0107-3)

Fredholm, B., Hokfelt, T., and Milligan, G. (2007) G-protein-coupled receptors: an update. Acta Physiologica, 190 . pp. 3-7. (doi:10.1111/j.1748-1716.2007.01689.x)

Jameson, E., Pei, J., Wade, S., Neubig, R., Milligan, G., and Kennedy, R. (2007) Capillary electrophoresis assay for G protein-coupled receptor-mediated GTPase activity. Analytical Chemistry, 79 . pp. 1158-1163. (doi:10.1021/ac061099g)

Lane, J., Powney, B., Wise, A., Rees, S., and Milligan, G. (2007) Protean agonism at the dopamine D-2 receptor: (S)-3-(3-hydroxyphenyl)-N-propylpiperidine is an agonist for activation of G(o1) but an antagonist/inverse agonist for G(i1), G(i2), and G(i3). Molecular Pharmacology, 71 . pp. 1349-1359. (doi:10.1124/mol.106.032722)

Milligan, G. (2007) Allosteric modulation of heterodimeric G-protein-coupled receptors. Trends in Pharmacological Sciences, 28 (12). pp. 615-620. ISSN 0165-6147 (doi:10.1016/j.tips.2007.11.001)

Milligan, G., Parenty, G., Stoddart, L., and Lane, J. (2007) Novel pharmacological applications of G-protein-coupled receptor-G protein fusions. Current Opinion in Pharmacology, 7 . pp. 521-526. (doi:10.1016/j.coph.2007.06.007)

Noubade, R., Milligan, G., Zachary, J., Blankenhorn, E., del Rio, R., Rincon, M., and Teuscherl, C. (2007) Histamine receptor H-1 is-required for TCR-mediated p38 MAPK activation and optimal IFN-gamma production in mice. Journal of Clinical Investigation, 117 . pp. 3507-3518. (doi:10.1172/JC132792)

Orth, J., Lang, S., Preuss, I., Milligan, G., and Aktories, K. (2007) Action of Pasteurella multocida toxin on G alpha(q) is persistent and independent of interaction with G-protein-coupled receptors. Cellular Signalling, 19 . pp. 2174-2182. (doi:10.1016/j.cellsig.2007.06.016)

Pin, J. et al. (2007) International union of basic and clinical pharmacology. LXVII. Recommendations for the recognition and nomenclature of g protein-coupled receptor heteromultimers. Pharmacological Reviews, 59 . pp. 5-13. (doi:10.1124/pr.59.1.5)

Sartania, N., Appelbe, S., Pediani, J. D., and Milligan, G. (2007) Agonist occupancy of a single monomeric element is sufficient to cause internalization of the dimeric beta(2)-adrenoceptor. Cellular Signalling, 19 . pp. 1928-1938. ISSN 0898-6568 (doi:10.1016/j.cellsig.2007.05.002)

Stoddart, L., Brown, A., and Milligan, G. (2007) Uncovering the pharmacology of the G protein-coupled receptor GPR40: High apparent constitutive activity in guanosine 5'-O-(3-[S-35] thio) triphosphate binding studies reflects binding of an endogenous agonist. Molecular Pharmacology, 71 . pp. 994-1005. (doi:10.1124/mol.106.031534)

Ellis, J., Pediani, J.D., Canals Buj, M., Milasta, S., and Milligan, G. (2006) Orexin-1 receptor-cannabinoid CB1 receptor heterodimerization results in both ligand-dependent and -independent coordinated alterations of receptor localization and function. Journal of Biological Chemistry, 281 (25). pp. 38812-38824. ISSN 0021-9258 (doi:10.1074/jbc.M602494200)

Comerford, I., Milasta, S., Morrow, V., Milligan, G., and Nibbs, R. (2006) The chemokine receptor CCX-CKR mediates effective scavenging of CCL19 in vitro. European Journal of Immunology, 36 (7). pp. 1904-1916. ISSN 0014-2980 (doi:10.1002/eji.200535716)

Batenburg, W. et al. (2006) Carvedilol-induced antagonism of angiotensin II: a matter of alpha(1)-adrenoceptor blockade. Journal of Hypertension, 24 . pp. 1355-1363.

Canals, M., Jenkins, L., Kellett, E., and Milligan, G. (2006) Up-regulation of the angiotensin II type 1 receptor by the MAS proto-oncogene is due to constitutive activation of G(q)/G(11) by MAS. Journal of Biological Chemistry, 281 . pp. 16757-16767. ISSN 0021-9258 (doi:10.1074/jbc.M601121200)

Ciruela, F. et al. (2006) Presynaptic control of striatal glutamatergic neurotransmission by adenosine A(1)-A(2A) receptor heteromers. Journal of Neuroscience, 26 . pp. 2080-2087. (doi:10.1523/JNEUROSCI.3574-05.2006)

Francke, F., Ward, R., Jenkins, L., Kellett, E., Richter, D., Milligan, G., and Bachner, D. (2006) Interaction of neurochondrin with the melanin-concentrating hormone receptor 1 interferes with G protein-coupled signal transduction but not agonist-mediated internalization. Journal of Biological Chemistry, 281 . pp. 32496-32507. ISSN 0021-9258 (doi:10.1074/jbc.M602889200)

Milasta, S., Pediani, J., Appelbe, S., Trim, S., Wyatt, M., Cox, P., Fidock, M., and Milligan, G. (2006) Interactions between the Mas-related receptors MrgD and MrgE alter signalling and trafficking of MrgD. Molecular Pharmacology, 69 . pp. 479-491. (doi:10.1124/mol.105.018788)

Milligan, G. (2006) G-protein-coupled receptor heterodimers: pharmacology, function and relevance to drug discovery. Drug Discovery Today, 11 . pp. 541-549. (doi:10.1016/j.drudis.2006.04.007)

Milligan, G., and Kostenis, E. (2006) Heterotrimeric G-proteins: a short history. British Journal of Pharmacology, 147 . S46-S55.

Milligan, G., Pediani, J., Canals, M., and Lopez-Gimenez, J. (2006) Oligomeric structure of the alpha(1b)-adrenoceptor: Comparisons with rhodopsin. Vision Research, 46 . pp. 4434-4441. ISSN 0042-6989 (doi:10.1016/j.visres.2006.08.007)

Milligan, G., Stoddart, L., and Brown, A. (2006) G protein-coupled receptors for free fatty acids. Cellular Signalling, 18 . pp. 1360-1365. (doi:10.1016/j.cellsig.2006.03.011)

Novotny, J., Durchankova, D., Ward, R., Carrillo, J., Svoboda, P., and Milligan, G. (2006) Functional interactions between the alpha(1b)-adrenoceptor and G alpha(11) are compromised by de-palmitoylation of the G protein but not of the receptor. Cellular Signalling, 18 . pp. 1244-1251. (doi:10.1016/j.cellsig.2005.10.004)

Snook, L., Milligan, G., Kieffer, B., and Massotte, D. (2006) mu-delta opioid receptor functional interaction: Insight using receptor-G protein fusions. Journal of Pharmacology and Experimental Therapeutics, 318 . pp. 683-690. (doi:10.1124/jpet.106.101220)

Milligan, G., and Bouvier, M. (2005) Methods to monitor the quaternary structure of G protein-coupled receptors. FEBS Journal, 272 (12). pp. 2914-2925. ISSN 1742-464X (doi:10.1111/j.1742-4658.2005.04731.x)

Barclay, E., OReilly, M., and Milligan, G. (2005) Activation of an alpha(2A)-adrenoceptor-G alpha(o1), fusion protein dynamically regulates the palmitoylation status of the G protein but not of the receptor. Biochemical Journal, 385 . pp. 197-206. ISSN 0264-6021

Barnes, W., Reiter, E., Violin, J., Ren, X., Milligan, G., and Lefkowitz, R. (2005) beta-arrestin 1 and G(alpha q/11) coordinately activate RhoA and stress fiber formation following receptor stimulation. Journal of Biological Chemistry, 280 . pp. 8041-8050. ISSN 0021-9258 (doi:10.1074/jbc.M412924200)

Holdsworth, G., Slocombe, P., Hutchinson, G., and Milligan, G. (2005) Analysis of endogenous S1P and LPA receptor expression in CHO-K1 cells. Gene, 350 . pp. 59-63. (doi:10.1016/j.gene.2005.01.016)

Hunton, D., Barnes, W., Kim, J., Ren, X., Violin, J., Reiter, E., Milligan, G., Patel, D., and Lefkowitz, R. (2005) beta-arrestin 2-dependent angiotensin II type 1A receptor-mediated pathway of chemotaxis. Molecular Pharmacology, 67 . pp. 1229-1236. (doi:10.1124/mol.104.006270)

Kostenis, E., Martini, L., Ellis, J., Waldhoer, M., Heydorn, A., Rosenkilde, M., Norregaard, P., Jorgensen, R., Whistler, J., and Milligan, G. (2005) A highly conserved glycine within linker I and the extreme C terminus of G protein alpha subunits interact cooperatively in switching G protein-coupled receptor-to-effector specificity. Journal of Pharmacology and Experimental Therapeutics, 313 . pp. 78-87. (doi:10.1124/jpet.104.080424)

Kostenis, E. et al. (2005) (G)-protein-coupled receptor Mas is a physiological antagonist of the angiotensin II type 1 receptor. Circulation, 111 . pp. 1806-1813. (doi:10.1161/01.CIR.0000160867.23556.7D)

Kostenis, E., Waelbroeck, M., and Milligan, G. (2005) Techniques: Promiscuous G alpha proteins in basic research and drug discovery. Trends in Pharmacological Sciences, 26 . pp. 595-602. ISSN 0165-6147 (doi:10.1016/j.tips.2005.09.007)

Milasta, S., Evans, N., Ormiston, L., Wilson, S., Lefkowitz, R., and Milligan, G. (2005) The sustainability of interactions between the orexin-1 receptor and beta-arrestin-2 is defined by a single C-terminal cluster of hydroxy amino acids and modulates the kinetics of ERK MAPK regulation. Biochemical Journal, 387 . pp. 573-584. ISSN 0264-6021

Milligan, G. (2005) Opioid receptors and their interacting proteins. Neuromolecular Medicine, 7 . pp. 51-59. (doi:10.1385/NMM)

Milligan, G., Wilson, S., and Lopez-Gimenez, J. (2005) The specificity and molecular basis of alpha(1)-adrenoceptor and CXCR chemokine receptor dimerization. Journal of Molecular Neuroscience, 26 . pp. 161-167. (doi:10.1385/JMN/26)

Murdoch, H., Feng, G., Bachner, D., Ormiston, L., White, J., Richter, D., and Milligan, G. (2005) Periplakin interferes with g protein activation by the melanin-concentrating hormone receptor-1 by binding to the proximal segment of the receptor C-terminal tail. Journal of Biological Chemistry, 280 . pp. 8208-8220. ISSN 0021-9258 (doi:10.1074/jbc.M405215200)

Pascal, G., and Milligan, G. (2005) Functional complementation and the analysis of opioid receptor homodimerization. Molecular Pharmacology, 68 . pp. 905-915. (doi:10.1124/mol.105.013847)

Pediani, J., Colston, J., Caldwell, D., Milligan, G., Daly, C., and McGrath, J. (2005) beta-arrestin-dependent spontaneous alpha(1a)-adrenoceptor endocytosis causes intracellular transportation of alpha-blockers via recycling compartments. Molecular Pharmacology, 67 . pp. 992-1004. (doi:10.1124/mol.104.008417)

Rudajev, V., Novotny, J., Hejnova, L., Milligan, G., and Svoboda, P. (2005) Dominant portion of thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and G(q)alpha/G(11)alpha protein. Journal of Biochemistry, 138 . pp. 111-125. (doi:10.1093/jb/mvi114)

Ward, R., and Milligan, G. (2005) A key serine for the GTPase-activating protein function of regulator of G protein signaling proteins is not a general target for 14-3-3 interactions. Molecular Pharmacology, 68 . pp. 1821-1830. (doi:10.1124/moi.105.015073)

Wilson, S., Wilkinson, G., and Milligan, G. (2005) The CXCR1 and CXCR2 receptors form constitutive homo- and heterodimers selectively and with equal apparent affinities. Journal of Biological Chemistry, 280 . pp. 28663-28674. ISSN 0021-9258 (doi:10.1074/jbc.M413475200)

Milligan, G., Feng, G.J., Ward, R.J., Sartania, N., Ramsay, D., McLean, A.J., and Carrillo, J.J. (2004) G protein-coupled receptor fusion proteins in drug discovery. Current Pharmaceutical Design, 10 (17). pp. 1989-2001. ISSN 1381-6128 (doi:10.2174/1381612043384295)

Bakker, R., Dees, G., Carrillo, J., Booth, R., Lopez-Gimenez, J., Milligan, G., Strange, P., and Leurs, R. (2004) Domain swapping in the human histamine H-1 receptor. Journal of Pharmacology and Experimental Therapeutics, 311 . pp. 131-138. (doi:10.1124/jpet.104.067041)

Carrillo, J., Lopez-Gimenez, J., and Milligan, G. (2004) Multiple interactions between transmembrane helices generate the oligomeric alpha(1b)-adrenoceptor. Molecular Pharmacology, 66 . pp. 1123-1137. (doi:10.1124/mol.104.001586)

Heydorn, A., Ward, R., Jorgensen, R., Rosenkilde, M., Frimurer, T., Milligan, G., and Kostenis, E. (2004) Identification of a novel site within G protein alpha subunits important for specificity of receptor-G protein interaction. Molecular Pharmacology, 66 . pp. 250-259.

Holdsworth, G., Osborne, D.A., Thi Pham, T., Fells, J.I., Hutchinson, G., Milligan, G., and Parrill, A.L. (2004) A single amino acid determines preference between phospholipids and reveals length restriction for activation ofthe S1P4 receptor. BMC Biochemistry, 5 (12). ISSN 1471-2091 (doi:10.1186/1471-2091-5-12)

Klaasse, E., de Ligt, R., Roerink, S., Lorenzen, A., Milligan, G., Leurs, R., and IJzerman, A. (2004) Allosteric modulation and constitutive activity of fusion proteins between the adenosine A(1) receptor and different (351)Cys-mutated G(i) alpha-subunits. European Journal of Pharmacology, 499 . pp. 91-98. (doi:10.1016/j.ejphar.2004.07.108)

Milligan, G. (2004) Applications of bioluminescence- and fluorescence resonance energy transfer to drug discovery at G protein-coupled receptors. European Journal of Pharmaceutical Sciences, 21 . pp. 397-405. (doi:10.1016/j.ejps.2003.11.010)

Milligan, G. (2004) G protein-coupled receptor dimerization: Function and ligand pharmacology. Molecular Pharmacology, 66 . pp. 1-7. (doi:10.1124/mol.104.000497)

Milligan, G. (2004) A single amino acid determines preference between phospholipids and reveals length restriction for activation ofthe S1P4 receptor. BMC Biochemistry, 5 (12). pp. 1-12. ISSN 1471-2091 (doi:10.1186/1471-2091-5-12)

Milligan, G., Lopez-Gimenez, J., Wilson, S., and Carrillo, J. (2004) Selectivity in the oligomerisation of G protein-coupled receptors. Seminars in Cell and Developmental Biology, 15 . pp. 263-268. (doi:10.1016/j.semcdb.2003.12.012)

Milligan, G., Murdoch, H., Kellett, E., White, J., and Feng, G. (2004) Interactions between G-protein-coupled receptors and periplakin: a selective means to regulate G-protein activation. Biochemical Society Transactions, 32 . pp. 878-880.

Milligan, G., Pascal, G., and Carrillo, J. (2004) Monitoring receptor dimerisation. Medicinal Chemistry Research, 13 . pp. 18-24.

Milligan, G., Pediani, J., Fidock, M., and Lopez-Gimenez, J. (2004) Dimerization of alpha(1)-adrenoceptors. Biochemical Society Transactions, 32 . pp. 847-850.

Moravcova, Z., Rudajev, V., Stohr, J., Novotny, J., Cerny, J., Parenti, M., Milligan, G., and Svoboda, P. (2004) Long-term agonist stimulation of IP prostanoid receptor depletes the cognate G(s)alpha protein in membrane domains but does not change the receptor level. Biochimica et Biophysica Acta: Molecular Cell Research, 1691 . pp. 51-65. ISSN 0167-4889 (doi:10.1016/j.bbamcr.2003.12.004)

Ramsay, D., Carr, I., Pediani, J., Lopez-Gimenez, J., Thurlow, R., Fidock, M., and Milligan, G. (2004) High-affinity interactions between human alpha(1A)-adrenoceptor C-terminal splice variants produce homo- and heterodimers but do not generate the alpha(1L)-adrenoceptor. Molecular Pharmacology, 66 . pp. 228-239.

Ward, R.J., and Milligan, G. (2004) Analysis of function of receptor-G-protein and receptor-RGS fusion proteins. Methods in Molecular Biology, 259 . pp. 225-247. ISSN 1064-3745

Adie, E., Francis, M., Davies, J., Smith, L., Marenghi, A., Hather, C., Hadingham, K., Michael, N., Milligan, G., and Gamel, S. (2003) CypHer 5: A generic approach for measuring the activation and trafficking of G protein-coupled receptors in live cells. Assay and Drug Development Technologies, 1 . pp. 251-259.

Bahia, D. S., Sartania, N., Ward, R. j., Cavalli, A., Jones, T. L.Z., Druey, K. M., and Milligan, G. (2003) Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity o. Journal of Neurochemistry, 85 (5). pp. 1289-1298. ISSN 0022-3042 (doi:10.1046/j.1471-4159.2003.01769.x)

Benians, A., Leaney, J., Milligan, G., and Tinker, A. (2003) The dynamics of formation and action of the ternary complex revealed in living cells using a G-protein-gated K+ channel as a biosensor. Journal of Biological Chemistry, 278 . pp. 10851-10858. ISSN 0021-9258 (doi:10.1074/jbc.M212299200)

Bertaso, F., Ward, R., Viard, P., Milligan, G., and Dolphin, A. (2003) Mechanism of action of G(q) to inhibit G beta gamma modulation of Ca(v)2.2 calcium channels: Probed by the use of receptor-G alpha tandems. Molecular Pharmacology, 63 . pp. 832-843.

Bourova, L., Kostrnova, A., Hejnova, L., Moravcova, Z., Moon, H., Novotny, J., Milligan, G., and Svoboda, P. (2003) delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains. Journal of Neurochemistry, 85 . pp. 34-49. (doi:10.1046/j.1471-4159.2003.01667.x)

Carrillo, J., Pediani, J., and Milligan, G. (2003) Dimers of class A G protein-coupled receptors function via agonist-mediated trans-activation of associated G proteins. Journal of Biological Chemistry, 278 . pp. 42578-42587. ISSN 0021-9258 (doi:10.1074/jbc.M306165200)

Derrien, A., Zheng, B., Osterhout, J., Ma, Y., Milligan, G., Farquhar, M., and Druey, K. (2003) Src-mediated RGS16 tyrosine phosphorylation promotes RGS16 stability. Journal of Biological Chemistry, 278 . pp. 16107-16116. ISSN 0021-9258 (doi:10.1074/jbc.M210371200)

Feng, G., Kellett, E., Scorer, C., Wilde, J., White, J., and Milligan, G. (2003) Selective interactions between helix VIII of the human mu-opioid receptors and the C terminus of periplakin disrupt G protein activation. Journal of Biological Chemistry, 278 . pp. 33400-33407. ISSN 0021-9258 (doi:10.1074/jbc.M305866200)

Hiol, A., Davey, P., Osterhout, J., Waheed, A., Fischer, E., Chen, C., Milligan, G., Druey, K., and Jones, T. (2003) Palmitoylation regulates regulators of G-protein signaling (RGS) 16 function - I. Mutation of amino-terminal cysteine residues on RGS16 prevents its targeting to lipid rafts and palmitoylation of an internal cysteine residue. Journal of Biological Chemistry, 278 . pp. 19301-19308. ISSN 0021-9258 (doi:10.1074/jbc.M210123200)

Milligan, G. (2003) Constitutive activity and inverse agonists of G protein-coupled receptors: a current perspective. Molecular Pharmacology, 64 . pp. 1271-1276.

Milligan, G. (2003) High-content assays for ligand regulation of G-protein-coupled receptors. Drug Discovery Today, 8 . pp. 579-585.

Milligan, G. (2003) Mechanism of action of Gq to inhibit Gbg modulation of Cav2.2 calcium channls probed by use of receptor-Ga tandems. Molecular Pharmacology, 63 (4). pp. 832-843. (doi:10.1124/mol.63.4.832)

Milligan, G. (2003) Principles: Extending the utility of [S-35]GTP gamma S binding assays. Trends in Pharmacological Sciences, 24 . pp. 87-90. ISSN 0165-6147

Milligan, G., Ramsay, D., Pascal, G., and Carrillo, J. (2003) GPCR dimerisation. Life Sciences, 74 . pp. 181-188. (doi:10.1016/j.lfs.2003.09.005)

Osterhout, J., Waheed, A., Hiol, A., Ward, R., Davey, P., Nini, L., Wang, J., Milligan, G., Jones, T., and Druey, K. (2003) Palmitoylation regulates regulator of G-protein signaling (RGS) 16 function - II. Palmitoylation of a cysteine residue in the RGS box is critical for RGS16 GTPase accelerating activity and regulation of G(i)-coupled signaling. Journal of Biological Chemistry, 278 . pp. 19309-19316. ISSN 0021-9258 (doi:10.1074/jbc.M210124200)

Zeng, F., McLean, A., Milligan, G., Lerner, M., Chalmers, D., and Behan, D. (2003) Ligand specific up-regulation of a Renilla reniformis luciferase-tagged, structurally unstable muscarinic M-3 chimeric G protein-coupled receptor. Molecular Pharmacology, 64 . pp. 1474-1484.

Adamson, P., Wilbourn, B., Etienne-Manneville, S., Calder, V., Beraud, E., Milligan, G., Couraud, P., and Greenwood, J. (2002) Lymphocyte trafficking through the blood-brain barrier is dependent on endothelial cell heterotrimeric G-protein signaling. FASEB Journal, 16 .

Adie, E., Kalinka, S., Smith, L., Francis, M., Marenghi, A., Cooper, M., Briggs, M., Michael, N., Milligan, G., and Game, S. (2002) A pH-sensitive fluor, CypHer (TM) 5, used to monitor agonist-induced g protein-coupled receptor internalization in live cells. Biotechniques, 33 . pp. 1152-1157.

Carrillo, J., Stevens, P., and Milligan, G. (2002) Measurement of agonist-dependent and -independent signal initiation of alpha(1b)-adrenoceptor mutants by direct analysis of guanine nucleotide exchange on the G protein G alpha(11). Journal of Pharmacology and Experimental Therapeutics, 302 . pp. 1080-1088. (doi:10.1124/jpet.102.035501)

Feng, G., Cavalli, A., and Milligan, G. (2002) Engineering a V-2 vasopressin receptor agonist- and regulator of G-protein-signaling-sensitive G protein. Analytical Biochemistry, 300 . pp. 212-220. (doi:10.1006/abio.2001.5448)

Liu, S., Carrillo, J., Pediani, J., and Milligan, G. (2002) Effective information transfer from the alpha(1b)-adrenoceptor to G alpha(11) requires both beta/gamma interactions and an aromatic group four amino acids from the C terminus of the G protein. Journal of Biological Chemistry, 277 . pp. 25707-25714. ISSN 0021-9258 (doi:10.1074/jbc.M201015200)

Lyall, F., Lye, S., Teoh, T., Cousins, F., Milligan, G., and Robson, S. (2002) Expression of Gs alpha, connexin-43, connexin-26, and EP1, 3, and 4 receptors in myometrium of prelabor singleton versus multiple gestations and the effects of mechanical stretch and steroids on Gs alpha. Journal of the Society For Gynecologic Investigation, 9 . pp. 299-307.

Massotte, D., Brillet, K., Kieffer, B., and Milligan, G. (2002) Agonists activate G(i1)alpha or G(i2)alpha fused to the human mu opioid receptor differently. Journal of Neurochemistry, 81 . pp. 1372-1382.

McLean, A., Zeng, F., Behan, D., Chalmers, D., and Milligan, G. (2002) Generation and analysis of constitutively active and physically destabilized mutants of the human beta(1)-adrenoceptor. Molecular Pharmacology, 62 . pp. 747-755.

Milligan, G. (2002) Construction and analysis of function of G protein-coupled receptor-G protein fusion proteins. G Protein Pathways, Pt A, Receptors, 343 . pp. 260-273.

Milligan, G. (2002) Strategies to identify ligands for orphan G-protein-coupled receptors. Biochemical Society Transactions, 30 . pp. 789-793.

Milligan, G. (2002) The use of receptor G-protein fusion proteins for the study of ligand activity. Receptors and Channels, 8 . pp. 309-317. (doi:10.1080/10606820290005281)

Milligan, G., Stevens, P., Ramsay, D., and McLean, A. (2002) Ligand rescue of constitutively active mutant receptors. Neurosignals, 11 . pp. 29-33.

Ramsay, D., Kellett, E., McVey, M., Rees, S., and Milligan, G. (2002) Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less. Biochemical Journal, 365 . pp. 429-440. ISSN 0264-6021 (doi:10.1042/BJ20020251)

Ward, R., and Milligan, G. (2002) Reciprocal mutations of highly conserved residues in transmembrane helices 2 and 7 of the alpha(2A)-adrenoceptor restore agonist activation of G(i1 alpha). Cellular Signalling, 14 . pp. 139-144.

Welsby, P., Carr, I., Wilkinson, G., and Milligan, G. (2002) Regulation of the avidity of ternary complexes containing the human 5-HT1A receptor by mutation of a receptor contact site on the interacting G protein alpha subunit. British Journal of Pharmacology, 137 . pp. 345-352. (doi:10.1038/sj.bjp.0704880)

Welsby, P., Kellett, E., Wilkinson, G., and Milligan, G. (2002) Enhanced detection of receptor constitutive activity in the presence of regulators of G protein signaling: Applications to the detection and analysis of inverse agonists and low-efficacy partial agonists. Molecular Pharmacology, 61 . pp. 1211-1221.

Milligan, G., and White, J. H. (2001) Protein-protein interactions at G-protein-coupled receptors. Trends in Pharmacological Sciences, 22 (10). pp. 513-518. ISSN 0165-6147 (doi:10.1016/S0165-6147(00)01801-0)

Evans, N., Groarke, D., Warrack, J., Greenwood, C., Dodgson, K., Milligan, G., and Wilson, S. (2001) Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. Journal of Neurochemistry, 77 . pp. 476-485.

Fernandez-Fernandez, J., Abogadie, F., Milligan, G., Delmas, P., and Brown, D. (2001) Multiple pertussis toxin-sensitive G-proteins can couple receptors to GIRK channels in rat sympathetic neurons when expressed heterologously, but only native G(i)-proteins do so in situ. European Journal of Neuroscience, 14 . pp. 283-292.

Groarke, D., Drmota, T., Bahia, D., Evans, N., Wilson, S., and Milligan, G. (2001) Analysis of the C-terminal tail of the rat thyrotropin-releasing hormone receptor-1 in interactions and cointernalization with beta-arrestin 1-green fluorescent protein. Molecular Pharmacology, 59 . pp. 375-385.

Hoffmann, M., Ward, R., Cavalli, A., Carr, I., and Milligan, G. (2001) Differential capacities of the RGS1, RGS16 and RGS-GAIP regulators of G protein signaling to enhance alpha(2A)-adrenoreceptor agonist-stimulated GTPase activity of G(o1)alpha. Journal of Neurochemistry, 78 . pp. 797-806.

McVey, M., Ramsay, D., Kellett, E., Rees, S., Wilson, S., Pope, A., and Milligan, G. (2001) Monitoring receptor oligomerization using time-resolved fluorescence resonance energy transfer and bioluminescence resonance energy transfer - The human delta-opioid receptor displays constitutive oligomerization at the cell surface, which is not regulate. Journal of Biological Chemistry, 276 . pp. 14092-14099. ISSN 0021-9258

Millan, M. et al. (2001) Antiparkinsonian agent piribedil displays antagonist properties at native, rat, and cloned, human alpha(2)-adrenoceptors: Cellular and functional characterization. Journal of Pharmacology and Experimental Therapeutics, 297 . pp. 876-887.

Milligan, G. (2001) Oligomerisation of G-protein-coupled receptors. Journal of Cell Science, 114 . pp. 1265-1271.

Milligan, G. (2001) The human d opioid receptor activates Gi1a more efficiently than Go1a. Journal of Neurochemistry, 76 (6). pp. 1805-1813. (doi:10.1046/j.1471-4159.2001.00196.x)

Milligan, G., Kellett, E., Dacquet, C., Dubreuil, V., Jacoby, E., Millan, M., Lavielle, G., and Spedding, M. (2001) S 14506: novel receptor coupling at 5-HT1A receptors. Neuropharmacology, 40 . pp. 334-344.

Moon, H., Bahia, D., Cavalli, A., Hoffmann, M., and Milligan, G. (2001) Control of the efficiency of agonist-induced information transfer and stability of the ternary complex containing the delta opioid receptor and the alpha subunit of G(i1) by mutation of a receptor/G protein contact interface. Neuropharmacology, 41 . pp. 321-330.

Moon, H., Cavalli, A., Bahia, D., Hoffmann, M., Massotte, D., and Milligan, G. (2001) The human delta opioid receptor activates G(i1)alpha more efficiently than G(o1)alpha. Journal of Neurochemistry, 76 . pp. 1805-1813.

Ramsay, D., Bevan, N., Rees, S., and Milligan, G. (2001) Detection of receptor ligands by monitoring selective stabilization of a Renilla luciferase-tagged, constitutively active mutant, G-protein-coupled receptor. British Journal of Pharmacology, 133 . pp. 315-323.

Stevens, P., Pediani, J., Carrillo, J., and Milligan, G. (2001) Coordinated agonist regulation of receptor and G protein palmitoylation and functional rescue of palmitoylation-deficient mutants of the G protein G(11)alpha following fusion to the alpha(1b)-adrenoreceptor - Palmitoylation of G(11 alpha) is not required. Journal of Biological Chemistry, 276 . pp. 35883-35890. ISSN 0021-9258

Book Section

Hudson, B.D., Smith, N.J., and Milligan, G. (2011) Experimental challenges to targeting poorly characterized GPCRs: uncovering the therapeutic potential for free fatty acid receptors. In: Neubig, R.R. (ed.) Pharmacology of G Protein Coupled Receptors. Series: Advances in Pharmacology (62). Elsevier Academic Press, Amsterdam, The Netherlands, pp. 175-218. ISBN 9780123859525

Saenz del Burgo, L., and Milligan, G. (2011) Ligand regulation of GPCR quaternary structure. In: Giraldo, J. and Pin, J.-P. (eds.) G Protein-Coupled Receptors: From Structure to Function G Protein-Coupled Receptors: From Structure to Function. Series: RSC drug discovery (8). RSC Publishing, Cambridge, UK, pp. 111-152. ISBN 9781849733441

Appelbe, S., and Milligan, G. (2009) Hetero‐Oligomerization of Chemokine Receptors. In: Chemokines, Part B. Series: Methods in Enzymology. , 461 . Academic Press, pp. 207-225. ISBN 9780123749079

Milligan, G., and Vanderbauwhede, W. (2007) Implementation of finite state machines on a reconfigurable device. In: Arslan, T. (ed.) Second NASA/ESA Conference on Adaptive Hardware and Systems (AHS 2007), 5-8 Aug. 2007, Edinburgh, UK. IEEE Computer Society, Los Alamitos, USA, pp. 386-396. ISBN 9780769528663

Patent

MX PA/A/2005/012546 (2011) Materials and Methods Relating to G-Protein Coupled Oligomers. .

US 10/555,709 (2011) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

US 12/119,401 (2011) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

CN 200480017258.7 (2011) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

TW 093114280 (2011) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

US 09/913, 762 (2011) Receptor Assay. .

PA/A/2005/012546 (2010) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

10/555709 (2008) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

093114280 (2004) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

60/472025 (2003) Materials and Methods Relating to G-Protein Coupled Receptor Oligomers. .

This list was generated on Wed Oct 1 14:11:39 2014 BST.

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • The organisational structure of class A GPCRs: Implications for pharmacology, function and therapeutic regulation
    Medical Research Council
    2014 - 2019
     
  • Designer Receptor Exclusively Activated by Designer Drug' to define the role of short chain fatty acids in metabolic disease and inflammation (Fatty acid DREADD)
    Biotechnology and Biological Sciences Research Council
    2014 - 2018
     
  • Production of pan-species antagonists and validation of GPR43 as an ant-inflammatory and anti-diabetic target
    Scottish Universities Life Sciences Alliance
    2014 - 2015
     
  • Angiotensin-(1-7) and angiotensin-(1-9): assessment as therapeutic targets in acute vascular injury and remodelling.
    Medical Research Council
    2014 - 2017
     
  • GPR120: a G protein-coupled receptor with the potential to regulate insulin secretion and inflammation
    Biotechnology and Biological Sciences Research Council
    2013 - 2017
     
  • FFARMED - The molecular effects of food on metabolic diseases through nutrient sensing free fatty acid receptors
    Danish Agency for Science, Technology, and Innovation
    2012 - 2016
     
  • Identification of novel FFA1 and GPR120 ligands and their use in elucidating the pharmacology, function, and clinical utility of these receptors in the treatment of obesity and diabetes
    Canadian Institutes of Health Research
    2011 - 2014
     
  • Characterisation of T16, a novel stem cell survival compound (ISSF Catalyst Fund)
    Wellcome Trust
    2011 - 2014
     
  • RERAD Food and Drink Strategic Partnership
    Scottish Government
    2011 - 2016
     
  • Red blood cell programme
    Scottish Funding Council
    2011 - 2011
     
  • Red blood cell programme
    Scottish Funding Council
    2011 - 2011
     
  • Developing selective ligands for Free Fatty Acid Receptor 2 with novel pharmacological properties
    Wellcome Trust
    2010 - 2012
     
  • Dissecting the mechanism of action of the novel renin angiotensin hormone angiotensin1-9
    MRC
    2010 - 2012
     
  • The organisational structure of class A GPCRs: implications for function and drug design
    MRC
    2009 - 2014
     
  • Interrogation and manipulation of micro RNA during differentiation of human ES cells to cardiomyocyte and vascular lineages
    British Heart Foundation
    2009 - 2012
     
  • Molecular basis of the functional regulation of mu opioid receptors by co-expressed Gq/G11-coupled GPCRs
    BBSRC
    2008 - 2011
     
  • Uncovering the pharmacology of the G-protein coupled reception GPR40
    BBSRC
    2008 - 2011
     
  • Regulation of bone metabolism by cannabinoid receptors: mechanisms of action and implications for the treatment of bone disease
    Arthritis Research Campaign
    2008 - 2008
     
  • Exploring the selectivity and consequences of GPCR homo and hetero dimerisation/oligomerisation using receptors activated solely by ......
    BBSRC
    2007 - 2010
     
  • The molecular basis of cannabinoid CB1 receptor regulation of the sensitivity of orexin-1 receptor signalling
    BBSRC
    2005 - 2009
     
  • The existence and functional importance of interactions between opiod receptors and the CXCR1/2 chemokine receptors
    Wellcome Trust
    2005 - 2008
     
  • Protein interactions and compartmentalisation in cell signalling
    MRC
    2005 - 2008
     
  • The quaternary structure of G-protein coupled receptors - implications for function and drug design.
    MRC
    2004 - 2009
     
  • Mechanisms and basis of G protein-indepedent signal transduction by the orexin-1 receptor
    Wellcome Trust
    2004 - 2007
     
  • H1R Signalling and Immune Deviation in EAE
    NIH via University of Vermont & State Agricultural College
    2003 - 2005
     
  • Subcellular compartmentalisation analysis by confocal microscopy
    BBSRC
    2003 - 2007
     
  • The interactions between G11 alpha and regulators of G protein signalling and the mechanisms of receptor dimerisation monitored by single..
    Wellcome Trust
    2003 - 2006
     
  • Post transitional modification and the function of regulator of G protien signalling
    BBSRC
    2003 - 2006
     
  • The specificity and basis of GPRC hetero-oligomerisation
    BBSRC
    2003 - 2006
     

Editorial Board

  • 2004 - ongoing: BMC Cell Biology
  • 1996 - 1999: British Journal of Pharmacology
  • 1995 - ongoing: Molecular Pharmacology
  • 1995 - 2002: Biochemical Journal
  • 1989 - 2002: Biochemical Pharmacology

Grant Advisory Board

  • 2010 - ongoing: Anchor Therapeutics - Member of Scientific Advisory Board (http://www.anchortx.com/)
  • 2007 - 2011: Heptares Therapeutics Limited - Member of Scientific Advisory Board. http://www.heptares.com/
  • 2006 - ongoing: Faculty of 1000 - Section Head 'Molecular Pharmacology'
  • 2005 - ongoing: IUPHAR - Member Nomenclature committee; GPCR dimer committee
  • 2005 - ongoing: Cara Therapeutics - Member of Scientific Advisory Board. http://www.caratherapeutics.com/
  • 2004 - ongoing: MRC - Member of College of Experts, Molecular and Cellular Medicine Board.
  • 2003 - ongoing: Caledonian Research Foundation - Biomedical Research Fellowship Committee for the RSE
  • 2001 - 2004: Wellcome Trust - Physiology & Pharmacology Panel
  • 1998 - 2004: MRC - Advisory Board
  • 1995 - 1998: MRC - PMIB "A"

Invited International Presentations

  • 2011: Berlin, Germany - 9th Annual IIR GPCR meeting, Keynote presentation
  • 2011: Istanbul, Turkey - EHPAR symposium
  • 2011: Manchester, UK - 5th ELRIG Drug Discovery meeting,
  • 2011: Ventura, CA USA - Gordon Research Conference 'Molecular Pharmacology'
  • 2011: Bonn, Germany - 'Integrated Pharmacology: Signaling from Cells to Systems'
  • 2011: Indianapolis, IN, USA - Lilly Research Laboratories
  • 2011: Istanbul, Turkey - EHPAR symposium at Turkish Pharmacological Society meeting
  • 2011: Washington DC, USA - American Society for Experimental Pharmacology and Therapeutics, symposium at Experimental Biology
  • 2010: Boston, MA, USA - 2010 Pepducin Science Symposium
  • 2010: Uppsala University, Sweden - The Svedberg Lecture Series
  • 2010: Toronto, Canada - 11th annual Great Lakes GPCR retreat, Keynote Address: The Hyman Niznik Memorial Lecture
  • 2010: Barcelona, Spain - Screening Europe 2010, 'Keynote' lecture 'What are the right assays to select?'
  • 2010: Berlin, Germany - Keynote Presentation: 8th Annual 'G Protein-Coupled Receptors in Drug Discovery' meeting. 'Cutting edge developments in GPCR science and their impact on drug discovery'
  • 2010: Boston, MA, USA - GPCR-based Drug Discovery: Designing and Optimizing Therapies Targeting GPCRs
  • 2010: Nottingham, UK - Systems biology of GPCR signalling' - Plenary Lecture
  • 2010: Montreux Switzerland - 2nd Annual GPCRs Congress
  • 2010: Helsinki, Finland - GPCRs 2010
  • 2009: Graz, Austria - Austrian Pharmacological Society, The EPHAR lecture.
  • 2009: Barga, Italy - Programme Chair, 'Gordon' Conference, Molecular Pharmacology
  • 2009: Portland, USA - Programme Chair, 35th International Narcotics Research Conference
  • 2009: Wuerzburg, Germany - GPCR dimerisation meeting
  • 2009: Lille, France - Society for Biomolecular Sciences, Bioassay & Technology Innovation: 15 Years of Shaping Drug Discovery
  • 2009: Boston, MA, USA - Keynote presentation, Cambridge Healthtech Institute 'Discovery on Target' GPCR-based drug discovery
  • 2009: GSK, Stevenage, UK - Biochemical Society Focused meeting 'Revolutionising Drug Discovery with Stem Cell Technology'
  • 2009: Noordwijkerhout, Holland - Programme Chair, Noordwijkerhout Camerino-Cyprus Symposium 'Trends in Drug Research'
  • 2009: Barcelona, Spain - 7th IBC/IIR Annual GPCR meeting
  • 2009: Leicester, U.K. - 3rd British Pharmacological Society Focused Meeting on Cell Signalling
  • 2009: Lille, France - Programme Chair, GPCR Drug Discovery Symposium
  • 2009: Barcelona, Spain - Chairperson of the 7th Annual Congress: G Protein-Coupled Receptors in Drug Discovery. Keynote presentation, 'Orthosteric and allosteric control of the free fatty acid receptors FFA2 and FFA3',
  • 2008: Berlin, Germany - 6th Annual G protein-coupled receptors in drug discovery
  • 2008: Federation of EuropeanPharmacological Societies (EPHAR), - Manchester, U.K.
  • 2008: Barcelona, Spain - European College of Neuropsychopharmacology
  • 2008: San Diego, CA, USA - American Society of Pharmacology and Experimental Therapeutics (ASPET) 100th Anniversary meeting
  • 2007: Melbourne, Australia - Molecular pharmacology of G protein-coupled receptors
  • 2007: Leicester, England - British Society for Pharmacology, Cell Signalling meeting,
  • 2007: Ridgefield, CT, USA. - Boehringer Ingelheim
  • 2007: Lisbon, Portugal - Keynote Presentation IIR GPCR meeting
  • 2007: Boston, MA, USA - Genzyme
  • 2007: Leiden, Holland - University of Leiden
  • 2007: Southampton, England - Ferring Pharmaceuticals, Symposium on G-protein coupled receptors.
  • 2007: Biddeford, Maine, USA. - Gordon Research Conference on "Phosphorylation and G Protein Mediated Signaling Networks", University of New England
  • 2007: Lexington, MA, USA - EPIX Pharmaceuticals
  • 2007: Barcelona, Spain - Keynote Presentation, 'Novel Assay Technologies for the Discovery of GPCR Drugs", Screening Europe Conference
  • 2007: Glasgow, Scotland - LifeSciences 2007
  • 2007: Prague, Czech Republic. - 6th International Conference of the Czech Neuroscience Society
  • 2006: Keystone, CO, USA - Co-chair and co-organiser of Keystone Conference on G protein coupled receptors. Evolving concepts and drug discovery.
  • 2006: La Jolla, CA, USA - Plenary Lecture - CHI Drug Discovery Chemistry, G protein coupled receptor drug discovery
  • 2006: Glasgow, Scotland - 8th European Congress of Endocrinology,
  • 2006: Santiago de Compostela, Spain - Plenary Lecture Spanish Society of Pharmacology
  • 2006: Stockholm, Sweden - Nordic GPCR meeting 'G protein-coupled receptors-From molecular aspects to novel therapy'. Karolinska Institute, Nobel Forum
  • 2006: Fort Lauderdale, FL, USA - Association for Research in Vision and Ophthalmology, 'Rhodopsin, advances and perspectives'.
  • 2006: Oss, The Netherlands - Organon plc.
  • 2006: Sarasota, FL, USA - Association for Chemoreception Sciences.
  • 2006: Istanbul, Turkey - Plenary lecture: 19th International Symposium on Medicinal Chemistry
  • 2006: Berlin, Germany - Ernst Schering Foundation Symposium on 'G protein-coupled receptors: from deorphanisation to lead structure identification'.
  • 2006: Frankfurt, Germany - Plenary Lecture at GDCh medicinal chemistry meeting.
  • 2006: Barcelona, Spain - Plenary Lecture at 4th IIR Annual GPCR Congress.
  • 2006: Lunteren, The Netherlands - Ariens Award Lecture, Dutch Pharmacological Society
  • 2006: Biberach, Germany - Boehringer-Ingelheim
  • 2006: Las Vegas, Nevada, USA - Plenary Lecture, 11th IBC 'G protein coupled receptor meeting'.
  • 2006: Beijing, China - Speaker and Chair of the Symposium' Frontiers in G-Protein Coupled Receptor Research' - 15th International Union of Pharmacology (IUPHAR)
  • 2005: Amsterdam, Holland - 3rd Annual European Conference on G Protein Coupled Receptors in Drug Discovery
  • 2005: Hilton Waikoloa Village, Waikoloa, Hawaii, USA - 44th Annual American College of Neuropsychopharmacology meeting.
  • 2005: Washington DC Convention Centre, Washington DC, USA - PharmaDiscovery 2005
  • 2005: Bellvue, Washington State, USA - Plenary Lecture at 10th IBC G protein-coupled receptor meeting.
  • 2005: Amsterdam, Holland - Plenary Lecture at 3rd Annual European Conference on G Protein Coupled Receptors in Drug Discovery
  • 2005: Boston, MA, USA - 'The Health of Nations'. Scotland and America-defining Life Science Partnerships. 'Tartan Day' 2005
  • 2005: Washington DC, USA - PharmaDiscovery, Washington DC Convention Centre
  • 2005: Leicester, UK - Plenary Lecture, BPS 'Cell Signalling meeting'
  • 2005: Philadelphia PA, USA - Merck GPCR symposium 'GPCR dimerization: implications for pharmacology and function'
  • 2004: Glasgow, UK. - BioScience 2004. From molecules to organisms.
  • 2004: Stockholm, Sweden. - International Wenner-Gren Symposium 'Receptor-receptor interactions among heptaspanning membrane receptors: From structure to function'
  • 2004: Organon plc Newhouse, UK. - Biochemical Society 'Allosterism & GPCR accessory proteins: new insights into drug discovery'
  • 2004: Frankfurt, Germany. - 16th Friedrich Merz Guest Professorship Symposium. GPCRs as targets for the treatment of CNS diseases.
  • 2004: Taos, New Mexico, USA. - Keystone Conference on G protein coupled receptors.
  • 2004: Copenhagen, Denmark. - 18th International Symposium on Medicinal Chemistry.
  • 2004: San Diego, CA, USA - Plenary Lecture, 9th IBC International G-protein coupled receptors conference
  • 2004: London, UK. - G protein coupled receptors, New approaches to maximise the impact of GPCRs in drug discovery.
  • 2004: Aventis plc, Tucson, Arizona, USA
  • 2004: American Chemical Society, Anaheim, CA, USA.
  • 2004: Genentech, San Francisco, CA, USA.
  • 2004: Amsterdam, The Netherlands. - 2nd annual IIR conference on G Protein Coupled Receptors in Drug Discovery.
  • 2004: Novasite, San Diego, CA, USA.
  • 2004: Kyoto, Japan. - 2nd International Pharmaceutical Sciences World Congress, 'The global translation of science into drug development in advancing therapy'
  • 2004: University of Freiberg, Germany.
  • 2003: Zurich, Switzerland. - Smart Assays for Screening
  • 2003: Strasbourg, France. - G protein coupled receptors in Drug Discovery
  • 2003: Prague, Czech Republic. - 16th European College of Neuropsychopharmacology
  • 2003: Boston, MA, USA. - 8th Annual IBC International Conference on 'G protein-coupled receptors'
  • 2003: Almirall Prodesfarma, Barcelona.
  • 2003: Free University of Amsterdam, Amsterdam, The Netherlands.
  • 2003: Hinxton Hall, Cambridge, UK. - Recent advances in protein kinase and G protein coupled receptor drug discovery.
  • 2003: Camerino, Italy. - 14th Camerino-Noordwijkerhout Symposium
  • 2003: Cologne, Germany. - Cellular Assay Technologies GPCR's * Kinases * Ion Channels * Nuclear Receptors.
  • 2003: Perpignan, France. - International Narcotics Research Conference.
  • 2003: Institute of Physiology, Czech Acamedy of Sciences, Prague, Czech Republic.
  • 2003: San Diego, CA, USA. - Experimental Biology
  • 2003: Fukui, Japan. - 4th International Symposium on receptor mechanisms.
  • 2002: Axaron, Heidelberg, Germany
  • 2002: Cambridge, UK. - 1st British Pharmacological Society
  • 2002: Charles University, Prague, Czech Republic.
  • 2002: San Diego, CA, USA. - 7th Annual IBC International Conference on G protein-coupled receptors.
  • 2002: University of Melbourne, Melbourne, Australia. - Molecular pharmacology of G protein-coupled receptors. The Australasian Society for Clinical and Experimental Pharmacology and Toxocology.
  • 2002: University of Hamburg, Germany.
  • 2002: Berlin Germany. - Assay Development: Developing successful assays and infrastructure for advanced screening in the drug discovery industry.
  • 2002: 7-TM Pharma, Copenhagen, Denmark.
  • 2002: S'Aguru, Spain. - Esteve Foundation meeting on "Inverse Agonism"
  • 2002: Aventis PLC, Frankfurt, Germany.
  • 2001: Western Australian Institute for Medical Research, Perth, Australia.
  • 2001: Laboratory of Allergic Diseases, NIAID/NIH, Bethesda MD, USA
  • 2001: Athens, Greece. - "Demokritos"
  • 2001: Institute of Molecular and Cellular Biology, Singapore.
  • 2001: Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
  • 2001: Novasite Pharmaceuticals, San Diego CA, USA.
  • 2001: Institute of Cellular Signalling, University of Nijmegen, The Netherlands.
  • 2001: Vrije Universiteit, Amsterdam. The Netherlands. - The 1st NAUTA Prize Lecture.
  • 2001: Christchurch, New Zealand. - 34th International Congress of Physiological Sciences: From Molecule to Malady.
  • 2001: Scottsdale, AZ, USA - 6th Annual IBC International Conference on G protein-coupled receptors. New Insights and Drug Discoveries.
  • 2001: Instituto Cajal, Madrid, Spain.
  • 2001: Noordwijkerhout, Holland. - Noordwijkerhout-Camerino Symposium, "Trends in Drug Research"
  • 2001: University of Western Australia, Perth, Australia
  • 2001: Utrecht, The Netherlands - Dutch G protein-coupled receptor symposium.
  • 2001: Orlando FL, USA. - American Society for Pharmacology and Experimental Therapeutics (ASPET) symposium on

Prizes, Awards and Distinctions

  • 2006: Dutch Pharmacological Society 'Ariens' Prize
  • 2001: First Nauta Visiting Professor to the Vrije Universiteit Amsterdam
  • 1998: Poulsson Medal for Pharmacology, Norwegian Society of Pharmacology & Toxicology

Professional Learned Society

  • 1998 - ongoing: Royal Society of Edinburgh - Fellow