Professor Robert Insall
- Professor of Mathematical & Computational Cell Biology (Therapeutic Science Research)
email:
Robert.Insall@glasgow.ac.uk
Beatson Institute, Glasgow G61 1BD
https://orcid.org/0000-0003-4898-040XResearch interests
Link to Cell migration and chemotaxis website
Publications
2023
Dowdell, A., Paschke, P. I., Thomason, P. A., Tweedy, L. and Insall, R. H. (2023) Competition between chemoattractants causes unexpected complexity and can explain negative chemotaxis. Current Biology, 32(9), 1704-1715.e3. (doi: 10.1016/j.cub.2023.03.006) (PMID:37001521)
Insall, R. (2023) Science Twitter — navigating change in science communication. Nature Reviews Molecular Cell Biology, 24, pp. 305-306. (doi: 10.1038/s41580-023-00581-3) (PMID:36732603)
Insall, R. H. (2023) Receptors, enzymes and self-attraction as autocrine generators and amplifiers of chemotaxis and cell steering. Current Opinion in Cell Biology, 81, 102169. (doi: 10.1016/j.ceb.2023.102169) (PMID:37075582)
2022
Singh, S. P. , Paschke, P., Tweedy, L. and Insall, R. H. (2022) AKT and SGK kinases regulate cell migration by altering Scar/WAVE complex activation and Arp2/3 complex recruitment. Frontiers in Molecular Biosciences, 9, 965921. (doi: 10.3389/fmolb.2022.965921) (PMID:36106016) (PMCID:PMC9466652)
Insall, R. H. , Paschke, P. and Tweedy, L. (2022) Steering yourself by the bootstraps: how cells create their own gradients for chemotaxis. Trends in Cell Biology, 32(7), pp. 585-596. (doi: 10.1016/j.tcb.2022.02.007) (PMID:35351380)
Singh, S. P. and Insall, R. H. (2022) Under-agarose chemotaxis and migration assays for dictyostelium. In: Chang, C. and Wang, J. (eds.) Cell Polarity Signaling: Methods and Protocols. Series: Methods in Molecular Biology, 2438. Humana Press: New York, pp. 467-482. ISBN 9781071620342 (doi: 10.1007/978-1-0716-2035-9_27)
2021
Edgar, J. M. , McGowan, E., Chapple, K., Möbius, W., Lemgruber, L. , Insall, R. H. , Nave, K.-A. and Boullerne, A. (2021) Rio-Hortega's drawings revisited with fluorescent proteins define a cytoplasm filled channel system of CNS myelin. Journal of Anatomy, 239(6), pp. 1241-1255. (doi: 10.1111/joa.13577)
Singh, S. P. , Thomason, P. A. and Insall, R. H. (2021) Extracellular signalling modulates Scar/WAVE complex activity through Abi phosphorylation. Cells, 10(12), 3485. (doi: 10.3390/cells10123485) (PMID:34943993) (PMCID:PMC8700165)
Williams, R. S.B., Chubb, J. R., Insall, R. , King, J. S., Pears, C. J., Thompson, E. and Weijer, C. J. (2021) Moving the research forward: the best of British biology using the tractable model system Dictyostelium discoideum. Cells, 10(11), 3036. (doi: 10.3390/cells10113036) (PMID:34831258) (PMCID:PMC8616412)
Yang, Y., Li, D., Chao, X., Singh, S. P. , Thomason, P., Yan, Y., Dong, M., Li, L., Insall, R. H. and Cai, H. (2021) Leep1 interacts with PIP3 and the Scar/WAVE complex to regulate cell migration and macropinocytosis. Journal of Cell Biology, 220(7), e202010096. (doi: 10.1083/jcb.202010096) (PMID:33978708) (PMCID:PMC8127007)
Yelland, T., Le, A. H., Nikolaou, S., Insall, R. , Machesky, L. and Ismail, S. (2021) Structural basis of CYRI-B direct competition with Scar/WAVE complex for Rac1. Structure, 29(3), 226-237.e4. (doi: 10.1016/j.str.2020.11.003) (PMID:33217330) (PMCID:PMC7955166)
Singh, S. P. and Insall, R. H. (2021) Adhesion stimulates Scar/WAVE phosphorylation in mammalian cells. Communicative and Integrative Biology, 14(1), pp. 1-4. (doi: 10.1080/19420889.2020.1855854) (PMID:33447346) (PMCID:PMC7781634)
2020
Zickus, V. et al. (2020) Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation. Scientific Reports, 10, 20986. (doi: 10.1038/s41598-020-77737-0) (PMID:33268900) (PMCID:PMC7710711)
Tweedy, L., Thomason, P. A., Paschke, P. I., Martin, K., Machesky, L. M. , Zagnoni, M. and Insall, R. H. (2020) Seeing around corners: cells solve mazes and respond at a distance using attractant breakdown. Science, 369(6507), eaay9792. (doi: 10.1126/science.aay9792) (PMID:32855311)
Singh, S. P. , Thomason, P. A., Lilla, S., Schaks, M., Tang, Q., Goode, B. L., Machesky, L. M. , Rottner, K. and Insall, R. H. (2020) Cell–substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime. PLoS Biology, 18(8), e3000774. (doi: 10.1371/journal.pbio.3000774) (PMID:32745097) (PMCID:PMC7425996)
McMenamin, P. G., Shields, G. T., Seyed-Razavi, Y., Kalirai, H., Insall, R. H. , Machesky, L. M. and Coupland, S. E. (2020) Melanoblasts populate the mouse choroid earlier in development than previously described. Investigative Ophthalmology and Visual Science, 61(10), 33. (doi: 10.1167/iovs.61.10.33) (PMID:32797202) (PMCID:PMC7441366)
Kadir, S. R., Insall, R. H. , Moffatt, G., McGhee, J. and Livingstone, D. (2020) Analogies in 3D molecular visualisations: development of a cell biology animation ‘How cells move – a new interpretation of old data’. Journal of Visual Communication in Medicine, 43(1), pp. 35-46. (doi: 10.1080/17453054.2019.1671814) (PMID:31642358)
2019
Amato, C., Thomason, P. A., Davidson, A. J., Swaminathan, K., Ismail, S. , Machesky, L. M. and Insall, R. H. (2019) WASP restricts active Rac to maintain cells’ front-rear polarization. Current Biology, 29(24), pp. 4169-4182. (doi: 10.1016/j.cub.2019.10.036) (PMID:31786060) (PMCID:PMC6926487)
Juin, A. et al. (2019) N-WASP control of LPAR1 trafficking establishes response to self-generated LPA gradients to promote pancreatic cancer cell metastasis. Developmental Cell, 51(4), 431-445.e7. (doi: 10.1016/j.devcel.2019.09.018) (PMID:31668663) (PMCID:PMC6863394)
Tweedy, L., Witzel, P., Heinrich, D., Insall, R. H. and Endres, R. G. (2019) Screening by changes in stereotypical behavior during cell motility. Scientific Reports, 9, 8784. (doi: 10.1038/s41598-019-45305-w) (PMID:31217532) (PMCID:PMC6584642)
Buckley, C. M. et al. (2019) PIKfyve/Fab1 is required for efficient V-ATPase and hydrolase delivery to phagosomes, phagosomal killing, and restriction of Legionella infection. PLoS Pathogens, 15(2), e1007551. (doi: 10.1371/journal.ppat.1007551) (PMID:30730983) (PMCID:PMC6382210)
Siebert, S. , Insall, R. and Machesky, L. M. (2019) More means less: managing overflow in science publishing. In: Czarniawska, B. and Löfgren, O. (eds.) Overwhelmed by Overflows? How People and Organizations Create and Manage Excess. Lund University Press: Lund, pp. 130-150. ISBN 9789198469813 (doi: 10.7765/9789198469813.00013)
2018
Schaks, M., Singh, S. P. , Kage, F., Thomason, P., Klünemann, T., Steffen, A., Blankenfeldt, W., Stradal, T. E., Insall, R. H. and Rottner, K. (2018) Distinct interaction sites of Rac GTPase with WAVE regulatory complex have non-redundant functions in vivo. Current Biology, 28(22), 3674-3684.e6. (doi: 10.1016/j.cub.2018.10.002) (PMID:30393033) (PMCID:PMC6264382)
Fort, L. et al. (2018) Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nature Cell Biology, 20(10), pp. 1159-1171. (doi: 10.1038/s41556-018-0198-9) (PMID:30250061) (PMCID:PMC6863750)
Visweshwaran, S. P. et al. (2018) The trimeric coiled‐coil HSBP 1 protein promotes WASH complex assembly at centrosomes. EMBO Journal, 37(13), e97706. (doi: 10.15252/embj.201797706) (PMID:29844016) (PMCID:PMC6028030)
Paschke, P., Knecht, D. A., Silale, A., Traynor, D., Williams, T. D., Thomason, P. A., Insall, R. H. , Chubb, J. R., Kay, R. R. and Veltman, D. M. (2018) Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum. PLoS ONE, 13(5), e0196809. (doi: 10.1371/journal.pone.0196809) (PMID:29847546) (PMCID:PMC5976153)
Pan, M., Neilson, M. P., Grunfeld, A. M., Cruz, P., Wen, X., Insall, R. H. and Jin, T. (2018) A G-protein-coupled chemoattractant receptor recognizes lipopolysaccharide for bacterial phagocytosis. PLoS Biology, 16(5), e2005754. (doi: 10.1371/journal.pbio.2005754) (PMID:29799847) (PMCID:PMC5969738)
Davidson, A. J., Amato, C., Thomason, P. A. and Insall, R. H. (2018) WASP family proteins and formins compete in pseudopod- and bleb-based migration. Journal of Cell Biology, 217(2), pp. 701-714. (doi: 10.1083/jcb.201705160) (PMID:29191847) (PMCID:PMC5800805)
2017
Susanto, O. et al. (2017) LPP3 mediates self-generation of chemotactic LPA gradients by melanoma cells. Journal of Cell Science, 130(20), pp. 3455-3466. (doi: 10.1242/jcs.207514) (PMID:28871044) (PMCID:PMC5665449)
Husmeier, D. , Ferguson, E. , Matthiopoulos, J. and Insall, R. (2017) Statistical Inference of the Drivers of Collective Cell Movement. RSS 2017 Annual Conference, Glasgow, Scotland, 04-07 Sep 2017.
Ferguson, E. A. , Matthiopoulos, J. , Insall, R. H. and Husmeier, D. (2017) Statistical inference of the mechanisms driving collective cell movement. Journal of the Royal Statistical Society: Series C (Applied Statistics), 66(4), pp. 869-890. (doi: 10.1111/rssc.12203)
Thomason, P. A., King, J. S. and Insall, R. H. (2017) Mroh1, a lysosomal regulator localized by WASH-generated actin. Journal of Cell Science, 130(10), pp. 1785-1795. (doi: 10.1242/jcs.197210) (PMID:28424231) (PMCID:PMC5450189)
Periz, J. et al. (2017) Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation. eLife, 6, e24119. (doi: 10.7554/eLife.24119) (PMID:28322189) (PMCID:PMC5375643)
Woodham, E. F. et al. (2017) Coordination by Cdc42 of actin, contractility, and adhesion for melanoblast movement in mouse skin. Current Biology, 27(5), pp. 624-637. (doi: 10.1016/j.cub.2017.01.033) (PMID:28238662) (PMCID:PMC5344686)
2016
Veltman, D. M., Williams, T. D., Bloomfield, G., Chen, B.-C., Betzig, E., Insall, R. and Kay, R. R. (2016) A plasma membrane template for macropinocytic cups. eLife, 5, e20085. (doi: 10.7554/eLife.20085) (PMID:27960076) (PMCID:PMC5154761)
Mackenzie, J.A., Nolan, M. and Insall, R.H. (2016) Local modulation of chemoattractant concentrations by single cells: dissection using a bulk-surface computational model. Interface Focus, 6(5), 20160036. (doi: 10.1098/rsfs.2016.0036) (PMID:27708760) (PMCID:PMC4992739)
Buckley, C. M., Gopaldass, N., Bosmani, C., Johnston, S. A., Soldati, T., Insall, R. and King, J. S. (2016) WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors. Proceedings of the National Academy of Sciences of the United States of America, 113(40), E5906-E5915. (doi: 10.1073/pnas.1524532113) (PMID:27647881) (PMCID:PMC5056073)
Ferguson, E. A. , Matthiopoulos, J. , Insall, R. H. and Husmeier, D. (2016) Inference of the drivers of collective movement in two cell types: Dictyostelium and melanoma. Journal of the Royal Society: Interface, 13(123), 20160695. (doi: 10.1098/rsif.2016.0695) (PMID:27798280)
Tweedy, L., Susanto, O. and Insall, R. H. (2016) Self-generated chemotactic gradients—cells steering themselves. Current Opinion in Cell Biology, 42, pp. 46-51. (doi: 10.1016/j.ceb.2016.04.003) (PMID:27105308)
Tyrrell, B. J., Woodham, E. F., Spence, H. J., Strathdee, D., Insall, R. H. and Machesky, L. M. (2016) Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell and Melanoma Research, 29(5), pp. 559-571. (doi: 10.1111/pcmr.12506) (PMID:27390154) (PMCID:PMC5082549)
Susanto, O., Muinonen-Martin, A. J., Nobis, M. and Insall, R. H. (2016) Visualizing cancer cell chemotaxis and invasion in 2D and 3D. In: Jin, T. and Hereld, D. (eds.) Chemotaxis. Series: Methods in molecular biology, 1407 (1407). Springer, pp. 217-228. ISBN 9781493934782 (doi: 10.1007/978-1-4939-3480-5_17)
Frej, A. D. et al. (2016) The inositol-3-phosphate synthase biosynthetic enzyme has distinct catalytic and metabolic roles. Molecular and Cellular Biology, 36(10), pp. 1464-1479. (doi: 10.1128/mcb.00039-16) (PMID:26951199) (PMCID:PMC4859692)
Tweedy, L., Knecht, D. A., Mackay, G. N. and Insall, R. H. (2016) Self-generated chemoattractant gradients: attractant depletion extends the range and robustness of chemotaxis. PLoS Biology, 14(3), e1002404. (doi: 10.1371/journal.pbio.1002404) (PMID:26981861) (PMCID:PMC4794234)
MacDonald, G., Mackenzie, J.A., Nolan, M. and Insall, R.H. (2016) A computational method for the coupled solution of reaction–diffusion equations on evolving domains and manifolds: application to a model of cell migration and chemotaxis. Journal of Computational Physics, 309, pp. 207-226. (doi: 10.1016/j.jcp.2015.12.038) (PMID:27330221) (PMCID:PMC4896117)
2015
Siebert, S. , Machesky, L. M. and Insall, R. H. (2015) Overflow in science and its implications for trust. eLife, 4, e10825. (doi: 10.7554/eLife.10825)
Muinonen-Martin, A. J. and Insall, R. H. (2015) Melanomas’ fatal attraction to lysophosphatidic acid trails: a new prognostic and therapeutic approach? Melanoma Management, 2(2), pp. 97-100. (doi: 10.2217/mmt.15.11)
Roberts, H. M., Ling, M. R., Insall, R. , Kalna, G., Spengler, J., Grant, M. M. and Chapple, I. L.C. (2015) Impaired neutrophil directional chemotactic accuracy in chronic periodontitis patients. Journal of Clinical Periodontology, 42(1), pp. 1-11. (doi: 10.1111/jcpe.12326) (PMID:25360483) (PMCID:PMC4340045)
Urushihara, H. et al. (2015) Comparative genome and transcriptome analyses of the social amoeba Acytostelium subglobosum that accomplishes multicellular development without germ-soma differentiation. BMC Genomics, 16, 80. (doi: 10.1186/s12864-015-1278-x) (PMID:25758444) (PMCID:PMC4334915)
2014
Muinonen-Martin, A. J. et al. (2014) Melanoma cells break down LPA to establish local gradients that drive chemotactic dispersal. PLoS Biology, 12(10), e1001966. (doi: 10.1371/journal.pbio.1001966) (PMID:25313567) (PMCID:PMC4196730)
Veltman, D.M., Lemieux, M.G., Knecht, D.A. and Insall, R.H. (2014) PIP3-dependent macropinocytosis is incompatible with chemotaxis. Journal of Cell Biology, 204(4), pp. 497-505. (doi: 10.1083/jcb.201309081)
Sapey, E., Greenwood, H., Walton, G., Mann, E., Love, A., Aaronson, N., Insall, R.H. , Stockley, R.A. and Lord, J.M. (2014) Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence. Blood, 123(2), pp. 239-248. (doi: 10.1182/blood-2013-08-519520)
Tyrrell, B. J., Neilson, M., Insall, R. H. and Machesky, L. M. (2014) Predicting cell shapes in melanomas. Pigment Cell and Melanoma Research, 27(1), pp. 5-6. (doi: 10.1111/pcmr.12176) (PMID:24118871)
2013
Davidson, A.J., Ura, S., Thomason, P.A., Kalna, G. and Insall, R.H. (2013) Abi is required for modulation and stability but not localization or activation of the SCAR/WAVE complex. Eukaryotic Cell, 12(11), pp. 1509-1516. (doi: 10.1128/EC.00116-13) (PMID:24036345) (PMCID:PMC3837927)
Insall, R.H. (2013) WASH complex conservation: don’t WASH away the family! Trends in Cell Biology, 23(11), pp. 519-520. (doi: 10.1016/j.tcb.2013.07.009)
Insall, R. (2013) The interaction between pseudopods and extracellular signalling during chemotaxis and directed migration. Current Opinion in Cell Biology, 25(5), pp. 526-531. (doi: 10.1016/j.ceb.2013.04.009)
King, J.S., Gueho, A., Hagedorn, M., Gopaldass, N., Leuba, F., Soldati, T. and Insall, R.H. (2013) WASH is required for lysosomal recycling and efficient autophagic and phagocytic digestion. Molecular Biology of the Cell, 24(17), pp. 2714-2726. (doi: 10.1091/mbc.E13-02-0092)
Davidson, A.J., King, J.S. and Insall, R.H. (2013) The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum. BioTechniques, 55(1), pp. 39-41. (doi: 10.2144/000114054)
Park, L., Thomason, P.A., Zech, T., King, J.S., Veltman, D.M., Carnell, M., Ura, S., Machesky, L.M. and Insall, R.H. (2013) Cyclical Action of the WASH complex: FAM21 and capping protein drive WASH recycling, not initial recruitment. Developmental Cell, 24(2), pp. 169-181. (doi: 10.1016/j.devcel.2012.12.014)
Choi, C.-H., Thomason, P.A., Zaki, M., Insall, R.H. and Barber, D.D.L. (2013) Phosphorylation of actin-related protein 2 (Arp2) is required for normal development and cAMP chemotaxis in dictyostelium. Journal of Biological Chemistry, 288(4), pp. 2464-2474. (doi: 10.1074/jbc.M112.435313)
Tang, H. et al. (2013) Loss of scar/WAVE complex promotes N-WASP- and FAK-dependent invasion. Current Biology, 23(2), pp. 107-117. (doi: 10.1016/j.cub.2012.11.059)
Davidson, A. J. and Insall, R. H. (2013) SCAR/WAVE. Communicative and Integrative Biology, 6(6), e27033. (doi: 10.4161/cib.27033) (PMID:24753786) (PMCID:PMC3984289)
Itakura, A., Aslan, J.E., Kusanto, B.T., Phillips, K.G., Porter, J.E., Newton, P.K., Nan, X., Insall, R.H. , Chernoff, J. and McCarty, O.J.T. (2013) p21-activated kinase (PAK) regulates cytoskeletal reorganization and directional migration in human neutrophils. PLoS ONE, 8(9), e73063. (doi: 10.1371/journal.pone.0073063) (PMID:24019894) (PMCID:PMC3760889)
Muinonen-Martin, A.J., Knecht, D.A., Veltman, D.M., Thomason, P.A., Kalna, G. and Insall, R.H. (2013) Measuring chemotaxis using direct visualization microscope chambers. Methods in Molecular Biology, 1046, pp. 307-321. (doi: 10.1007/978-1-62703-538-5_18)
2012
Shirazi, M. H., Marsh, P., Keane, M. G., Khushal, A. and Insall, R. (2012) Laparoscopic total extra-peritoneal inguinal hernia repairs by a surgeon experienced at laparoscopic cholecystectomy. Journal of the Pakistan Medical Association, 62(12), pp. 1301-1304. (PMID:23866478)
Yu, X. et al. (2012) N-WASP coordinates the delivery and F-actin-mediated capture of MT1-MMP at invasive pseudopods. Journal of Cell Biology, 199(3), pp. 527-544. (doi: 10.1083/jcb.201203025)
Veltman, D. M., King, J. S., Machesky, L. M. and Insall, R. H. (2012) SCAR knockouts in Dictyostelium: WASP assumes SCAR’s position and upstream regulators in pseudopods. Journal of Cell Biology, 198(4), pp. 501-508. (doi: 10.1083/jcb.201205058) (PMID:22891261) (PMCID:PMC3514037)
Pakes, N.K., Veltman, D.M., Rivero, F., Nasir, J., Insall, R. and Williams, R.S.B. (2012) The Rac GEF ZizB regulates development, cell motility and cytokinesis in Dictyostelium. Journal of Cell Science, 125(10), pp. 2457-2465. (doi: 10.1242/jcs.100966)
Ura, S., Pollitt, A.Y., Veltman, D.M., Morrice, N.A., Machesky, L.M. and Insall, R.H. (2012) Pseudopod growth and evolution during cell movement is controlled through SCAR/WAVE dephosphorylation. Current Biology, 22(7), pp. 553-561. (doi: 10.1016/j.cub.2012.02.020)
Insall, R. (2012) How a paper on RAC set the standard. Nature Reviews Molecular Cell Biology, 13(2), p. 66. (doi: 10.1038/nrm3277)
Spence, H.J., Timpson, P., Tang, H.R., Insall, R.H. and Machesky, L.M. (2012) Scar/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions. Biochemical Journal, 448(1), pp. 35-42. (doi: 10.1042/BJ20112206)
2011
King, J.S., Veltman, D.M. and Insall, R.H. (2011) The induction of autophagy by mechanical stress. Autophagy, 7(12), pp. 1490-1499. (doi: 10.4161/auto.7.12.17924)
Li, A. et al. (2011) Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression. Developmental Cell, 21(4), pp. 722-734. (doi: 10.1016/j.devcel.2011.07.008)
Neilson, M. P., Veltman, D. M., van Haastert, P. J. M., Webb, S. D., Mackenzie, J. A. and Insall, R. H. (2011) Chemotaxis: a feedback-based computational model robustly predicts multiple aspects of real cell behaviour. PLoS Biology, 9(5), e1000618. (doi: 10.1371/journal.pbio.1000618)
Sapey, E., Stockley, J. A., Greenwood, H., Ahmad, A., Bayley, D., Lord, J. M., Insall, R. H. and Stockley, R. A. (2011) Behavioral and structural differences in migrating peripheral neutrophils from patients with chronic obstructive pulmonary disease. American Journal of Respiratory and Critical Care Medicine, 183(9), pp. 1176-1186. (doi: 10.1164/rccm.201008-1285OC)
Shirazi, M. H., Tennant, W. G. and Insall, R. (2011) Novel carotid surgery in an asymptomatic totally occluded common carotid artery. Journal of the Pakistan Medical Association, 61(2), pp. 187-189. (PMID:21375176)
Carnell, M., Zech, T., Calaminus, S.D., Ura, S., Hagedorn, M., Johnston, S.A., May, R.C., Soldati, T., Machesky, L.M. and Insall, R.H. (2011) Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis. Journal of Cell Biology, 193(5), pp. 831-839. (doi: 10.1083/jcb.201009119)
Carnell, M.J. and Insall, R.H. (2011) Actin on disease – Studying the pathobiology of cell motility using Dictyostelium discoideum. Seminars in Cell and Developmental Biology, 22(1), pp. 82-88. (doi: 10.1016/j.semcdb.2010.12.003)
Davidson, A. and Insall, R.H. (2011) Actin-Based Motility: WAVE Regulatory Complex Structure Reopens Old SCARs. Current Biology, 21(2), R66-R68. (doi: 10.1016/j.cub.2010.12.001)
Insall, R. (2011) Career postdocs increase scrap heap. Nature, 471(7340), p. 578. (doi: 10.1038/471578e)
Insall, R.H. (2011) Dogma bites back – the evidence for branched actin. Trends in Cell Biology, 21(1), p. 2. (doi: 10.1016/j.tcb.2010.11.002)
Itakura, A., Verbout, N. G., Phillips, K. G., Insall, R.H. , Gailani, D., Tucker, E. I., Gruber, A. and McCarty, O. J. T. (2011) Activated factor XI inhibits chemotaxis of polymorphonuclear leukocytes. Journal of Leukocyte Biology, 90(5), pp. 923-927. (doi: 10.1189/jlb.0411182)
Neilson, M.P., Mackenzie, J.A., Webb, S.D. and Insall, R.H. (2011) Modeling Cell Movement and Chemotaxis Using Pseudopod-Based Feedback. SIAM Journal on Scientific Computing, 33(3), pp. 1035-1057. (doi: 10.1137/100788938)
Veltman, D.W., Auciello, G., Spence, H.J., Machesky, L.M. , Rappoport, J.Z. and Insall, R.H. (2011) Functional analysis of Dictyostelium IBARa reveals a conserved role of the I-BAR domain in endocytosis. Biochemical Journal, 436(1), pp. 45-52. (doi: 10.1042/BJ20101684)
Zech, T., Calaminus, S.D.J., Caswell, P., Spence, H.J., Carnell, M., Insall, R.H. , Norman, J. and Machesky, L.M. (2011) The Arp2/3 activator WASH regulates 5 1-integrin-mediated invasive migration. Journal of Cell Science, 124(22), pp. 3753-3759. (doi: 10.1242/jcs.080986)
2010
Neilson, M. P., Mackenzie, J. A., Webb, S. D. and Insall, R. H. (2010) Use of the parameterised finite element method to robustly and efficiently evolve the edge of a moving cell. Integrative Biology, 2(11-12), pp. 687-695. (doi: 10.1039/c0ib00047g)
Insall, R. H. (2010) Understanding eukaryotic chemotaxis: a pseudopod-centred view. Nature Reviews Molecular Cell Biology, 11(6), pp. 453-458. (doi: 10.1038/nrm2905)
Gullberg, D., Muinonen-Martin, A.J., Veltman, D.M., Kalna, G. and Insall, R. (2010) An improved chamber for direct visualisation of chemotaxis. PLoS ONE, 5(12), e15309. (doi: 10.1371/journal.pone.0015309)
King, J. S., Veltman, D. M., Georgiou, M., Baum, B. and Insall, R. H. (2010) SCAR/WAVE is activated at mitosis and drives myosin-independent cytokinesis. Journal of Cell Science, 123(13), pp. 2246-2255. (doi: 10.1242/jcs.063735)
Veltman, D.M. and Insall, R. H. (2010) WASP Family Proteins: Their Evolution and Its Physiological Implications. Molecular Biology of the Cell, 21(16), pp. 2880-2893. (doi: 10.1091/mbc.E10-04-0372)
2009
Kim, J. and Insall, R. (2009) Position and velocity estimation of cell boundary. In: International Conference on Systems Biology, Palo Alto, USA, 30 Aug - 4 Sep 2009,
Insall, R. and Machesky, L.M. (2009) Actin Dynamics at the Leading Edge: From Simple Machinery to Complex Networks. Developmental Cell, 17(3), pp. 310-322. (doi: 10.1016/j.devcel.2009.08.012)
King, J.S. and Insall, R.H. (2009) Chemotaxis: finding the way forward with Dictyostelium. Trends in Cell Biology, 19(10), pp. 523-530. (doi: 10.1016/j.tcb.2009.07.004)
Pollitt, A. Y. and Insall, R. H. (2009) WASP and SCAR/WAVE proteins: the drivers of actin assembly. Journal of Cell Science, 122(15), pp. 2575-2578. (doi: 10.1242/jcs.023879)
Pollitt, A.Y. and Insall, R.H. (2009) Loss of Dictyostelium HSPC300 causes a scar-like phenotype and loss of SCAR protein. BMC Cell Biology, 10(1), p. 13. (doi: 10.1186/1471-2121-10-13)
2008
Heath, R. J. W. and Insall, R. H. (2008) Dictyostelium MEGAPs: F-BAR domain proteins that regulate motility and membrane tubulation in contractile vacuoles. Journal of Cell Science, 121(7), pp. 1054-1064. (doi: 10.1242/jcs.021113)
Heath, R. J. W. and Insall, R. H. (2008) F-BAR domains: multifunctional regulators of membrane curvature. Journal of Cell Science, 121(12), pp. 1951-1954. (doi: 10.1242/jcs.023895)
King, J.S. and Insall, R.H. (2008) Chemotaxis: TorC before You Akt…. Current Biology, 18(18), R864-R866. (doi: 10.1016/j.cub.2008.07.051)
Pollitt, A.Y. and Insall, R.H. (2008) Abi Mutants in Dictyostelium Reveal Specific Roles for the SCAR/WAVE Complex in Cytokinesis. Current Biology, 18(3), pp. 203-210. (doi: 10.1016/j.cub.2008.01.026)
2007
Zaki, M., King, J., Fütterer, K. and Insall, R. H. (2007) Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics. BMC Genetics, 8(1), 28. (doi: 10.1186/1471-2156-8-28) (PMID:17553170) (PMCID:PMC1904233)
Kae, H., Kortholt, A., Rehmann, H., Insall, R. H. , Van Haastert, P. J.M., Spiegelman, G. B. and Weeks, G. (2007) Cyclic AMP signalling in Dictyostelium: G-proteins activate separate Ras pathways using specific RasGEFs. EMBO Reports, 8(5), pp. 477-482. (doi: 10.1038/sj.embor.7400936) (PMID:17380187) (PMCID:PMC1866193)
Andrew, N. and Insall, R. H. (2007) Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusions. Nature Cell Biology, 9(2), pp. 193-200. (doi: 10.1038/ncb1536) (PMID:17220879)
Insall, R. and Andrew, N. (2007) Chemotaxis in Dictyostelium: how to walk straight using parallel pathways. Current Opinion in Microbiology, 10(6), pp. 578-581. (doi: 10.1016/j.mib.2007.10.004)
2006
Zaki, M., Andrew, N. and Insall, R. H. (2006) Entamoeba histolytica cell movement: a central role for self-generated chemokines and chemorepellents. Proceedings of the National Academy of Sciences of the United States of America, 103(49), pp. 18751-18756. (doi: 10.1073/pnas.0605437103) (PMID:17132728) (PMCID:PMC1693734)
Somesh, B. P., Vlahou, G., Iijima, M., Insall, R. H. , Devreotes, P. and Rivero, F. (2006) RacG regulates morphology, phagocytosis, and chemotaxis. Eukaryotic Cell, 5(10), pp. 1648-1663. (doi: 10.1128/EC.00221-06) (PMID:16950926) (PMCID:PMC1595345)
Pollitt, A. Y., Blagg, S. L., Ibarra, N. and Insall, R. H. (2006) Cell motility and SCAR localisation in axenically growing Dictyostelium cells. European Journal of Cell Biology, 85(9-10), pp. 1091-1098. (doi: 10.1016/j.ejcb.2006.05.014) (PMID:16822579)
Insall, R. H. and Jones, G. E. (2006) Moving matters: signals and mechanisms in directed cell migration. Nature Cell Biology, 8(8), pp. 776-779. (doi: 10.1038/ncb0806-776) (PMID:16880806)
Ibarra, N., Blagg, S. L., Vazquez, F. and Insall, R. H. (2006) Nap1 regulates Dictyostelium cell motility and adhesion through SCAR-dependent and -independent pathways. Current Biology, 16(7), pp. 717-722. (doi: 10.1016/j.cub.2006.02.068) (PMID:16581519)
King, J. and Insall, R. (2006) Parasexual genetics using axenic cells. Methods in Molecular Biology, 346, pp. 125-136. (doi: 10.1385/1-59745-144-4:125) (PMID:16957288)
2005
Ibarra, N., Pollitt, A. and Insall, R.H. (2005) Regulation of actin assembly by SCAR/WAVE proteins. Biochemical Society Transactions, 33(6), pp. 1243-1246. (doi: 10.1042/BST0331243) (PMID:16246088)
Wilkins, A., Szafranski, K., Fraser, D. J., Bakthavatsalam, D., Müller, R., Fisher, P. R., Glöckner, G., Eichinger, L., Noegel, A. A. and Insall, R. H. (2005) The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles. Genome Biology, 6(8), R68. (doi: 10.1186/gb-2005-6-8-r68) (PMID:16086850) (PMCID:PMC1273635)
Zouwail, S., Pettit, T. R., Dove, S. K., Chibalina, M. V., Powner, D. J., Haynes, L., Wakelam, M. J.O. and Insall, R. H. (2005) Phospholipase D activity is essential for actin localization and actin-based motility in Dictyostelium. Biochemical Journal, 389(1), pp. 207-214. (doi: 10.1042/BJ20050085) (PMID:15769249)
Insall, R. (2005) The Dictyostelium genome: the private life of a social model revealed? Genome Biology, 6(6), 222. (doi: 10.1186/gb-2005-6-6-222) (PMID:15960812) (PMCID:PMC1175964)
2004
Blagg, S.L. and Insall, R.H. (2004) Control of SCAR activity in Dictyostelium discoideum. Biochemical Society Transactions, 32(6), pp. 1113-1114. (doi: 10.1042/BST0321113) (PMID:15506982)
Insall, R. (2004) Presidential candidates failed peer-review test. Nature, 432(7016), p. 439. (doi: 10.1038/432439c) (PMID:15565125)
Morgan, C. P., Insall, R. , Haynes, L. and Cockcroft, S. (2004) Identification of phospholipase B from Dictyostelium discoideumreveals a new lipase family present in mammals, flies and nematodes, but not yeast. Biochemical Journal, 382(2), pp. 441-449. (doi: 10.1042/BJ20040110)
Secko, D. M., Insall, R. H. , Spiegelman, G. B. and Weeks, G. (2004) The identification of Dictyostelium phosphoproteins altered in response to the activation of RasG. Proteomics, 4(9), pp. 2629-2639. (doi: 10.1002/pmic.200300788) (PMID:15352238)
Chibalina, M. V., Anjard, C. and Insall, R. H. (2004) Gdt2 regulates the transition of Dictyosteliumcells from growth to differentiation. BMC Developmental Biology, 4(1), 8. (doi: 10.1186/1471-213X-4-8)
Insall, R. H. and Machesky, L. M. (2004) Regulation of WASP: PIP2 pipped By Toca-1? Cell, 118(2), pp. 140-141. (doi: 10.1016/j.cell.2004.07.005) (PMID:15260983)
Blagg, S. L. and Insall, R. H. (2004) Solving the WAVE function. Nature Cell Biology, 6(4), pp. 279-281. (doi: 10.1038/ncb0404-279) (PMID:15057236)
Insall, R. H. (2004) PI3K and chemotaxis: localized patches make the cell’s eyes... Drug Discovery Today, 9(2), p. 97. (doi: 10.1016/S1359-6446(03)02923-4)
Blagg, S. and Insall, R. (2004) Scar, WASp and the Arp2/3 complex in Dictyostelium migration. In: Ridley, A., Peckham, M. and Clark, P. (eds.) Cell Motility: From Molecules to Organisms. Wiley: Chichester, pp. 247-260. ISBN 9780470848722 (doi: 10.1002/0470011742.ch16)
2003
Blagg, S. L., Stewart, M., Sambles, C. and Insall, R. H. (2003) PIR121 regulates pseudopod dynamics and SCAR activity in Dictyostelium. Current Biology, 13(17), pp. 1480-1487. (doi: 10.1016/S0960-9822(03)00580-3) (PMID:12956949)
King, J. and Insall, R. H. (2003) Parasexual genetics of Dictyostelium gene disruptions: identification of a ras pathway using diploids. BMC Genetics, 4(1), 12. (doi: 10.1186/1471-2156-4-12) (PMID:12854977) (PMCID:PMC183827)
Insall, R. (2003) Impact factors: target the funding bodies. Nature, 423(6940), p. 585. (doi: 10.1038/423585b) (PMID:12789312)
Insall, R. (2003) Protein conformation: through a lens, darkly. Biochemical Journal, 372(1), e1-e2. (doi: 10.1042/bj20030358COM) (PMID:12939165)
Insall, R. (2003) Dictyostelium chemotaxis: fascism through the back door? Current Biology, 13(9), R353-R354. (doi: 10.1016/S0960-9822(03)00274-4) (PMID:12725750)
Insall, R. (2003) Robert Insall. Current Biology, 13(5), R167-R168. (doi: 10.1016/s0960-9822(03)00112-x) (PMID:12620201)
Anderson, S. I., Behrendt, B., Machesky, L. M. , Insall, R. H. and Nash, G. B. (2003) Linked regulation of motility and integrin function in activated migrating neutrophils revealed by interference in remodelling of the cytoskeleton. Cell Motility and the Cytoskeleton, 54(2), pp. 135-146. (doi: 10.1002/cm.10091) (PMID:12529859)
2002
Chubb, J. R., Wilkins, A., Wessels, D. J., Soll, D. R. and Insall, R. H. (2002) Pseudopodium dynamics and rapid cell movement in Dictyostelium Ras pathway mutants. Cell Motility and the Cytoskeleton, 53(2), pp. 150-162. (doi: 10.1002/cm.10064) (PMID:12211111)
Garcia, M. X. U., Roberts, C., Alexander, H., Stewart, A. M., Harwood, A., Alexander, S. and Insall, R. H. (2002) Methanol and acriflavine resistance in Dictyostelium are caused by loss of catalase. Microbiology, 148(1), pp. 333-340. (doi: 10.1099/00221287-148-1-333) (PMID:11782526)
2001
Insall, R. H. and Weiner, O. D. (2001) PIP3, PIP2, and cell movement—similar messages, different meanings? Developmental Cell, 1(6), pp. 743-747. (doi: 10.1016/S1534-5807(01)00086-7) (PMID:11740936) (PMCID:PMC2819114)
Insall, R. , Müller-Taubenberger, A., Machesky, L. , Köhler, J., Simmeth, E., Atkinson, S. J., Weber, I. and Gerisch, G. (2001) Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis. Cell Motility and the Cytoskeleton, 50(3), pp. 115-128. (doi: 10.1002/cm.10005) (PMID:11807934)
Insall, R. H. (2001) The whole organism, and nothing but the organism. Cell, 107(3), pp. 279-281. (doi: 10.1016/S0092-8674(01)00558-X)[Book Review]
Machesky, L. M. , Insall, R. H. and Volkman, L. E. (2001) WASP homology sequences in baculoviruses. Trends in Cell Biology, 11(7), pp. 286-287. (doi: 10.1016/S0962-8924(01)02009-8) (PMID:11434350)
Chubb, J. R. and Insall, R. H. (2001) Dictyostelium: an ideal organism for genetic dissection of Ras signalling networks. Biochimica et Biophysica Acta: General Subjects, 1525(3), pp. 262-271. (doi: 10.1016/S0304-4165(01)00111-8) (PMID:11257439)
Wilkins, A. and Insall, R. H. (2001) Small GTPases in Dictyostelium: lessons from a social amoeba. Trends in Genetics, 17(1), pp. 41-48. (doi: 10.1016/S0168-9525(00)02181-8) (PMID:11163921)
Insall, R. and Machesky, L. (2001) Cytoskeleton. Encyclopedia of Life Sciences, (doi: 10.1038/npg.els.0001252)
2000
Wilkins, A., Chubb, J. R. and Insall, R. H. (2000) A novel Dictyostelium RasGEF is required for normal endocytosis, cell motility and multicellular development. Current Biology, 10(22), pp. 1427-1437. (doi: 10.1016/S0960-9822(00)00797-1) (PMID:11102804)
Insall, R. (2000) The PKB that doesn’t need to jump. Trends in Genetics, 16(10), p. 441. (doi: 10.1016/S0168-9525(00)02130-2) (PMID:11050328)
Traynor, D., Milne, J. L.S., Insall, R. H. and Kay, R. R. (2000) Ca2+ signalling is not required for chemotaxis in Dictyostelium. EMBO Journal, 19(17), pp. 4846-4854. (doi: 10.1093/emboj/19.17.4846) (PMID:10970875) (PMCID:PMC302083)
Wilkins, A., Khosla, M., Fraser, D. J., Spiegelman, G. B., Fisher, P. R., Weeks, G. and Insall, R. H. (2000) Dictyostelium RasD is required for normal phototaxis, but not differentiation. Genes and Development, 14(11), pp. 1407-1413. (doi: 10.1101/gad.14.11.1407) (PMID:10837033) (PMCID:PMC316659)
Swigart, P., Insall, R. , Wilkins, A. and Cockroft, S. (2000) Purification and cloning of phosphatidylinositol transfer proteins from Dictyostelium discoideum: homologues of both mammalian PITPs and Saccharomyces cerevisiae Sec14p are found in the same cell. Biochemical Journal, 347(3), pp. 837-843. (doi: 10.1042/bj3470837) (PMID:10769190)
Insall, R. (2000) Too much of a good thing? Current Biology, 10(7), R253. (doi: 10.1016/S0960-9822(00)00409-7) (PMID:10753757)
Khosla, M., Spiegelman, G. B., Insall, R. and Weeks, G. (2000) Functional overlap of the dictyostelium RasG, RasD and RasB proteins. Journal of Cell Science, 113(8), pp. 1427-1434. (PMID:10725225)
Rozelle, A.L., Machesky, L.M. , Yamamoto, M., Driessens, M.H.E., Insall, R.H. , Roth, M.G., Luby-Phelps, K., Marriott, G., Hall, A. and Yin, H.L. (2000) Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3. Current Biology, 10(6), pp. 311-320. (doi: 10.1016/S0960-9822(00)00384-5) (PMID:10744973)
Chubb, J. R., Wilkins, A., Thomas, G. M. and Insall, R. H. (2000) The Dictyostelium RasS protein is required for macropinocytosis, phagocytosis and the control of cell movement. Journal of Cell Science, 113(4), pp. 709-719. (PMID:10652263)
1999
Loomis, W. F. and Insall, R. H. (1999) A cell for all reasons. Nature, 401(6752), pp. 440-441. (doi: 10.1038/46713) (PMID:10519543)
Lee, S., Parent, C. A., Insall, R. and Firtel, R. A. (1999) A novel Ras-interacting protein required for chemotaxis and cyclic adenosine monophosphate signal relay in Dictyostelium. Molecular Biology of the Cell, 10(9), pp. 2829-2845. (doi: 10.1091/mbc.10.9.2829) (PMID:10473630) (PMCID:PMC25521)
Machesky, L. M. and Insall, R. H. (1999) Signaling to actin dynamics. Journal of Cell Biology, 146(2), pp. 267-272. (doi: 10.1083/jcb.146.2.267) (PMID:10427083) (PMCID:PMC2156183)
Insall, R. (1999) PKB starts to jump. Trends in Genetics, 15(7), p. 260. (doi: 10.1016/S0168-9525(99)01802-8)
Insall, R. and Machesky, L. (1999) PH domains in WASP – a bug in the system? Trends in Cell Biology, 9(6), p. 211. (doi: 10.1016/S0962-8924(99)01569-X) (PMID:10354565)
Insall, R. (1999) Cynicism and credulity. Current Biology, 9(7), R231. (doi: 10.1016/S0960-9822(99)80147-X) (PMID:10209127)
1998
Machesky, L. M. and Insall, R. H. (1998) Scar1 and the related Wiskott–Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex. Current Biology, 8(25), pp. 1347-1356. (doi: 10.1016/S0960-9822(98)00015-3) (PMID:9889097)
Insall, R. (1998) Keeping it in the family. Current Biology, 8(12), R401. (doi: 10.1016/S0960-9822(98)70258-1)
Machesky, L. M. , Insall, R. H. and Kay, R. R. (1998) The helC gene encodes a putative DEAD-box RNA helicase required for development in Dictyostelium discoideum. Current Biology, 8(10), 607-S4. (doi: 10.1016/S0960-9822(98)70233-7) (PMID:9601648)
1997
Insall, R. (1997) The right stuff. Current Biology, 7(11), R665. (doi: 10.1016/S0960-9822(06)00345-9) (PMID:9382817)
Tuxworth, R. I., Cheetham, J. L., Machesky, L. M. , Spiegelmann, G. B., Weeks, G. and Insall, R. H. (1997) Dictyostelium RasG is required for normal motility and cytokinesis, but not growth. Journal of Cell Biology, 138(3), pp. 605-614. (doi: 10.1083/jcb.138.3.605) (PMID:9245789) (PMCID:PMC2141629)
Insall, R. (1997) The talk's the thing. Current Biology, 7(6), R331. (doi: 10.1016/S0960-9822(06)00161-8)
Aubry, L., Maeda, M., Insall, R. , Devreotes, P. N. and Firtel, R. A. (1997) The Dictyostelium mitogen-activated protein kinase ERK2 is regulated by Ras and cAMP-dependent protein kinase (PKA) and mediates PKA function. Journal of Biological Chemistry, 272(7), pp. 3883-3886. (doi: 10.1074/jbc.272.7.3883) (PMID:9020088)
Insall, R. (1997) The enemy within. Current Biology, 7(2), R59. (doi: 10.1016/S0960-9822(06)00032-7) (PMID:9081661)
1996
Insall, R. (1996) The art of MBoC by B.A. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts and J. Watson. Trends in Genetics, 12(10), p. 430. (doi: 10.1016/0168-9525(96)81491-0)[Book Review]
Insall, R. H. , Borleis, J. and Devreotes, P. N. (1996) The aimless RasGEF is required for processing of chemotactic signals through G-protein-coupled receptors in Dictyostelium. Current Biology, 6(6), pp. 719-729. (doi: 10.1016/S0960-9822(09)00453-9) (PMID:8793298)
Insall, R. H. (1996) Osmoregulation: cyclic GMP and the big squeeze. Current Biology, 6(5), pp. 516-518. (doi: 10.1016/S0960-9822(02)00530-4) (PMID:8805270)
Maeda, M., Aubry, L., Insall, R. , Gaskins, C., Devreotes, P. N. and Firtel, R. A. (1996) Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium. Journal of Biological Chemistry, 271(7), pp. 3351-3354. (doi: 10.1074/jbc.271.7.3351) (PMID:8631932)
Chen, M.-Y., Insall, R. H. and Devreotes, P. N. (1996) Signaling through chemoattractant receptors in Dictyostelium. Trends in Genetics, 12(2), pp. 52-57. (doi: 10.1016/0168-9525(96)81400-4) (PMID:8851971)
Insall, R. (1996) And now it's time for tea. Current Biology, 6(2), p. 99. (doi: 10.1016/S0960-9822(02)00427-X) (PMID:8859322)
1995
Insall, R. (1995) Glycogen synthase kinase and Dictyostelium development: old pathways pointing in new directions? Trends in Genetics, 11(2), pp. 37-39. (doi: 10.1016/S0168-9525(00)88989-1) (PMID:7716803)
1994
Insall, R. , Kuspa, A., Lilly, P. J., Shaulsky, G., Levin, L. R., Loomis, W. F. and Devreotes, P. (1994) CRAC, a cytosolic protein containing a pleckstrin homology domain, is required for receptor and G protein-mediated activation of adenylyl cyclase in Dictyostelium. Journal of Cell Biology, 126(6), pp. 1537-1545. (doi: 10.1083/jcb.126.6.1537) (PMID:8089184) (PMCID:PMC2290948)
Soede, R. D.M., Insall, R. H. , Devreotes, P. N. and Schaap, P. (1994) Extracellular cAMP can restore development in Dictyostelium cells lacking one, but not two subtypes of early cAMP receptors (cARs). Evidence for involvement of cAR1 in aggregative gene expression. Development, 120(7), pp. 1997-2002. (PMID:7925004)
Insall, R. H. , Soede, R. D.M., Schaap, P. and Devreotes, P. N. (1994) Two cAMP receptors activate common signaling pathways in Dictyostelium. Molecular Biology of the Cell, 5(6), pp. 703-711. (doi: 10.1091/mbc.5.6.703) (PMID:7949426) (PMCID:PMC301085)
1992
Pupillo, M., Insall, R. , Pitt, G. S. and Devreotes, P. N. (1992) Multiple cyclic AMP receptors are linked to adenylyl cyclase in Dictyostelium. Molecular Biology of the Cell, 3(11), pp. 1229-1234. (doi: 10.1091/mbc.3.11.1229) (PMID:1333842) (PMCID:PMC275689)
Insall, R. , Nayler, O. and Kay, R. R. (1992) DIF-1 induces its own breakdown in Dictyostelium. EMBO Journal, 11(8), pp. 2849-2854. (PMID:1322291) (PMCID:PMC556764)
Ceccarelli, A., Mahbubani, H. J., Insall, R. , Schnitzler, G., Firtel, R. A. and Williams, J. G. (1992) A G-rich sequence element common to Dictyostelium genes which differ radically in their patterns of expression. Developmental Biology, 152(1), pp. 188-193. (doi: 10.1016/0012-1606(92)90169-H) (PMID:1321061)
1991
Berks, M., Traynor, D., Carrin, I., Insall, R. H. and Kay, R. R. (1991) Diffusible signal molecules controlling cell differentiation and patterning in Dictyostelium. Development, 113(S1), pp. 131-139. (PMID:1660326)
1990
Insall, R. and Kay, R. R. (1990) A specific DIF binding protein in Dictyostelium. EMBO Journal, 9(10), pp. 3323-3328. (PMID:2170113) (PMCID:PMC552069)
Articles
Dowdell, A., Paschke, P. I., Thomason, P. A., Tweedy, L. and Insall, R. H. (2023) Competition between chemoattractants causes unexpected complexity and can explain negative chemotaxis. Current Biology, 32(9), 1704-1715.e3. (doi: 10.1016/j.cub.2023.03.006) (PMID:37001521)
Insall, R. (2023) Science Twitter — navigating change in science communication. Nature Reviews Molecular Cell Biology, 24, pp. 305-306. (doi: 10.1038/s41580-023-00581-3) (PMID:36732603)
Insall, R. H. (2023) Receptors, enzymes and self-attraction as autocrine generators and amplifiers of chemotaxis and cell steering. Current Opinion in Cell Biology, 81, 102169. (doi: 10.1016/j.ceb.2023.102169) (PMID:37075582)
Singh, S. P. , Paschke, P., Tweedy, L. and Insall, R. H. (2022) AKT and SGK kinases regulate cell migration by altering Scar/WAVE complex activation and Arp2/3 complex recruitment. Frontiers in Molecular Biosciences, 9, 965921. (doi: 10.3389/fmolb.2022.965921) (PMID:36106016) (PMCID:PMC9466652)
Insall, R. H. , Paschke, P. and Tweedy, L. (2022) Steering yourself by the bootstraps: how cells create their own gradients for chemotaxis. Trends in Cell Biology, 32(7), pp. 585-596. (doi: 10.1016/j.tcb.2022.02.007) (PMID:35351380)
Edgar, J. M. , McGowan, E., Chapple, K., Möbius, W., Lemgruber, L. , Insall, R. H. , Nave, K.-A. and Boullerne, A. (2021) Rio-Hortega's drawings revisited with fluorescent proteins define a cytoplasm filled channel system of CNS myelin. Journal of Anatomy, 239(6), pp. 1241-1255. (doi: 10.1111/joa.13577)
Singh, S. P. , Thomason, P. A. and Insall, R. H. (2021) Extracellular signalling modulates Scar/WAVE complex activity through Abi phosphorylation. Cells, 10(12), 3485. (doi: 10.3390/cells10123485) (PMID:34943993) (PMCID:PMC8700165)
Williams, R. S.B., Chubb, J. R., Insall, R. , King, J. S., Pears, C. J., Thompson, E. and Weijer, C. J. (2021) Moving the research forward: the best of British biology using the tractable model system Dictyostelium discoideum. Cells, 10(11), 3036. (doi: 10.3390/cells10113036) (PMID:34831258) (PMCID:PMC8616412)
Yang, Y., Li, D., Chao, X., Singh, S. P. , Thomason, P., Yan, Y., Dong, M., Li, L., Insall, R. H. and Cai, H. (2021) Leep1 interacts with PIP3 and the Scar/WAVE complex to regulate cell migration and macropinocytosis. Journal of Cell Biology, 220(7), e202010096. (doi: 10.1083/jcb.202010096) (PMID:33978708) (PMCID:PMC8127007)
Yelland, T., Le, A. H., Nikolaou, S., Insall, R. , Machesky, L. and Ismail, S. (2021) Structural basis of CYRI-B direct competition with Scar/WAVE complex for Rac1. Structure, 29(3), 226-237.e4. (doi: 10.1016/j.str.2020.11.003) (PMID:33217330) (PMCID:PMC7955166)
Singh, S. P. and Insall, R. H. (2021) Adhesion stimulates Scar/WAVE phosphorylation in mammalian cells. Communicative and Integrative Biology, 14(1), pp. 1-4. (doi: 10.1080/19420889.2020.1855854) (PMID:33447346) (PMCID:PMC7781634)
Zickus, V. et al. (2020) Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation. Scientific Reports, 10, 20986. (doi: 10.1038/s41598-020-77737-0) (PMID:33268900) (PMCID:PMC7710711)
Tweedy, L., Thomason, P. A., Paschke, P. I., Martin, K., Machesky, L. M. , Zagnoni, M. and Insall, R. H. (2020) Seeing around corners: cells solve mazes and respond at a distance using attractant breakdown. Science, 369(6507), eaay9792. (doi: 10.1126/science.aay9792) (PMID:32855311)
Singh, S. P. , Thomason, P. A., Lilla, S., Schaks, M., Tang, Q., Goode, B. L., Machesky, L. M. , Rottner, K. and Insall, R. H. (2020) Cell–substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime. PLoS Biology, 18(8), e3000774. (doi: 10.1371/journal.pbio.3000774) (PMID:32745097) (PMCID:PMC7425996)
McMenamin, P. G., Shields, G. T., Seyed-Razavi, Y., Kalirai, H., Insall, R. H. , Machesky, L. M. and Coupland, S. E. (2020) Melanoblasts populate the mouse choroid earlier in development than previously described. Investigative Ophthalmology and Visual Science, 61(10), 33. (doi: 10.1167/iovs.61.10.33) (PMID:32797202) (PMCID:PMC7441366)
Kadir, S. R., Insall, R. H. , Moffatt, G., McGhee, J. and Livingstone, D. (2020) Analogies in 3D molecular visualisations: development of a cell biology animation ‘How cells move – a new interpretation of old data’. Journal of Visual Communication in Medicine, 43(1), pp. 35-46. (doi: 10.1080/17453054.2019.1671814) (PMID:31642358)
Amato, C., Thomason, P. A., Davidson, A. J., Swaminathan, K., Ismail, S. , Machesky, L. M. and Insall, R. H. (2019) WASP restricts active Rac to maintain cells’ front-rear polarization. Current Biology, 29(24), pp. 4169-4182. (doi: 10.1016/j.cub.2019.10.036) (PMID:31786060) (PMCID:PMC6926487)
Juin, A. et al. (2019) N-WASP control of LPAR1 trafficking establishes response to self-generated LPA gradients to promote pancreatic cancer cell metastasis. Developmental Cell, 51(4), 431-445.e7. (doi: 10.1016/j.devcel.2019.09.018) (PMID:31668663) (PMCID:PMC6863394)
Tweedy, L., Witzel, P., Heinrich, D., Insall, R. H. and Endres, R. G. (2019) Screening by changes in stereotypical behavior during cell motility. Scientific Reports, 9, 8784. (doi: 10.1038/s41598-019-45305-w) (PMID:31217532) (PMCID:PMC6584642)
Buckley, C. M. et al. (2019) PIKfyve/Fab1 is required for efficient V-ATPase and hydrolase delivery to phagosomes, phagosomal killing, and restriction of Legionella infection. PLoS Pathogens, 15(2), e1007551. (doi: 10.1371/journal.ppat.1007551) (PMID:30730983) (PMCID:PMC6382210)
Schaks, M., Singh, S. P. , Kage, F., Thomason, P., Klünemann, T., Steffen, A., Blankenfeldt, W., Stradal, T. E., Insall, R. H. and Rottner, K. (2018) Distinct interaction sites of Rac GTPase with WAVE regulatory complex have non-redundant functions in vivo. Current Biology, 28(22), 3674-3684.e6. (doi: 10.1016/j.cub.2018.10.002) (PMID:30393033) (PMCID:PMC6264382)
Fort, L. et al. (2018) Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nature Cell Biology, 20(10), pp. 1159-1171. (doi: 10.1038/s41556-018-0198-9) (PMID:30250061) (PMCID:PMC6863750)
Visweshwaran, S. P. et al. (2018) The trimeric coiled‐coil HSBP 1 protein promotes WASH complex assembly at centrosomes. EMBO Journal, 37(13), e97706. (doi: 10.15252/embj.201797706) (PMID:29844016) (PMCID:PMC6028030)
Paschke, P., Knecht, D. A., Silale, A., Traynor, D., Williams, T. D., Thomason, P. A., Insall, R. H. , Chubb, J. R., Kay, R. R. and Veltman, D. M. (2018) Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum. PLoS ONE, 13(5), e0196809. (doi: 10.1371/journal.pone.0196809) (PMID:29847546) (PMCID:PMC5976153)
Pan, M., Neilson, M. P., Grunfeld, A. M., Cruz, P., Wen, X., Insall, R. H. and Jin, T. (2018) A G-protein-coupled chemoattractant receptor recognizes lipopolysaccharide for bacterial phagocytosis. PLoS Biology, 16(5), e2005754. (doi: 10.1371/journal.pbio.2005754) (PMID:29799847) (PMCID:PMC5969738)
Davidson, A. J., Amato, C., Thomason, P. A. and Insall, R. H. (2018) WASP family proteins and formins compete in pseudopod- and bleb-based migration. Journal of Cell Biology, 217(2), pp. 701-714. (doi: 10.1083/jcb.201705160) (PMID:29191847) (PMCID:PMC5800805)
Susanto, O. et al. (2017) LPP3 mediates self-generation of chemotactic LPA gradients by melanoma cells. Journal of Cell Science, 130(20), pp. 3455-3466. (doi: 10.1242/jcs.207514) (PMID:28871044) (PMCID:PMC5665449)
Ferguson, E. A. , Matthiopoulos, J. , Insall, R. H. and Husmeier, D. (2017) Statistical inference of the mechanisms driving collective cell movement. Journal of the Royal Statistical Society: Series C (Applied Statistics), 66(4), pp. 869-890. (doi: 10.1111/rssc.12203)
Thomason, P. A., King, J. S. and Insall, R. H. (2017) Mroh1, a lysosomal regulator localized by WASH-generated actin. Journal of Cell Science, 130(10), pp. 1785-1795. (doi: 10.1242/jcs.197210) (PMID:28424231) (PMCID:PMC5450189)
Periz, J. et al. (2017) Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation. eLife, 6, e24119. (doi: 10.7554/eLife.24119) (PMID:28322189) (PMCID:PMC5375643)
Woodham, E. F. et al. (2017) Coordination by Cdc42 of actin, contractility, and adhesion for melanoblast movement in mouse skin. Current Biology, 27(5), pp. 624-637. (doi: 10.1016/j.cub.2017.01.033) (PMID:28238662) (PMCID:PMC5344686)
Veltman, D. M., Williams, T. D., Bloomfield, G., Chen, B.-C., Betzig, E., Insall, R. and Kay, R. R. (2016) A plasma membrane template for macropinocytic cups. eLife, 5, e20085. (doi: 10.7554/eLife.20085) (PMID:27960076) (PMCID:PMC5154761)
Mackenzie, J.A., Nolan, M. and Insall, R.H. (2016) Local modulation of chemoattractant concentrations by single cells: dissection using a bulk-surface computational model. Interface Focus, 6(5), 20160036. (doi: 10.1098/rsfs.2016.0036) (PMID:27708760) (PMCID:PMC4992739)
Buckley, C. M., Gopaldass, N., Bosmani, C., Johnston, S. A., Soldati, T., Insall, R. and King, J. S. (2016) WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors. Proceedings of the National Academy of Sciences of the United States of America, 113(40), E5906-E5915. (doi: 10.1073/pnas.1524532113) (PMID:27647881) (PMCID:PMC5056073)
Ferguson, E. A. , Matthiopoulos, J. , Insall, R. H. and Husmeier, D. (2016) Inference of the drivers of collective movement in two cell types: Dictyostelium and melanoma. Journal of the Royal Society: Interface, 13(123), 20160695. (doi: 10.1098/rsif.2016.0695) (PMID:27798280)
Tweedy, L., Susanto, O. and Insall, R. H. (2016) Self-generated chemotactic gradients—cells steering themselves. Current Opinion in Cell Biology, 42, pp. 46-51. (doi: 10.1016/j.ceb.2016.04.003) (PMID:27105308)
Tyrrell, B. J., Woodham, E. F., Spence, H. J., Strathdee, D., Insall, R. H. and Machesky, L. M. (2016) Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell and Melanoma Research, 29(5), pp. 559-571. (doi: 10.1111/pcmr.12506) (PMID:27390154) (PMCID:PMC5082549)
Frej, A. D. et al. (2016) The inositol-3-phosphate synthase biosynthetic enzyme has distinct catalytic and metabolic roles. Molecular and Cellular Biology, 36(10), pp. 1464-1479. (doi: 10.1128/mcb.00039-16) (PMID:26951199) (PMCID:PMC4859692)
Tweedy, L., Knecht, D. A., Mackay, G. N. and Insall, R. H. (2016) Self-generated chemoattractant gradients: attractant depletion extends the range and robustness of chemotaxis. PLoS Biology, 14(3), e1002404. (doi: 10.1371/journal.pbio.1002404) (PMID:26981861) (PMCID:PMC4794234)
MacDonald, G., Mackenzie, J.A., Nolan, M. and Insall, R.H. (2016) A computational method for the coupled solution of reaction–diffusion equations on evolving domains and manifolds: application to a model of cell migration and chemotaxis. Journal of Computational Physics, 309, pp. 207-226. (doi: 10.1016/j.jcp.2015.12.038) (PMID:27330221) (PMCID:PMC4896117)
Siebert, S. , Machesky, L. M. and Insall, R. H. (2015) Overflow in science and its implications for trust. eLife, 4, e10825. (doi: 10.7554/eLife.10825)
Muinonen-Martin, A. J. and Insall, R. H. (2015) Melanomas’ fatal attraction to lysophosphatidic acid trails: a new prognostic and therapeutic approach? Melanoma Management, 2(2), pp. 97-100. (doi: 10.2217/mmt.15.11)
Roberts, H. M., Ling, M. R., Insall, R. , Kalna, G., Spengler, J., Grant, M. M. and Chapple, I. L.C. (2015) Impaired neutrophil directional chemotactic accuracy in chronic periodontitis patients. Journal of Clinical Periodontology, 42(1), pp. 1-11. (doi: 10.1111/jcpe.12326) (PMID:25360483) (PMCID:PMC4340045)
Urushihara, H. et al. (2015) Comparative genome and transcriptome analyses of the social amoeba Acytostelium subglobosum that accomplishes multicellular development without germ-soma differentiation. BMC Genomics, 16, 80. (doi: 10.1186/s12864-015-1278-x) (PMID:25758444) (PMCID:PMC4334915)
Muinonen-Martin, A. J. et al. (2014) Melanoma cells break down LPA to establish local gradients that drive chemotactic dispersal. PLoS Biology, 12(10), e1001966. (doi: 10.1371/journal.pbio.1001966) (PMID:25313567) (PMCID:PMC4196730)
Veltman, D.M., Lemieux, M.G., Knecht, D.A. and Insall, R.H. (2014) PIP3-dependent macropinocytosis is incompatible with chemotaxis. Journal of Cell Biology, 204(4), pp. 497-505. (doi: 10.1083/jcb.201309081)
Sapey, E., Greenwood, H., Walton, G., Mann, E., Love, A., Aaronson, N., Insall, R.H. , Stockley, R.A. and Lord, J.M. (2014) Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence. Blood, 123(2), pp. 239-248. (doi: 10.1182/blood-2013-08-519520)
Tyrrell, B. J., Neilson, M., Insall, R. H. and Machesky, L. M. (2014) Predicting cell shapes in melanomas. Pigment Cell and Melanoma Research, 27(1), pp. 5-6. (doi: 10.1111/pcmr.12176) (PMID:24118871)
Davidson, A.J., Ura, S., Thomason, P.A., Kalna, G. and Insall, R.H. (2013) Abi is required for modulation and stability but not localization or activation of the SCAR/WAVE complex. Eukaryotic Cell, 12(11), pp. 1509-1516. (doi: 10.1128/EC.00116-13) (PMID:24036345) (PMCID:PMC3837927)
Insall, R.H. (2013) WASH complex conservation: don’t WASH away the family! Trends in Cell Biology, 23(11), pp. 519-520. (doi: 10.1016/j.tcb.2013.07.009)
Insall, R. (2013) The interaction between pseudopods and extracellular signalling during chemotaxis and directed migration. Current Opinion in Cell Biology, 25(5), pp. 526-531. (doi: 10.1016/j.ceb.2013.04.009)
King, J.S., Gueho, A., Hagedorn, M., Gopaldass, N., Leuba, F., Soldati, T. and Insall, R.H. (2013) WASH is required for lysosomal recycling and efficient autophagic and phagocytic digestion. Molecular Biology of the Cell, 24(17), pp. 2714-2726. (doi: 10.1091/mbc.E13-02-0092)
Davidson, A.J., King, J.S. and Insall, R.H. (2013) The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum. BioTechniques, 55(1), pp. 39-41. (doi: 10.2144/000114054)
Park, L., Thomason, P.A., Zech, T., King, J.S., Veltman, D.M., Carnell, M., Ura, S., Machesky, L.M. and Insall, R.H. (2013) Cyclical Action of the WASH complex: FAM21 and capping protein drive WASH recycling, not initial recruitment. Developmental Cell, 24(2), pp. 169-181. (doi: 10.1016/j.devcel.2012.12.014)
Choi, C.-H., Thomason, P.A., Zaki, M., Insall, R.H. and Barber, D.D.L. (2013) Phosphorylation of actin-related protein 2 (Arp2) is required for normal development and cAMP chemotaxis in dictyostelium. Journal of Biological Chemistry, 288(4), pp. 2464-2474. (doi: 10.1074/jbc.M112.435313)
Tang, H. et al. (2013) Loss of scar/WAVE complex promotes N-WASP- and FAK-dependent invasion. Current Biology, 23(2), pp. 107-117. (doi: 10.1016/j.cub.2012.11.059)
Davidson, A. J. and Insall, R. H. (2013) SCAR/WAVE. Communicative and Integrative Biology, 6(6), e27033. (doi: 10.4161/cib.27033) (PMID:24753786) (PMCID:PMC3984289)
Itakura, A., Aslan, J.E., Kusanto, B.T., Phillips, K.G., Porter, J.E., Newton, P.K., Nan, X., Insall, R.H. , Chernoff, J. and McCarty, O.J.T. (2013) p21-activated kinase (PAK) regulates cytoskeletal reorganization and directional migration in human neutrophils. PLoS ONE, 8(9), e73063. (doi: 10.1371/journal.pone.0073063) (PMID:24019894) (PMCID:PMC3760889)
Muinonen-Martin, A.J., Knecht, D.A., Veltman, D.M., Thomason, P.A., Kalna, G. and Insall, R.H. (2013) Measuring chemotaxis using direct visualization microscope chambers. Methods in Molecular Biology, 1046, pp. 307-321. (doi: 10.1007/978-1-62703-538-5_18)
Shirazi, M. H., Marsh, P., Keane, M. G., Khushal, A. and Insall, R. (2012) Laparoscopic total extra-peritoneal inguinal hernia repairs by a surgeon experienced at laparoscopic cholecystectomy. Journal of the Pakistan Medical Association, 62(12), pp. 1301-1304. (PMID:23866478)
Yu, X. et al. (2012) N-WASP coordinates the delivery and F-actin-mediated capture of MT1-MMP at invasive pseudopods. Journal of Cell Biology, 199(3), pp. 527-544. (doi: 10.1083/jcb.201203025)
Veltman, D. M., King, J. S., Machesky, L. M. and Insall, R. H. (2012) SCAR knockouts in Dictyostelium: WASP assumes SCAR’s position and upstream regulators in pseudopods. Journal of Cell Biology, 198(4), pp. 501-508. (doi: 10.1083/jcb.201205058) (PMID:22891261) (PMCID:PMC3514037)
Pakes, N.K., Veltman, D.M., Rivero, F., Nasir, J., Insall, R. and Williams, R.S.B. (2012) The Rac GEF ZizB regulates development, cell motility and cytokinesis in Dictyostelium. Journal of Cell Science, 125(10), pp. 2457-2465. (doi: 10.1242/jcs.100966)
Ura, S., Pollitt, A.Y., Veltman, D.M., Morrice, N.A., Machesky, L.M. and Insall, R.H. (2012) Pseudopod growth and evolution during cell movement is controlled through SCAR/WAVE dephosphorylation. Current Biology, 22(7), pp. 553-561. (doi: 10.1016/j.cub.2012.02.020)
Insall, R. (2012) How a paper on RAC set the standard. Nature Reviews Molecular Cell Biology, 13(2), p. 66. (doi: 10.1038/nrm3277)
Spence, H.J., Timpson, P., Tang, H.R., Insall, R.H. and Machesky, L.M. (2012) Scar/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions. Biochemical Journal, 448(1), pp. 35-42. (doi: 10.1042/BJ20112206)
King, J.S., Veltman, D.M. and Insall, R.H. (2011) The induction of autophagy by mechanical stress. Autophagy, 7(12), pp. 1490-1499. (doi: 10.4161/auto.7.12.17924)
Li, A. et al. (2011) Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression. Developmental Cell, 21(4), pp. 722-734. (doi: 10.1016/j.devcel.2011.07.008)
Neilson, M. P., Veltman, D. M., van Haastert, P. J. M., Webb, S. D., Mackenzie, J. A. and Insall, R. H. (2011) Chemotaxis: a feedback-based computational model robustly predicts multiple aspects of real cell behaviour. PLoS Biology, 9(5), e1000618. (doi: 10.1371/journal.pbio.1000618)
Sapey, E., Stockley, J. A., Greenwood, H., Ahmad, A., Bayley, D., Lord, J. M., Insall, R. H. and Stockley, R. A. (2011) Behavioral and structural differences in migrating peripheral neutrophils from patients with chronic obstructive pulmonary disease. American Journal of Respiratory and Critical Care Medicine, 183(9), pp. 1176-1186. (doi: 10.1164/rccm.201008-1285OC)
Shirazi, M. H., Tennant, W. G. and Insall, R. (2011) Novel carotid surgery in an asymptomatic totally occluded common carotid artery. Journal of the Pakistan Medical Association, 61(2), pp. 187-189. (PMID:21375176)
Carnell, M., Zech, T., Calaminus, S.D., Ura, S., Hagedorn, M., Johnston, S.A., May, R.C., Soldati, T., Machesky, L.M. and Insall, R.H. (2011) Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis. Journal of Cell Biology, 193(5), pp. 831-839. (doi: 10.1083/jcb.201009119)
Carnell, M.J. and Insall, R.H. (2011) Actin on disease – Studying the pathobiology of cell motility using Dictyostelium discoideum. Seminars in Cell and Developmental Biology, 22(1), pp. 82-88. (doi: 10.1016/j.semcdb.2010.12.003)
Davidson, A. and Insall, R.H. (2011) Actin-Based Motility: WAVE Regulatory Complex Structure Reopens Old SCARs. Current Biology, 21(2), R66-R68. (doi: 10.1016/j.cub.2010.12.001)
Insall, R. (2011) Career postdocs increase scrap heap. Nature, 471(7340), p. 578. (doi: 10.1038/471578e)
Insall, R.H. (2011) Dogma bites back – the evidence for branched actin. Trends in Cell Biology, 21(1), p. 2. (doi: 10.1016/j.tcb.2010.11.002)
Itakura, A., Verbout, N. G., Phillips, K. G., Insall, R.H. , Gailani, D., Tucker, E. I., Gruber, A. and McCarty, O. J. T. (2011) Activated factor XI inhibits chemotaxis of polymorphonuclear leukocytes. Journal of Leukocyte Biology, 90(5), pp. 923-927. (doi: 10.1189/jlb.0411182)
Neilson, M.P., Mackenzie, J.A., Webb, S.D. and Insall, R.H. (2011) Modeling Cell Movement and Chemotaxis Using Pseudopod-Based Feedback. SIAM Journal on Scientific Computing, 33(3), pp. 1035-1057. (doi: 10.1137/100788938)
Veltman, D.W., Auciello, G., Spence, H.J., Machesky, L.M. , Rappoport, J.Z. and Insall, R.H. (2011) Functional analysis of Dictyostelium IBARa reveals a conserved role of the I-BAR domain in endocytosis. Biochemical Journal, 436(1), pp. 45-52. (doi: 10.1042/BJ20101684)
Zech, T., Calaminus, S.D.J., Caswell, P., Spence, H.J., Carnell, M., Insall, R.H. , Norman, J. and Machesky, L.M. (2011) The Arp2/3 activator WASH regulates 5 1-integrin-mediated invasive migration. Journal of Cell Science, 124(22), pp. 3753-3759. (doi: 10.1242/jcs.080986)
Neilson, M. P., Mackenzie, J. A., Webb, S. D. and Insall, R. H. (2010) Use of the parameterised finite element method to robustly and efficiently evolve the edge of a moving cell. Integrative Biology, 2(11-12), pp. 687-695. (doi: 10.1039/c0ib00047g)
Insall, R. H. (2010) Understanding eukaryotic chemotaxis: a pseudopod-centred view. Nature Reviews Molecular Cell Biology, 11(6), pp. 453-458. (doi: 10.1038/nrm2905)
Gullberg, D., Muinonen-Martin, A.J., Veltman, D.M., Kalna, G. and Insall, R. (2010) An improved chamber for direct visualisation of chemotaxis. PLoS ONE, 5(12), e15309. (doi: 10.1371/journal.pone.0015309)
King, J. S., Veltman, D. M., Georgiou, M., Baum, B. and Insall, R. H. (2010) SCAR/WAVE is activated at mitosis and drives myosin-independent cytokinesis. Journal of Cell Science, 123(13), pp. 2246-2255. (doi: 10.1242/jcs.063735)
Veltman, D.M. and Insall, R. H. (2010) WASP Family Proteins: Their Evolution and Its Physiological Implications. Molecular Biology of the Cell, 21(16), pp. 2880-2893. (doi: 10.1091/mbc.E10-04-0372)
Insall, R. and Machesky, L.M. (2009) Actin Dynamics at the Leading Edge: From Simple Machinery to Complex Networks. Developmental Cell, 17(3), pp. 310-322. (doi: 10.1016/j.devcel.2009.08.012)
King, J.S. and Insall, R.H. (2009) Chemotaxis: finding the way forward with Dictyostelium. Trends in Cell Biology, 19(10), pp. 523-530. (doi: 10.1016/j.tcb.2009.07.004)
Pollitt, A. Y. and Insall, R. H. (2009) WASP and SCAR/WAVE proteins: the drivers of actin assembly. Journal of Cell Science, 122(15), pp. 2575-2578. (doi: 10.1242/jcs.023879)
Pollitt, A.Y. and Insall, R.H. (2009) Loss of Dictyostelium HSPC300 causes a scar-like phenotype and loss of SCAR protein. BMC Cell Biology, 10(1), p. 13. (doi: 10.1186/1471-2121-10-13)
Heath, R. J. W. and Insall, R. H. (2008) Dictyostelium MEGAPs: F-BAR domain proteins that regulate motility and membrane tubulation in contractile vacuoles. Journal of Cell Science, 121(7), pp. 1054-1064. (doi: 10.1242/jcs.021113)
Heath, R. J. W. and Insall, R. H. (2008) F-BAR domains: multifunctional regulators of membrane curvature. Journal of Cell Science, 121(12), pp. 1951-1954. (doi: 10.1242/jcs.023895)
King, J.S. and Insall, R.H. (2008) Chemotaxis: TorC before You Akt…. Current Biology, 18(18), R864-R866. (doi: 10.1016/j.cub.2008.07.051)
Pollitt, A.Y. and Insall, R.H. (2008) Abi Mutants in Dictyostelium Reveal Specific Roles for the SCAR/WAVE Complex in Cytokinesis. Current Biology, 18(3), pp. 203-210. (doi: 10.1016/j.cub.2008.01.026)
Zaki, M., King, J., Fütterer, K. and Insall, R. H. (2007) Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics. BMC Genetics, 8(1), 28. (doi: 10.1186/1471-2156-8-28) (PMID:17553170) (PMCID:PMC1904233)
Kae, H., Kortholt, A., Rehmann, H., Insall, R. H. , Van Haastert, P. J.M., Spiegelman, G. B. and Weeks, G. (2007) Cyclic AMP signalling in Dictyostelium: G-proteins activate separate Ras pathways using specific RasGEFs. EMBO Reports, 8(5), pp. 477-482. (doi: 10.1038/sj.embor.7400936) (PMID:17380187) (PMCID:PMC1866193)
Andrew, N. and Insall, R. H. (2007) Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusions. Nature Cell Biology, 9(2), pp. 193-200. (doi: 10.1038/ncb1536) (PMID:17220879)
Insall, R. and Andrew, N. (2007) Chemotaxis in Dictyostelium: how to walk straight using parallel pathways. Current Opinion in Microbiology, 10(6), pp. 578-581. (doi: 10.1016/j.mib.2007.10.004)
Zaki, M., Andrew, N. and Insall, R. H. (2006) Entamoeba histolytica cell movement: a central role for self-generated chemokines and chemorepellents. Proceedings of the National Academy of Sciences of the United States of America, 103(49), pp. 18751-18756. (doi: 10.1073/pnas.0605437103) (PMID:17132728) (PMCID:PMC1693734)
Somesh, B. P., Vlahou, G., Iijima, M., Insall, R. H. , Devreotes, P. and Rivero, F. (2006) RacG regulates morphology, phagocytosis, and chemotaxis. Eukaryotic Cell, 5(10), pp. 1648-1663. (doi: 10.1128/EC.00221-06) (PMID:16950926) (PMCID:PMC1595345)
Pollitt, A. Y., Blagg, S. L., Ibarra, N. and Insall, R. H. (2006) Cell motility and SCAR localisation in axenically growing Dictyostelium cells. European Journal of Cell Biology, 85(9-10), pp. 1091-1098. (doi: 10.1016/j.ejcb.2006.05.014) (PMID:16822579)
Insall, R. H. and Jones, G. E. (2006) Moving matters: signals and mechanisms in directed cell migration. Nature Cell Biology, 8(8), pp. 776-779. (doi: 10.1038/ncb0806-776) (PMID:16880806)
Ibarra, N., Blagg, S. L., Vazquez, F. and Insall, R. H. (2006) Nap1 regulates Dictyostelium cell motility and adhesion through SCAR-dependent and -independent pathways. Current Biology, 16(7), pp. 717-722. (doi: 10.1016/j.cub.2006.02.068) (PMID:16581519)
King, J. and Insall, R. (2006) Parasexual genetics using axenic cells. Methods in Molecular Biology, 346, pp. 125-136. (doi: 10.1385/1-59745-144-4:125) (PMID:16957288)
Ibarra, N., Pollitt, A. and Insall, R.H. (2005) Regulation of actin assembly by SCAR/WAVE proteins. Biochemical Society Transactions, 33(6), pp. 1243-1246. (doi: 10.1042/BST0331243) (PMID:16246088)
Wilkins, A., Szafranski, K., Fraser, D. J., Bakthavatsalam, D., Müller, R., Fisher, P. R., Glöckner, G., Eichinger, L., Noegel, A. A. and Insall, R. H. (2005) The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles. Genome Biology, 6(8), R68. (doi: 10.1186/gb-2005-6-8-r68) (PMID:16086850) (PMCID:PMC1273635)
Zouwail, S., Pettit, T. R., Dove, S. K., Chibalina, M. V., Powner, D. J., Haynes, L., Wakelam, M. J.O. and Insall, R. H. (2005) Phospholipase D activity is essential for actin localization and actin-based motility in Dictyostelium. Biochemical Journal, 389(1), pp. 207-214. (doi: 10.1042/BJ20050085) (PMID:15769249)
Insall, R. (2005) The Dictyostelium genome: the private life of a social model revealed? Genome Biology, 6(6), 222. (doi: 10.1186/gb-2005-6-6-222) (PMID:15960812) (PMCID:PMC1175964)
Blagg, S.L. and Insall, R.H. (2004) Control of SCAR activity in Dictyostelium discoideum. Biochemical Society Transactions, 32(6), pp. 1113-1114. (doi: 10.1042/BST0321113) (PMID:15506982)
Insall, R. (2004) Presidential candidates failed peer-review test. Nature, 432(7016), p. 439. (doi: 10.1038/432439c) (PMID:15565125)
Morgan, C. P., Insall, R. , Haynes, L. and Cockcroft, S. (2004) Identification of phospholipase B from Dictyostelium discoideumreveals a new lipase family present in mammals, flies and nematodes, but not yeast. Biochemical Journal, 382(2), pp. 441-449. (doi: 10.1042/BJ20040110)
Secko, D. M., Insall, R. H. , Spiegelman, G. B. and Weeks, G. (2004) The identification of Dictyostelium phosphoproteins altered in response to the activation of RasG. Proteomics, 4(9), pp. 2629-2639. (doi: 10.1002/pmic.200300788) (PMID:15352238)
Chibalina, M. V., Anjard, C. and Insall, R. H. (2004) Gdt2 regulates the transition of Dictyosteliumcells from growth to differentiation. BMC Developmental Biology, 4(1), 8. (doi: 10.1186/1471-213X-4-8)
Insall, R. H. and Machesky, L. M. (2004) Regulation of WASP: PIP2 pipped By Toca-1? Cell, 118(2), pp. 140-141. (doi: 10.1016/j.cell.2004.07.005) (PMID:15260983)
Blagg, S. L. and Insall, R. H. (2004) Solving the WAVE function. Nature Cell Biology, 6(4), pp. 279-281. (doi: 10.1038/ncb0404-279) (PMID:15057236)
Insall, R. H. (2004) PI3K and chemotaxis: localized patches make the cell’s eyes... Drug Discovery Today, 9(2), p. 97. (doi: 10.1016/S1359-6446(03)02923-4)
Blagg, S. L., Stewart, M., Sambles, C. and Insall, R. H. (2003) PIR121 regulates pseudopod dynamics and SCAR activity in Dictyostelium. Current Biology, 13(17), pp. 1480-1487. (doi: 10.1016/S0960-9822(03)00580-3) (PMID:12956949)
King, J. and Insall, R. H. (2003) Parasexual genetics of Dictyostelium gene disruptions: identification of a ras pathway using diploids. BMC Genetics, 4(1), 12. (doi: 10.1186/1471-2156-4-12) (PMID:12854977) (PMCID:PMC183827)
Insall, R. (2003) Impact factors: target the funding bodies. Nature, 423(6940), p. 585. (doi: 10.1038/423585b) (PMID:12789312)
Insall, R. (2003) Protein conformation: through a lens, darkly. Biochemical Journal, 372(1), e1-e2. (doi: 10.1042/bj20030358COM) (PMID:12939165)
Insall, R. (2003) Dictyostelium chemotaxis: fascism through the back door? Current Biology, 13(9), R353-R354. (doi: 10.1016/S0960-9822(03)00274-4) (PMID:12725750)
Insall, R. (2003) Robert Insall. Current Biology, 13(5), R167-R168. (doi: 10.1016/s0960-9822(03)00112-x) (PMID:12620201)
Anderson, S. I., Behrendt, B., Machesky, L. M. , Insall, R. H. and Nash, G. B. (2003) Linked regulation of motility and integrin function in activated migrating neutrophils revealed by interference in remodelling of the cytoskeleton. Cell Motility and the Cytoskeleton, 54(2), pp. 135-146. (doi: 10.1002/cm.10091) (PMID:12529859)
Chubb, J. R., Wilkins, A., Wessels, D. J., Soll, D. R. and Insall, R. H. (2002) Pseudopodium dynamics and rapid cell movement in Dictyostelium Ras pathway mutants. Cell Motility and the Cytoskeleton, 53(2), pp. 150-162. (doi: 10.1002/cm.10064) (PMID:12211111)
Garcia, M. X. U., Roberts, C., Alexander, H., Stewart, A. M., Harwood, A., Alexander, S. and Insall, R. H. (2002) Methanol and acriflavine resistance in Dictyostelium are caused by loss of catalase. Microbiology, 148(1), pp. 333-340. (doi: 10.1099/00221287-148-1-333) (PMID:11782526)
Insall, R. H. and Weiner, O. D. (2001) PIP3, PIP2, and cell movement—similar messages, different meanings? Developmental Cell, 1(6), pp. 743-747. (doi: 10.1016/S1534-5807(01)00086-7) (PMID:11740936) (PMCID:PMC2819114)
Insall, R. , Müller-Taubenberger, A., Machesky, L. , Köhler, J., Simmeth, E., Atkinson, S. J., Weber, I. and Gerisch, G. (2001) Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis. Cell Motility and the Cytoskeleton, 50(3), pp. 115-128. (doi: 10.1002/cm.10005) (PMID:11807934)
Machesky, L. M. , Insall, R. H. and Volkman, L. E. (2001) WASP homology sequences in baculoviruses. Trends in Cell Biology, 11(7), pp. 286-287. (doi: 10.1016/S0962-8924(01)02009-8) (PMID:11434350)
Chubb, J. R. and Insall, R. H. (2001) Dictyostelium: an ideal organism for genetic dissection of Ras signalling networks. Biochimica et Biophysica Acta: General Subjects, 1525(3), pp. 262-271. (doi: 10.1016/S0304-4165(01)00111-8) (PMID:11257439)
Wilkins, A. and Insall, R. H. (2001) Small GTPases in Dictyostelium: lessons from a social amoeba. Trends in Genetics, 17(1), pp. 41-48. (doi: 10.1016/S0168-9525(00)02181-8) (PMID:11163921)
Insall, R. and Machesky, L. (2001) Cytoskeleton. Encyclopedia of Life Sciences, (doi: 10.1038/npg.els.0001252)
Wilkins, A., Chubb, J. R. and Insall, R. H. (2000) A novel Dictyostelium RasGEF is required for normal endocytosis, cell motility and multicellular development. Current Biology, 10(22), pp. 1427-1437. (doi: 10.1016/S0960-9822(00)00797-1) (PMID:11102804)
Insall, R. (2000) The PKB that doesn’t need to jump. Trends in Genetics, 16(10), p. 441. (doi: 10.1016/S0168-9525(00)02130-2) (PMID:11050328)
Traynor, D., Milne, J. L.S., Insall, R. H. and Kay, R. R. (2000) Ca2+ signalling is not required for chemotaxis in Dictyostelium. EMBO Journal, 19(17), pp. 4846-4854. (doi: 10.1093/emboj/19.17.4846) (PMID:10970875) (PMCID:PMC302083)
Wilkins, A., Khosla, M., Fraser, D. J., Spiegelman, G. B., Fisher, P. R., Weeks, G. and Insall, R. H. (2000) Dictyostelium RasD is required for normal phototaxis, but not differentiation. Genes and Development, 14(11), pp. 1407-1413. (doi: 10.1101/gad.14.11.1407) (PMID:10837033) (PMCID:PMC316659)
Swigart, P., Insall, R. , Wilkins, A. and Cockroft, S. (2000) Purification and cloning of phosphatidylinositol transfer proteins from Dictyostelium discoideum: homologues of both mammalian PITPs and Saccharomyces cerevisiae Sec14p are found in the same cell. Biochemical Journal, 347(3), pp. 837-843. (doi: 10.1042/bj3470837) (PMID:10769190)
Insall, R. (2000) Too much of a good thing? Current Biology, 10(7), R253. (doi: 10.1016/S0960-9822(00)00409-7) (PMID:10753757)
Khosla, M., Spiegelman, G. B., Insall, R. and Weeks, G. (2000) Functional overlap of the dictyostelium RasG, RasD and RasB proteins. Journal of Cell Science, 113(8), pp. 1427-1434. (PMID:10725225)
Rozelle, A.L., Machesky, L.M. , Yamamoto, M., Driessens, M.H.E., Insall, R.H. , Roth, M.G., Luby-Phelps, K., Marriott, G., Hall, A. and Yin, H.L. (2000) Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3. Current Biology, 10(6), pp. 311-320. (doi: 10.1016/S0960-9822(00)00384-5) (PMID:10744973)
Chubb, J. R., Wilkins, A., Thomas, G. M. and Insall, R. H. (2000) The Dictyostelium RasS protein is required for macropinocytosis, phagocytosis and the control of cell movement. Journal of Cell Science, 113(4), pp. 709-719. (PMID:10652263)
Loomis, W. F. and Insall, R. H. (1999) A cell for all reasons. Nature, 401(6752), pp. 440-441. (doi: 10.1038/46713) (PMID:10519543)
Lee, S., Parent, C. A., Insall, R. and Firtel, R. A. (1999) A novel Ras-interacting protein required for chemotaxis and cyclic adenosine monophosphate signal relay in Dictyostelium. Molecular Biology of the Cell, 10(9), pp. 2829-2845. (doi: 10.1091/mbc.10.9.2829) (PMID:10473630) (PMCID:PMC25521)
Machesky, L. M. and Insall, R. H. (1999) Signaling to actin dynamics. Journal of Cell Biology, 146(2), pp. 267-272. (doi: 10.1083/jcb.146.2.267) (PMID:10427083) (PMCID:PMC2156183)
Insall, R. (1999) PKB starts to jump. Trends in Genetics, 15(7), p. 260. (doi: 10.1016/S0168-9525(99)01802-8)
Insall, R. and Machesky, L. (1999) PH domains in WASP – a bug in the system? Trends in Cell Biology, 9(6), p. 211. (doi: 10.1016/S0962-8924(99)01569-X) (PMID:10354565)
Insall, R. (1999) Cynicism and credulity. Current Biology, 9(7), R231. (doi: 10.1016/S0960-9822(99)80147-X) (PMID:10209127)
Machesky, L. M. and Insall, R. H. (1998) Scar1 and the related Wiskott–Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex. Current Biology, 8(25), pp. 1347-1356. (doi: 10.1016/S0960-9822(98)00015-3) (PMID:9889097)
Insall, R. (1998) Keeping it in the family. Current Biology, 8(12), R401. (doi: 10.1016/S0960-9822(98)70258-1)
Machesky, L. M. , Insall, R. H. and Kay, R. R. (1998) The helC gene encodes a putative DEAD-box RNA helicase required for development in Dictyostelium discoideum. Current Biology, 8(10), 607-S4. (doi: 10.1016/S0960-9822(98)70233-7) (PMID:9601648)
Insall, R. (1997) The right stuff. Current Biology, 7(11), R665. (doi: 10.1016/S0960-9822(06)00345-9) (PMID:9382817)
Tuxworth, R. I., Cheetham, J. L., Machesky, L. M. , Spiegelmann, G. B., Weeks, G. and Insall, R. H. (1997) Dictyostelium RasG is required for normal motility and cytokinesis, but not growth. Journal of Cell Biology, 138(3), pp. 605-614. (doi: 10.1083/jcb.138.3.605) (PMID:9245789) (PMCID:PMC2141629)
Insall, R. (1997) The talk's the thing. Current Biology, 7(6), R331. (doi: 10.1016/S0960-9822(06)00161-8)
Aubry, L., Maeda, M., Insall, R. , Devreotes, P. N. and Firtel, R. A. (1997) The Dictyostelium mitogen-activated protein kinase ERK2 is regulated by Ras and cAMP-dependent protein kinase (PKA) and mediates PKA function. Journal of Biological Chemistry, 272(7), pp. 3883-3886. (doi: 10.1074/jbc.272.7.3883) (PMID:9020088)
Insall, R. (1997) The enemy within. Current Biology, 7(2), R59. (doi: 10.1016/S0960-9822(06)00032-7) (PMID:9081661)
Insall, R. H. , Borleis, J. and Devreotes, P. N. (1996) The aimless RasGEF is required for processing of chemotactic signals through G-protein-coupled receptors in Dictyostelium. Current Biology, 6(6), pp. 719-729. (doi: 10.1016/S0960-9822(09)00453-9) (PMID:8793298)
Insall, R. H. (1996) Osmoregulation: cyclic GMP and the big squeeze. Current Biology, 6(5), pp. 516-518. (doi: 10.1016/S0960-9822(02)00530-4) (PMID:8805270)
Maeda, M., Aubry, L., Insall, R. , Gaskins, C., Devreotes, P. N. and Firtel, R. A. (1996) Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium. Journal of Biological Chemistry, 271(7), pp. 3351-3354. (doi: 10.1074/jbc.271.7.3351) (PMID:8631932)
Chen, M.-Y., Insall, R. H. and Devreotes, P. N. (1996) Signaling through chemoattractant receptors in Dictyostelium. Trends in Genetics, 12(2), pp. 52-57. (doi: 10.1016/0168-9525(96)81400-4) (PMID:8851971)
Insall, R. (1996) And now it's time for tea. Current Biology, 6(2), p. 99. (doi: 10.1016/S0960-9822(02)00427-X) (PMID:8859322)
Insall, R. (1995) Glycogen synthase kinase and Dictyostelium development: old pathways pointing in new directions? Trends in Genetics, 11(2), pp. 37-39. (doi: 10.1016/S0168-9525(00)88989-1) (PMID:7716803)
Insall, R. , Kuspa, A., Lilly, P. J., Shaulsky, G., Levin, L. R., Loomis, W. F. and Devreotes, P. (1994) CRAC, a cytosolic protein containing a pleckstrin homology domain, is required for receptor and G protein-mediated activation of adenylyl cyclase in Dictyostelium. Journal of Cell Biology, 126(6), pp. 1537-1545. (doi: 10.1083/jcb.126.6.1537) (PMID:8089184) (PMCID:PMC2290948)
Soede, R. D.M., Insall, R. H. , Devreotes, P. N. and Schaap, P. (1994) Extracellular cAMP can restore development in Dictyostelium cells lacking one, but not two subtypes of early cAMP receptors (cARs). Evidence for involvement of cAR1 in aggregative gene expression. Development, 120(7), pp. 1997-2002. (PMID:7925004)
Insall, R. H. , Soede, R. D.M., Schaap, P. and Devreotes, P. N. (1994) Two cAMP receptors activate common signaling pathways in Dictyostelium. Molecular Biology of the Cell, 5(6), pp. 703-711. (doi: 10.1091/mbc.5.6.703) (PMID:7949426) (PMCID:PMC301085)
Pupillo, M., Insall, R. , Pitt, G. S. and Devreotes, P. N. (1992) Multiple cyclic AMP receptors are linked to adenylyl cyclase in Dictyostelium. Molecular Biology of the Cell, 3(11), pp. 1229-1234. (doi: 10.1091/mbc.3.11.1229) (PMID:1333842) (PMCID:PMC275689)
Insall, R. , Nayler, O. and Kay, R. R. (1992) DIF-1 induces its own breakdown in Dictyostelium. EMBO Journal, 11(8), pp. 2849-2854. (PMID:1322291) (PMCID:PMC556764)
Ceccarelli, A., Mahbubani, H. J., Insall, R. , Schnitzler, G., Firtel, R. A. and Williams, J. G. (1992) A G-rich sequence element common to Dictyostelium genes which differ radically in their patterns of expression. Developmental Biology, 152(1), pp. 188-193. (doi: 10.1016/0012-1606(92)90169-H) (PMID:1321061)
Berks, M., Traynor, D., Carrin, I., Insall, R. H. and Kay, R. R. (1991) Diffusible signal molecules controlling cell differentiation and patterning in Dictyostelium. Development, 113(S1), pp. 131-139. (PMID:1660326)
Insall, R. and Kay, R. R. (1990) A specific DIF binding protein in Dictyostelium. EMBO Journal, 9(10), pp. 3323-3328. (PMID:2170113) (PMCID:PMC552069)
Book Sections
Singh, S. P. and Insall, R. H. (2022) Under-agarose chemotaxis and migration assays for dictyostelium. In: Chang, C. and Wang, J. (eds.) Cell Polarity Signaling: Methods and Protocols. Series: Methods in Molecular Biology, 2438. Humana Press: New York, pp. 467-482. ISBN 9781071620342 (doi: 10.1007/978-1-0716-2035-9_27)
Siebert, S. , Insall, R. and Machesky, L. M. (2019) More means less: managing overflow in science publishing. In: Czarniawska, B. and Löfgren, O. (eds.) Overwhelmed by Overflows? How People and Organizations Create and Manage Excess. Lund University Press: Lund, pp. 130-150. ISBN 9789198469813 (doi: 10.7765/9789198469813.00013)
Susanto, O., Muinonen-Martin, A. J., Nobis, M. and Insall, R. H. (2016) Visualizing cancer cell chemotaxis and invasion in 2D and 3D. In: Jin, T. and Hereld, D. (eds.) Chemotaxis. Series: Methods in molecular biology, 1407 (1407). Springer, pp. 217-228. ISBN 9781493934782 (doi: 10.1007/978-1-4939-3480-5_17)
Blagg, S. and Insall, R. (2004) Scar, WASp and the Arp2/3 complex in Dictyostelium migration. In: Ridley, A., Peckham, M. and Clark, P. (eds.) Cell Motility: From Molecules to Organisms. Wiley: Chichester, pp. 247-260. ISBN 9780470848722 (doi: 10.1002/0470011742.ch16)
Book Reviews
Insall, R. H. (2001) The whole organism, and nothing but the organism. Cell, 107(3), pp. 279-281. (doi: 10.1016/S0092-8674(01)00558-X)[Book Review]
Insall, R. (1996) The art of MBoC by B.A. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts and J. Watson. Trends in Genetics, 12(10), p. 430. (doi: 10.1016/0168-9525(96)81491-0)[Book Review]
Conference or Workshop Item
Husmeier, D. , Ferguson, E. , Matthiopoulos, J. and Insall, R. (2017) Statistical Inference of the Drivers of Collective Cell Movement. RSS 2017 Annual Conference, Glasgow, Scotland, 04-07 Sep 2017.
Conference Proceedings
Kim, J. and Insall, R. (2009) Position and velocity estimation of cell boundary. In: International Conference on Systems Biology, Palo Alto, USA, 30 Aug - 4 Sep 2009,
Grants
Grants and Awards listed are those received whilst working with the University of Glasgow.
- Using hybrid agent-based modelling to understand trypanosome spreading and chemotaxis.
Engineering and Physical Sciences Research Council
2023 - 2023
- Dissecting SCAR/WAVE complex activation
Medical Research Council
2023 - 2028
- Resubmission: Understanding The Physiology Of Chemotaxis Towards Self-Generated Gradients, using Models, Model Organisms and T cells
Wellcome Trust
2021 - 2026
- Integrating digital pathology and genomic analysis to optimise and streamline colonic surveillance within the Scottish Bowel Screening Programme (SBoSP) to enable early detection of Colorectal neoplasia.
Innovate UK
2020 - 2023
- EPSRC Centre for Multiscale soft tissue mechanics with MIT and POLIMI (SofTMech-MP)
Engineering and Physical Sciences Research Council
2020 - 2023
- Harnessing the physics of light to reveal mechanisms of multicellular shape and movement
Engineering and Physical Sciences Research Council
2019 - 2022
- EPSRC Centre for Multiscale soft tissue mechanics with application to heart & cancer
Engineering and Physical Sciences Research Council
2016 - 2020
- Understanding Cancer Metastasis by Combining Models, Numbers and Molecules
Cancer Research UK
2015 - 2019
- Chemotaxis in melanoma metastasis.
Wellcome Trust
2011 - 2013
- Computational modelling of cell movement and chemotaxis
Medical Research Council
2009 - 2010
Research datasets
2020
Zickus, V., Wu, M.-L., Morimoto, K., Kapitany, V., Fatima, A., Turpin, A. , Insall, R. , Whitelaw, J., Machesky, L. , Bruschini, C., Faccio, D. and Charbon, E. (2020) Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation. [Data Collection]
Tweedy, L., Thomason, P., Paschke, P., Martin, K., Machesky, L. , Zagnoni, M. and Insall, R. (2020) Seeing around corners: cells solve mazes and respond at a distance using attractant breakdown. [Data Collection]
2016
Tweedy, L., Knecht, D. A., Mackay, G. M. and Insall, R. H. (2016) Self-generated chemoattractant gradients: Attractant depletion extends the range and robustness of chemotaxis. [Data Collection]