Services

Digital Pathology

Within the GRTF lab, we have 2 high-resolution digital slide scanners.  Both offer high throughput digitalization of brightfield microscopy slides, z-stacking focusing and high-resolution image quality.  Furthermore, the most recent installation, the S60 Hamamatsu Nanozoomer, is able to scan up to 9 fluorescent channels and large megaslides. These images can be used for analysis, in publications, conferences and can be shared, anonymously, with colleagues for collaboration.  We will provide a DTA for sharing images and if you wish to access any images already stored, you can apply for approval via the Biorepository.

The images are securely archived on dedicated servers, which are supported by UoG IT department.  They can be accessed by the NDPi viewer (free to download from Hamamatsu website) or through our own dedicated software analysis, VisioPharm.

Image of S60 NanoZoomer Scanner and digital image multiplex IHC analysis of breast carcinoma


Image Analysis

The Visiopharm software enables histopathology image analysis and allows AI and deep learning to quantify and analyse tissue for research.  At the GTRF we can perform image analysis on stained slides and, in addition, we can create bespoke image analysis APPs which are tailored to your research needs.  More information about Visiopharm can be found here.  If you would like to know more about what we offer please contact us.

Image of PD-L1 RISH, lung cancer, TME


Tissue MicroArray Construction (TMAs)

The GTRF can produce bespoke TMAs for individual research needs or for general use where we act as custodian of the blocks and section as required.  TMAs are constructed from approximately 250 individual cases which are retrieved from pathological archives and which have been ethically approved through the Bio-Repository.  Using the 3DHISTECH TMA Grandmaster (only one in Scotland) cores are removed from these ‘donor’ blocks and placed into a ‘recipient block, creating a whole new TMA block.  This technique allows for multiple cases to be stained and analysed on one slide.

3DHISTECH TMA Grandmaster and samples of a tissue microarray


Histology Services

The GTRF also offers sectioning and staining of blocks.  We can carry out H&E staining on a PFM Medical Slide Stainer SS-30 which can perform H&S staining on multiple slides in under 20 minutes.  Furthermore, we can perform IHC staining using a high-throughput Thermofisher autostainer.  Antibodies can be optimized if not already worked-up, specific requirements can be discussed beforehand please use the contact us link to find out more.

SS-30 Autostainer and H&E stain of colon biopsy