INTRODUCTION TO PYTHON FOR BIOLOGISTS
Python is a dynamic, readable language that is a popular platform for all types of bioinformatics work, from simple one-off scripts to large, complex software projects. This workshop is aimed at complete beginners and assumes no prior programming experience. It gives an overview of the language with an emphasis on practical problem-solving, using examples and exercises drawn from various aspects of bioinformatics work. The workshop is structured so that the parts of the language most useful for bioinformatics are introduced as early as possible, and that students can start writing plausibly-useful programs after the first few sessions. After completing the workshop, students should be in a position to (1) apply the skills they have learned to tackling problems in their own research and (2) continue their Python education in a self-directed way.
"Great learning from someone with a biology background rather than computer science - meant we were on the same wavelength." (February 2016)
"Brilliantly well-run. From the book; to the USB stick with all the files we need; to the topics covered; to the system of helping us one-on-one, I cannot fault this course." (February 2016)
The workshop is delivered over ten half-day sessions. Each session consists of roughly a one hour lecture followed by two hours of practical exercises, with breaks at the organiser’s discretion. Each session uses examples and exercises that build on material from the previous one, so it’s important that students attend all sessions. A description of the sessions can be found at the bottom of this page.
WHO SHOULD ATTEND
This workshop is aimed at researchers and technical workers with a background in biology who want to learn programming. The syllabus has been planned with complete beginners in mind; people with previous programming experience are welcome to attend as a refresher but may find the pace a bit slow. If in doubt, take a look at the detailed session content below.
Students should have enough biological/bioinformatics background to appreciate the examples and exercise problems (i.e. they should know what a protein accession number, BLAST report, and FASTA sequence is). No previous programming experience or computer skills (beyond the ability to use a text editor) are necessary.
Module 1: Introduction
We will start with a general introduction to Python and explain why it is useful and how learning to program can benefit your research. Some time will be taken to explain the format of the course. We will outline the edit-run-fix cycle of software development and talk about how to avoid common text editing errors. In this session, we also check that the computing infrastructure for the rest of the course is in place.
Core concepts introduced: source code; text editors; whitespace; syntax and syntax error; and Python versions.
Module 2: Output and text manipulation
This session will show students how to write very simple programs that produce output to the terminal and in doing so become comfortable with editing and running Python code. This session also introduces many of the technical terms that we’ll rely on in future sessions. We will run through some examples of tools for working with text and show how they work in the context of biological sequence manipulation. We also cover different types of errors and error messages and learn how to go about fixing them methodically.
Core concepts introduced: terminals; standard output; variables and naming; strings and characters; special characters; output formatting; statements; functions; methods; arguments; comments.
Module 3: File IO and user interfaces
We will discuss about the importance of files in bioinformatics pipelines and workflows during this session, and we then explore the Python interfaces for reading from and writing to files. This involves introducing the idea of types and objects and a bit of discussion about how Python interacts with the operating system. The practical session is spent combining the techniques from session 2 with the file IO tools to create basic file-processing scripts.
Core concepts introduced: objects and classes; paths and folders; relationships between variables and values; text and binary files; newlines.
Module 4: Flow control 1: loops
A discussion of the limitations of the techniques learned in session 3 quickly reveals that flow control is required to write more sophisticated file-processing programs, at this point we will progress on to the concept of loops. We look at the way in which Python loops work, and how they can be used in a variety of contexts.
We explore the use of loops and lists together to tackle some more difficult problems.
Core concepts introduced: lists and arrays; blocks and indentation; variable scoping; iteration and the iteration interface; ranges.
Module 5: Flow control 2: conditionals
We will use the idea of decision-making in session 5 as a way to introduce conditional tests and outline the different building-blocks of conditions before showing how conditions can be combined in an expressive way. We look at the different ways that we can use conditions to control program flow, and how we can structure conditions to keep programs readable.
Core concepts introduced: Truth and falsehood; Boolean logic; identity and equality; evaluation of statements; branching.
Module 6: Organizing and structuring code
In session 6 we will discuss functions that we would like to see in Python before considering how we can add to our computational toolbox by creating our own. We examine the nuts and bolts of writing functions before looking at best-practice ways of making them usable. We also look at a couple of advanced features of Python – named arguments and defaults.
Core concepts introduced: argument passing; encapsulation; data flow through a program.
Module 7: Regular expressions
A range of common problems in bioinformatics can be described in terms of pattern matching; we will discuss these and give an overview of Python’s regex tools. We look at the building blocks of regular expressions themselves, and learn how they are a general solution to the problem of describing patterns in strings, before practising writing some specific examples of regular expressions.
Core concepts introduced: domain-specific languages; sessions and namespaces.
Module 8: Dictionaries
We discuss a few examples of key-value data and see how the problem of storing them is a common one across bioinformatics and programming in general. We learn about the syntax for dictionary creation and manipulation before talking about the situations in which dictionaries are a better fit that the data structures we have learned about thus far.
Core concepts introduced: paired data types; hashing; key uniqueness; argument unpacking and tuples.
Module 9: Interaction with the file system
In the final session e discuss the role of Python in the context of a bioinformatics workflow, and how it is often used as a language to “glue” various other components together. We then look at the Python tools for carrying out file and directory manipulation, and for running external programs – two tasks that are often necessary in order to integrate our own programs with existing ones.
Core concepts introduced: processes and sub-processes; the shell and shell utilities; program return values.
REGISTRATION FEE: £535 (includes coffee/tea, but no lunch)
5 full day sessions – 9th-13th Sept 2019
If you would like to sign up to this course, please email Polyomics@glasgow.ac.uk with a budget code for charging and we will add your name to the register.
First published: 24 July 2019