Imaging at the CVR

The Centre for Virus Research (CVR) is equipped with two state-of-the-art Zeiss confocal microscopes: the LSM 710 Meta and the cutting-edge LSM 880.

Both systems are operated via Zen software and fitted with high NA oil immersion objectives and laser lines, with excitation at 405, 458, 488, 514, 561 and 633 nm, and at 594nm for the LSM 880.

The LSM 710 Meta’s strength is in its flexibility, utilising a spectral detector to remove autofluorescence and unmix up to 10 fluorophores, even when spectrally similar.

However, the enhanced quantum efficiency of the LSM 880’s GaAsP detector provides significant improvement in signal-to-noise ratio in comparison to the LSM 710, the newer system also offering improved scan speed and facilitating super-resolution microscopy through utilisation of the Airyscan detector.

Offline workstations with Zen software and Bitplane’s Imaris software allow post-acquisition 3D modelling and object quantitation.

For further information contact: Colin.Loney@glasgow.ac.uk - 0141 330 4025