The Hutchinson group
Research Programme:
Influenza viruses cause seasonal and pandemic influenza in humans as well as outbreaks of disease in domesticated animals. We are interested in their molecular biology, particularly the ways in which viral and host proteins function together during an infection. Our work aims to relate changes in influenza virion composition to infectivity, and to examine how viral replication is regulated by chemical modifications of proteins.
Current Research
Relating the protein composition of influenza virions to their ability to infect cells.
This builds on a study of influenza virions which obtained the first complete and quantified description of their protein composition (Hutchinson et al. (2014) Nature Communications).
We are now interested in exploring how changes in virion composition – due, for example, to changes in the host species – alter infectivity. We are also interested in similarities between the composition of virions and of microvesicles shed naturally by the host, and in whether microvesicles therefore play a role in influenza infections.
Characterising Filamentous Influenza Viruses
Laboratory-adapted strains of influenza virus typically form spherical virions. In contrast, clinical isolates are also able to produce extremely long filamentous virions. These filaments have been noted in the literature for many years, but most research has concentrated on spherical virions and the distinctive structure and functional importance of filaments has only recently become apparent (see Dadonaite et al. (2016) Journal of General Virology). In collaboration with the Bhella group we are working to characterise the composition of influenza filaments and relate this to their functions in an infection.
Determining how changes in chemical modifications regulate viral replication
We are examining how post-translational modifications of viral and host proteins regulate cellular signalling during infection, and hope to identify ways of targeting this to limit viral replication. This builds on work which identified specific sites of protein phosphorylation on viral proteins (Hutchinson et al. (2012) PLOS Pathogens) and linked this to specific regulatory roles of phosphorylation in infection (e.g. Turrell et al. (2015) Journal of Virology).
Resources
A protocol for influenza virion purification can be found here.
Mass spectra from published studies can be downloaded from MassIVE using the IDs MSV000078740 and MSV000078741.
Research group members
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Jack Hirst
Lea Meyer |
Seema Jasim
Elizabeth Sloan
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Collaborators
- Dr David Bhella - virus structure
- Dr Chris Boutell - intrinsic immunity
