Dr Niall Macquaide

  • Research Fellow (Institute of Cardiovascular and Medical Sciences)

telephone: 01413304766
email: Niall.Macquaide@glasgow.ac.uk

Biography

I graduated from Glasgow in 2001 with an honours degree in Physiology and went on to study for a PhD. Working with Prof. Godfrey Smith, I investigating spontaneous Ca2+ release in cardiac myocytes through experimental and computer modelling techniques. After attaining my PhD in 2005, I carried out postdoctoral work examining the electrophysiological remodelling which occurs through the progression of heart failure in a rabbit model of myocardial infarction. Through this work I carried out work in the lab of Prof Donald Bers in UC Davis, California.  In 2009 I attained a Marie Curie Fellowship to work in Leuven, Belgium in the Lab of Karin Sipido to study how RyR cluster organisation is altered during atrial fibrillation. In addition to the core study, I co-supervised two PhD students and attained new skills in Super Resolution Microscopy. I currently hold a BHF intermediate research fellowship and a Marie Curie reintegration fellowship.

Research interests

Member: Cardiac Research

I am interested in the functional alterations in cardiac cells after remodelled after myocardial infarction. I use advanced imaging methodologies and analysis methods, to study Ca2+ subcellular Ca2+ release in living cells as well as super resolution microscopy to measure alterations in the organisation of cellular proteins.

Ryanodine Receptor clustering. Using super resolution microscopy methods, I will measure clustering of ryanodine receptors (RyRs) with single protein accuracy and assess changes coincident with pathological remodelling following myocardial infarction (MI). Ca2+ release from individual clusters will be imaged using a high speed, highly sensitive confocal imaging system. The goal here is to assess the changes which occur in failing hearts, how this can lead to an arrhythmogenic phenotype and to determine novel therapeutic strategies.

Ca2+-calmodulin-dependant kinease (CaMKII). CaMKII is shown to induce a “hyperphosphorylation” of RyR, with known pro-arrhythmogenic effects in MI. Using in-vitro transfection of primary isolated myocytes I will introduce genetically encoded probes to assess the activation status of this kinase and how this changes in an arrhythmogenic phenotype. In collaboration with my collaborators: Prof Donald M Bers & Dr Julie Bossuyt, UC Davis, CA I will determine how local Ca2+ gradients and CaMKII localisation may affect cellular function and changes in these activities within the context of pathological hypertrophic remodelling. 

Cytoskeletal proteins. The role of a relevant cytoskeletal protein (Juncophilin-2), which is thought to tether the surface membrane to that of the cell’s Ca2+ store will then be targeted to assess it’s role in the creation of identified Ca2+ signalling microdomains. An siRNA gene silencing virus against junctophilin-2 has been created by one of my collaborators (Dr X Wehrens, Baylor, TX, USA) and this will be used to study how loss of this protein disrupts Ca2+ signalling microdomain, as both down regulation of junctophilin-2 and irregular Ca2+ release are also observed after remodelling in several cardiac pathologies.

Atrial structural remodelling after MI. In collaboration with Dr. Anthony Workman, I am investigating structural alterations in t-tubule membrane in patients with and without MI to investigate how this could be changes by post-MI remodelling.

The role of PDE’s in the heart. In collaboration with Prof George Baillie, I am investigating the role of PDEs in the regulation of key players of cardiac calcium release and uptake. Using biochemical and physiological techniques to explore the interaction of these PDEs with RyR and SERCA/ phospholamban (PLB), to develop more targeted therapies in the future. 

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Cardiocluster
    European Commission
    2014 - 2018
     

Publications

List by: Type | Date

Jump to: 2015 | 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006
Number of items: 24.

2015

MacQuaide, N. , Tuan, H.-T. M., Hotta, J.-I., Sempels, W., Lenaerts, I., Holemans, P., Hofkens, J., Jafri, M. S., Willems, R. and Sipido, K. R. (2015) Ryanodine receptor cluster fragmentation and redistribution in persistent atrial fibrillation enhance calcium release. Cardiovascular Research, 108(3), pp. 387-398. (doi:10.1093/cvr/cvv231) (PMID:26490742) (PMCID:PMC4648199)

Sipido, K. R., MacQuaide, N. and Bito, V. (2015) A systematic approach for assessing Ca²⁺ handling in cardiac myocytes. Cold Spring Harbor Protocols, 2015(5), pp. 431-433. (doi:10.1101/pdb.top066142) (PMID:25934941)

Bito, V., Sipido, K. R. and MacQuaide, N. (2015) Assessing Ca²⁺-removal pathways in cardiac myocytes. Cold Spring Harbor Protocols, 2015(5), pp. 498-503. (doi:10.1101/pdb.prot076992) (PMID:25934931)

MacQuaide, N. , Bito, V. and Sipido, K. R. (2015) Measuring Ca²⁺ sparks in cardiac myocytes. Cold Spring Harbor Protocols, 2015(5), pp. 490-7. (doi:10.1101/pdb.prot076984) (PMID:25934930)

Smith, G. L. and MacQuaide, N. (2015) The direct actions of flecainide on the human cardiac ryanodine receptor: keeping open the debate on the mechanism of action of local anesthetics in CPVT. Circulation Research, 116(8), pp. 1284-1286. (doi:10.1161/CIRCRESAHA.115.306298) (PMID:25858058)

Bito, V., MacQuaide, N. and Sipido, K. R. (2015) Characterizing the trigger for sarcoplasmic reticulum Ca2+ release in cardiac myocytes. Cold Spring Harbor Protocols, 2015(4), pp. 398-402. (doi:10.1101/pdb.prot076968) (PMID:25834258)

Bito, V., Sipido, K. R. and MacQuaide, N. (2015) Basic methods for monitoring intracellular Ca2+ in cardiac myocytes using Fluo-3. Cold Spring Harbor Protocols, 2015(4), pp. 392-397. (doi:10.1101/pdb.prot076950) (PMID:25834257)

2013

Dries, E., Bito, V., Lenaerts, I., Antoons, G., Sipido, K. R. and MacQuaide, N. (2013) Selective modulation of coupled ryanodine receptors during microdomain activation of calcium/calmodulin-dependent kinase ii in the dyadic cleft. Circulation Research, 113(11), pp. 1242-1252. (doi:10.1161/CIRCRESAHA.113.301896) (PMID:24081880)

Hohendanner, F. et al. (2013) Intracellular dyssynchrony of diastolic cytosolic [Ca2+] decay in ventricular cardiomyocytes in cardiac remodeling and human heart failure. Circulation Research, 113(5), pp. 527-538. (doi:10.1161/CIRCRESAHA.113.300895) (PMID:23825358)

2012

Sipido, K. R., Acsai, K., Antoons, G., Bito, V. and MacQuaide, N. (2012) T-tubule remodelling and ryanodine receptor organization modulate sodium-calcium exchange. Advances in Experimental Medicine and Biology, 961, pp. 375-83. (doi:10.1007/978-1-4614-4756-6_32) (PMID:23224896)

Kemi, O. J., MacQuaide, N. , Hoydal, M. A., Ellingsen, O., Smith, G. L. and Wisloff, U. (2012) Exercise training corrects control of spontaneous calcium waves in hearts from myocardial infarction heart failure rats. Journal of Cellular Physiology, 227(1), pp. 20-26. (doi:10.1002/jcp.22771)

Bito, V., Biesmans, L., Gellen, B., Antoons, G., MacQuaide, N. , Rouet-Benzineb, P., Pezet, M., Mercadier, J.-J. and Sipido, K. R. (2012) FKBP12.6 overexpression does not protect against remodelling after myocardial infarction. Experimental Physiology, 98(1), pp. 134-48. (doi:10.1113/expphysiol.2011.064089) (PMID:22689442)

2011

Kemi, O. J., Hoydal, M. A., MacQuaide, N. , Haram, P. M., Koch, L. G., Britton, S. L., Ellingsen, O., Smith, G. L. and Wisloff, U. (2011) The effect of exercise training on transverse tubules in normal, remodeled, and reverse remodeled hearts. Journal of Cellular Physiology, 226(9), pp. 2235-2243. (doi:10.1002/jcp.22559)

Heinzel, F. R., MacQuaide, N. , Biesmans, L. and Sipido, K. (2011) Dyssynchrony of Ca2+ release from the sarcoplasmic reticulum as subcellular mechanism of cardiac contractile dysfunction. Journal of Molecular and Cellular Cardiology, 50(3), pp. 390-400. (doi:10.1016/j.yjmcc.2010.11.008) (PMID:21075114)

MacQuaide, N. and Sipido, K. R. (2011) Blink and you'll see it: how to detect Ca2+ quarks. Circulation Research, 108(2), pp. 154-156. (doi:10.1161/CIRCRESAHA.110.238766) (PMID:21252146)

Rota, M., Biesmans, L., MacQuaide, N. , Heinzel, F.R., Bito, V., Smith, G.L. and Sipido, K.R. (2011) Subcellular heterogeneity of ryanodine receptor properties in ventricular myocytes with low T-tubule density. PLoS ONE, 6(10), e25100. (doi:10.1371/journal.pone.0025100)

2010

MacQuaide, N. , Ramay, H.R., Sobie, E.A. and Smith, G.L. (2010) Differential sensitivity of Ca2+ wave and Ca2+ spark events to ruthenium red in isolated permeabilised rabbit cardiomyocytes. Journal of Physiology, 588(23), pp. 4731-4742. (doi:10.1113/jphysiol.2010.193375)

Zamparelli, C., MacQuaide, N. , Colotti, G., Verzili, D., Seidler, T., Smith, G. L. and Chiancone, E. (2010) Activation of the cardiac Na+–Ca2+ exchanger by sorcin via the interaction of the respective Ca2+-binding domains. Journal of Molecular and Cellular Cardiology, 49(1), pp. 132-141. (doi:10.1016/j.yjmcc.2010.03.003) (PMID:20298697) (PMCID:PMC2958309)

2009

MacQuaide, N. , Dempster, J. and Smith, G. L. (2009) Assessment of sarcoplasmic reticulum Ca2+ depletion during spontaneous Ca2+ waves in isolated permeabilized rabbit ventricular cardiomyocytes. Biophysical Journal, 96(7), pp. 2744-2754. (doi:10.1016/j.bpj.2008.12.3944) (PMID:19348757) (PMCID:PMC2711300)

2008

Smith, G. and MacQuaide, N. (2008) Cytoplasmic versus intra-SR: the battle of the Ca2+ diffusion coefficients in cardiac muscle. Biophysical Journal, 95(3), pp. 1005-1006. (doi:10.1529/biophysj.108.133926) (PMID:18469088) (PMCID:PMC2479579)

Adamson, M., MacQuaide, N. , Helgerud, J., Hoff, J. and Kemi, O.J. (2008) Unilateral arm strength training improves contralateral peak force and rate of force development. European Journal of Applied Physiology, 103(5), pp. 553-559. (doi:10.1007/s00421-008-0750-6) (PMID:18443814)

2007

MacQuaide, N. , Dempster, J. and Smith, G.L. (2007) Measurement and modeling of Ca2+ waves in isolated rabbit ventricular cardiomyocytes. Biophysical Journal, 93(7), pp. 2581-2595. (doi:10.1529/biophysj.106.102293) (PMID:17545234) (PMCID:PMC1965444)

Volkers, M. et al. (2007) S100A1 decreases calcium spark frequency and alters their spatial characteristics in permeabilized adult ventricular cardiomyocytes. Cell Calcium, 41(2), pp. 135-143. (doi:10.1016/j.ceca.2006.06.001) (PMID:16919727)

2006

Smith, G. and MacQuaide, N. (2006) Swallowing a spider to catch a fly: ca-calmodulin dynamics in adult cardiomyocytes. Journal of Molecular and Cellular Cardiology, 41(3), pp. 416-417. (doi:10.1016/j.yjmcc.2006.06.008) (PMID:16870208)

This list was generated on Sat Oct 21 10:20:41 2017 BST.
Jump to: Articles
Number of items: 24.

Articles

MacQuaide, N. , Tuan, H.-T. M., Hotta, J.-I., Sempels, W., Lenaerts, I., Holemans, P., Hofkens, J., Jafri, M. S., Willems, R. and Sipido, K. R. (2015) Ryanodine receptor cluster fragmentation and redistribution in persistent atrial fibrillation enhance calcium release. Cardiovascular Research, 108(3), pp. 387-398. (doi:10.1093/cvr/cvv231) (PMID:26490742) (PMCID:PMC4648199)

Sipido, K. R., MacQuaide, N. and Bito, V. (2015) A systematic approach for assessing Ca²⁺ handling in cardiac myocytes. Cold Spring Harbor Protocols, 2015(5), pp. 431-433. (doi:10.1101/pdb.top066142) (PMID:25934941)

Bito, V., Sipido, K. R. and MacQuaide, N. (2015) Assessing Ca²⁺-removal pathways in cardiac myocytes. Cold Spring Harbor Protocols, 2015(5), pp. 498-503. (doi:10.1101/pdb.prot076992) (PMID:25934931)

MacQuaide, N. , Bito, V. and Sipido, K. R. (2015) Measuring Ca²⁺ sparks in cardiac myocytes. Cold Spring Harbor Protocols, 2015(5), pp. 490-7. (doi:10.1101/pdb.prot076984) (PMID:25934930)

Smith, G. L. and MacQuaide, N. (2015) The direct actions of flecainide on the human cardiac ryanodine receptor: keeping open the debate on the mechanism of action of local anesthetics in CPVT. Circulation Research, 116(8), pp. 1284-1286. (doi:10.1161/CIRCRESAHA.115.306298) (PMID:25858058)

Bito, V., MacQuaide, N. and Sipido, K. R. (2015) Characterizing the trigger for sarcoplasmic reticulum Ca2+ release in cardiac myocytes. Cold Spring Harbor Protocols, 2015(4), pp. 398-402. (doi:10.1101/pdb.prot076968) (PMID:25834258)

Bito, V., Sipido, K. R. and MacQuaide, N. (2015) Basic methods for monitoring intracellular Ca2+ in cardiac myocytes using Fluo-3. Cold Spring Harbor Protocols, 2015(4), pp. 392-397. (doi:10.1101/pdb.prot076950) (PMID:25834257)

Dries, E., Bito, V., Lenaerts, I., Antoons, G., Sipido, K. R. and MacQuaide, N. (2013) Selective modulation of coupled ryanodine receptors during microdomain activation of calcium/calmodulin-dependent kinase ii in the dyadic cleft. Circulation Research, 113(11), pp. 1242-1252. (doi:10.1161/CIRCRESAHA.113.301896) (PMID:24081880)

Hohendanner, F. et al. (2013) Intracellular dyssynchrony of diastolic cytosolic [Ca2+] decay in ventricular cardiomyocytes in cardiac remodeling and human heart failure. Circulation Research, 113(5), pp. 527-538. (doi:10.1161/CIRCRESAHA.113.300895) (PMID:23825358)

Sipido, K. R., Acsai, K., Antoons, G., Bito, V. and MacQuaide, N. (2012) T-tubule remodelling and ryanodine receptor organization modulate sodium-calcium exchange. Advances in Experimental Medicine and Biology, 961, pp. 375-83. (doi:10.1007/978-1-4614-4756-6_32) (PMID:23224896)

Kemi, O. J., MacQuaide, N. , Hoydal, M. A., Ellingsen, O., Smith, G. L. and Wisloff, U. (2012) Exercise training corrects control of spontaneous calcium waves in hearts from myocardial infarction heart failure rats. Journal of Cellular Physiology, 227(1), pp. 20-26. (doi:10.1002/jcp.22771)

Bito, V., Biesmans, L., Gellen, B., Antoons, G., MacQuaide, N. , Rouet-Benzineb, P., Pezet, M., Mercadier, J.-J. and Sipido, K. R. (2012) FKBP12.6 overexpression does not protect against remodelling after myocardial infarction. Experimental Physiology, 98(1), pp. 134-48. (doi:10.1113/expphysiol.2011.064089) (PMID:22689442)

Kemi, O. J., Hoydal, M. A., MacQuaide, N. , Haram, P. M., Koch, L. G., Britton, S. L., Ellingsen, O., Smith, G. L. and Wisloff, U. (2011) The effect of exercise training on transverse tubules in normal, remodeled, and reverse remodeled hearts. Journal of Cellular Physiology, 226(9), pp. 2235-2243. (doi:10.1002/jcp.22559)

Heinzel, F. R., MacQuaide, N. , Biesmans, L. and Sipido, K. (2011) Dyssynchrony of Ca2+ release from the sarcoplasmic reticulum as subcellular mechanism of cardiac contractile dysfunction. Journal of Molecular and Cellular Cardiology, 50(3), pp. 390-400. (doi:10.1016/j.yjmcc.2010.11.008) (PMID:21075114)

MacQuaide, N. and Sipido, K. R. (2011) Blink and you'll see it: how to detect Ca2+ quarks. Circulation Research, 108(2), pp. 154-156. (doi:10.1161/CIRCRESAHA.110.238766) (PMID:21252146)

Rota, M., Biesmans, L., MacQuaide, N. , Heinzel, F.R., Bito, V., Smith, G.L. and Sipido, K.R. (2011) Subcellular heterogeneity of ryanodine receptor properties in ventricular myocytes with low T-tubule density. PLoS ONE, 6(10), e25100. (doi:10.1371/journal.pone.0025100)

MacQuaide, N. , Ramay, H.R., Sobie, E.A. and Smith, G.L. (2010) Differential sensitivity of Ca2+ wave and Ca2+ spark events to ruthenium red in isolated permeabilised rabbit cardiomyocytes. Journal of Physiology, 588(23), pp. 4731-4742. (doi:10.1113/jphysiol.2010.193375)

Zamparelli, C., MacQuaide, N. , Colotti, G., Verzili, D., Seidler, T., Smith, G. L. and Chiancone, E. (2010) Activation of the cardiac Na+–Ca2+ exchanger by sorcin via the interaction of the respective Ca2+-binding domains. Journal of Molecular and Cellular Cardiology, 49(1), pp. 132-141. (doi:10.1016/j.yjmcc.2010.03.003) (PMID:20298697) (PMCID:PMC2958309)

MacQuaide, N. , Dempster, J. and Smith, G. L. (2009) Assessment of sarcoplasmic reticulum Ca2+ depletion during spontaneous Ca2+ waves in isolated permeabilized rabbit ventricular cardiomyocytes. Biophysical Journal, 96(7), pp. 2744-2754. (doi:10.1016/j.bpj.2008.12.3944) (PMID:19348757) (PMCID:PMC2711300)

Smith, G. and MacQuaide, N. (2008) Cytoplasmic versus intra-SR: the battle of the Ca2+ diffusion coefficients in cardiac muscle. Biophysical Journal, 95(3), pp. 1005-1006. (doi:10.1529/biophysj.108.133926) (PMID:18469088) (PMCID:PMC2479579)

Adamson, M., MacQuaide, N. , Helgerud, J., Hoff, J. and Kemi, O.J. (2008) Unilateral arm strength training improves contralateral peak force and rate of force development. European Journal of Applied Physiology, 103(5), pp. 553-559. (doi:10.1007/s00421-008-0750-6) (PMID:18443814)

MacQuaide, N. , Dempster, J. and Smith, G.L. (2007) Measurement and modeling of Ca2+ waves in isolated rabbit ventricular cardiomyocytes. Biophysical Journal, 93(7), pp. 2581-2595. (doi:10.1529/biophysj.106.102293) (PMID:17545234) (PMCID:PMC1965444)

Volkers, M. et al. (2007) S100A1 decreases calcium spark frequency and alters their spatial characteristics in permeabilized adult ventricular cardiomyocytes. Cell Calcium, 41(2), pp. 135-143. (doi:10.1016/j.ceca.2006.06.001) (PMID:16919727)

Smith, G. and MacQuaide, N. (2006) Swallowing a spider to catch a fly: ca-calmodulin dynamics in adult cardiomyocytes. Journal of Molecular and Cellular Cardiology, 41(3), pp. 416-417. (doi:10.1016/j.yjmcc.2006.06.008) (PMID:16870208)

This list was generated on Sat Oct 21 10:20:41 2017 BST.