Dr Rudi Prihandoko

  • Research Associate (Institute of Molecular Cell & Systems Biology)

Biography

I did my Undergraduate study in Biochemistry at the University of Wales, Cardiff in 2002. I then worked for a Biotechnology company called Molecular Light Technology Research Ltd, also in Cardiff, developing molecular biological assays to detect pathogens in clinical samples and environmental waters. In 2013, I was awarded a BBSRC/CASE studentship with Heptares Therapeutics to study a PhD in the pharmacology and regulation of muscarinic receptors under the supervision of Professor Andrew B. Tobin at the University of Leicester. During this PhD, I spent 3 months at Heptares and subsequently employed by Heptares for a further 20 months during and briefly after my writing up period.

In late 2013, I re-joined the Tobin laboratory, now at the University of Glasgow, and since then I have been working on the BBSRC funded project aimed at investigating the molecular pharmacology and physiology of the free fatty acid 4 (FFA4) receptor. This project is focused on in vitro pharmacological characterisation of novel FFA4 ligands and the use of animal models to study in vivo physiological roles of FFA4 in metabolic, inflammatory and respiratory systems. Techniques employed in this project include BRET beta-arrestin recruitment assay, ERK1/2 phosphorylation, calcium mobilisation, FFA4 phosphorylation and internalisation, immunohistochemistry and tissue isolation (precision cut lung slices and islets) and whole-body metabolic (glucose and insulin tolerance, GLP-1 release) and respiratory (lung resistance and compliance) studies.


Publications

List by: Type | Date

Jump to: 2018 | 2017 | 2016 | 2015 | 2014
Number of items: 8.

2018

Bradley, S. J. , Tobin, A. B. and Prihandoko, R. (2018) Muscarinic acetylcholine receptors in the central nervous system. Neuropharmacology, 136(Part C), p. 361. (doi: 10.1016/j.neuropharm.2018.06.012) (PMID:29894772)

Bradley, S. J. , Tobin, A. B. and Prihandoko, R. (2018) The use of chemogenetic approaches to study the physiological roles of muscarinic acetylcholine receptors in the central nervous system. Neuropharmacology, 136(Part C), pp. 421-426. (doi: 10.1016/j.neuropharm.2017.11.043) (PMID:29191752)

2017

Milligan, G. , Alvarez-Curto, E. , Hudson, B. D. , Prihandoko, R. and Tobin, A. B. (2017) FFA4/GPR120: pharmacology and therapeutic opportunities. Trends in Pharmacological Sciences, 38(9), pp. 809-821. (doi: 10.1016/j.tips.2017.06.006) (PMID:28734639) (PMCID:PMC5582618)

2016

Alvarez-Curto, E. , Inoue, A., Jenkins, L. , Raihan, S. Z., Prihandoko, R., Tobin, A. B. and Milligan, G. (2016) Targeted elimination of G proteins and arrestins defines their specific contributions to both intensity and duration of G protein-coupled receptor signalling. Journal of Biological Chemistry, 291(53), pp. 27147-27159. (doi: 10.1074/jbc.M116.754887) (PMID:27852822) (PMCID:PMC5207144)

Butcher, A. J. et al. (2016) An antibody biosensor establishes the activation of the M1 muscarinic acetylcholine receptor during learning and memory. Journal of Biological Chemistry, 291(17), pp. 8862-8875. (doi: 10.1074/jbc.M115.681726) (PMID:26826123) (PMCID:PMC4861454)

2015

Prihandoko, R., Bradley, S. J. , Tobin, A. and Butcher, A. J. (2015) Determination of GPCR phosphorylation status: establishing a phosphorylation barcode. Current Protocols in Pharmacology, 69, 2.13.1-2.13.26. (doi: 10.1002/0471141755.ph0213s69) (PMID:26344213)

2014

Tobin, A. and Prihandoko, R. (2014) Reply to "Letter to the editor: 'Systems biology versus reductionism in cell physiology'". American Journal of Physiology: Cell Physiology, 307(3), C310-C310. (doi: 10.1152/ajpcell.00192.2014) (PMID:25088763) (PMCID:PMC4121582)

Prihandoko, R. and Tobin, A. B. (2014) Challenges of assigning protein kinases to in vivo phosphorylation events. Focus on "Use of LC-MS/MS and Bayes' theorem to identify protein kinases that phosphorylate aquaporin-2 at Ser256". American Journal of Physiology: Cell Physiology, 307(2), C121-C122. (doi: 10.1152/ajpcell.00136.2014) (PMID:24829494)

This list was generated on Wed Jul 8 15:58:43 2020 BST.
Jump to: Articles
Number of items: 8.

Articles

Bradley, S. J. , Tobin, A. B. and Prihandoko, R. (2018) Muscarinic acetylcholine receptors in the central nervous system. Neuropharmacology, 136(Part C), p. 361. (doi: 10.1016/j.neuropharm.2018.06.012) (PMID:29894772)

Bradley, S. J. , Tobin, A. B. and Prihandoko, R. (2018) The use of chemogenetic approaches to study the physiological roles of muscarinic acetylcholine receptors in the central nervous system. Neuropharmacology, 136(Part C), pp. 421-426. (doi: 10.1016/j.neuropharm.2017.11.043) (PMID:29191752)

Milligan, G. , Alvarez-Curto, E. , Hudson, B. D. , Prihandoko, R. and Tobin, A. B. (2017) FFA4/GPR120: pharmacology and therapeutic opportunities. Trends in Pharmacological Sciences, 38(9), pp. 809-821. (doi: 10.1016/j.tips.2017.06.006) (PMID:28734639) (PMCID:PMC5582618)

Alvarez-Curto, E. , Inoue, A., Jenkins, L. , Raihan, S. Z., Prihandoko, R., Tobin, A. B. and Milligan, G. (2016) Targeted elimination of G proteins and arrestins defines their specific contributions to both intensity and duration of G protein-coupled receptor signalling. Journal of Biological Chemistry, 291(53), pp. 27147-27159. (doi: 10.1074/jbc.M116.754887) (PMID:27852822) (PMCID:PMC5207144)

Butcher, A. J. et al. (2016) An antibody biosensor establishes the activation of the M1 muscarinic acetylcholine receptor during learning and memory. Journal of Biological Chemistry, 291(17), pp. 8862-8875. (doi: 10.1074/jbc.M115.681726) (PMID:26826123) (PMCID:PMC4861454)

Prihandoko, R., Bradley, S. J. , Tobin, A. and Butcher, A. J. (2015) Determination of GPCR phosphorylation status: establishing a phosphorylation barcode. Current Protocols in Pharmacology, 69, 2.13.1-2.13.26. (doi: 10.1002/0471141755.ph0213s69) (PMID:26344213)

Tobin, A. and Prihandoko, R. (2014) Reply to "Letter to the editor: 'Systems biology versus reductionism in cell physiology'". American Journal of Physiology: Cell Physiology, 307(3), C310-C310. (doi: 10.1152/ajpcell.00192.2014) (PMID:25088763) (PMCID:PMC4121582)

Prihandoko, R. and Tobin, A. B. (2014) Challenges of assigning protein kinases to in vivo phosphorylation events. Focus on "Use of LC-MS/MS and Bayes' theorem to identify protein kinases that phosphorylate aquaporin-2 at Ser256". American Journal of Physiology: Cell Physiology, 307(2), C121-C122. (doi: 10.1152/ajpcell.00136.2014) (PMID:24829494)

This list was generated on Wed Jul 8 15:58:43 2020 BST.