Dr Rudi Prihandoko
- Research Associate (Institute of Molecular Cell & Systems Biology)
I did my Undergraduate study in Biochemistry at the University of Wales, Cardiff in 2002. I then worked for a Biotechnology company called Molecular Light Technology Research Ltd, also in Cardiff, developing molecular biological assays to detect pathogens in clinical samples and environmental waters. In 2013, I was awarded a BBSRC/CASE studentship with Heptares Therapeutics to study a PhD in the pharmacology and regulation of muscarinic receptors under the supervision of Professor Andrew B. Tobin at the University of Leicester. During this PhD, I spent 3 months at Heptares and subsequently employed by Heptares for a further 20 months during and briefly after my writing up period.
In late 2013, I re-joined the Tobin laboratory, now at the University of Glasgow, and since then I have been working on the BBSRC funded project aimed at investigating the molecular pharmacology and physiology of the free fatty acid 4 (FFA4) receptor. This project is focused on in vitro pharmacological characterisation of novel FFA4 ligands and the use of animal models to study in vivo physiological roles of FFA4 in metabolic, inflammatory and respiratory systems. Techniques employed in this project include BRET beta-arrestin recruitment assay, ERK1/2 phosphorylation, calcium mobilisation, FFA4 phosphorylation and internalisation, immunohistochemistry and tissue isolation (precision cut lung slices and islets) and whole-body metabolic (glucose and insulin tolerance, GLP-1 release) and respiratory (lung resistance and compliance) studies.