Dr Richard Ward

  • Research Associate (Institute of Molecular Cell & Systems Biology)

telephone: 01413306483
email: Richard.Ward@glasgow.ac.uk

Publications

List by: Type | Date

Jump to: 2019 | 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2004 | 2003
Number of items: 35.

2019

Stoneman, M. R., Biener, G., Ward, R. J., Pediani, J. D. , Badu, D., Eis, A., Popa, I., Milligan, G. and Raicu, V. (2019) A general method to quantify ligand-driven oligomerization from fluorescence-based images. Nature Methods, 16(6), pp. 493-496. (doi: 10.1038/s41592-019-0408-9) (PMID:31110281)

Milligan, G. , Ward, R. J. and Marsango, S. (2019) GPCR homo-oligomerization. Current Opinion in Cell Biology, 57, pp. 40-47. (doi: 10.1016/j.ceb.2018.10.007) (PMID:30453145)

Müskens, F. M., Ward, R. J., Herkt, D., van de Langemheen, H., Tobin, A. B. , Liskamp, R. M.J. and Milligan, G. (2019) Design, synthesis and evaluation of a diazirine photoaffinity probe for ligand-based receptor capture targeting G protein-coupled receptors. Molecular Pharmacology, 95(2), pp. 196-209. (doi: 10.1124/mol.118.114249) (PMID:30514721) (PMCID:PMC6324650)

2018

Marsango, S., Ward, R. J., Alvarez-Curto, E. and Milligan, G. (2018) Muscarinic receptor oligomerization. Neuropharmacology, 136(Part C), pp. 401-410. (doi: 10.1016/j.neuropharm.2017.11.023) (PMID:29146505) (PMCID:PMC6078712)

Liu, Y. et al. (2018) Assessing the real-time activation of the cannabinoid CB1 receptor and the associated structural changes using a FRET biosensor. International Journal of Biochemistry and Cell Biology, 99, pp. 114-124. (doi: 10.1016/j.biocel.2018.04.003) (PMID:29626639)

Liu, Y. et al. (2018) Visualization of the activation of the histamine H3 receptor (H3R) using novel fluorescence resonance energy transfer biosensors and their potential application to the study of H3R pharmacology. FEBS Journal, 285(12), pp. 2319-2336. (doi: 10.1111/febs.14484) (PMID:29701013)

Pediani, J. D. , Ward, R. J., Marsango, S. and Milligan, G. (2018) Spatial intensity distribution analysis: studies of G Protein-coupled receptor oligomerization. Trends in Pharmacological Sciences, 39(2), pp. 175-186. (doi: 10.1016/j.tips.2017.09.001) (PMID:29032835) (PMCID:PMC5783713)

2017

Ward, R. J., Marsango, S., Pediani, J. D. and Milligan, G. (2017) The use of spatial intensity distribution analysis to examine G protein-coupled receptor oligomerization. In: Herrick-Davis, K., Milligan, G. and Di Giovanni, G. (eds.) G-Protein-Coupled Receptor Dimers. Series: Receptors (33). Humana Press: Cham, pp. 15-38. ISBN 9783319601724 (doi:10.1007/978-3-319-60174-8_2)

Ward, R. J., Pediani, J. D. , Harikumar, K. G., Miller, L. J. and Milligan, G. (2017) Spatial intensity distribution analysis quantifies the extent and regulation of homodimerization of the secretin receptor. Biochemical Journal, 474(11), pp. 1879-1895. (doi: 10.1042/BCJ20170184) (PMID:28424368) (PMCID:PMC5442643)

Marsango, S., Caltabiano, G., Jiménez-Rosés, M., Millan, M. J., Pediani, J. D. , Ward, R. J. and Milligan, G. (2017) A molecular basis for selective antagonist destabilization of dopamine D3 receptor quaternary organization. Scientific Reports, 7, 2134. (doi: 10.1038/s41598-017-02249-3) (PMID:28522847) (PMCID:PMC5437050)

Guo, H., An, S., Ward, R. J., Yang, Y., Liu, Y., Guo, X.-X., Hao, Q. and Xu, T.-R. (2017) Methods used to study the oligomeric structure of G protein-coupled receptors. Bioscience Reports, 37(2), (doi: 10.1042/bsr20160547) (PMID:28062602) (PMCID:PMC5398257)

2016

Liu, Y., An, S., Ward, R., Yang, Y., Guo, X., Li, W. and Xu, T.-R. (2016) G protein-coupled receptors as promising cancer targets. Cancer Letters, 376(2), pp. 226-239. (doi: 10.1016/j.canlet.2016.03.031) (PMID:27000991)

Pediani, J. D. , Ward, R. J., Godin, A. G., Marsango, S. and Milligan, G. (2016) Dynamic regulation of quaternary organization of the M1 muscarinic receptor by subtype-selective antagonist drugs. Journal of Biological Chemistry, 291, pp. 13132-13146. (doi: 10.1074/jbc.M115.712562) (PMID:27080256) (PMCID:PMC4933229)

2015

An, S., Yang, Y., Ward, R., Liu, Y., Guo, X.-X. and Xu, T.-R. (2015) A-Raf: A new star of the family of raf kinases. Critical Reviews in Biochemistry and Molecular Biology, 50(6), pp. 520-531. (doi: 10.3109/10409238.2015.1102858) (PMID:26508523)

Liste, M. J., Caltabiano, G., Ward, R. J., Alvarez-Curto, E. , Marsango, S. and Milligan, G. (2015) The molecular basis of oligomeric organization of the human m3 muscarinic acetylcholine receptor. Molecular Pharmacology, 87(6), pp. 936-953. (doi: 10.1124/mol.114.096925) (PMID:25769304)

Ward, R. J., Pediani, J. D. , Godin, A. G. and Milligan, G. (2015) Regulation of oligomeric organization of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor observed by spatial intensity distribution analysis. Journal of Biological Chemistry, 290(20), pp. 12844-12857. (doi: 10.1074/jbc.M115.644724) (PMID:25825490) (PMCID:PMC4432300)

An, S., Yang, Y., Ward, R., Liu, Y., Guo, X.-X. and Xu, T.-R. (2015) Raf-interactome in tuning the complexity and diversity of Raf function. FEBS Journal, 282(1), pp. 32-53. (doi: 10.1111/febs.13113)

Liu, Y., Yang, Y., Ward, R., An, S., Guo, X.-X., Li, W. and Xu, T.-R. (2015) Biased signalling: the instinctive skill of the cell in the selection of appropriate signalling pathways. Biochemical Journal, 470(2), pp. 155-167. (doi: 10.1042/bj20150358) (PMID:26348905)

2014

Zakrys, L., Ward, R. J., Pediani, J. D. , Godin, A. G., Graham, G. J. and Milligan, G. (2014) Roundabout 1 exists predominantly as a basal dimeric complex and this is unaffected by binding of the ligand Slit2. Biochemical Journal, 461(1), pp. 61-73. (doi: 10.1042/BJ20140190)

Ward, R.J. and Milligan, G. (2014) Structural and biophysical characterisation of G protein-coupled receptor ligand binding using resonance energy transfer and fluorescent labelling techniques. Biochimica et Biophysica Acta: Biomembranes, 1838(1A), pp. 3-14. (doi: 10.1016/j.bbamem.2013.04.007)

Yang, Y., Li, H., Ward, R., Gao, L., Wei, J. and Xu, T. (2014) Novel oxytocin receptor agonists and antagonists: a patent review (2002 – 2013). Expert Opinion on Therapeutic Patents, 24(1), pp. 29-46. (doi: 10.1517/13543776.2014.845168)

2013

Xu, T., Yang, Y., Ward, R., Gao, L. and Liu, Y. (2013) Orexin receptors: multi-functional therapeutic targets for sleeping disorders, eating disorders, drug addiction, cancers and other physiological disorders. Cellular Signalling, 25(12), pp. 2413-2423. (doi: 10.1016/j.cellsig.2013.07.025)

Hudson, B.D. et al. (2013) Defining the molecular basis for the first potent and selective orthosteric agonists of the FFA2 free fatty acid receptor. Journal of Biological Chemistry, 288(24), pp. 17296-17312. (doi: 10.1074/jbc.M113.455337)

Ward, R.W., Xu, T.-X. and Milligan, G. (2013) GPCR oligomerization and receptor trafficking. Methods in Enzymology, 521, pp. 69-90. (doi: 10.1016/B978-0-12-391862-8.00004-1)

2012

Xu, T.-R., Ward, R. J., Pediani, J. D. and Milligan, G. (2012) Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation. Journal of Biological Chemistry, 287, pp. 14937-14949. (doi: 10.1074/jbc.M111.334300)

Laarman, A.J. et al. (2012) Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO Journal, 31(17), pp. 3607-3619. (doi: 10.1038/emboj.2012.212)

2011

Ward, R. J., Pediani, J. D. and Milligan, G. (2011) Heteromultimerization of cannabinoid CB1 receptor and orexin OX1 receptor generates a unique complex in which both protomers are regulated by orexin A. Journal of Biological Chemistry, 286(43), pp. 37414-37428. (doi: 10.1074/jbc.M111.287649)

Xu, T.‑R., Ward, R. J., Pediani, J. D. and Milligan, G. (2011) The orexin OX1 receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands. Biochemical Journal, 439(1), pp. 171-183. (doi: 10.1042/BJ20110230)

Smith, N.J. et al. (2011) Extracellular loop 2 of the free fatty acid receptor 2 mediates allosterism of a phenylacetamide ago-allosteric modulator. Molecular Pharmacology, 80(1), pp. 163-173. (doi: 10.1124/mol.110.070789)

Ward, R. J., Pediani, J. D. and Milligan, G. (2011) Ligand-induced internalisation of the orexin OX1 and cannabinoid CB1 receptors assessed via N-terminal SNAP and CLIP-tagging. British Journal of Pharmacology, 162(6), pp. 1439-1452. (doi: 10.1111/j.1476-5381.2010.01156.x)

Ward, R.J., Alvarez-Curto, E. and Milligan, G. (2011) Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression. Methods in Molecular Biology, 746, pp. 21-37. (doi: 10.1007/978-1-61779-126-0_2)

2010

Alvarez-Curto, E., Ward, R. J., Pediani, J. D. and Milligan, G. (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry, 285(30), pp. 23318-23330. (doi: 10.1074/jbc.M110.122184)

2009

Ward, R. J., Jenkins, L. and Milligan, G. (2009) Selectivity and functional consequences of interactions of family A G protein-coupled receptors with neurochondrin and periplakin. Journal of Neurochemistry, 109(1), pp. 182-192. (doi: 10.1111/j.1471-4159.2009.05918.x)

2004

Ward, R.J. and Milligan, G. (2004) Analysis of function of receptor-G-protein and receptor-RGS fusion proteins. Methods in Molecular Biology, 259, pp. 225-247.

2003

Bahia, D. S., Sartania, N., Ward, R. j., Cavalli, A., Jones, T. L.Z., Druey, K. M. and Milligan, G. (2003) Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity o. Journal of Neurochemistry, 85(5), pp. 1289-1298. (doi: 10.1046/j.1471-4159.2003.01769.x)

This list was generated on Thu Jul 9 10:20:09 2020 BST.
Number of items: 35.

Articles

Stoneman, M. R., Biener, G., Ward, R. J., Pediani, J. D. , Badu, D., Eis, A., Popa, I., Milligan, G. and Raicu, V. (2019) A general method to quantify ligand-driven oligomerization from fluorescence-based images. Nature Methods, 16(6), pp. 493-496. (doi: 10.1038/s41592-019-0408-9) (PMID:31110281)

Milligan, G. , Ward, R. J. and Marsango, S. (2019) GPCR homo-oligomerization. Current Opinion in Cell Biology, 57, pp. 40-47. (doi: 10.1016/j.ceb.2018.10.007) (PMID:30453145)

Müskens, F. M., Ward, R. J., Herkt, D., van de Langemheen, H., Tobin, A. B. , Liskamp, R. M.J. and Milligan, G. (2019) Design, synthesis and evaluation of a diazirine photoaffinity probe for ligand-based receptor capture targeting G protein-coupled receptors. Molecular Pharmacology, 95(2), pp. 196-209. (doi: 10.1124/mol.118.114249) (PMID:30514721) (PMCID:PMC6324650)

Marsango, S., Ward, R. J., Alvarez-Curto, E. and Milligan, G. (2018) Muscarinic receptor oligomerization. Neuropharmacology, 136(Part C), pp. 401-410. (doi: 10.1016/j.neuropharm.2017.11.023) (PMID:29146505) (PMCID:PMC6078712)

Liu, Y. et al. (2018) Assessing the real-time activation of the cannabinoid CB1 receptor and the associated structural changes using a FRET biosensor. International Journal of Biochemistry and Cell Biology, 99, pp. 114-124. (doi: 10.1016/j.biocel.2018.04.003) (PMID:29626639)

Liu, Y. et al. (2018) Visualization of the activation of the histamine H3 receptor (H3R) using novel fluorescence resonance energy transfer biosensors and their potential application to the study of H3R pharmacology. FEBS Journal, 285(12), pp. 2319-2336. (doi: 10.1111/febs.14484) (PMID:29701013)

Pediani, J. D. , Ward, R. J., Marsango, S. and Milligan, G. (2018) Spatial intensity distribution analysis: studies of G Protein-coupled receptor oligomerization. Trends in Pharmacological Sciences, 39(2), pp. 175-186. (doi: 10.1016/j.tips.2017.09.001) (PMID:29032835) (PMCID:PMC5783713)

Ward, R. J., Pediani, J. D. , Harikumar, K. G., Miller, L. J. and Milligan, G. (2017) Spatial intensity distribution analysis quantifies the extent and regulation of homodimerization of the secretin receptor. Biochemical Journal, 474(11), pp. 1879-1895. (doi: 10.1042/BCJ20170184) (PMID:28424368) (PMCID:PMC5442643)

Marsango, S., Caltabiano, G., Jiménez-Rosés, M., Millan, M. J., Pediani, J. D. , Ward, R. J. and Milligan, G. (2017) A molecular basis for selective antagonist destabilization of dopamine D3 receptor quaternary organization. Scientific Reports, 7, 2134. (doi: 10.1038/s41598-017-02249-3) (PMID:28522847) (PMCID:PMC5437050)

Guo, H., An, S., Ward, R. J., Yang, Y., Liu, Y., Guo, X.-X., Hao, Q. and Xu, T.-R. (2017) Methods used to study the oligomeric structure of G protein-coupled receptors. Bioscience Reports, 37(2), (doi: 10.1042/bsr20160547) (PMID:28062602) (PMCID:PMC5398257)

Liu, Y., An, S., Ward, R., Yang, Y., Guo, X., Li, W. and Xu, T.-R. (2016) G protein-coupled receptors as promising cancer targets. Cancer Letters, 376(2), pp. 226-239. (doi: 10.1016/j.canlet.2016.03.031) (PMID:27000991)

Pediani, J. D. , Ward, R. J., Godin, A. G., Marsango, S. and Milligan, G. (2016) Dynamic regulation of quaternary organization of the M1 muscarinic receptor by subtype-selective antagonist drugs. Journal of Biological Chemistry, 291, pp. 13132-13146. (doi: 10.1074/jbc.M115.712562) (PMID:27080256) (PMCID:PMC4933229)

An, S., Yang, Y., Ward, R., Liu, Y., Guo, X.-X. and Xu, T.-R. (2015) A-Raf: A new star of the family of raf kinases. Critical Reviews in Biochemistry and Molecular Biology, 50(6), pp. 520-531. (doi: 10.3109/10409238.2015.1102858) (PMID:26508523)

Liste, M. J., Caltabiano, G., Ward, R. J., Alvarez-Curto, E. , Marsango, S. and Milligan, G. (2015) The molecular basis of oligomeric organization of the human m3 muscarinic acetylcholine receptor. Molecular Pharmacology, 87(6), pp. 936-953. (doi: 10.1124/mol.114.096925) (PMID:25769304)

Ward, R. J., Pediani, J. D. , Godin, A. G. and Milligan, G. (2015) Regulation of oligomeric organization of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor observed by spatial intensity distribution analysis. Journal of Biological Chemistry, 290(20), pp. 12844-12857. (doi: 10.1074/jbc.M115.644724) (PMID:25825490) (PMCID:PMC4432300)

An, S., Yang, Y., Ward, R., Liu, Y., Guo, X.-X. and Xu, T.-R. (2015) Raf-interactome in tuning the complexity and diversity of Raf function. FEBS Journal, 282(1), pp. 32-53. (doi: 10.1111/febs.13113)

Liu, Y., Yang, Y., Ward, R., An, S., Guo, X.-X., Li, W. and Xu, T.-R. (2015) Biased signalling: the instinctive skill of the cell in the selection of appropriate signalling pathways. Biochemical Journal, 470(2), pp. 155-167. (doi: 10.1042/bj20150358) (PMID:26348905)

Zakrys, L., Ward, R. J., Pediani, J. D. , Godin, A. G., Graham, G. J. and Milligan, G. (2014) Roundabout 1 exists predominantly as a basal dimeric complex and this is unaffected by binding of the ligand Slit2. Biochemical Journal, 461(1), pp. 61-73. (doi: 10.1042/BJ20140190)

Ward, R.J. and Milligan, G. (2014) Structural and biophysical characterisation of G protein-coupled receptor ligand binding using resonance energy transfer and fluorescent labelling techniques. Biochimica et Biophysica Acta: Biomembranes, 1838(1A), pp. 3-14. (doi: 10.1016/j.bbamem.2013.04.007)

Yang, Y., Li, H., Ward, R., Gao, L., Wei, J. and Xu, T. (2014) Novel oxytocin receptor agonists and antagonists: a patent review (2002 – 2013). Expert Opinion on Therapeutic Patents, 24(1), pp. 29-46. (doi: 10.1517/13543776.2014.845168)

Xu, T., Yang, Y., Ward, R., Gao, L. and Liu, Y. (2013) Orexin receptors: multi-functional therapeutic targets for sleeping disorders, eating disorders, drug addiction, cancers and other physiological disorders. Cellular Signalling, 25(12), pp. 2413-2423. (doi: 10.1016/j.cellsig.2013.07.025)

Hudson, B.D. et al. (2013) Defining the molecular basis for the first potent and selective orthosteric agonists of the FFA2 free fatty acid receptor. Journal of Biological Chemistry, 288(24), pp. 17296-17312. (doi: 10.1074/jbc.M113.455337)

Ward, R.W., Xu, T.-X. and Milligan, G. (2013) GPCR oligomerization and receptor trafficking. Methods in Enzymology, 521, pp. 69-90. (doi: 10.1016/B978-0-12-391862-8.00004-1)

Xu, T.-R., Ward, R. J., Pediani, J. D. and Milligan, G. (2012) Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation. Journal of Biological Chemistry, 287, pp. 14937-14949. (doi: 10.1074/jbc.M111.334300)

Laarman, A.J. et al. (2012) Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO Journal, 31(17), pp. 3607-3619. (doi: 10.1038/emboj.2012.212)

Ward, R. J., Pediani, J. D. and Milligan, G. (2011) Heteromultimerization of cannabinoid CB1 receptor and orexin OX1 receptor generates a unique complex in which both protomers are regulated by orexin A. Journal of Biological Chemistry, 286(43), pp. 37414-37428. (doi: 10.1074/jbc.M111.287649)

Xu, T.‑R., Ward, R. J., Pediani, J. D. and Milligan, G. (2011) The orexin OX1 receptor exists predominantly as a homodimer in the basal state: potential regulation of receptor organization by both agonist and antagonist ligands. Biochemical Journal, 439(1), pp. 171-183. (doi: 10.1042/BJ20110230)

Smith, N.J. et al. (2011) Extracellular loop 2 of the free fatty acid receptor 2 mediates allosterism of a phenylacetamide ago-allosteric modulator. Molecular Pharmacology, 80(1), pp. 163-173. (doi: 10.1124/mol.110.070789)

Ward, R. J., Pediani, J. D. and Milligan, G. (2011) Ligand-induced internalisation of the orexin OX1 and cannabinoid CB1 receptors assessed via N-terminal SNAP and CLIP-tagging. British Journal of Pharmacology, 162(6), pp. 1439-1452. (doi: 10.1111/j.1476-5381.2010.01156.x)

Ward, R.J., Alvarez-Curto, E. and Milligan, G. (2011) Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression. Methods in Molecular Biology, 746, pp. 21-37. (doi: 10.1007/978-1-61779-126-0_2)

Alvarez-Curto, E., Ward, R. J., Pediani, J. D. and Milligan, G. (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogenous time-resolved FRET. Journal of Biological Chemistry, 285(30), pp. 23318-23330. (doi: 10.1074/jbc.M110.122184)

Ward, R. J., Jenkins, L. and Milligan, G. (2009) Selectivity and functional consequences of interactions of family A G protein-coupled receptors with neurochondrin and periplakin. Journal of Neurochemistry, 109(1), pp. 182-192. (doi: 10.1111/j.1471-4159.2009.05918.x)

Ward, R.J. and Milligan, G. (2004) Analysis of function of receptor-G-protein and receptor-RGS fusion proteins. Methods in Molecular Biology, 259, pp. 225-247.

Bahia, D. S., Sartania, N., Ward, R. j., Cavalli, A., Jones, T. L.Z., Druey, K. M. and Milligan, G. (2003) Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity o. Journal of Neurochemistry, 85(5), pp. 1289-1298. (doi: 10.1046/j.1471-4159.2003.01769.x)

Book Sections

Ward, R. J., Marsango, S., Pediani, J. D. and Milligan, G. (2017) The use of spatial intensity distribution analysis to examine G protein-coupled receptor oligomerization. In: Herrick-Davis, K., Milligan, G. and Di Giovanni, G. (eds.) G-Protein-Coupled Receptor Dimers. Series: Receptors (33). Humana Press: Cham, pp. 15-38. ISBN 9783319601724 (doi:10.1007/978-3-319-60174-8_2)

This list was generated on Thu Jul 9 10:20:09 2020 BST.