Mrs Marie Pringle

  • Technical Specialist/Lab Manager (Institute of Molecular Cell & Systems Biology Administration)

Biography

Key Skills 

Grant Funded

Development of Novel techniques within our Lab/Institute/University:

  • CRISPR Technology
  • RIBOSOME Profiling

High level of expertise (over 20 years) in a range of techniques: 

  • Molecular Biology: Cloning, site-directed mutagenesis, PCR, RT-PCR, QPCR, RNA/DNA/Protein extraction from cells/tissues, Western Blotting, Southern Blotting, Northern Blotting, In-Vibo Transgenic models 
  • Biochemical: In-vitro transcriptions, In-Vitro translations, Immunoprecipitations, Protein expression & Purification 
  • Cell Culture: Maintenance of variety of cell lines, Generation of stable cell lines, Generation of Knock out cell lines via CRISPR, 3D Cell Culture 
  • Lab Management: Health and safety, inductions, maintenance of lab stock databses, delegated control of budgetary spending/ordering lab materials, equipment maintenanece, liason internal/external collaborators
  • Management technical staff: daily supervision of junior technical staff P & DR reviewer, training students/staff techniques, equipment use 
  • Health and safety committee member 
  • Organsing/presenting at technical workshops 
  • T4 web design course completion 

 


Research interests

I have worked in 3 main research areas in the past 20 years. These are Protein Folding and Assembly in the Endoplasmic Reticulum (current-past 10 years), Mammary Gland Development & Breast Cancer (8 years) and Transgenic Mouse models in Leukaemia Research (3 years). I enjoy both the technical and management duties that I fulfil. I have particular interest in developing new technologies and using my technical expertise to troubleshoot any arising issues before knowledge transfer to others within the lab or wider academic community. 


Publications

List by: Type | Date

Jump to: 2019 | 2017 | 2016 | 2015 | 2013 | 2010
Number of items: 9.

2019

Oka, O. B.V. , van Lith, M., Rudolf, J., Tungkum, W., Pringle, M.-A. and Bulleid, N. J. (2019) ERp18 regulates the activation of ATF6α during the unfolded protein response. EMBO Journal, 2019, e100990. (doi: 10.15252/embj.2018100990) (PMID:31209066)

2017

Robinson, P. J. , Pringle, M. A. , Woolhead, C. A. and Bulleid, N. J. (2017) Folding of a single domain protein entering the endoplasmic reticulum precedes disulfide formation. Journal of Biological Chemistry, 292(17), pp. 6978-6986. (doi: 10.1074/jbc.M117.780742) (PMID:28298446) (PMCID:PMC5409466)

Poet, G. J., Oka, O. B.V. , Van Lith, M., Cao, Z., Robinson, P. J. , Pringle, M. A. , Arnér, E. S.J. and Bulleid, N. (2017) Cytosolic thioredoxin reductase 1 is required for correct disulfide formation in the ER. EMBO Journal, 36(5), pp. 693-702. (doi: 10.15252/embj.201695336) (PMID:28093500) (PMCID:PMC5331760)

2016

Cao, Z., Van Lith, M., Mitchell, L. J., Pringle, M. A. , Inaba, K. and Bulleid, N. J. (2016) The membrane topology of vitamin K epoxide reductase is conserved between human isoforms and the bacterial enzyme. Biochemical Journal, 473(7), pp. 851-858. (doi: 10.1042/bj20151223) (PMID:26772871)

2015

Iglesias, J. M. et al. (2015) Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium. PLoS ONE, 10(3), e0119718. (doi: 10.1371/journal.pone.0119718) (PMID:25803307) (PMCID:PMC4372349)

2013

Rudolf, J., Pringle, M. and Bulleid, N. (2013) Proteolytic processing of QSOX1A ensures efficient secretion of a potent disulfide catalyst. Biochemical Journal, 2013(454), pp. 181-190. (doi: 10.1042/BJ20130360)

Oka, O. B. V. , Pringle, M. A. , Schopp, I. M., Braakman, I. and Bulleid, N. J. (2013) ERdj5 is the ER reductase that catalyzes the removal of non-native disulfides and correct folding of the LDL receptor. Molecular Cell, 50(6), pp. 793-804. (doi: 10.1016/j.molcel.2013.05.014)

2010

Blyth, K. , Vaillant, F., Jenkins, A., McDonald, L., Pringle, M. A. , Huser, C. , Stein, T., Neil, J. and Cameron, E. R. (2010) Runx2 in normal tissues and cancer cells: A developing story. Blood Cells, Molecules, and Diseases, 45(2), pp. 117-123. (doi: 10.1016/j.bcmd.2010.05.007)

Huser, C.A., Pringle, M.A., Heath, V.J., Bell, A.K., Kendrick, H., Smalley, M.J., Crighton, D., Ryan, K.M. , Gusterson, B.A. and Stein, T. (2010) TSC-22D1 isoforms have opposing roles in mammary epithelial cell survival. Cell Death and Differentiation, 17(2), pp. 304-315. (doi: 10.1038/cdd.2009.126)

This list was generated on Wed Jul 8 23:51:59 2020 BST.
Jump to: Articles
Number of items: 9.

Articles

Oka, O. B.V. , van Lith, M., Rudolf, J., Tungkum, W., Pringle, M.-A. and Bulleid, N. J. (2019) ERp18 regulates the activation of ATF6α during the unfolded protein response. EMBO Journal, 2019, e100990. (doi: 10.15252/embj.2018100990) (PMID:31209066)

Robinson, P. J. , Pringle, M. A. , Woolhead, C. A. and Bulleid, N. J. (2017) Folding of a single domain protein entering the endoplasmic reticulum precedes disulfide formation. Journal of Biological Chemistry, 292(17), pp. 6978-6986. (doi: 10.1074/jbc.M117.780742) (PMID:28298446) (PMCID:PMC5409466)

Poet, G. J., Oka, O. B.V. , Van Lith, M., Cao, Z., Robinson, P. J. , Pringle, M. A. , Arnér, E. S.J. and Bulleid, N. (2017) Cytosolic thioredoxin reductase 1 is required for correct disulfide formation in the ER. EMBO Journal, 36(5), pp. 693-702. (doi: 10.15252/embj.201695336) (PMID:28093500) (PMCID:PMC5331760)

Cao, Z., Van Lith, M., Mitchell, L. J., Pringle, M. A. , Inaba, K. and Bulleid, N. J. (2016) The membrane topology of vitamin K epoxide reductase is conserved between human isoforms and the bacterial enzyme. Biochemical Journal, 473(7), pp. 851-858. (doi: 10.1042/bj20151223) (PMID:26772871)

Iglesias, J. M. et al. (2015) Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium. PLoS ONE, 10(3), e0119718. (doi: 10.1371/journal.pone.0119718) (PMID:25803307) (PMCID:PMC4372349)

Rudolf, J., Pringle, M. and Bulleid, N. (2013) Proteolytic processing of QSOX1A ensures efficient secretion of a potent disulfide catalyst. Biochemical Journal, 2013(454), pp. 181-190. (doi: 10.1042/BJ20130360)

Oka, O. B. V. , Pringle, M. A. , Schopp, I. M., Braakman, I. and Bulleid, N. J. (2013) ERdj5 is the ER reductase that catalyzes the removal of non-native disulfides and correct folding of the LDL receptor. Molecular Cell, 50(6), pp. 793-804. (doi: 10.1016/j.molcel.2013.05.014)

Blyth, K. , Vaillant, F., Jenkins, A., McDonald, L., Pringle, M. A. , Huser, C. , Stein, T., Neil, J. and Cameron, E. R. (2010) Runx2 in normal tissues and cancer cells: A developing story. Blood Cells, Molecules, and Diseases, 45(2), pp. 117-123. (doi: 10.1016/j.bcmd.2010.05.007)

Huser, C.A., Pringle, M.A., Heath, V.J., Bell, A.K., Kendrick, H., Smalley, M.J., Crighton, D., Ryan, K.M. , Gusterson, B.A. and Stein, T. (2010) TSC-22D1 isoforms have opposing roles in mammary epithelial cell survival. Cell Death and Differentiation, 17(2), pp. 304-315. (doi: 10.1038/cdd.2009.126)

This list was generated on Wed Jul 8 23:51:59 2020 BST.