Professor Gwyn Gould

  • Affiliate (Institute of Molecular Cell & Systems Biology)

Biography

Gwyn was born and raised in South Wales and read Biochemistry at the University of Southampton, graduating in 1983. He undertook his PhD in membrane biophysics with Tony Lee, studying aspects of the kinetics of the Ca2+ ATPase of sarcoplasmic reticulum, and the impact of phospholipid geometry on the function of this pump. In 1986, he moved to Dartmouth Medical School to work with Gus Lienhard on glucose transport, supported by a fellowship from the Juvenile Diabetes Foundation International. This began a 30-year career studying aspects of glucose transport.  After visiting scientist posts at Washington University School of Medicine St. Louis and University of California Los Angeles, he moved to Glasgow in 1989. During his time at Glasgow, Gwyn has held a Lister Institute of Preventive Medicine Research Fellowship and a Wellcome Trust Research Leave post. He has been Professor of Cell Biology since 2005. Research in his lab is presently supported by Diabetes UK, The British Heart Foundation and the Novo Nordisk Research Foundation.


Research interests

Insulin stimulates glucose transport into fat and muscle by regulating delivery of the facilitative glucose transporter GLUT4 from an intracellular store to the cell surface. Upon binding its receptor, insulin initiates a signalling cascade that culminates in changes in the trafficking itinerary of GLUT4, releasing it from its intracellular store and delivering it to the cell surface. Individuals with insulin resistance and Type-2 diabetes exhibit defective insulin-stimulated GLUT4 translocation and consequently much effort has gone into defining the trafficking of GLUT4 in adipocytes, cardiomyocytes and muscle cells. Our work in this area seek to define the mechanisms which control GLUT4 sorting/trafficking, the mechanism by which GLUT4 is delivered to the cell surface, and the processes which control its spatial distribution on the cell surface.

Present projects include:
•    Understanding the interface of insulin signalling and membrane trafficking: defining how tyrosine phosphorylation of key trafficking machinery impacts GLUT4 delivery to the cell surface and the spatial relationship of insulin signalling and trafficking hubs.
•    Examination of the relationship between insulin sensitivity and SNARE protein phosphorylation in human populations.
•    Using unorthodox kinases to regulate GLUT4 trafficking in insulin-sensitive tissues as an alternative therapeutic target.
•    Defining the mechanisms by which insulin regulates the cell surface distribution of GLUT4 using cell biological, genetic and super-resolution approaches.
•    Developing the use of induced pluripotent stem cell-derived cardiomyocytes as model systems for the study of diabetic cardiomyopathy
•    Using ontogenetic approaches to modulate glucose transport in cardiomyocytes and myotubes.
•    Defining the role of cholesterol in insulin sensitive cells and tissues.

Research interests - further information

For the general public


Publications

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Number of items: 68.

2020

Morris, S. et al. (2020) Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes. PeerJ, 8, e8751. (doi: 10.7717/peerj.8751) (PMID:32185116) (PMCID:PMC7060922)

2019

Gould, G. , Bowman, P. and Smith, G. (2019) Cardiac SNARE expression in health and disease. Frontiers in Endocrinology, 10, 881. (doi: 10.3389/fendo.2019.00881) (PMID:31920989) (PMCID:PMC6930865)

Bowman, P. R.T., Smith, G. L. and Gould, G. W. (2019) GLUT4 expression and glucose transport in human induced pluripotent stem cell-derived cardiomyocytes. PLoS ONE, 14(7), e0217885. (doi: 10.1371/journal.pone.0217885) (PMID:31920989) (PMCID:PMC6930865)

Sadler, J. B.A., Lambert, C. A., Welburn, C. R., Adamson, I. S., Kioumourtzoglou, D., Chi, N.-W., Gould, G. W. and Bryant, N. J. (2019) The deubiquitinating enzyme USP25 binds tankyrase and regulates trafficking of the facilitative glucose transporter GLUT4 in adipocytes. Scientific Reports, 9(1), 4710. (doi: 10.1038/s41598-019-40596-5) (PMID:30886164) (PMCID:PMC6423145)

Rezig, I. M., Bremner, S. K., Bhutta, M. S., Salt, I. P. , Gould, G. W. and McInerny, C. J. (2019) Genetic and cytological methods to study ESCRT cell cycle function in fission yeast. In: Culetto, E. and Legouis, R. (eds.) The ESCRT Complexes: Methods and Protocols. Series: Methods in molecular biology (1998). Humana Press: New York, pp. 239-250. ISBN 9781493994915 (doi:10.1007/978-1-4939-9492-2_18)

2018

Wood, R. A., Barbour, M. J., Gould, G. W. , Cunningham, M. R. and Plevin, R. J. (2018) Conflicting evidence for the role of JNK as a target in breast cancer cell proliferation: comparisons between pharmacological inhibition and selective shRNA knockdown approaches. Pharmacology Research and Perspectives, 6(1), e00376. (doi: 10.1002/prp2.376)

Kioumourtzoglou, D., Gould, G. W. and Bryant, N. J. (2018) Proximity ligation assay to study the GLUT4 membrane trafficking machinery. In: Lindkvist-Petersson, K. and Hansen, J. S. (eds.) Glucose Transport: Methods and Protocols. Series: Methods in molecular biology (1713). Humana Press: New York, pp. 217-227. ISBN 9781493975068 (doi:10.1007/978-1-4939-7507-5_16)

2017

Boehm, C. M., Obado, S., Gadelha, C., Kaupisch, A., Manna, P. T., Gould, G. W. , Munson, M., Chait, B. T., Rout, M. P. and Field, M. C. (2017) The trypanosome exocyst: a conserved structure revealing a new role in endocytosis. PLoS Pathogens, 13(1), e1006063. (doi: 10.1371/journal.ppat.1006063) (PMID:28114397) (PMCID:PMC5256885)

Laidlaw, K. M.E., Livingstone, R. , Al-Tobi, M. , Bryant, N. J. and Gould, G. W. (2017) SNARE phosphorylation: a control mechanism for insulin-stimulated glucose transport and other regulated exocytic events. Biochemical Society Transactions, 45(6), pp. 1271-1277. (doi: 10.1042/BST20170202) (PMID:29101310)

2016

Heathcote, H. R., Mancini, S. J., Strembitska, A., Jamal, K., Reihill, J. A., Palmer, T. M., Gould, G. W. and Salt, I. P. (2016) Protein kinase C phosphorylates AMP-activated protein kinase α1 Ser487. Biochemical Journal, 473(24), pp. 4681-4697. (doi: 10.1042/BCJ20160211) (PMID:27784766) (PMCID:PMC5147050)

Gould, G. W. (2016) Animal cell cytokinesis: the role of dynamic changes in the plasma membrane proteome and lipidome. Seminars in Cell and Developmental Biology, 53, pp. 64-73. (doi: 10.1016/j.semcdb.2015.12.012) (PMID:26721337)

2015

Kioumourtzoglou, D., Pryor, P. R., Gould, G. W. and Bryant, N. J. (2015) Alternate routes to the cell surface underpin insulin-regulated membrane trafficking of GLUT4. Journal of Cell Science, 128, pp. 2423-2429. (doi: 10.1242/jcs.166561) (PMID:26071524) (PMCID:PMC4510850)

Sadler, J. B.A., Bryant, N. J. and Gould, G. W. (2015) Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking. Molecular Biology of the Cell, 26(3), pp. 530-536. (doi: 10.1091/mbc.E14-09-1368) (PMID:25501368)

Sadler, J. B.A., Roccisana, J., Virolainen, M., Bryant, N. J. and Gould, G. W. (2015) mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes. Communicative and Integrative Biology, 8(3), e1026494. (doi: 10.1080/19420889.2015.1026494) (PMID:26479872) (PMCID:PMC4594494)

2014

Bhutta, M. S., Roy, B., Gould, G. W. and McInerny, C. J. (2014) A complex network of interactions between mitotic kinases, phosphatases and ESCRT proteins regulates septation and membrane trafficking in S. pombe. PLoS ONE, 9(10), e111789. (doi: 10.1371/journal.pone.0111789) (PMID:25356547) (PMCID:PMC4214795)

Kioumourtzoglou, D., Gould, G. W. and Bryant, N. J. (2014) Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism. Molecular and Cellular Biology, 34(7), pp. 1271-1279. (doi: 10.1128/MCB.01203-13)

Bhutta, M. S., McInerny, C. J. and Gould, G. W. (2014) ESCRT function in cytokinesis: location, dynamics and regulation by mitotic kinases. International Journal of Molecular Sciences, 15(12), pp. 21723-21739. (doi: 10.3390/ijms151221723) (PMID:25429432) (PMCID:PMC4284674)

Kioumourtzoglou, D., Sadler, J. B.A., Black, H. L., Berends, R., Wellburn, C., Bryant, N. J. and Gould, G. W. (2014) Studies of the regulated assembly of SNARE complexes in adipocytes. Biochemical Society Transactions, 42(5), pp. 1396-1400. (doi: 10.1042/BST20140114)

2013

Neto, H., Kaupisch, A., Collins, L.L. and Gould, G.W. (2013) Syntaxin 16 is a master recruitment factor for cytokinesis. Molecular Biology of the Cell, 24(23), pp. 3663-3674. (doi: 10.1091/mbc.E13-06-0302) (PMID:24109596) (PMCID:PMC3842993)

Roccisana, J., Sadler, J.B.A., Bryant, N.J. and Gould, G.W. (2013) Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45. Molecular Biology of the Cell, 24(15), pp. 2389-97. (doi: 10.1091/mbc.E13-01-0011)

Sadler, J.B., Bryant, N.J., Gould, G.W. and Welburn, C.R. (2013) Posttranslational modifications of GLUT4 affect its subcellular localization and translocation. International Journal of Molecular Sciences, 14(5), pp. 9963-9978. (doi: 10.3390/ijms14059963)

2012

Cunningham, M. R., McIntosh, K. A., Pediani, J. D. , Robben, J., Cooke, A. E., Nilsson, M., Gould, G. W. , Mundell, S., Milligan, G. and Plevin, R. (2012) Novel role for proteinase-activated receptor 2 (PAR2) in membrane trafficking of proteinase-activated receptor 4 (PAR4). Journal of Biological Chemistry, 287(20), pp. 16656-16669. (doi: 10.1074/jbc.M111.315911)

Collins, L.L., Simon, G., Matheson, J., Wu, C., Miller, M.C., Otani, T., Yu, X., Hayashi, S., Prekeris, R. and Gould, G.W. (2012) Rab11-FIP3 is a cell cycle-regulated phosphoprotein. BMC Cell Biology, 13(1), p. 4. (doi: 10.1186/1471-2121-13-4) (PMID:22401586) (PMCID:PMC3310825)

2011

Aran, V., Bryant, N.J. and Gould, G.W. (2011) Tyrosine phosphorylation of Munc18c on residue 521 abrogates binding to Syntaxin 4. BMC Biochemistry, 12(19), (doi: 10.1186/1471-2091-12-19)

Bryant, N.J. and Gould, G.W. (2011) SNARE proteins underpin insulin-regulated GLUT4 traffic. Traffic, 12(6), pp. 657-664. (doi: 10.1111/j.1600-0854.2011.01163.x)

Gould, G.W. (2011) IKK : A Kinase at the Intersection of Signaling and Membrane Traffic. Science Signaling, 4(177), pe30. (doi: 10.1126/scisignal.2002186)

Neto, H., Collins, L.L. and Gould, G.W. (2011) Vesicle trafficking and membrane remodelling in cytokinesis. Biochemical Journal, 437(1), pp. 13-24. (doi: 10.1042/BJ20110153)

Neto, H. and Gould, G.W. (2011) The regulation of abscission by multi-protein complexes. Journal of Cell Science, 124(19), pp. 3199-3207. (doi: 10.1242/jcs.083949)

2009

Gould, G.W. and Lippincott-Schwartz, J. (2009) New roles for endosomes: from vesicular carriers to multi-purpose platforms. Nature Reviews Molecular Cell Biology, 10(4), pp. 287-292. (doi: 10.1038/nrm2652)

Aran-Ponte, V., Brandie, F.M., Boyd, A.R., Kantidakis, T., Rideout, E.J., Kelly, S.M., Gould, G.W. and Bryant, N.J. (2009) Characterization of two distinct binding modes between syntaxin 4 and Munc18c. Biochemical Journal, 419(Pt3), pp. 655-660. (doi: 10.1042/BJ20082293) (PMID:19193195)

2008

Simon, G.C., Schonteich, E., Wu, C.C., Piekny, A., Ekiert, D., Yu, X., Gould, G.W. , Glotzer, M. and Prekeris, R. (2008) Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis. EMBO Journal, 27(13), pp. 1791-1803. (doi: 10.1038/emboj.2008.112) (PMID:18511905) (PMCID:PMC2486418)

Brandie, F.M., Aran-Ponte, V., Verma, A., McNew, J.A., Bryant, N.J. and Gould, G.W. (2008) Negative regulation of syntaxin4/SNAP-23/VAMP2-mediated membrane fusion by Munc18c In Vitro. PLoS ONE, 3(12), e4074. (doi: 10.1371/journal.pone.0004074) (PMID:19116655) (PMCID:PMC2605266)

Prekeris, R. and Gould, G.W. (2008) Breaking up is hard to do - membrane traffic in cytokinesis. Journal of Cell Science, 121(10), pp. 1569-1576. (doi: 10.1242/jcs.018770)

2007

Yu, X., Prekeris, R. and Gould, G. (2007) Role of endosomal Rab GTPases in cytokinesis. European Journal of Cell Biology, 86, pp. 25-35. (doi: 10.1016/j.ejcb.2006.10.002)

2006

Choudhury, A., Marks, D., Proctor, K., Gould, G. and Pagano, R. (2006) Regulation of caveolar endocytosis by syntaxin 6-dependent delivery of membrane components to the cell surface. Nature Cell Biology, 8, 317-U6. (doi: 10.1038/ncb1380)

Clarke, M., Ewart, M.A., Santy, L.C., Prekeris, R. and Gould, G.W. (2006) ACRP30 is secreted from 3T3-L1 adipocytes via a Rab11-dependent pathway. Biochemical and Biophysical Research Communications, 342, pp. 1361-1367. (doi: 10.1016/j.bbrc.2006.02.102) (PMID:16516854)

Proctor, K., Miller, S., Bryant, N. and Gould, G. (2006) Syntaxin 16 controls the intracellular sequestration of GLUT4 in 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications, 347, pp. 433-438. (doi: 10.1016/j.bbrc.2006.06.135)

2005

Collison, M., James, D., Graham, D., Holman, G., Connell, J., Dominiczak, A.F., Gould, G. and Salt, I. (2005) Reduced insulin-stimulated GLUT4 bioavailability in stroke-prone spontaneously hypertensive rats. Diabetologia, 48(3), pp. 539-546. (doi: 10.1007/s00125-005-1674-x)

Ewart, M.-A., Clarke, M., Kane, S., Chamberlain, L.H. and Gould, G.W. (2005) Evidence for a role of the exocyst in insulin-stimulated Glut4 trafficking in 3T3-L1 adipocytes. Journal of Biological Chemistry, 280, pp. 3812-3816. (doi: 10.1074/jbc.M409928200) (PMID:15550383)

Fielding, A.B., Schonteich, E., Matheson, J., Wilson, G., Yu, X.Z., Hickson, G.R.X., Srivastava, S., Baldwin, S.A., Prekeris, R. and Gould, G.W. (2005) Rab11-FIP3 and FIP4 interact with Arf6 and the Exocyst to control membrane traffic in cytokinesis. EMBO Journal, 24, pp. 3389-3399. (doi: 10.1038/sj.emboj.7600803) (PMID:16148947) (PMCID:PMC1276165)

Matheson, J., Yu, X., Fielding, A. and Gould, G. (2005) Membrane traffic in cytokinesis. Biochemical Society Transactions, 33, pp. 1290-1294.

McGettigan, J. et al. (2005) Insect renal tubules constitute a cell-autonomous immune system that protects the organism against bacterial infection. Insect Biochemistry and Molecular Biology, 35, pp. 741-754. (doi: 10.1016/j.ibmb.2005.02.017) (PMID:15894191)

Salaun, C., Gould, G. and Chamberlain, L. (2005) Lipid raft association of SNARE proteins regulates exocytosis in PC12 cells. Journal of Biological Chemistry, 280, pp. 19449-19453. (doi: 10.1074/jbc.M501923200)

Salaun, C., Gould, G. and Chamberlain, L. (2005) The SNARE proteins SNAP-25 and SNAP-23 display different affinities for lipid rafts in PC12 cells - Regulation by distinct cysteine-rich domains. Journal of Biological Chemistry, 280, pp. 1236-1240. (doi: 10.1074/jbc.M410674200)

Tsuboi, T., Ravier, M.A., Xie, H., Ewart, M.-A., Gould, G.W. , Baldwin, S.A. and Rutter, G.A. (2005) Mammalian exocyst complex is required for the docking step of insulin vesicle exocytosis. Journal of Biological Chemistry, 280(27), pp. 25565-25570. (doi: 10.1074/jbc.M501674200)

Wilson, G., Fielding, A., Simon, G., Yu, X., Andrews, P., Hames, R., Frey, A., Peden, A., Gould, G. and Prekeris, R. (2005) The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis. Molecular Biology of the Cell, 16, pp. 849-860.

2004

Litherland, G., Hajduch, E., Gould, G. and Hundal, H. (2004) Fructose transport and metabolism in adipose tissue of Zucker rats: Diminished GLUT5 activity during obesity and insulin resistance. Molecular and Cellular Biochemistry, 261, pp. 23-33.

2003

Hickson, G., Matheson, J., Riggs, B., Maier, V., Fielding, A., Prekeris, R., Sullivan, W., Barr, F. and Gould, G. (2003) Arfophilins are dual Arf/Rab 11 binding proteins that regulate recycling endosome distribution and are related to Drosophila nuclear fallout. Molecular Biology of the Cell, 14, pp. 2908-2920. (doi: 10.1091/mbc.E03-03-0160)

Morrow, V., Foufelle, F., Connell, J., Petrie, J. , Gould, G. and Salt, I. (2003) Direct activation of AMP-activated protein kinase stimulates nitric-oxide synthesis in human aortic endothelial cells. Journal of Biological Chemistry, 278(34), pp. 31629-31639. (doi: 10.1074/jbc.M212831200)

Perera, H., Clarke, M., Morris, N., Hong, W., Chamberlain, L. and Gould, G. (2003) Syntaxin 6 regulates Glut4 trafficking in 3T3-L1 adipocytes. Molecular Biology of the Cell, 14, pp. 2946-2958. (doi: 10.1091/mbc.E02-11-0722)

Perry, C., Palmer, T., Cleland, S., Morton, I., Salt, I. , Petrie, J. , Gould, G. and Connell, J. (2003) Decreased insulin sensitivity during dietary sodium restriction is not mediated by effects of angiotensin II on insulin action. Clinical Science, 105(2), pp. 187-194. (doi: 10.1042/CS20020320)

Riggs, B., Rothwell, W., Mische, S., Hickson, G., Matheson, J., Hays, T., Gould, G. and Sullivan, W. (2003) Actin cytoskeleton remodeling during early Drosophila furrow formation requires recycling endosomal components Nuclear-fallout and RAb11. Journal of Cell Biology, 163, pp. 143-154. (doi: 10.1083/jcb.200305115)

2002

Chamberlain, L. and Gould, G. (2002) The vesicle- and target-SNARE proteins that mediate Glut4 vesicle fusion are localized in detergent-insoluble lipid rafts present on distinct intracellular membranes. Journal of Biological Chemistry, 277, pp. 49750-49754. (doi: 10.1074/jbc.M206936200)

Kelly, C.J.G., Lyall, H., Petrie, J.R. , Gould, G.W. , Connell, J.M.C., Rumley, A., Lowe, G.D.O. and Sattar, N. (2002) A specific elevation in tissue plasminogen activator antigen in women with polycystic ovarian syndrome. Journal of Clinical Endocrinology and Metabolism, 87(7), pp. 3287-3290. (doi: 10.1210/jc.87.7.3287)

Kelly, C., Speirs, A., Gould, G., Petrie, J. , Lyall, H. and Connell, J. (2002) Altered vascular function in young women with polycystic ovary syndrome. Journal of Clinical Endocrinology and Metabolism, 87(2), pp. 742-746. (doi: 10.1210/jc.87.2.742)

2001

Chamberlain, L., Burgoyne, R. and Gould, G. (2001) SNARE proteins are highly enriched in lipid rafts in PC12 cells: Implications for the spatial control of exocytosis. Proceedings of the National Academy of Sciences of the United States of America, 98(10), pp. 5619-5624. (doi: 10.1073/pnas.091502398)

Chamberlain, L., Graham, M., Kane, S., Jackson, J., Maier, V., Burgoyne, R. and Gould, G. (2001) The synaptic vesicle protein, cysteine-string protein, is associated with the plasma membrane in 3T3-L1 adipocytes and interacts with syntaxin 4. Journal of Cell Science, 114, pp. 445-455.

James, D.J., Cairns, F., Salt, I.P. , Murphy, G.J., Dominiczak, A.F., Connell, J.M.C. and Gould, G.W. (2001) Skeletal muscle of stroke-prone spontaneously hypertensive rats exhibits reduced insulin-stimulated glucose transport and elevated levels of caveolin and flotillin. Diabetes, 50(9), pp. 2148-2156. (doi: 10.2337/diabetes.50.9.2148)

Kelly, C., Lyall, H., Petrie, J. , Gould, G., Connell, J. and Sattar, N. (2001) Low grade chronic inflammation in women with polycystic ovarian syndrome. Journal of Clinical Endocrinology and Metabolism, 86(6), pp. 2453-2455. (doi: 10.1210/jc.86.6.2453)

MacKenzie, C.J., Wakefield, J.M., Cairns, F., Dominiczak, A.F. and Gould, G.W. (2001) Regulation of glucose transport in aortic smooth muscle cells by cAMP and cGMP. Biochemical Journal, 353, pp. 513-519.

Petrie, J. , Collison, M., Connell, J., Gould, G. and Dominiczak, A. (2001) CD36 deficiency and insulin resistance. Lancet, 358(9277), pp. 242-243. (doi: 10.1016/S0140-6736(01)05428-9)

2000

Collison, M., Campbell, I., Salt, I. , Dominiczak, A. , Connell, J., Lyall, H. and Gould, G. (2000) Sex hormones induce insulin resistance in 3T3-L1 adipocytes by reducing cellular content of IRS proteins. Diabetologia, 43(11), pp. 1374-1380. (doi: 10.1007/s001250051541)

Salt, I. , Connell, J. and Gould, G. (2000) 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes. Diabetes, 49(10), pp. 1649-1656. (doi: 10.2337/diabetes.49.10.1649)

1997

Thomson, F. J. , Jess, T. J., Moyes, C., Plevin, R. and Gould, G. (1997) Characterization of the intracellular signalling pathways that underlie growth-factor-stimulated glucose transport in Xenopus oocytes: evidence for ras- and rho-dependent pathways of phosphatidylinositol 3-kinase activation. Biochemical Journal, 325(3), pp. 637-643. (PMID:9271083)

1996

Jess, T. J., Belham, C. M., Thomson, F. J. , Scott, P. H., Plevin, R. J. and Gould, G. (1996) Phosphatidylinositol 3′-kinase, but not p70 ribosomal S6 kinase, is involved in membrane protein recycling: Wortmannin inhibits glucose transport and downregulates cell-surface transferrin receptor numbers independently of any effect on fluid-phase endocytosis in fibroblasts. Cellular Signalling, 8(4), pp. 297-304. (doi: 10.1016/0898-6568(96)00054-X) (PMID:8842531)

Livingstone, C. et al. (1996) Hormonal regulation of the insulin-responsive glucose transporter, GLUT4: some recent advances. Proceedings of the Nutrition Society, 55(1B), pp. 179-190. (doi: 10.1079/PNS19960019) (PMID:8832790)

Thomson, F. j. , Moyes, C., Scott, P. H., Plevin, R. and Gould, G. W. (1996) Lysophosphatidic acid stimulates glucose transport in Xenopus oocytes via a phosphatidylinositol 3'-kinase with distinct properties. Biochemical Journal, 316(1), pp. 161-166. (PMID:8645200) (PMCID:PMC1217317)

1995

Gould, G. W. , Cuenda, A., Thomson, F. J. and Cohen, P. (1995) The activation of distinct mitogen-activated protein kinase cascades is required for the stimulation of 2-deoxyglucose uptake by interleukin-1 and insulin-like growth factor-1 in KB cells. Biochemical Journal, 311(3), pp. 735-738. (PMID:7487926)

This list was generated on Thu Nov 26 08:55:46 2020 GMT.
Jump to: Articles | Book Sections
Number of items: 68.

Articles

Morris, S. et al. (2020) Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes. PeerJ, 8, e8751. (doi: 10.7717/peerj.8751) (PMID:32185116) (PMCID:PMC7060922)

Gould, G. , Bowman, P. and Smith, G. (2019) Cardiac SNARE expression in health and disease. Frontiers in Endocrinology, 10, 881. (doi: 10.3389/fendo.2019.00881) (PMID:31920989) (PMCID:PMC6930865)

Bowman, P. R.T., Smith, G. L. and Gould, G. W. (2019) GLUT4 expression and glucose transport in human induced pluripotent stem cell-derived cardiomyocytes. PLoS ONE, 14(7), e0217885. (doi: 10.1371/journal.pone.0217885) (PMID:31920989) (PMCID:PMC6930865)

Sadler, J. B.A., Lambert, C. A., Welburn, C. R., Adamson, I. S., Kioumourtzoglou, D., Chi, N.-W., Gould, G. W. and Bryant, N. J. (2019) The deubiquitinating enzyme USP25 binds tankyrase and regulates trafficking of the facilitative glucose transporter GLUT4 in adipocytes. Scientific Reports, 9(1), 4710. (doi: 10.1038/s41598-019-40596-5) (PMID:30886164) (PMCID:PMC6423145)

Wood, R. A., Barbour, M. J., Gould, G. W. , Cunningham, M. R. and Plevin, R. J. (2018) Conflicting evidence for the role of JNK as a target in breast cancer cell proliferation: comparisons between pharmacological inhibition and selective shRNA knockdown approaches. Pharmacology Research and Perspectives, 6(1), e00376. (doi: 10.1002/prp2.376)

Boehm, C. M., Obado, S., Gadelha, C., Kaupisch, A., Manna, P. T., Gould, G. W. , Munson, M., Chait, B. T., Rout, M. P. and Field, M. C. (2017) The trypanosome exocyst: a conserved structure revealing a new role in endocytosis. PLoS Pathogens, 13(1), e1006063. (doi: 10.1371/journal.ppat.1006063) (PMID:28114397) (PMCID:PMC5256885)

Laidlaw, K. M.E., Livingstone, R. , Al-Tobi, M. , Bryant, N. J. and Gould, G. W. (2017) SNARE phosphorylation: a control mechanism for insulin-stimulated glucose transport and other regulated exocytic events. Biochemical Society Transactions, 45(6), pp. 1271-1277. (doi: 10.1042/BST20170202) (PMID:29101310)

Heathcote, H. R., Mancini, S. J., Strembitska, A., Jamal, K., Reihill, J. A., Palmer, T. M., Gould, G. W. and Salt, I. P. (2016) Protein kinase C phosphorylates AMP-activated protein kinase α1 Ser487. Biochemical Journal, 473(24), pp. 4681-4697. (doi: 10.1042/BCJ20160211) (PMID:27784766) (PMCID:PMC5147050)

Gould, G. W. (2016) Animal cell cytokinesis: the role of dynamic changes in the plasma membrane proteome and lipidome. Seminars in Cell and Developmental Biology, 53, pp. 64-73. (doi: 10.1016/j.semcdb.2015.12.012) (PMID:26721337)

Kioumourtzoglou, D., Pryor, P. R., Gould, G. W. and Bryant, N. J. (2015) Alternate routes to the cell surface underpin insulin-regulated membrane trafficking of GLUT4. Journal of Cell Science, 128, pp. 2423-2429. (doi: 10.1242/jcs.166561) (PMID:26071524) (PMCID:PMC4510850)

Sadler, J. B.A., Bryant, N. J. and Gould, G. W. (2015) Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking. Molecular Biology of the Cell, 26(3), pp. 530-536. (doi: 10.1091/mbc.E14-09-1368) (PMID:25501368)

Sadler, J. B.A., Roccisana, J., Virolainen, M., Bryant, N. J. and Gould, G. W. (2015) mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes. Communicative and Integrative Biology, 8(3), e1026494. (doi: 10.1080/19420889.2015.1026494) (PMID:26479872) (PMCID:PMC4594494)

Bhutta, M. S., Roy, B., Gould, G. W. and McInerny, C. J. (2014) A complex network of interactions between mitotic kinases, phosphatases and ESCRT proteins regulates septation and membrane trafficking in S. pombe. PLoS ONE, 9(10), e111789. (doi: 10.1371/journal.pone.0111789) (PMID:25356547) (PMCID:PMC4214795)

Kioumourtzoglou, D., Gould, G. W. and Bryant, N. J. (2014) Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism. Molecular and Cellular Biology, 34(7), pp. 1271-1279. (doi: 10.1128/MCB.01203-13)

Bhutta, M. S., McInerny, C. J. and Gould, G. W. (2014) ESCRT function in cytokinesis: location, dynamics and regulation by mitotic kinases. International Journal of Molecular Sciences, 15(12), pp. 21723-21739. (doi: 10.3390/ijms151221723) (PMID:25429432) (PMCID:PMC4284674)

Kioumourtzoglou, D., Sadler, J. B.A., Black, H. L., Berends, R., Wellburn, C., Bryant, N. J. and Gould, G. W. (2014) Studies of the regulated assembly of SNARE complexes in adipocytes. Biochemical Society Transactions, 42(5), pp. 1396-1400. (doi: 10.1042/BST20140114)

Neto, H., Kaupisch, A., Collins, L.L. and Gould, G.W. (2013) Syntaxin 16 is a master recruitment factor for cytokinesis. Molecular Biology of the Cell, 24(23), pp. 3663-3674. (doi: 10.1091/mbc.E13-06-0302) (PMID:24109596) (PMCID:PMC3842993)

Roccisana, J., Sadler, J.B.A., Bryant, N.J. and Gould, G.W. (2013) Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45. Molecular Biology of the Cell, 24(15), pp. 2389-97. (doi: 10.1091/mbc.E13-01-0011)

Sadler, J.B., Bryant, N.J., Gould, G.W. and Welburn, C.R. (2013) Posttranslational modifications of GLUT4 affect its subcellular localization and translocation. International Journal of Molecular Sciences, 14(5), pp. 9963-9978. (doi: 10.3390/ijms14059963)

Cunningham, M. R., McIntosh, K. A., Pediani, J. D. , Robben, J., Cooke, A. E., Nilsson, M., Gould, G. W. , Mundell, S., Milligan, G. and Plevin, R. (2012) Novel role for proteinase-activated receptor 2 (PAR2) in membrane trafficking of proteinase-activated receptor 4 (PAR4). Journal of Biological Chemistry, 287(20), pp. 16656-16669. (doi: 10.1074/jbc.M111.315911)

Collins, L.L., Simon, G., Matheson, J., Wu, C., Miller, M.C., Otani, T., Yu, X., Hayashi, S., Prekeris, R. and Gould, G.W. (2012) Rab11-FIP3 is a cell cycle-regulated phosphoprotein. BMC Cell Biology, 13(1), p. 4. (doi: 10.1186/1471-2121-13-4) (PMID:22401586) (PMCID:PMC3310825)

Aran, V., Bryant, N.J. and Gould, G.W. (2011) Tyrosine phosphorylation of Munc18c on residue 521 abrogates binding to Syntaxin 4. BMC Biochemistry, 12(19), (doi: 10.1186/1471-2091-12-19)

Bryant, N.J. and Gould, G.W. (2011) SNARE proteins underpin insulin-regulated GLUT4 traffic. Traffic, 12(6), pp. 657-664. (doi: 10.1111/j.1600-0854.2011.01163.x)

Gould, G.W. (2011) IKK : A Kinase at the Intersection of Signaling and Membrane Traffic. Science Signaling, 4(177), pe30. (doi: 10.1126/scisignal.2002186)

Neto, H., Collins, L.L. and Gould, G.W. (2011) Vesicle trafficking and membrane remodelling in cytokinesis. Biochemical Journal, 437(1), pp. 13-24. (doi: 10.1042/BJ20110153)

Neto, H. and Gould, G.W. (2011) The regulation of abscission by multi-protein complexes. Journal of Cell Science, 124(19), pp. 3199-3207. (doi: 10.1242/jcs.083949)

Gould, G.W. and Lippincott-Schwartz, J. (2009) New roles for endosomes: from vesicular carriers to multi-purpose platforms. Nature Reviews Molecular Cell Biology, 10(4), pp. 287-292. (doi: 10.1038/nrm2652)

Aran-Ponte, V., Brandie, F.M., Boyd, A.R., Kantidakis, T., Rideout, E.J., Kelly, S.M., Gould, G.W. and Bryant, N.J. (2009) Characterization of two distinct binding modes between syntaxin 4 and Munc18c. Biochemical Journal, 419(Pt3), pp. 655-660. (doi: 10.1042/BJ20082293) (PMID:19193195)

Simon, G.C., Schonteich, E., Wu, C.C., Piekny, A., Ekiert, D., Yu, X., Gould, G.W. , Glotzer, M. and Prekeris, R. (2008) Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis. EMBO Journal, 27(13), pp. 1791-1803. (doi: 10.1038/emboj.2008.112) (PMID:18511905) (PMCID:PMC2486418)

Brandie, F.M., Aran-Ponte, V., Verma, A., McNew, J.A., Bryant, N.J. and Gould, G.W. (2008) Negative regulation of syntaxin4/SNAP-23/VAMP2-mediated membrane fusion by Munc18c In Vitro. PLoS ONE, 3(12), e4074. (doi: 10.1371/journal.pone.0004074) (PMID:19116655) (PMCID:PMC2605266)

Prekeris, R. and Gould, G.W. (2008) Breaking up is hard to do - membrane traffic in cytokinesis. Journal of Cell Science, 121(10), pp. 1569-1576. (doi: 10.1242/jcs.018770)

Yu, X., Prekeris, R. and Gould, G. (2007) Role of endosomal Rab GTPases in cytokinesis. European Journal of Cell Biology, 86, pp. 25-35. (doi: 10.1016/j.ejcb.2006.10.002)

Choudhury, A., Marks, D., Proctor, K., Gould, G. and Pagano, R. (2006) Regulation of caveolar endocytosis by syntaxin 6-dependent delivery of membrane components to the cell surface. Nature Cell Biology, 8, 317-U6. (doi: 10.1038/ncb1380)

Clarke, M., Ewart, M.A., Santy, L.C., Prekeris, R. and Gould, G.W. (2006) ACRP30 is secreted from 3T3-L1 adipocytes via a Rab11-dependent pathway. Biochemical and Biophysical Research Communications, 342, pp. 1361-1367. (doi: 10.1016/j.bbrc.2006.02.102) (PMID:16516854)

Proctor, K., Miller, S., Bryant, N. and Gould, G. (2006) Syntaxin 16 controls the intracellular sequestration of GLUT4 in 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications, 347, pp. 433-438. (doi: 10.1016/j.bbrc.2006.06.135)

Collison, M., James, D., Graham, D., Holman, G., Connell, J., Dominiczak, A.F., Gould, G. and Salt, I. (2005) Reduced insulin-stimulated GLUT4 bioavailability in stroke-prone spontaneously hypertensive rats. Diabetologia, 48(3), pp. 539-546. (doi: 10.1007/s00125-005-1674-x)

Ewart, M.-A., Clarke, M., Kane, S., Chamberlain, L.H. and Gould, G.W. (2005) Evidence for a role of the exocyst in insulin-stimulated Glut4 trafficking in 3T3-L1 adipocytes. Journal of Biological Chemistry, 280, pp. 3812-3816. (doi: 10.1074/jbc.M409928200) (PMID:15550383)

Fielding, A.B., Schonteich, E., Matheson, J., Wilson, G., Yu, X.Z., Hickson, G.R.X., Srivastava, S., Baldwin, S.A., Prekeris, R. and Gould, G.W. (2005) Rab11-FIP3 and FIP4 interact with Arf6 and the Exocyst to control membrane traffic in cytokinesis. EMBO Journal, 24, pp. 3389-3399. (doi: 10.1038/sj.emboj.7600803) (PMID:16148947) (PMCID:PMC1276165)

Matheson, J., Yu, X., Fielding, A. and Gould, G. (2005) Membrane traffic in cytokinesis. Biochemical Society Transactions, 33, pp. 1290-1294.

McGettigan, J. et al. (2005) Insect renal tubules constitute a cell-autonomous immune system that protects the organism against bacterial infection. Insect Biochemistry and Molecular Biology, 35, pp. 741-754. (doi: 10.1016/j.ibmb.2005.02.017) (PMID:15894191)

Salaun, C., Gould, G. and Chamberlain, L. (2005) Lipid raft association of SNARE proteins regulates exocytosis in PC12 cells. Journal of Biological Chemistry, 280, pp. 19449-19453. (doi: 10.1074/jbc.M501923200)

Salaun, C., Gould, G. and Chamberlain, L. (2005) The SNARE proteins SNAP-25 and SNAP-23 display different affinities for lipid rafts in PC12 cells - Regulation by distinct cysteine-rich domains. Journal of Biological Chemistry, 280, pp. 1236-1240. (doi: 10.1074/jbc.M410674200)

Tsuboi, T., Ravier, M.A., Xie, H., Ewart, M.-A., Gould, G.W. , Baldwin, S.A. and Rutter, G.A. (2005) Mammalian exocyst complex is required for the docking step of insulin vesicle exocytosis. Journal of Biological Chemistry, 280(27), pp. 25565-25570. (doi: 10.1074/jbc.M501674200)

Wilson, G., Fielding, A., Simon, G., Yu, X., Andrews, P., Hames, R., Frey, A., Peden, A., Gould, G. and Prekeris, R. (2005) The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis. Molecular Biology of the Cell, 16, pp. 849-860.

Litherland, G., Hajduch, E., Gould, G. and Hundal, H. (2004) Fructose transport and metabolism in adipose tissue of Zucker rats: Diminished GLUT5 activity during obesity and insulin resistance. Molecular and Cellular Biochemistry, 261, pp. 23-33.

Hickson, G., Matheson, J., Riggs, B., Maier, V., Fielding, A., Prekeris, R., Sullivan, W., Barr, F. and Gould, G. (2003) Arfophilins are dual Arf/Rab 11 binding proteins that regulate recycling endosome distribution and are related to Drosophila nuclear fallout. Molecular Biology of the Cell, 14, pp. 2908-2920. (doi: 10.1091/mbc.E03-03-0160)

Morrow, V., Foufelle, F., Connell, J., Petrie, J. , Gould, G. and Salt, I. (2003) Direct activation of AMP-activated protein kinase stimulates nitric-oxide synthesis in human aortic endothelial cells. Journal of Biological Chemistry, 278(34), pp. 31629-31639. (doi: 10.1074/jbc.M212831200)

Perera, H., Clarke, M., Morris, N., Hong, W., Chamberlain, L. and Gould, G. (2003) Syntaxin 6 regulates Glut4 trafficking in 3T3-L1 adipocytes. Molecular Biology of the Cell, 14, pp. 2946-2958. (doi: 10.1091/mbc.E02-11-0722)

Perry, C., Palmer, T., Cleland, S., Morton, I., Salt, I. , Petrie, J. , Gould, G. and Connell, J. (2003) Decreased insulin sensitivity during dietary sodium restriction is not mediated by effects of angiotensin II on insulin action. Clinical Science, 105(2), pp. 187-194. (doi: 10.1042/CS20020320)

Riggs, B., Rothwell, W., Mische, S., Hickson, G., Matheson, J., Hays, T., Gould, G. and Sullivan, W. (2003) Actin cytoskeleton remodeling during early Drosophila furrow formation requires recycling endosomal components Nuclear-fallout and RAb11. Journal of Cell Biology, 163, pp. 143-154. (doi: 10.1083/jcb.200305115)

Chamberlain, L. and Gould, G. (2002) The vesicle- and target-SNARE proteins that mediate Glut4 vesicle fusion are localized in detergent-insoluble lipid rafts present on distinct intracellular membranes. Journal of Biological Chemistry, 277, pp. 49750-49754. (doi: 10.1074/jbc.M206936200)

Kelly, C.J.G., Lyall, H., Petrie, J.R. , Gould, G.W. , Connell, J.M.C., Rumley, A., Lowe, G.D.O. and Sattar, N. (2002) A specific elevation in tissue plasminogen activator antigen in women with polycystic ovarian syndrome. Journal of Clinical Endocrinology and Metabolism, 87(7), pp. 3287-3290. (doi: 10.1210/jc.87.7.3287)

Kelly, C., Speirs, A., Gould, G., Petrie, J. , Lyall, H. and Connell, J. (2002) Altered vascular function in young women with polycystic ovary syndrome. Journal of Clinical Endocrinology and Metabolism, 87(2), pp. 742-746. (doi: 10.1210/jc.87.2.742)

Chamberlain, L., Burgoyne, R. and Gould, G. (2001) SNARE proteins are highly enriched in lipid rafts in PC12 cells: Implications for the spatial control of exocytosis. Proceedings of the National Academy of Sciences of the United States of America, 98(10), pp. 5619-5624. (doi: 10.1073/pnas.091502398)

Chamberlain, L., Graham, M., Kane, S., Jackson, J., Maier, V., Burgoyne, R. and Gould, G. (2001) The synaptic vesicle protein, cysteine-string protein, is associated with the plasma membrane in 3T3-L1 adipocytes and interacts with syntaxin 4. Journal of Cell Science, 114, pp. 445-455.

James, D.J., Cairns, F., Salt, I.P. , Murphy, G.J., Dominiczak, A.F., Connell, J.M.C. and Gould, G.W. (2001) Skeletal muscle of stroke-prone spontaneously hypertensive rats exhibits reduced insulin-stimulated glucose transport and elevated levels of caveolin and flotillin. Diabetes, 50(9), pp. 2148-2156. (doi: 10.2337/diabetes.50.9.2148)

Kelly, C., Lyall, H., Petrie, J. , Gould, G., Connell, J. and Sattar, N. (2001) Low grade chronic inflammation in women with polycystic ovarian syndrome. Journal of Clinical Endocrinology and Metabolism, 86(6), pp. 2453-2455. (doi: 10.1210/jc.86.6.2453)

MacKenzie, C.J., Wakefield, J.M., Cairns, F., Dominiczak, A.F. and Gould, G.W. (2001) Regulation of glucose transport in aortic smooth muscle cells by cAMP and cGMP. Biochemical Journal, 353, pp. 513-519.

Petrie, J. , Collison, M., Connell, J., Gould, G. and Dominiczak, A. (2001) CD36 deficiency and insulin resistance. Lancet, 358(9277), pp. 242-243. (doi: 10.1016/S0140-6736(01)05428-9)

Collison, M., Campbell, I., Salt, I. , Dominiczak, A. , Connell, J., Lyall, H. and Gould, G. (2000) Sex hormones induce insulin resistance in 3T3-L1 adipocytes by reducing cellular content of IRS proteins. Diabetologia, 43(11), pp. 1374-1380. (doi: 10.1007/s001250051541)

Salt, I. , Connell, J. and Gould, G. (2000) 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes. Diabetes, 49(10), pp. 1649-1656. (doi: 10.2337/diabetes.49.10.1649)

Thomson, F. J. , Jess, T. J., Moyes, C., Plevin, R. and Gould, G. (1997) Characterization of the intracellular signalling pathways that underlie growth-factor-stimulated glucose transport in Xenopus oocytes: evidence for ras- and rho-dependent pathways of phosphatidylinositol 3-kinase activation. Biochemical Journal, 325(3), pp. 637-643. (PMID:9271083)

Jess, T. J., Belham, C. M., Thomson, F. J. , Scott, P. H., Plevin, R. J. and Gould, G. (1996) Phosphatidylinositol 3′-kinase, but not p70 ribosomal S6 kinase, is involved in membrane protein recycling: Wortmannin inhibits glucose transport and downregulates cell-surface transferrin receptor numbers independently of any effect on fluid-phase endocytosis in fibroblasts. Cellular Signalling, 8(4), pp. 297-304. (doi: 10.1016/0898-6568(96)00054-X) (PMID:8842531)

Livingstone, C. et al. (1996) Hormonal regulation of the insulin-responsive glucose transporter, GLUT4: some recent advances. Proceedings of the Nutrition Society, 55(1B), pp. 179-190. (doi: 10.1079/PNS19960019) (PMID:8832790)

Thomson, F. j. , Moyes, C., Scott, P. H., Plevin, R. and Gould, G. W. (1996) Lysophosphatidic acid stimulates glucose transport in Xenopus oocytes via a phosphatidylinositol 3'-kinase with distinct properties. Biochemical Journal, 316(1), pp. 161-166. (PMID:8645200) (PMCID:PMC1217317)

Gould, G. W. , Cuenda, A., Thomson, F. J. and Cohen, P. (1995) The activation of distinct mitogen-activated protein kinase cascades is required for the stimulation of 2-deoxyglucose uptake by interleukin-1 and insulin-like growth factor-1 in KB cells. Biochemical Journal, 311(3), pp. 735-738. (PMID:7487926)

Book Sections

Rezig, I. M., Bremner, S. K., Bhutta, M. S., Salt, I. P. , Gould, G. W. and McInerny, C. J. (2019) Genetic and cytological methods to study ESCRT cell cycle function in fission yeast. In: Culetto, E. and Legouis, R. (eds.) The ESCRT Complexes: Methods and Protocols. Series: Methods in molecular biology (1998). Humana Press: New York, pp. 239-250. ISBN 9781493994915 (doi:10.1007/978-1-4939-9492-2_18)

Kioumourtzoglou, D., Gould, G. W. and Bryant, N. J. (2018) Proximity ligation assay to study the GLUT4 membrane trafficking machinery. In: Lindkvist-Petersson, K. and Hansen, J. S. (eds.) Glucose Transport: Methods and Protocols. Series: Methods in molecular biology (1713). Humana Press: New York, pp. 217-227. ISBN 9781493975068 (doi:10.1007/978-1-4939-7507-5_16)

This list was generated on Thu Nov 26 08:55:46 2020 GMT.

Grants

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Development of a model of insulin-stimulated glucose transport in human cardiomyocytes
    British Heart Foundation
    2018 - 2020
     
  • Sx16 phosphorylation as a regulatory mechanisms controlling insulin-stimulated gluocse transport
    Diabetes UK
    2018 - 2021
     
  • BBSRC iGEM sponsorship 18/19-20/21
    Biotechnology and Biological Sciences Research Council
    2018 - 2021
     
  • Wellcome 2018 iGEM
    Wellcome Trust
    2018 - 2019
     
  • Syntaxin Phosphorylation as an insulin-regulatory node of Glut4 sorting in human muscle of individuals with type 2 diabetes
    The Novo Nordisk UK Research Foundation
    2017 - 2020
     
  • How does Cholesterol regulate insulin action in adipocytes?
    Diabetes UK
    2016 - 2019
     
  • Control of GLUT4 sorting by Syntaxin 16 and mVps45 in adipocytes
    Diabetes UK
    2014 - 2016
     
  • Commonwealth Fellowship
    Commonwealth Scholarship Commission in the UK
    2012 - 2012
     
  • How does tyrosine phosphorylation of SNARE proteins control GLUT4 vesicle fusion with the plasma membrane?
    Diabetes UK
    2012 - 2015
     
  • Role of ubiquitination as a transient modification in regulated protein trafficking
    Medical Research Council
    2012 - 2015
     
  • The sensitive assessment of the activity of cellular signalling pathways regulating insulin action and the cardiovascular complications of diabetes
    Diabetes UK
    2011 - 2014
     
  • Investigation of the role of Syntaxin 16 and mVps45 in cytokinesis
    Cancer Research UK
    2011 - 2015
     
  • How does Syntaxin 16 control Glut4 sorting?
    Diabetes Research & Wellness Foundation
    2011 - 2012
     
  • How does mVps45 regulate Glut4 sorting in adipocytes
    Diabetes UK
    2010 - 2012
     
  • Regulation and role of the Exocyst complex in cytokinesis.
    Cancer Research UK
    2010 - 2011
     
  • Phosphoryaltion of FIP3 and its consequence for cytokinesis
    Cancer Research UK
    2009 - 2012
     
  • Tyrosine phosphorylation of Munc18c in insulin action
    Diabetes Research & Wellness Foundation
    2009 - 2009
     
  • Translational analysis of the role of synaptotagmins in the adipocyte, and their relationship to insulin action
    Scottish Executive Health Department
    2008 - 2010
     
  • The role of mvps45 in Glut4 trafficking in adipocytes
    Diabetes UK
    2007 - 2007
     
  • Functional analysis of Munc18c/Syntaxin4 interactions
    Biotechnology and Biological Sciences Research Council
    2005 - 2008
     
  • The role of Rab 11-FIP3 and Rab 11-FIP4 in cytokinesis
    Biotechnology and Biological Sciences Research Council
    2005 - 2009
     
  • The role of arfophilins in cytokinesis
    Wellcome Trust
    2004 - 2007
     
  • Functional analysis of the adipocyte secreted proteome
    Biotechnology and Biological Sciences Research Council
    2004 - 2007
     
  • Compartmentalisation of insulin signalling in human adipocytes - role of raft associated structures
    Scottish Executive Health Department
    2003 - 2007
     
  • The role of synaptotagmins in GLUT4 translocation in the adipocyte
    Diabetes Research & Wellness Foundation
    2003 - 2006
     
  • The role of synaptotoagmins in GLUT4 translocation in the adipocyte Dr S Miller
    Medical Research Council
    2003 - 2006
     
  • Subcellular compartmentalisation analysis by confocal microscopy
    Biotechnology and Biological Sciences Research Council
    2003 - 2007
     
  • Lipid rafts and the spatial control of exocytosis
    Biotechnology and Biological Sciences Research Council
    2002 - 2005
     
  • Role of Arf5 and Arf6 in regulated membrane trafficking in adipose cells
    Biotechnology and Biological Sciences Research Council
    2000 - 2005
     

Supervision

    The interface of insulin signalling and membrane trafficking.
    Insulin binding activates the intrinsic tyrosine kinase activity of the receptor beta-subunit. Although the essential role of two major targets for this kinase, IRS1 and IRS2, in metabolic regulation is clear, little is known about the role of insulin-stimulated tyrosine phosphorylation of other proteins. Syntaxin 4 and Munc18c, proteins intimately involved in the insulin-stimulated translocation of the facilitative glucose transporter, GLUT4, to the plasma membrane are both tyrosine-phosphorylated within minutes of insulin stimulation. Our recent work has established that these phosphorylation events have key functional consequences. We now wish to determine how the signalling and trafficking machinery is co-located within cells, and to determine whether alterations in this underly insulin resistance.

    How does phosphorylation of Sx16 regulate GLUT4 sorting?
    A key action of insulin on adipose and muscle cells is to initiate a signalling cascade that changes the trafficking facilitative glucose transporter GLUT4, releasing it from a specialised intracellular store and delivering it to the cell surface. Individuals with insulin-resistance and Type 2 Diabetes exhibit significantly impaired insulin-stimulated glucose transport, and this appears to be largely due to a failure of GLUT4 translocation. Understanding how GLUT4 trafficking is controlled at the molecular level is therefore an essential step in the development of effective therapies against the current global epidemic of insulin-resistance and diabetes.
    This project seeks to understand the sorting of GLUT4 into its specialised storage compartment. We will build our published and unpublished data to further our understanding of the role that the SNARE protein Syntaxin-16 plays in this process, and in particular how regulated phosphorylation of Syntaxin-16 controls GLUT4 sorting.

    Control of GLUT4 dispersal by regulation of the plasma membrane lipidome.
    It has recently been discovered that in addition to regulating delivery of GLUT4 from intracellular stores to the plasma membrane, insulin also regulates the spatial organisation of GLUT4 at the cell surface.  This project builds upon findings from our lab to understand the molecular mechanisms that control this recently described effect of insulin.  The project will investigate how patches at the cell surface of adipocytes that are enriched in phosphatidylinositol-4-phosphate contribute to GLUT4’s localisation and functionality and also how these patches are regulated by insulin. The potential that this aspect of insulin action in impaired in Type-2 diabetes will also be examined.

    A Model for Diabetic Cardiomyopathy.
    Metabolic disorders such as obesity, insulin resistance and type 2 diabetes (T2DM) mellitus are all linked to the development of cardiovascular disease. The World Health Organisation has projected that T2DM will be the seventh leading cause of death by 2030. Cardiovascular disease represents the single biggest cause of death in these patients, and all present studies suggest that diabetes has a negative effect on cardiac muscle independently of concurrent vascular disease. This project will use cardiomyocytes derived from induced pluripotent stem cells and model the effects of a prediabetic milieu on differentiation, gene expression and insulin sensitivity in this system in order to better understand changes associated with diabetic cardiomyopathy.

    Excitation contraction coupling and glucose transport.
    A key action of insulin is to increase glucose uptake into cardiomyocytes via GLUT4 translocation to the cell surface. In cardiac tissue, exercise and electrophysiological activity can also promote GLUT4 translocation. However the mechanism(s) that couple electrical activity to the control of GLUT4 availability at the cell surface remain unidentified. This project will test the hypothesis that a direct link between channels on the cell surface and the GLUT4 vesicle fusion machinery controls GLUT4 vesicle exocytosis, and that this is impaired in diabetic cardiomyopathy. We will also develop ontogenetic approaches to regulate GLUT4 activity in muscle and cardiomyocytes.

Teaching

  • Masters: Lectures on 'Endocrinology' and 'Diabetes' M.Sc. courses.
  • Masters: Discussion facilitator on 'Endocrinology' and 'Diabetes' M.Sc. courses.
  • Level 4: Option Central Approaches in Biochemistry lectures on Membrane biogenesis, function and transport
  • Level 4: Option Central Approaches in Biochemistry - tutorial leader
  • Level 4: Option Cell Compartmentalisation and Function Coordinator
  • Level 4: Option Cell Compartmentalisation and Function lectures on membrane trafficking, SNARE protein biology and membrane scission.
  • Level 3: Biochemistry Overall Course Coordinator.
  • Level 3: Block Leader; Membranes and Filaments.
  • Level 3: Lab classes for Biochemistry and Molecular Cell Biology students.
  • Level 3: Lectures on Bioenergetics (Biochemistry students) and Membrane Biology (Biochemistry, Genetics and Molecular Cell biology students)
  • Level 3: Data handling sessions; Careers Sessions; Employability workshops.
  • Level 3: "How to read a paper"
  • Level 3: Tutorial leader.
  • Level 2: Overall Coordinator 'Genes. Molecules and Cells-2', compulsory second year course for all bimolecular science students.
  • Level 2: Lectures on 'Genes, Molecules and Cells-2' (Protein Structure and Function; Signalling and Cell Compartmentalisation).
  • Level 2: Laboratory classes in 'Genes. Molecules and Cells-2'
  • Level 2: Lectures on 'Fundamental Topics in Biology-2' compulsory course.
  • Level 2: Block leader on 'Fundamental Topics in Biology-2' course.
  • Level 2: Laboratory classes on 'Fundamental Topics in Biology-2' course.
  • Level 2: Fundamental Topics in Biology 2; Block Leader "Systems to Cells"
  • Academic Advisor of Studies.
  • Member College Teaching and Learning Committee.
  • Member and Chair, Institute Teaching and Learning Committee.

Additional information

Editorial Board

  • 2017 - present: Scientific Reports - Editorial Board Member
  • 2017 - present: PeerJ - Editorial Board Member
  • 2005 - 2010: Biochemical Journal
  • 2004 - 2010: Endocrinology
  • 1995 - 1998: Biochemica et Biophysica Acta
  • 1992 - 1997: Biochemical Journal

Grant Advisory Board

  • 2009 - present: Qatar Medical Research Foundation
  • 2004: Scottish Executive - Director of Chief Scientists Office Sub-Committee to Review Translational Diabetes Research in Scotland
  • 2002 - 2003: Scottish Executive - Chief Scientists Office Committee to Review Needs of Diabetes Research in Scotland
  • 2002: BBSRC - Chair, Sequencing Needs Review Panel
  • 2001 - 2004: BBSRC - Strategy Board
  • 1999: Diabetes UK - Studentship & Fellowship Committee
  • 1999 - 2004: BBSRC - Biochemistry & Cell Biology Committee Member, Chairman 2001-04

Invited International Presentations

  • 2015: Glasgow, UK - Glasgow Diabetes UK Professional Conference: 'SNARE proteins in the control of glucose transport'.
  • 2014: London, UK - London Diabetes UK Professional Conference - basic science presentation judge.
  • 2010: Oxford, UK - Co-organiser, "Cell Organization through the Cell Cycle." British Society of Cell Biology satellite meeting.
  • 2009: Glasgow, UK - Invited Speaker, CR-UK Beaton Meetin "Vesicle Trafficking in Cancer"
  • 2009: Colorado, USA - Invited speaker, FASEB 8th Glucose Transporter Biology.
  • 2009: Vienna, Austria - Invited Speaker, European Association for the Study of Diabetes Conference
  • 2009: Oxford, UK - Invited speaker "Cell Organization through the Cell Cycle." British Society of Cell Biology satellite meeting.
  • 2008: Edinburgh, UK - Co-organiser, "Mechanics and Control of Cytokinesis meeting"
  • 2007: Il Ciocco, Italy - FASEB Summer Research Conference on Arf GTPases
  • 2007: Colorado, USA - FASEB Summer Research Conference on Glucose Transporter Biology
  • 2005: Biosciences 2005 - Keynote Speaker
  • 2004: Vermont, USA. - American Society for Cell Biology Satellite Symposium, Cytokinesis
  • 2003: Glasgow Cell Biology Inaugural Meeting
  • 2003: Glasgow, UK. - Society for Experimental Biology
  • 2002: San Francisco, USA. - American Society for Cell Biology
  • 2002: Heidelberg, Germany. - EMBL Predoctoral Student Symposium
  • 2001: Colorado, USA. - FASEB Meeting, Glucose transporter biology.
  • 2001: American Diabetes Association
  • 2001: Diabetes UK

Professional Learned Society

  • 2005 - present: Biochemical Society - Member
  • 2005 - present: American Endocrine Society - Member
  • 2004 - present: American Society of Biochemistry and Molecular Biology - Member
  • 2002 - present: British Cell Biology Society - Regular Member
  • 1999 - present: American Society of Cell Biology - Regular Member
  • 1998 - present: Lister Institute of Preventive Medicine
  • 1992 - present: American Diabetes Association

Research Fellowship

  • 2004 - 2007: Wellcome Trust Research Leave Fellowship
  • 1992 - 1998: Lister Institute Fellowship

R244 Level 2, Henry Wellcome Lab of Cell Biol., Davidson Building, Glasgow G12 8QQ,