Dr Anthony Dornan

  • Research Technician (Institute of Molecular Cell & Systems Biology Administration)

Biography

Key Skills 

Core Funded

  • Expert in immunofluorescent and standard histochemical techniques; light, phase-contrast, differential interference contrast (Nomarski) and confocal microscopy; and with the processing of images and data generated. Expert in the generation of 3D (x, y, z) or 4D advanced confocal applications such as Fluorescence Energy Transfer (FRET), photoactivation, colocalization analysis, and Recovery After Photobleaching (FRAP). 
  • Expert in micro-dissection and preparation of tissue samples for disparate molecular genetic processes such as isolation of RNA/DNA or for use in image acquisition etc. 
  • Expert in the culture and maintenance of various invertebrate model organisms and the application of novel assay techniques to disparate invertebrate systems. Specialism in Drosophila genetics and various Drosophila experimental/behavioural assays and statistical analyses of subsequent data generated. 
  • Lab manager & administrative experience including budgetary oversight/purchasing, implementation of health & safety guidelines, training and mentoring of staff and students etc. 

 


Research interests

My research focuses on applying molecular genetic techniques, predominantly using the model organism Drosophila and in particular their Malpighian (renal) tubules, to address fundamental questions in the physiology of cell signalling, transport and stress responses (from environmental, pathogenic, and xenobiotic challenges). I also employ other invertebrate systems, both beneficial and pest species, to examine systemic effects of various challenges in the development of biocontrol systems for food security. 


Publications

List by: Type | Date

Jump to: 2020 | 2019 | 2018 | 2016 | 2012
Number of items: 8.

2020

Cabrero, P. , Terhzaz, S. , Dornan, A. J. , Ghimire, S., Holmes, H. L., Turin, D. R., Romero, M. F., Davies, S. and Dow, J. A.T. (2020) Specialized stellate cells offer a privileged route for rapid water flux in Drosophila renal tubule. Proceedings of the National Academy of Sciences of the United States of America, 117(3), pp. 1779-1787. (doi: 10.1073/pnas.1915943117) (PMID:31907321)

2019

Alford, L., Marley, R., Dornan, A. , Dow, J. A.T. , Nachman, R. J. and Davies, S. A. (2019) Desiccation, thermal stress and associated mortality in Drosophila fruit flies induced by neuropeptide analogue treatment. Journal of Pest Science, 92, pp. 1123-1137. (doi: 10.1007/s10340-019-01100-0)

Alford, L., Marley, R., Dornan, A. , Pierre, J.-S., Dow, J. A.T. , Nachman, R. J. and Davies, S. A. (2019) Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance. Pest Management Science, 75(6), pp. 1750-1759. (doi: 10.1002/ps.5372) (PMID:30734498) (PMCID:PMC6593983)

Davies, S.-A. , Cabrero, P. , Marley, R., Martinez-Corrales, G., Ghimire, S., Dornan, A. J. and Dow, J. A.T. (2019) Epithelial function in the Drosophila Malpighian tubule: an in vivo renal model. In: Vainio, S. (ed.) Kidney Organogenesis: Methods and Protocols. Series: Methods in molecular biology (1926). Humana Press: New York, NY, pp. 203-221. ISBN 9781493990207 (doi:10.1007/978-1-4939-9021-4_17)

2018

Terhzaz, S. , Alford, L., Yeoh, J. G.C. , Marley, R., Dornan, A. J. , Dow, J. A.T. and Davies, S. A. (2018) Renal neuroendocrine control of desiccation and cold tolerance by Drosophila suzukii. Pest Management Science, 74(4), pp. 800-810. (doi: 10.1002/ps.4663) (PMID:28714258) (PMCID:PMC5888198)

2016

Halberg, K. A., Rainey, S. M., Veland, I. R., Neuert, H., Dornan, A. J. , Klämbt, C., Davies, S.-A. and Dow, J. A.T. (2016) The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules. Nature Communications, 7, 11266. (doi: 10.1038/ncomms11266) (PMID:27072072) (PMCID:PMC4833865)

Cannell, E., Dornan, A. J. , Halberg, K. A., Terhzaza, S. , Dow, J. A.T. and Davies, S. A. (2016) The corticotropin-releasing factor-like diuretic hormone 44 (DH44) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster. Peptides, 80, (doi: 10.1016/j.peptides.2016.02.004) (PMID:26896569) (PMCID:PMC4889782)

2012

Rezaval, C., Pavlou, H.J., Dornan, A.J. , Chan, Y., Kravitz, E.A. and Goodwin, S.F. (2012) Neural circuitry underlying Drosophila female postmating behavioral responses. Current Biology, 22(13), pp. 1155-1165. (doi: 10.1016/j.cub.2012.04.062)

This list was generated on Thu Jul 9 01:38:40 2020 BST.
Number of items: 8.

Articles

Cabrero, P. , Terhzaz, S. , Dornan, A. J. , Ghimire, S., Holmes, H. L., Turin, D. R., Romero, M. F., Davies, S. and Dow, J. A.T. (2020) Specialized stellate cells offer a privileged route for rapid water flux in Drosophila renal tubule. Proceedings of the National Academy of Sciences of the United States of America, 117(3), pp. 1779-1787. (doi: 10.1073/pnas.1915943117) (PMID:31907321)

Alford, L., Marley, R., Dornan, A. , Dow, J. A.T. , Nachman, R. J. and Davies, S. A. (2019) Desiccation, thermal stress and associated mortality in Drosophila fruit flies induced by neuropeptide analogue treatment. Journal of Pest Science, 92, pp. 1123-1137. (doi: 10.1007/s10340-019-01100-0)

Alford, L., Marley, R., Dornan, A. , Pierre, J.-S., Dow, J. A.T. , Nachman, R. J. and Davies, S. A. (2019) Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance. Pest Management Science, 75(6), pp. 1750-1759. (doi: 10.1002/ps.5372) (PMID:30734498) (PMCID:PMC6593983)

Terhzaz, S. , Alford, L., Yeoh, J. G.C. , Marley, R., Dornan, A. J. , Dow, J. A.T. and Davies, S. A. (2018) Renal neuroendocrine control of desiccation and cold tolerance by Drosophila suzukii. Pest Management Science, 74(4), pp. 800-810. (doi: 10.1002/ps.4663) (PMID:28714258) (PMCID:PMC5888198)

Halberg, K. A., Rainey, S. M., Veland, I. R., Neuert, H., Dornan, A. J. , Klämbt, C., Davies, S.-A. and Dow, J. A.T. (2016) The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules. Nature Communications, 7, 11266. (doi: 10.1038/ncomms11266) (PMID:27072072) (PMCID:PMC4833865)

Cannell, E., Dornan, A. J. , Halberg, K. A., Terhzaza, S. , Dow, J. A.T. and Davies, S. A. (2016) The corticotropin-releasing factor-like diuretic hormone 44 (DH44) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster. Peptides, 80, (doi: 10.1016/j.peptides.2016.02.004) (PMID:26896569) (PMCID:PMC4889782)

Rezaval, C., Pavlou, H.J., Dornan, A.J. , Chan, Y., Kravitz, E.A. and Goodwin, S.F. (2012) Neural circuitry underlying Drosophila female postmating behavioral responses. Current Biology, 22(13), pp. 1155-1165. (doi: 10.1016/j.cub.2012.04.062)

Book Sections

Davies, S.-A. , Cabrero, P. , Marley, R., Martinez-Corrales, G., Ghimire, S., Dornan, A. J. and Dow, J. A.T. (2019) Epithelial function in the Drosophila Malpighian tubule: an in vivo renal model. In: Vainio, S. (ed.) Kidney Organogenesis: Methods and Protocols. Series: Methods in molecular biology (1926). Humana Press: New York, NY, pp. 203-221. ISBN 9781493990207 (doi:10.1007/978-1-4939-9021-4_17)

This list was generated on Thu Jul 9 01:38:40 2020 BST.