MicroScale Thermophoresis (MST) is a new powerful technology that measures the strength of interaction by detecting variations in fluorescence signal as a result of an Infra red-laser induced temperature change within glass capillaries filled with biomolecules. The range of the variation in the fluorescence signal correlates with the binding of a ligand to the fluorescent target.
We have two labelling kits each kit takes approx 30mins to label a protein, which can then be stored -20 degrees in small aliquots for up to 25 experiments.
The Monolith NT Protein Labelling Kit RED-label can label small amounts (2-20uM) of purified protein. Contains NHS- ester chemistry reacts with primary amines of proteins to form highly stable dye-protein conjugates. Primary amine groups found on basic amino acid residues. The dye shows fluorescence and emission at approx. 650 and 670nm.
The Monolith His-Tag Labelling Kit RED- is site specific, purification free and can label small amounts of His tagged proteins . The dye binds to His tags with six histidines or more with a Kdin the single digit nM range. The dye shows fluorescence and emission at approx. 650 and 670nm.
Contact June Southall