How stable is my protein?

Circular Dichroism (CD) can be used to determine the thermal denaturation profile of a protein. A sample is heated from 10-80 degrees and scanned at increments of 10oC to study the unfolding and refolding as the protein is heated and cooled down again.

Differential Scanning Calorimetry (DSC)  can be used to heat a sample in the range 10-90oC, it is a direct measurement of the heat absorbed during denaturation, it is label free. Tm –is the melting transition temperature of a protein, it is an indicator of stability as this is the point where a protein is 50% native and 50% unfolded.